Photothermal therapy of tumors in lymph nodes using gold nanorods and near-infrared laser light

Lymph node dissection for regional nodal metastasis is a primary option, but is invasive and associated with adverse effects. The development of non-invasive therapeutic methods in preclinical experiments using mice has been restricted by the small lymph node size and the limited techniques available for non-invasive monitoring of lymph node metastasis. Here, we show that photothermal therapy (PTT) using gold nanorods (GNRs) and near-infrared (NIR) laser light shows potential as a non-invasive treatment for tumors in the proper axillary lymph nodes (proper-ALNs) of MXH10/Mo–lpr/lpr mice, which develop systemic swelling of lymph nodes (up to 13 mm in diameter, similar in size to human lymph nodes). Tumor cells were inoculated into the proper-ALNs to develop a model of metastatic lesions, and any anti-tumor effects of therapy were assessed. We found that GNRs accumulated in the tumor in the proper-ALNs 24 h after tail vein injection, and that irradiation with NIR laser light elevated tumor temperature. Furthermore, combining local or systemic delivery of GNRs with NIR irradiation suppressed tumor growth more than irradiation alone. We propose that PTT with GNRs and NIR laser light can serve as a new therapeutic method for lymph node metastasis, as an alternative to lymph node dissection.     

Circulation kinetics and biodistribution of dual-labeled polymersomes with modulated surface charge in tumor-bearing mice: Comparison with stealth liposomes

Polymersomes (Ps) based on poly(ethylene glycol)-b-poly(D,L-lactide) (PEG-PDLLA), with similar sizes (90-100 nm), but different zeta potentials (− 7.6 to − 38.7 mV) were prepared to investigate the effect of surface charge on blood circulation time and tissue distribution in tumor-bearing mice. For the in vivo studies dual labeled Ps were applied, which were obtained by encapsulating ³H-dextran 70 k in the aqueous core of Ps and by post-coupling of ¹⁴C-thioglycolic acid onto acrylated PEG chains of the Ps. Stealth liposomes (103 nm, − 6 mV) were used as a control. A substantial longer half lifetime (τ_1/2) (47.3 h) and a reduced liver uptake (27.9% of injected dose (% ID)) of Ps with a zeta potential of − 7.6 mV were observed as compared to those of stealth liposomes (10.6 h, 39.8% ID) most probably due to the presence of a relatively thicker and denser PEG brush of the Ps as compared to the liposomes. As a result of their longer circulation times a high tumor accumulation of 18.6% ID was obtained for these Ps after 3 d circulation in mice while only 11.2% ID of stealth liposomes accumulated in the tumors as a result of their relatively short τ_1/2 in blood. By increasing the zeta potential on Ps, more rapid clearance of Ps from the blood circulation was found due to an enhanced uptake by the liver. Importantly, co-localization of the two labels of Ps was observed during circulation indicating that dual labeled Ps were colloidally stable in blood without leakage of ³H-dextran. In conclusion, the results show that Ps with a slightly negative surface charge (zeta potential − 7.6 mV) are stable in the circulation and have longer circulation times and a higher tumor accumulation in mice than Ps with more negative zeta potentials or the stealth liposomes used as a control.     

单次化疗后荷瘤小鼠肿瘤细胞凋亡显像研究

目的探讨活体肿瘤细胞凋亡监测作为评价肿瘤对化疗反应的一种新方法的可行性.方法细胞凋亡分子探针99Tcm-HYNIC-Annexin Ⅴ经化学和放射化学合成获得.20～25 g昆明种小白鼠右前腋下皮下组织接种S-180肉瘤,建立荷肿瘤小鼠模型.荷瘤小鼠在环磷酰胺腹腔内给药化疗8 h、24 h、48 h、72 h后,分别尾静脉注射99Tcm-HYNIC-Annexin Ⅴ,1 h后进行单光子发射型计算机断层显像(SPECT)和体内生物分布测定,并与对照组进行比较.根据化疗后显像的最佳时间,给荷瘤小鼠尾静脉注射99Tcm-DTPA-HSA,测定体内各组织器官的血流分布情况.所有体内生物分布的实验结果应用SPSS 10.0统计学软件进行统计学分析.结果荷瘤小鼠在环磷酰胺腹腔内给药化疗后72 h进行SPECT显像时,肿瘤的显像效果最明显,且体内生物分布测定显示在化疗后72 h的肿瘤组织的放射性摄取值(1.87±0.58)最高,是对照组(1.18±0.128)的1.58倍(二者比较,P<0.05),与显像结果具有一致性.肿瘤/肌肉(T/M)放射性摄取率之比为5.83±0.799,肿瘤/血液(T/B)为1.03±0.258,与对照组比较均为P<0.05.化疗组和对照组的肿瘤组织对99Tcm-DTPA-HSA的摄取无明显差异(P>0.05).结论在进行单次环磷酰胺腹腔内给药化疗后72 h,荷瘤小鼠的肿瘤组织对 99Tcm-HYNIC-Annexin Ⅴ的摄取显著增加,细胞凋亡体内显像作为一种评价肿瘤对化疗反应的无创性的监测方法是有效、可行的.     

抗病毒药物治疗时间和剂量对慢性丙型肝炎患者治疗效果影响的研究

目的研究抗病毒药物治疗时间及剂量对慢性丙型肝炎患者治疗效果的影响。方法选择2010年6月至2014年4月接受治疗的慢性丙型肝炎患者90例。所有患者均采用聚乙二醇干扰素联合利巴韦林治疗,血红蛋白均降至100 g/L。将其按随机数表法随机分为两组,严格组(n=45)和放宽组(n=45),严格组当血红蛋白≤100 g/L时减量,血红蛋白≤80 g/L时停药;放宽组当血红蛋白≤80 g/L时减量,血红蛋白≤60/L时停药。观察两组患者的持续病毒学应答(SVR)、复发率、总胆红素(TBil)、天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、γ-谷氨酰氨基转移酶(γ-GT)等指标和不良反应。结果两组患者在治疗结束停药后随访观察6个月,放宽组患者的SVR明显高于严格组,复发率低于严格组,TBil、AST、ALT和γ-GT与严格组患者相比均有所下降,差异均有统计学意义(P0.05)。两组患者在治疗过程中均出现多种不良反应,但两组患者的不良反应种类及发生率比较差异无统计学意义(P0.05)。结论对于采用聚乙二醇干扰素联合利巴韦林治疗的慢性丙型肝炎患者,放宽利巴韦林剂量调整可以提高患者的SVR,降低患者的复发率,患者的肝脏指标有所改善,并未增加患者的不良反应,值得在临床推广应用。     

聚乙二醇沉淀法与凝胶色谱层析法检测巨泌乳素的相关性研究

目的探讨聚乙二醇（PEG）沉淀法检测巨泌乳素（MPRL）方法的可行性。方法将26例高泌乳素血症（HPRL）患者按临床表现和影像学资料分为真性HPRL组（简称真泌组）11例及MPRL血症组（简称巨泌组）15例。采用凝胶色谱层析（GFC）法分离泌乳素（PRL）各组分,检测各组分PRL浓度,同时将所有标本进行PEG沉淀,检测处理后单体PRL浓度,并与GFC法单体PRL检测结果进行相关性分析。结果巨泌组与真泌组的PRL层析谱不同,真泌组以单体PRL为主,而巨泌组以巨PRL为主。真泌组与巨泌组血清处理前总PRL浓度差异无统计学意义（P〉0．05）,而处理后真泌组明显高于巨泌组（P〈0．05）。PEG沉淀法与GFC法检测真泌组与巨泌组单体PRL浓度差异无统计学意义（P〉0．05）,且相关性良好[相关系数（r）=0．844,P〈0．05]。结论PEG沉淀法检测MPRL结果与GFC法有良好的相关性,且经济、简便、易行,可作为临床常规筛查方法。     

运动及运动强度对荷瘤小鼠移植肿瘤生长的影响

目的:通过不同运动强度的游泳运动,探讨运动及运动强度对小鼠接种肝癌H22和黑色素瘤B16-F10移植瘤生长的影响及机制。方法:将小鼠随机分为正常对照组、荷瘤对照组、持续有氧组、持续力竭组、荷瘤后有氧组、荷瘤后力竭组6组:其中正常对照组不荷瘤,不采取其他实验措施;所有荷瘤组小鼠均在实验开始1周后接种肿瘤细胞:ICR小鼠接种小鼠肝癌细胞H22,C57BL/6小鼠接种小鼠黑色素瘤细胞B16-F10;荷瘤对照组小鼠接瘤建模前后不采取其他实验措施;持续有氧组小鼠每天进行有氧游泳训练,时间从30min逐渐延长至60min,荷瘤后有氧组小鼠从接瘤以后开始进行上述有氧训练;持续力竭组小鼠进行每天负重游泳至力竭的训练,荷瘤后力竭组小鼠从接瘤以后开始进行上述力竭训练。所有组小鼠21d后安乐死,测定其体重、瘤重、胸腺重、脾脏重,计算胸腺指数、脾指数,并检测小鼠脾淋巴细胞增殖能力和脾NK细胞杀伤能力。结果:荷瘤后有氧组ICR小鼠瘤重明显降低(P0.05),C57BL/6小鼠瘤重没有明显变化,荷瘤后有氧组ICR和C57BL/6小鼠脾指数升高(P0.001,P0.01),荷瘤后有氧组ICR小鼠脾淋巴细胞增殖能力和脾NK细胞杀伤能力均明显提高(P0.01,P0.001),而且ICR小鼠荷瘤后有氧组胸腺指数显著升高(P0.05),荷瘤后有氧组C57BL/6小鼠胸腺指数没有明显变化;持续力竭组C57BL/6小鼠瘤重显著增加(P0.05),ICR小鼠瘤重没有明显变化,持续力竭组C57BL/6小鼠脾淋巴细胞增殖能力和脾NK细胞杀伤能力均明显下降(P0.05,P0.001),而持续力竭组ICR小鼠脾淋巴细胞增殖能力虽有下降趋势,但与脾NK细胞杀伤能力一样,与荷瘤对照组相比无显著性差异,胸腺指数却显著升高(P0.01)。结论:在实验时间范围内,运动及运动强度对小鼠肿瘤的生长有明显影响,瘤重的变化与运动强度对小鼠脾脏和胸腺功能的影响相关。     

二氧化锆全瓷冠与金合金烤瓷冠在上前牙牙体缺损修复中的效果及安全性比较

目的 研究分析二氧化锆全瓷冠与金合金烤瓷冠在上前牙牙体缺损修复中的效果及安全性.方法 前瞻性选取2017年7月至2019年5月在聊城市人民医院口腔科进行牙体缺损修复患者124例作为研究对象,按随机数字表法分为对照组和观察组,各62例.对照组给予金合金烤瓷冠进行修复,观察组则给予二氧化锆全瓷冠修复.采用改良美国公共卫生署...     

认知行为疗法对心脏神经症患者康复治疗的作用

目的评价心脏神经症康复治疗中认知行为疗法的作用。方法对60例患者以性别、年龄、诊断相匹配的方法分为两组:对照组30例接受药物治疗,研究组30例接受药物同时合并认知行为疗法治疗。结果治疗后研究组焦虑量表(HAMA)、抑郁量表(HAMD)评分较对照组明显降低(P<0.01),研究组临床疗效明显优于对照组(P<0.05)。结论采用药物合并认知行为疗法比单独使用药物治疗治疗心脏神经症疗效明显。     

静脉推注和静脉滴注葡萄糖酸钙不良反应比较及护理

目的探讨静脉推注和静脉滴注葡萄糖酸钙不同给药方法不良反应发生情况。方法对400例静脉应用葡萄糖酸钙病人,按就诊先后顺序随机分成两组,每组200例,静脉推注葡萄糖酸钙为静注组,静脉滴注葡萄糖酸钙为静滴组,观察病人头晕、头痛、恶心呕吐、胸闷、心前区不适、心律失常等不良反应发生情况。结果静滴组病人头晕、头痛、恶心呕吐、胸闷、心前区不适、心律失常等不良反应发生率明显少于静注组(P<0.05或P<0.01)。结论静脉滴注葡萄糖酸钙较静脉推注葡萄糖酸钙,在减少病人用药不良反应方面有显著的效果,且临床应用简便,值得推广。     

Carrier and dose effects on the pharmacokinetics of T-0128, a camptothecin analogue-carboxymethyl dextran conjugate, in non-tumor- and tumor-bearing rats.

T-0128 is a novel camptothecin (CPT) analogue (T-2513: 7-ethyl-10-aminopropyloxy-CPT)-carboxymethyl (CM) dextran conjugate via a Gly-Gly-Gly linker, with a molecular weight (MW) of 130 kDa. Our previous studies demonstrated that T-0128 has strong antitumor activity against human tumor xenografts that are highly refractory to CPT analogues attributable to the passive tumor targeting of released T-2513. This study examines the effects of carrier, dose, and tumor on T-0128 pharmacokinetics. To study carrier effect, tumor-bearing rats received one i.v. injection of fluorescein isothiocyanate (FITC)-labeled CM dextran with a different degree of substitution (DS) of the carboxymethylated groups and a different MW. Results showed that CM dextran from Dextran T-110 (MW 110 kDa) with a DS value of 0.4 is an appropriate drug carrier for T-0128 regarding plasma half-life and passive tumor targeting. To study dose and tumor effects, non-tumor- and tumor-bearing rats were treated with T-0128 doses ranging from 1 to 25 mg/kg (based on the amount of T-2513 bound to CM dextran). Dose-dependent pharmacokinetics of T-0128 were observed in both kinds of rats. The presence of tumor reduced the plasma half-life and systemic exposure of T-0128. The saturation of hepatic and splenic tissue uptake clearances (CLups), and a large contribution of the tumor CLup to the total body clearance explain these results. Overall, our data provide a rationale for the selection of the carrier for T-0128 and a need for pharmacokinetic studies to evaluate the influences of tumor on the drug disposition.     

神经母细胞瘤原位荷瘤与转移瘤裸鼠模型建立的实验研究

目的用自建的细胞系QDDQ建立起原位荷瘤与转移瘤裸鼠模型,探讨神经母细胞瘤(Neuroblastoma,NB)的转移特性。方法取NB新鲜瘤组织,利用酶消化法,建立起体外细胞系,以(3～7)×107/ml浓度的单细胞悬液0.5 ml接种于裸鼠,建立荷瘤鼠模型。结果 NB原位荷瘤与转移瘤裸鼠模型成功建立,实验鼠共30只,致瘤25只,致瘤率为83.3%。10只原位荷瘤,15只转移荷瘤。结论建立NB细胞动物模型,对于进一步研究其转移特性、分子生物学特征,提供了良好的平台。     

高压氧对缺血再灌注损伤小鼠脑区甘丙肽含量的影响

目的观察小鼠缺血再灌注损伤后各脑区甘丙肽（Gal）含量的变化以及高压氧（HBO）治疗对其含量的影响。方法昆明小鼠24只，随机分为缺血再灌注＋高压氧（HBO）组（A组）、缺血再灌注组（B组），假手术组（C组）和正常对照组（D组），每组6只。A、B组动物采用阻断双侧颈总动脉血流30min后恢复血流的方法建立脑缺血再灌注模型。采用放射免疫分析法测定各组动物脑区Gal免疫活性物质（ir-Gal）含量的变化。结果小鼠缺血再灌注损伤后各脑区Gal含量均有不同程度的变化，皮质ir-Gal含量明显升高，海马ir-Gal含量却明显下降，与C组和D组比较差异均有统计学意义；纹状体ir-Gal含量与D组接近（P〉0．05）。HBO处理后，海马ir-Gal含量明显提高并接近正常水平，皮质与纹状体Gal含量在HBO处理后也明显高于B组和C组，差异均有统计学意义。结论Gal参与了缺血再灌注损伤的病理生理过程，不同脑区的Gal在缺血再灌注损伤中的重要性及作用可能有所不同。HBO处理后各脑区Gal含量明显提高，可能是减少缺血再灌注损伤的作用机制之一。     

磁性5-氟尿嘧啶修饰物在裸鼠大肠癌组织分布的意义

目的探讨聚乙二醇(PEG)修饰的5-氟尿嘧啶磁性白蛋白微球(PEG-5-Fu-MAMS)和5-氟尿嘧啶磁性白蛋白微球(5-Fu-MAMS)对大肠癌组织的被动靶向性。 方法20只人大肠癌裸鼠随机数字表法分为PEG-5-Fu-MAMS组、5-Fu-MAMS组,每组10只,分别将2种不同的制剂(按8mg/kg5-Fu)经尾静脉给药,在磁场下30min后,经眼眶采血,处死大鼠,用高效液相色谱法测定肿瘤组织、血清和肾脏组织中5-Fu的水平。采用SPSS 13.0软件对资料进行统计分析,两组不同组织中5-Fu水平以±s记录,显著性检验采用t检验。 结果注射PEG-5-Fu-MAMS组大肠癌组织中的5-Fu水平为(51.21±2.12)μg/mL,明显高于5-Fu-MAMS组的(33.07±8.21)μg/mL(t＝78.69,P<0.05);而在注射PEG-5-Fu- MAMS组的血清中5-Fu的水平为(1.69±0.53)μg/mL,则明显低于5-Fu-MAMS组的(6.78±0.23)μg/mL(t＝18.76,P<0.05);在肾脏中PEG-5-Fu-MAMS组药5-Fu水平为(21.1±2.3)μg/mL,明显低于5-Fu-MAMS组的(38.2±4.9)μg/mL(t＝39.23,P<0.05)。 结论PEG-5-Fu-MAMS比5-Fu-MAMS的亲大肠癌作用强,PEG-5-Fu-MAMS有望成为一种新的靶向治疗大肠癌的药物。     

Synergistic effects of low‐level laser and mesenchymal stem cells on functional recovery in rats with crushed sciatic nerves

Transplantation of mesenchymal stem cells (MSCs) has been proposed to exert beneficial effects on peripheral nerve regeneration after a peripheral nerve injury, but the functional recovery in the denervated limb is still limited. In this study, we used low‐level laser therapy (LLLT) as an adjunct therapy for MSC transplantation on the functional recovery of crushed sciatic nerve in rats. Peripheral nerve injury was induced in 48 Sprague–Dawley rats by crushing the unilateral sciatic nerve, using a vessel clamp. The animals with crushed injury were randomly divided into four groups: control group, with no treatment; MSC group, treated with MSC alone; LLLT group, treated with LLLT alone; and MSCLLLT group, treated with a combination of MSC and LLLT. The sciatic function index (SFI), vertical activity of locomotion (VA) and ankle angle (AA) of rats were examined for functional assessments after treatment. Electrophysiological, morphological and S100 immunohistochemical studies were also conducted. The MSCLLLT group showed a greater recovery in SFI, VA and AA, with significant difference from MSC, LLLT and control groups (p < 0.05). Moreover, markedly enhanced electrophysiological function and expression of S100 immunoreactivity, as well as fewer inflammatory cells and less vacuole formation were also demonstrated after nerve crush injury in the MSCLLLT group when compared with the groups receiving a single treatment (p < 0.05). MSC transplantation combined with LLLT could achieve better results in functional recovery than a conventional treatment of MSC or LLLT alone. LLLT has a synergistic effect in providing greater functional recovery with MSC transplantation after nerve crush injury. Copyright © 2013 John Wiley & Sons, Ltd.     

音乐干预对心脏介入术围手术期患者生理及焦虑情绪的影响

目的:研究音乐干预对心脏介入术围手术期患者焦虑情绪和生理指标的影响。方法:选取我院心内科接受心脏介入治疗患者60例,按手术时间顺序分成实验组和对照组各30例。心脏介入术前、术中,实验组在接受常规护理方法的基础上加用音乐干预;对照组则仅采用常规护理方法。观察和评价两组患者在心脏介入术前、术后各项生理指标(心率、呼吸、血压)和焦虑情绪。结果:心脏介入术前,实验组各项生理指标和焦虑情绪水平与对照组相比,差异无统计学意义(P>0.05);心脏介入术后,实验组心率、呼吸、收缩压和SAS量表评分较对照组降低,差异有统计学意义(P<0.05)。结论:音乐干预能稳定患者心脏介入术围手术期生理指标,降低患者焦虑情绪水平,提高临床护理质量。     

超声引导下复方倍他米松和盐酸利多卡因注射对脑卒中后肩关节疼痛的改善作用

目的  分析超声引导下复方倍他米松和盐酸利多卡因注射对脑卒中后肩关节疼痛的改善作用。方法  选取2020年1月~ 2021年6月于本院治疗的60例脑卒中后肩关节疼痛患者为研究对象,按照随机数字表法将患者分为治疗组和对照组,30例/ 组。对照组在常规康复治疗基础上进行徒手定位药物注射治疗;治疗组在常规康复治疗基础上进行超声引导下复方倍他米松和盐酸利多卡因注射。观察比较两组患者治疗前后疼痛程度、肩关节活动度、上肢功能运动情况和不良反应发生情况。结果  治疗2周、4周后,治疗组患者视觉模拟评分低于对照组患者（P < 0.05）,肩关节前屈、外展、外旋活动度大于对照组患者（P < 0.05）,Fugl-Meyer运动功能量表上肢部分评分高于对照组患者（P < 0.05）;两组患者不良反应总发生率差异无统计学意义（P>0.05）。结论  相较于徒手定位药物注射治疗,超声引导下复方倍他米松和盐酸利多卡因注射对脑卒中后肩关节疼痛患者疼痛改善效果更为明显,可有效改善肩关节活动度和上肢运动功能。     

维医沙疗皮肤组织的热效应分析

目的探讨维医沙疗的生物组织热效应。方法采用2700型多通道数据采集仪,采集沙疗过程中人体体表温度的动态变化数据,应用皮肤表层组织温度响应方程,计算人体皮肤浅层组织在不同时间段及温度场的热效应状况。结果每年6月中旬至8月中旬,下午15∶00～18∶00时沙疗所气温为(40±2.3)℃,离沙丘表面10～20cm深处沙温可保持在(38.0±4.6)℃。沙疗过程中人体皮肤表层在43℃热沙作用下皮肤表面温度迅速升高(达39℃以上),由于汗液蒸发散热(释热)等,从皮肤表面到皮肤浅层组织的温度逐渐下降,皮肤浅层(X=2.08mm)的温度是(36.0±0.3)℃。2～3h后,皮表温度降至(36.0±0.6)℃,并保持基本不变。结论43℃热沙对皮肤组织不会产生损伤,人体与沙体逐渐达到热平衡,是较理想的沙温;维医沙疗较佳条件为:每年6月中旬至8月中旬,每天下午15∶00～18∶00时,气温在(40.0±2.3)℃,离沙丘表面10～20cm处,沙温保持在(38.0±4.6)℃,每次埋沙0.5～1.0h。     

Antibody‐functionalized nanoparticles for imaging cancer: influence of conjugation to gold nanoparticles on the biodistribution of 89Zr‐labeled cetuximab in mice

Antibody‐labeled gold nanoparticles represent a promising novel tool regarding cancer imaging and therapy. Nevertheless, the characterization of biodistribution of such immunonanocarriers has been poorly documented. In this study, the biodistribution of ⁸⁹Zr‐labeled cetuximab before and after the coupling reaction to gold nanoparticles (AuNPs) was compared and the quantitative imaging performance of ⁸⁹Zr immuno‐PET was evaluated. Cetuximab was functionalized with the desferal moiety and labeled with ⁸⁹Zr (⁸⁹Zr–Df–Bz–NCS–cetuximab). AuNPs with a mean diameter of 5 nm were synthesized according a new method developed in the laboratory, and conjugated to ⁸⁹Zr–Df–Bz–NCS–cetuximab using carbodiimide chemistry (AuNPs–PPAA–cetuximab–⁸⁹Zr). The two tracers were injected in A431 xenograft‐bearing mice. Tumor and liver uptakes were assessed at different times after injection using quantitative PET imaging. The in vivo specificity of the binding was investigated using a saturating dose of unlabeled cetuximab. Radiolabeled cetuximab was conjugated to AuNPs with a coupling reaction yield >75%. All conjugates were stable in vitro and to a lesser extent in plasma. In vivo distribution studies revealed no significant difference in tumor uptake for cetuximab conjugated to nanoparticles up to 72 h after injection, compared with unconjugated cetuximab. Immuno‐PET studies showed that AuNPs–PPAA–cetuximab–⁸⁹Zr provided high tumor‐to‐background ratio. The liver uptake of AuNPs–PPAA–cetuximab–⁸⁹Zr was higher, compared with ⁸⁹Zr–Df–Bz–NCS–cetuximab. In vivo blocking experiments demonstrated selective tumor targeting after coupling reaction. This study showed that the conjugation of AuNPs to cetuximab did not affect its tumor accumulation and that the efficacy of EGFR‐targeted nanoparticles was unaltered. The ⁸⁹Zr‐labeled cetuximab‐targeted gold nanoparticles could be a valuable tool for theranostic purposes. Copyright © 2013 John Wiley & Sons, Ltd.     

苯乙酸联合5-氟尿嘧啶、顺铂对喉癌细胞株凋亡的影响及在荷瘤鼠动物模型中的作用观察

目的探讨苯乙酸（PA）联合5-氟尿嘧啶（5-fu）、顺铂（CDDP）对喉癌细胞株凋亡的影响及在荷瘤鼠动物模型中的联合用药治疗。方法采用苯乙酸联合应用5-Fu、CDDP作用于喉癌细胞,应用流式细胞仪、荷瘤鼠模型等方法,观察联合用药组与单一药物组对喉癌细胞凋亡作用,并制作荷瘤鼠模型进行动物体内实验。结果苯乙酸和5-Fu、CDDP化疗药物联合应用可以明显增强对喉癌细胞株（Hep-2）细胞生长增殖抑制作用,促进细胞形态转化,显著提高促凋亡作用。荷瘤鼠实验模型的结果表明,联合用药可以减少化疗药物剂量及减少耐药性,减轻毒副作用及并发症的发生,为喉癌临床治疗提供了理论基础。