母婴 ABO血型不合合并 IgG抗-M抗体引起新生儿溶血病 1例

目的 分析ABO血型不合合并低效价免疫球蛋白G(IgG)抗-M抗体引起的新生儿溶血病的临床特点.方法 报告ABO血型不合合并低效价IgG抗-M抗体引起的新生儿溶血病(HDN)1例,病例样本为2017年11月河北省血液中心接收的临床病人标本,对病儿及其父母进行血型鉴定,利用抗体鉴定谱细胞检测病儿及其母亲血清、病儿红细胞放散液中存在的抗体,并进行抗体效价测定.结果 母亲与病儿血清及放散液中均含有抗-M抗体且病儿合并ABO-新生儿溶血病,即ABO-HDN,母亲血清在盐水介质测得免疫球蛋白M(IgM)抗-M效价为64;母亲血清经2-巯基乙醇(2-Me)处理后,抗人球蛋白试验测得IgG抗-M效价为4.结论 IgG抗-M抗体引起的新生儿溶血病极其少见,但极易造成严重的新生儿溶血病,在临床产前筛查时应给予重视.     

链球菌溶血素“O”抗体测定的临床意义

一、什么是链球菌溶血素“O”抗体?链球菌“O”溶血素是 A 族溶血性链球菌的代谢产物之一,能溶解红细胞。“O”溶血素具有很强的抗原性,人受溶血性链球菌感染后能产生针对“O”溶血素的抗体。测定该抗体(俗称抗“O”测定)的存在和其浓度的高     

预免疫b型流感杆菌偶联菌苗对人B淋巴细胞应答的影响

本文报道了预免疫b型流感杆菌(PRP)和白喉类毒素(DT)偶联菌苗对人B淋巴细胞应答的影响,用25μg PRP和18μg DT共价结合的偶联菌苗(PRP-D)对32名成人志愿者接种1或2次。在免疫前(0天)和免疫后4周,收集血样测定抗体浓度,在免疫后的第6、7、8、9天采血测定分泌抗体细胞(AbSC)数,用溶血斑形成法和ELISA法分别测定B细胞的分泌能力和抗体水平,结果如下。健康成人首次接种PRP-D偶联菌苗后4周,血浆中PRP的IgG、IgM和IgA的抗体总量的增长为32.0μg/ml,DT的抗体总量的增长为1.14IU/ml,但其中IgM抗体未增长,同免疫前基本一致。免疫后除1人外在6～9天时PRP和DF的血循AbSC的数目均增加。其中IgA的AbSC的数目占IgG和IgM的一半以上,DT的AbSC主要为IgG。     

五味子宁神口服液的免疫调节作用研究

目的:研究五味子宁神口服液(WOL)的免疫调节作用.方法:采用测定小鼠碳粒廓清率、二硝基氟苯(DNFB)诱导小鼠迟发型超敏反应、血清溶血素抗体IgM水平,以及测定ConA和LPS诱导的脾T、B淋巴细胞的增殖转化实验(MTT法).结果:WOL提高小鼠血清溶血素抗体IgM水平及免疫功能低下小鼠的吞噬指数、吞噬系数、耳肿胀度、脾和胸腺指数,增强ConA和LPS诱导的脾T、B淋巴细胞增殖转化反应.结论:WOL对小鼠具有一定的免疫增强作用.     

MacELISA、RPHI和IFAT应用于EHF早期诊断的比较研究

<正> 流行性出血热(EHF)的早期实验诊断,最理想的方法是从患者标本中直接测特异性抗原或IgM类特异抗体。由于抗原检测需在发病的最初几日之内,且持续存在时间较短,标本难以取得,故测IgM类特异抗体是目前采用较多的方法。本文采用MacELISA(测IgM抗体)和RPHI(测总抗体)对山西省EHF疫区的确诊病例、疑似病例、非出血热病例及正常献血员血清标本进行EHF抗体测定和IFAT(分别检测IgG和IgM特异抗体)进行并了比较,现将结果报告如下: 材料和方法一、血清标本:1985～1988年山西省EHF疫区确诊病例(具有临床症状,IFAT测定双份血清IgG抗体4倍以上增高或单份血清IgG抗体效价≥1:1280者)血清标本76份和临床疑似病例(具有     

一种候选口服伤寒/霍乱杂交活菌苗对人体的免疫原性

本文报道,将稻叶型霍乱弧菌569B株O抗原基因插入到伤寒杆菌减毒菌苗株Ty21a中,制备成口服杂交菌苗.口服免疫10名志愿者,用ELISA测定周围血淋巴细胞(PBL)和血清对菌苗的特异性免疫应答.测定PBL结果表明,10名接种者对伤寒杆菌脂多糖(LPS)产生IgA抗体应答,并观察到IgG(7名)和IgM(10名)应答.血清IgA、IgG和IgM抗伤寒杆菌LPS抗体增加4倍以上者分别为7人、4人和3人.10人中有8人杀菌抗体滴度明显增加     

孕妇IgG抗体效价与新生儿溶血病发病的相关研究

目的：探讨O型血孕妇血清中IgG抗体效价与新生儿溶血病发病率之间的关系。方法采用微柱凝胶检测O型孕妇IgG抗A(B)效价及新生儿溶血三项,分析IgG抗体效价与新生儿溶血病发病率的关系。结果528例O型孕妇IgG抗A(B)效价测定,抗体效价<1：64者占59.5%,抗体效价1：64者占16.7%,抗体效价1：128者占10.6%,抗体效价1：256者占9.5%,抗体效价1：512者占3.8%。ABO-HDN发病率与母体血清IgG抗体效价成正相关,抗体效价超过1：64 HDN发生率明显增加,发病几率为64.5%(138/214)。结论 O型孕妇IgG抗体效价与新生儿发病率成正相关,随孕妇孕期IgG抗体效价增高,新生儿溶血病发病逐渐增高。监测孕期IgG抗体效价可预测新生儿溶血病发病情况,提示医生采取医学干预可减轻其危害。     

孕妇血清抗-A(B)抗体检测对新生儿溶血诊断的价值探析

目的探讨孕妇血清抗-A(B)抗体检测对新生儿溶血诊断的价值。方法选取2015年3月至2016年3月在我院分娩的149例夫妻ABO血型不合的孕妇及其分娩的新生儿,检测孕妇分娩前IgG抗-A(B)抗体,并根据检测结果进行分组,比较各组新生儿溶血的发生率。结果 149例夫妻ABO血型不合孕妇中,IgG抗-A(B)抗体阳性85例,其中,IgG抗-A(B)抗体浓度≥1:64的有64例,占75.29%,发生新生儿溶血的有29个,发病率为45.31%,作为阳性孕妇组;IgG抗-A(B)抗体<1:64的有21例,占24.71%,发生新生儿溶血3例,发病率为14.29%,作为阴性孕妇组,经卡方检验,2组发病率差异有统计学意义(P<0.05);阳性孕妇组中,IgG抗-A(B)抗体滴度≥1:64组新生儿溶血发病率为22.65%,IgG抗-A(B)抗体滴度≥1:128组新生儿溶血发病率为59.62%,IgG抗-A(B)抗体滴度≥1:256组新生儿溶血发病率为75.64%;IgG抗-A(B)抗体滴度≥1:512新生儿溶血发生率为85.59%,结果显示,各组间的新生儿溶血发病率组间比较差异均有统计学意义(P<0.05),且随着IgG抗-A(B)抗体滴度的增加,新生儿溶血的发病率随之增加。结论孕妇分娩前IgG抗-A(B)抗体滴度的升高可显著增加新生儿溶血的发病率,对于夫妻ABO血型不合的孕妇,应加强其IgG抗-A(B)抗体的检测。     

孕期IgG抗体效价与新生儿溶血病的关系分析

目的:探讨O型血孕妇妊娠期血清IgG抗体效价对产后新生儿ABO溶血病(HDN)的发病影响.方法:选择血型为O型RhD阳性、其丈夫血型为非O型的孕妇分娩新生儿457例,孕期进行血清抗A或抗B的IgG抗体测定,效价≥1:64者为阳性,分娩时取脐静脉血做新生儿血型鉴定、血清胆红素、溶血三项试验,根据IgG抗体效价与HDN发生情况进行分析.结果:妊娠次数在2次及2次以上者,其IgG抗A(B)阳性率大于1次妊娠者,差异有显著性(P＜0.05);有不良妊娠史者发生率高,差异有显著性(P＜0.05);随着孕妇血型抗体效价的增高,新生儿溶血病的发生率也逐渐增高(P＜0.05).结论:对妊娠次数较多或有不良妊娠史的孕妇在孕早期应进行IgC航A(B)抗体效价的测定,并对整个孕期动态观察IgG抗体效价的变化.可作为判断胎儿出生后是否发生新生儿溶血病的筛查指标,对降低新生儿母婴血型不合所致溶血病具有积极作用.     

27例带状疱疹患者血清特异性IgA、IgG和IgM抗体的测定

目的对带状疱疹患者的血清特异IgA、IgG和IgM抗体水平进行测定,观察带状疱疹患者的免疫表达情况,分析各抗体水平和疾病的关系。方法选取2011年2月至2013年3月在桂林医学院第二附属医院收治的带状疱疹患者和健康志愿者,各27例,两组均用酶联免疫吸附测定法(ELISA)方法检测IgA、IgG和IgM抗体,比较带状疱疹患者和健康志愿者体内的三种抗体的表达水平的差异。结果IgM和IgG抗体检测结果,在带状疱疹患者和健康志愿者表达无明显差异(P>0.05);IgA抗体水平在带状疱疹患者体内检测结果显著高于健康志愿者(P<0.05)。结论 IgM和IgG抗体不能作为带状疱疹患者病毒检测指标,IgA抗体有可能作为带状疱疹患者病毒检测指标,但是需要更多样本和相关研究进一步进行验证。     

A spectrum of antibody (IgG. IgG1, IgM) response in mice infected with trichinella spiralis treated with L-mimosine

The purpose of this study was to demonstrate the anti-inflammatory effects of L-mimosine on chronic inflammation, by investigating its effect on the immunological response of BALB/c mice infected with the nematode parasite Trichinella spiralis. Specific anti-parasite immunoglobulins (IgG, IgG1 and IgM) were detected by the ELISA method in the serum of both the treated and the untreated animals at different periods of time for 60 days post infection. Two groups consisting of 18 mice each were used. The mice were 6 weeks of age. Both groups were infected with 220 larvae (L1-T. spiralis) per os: one group was administered an intraperitoneal injection of L-mimosine (200 &mgr;g/100 ml/dose) for 27 days (the first injection started 7 days before infection) and the second group was administered an intraperitoneal injection of saline solution (100 &mgr;l/dose). Parasite specific IgG, IgG1 and IgM levels were determined in the sera of infected, untreated mice. The levels of IgG and IgG1 were increased following infection and remained elevated throughout the experimental period, while IgM was significantly decreased on the 50th day post-infection. These levels were found to be lower in the L-mimosine treated infected mice, compared to the untreated mice. The inhibition started from day 10 and continued until day 60. In healthy animals, the production of immunoglobulins was not measurable. Non-infected animals treated with L-mimosine also showed no detectable anti-parasite specific immunoglobulins.     

Vomitoxin-induced dysregulation of serum IgA, IgM and IgG reactive with gut bacterial and self antigens.

The effect of dietary vomitoxin exposure on immunoglobulins that react with naturally occurring gut bacterial and self antigens was assessed in the B6C3F1 mouse. Ingestion of 25 ppm vomitoxin for 4 and 8 wk resulted in significantly elevated total IgA but depressed total IgG and IgM in serum when compared with control mice fed semi-purified diet only. IgA specific for phosphorylcholine (PC) and inulin (haptens associated with intestinal bacteria) increased significantly in mice fed vomitoxin whereas IgM with the identical specificity decreased. When sera were assessed for autoantibodies recognizing DNA and bromelated mouse red blood cells (MRBC), vomitoxin-exposed mice exhibited elevated specific IgA as compared with controls. This occurred together with decreases in DNA-specific IgG and IgM, and decreases in MRBC-specific IgM. Additionally, vomitoxin exposure did not enhance the specific serum IgA response to orally administered trinitrophenylated sheep red blood cells (TNP-SRBC), but significantly depressed TNP-specific serum IgG. The results suggest that hyperelevation of total and specific serum IgA for oral and self antigens occurs during vomitoxin feeding and that may be coupled with down-regulation of total and specific IgM or IgG. These effects could be contributory to the capacity of vomitoxin to induce IgA immune complex glomerulonephritis.     

蜂花粉对小鼠血清IgM和IgG含量的影响

以单向免疫扩散试验观察蜂花粉对小鼠血清IgM和IgG含量的影响,结果表明蜂花粉提高正常小鼠及环磷酰胺、氢化可的松、~(60)Co照射与荷瘤所致免疫低下小鼠的血清IgM和IgG含量。并初步分析了作用机理。     

Effects of the aqueous extract of epimedii herba on the antibody responses in mice

Antibodies and cytokines in serum were detected in male ICR mice treated with the aqueous extract of Epimedii Herba (AEEH) at doses of 40, 120 and 360 mg/kg orally for 2 weeks. Effects of AEEH on antibody forming responses were assessed by enzyme linked immunosorbent assay (ELISA) of immunoglobulin (Ig) levels in serum collected 7 days after priming with ovalbumin (OVA) in complete Freund's adjuvant (CFA) or immediately without priming at week 2. The relative spleen weight was significantly increased by AEEH, compared with controls, especially at a dose of 120 mg/kg of it after priming with OVA and 40 mg/kg without priming, respectively. However, body weight gain was slightly decreased in AEEH-fed mice. The enhancement of total serum IgG and IgG1 levels in unprimed mice was statistically significant in mice fed 40 mg/kg AEEH. Total serum IgG2a levels and Il-4 secretion were also statistically augmented by all groups of AEEH treatment. A tendency to marked increase of total serum IgM level and IFN-gamma secretion was also observed in mice fed 40 and 120 mg/kg AEEH but not those fed 360 mg/kg AEEH. When mice were immunized with OVA, furthermore, a marked stimulation of antibody formation and cytokines secretion was observed in all groups of AEEH-fed mice compared with controls. These findings indicate that AEEH at therapeutic concentrations enhances the production of antibodies and cytokines in mice, and the enhancing effects are more marked when the mice were immunized with OVA. Thus, these results suggest that AEEH is effective on Th cell functions, and protective effects on host against immune diseases.     

N-Acetyl-D-glucosamine-coated polyamidoamine dendrimer modulates antibody formation via natural killer cell activation

N-Acetyl-d-glucosamine-coated polyamidoamine dendrimer (GlcNAc₈) was shown previously to exhibit binding affinity to the rat recombinant NKR-P1 molecule (known in mice also as NK1.1) and to induce NK cell-mediated cytotoxicity. In this study, we investigated whether GlcNAc₈ modulates antibody formation as activated NK cells were reported to participate in its regulation. C57BL/6 mice treated with GlcNAc₈ and intact controls were immunized either with sheep red blood cells (SRBCs), 2,4-dinitrophenylated-lipopolysaccharide (DNP-LPS) or keyhole limpet hemocyanin (KLH) for evaluation of splenic antibody forming cell counts and serum immunoglobulin (Ig) levels. In vitro Ig formation was determined using supernatants of spleen mononuclear cells (SMCs) and CD49b or NK1.1-depleted SMC subpopulations. Serum antigen-specific IgG2a levels were also measured in DBA/2 and BALB/c mice (NK1.1-negative mouse strains on the basis of flow cytometric analysis) which possess different Nkr-p1c gene form than C57BL/6 ones. A significant increase in anti-SRBC IgG forming cells, serum levels of anti-KLH as well as anti-DNP IgG and IgG2a was observed after GlcNAc₈ administration in C57BL/6 mice. IgM levels in supernatants of SMCs stimulated in vitro simultaneously with DNP-LPS and GlcNAc₈ were significantly mounted compared with supernatants of SMCs primed with the antigen alone, but this enhancement was blocked after depletion of CD49b-positive or NK1.1-positive cells. In DBA/2 and BALB/c mice, GlcNAc₈ influenced neither serum levels of anti-KLH nor anti-DNP IgG2a. These results indicate that GlcNAc₈-induced upregulation of antibody formation is triggered by NK cell stimulation and depends on expressed NKR-P1 isoforms, particularly NKR-P1C.     

Murine cytomegalovirus infection model in Balb/c mice. 3. Immunoglobulin production during infection.

In mice infected with a lethal dose of murine cytomegalovirus (MCMV) the serum immunoglobulin (Ig) levels and the Ig-bearing cells in the spleen dropped to barely detectable levels 2 days after infection. In mice with acute but non-lethal MCMV infection, the serum IgM was twice and the IgG 32 times that of the uninfected controls by Day 8 of infection; the numbers of spleen cells bearing IgM and the IgG subclasses (IgG1, IgG2a, IgG2b, IgG3) were also greatly increased. In the asymptomatically infected group, serum IgM remained unchanged but the IgG increased to 16 times that of uninfected controls by Day 11 of infection; the numbers of spleen cells bearing IgM and IgG subclasses were also increased, although to a lesser extent than in the acute, non-lethally infected mice. In the latter two groups, serum IgA and IgA-bearing cells in the spleen did not alter significantly. Complement-requiring neutralizing antibodies to MCMV were detected 8 days post infection.     

疏肝舒乳颗粒抗炎镇痛和免疫作用

目的:研究疏肝舒乳的抗炎、镇痛作用和免疫调节作用.方法:用二甲苯致小鼠耳廓炎症和大鼠棉球肉芽肿实验,观察疏肝舒乳的抗炎作用;用热板和扭体实验观察疏肝舒乳的镇痛作用;以血清溶血素(IgG、IgM)和脾指数为指标,观察疏肝舒乳对小鼠体液免疫功能的影响;以小鼠迟发超敏反应和胸腺指数为指标,观察疏肝舒乳对小鼠细胞免疫功能的影响.结果:疏肝舒乳颗粒能明显抑制小鼠耳肿胀、大量肉芽肿、降低小鼠扭体次数和降低小鼠脾脏指数;疏肝舒乳颗粒还能明显降低半素血清溶血素(HCIgM、HCIgG)含量和抑制致敏红细胞(SRBC)引起的小鼠迟发超敏反应.结论:疏肝舒乳颗粒具有抗炎、镇痛以及免疫抑制作用.     

Experimental Murine IgA Nephropathy following Passive Administration of Vomitoxin-induced IgA Monoclonal Antibodies

Oral exposure of mice to vomitoxin (VT) induces elevated levels of serum IgA, circulating IgA immune complexes (IgA-IC), mesangial IgA deposition and haematuria, which all mimic the clinical signs of human IgA nephropathy (IgAN). To further assess the effects of VT-induced IgA in the murine model, B6C3F₁ and BALB/C mice were injected intraperitoneally with affinity-purified monoclonal IgA derived from Peyer's patch hybridomas of VT-exposed mice. In B6C3F₁ mice, serum IgA, IgM and IgA-IC levels were increased two- to fivefold in treatment groups after 4 and 6 wk compared with controls, whereas increases in serum IgG as high as 18-fold were observed. Urinary erythrocyte counts were also significantly elevated in treatment groups after 2, 4 and 6 wk compared with controls. Concurrent increases in IgA and IgG complexes containing casein, the dietary protein source, occurred in treatment mice. Mesangial IgA, IgG, IgM and C3 deposition were significantly increased in all treatment mice after 6 wk. Electron-dense deposits occurred in the glomeruli of IgA-injected mice after 6 wk. All the above parameters were similarly affected in BALB/C mice. Injection of IgA-secreting hybridoma cells into BALB/C mice increased serum IgA, IgA-IC and IgG levels as well as elevated mesangial IgA, IgG and C3 deposition and haematuria after 2–3 weeks compared with controls. In total, these data indicate that passive administration of VT-induced IgAs can induce the hallmarks of IgA nephropathy. Casein, an antigen found in the diet used for these mice, appeared to form IC with IgA or IgG and these IC may participate in the pathogenesis of this nephropathy.     

豹皮樟总黄酮对免疫低下小鼠免疫功能的影响

目的研究豹皮樟总黄酮(litsea coteana total flavone,LCTF)对环磷酰胺(cyclophosphamide,Cy)诱导的免疫低下小鼠免疫功能的影响。方法以Cy50mg·kg-1ip给药,每天1次,连续2d,诱导小鼠免疫低下模型,采用碳廓清试验、溶血素生成试验、二硝基氟苯(DNFB)诱导的迟发性超敏反应,观察LCTF对免疫低下小鼠非特异性免疫、体液免疫和细胞免疫的影响。结果碳廓清试验,LCTF(200、400mg·kg-1)组可提高Cy致免疫低下小鼠的吞噬指数α值和廓清指数K值,提高免疫低下小鼠巨噬细胞的吞噬功能;溶血素试验,LCTF(100、200mg·kg-1)组能提高免疫低下小鼠血清IgM和IgG的生成,LCTF(100、200、400mg·kg-1)能增加免疫低下小鼠脾细胞溶血素的产生;LCTF(200、400mg·kg-1)组可明显促进免疫低下小鼠DTH反应,LCTF(100、200、400mg·kg-1)能提升CD4+、CD8+细胞数以及CD4+/CD8+比值,并能提高脾淋巴细胞生产IL-2含量。结论以上结果表明,LCTF通过广泛刺激小鼠的特异性及非特异性免疫应答,对免疫低下小鼠的免疫功能有良好的增强作用。     

枇杷叶三萜酸的免疫调节作用研究

目的研究枇杷叶三萜酸(TAL)的免疫调节作用。方法以环磷酰胺(Cy)诱导小鼠免疫低下模型,采用碳廓清试验、溶血空斑试验、二硝基氟苯(DNFB)诱导的迟发性超敏反应及T淋巴细胞亚群的测定,观察TAL对免疫低下小鼠非特异性免疫、体液免疫及细胞免疫的影响。结果TAL(75,225,675 mg.kg-1)ig给药能改善Cy诱导的免疫低下小鼠碳廓清指数(K值)和吞噬指数(α值)的降低;提高Cy诱导的免疫低下小鼠血清IgM和IgG的生成,增加Cy诱导的免疫低下小鼠脾细胞溶血素的产生;增强Cy引起的免疫功能低下小鼠的DTH反应。结论TAL具有良好的免疫调节作用。