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1.
为探讨内皮素(ET)、一氧化氮(NO)在高血压发病中的作用及相互关系。选用自发性高血压大鼠(SHR)和对照WKY大鼠各10只,分别测定血浆中NO2^-/NO3^-,MDA及ET水平,分析血压与ET、NO及MDA与NO的关系。结果SHR血浆中的NO2^-/NO3^-、ET、MDA水平明显高于对照组(P〈0.01),同时SHR血压与ET水平独立相关,偏相关系数为0.8855(P〈0.01),MDA与N 相似文献
2.
目的:检测NO与O2^-共同或分别作用对兔肺动脉反应性变化的影响。方法;将制备的离体兔肺动脉循环分为NO组,NO2-组,NO+O2组和溶剂对照(NS)组。用离全血管环技术观察肺动脉对乙酰胆碱(ACh)、Ca^2+载体A23187和苯肾上腺素PE)的反应性变化。结果:肺动脉对ACh的舒张反应(gtension/mg dw)和舒张反应百分率(%)、在O2组明显降低(P〈0.01,与NS组比较);在NO 相似文献
3.
目的:了解慢性丙型肝炎患者(2',5')寡腺苷酸合成酶(2-5AS)活性在干扰素治疗过程中的动态变化及意义。方法:PEI-CELLULOSE薄板层析法。结果:(1)慢性丙型肝炎患者2-5AS基础水平比正常人显著升高(P<0.01);(2)IFNα治疗结束后HCVRNA持续阳性的病人2-5AS基础水平比HCVRNA转阴的病人要高(P<0.05);(3)IFNα治疗效果较好,HCVRNA转阴的病人2-5AS水平在用药后24h比HCVRNA未转阴的病人上升得更高(P<0.01);(4)用药后1个月2~5AS水平与IFNα治疗效果无显著相关性(P>0.05)。结论:2-5AS可以作为评价干扰素治疗效果的1个机体反应指标。 相似文献
4.
目的:检测NO与O-·2共同或分别作用对兔肺动脉反应性变化的影响。方法:将制备的离体兔肺动脉环分为NO组,O-·2组,NO+O-·2组和溶剂对照(NS)组。用离体血管环技术观察肺动脉对乙酰胆碱(ACh)、Ca2+载体A23187和苯肾上腺素(PE)的反应性变化。结果:肺动脉对ACh的舒张反应(gtension/mgdw)和舒张反应百分率(%),在O-·2组明显降低(P<0.01,与NS组比较);在NO+O-·2组显著高于O-·2组(P<0.05),但与NS组也有明显差异(P<0.05);在NO组肺动脉的舒张反应接近NS组,与O-·2组有明显差异(P<0.05)。对A23187的舒张反应(用占预收缩的百分比表示),在NS、NO+O-·2和O-·2组分别为73.67%,64.86%和59.26%。各组肺动脉对PE的收缩反应大致相同。L-精氨酸预孵育后,对PE的收缩反应在NO+O-·2组收缩降低,为预孵育前收缩的53.89%;而在O-·2组无明显变化。结论:O-·2损害了肺动脉的内皮依赖和受体依赖性舒张反应;NO同时生成时可部分逆转O-·2的损伤作用;O-·2似通过清除内源性NO或直接累及ecNOS,而发挥其损伤 相似文献
5.
糖尿病高血压者尿儿茶酚胺与动态血压的关系 总被引:7,自引:0,他引:7
目的:探讨2型糠尿病高血压者尿儿茶酚胺(UCA)与动态血压(ABP)的关系。方法:用HPLC-ECD测定30例糠尿病高血压者(HDM组)及25例糖尿病血压正常者(NDM组(UCA浓度「c(UCA)」昼夜UCA包括去甲肾上腺素(NE)、肾上腺素(E)、多巴胺(DA(,同时监测24hABP。结果:HDM与NDM2组间以及血压昼夜节律反转(n=10)与非反转者(n=43)间日、夜及24h尿的NE、E水平 相似文献
6.
培哚普利对老年高血压病患者血浆内皮素,一氧化氮的作用 总被引:2,自引:1,他引:1
为了解培哚普利(Perindopril)降压治疗对内皮素(ET)和一氧化氮(NO)的影响,测定24例老年高血压病人治疗前后血浆ET和NO稳定代谢产物NO-2/NO-3(NO-x)水平。结果显示:治疗8周后血浆ET水平从(74.05±11.09)×10-9g/L降至(62.09±12.51)×10-9g/L(P<0.05);ET/NO-x比值从(1.21±0.38)明显下降至(0.72±0.18)g/L(P<0.05);而NO-x水平明显升高,从(69.90±24.45)μmol/L升至(97.59±20.55)μmol/L(P<0.05)。提示:培垛普利的降压作用可能部分要归于ET和NO的变化 相似文献
7.
Ⅱ型糖尿病人的胰岛素抵抗与动态血压的关系 总被引:3,自引:0,他引:3
目的:分析Ⅱ型糖尿病病人的胰岛素抵抗与动态血压的关系。方法:对74例非胰岛素依赖型糖尿病(NIDDM)病人行24h动态血压监测,其中合并高血压组38人。所有病人行馒头餐试验,测定血糖、胰岛素及血脂,行相关分析。结果:高血压组较血压正常组的胰岛素敏感指数(ISI)显著降低,ISI与24h平均收缩压和舒张压呈显著负相关性,Logistic多元回归分析,ISI与高血压显著负相关(OR=0.4391P=0 相似文献
8.
目的:探讨基因重组促红细胞生成素(r-HuEPO)在治疗慢性肾衰(CRF)贫血中导致高血压的机制及防治对策。方法:对r-HuEPO治疗中出现高血压CRF血液透析贫血患者15例及对照组15例,CRF贫血动物模型,以及透析过程中出现低血压的34例患者,应用分光光度法及放免法观察用药或透析前后血浆一氧化氮(NO)代谢产物(NO2/NO3)及内皮素-1(ET-1)的变化。结果: ①CRF贫血患者及贫血鼠用r-HuEPO治疗16周后,红细胞压积(Hct)升高,血压升高,ET-1升高(P<0.01),血浆NO2/NO3下降(P<0.01),而对照组用药前后无明显变化(P>0.05)。②在用药16周后,RT- PCR测得实验组大鼠肾皮质及髓质iNOSmRNA比对照组明显减少(P<0.05)。③透析低血压患者透析后NO2/NO3含量增高,ET-1含量减少(P<0.05)。结论:研究表明r-HuEPO导致高血压与iNOSmRNA减少、合成NO减少和ET-1增加有重要的关系。 相似文献
9.
目的探讨吸烟对气道平滑肌细胞(ASMC)的影响。方法分别以0、5%、10%和30%的香烟烟雾提取物(CSE)孵育兔ASMC,测定其细胞存活率、丙二醛(MDA)含量、乳酸脱氢酶(LDH)漏出及一氧化氮(NO)稳定代谢终产物亚硝酸盐(NO-2)水平。用onewayANOVA检验及直线回归作统计学分析。结果在48hCSE显著增加了ASMC的NO-2释放(10%、30%CSE分别较对照组增加75.7%和115.3%,P<0.01);降低了细胞存活率,增加了细胞MDA含量和LDH漏出(P均<0.01),毒性损伤指标与NO-2的释放高度相关(r分别为-0.721、0.706和0.773,P均<0.01)。结论吸烟可能对ASMC具有直接损伤作用,CSE可能通过NO介导气道炎症损伤过程。 相似文献
10.
目的:阐明雌二醇对内皮细胞一氧化氮(NO)生成及一氧化氮合酶(cNOS)基因表达的影响。方法:观察雌二醇作用于原代人脐静脉内皮细胞(HUVEC)一定时间(1~24h)后的NO产量,采用Greis反应测定代谢产物NO-2的量及Northern杂交技术分析雌二醇作用于HUVEC18hcNOSmRNA的表达。结果:100、30、10及1nmol·L-1的雌二醇作用下,HUVEC分别于1、4、12及18h起,NO-2产量有明显增高。0.1nmol·L-1雌二醇作用24h内,NO-2产量与对照组比较差异无统计学意义。Northern杂交结果表明:1~100nmol·L-1雌二醇可明显促进cNOSmRNA的表达;而0.1nmol·L-1雌二醇无此效应。结论:雌二醇可促进HUVECNO的生成及cNOS基因的表达,可能为雌激素保护作用的重要机制,雌二醇作用的阈浓度可能在0.1~1nmol·L-1之间 相似文献
11.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD. 相似文献
12.
Jiageng Zhu Jun Li Ruipeng Ji Jianghao Su Mingshun Shen Zhigang Cao 《南京医科大学学报(自然科学版)》2007,21(2):125-128
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment. 相似文献
13.
Tao Ye * Zhi-quan Liu Jian-jun Mu Xi-han Fu Jun Yang Bao-lin Gao and Xiao-hong Zhang Department of Cardiology First Hospital of Xi'an Jiaotong University Xi'an Hanzhong Cardiovascular Institute Hanzhong 《中国医学科学杂志(英文版)》2004,19(4):248-251
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure. 相似文献
14.
目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险. 相似文献
15.
Jingming Zhu Ningning Hui Xiuhong Jiang Huaxing Zhang Chengzhang Fu 《南京医科大学学报(自然科学版)》2007,21(6):386-389
Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P<0.01);the MAP decreased(P<0.01)and the HR increased drastically(P<0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients. 相似文献
16.
Objective:To investigate the gene expression of osteoprotegerin(OPG) and osteoclast differentiation factor(ODF) in the bone tissue of patients with hip fracture due to osteoporosis. Methods:OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers(over 50 years old) with hip fracture(Observer Group) and 30 cases of hip facture sufferers with no osteoporosis(Control group) were analyzed with the Semi-Quantitative RT-PCR method. Results:The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion:Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption. 相似文献
17.
The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas: report of 9 cases 总被引:1,自引:0,他引:1
Shaoqin Chen Shengquan Zou Qibao Dai Peisheng Huang Weihua Cai Hong Li 《南京医科大学学报(自然科学版)》2007,21(6):398-401
Objective:To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas(SPTP). Methods:Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin(HE) staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results:Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin(9/9 cases), AAT(9/9 cases), NSE(9/9 cases), ACT(7/9 cases), CK20(2/9 cases), CgA(1/9 cases), S-100(3/gcases), PR(4/gcases), Syn(3/9 cases) and CD56(5/9cases), negative in CEA and ER. Conclusion:SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype. 相似文献
18.
Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine
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A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes. 相似文献
19.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P. 相似文献
20.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine. 相似文献