子宫肌瘤中IGF-1受体的变化及其与雌、孕激素受体的关系

目的:探讨人子宫肌瘤及周围正常子宫平滑肌细胞中胰岛素样生长因子受体(IGF-1R)的变化及其与雌、孕激素受体的关系。方法:免疫组织化学SABC法检测雌激素受体(ER)、孕激素受体(PR)、胰岛素样生长因子受体(IGF-1R)的表达,并对其进行图像分析。结果:子宫肌瘤细胞中ER、PR、IGF-1R的表达明显高于子宫肌层细胞中的表达。结论:子宫肌瘤的生长与子宫肌瘤组织ER、PR、IGF-1R的表达增强有关。     

胰岛素受体底物-1在子宫内膜癌中的表达

目的:探讨胰岛素受体底物-1在子宫内膜癌中的表达及其与子宫内膜癌发生的关系。方法:采用免疫组织化学SP法分别对子宫内膜癌、子宫内膜非典型增生、子宫内膜单纯增生和分泌期子宫内膜各30例进行胰岛素样生长因子-1受体、胰岛素受体底物-1、雌激素受体的表达进行检测,同时检测各组细胞的Ki-67指数。结果:胰岛素受体底物-1在4种子宫内膜组织中均有表达,但在子宫内膜癌中表达增强,与分泌期子宫内膜的表达相比差异显著,胰岛素受体底物-1在子宫内膜组织中的表达定位在胞浆;胰岛素样生长因子-1受体定位在胞膜,其在子宫内膜癌中的表达减弱,与分泌期子宫内膜相比差异显著;ER与IGF-1R和IRS-1在4种不同子宫内膜组织中的表达无统计学意义;Ki-67指数在子宫内膜癌及癌前病变与子宫内膜良性病变及正常子宫内膜间相比较统计学差异显著;Ki-67指数与IRS-1间存在正相关。结论:胰岛素受体底物-1在子宫内膜癌的发生、发展中可能起着重要的调节作用。     

胰岛素样生长因子系统与卵巢癌

胰岛素样生长因子(IGF)系统存在于正常卵巢中,在正常和肿瘤细胞生物学中起重要作用。综述近年来国内外在IGF系统及其在卵巢癌发生发展及耐药等方面的研究,发现卵巢癌患者血清及组织中IGF-1及IGF-1R、IGFBP-2水平增高,而IGFBP-3水平降低。IGFBP-2水平在上皮性卵巢癌患者血清中增高并与CA125正相关,有望成为卵巢癌潜在的标志物。使用反义寡核苷酸技术在基因水平上高度选择性地抑制IGF-1、IGF-2或IGF受体的表达均可有效抑制肿瘤的生长,显示抗IGF-1受体的反义策略可提供治疗卵巢癌的新方法。胰岛素样生长因子系统与卵巢癌的关系值得进一步研究。     

胰岛素样生长因子系统与卵巢癌

胰岛素样生长因子(IGF)系统存在于正常卵巢中,在正常和肿瘤细胞生物学中起重要作用.综述近年来国内外在IGF系统及其在卵巢癌发生发展及耐药等方面的研究,发现卵巢癌患者血清及组织中IGF-1及IGF-1R、IGFBP-2水平增高,而IGFBP-3水平降低.IGFBP-2水平在上皮性卵巢癌患者血清中增高,并与CA125正相关,有望成为卵巢癌潜在的标志物.使用反义寡核苷酸技术在基因水平上高度选择性地抑制IGF-1、IGF-2或IGF受体的表达均可有效抑制肿瘤的生长,显示抗IGF-1受体的反义策略可提供治疗卵巢癌的新方法.胰岛素样生长因子系统与卵巢癌的关系值得进一步研究.     

IGF-2、IGF-1R、IGF-2R在子宫内膜腺癌的表达及临床意义

目的:检测Ⅰ型子宫内膜癌中IGF-2及其相应受体(IGF-1R和IGF-2R/M6P)蛋白的表达情况,探讨IGF-2及其相应受体在Ⅰ型子宫内膜癌变中的表达及作用。方法:采用免疫组化PV法检测正常增生期子宫内膜(对照组)20例、子宫内膜腺癌(腺癌组)30例组织中IGF-2、IGF-1R和IGF-2R的表达情况。结果:IGF-2、IGF-1R的过阳性表达在Ⅰ型子宫内膜癌显著高于正常子宫内膜(P=0.007,P=0.006)。IGF-2R的阳性表达与过阳性表达在子宫内膜癌细胞均低于正常子宫内膜细胞(P=0.043,P=0.000)。结论:IGF-2R阳性表达的下调与IGF-2及IGF-1R的异常高表达在Ⅰ型子宫内膜癌的发生发展过程中起重要作用。     

胰岛素样生长因子系统与卵巢癌

胰岛素样生长因子(IGF)系统存在于正常卵巢中，在正常和肿瘤细胞生物学中起重要作用。综述近年来国内外在IGF系统及其在卵巢癌发生发展及耐药等方面的研究，发现卵巢癌患者血清及组织中IGF-1及IGF-1R、IGFBP-2水平增高，而IGFBP-3水平降低。IGFBP-2水平在上皮性卵巢癌患者血清中增高，并与CA125正相关，有望成为卵巢癌潜在的标志物。使用反义寡核苷酸技术在基因水平上高度选择性地抑制IGF-1、IGF-2或IGF受体的表达均可有效抑制肿瘤的生长，显示抗IGF-1受体的反义策略可提供治疗卵巢癌的新方法。胰岛素样生长因子系统与卵巢癌的关系值得进一步研究。     

左炔诺孕酮宫内缓释系统对增生过长子宫内膜IGF-Ⅰ和IGF-Ⅱ及其受体表达的影响

目的:探讨左炔诺孕酮宫内缓释系统(LNG-IUS)对增生过长子宫内膜胰岛素样生长因子-Ⅰ(IGF-Ⅰ)和胰岛素样生长因子-Ⅱ(IGF-Ⅱ)及其相应受体(IGF-IR,IGF-ⅡR)表达的影响。方法:应用免疫组化链霉亲和素-生物素-过氧化物酶复合物(SABC)方法,分别检测子宫内膜增生过长患者子宫内置入LNG-IUS(LNG-IUS组)及炔诺酮(炔诺酮组)治疗前后子宫内膜腺上皮细胞及间质细胞中IGF-Ⅰ和IGF-Ⅱ及其相应受体的表达,并与正常增生期、分泌期子宫内膜中相关因子表达作比较。结果:宫内置入LNG-IUS3个月后,IGF-Ⅰ表达下降,IGF-Ⅱ的表达上升,与治疗前比较有统计学意义(P<0.05)。LNG-IUS组子宫内膜IGF-Ⅰ表达明显低于炔诺酮组,两者比较有统计学意义(P<0.05);IGF-Ⅱ在LNG-IUS治疗后的子宫内膜的表达高于炔诺酮治疗后的子宫内膜,但无统计学意义。LNG-IUS治疗前后IGF-IR表达无显著性变化,各组之间的比较无统计学意义(P>0.05)。宫内置入LNG-IUS3个月后,IGF-IIR表达增强,治疗前后比较差异有统计学意义(P<0.05)。结论:LNG-IUS通过抑制增生过长子宫内膜IGF-Ⅰ表达,上调IGF-Ⅱ表达,抑制雌激素对子宫内膜的增生效应。LNG-IUS对子宫内膜IGF系统的调节作用较口服炔诺酮显著。子宫内膜增生过长宫腔内置入LNG-IUS后子宫内膜IGF系统的表达与增生期、分泌期子宫内膜均不相同,呈现一种极弱IGF-Ⅰ的表达和强IGF-Ⅱ表达类型。     

胰岛素样生长因子Ⅰ、Ⅱ与子宫内膜异位症关系的研究

目的:探讨胰岛素样生长因子Ⅰ、Ⅱ(IGF-Ⅰ、IGF-Ⅱ)在子宫内膜异位症发病机制中所起的作用。方法:用免疫组织化学方法检测30例子宫内膜异位症患者在位子宫内膜、异位子宫内膜及20例正常子宫内膜IGF-Ⅰ、IGF-Ⅱ的表达。结果:子宫内膜异位症患者异位内膜组织中IGF-Ⅰ表达水平高于在位子宫内膜(P<0.05)及正常子宫内膜(P<0.01);在位内膜中IGF-Ⅰ的表达高于正常子宫内膜,但无统计学意义(P>0.05)。异位内膜上未见IGF-Ⅱ表达,但正常子宫内膜IGF-Ⅱ表达高于在位内膜(P<0.05)。结论:IGF-Ⅰ、IGF一Ⅱ的异常表达在子宫内膜异位症发病过程中起一定作用。     

胃腺癌组织中IGF-1R和VEGF的表达及其临床意义

目的探讨胃腺癌组织中胰岛素样生长因子-1受体(IGF-1R)和血管内皮生长因子(VEGF)的表达及其临床意义,分析二者的相关性。方法应用免疫组化SP技术,对56例胃腺癌组织、40例癌旁不典型增生组织和56例正常胃组织中IGF-1R和VEGF的表达情况进行检测分析。结果胃腺癌组织、癌旁不典型增生组织中IGF-1R和VEGF的阳性表达率显著高于正常胃组织(P<0.05);有淋巴结转移的胃癌组织中IGF-1R和VEGF的阳性表达率显著高于无淋巴结转移组(P<0.05);IGF-1R和VEGF的阳性表达率随胃癌浸润深度的增加而增高(P<0.05);IGF-1R与VEGF的表达呈正相关(P<0.01)。结论IGF-1R和VEGF在胃腺癌组织中的高表达与胃腺癌的发生、发展、浸润及转移有关;二者可能在胃腺癌的发生发展中起了协同作用。     

B族链球菌性化脓性脑膜炎患儿血清和脑脊液胰岛素样生长因子的表达

目的探讨B族链球菌感染所致脑膜炎患儿血清和脑脊液胰岛素样生长因子的表达水平变化。方法选取2014年1月1日-2019年2月28日江西省新余市妇幼保健院新生儿科44例B族链球菌感染的化脓性脑膜炎患儿为研究组,包括急性期和恢复期;正常新生儿23名为对照组,放射免疫分析法(Radioimmunoassay,RIA)检测入组研究对象血清和脑脊液中胰岛素样生长因子1(Insulin-like growth factor 1,IGF-1)和胰岛素样生长因子2(Insulin-like growth factor 2,IGF-2)的表达水平。结果研究组急性期血清IGF-1和IGF-2表达水平分别为(4.4±2.4)mg/L和(20.53±2.42) mg/L均低于正常对照组的(64.3±16.9)mg/L和(74.36±18.60)mg/L,两组比较差异有统计学意义(P0.05),而脑脊液IGF-1和IGF-2表达水平(18.5±5.6)mg/L和(42.03±5.34)mg/L较正常对照组的(2.8±1.4)mg/L和25.43±1.99)mg/L显著升高(P0.05);与急性期相比,恢复期血清IGF-1和IGF-2表达水平显著升高(P0.05),脑脊液IGF-1和IGF-2表达水平显著降低(P0.05);恢复期血清和脑脊液IGF-1表达水平与正常对照组比较,差异无统计学意义。结论 B族链球菌感染所致化脓性脑膜炎的新生儿的血清中胰岛素样生长因子的表达水平显著降低,脑脊液中胰岛素生长因子的表达水平显著升高。     

Zyflamend Reduces the Expression of Androgen Receptor in a Model of Castrate-Resistant Prostate Cancer

Prostate cancer is the most commonly diagnosed solid malignancy, and tumor cells eventually transform to castrate resistance through multiple pathways including activation of the androgen receptor via insulin-like growth factor receptor (IGF-1R) signaling involving phospho-AKT (pAKT). In this study, a mixture of herbal extracts, Zyflamend®, was used as a treatment in a model of castrate-resistant prostate cancer using CWR22Rv1 cells. Zyflamend reduced androgen receptor and IGF-1R expression along with a reduction of IGF-1-mediated proliferation of CWR22Rv1 cells. IGF-1 induced downstream AKT phosphorylation; however, the induction of pAKT was not associated with androgen receptor expression. Further, constitutively active form of AKT had no effect on nuclear expression of androgen receptor, indicating that upregulation of pAKT did not promote androgen receptor expression or nuclear translocation in castrate-resistant CWR22Rv1 cells. Conversely, Zyflamend reduced androgen receptor expression following IGF-1 stimulation and in cells overexpressing pAKT. These results demonstrated that Zyflamend inhibited IGF-1-stimulated cell growth, IGF-1R expression, and androgen receptor expression and its nuclear localization, but these effects were not dependent upon phosphatidylinositol 3-kinase/pAKT signaling. In conclusion, Zyflamend decreased cell proliferation and inhibited IGF-1R and androgen receptor expression in a phosphatidylinositol 3-kinase/pAKT independent manner.     

Zyflamend reduces the expression of androgen receptor in a model of castrate-resistant prostate cancer

Prostate cancer is the most commonly diagnosed solid malignancy, and tumor cells eventually transform to castrate resistance through multiple pathways including activation of the androgen receptor via insulin-like growth factor receptor (IGF-1R) signaling involving phospho-AKT (pAKT). In this study, a mixture of herbal extracts, Zyflamend?, was used as a treatment in a model of castrate-resistant prostate cancer using CWR22Rv1 cells. Zyflamend reduced androgen receptor and IGF-1R expression along with a reduction of IGF-1-mediated proliferation of CWR22Rv1 cells. IGF-1 induced downstream AKT phosphorylation; however, the induction of pAKT was not associated with androgen receptor expression. Further, constitutively active form of AKT had no effect on nuclear expression of androgen receptor, indicating that upregulation of pAKT did not promote androgen receptor expression or nuclear translocation in castrate-resistant CWR22Rv1 cells. Conversely, Zyflamend reduced androgen receptor expression following IGF-1 stimulation and in cells overexpressing pAKT. These results demonstrated that Zyflamend inhibited IGF-1-stimulated cell growth, IGF-1R expression, and androgen receptor expression and its nuclear localization, but these effects were not dependent upon phosphatidylinositol 3-kinase/pAKT signaling. In conclusion, Zyflamend decreased cell proliferation and inhibited IGF-1R and androgen receptor expression in a phosphatidylinositol 3-kinase/pAKT independent manner.     

An energy-rich diet causes rumen papillae proliferation associated with more IGF type 1 receptors and increased plasma IGF-1 concentrations in young goats

We tested the hypothesis that the dietary energy-dependent alterations of the rumen papillae size are accompanied by corresponding changes in systemic insulin-like growth factor (IGF)-1 concentration and in rumen papillary IGF type 1 receptors (IGF-1R). Young male goats (n=24) were randomly allocated to two groups (n=12) and fed a high level (HL) metabolizable energy [1200 kJ/(kg(0.75).d)] or a low level (LL) [500 kJ/(kg(0.75).d)] diet for 42 d. The concentration of ruminal total SCFA did not differ between the groups, but the molar proportion of butyric acid was enhanced by 70% in the HL group (P<0.05). Both the length and width of the papillae were greater (P<0.05) in the HL group, and the surface was 50-100% larger (P<0.05) in the tissue sampled from the artrium ruminis, the ventral ruminal sac and the ventral blind sac. Transport of Na+ across the rumen epithelium, which is amiloride sensitive, was higher (P<0.05) in the HL than in the LL group. Furthermore, the plasma IGF-1 concentration was about twofold higher in the HL group (P<0.05), and the maximal rumen epithelial IGF-1R binding was also higher in the HL (P<0.05) than in the LL group. IGF-1R mRNA and IGF-1 mRNA were detected in rumen papillae; however, they were unaffected by dietary treatments. DNA synthesis and cell proliferation of cultured rumen epithelial cells were higher (P<0.05) after IGF-1 treatment (25 or 50 microg/L) compared with those in the medium without IGF-1. Thus dietary energy-dependent alterations of rumen morphology and function are accompanied by corresponding changes in systemic IGF-1 and ruminal IGF-1R.     

子宫内膜癌创伤后成长水平及影响因素分析

目的 探讨子宫内膜癌创伤后成长水平及影响因素,以期为提高该类患者创伤后成长水平提供指导依据.方法 选取2017年9月至2019年11月贺州市人民医院收治的子宫内膜癌患者168例作为研究对象,对其一般资料、领悟社会支持、创伤后成长情况进行调查统计.使用单因素和多重线性回归分析其中影响因素.结果 患者的创伤后成长水平总分为...