副玫瑰苯胺法测定食品中亚硫酸盐的改进

副玫瑰苯胺法测定食品中亚硫酸盐的改进王守娟赵憬一、原理食品中二氧化硫与甲醛缓冲溶液反应后，生成稳定的羟基甲基磺酸与盐酸副玫瑰苯胺作用生成紫红色化合物，与标准系列比较定量。二、仪器与试剂分光光度计。邻苯二甲酸氢钾－甲醛溶液：称取２．０４ｇ邻苯二甲酸氢钾...     

越南入境保鲜龙眼果肉中二氧化硫残留结果分析

目的：检测越南入境保鲜龙眼果肉中二氧化硫残留量，保护国内消费者的身体健康。方法：采用食品中亚硫酸盐的测定（GB／T5009．34—2003）的第一法，即盐酸副玫瑰苯胺法。结果：对7490份样品进行检测，有227份果肉中SO2残留量超过50mg／kg。结论：越南入境的保鲜龙眼SO2残留量超标严重，最高残留为241mg／kg，故要对入境龙眼进行批检测SO2。     

葡萄酒中游离二氧化硫的比色法测定研究

目的引入样品前处理方法,建立一个用甲醛吸收液-盐酸副玫瑰苯胺比色法测定葡萄酒中游离二氧化硫的方法。方法在一定温度和酸性条件下,通入空气,葡萄酒样品中的游离二氧化硫随空气进入吸收管被甲醛吸收液吸收后,生成稳定的羧甲基磺酸,在加入氢氧化钠后释放出二氧化硫,与盐酸副玫瑰苯胺反应生成红色化合物,比色定量。结果用本文的样品前处理方法对葡萄酒样品进行处理后,溶液中存在的颜色、还原性物质等均被除去,对测定结果无干扰。同时由于样品被浓缩,用本比色法测定游离二氧化硫含量还可以降低检出限。本法回收率为92. 5%～96. 8%,相对标准偏差为0. 5%～1. 81%。结论本文建立的样品前处理方法和比色法测定葡萄酒中游离二氧化硫含量,其测定结果准确度高、灵敏度高、精密度好、可靠且实用性强。     

干辣椒及辣椒制品中测定亚硫酸盐含量的比较

目的对盐酸副玫瑰苯胺法测定干辣椒及辣椒制品中测定亚硫酸盐含量的比较。方法国标GB/T5009.34-2003的盐酸副玫瑰苯胺法。结果使用盐酸副玫瑰苯胺法测定辣椒制品中亚硫酸盐含量比干辣椒中亚硫酸盐含量高2～3倍。结论国标中盐酸副玫瑰苯胺法测定干辣椒及辣椒制品中的亚硫酸盐含量灵敏度高,结果准确,可靠。     

非水溶性食品中亚硫酸盐快速测定的研究

目的：建立一种亚硫酸盐快速检测方法。方法：将试样用4．0ml 1．0mol／LNaOH碱解30min。加4．0ml 0．5mol／LH2SO4盐酸副玫瑰苯胺法测定。结果：测定同批产品比原法减少3．5h，结果与原法无显著性差异（P〉0．05），精密度试验标准差0．002，相对标准偏差2．02％，平均回收率99．3％。结论：本法适用于非水溶性食品特别是蜜饯中亚硫酸盐的测定，可以代替国家标准检验方法，值得推广应用。     

三乙醇胺吸收副玫瑰苯胺光度法测定食品中亚硫酸盐

食品中亚硫酸盐的测定方法常用的是四氯汞钠吸收、盐酸副玫瑰苯胺光度法,但四氯汞钠有一定的毒性。我们采用三乙醇胺,可以络合铁、锰等共存干扰离子,对二氧化硫有很好的吸收,并能获得满意的结果。现介绍如下。原理　二氧化硫被三乙醇胺吸收液吸收后生成亚硫酸盐,再与盐酸副玫瑰苯胺和甲醛作用,生成玫瑰紫色化合物。根据其颜色深浅,进行比色定量。仪器与试剂　（１）７２２型光栅分光光度计。（２）吸收液：称取三乙醇胺５－０ｇ,乙二胺甲乙酸二钠０－１ｇ,溶于蒸馏水中,并稀释至１０００ｍｌ。（３）盐酸副玫瑰苯胺溶液：称取０－…     

快速测定食品中亚硫酸盐的方法探讨

食品中亚硫酸盐的卫生标准检验方法是GB／T5009．34—1996盐酸副玫瑰苯胺法(第一法)和蒸馏法(第二法)，国标第一法中二氧化硫标准使用液，临用前必须用新标定的二氧化硫标准液稀释，操作麻烦，并且在配制四氯汞钠吸收液过程中易引起中毒、过敏。第二法需蒸馏，比较费时。2003年8月用蒸馏水直接浸泡样品，使食品中亚硫酸盐溶于水中，     

2011-2012年武汉市口口区食品中二氧化硫残留量分析

目的对7类食品中二氧化硫残留进行检测,为食品安全监管部门提供执行依据。方法样品采集于武汉市口口区主要的大型超市和农贸市场,按照GB／T5009．34-2003（（食品中亚硫酸盐的测定》第一法盐酸副玫瑰苯胺法执行。结果7类食品检测的二氧化硫含量在0．0～8．94研嚷之间,检出率在在24．7％～97.3％之间,合格率在41．3％～95．5％之间。干菜合格率最低,只有41．3％,其次是竹笋、银耳、腐竹,酱腌菜、粉丝粉条和莲子污染情况好于前四类食品。大型超市和农贸市场食品中二氧化硫残留量合格率有明显的差异,大型超市食品中二氧化硫残留合格率高,农贸市场食品中二氧化硫残留合格率低。结论监测的七类食品中二氧化硫残留的食品安全问题不容乐观,应当引起有关执法部门重视,需要加大监管力度。     

延平区市场食品中残留二氧化硫的调查

目的：对送检的黄花菜、大米及其制品、蜜饯等进行亚硫酸盐检测，以了解食品中二氧化硫含量，并提出建议。方法：对黄花菜、大米及其制品、蜜饯等食品用GB／T5009．34—2003盐酸副玫瑰苯胺法检测。结果：43份样品中符合国家卫生标准12份，超标31份。结论：市区部分食品中亚硫酸盐的含量超标严重，特别是黄花菜。     

盐酸副玫瑰苯胺比色法测定大蒜类及其制品中亚硫酸盐含量刍议

目的 探讨盐酸副玫瑰苯胺法测定大蒜中的亚硫酸盐的检验方法.方法 国标GB/T5009.34-2003的盐酸副玫瑰苯胺法.结果 使用盐酸副玫瑰苯胺法测定大蒜中的亚硫酸盐含量,结果不准确.结论国标中的盐酸副玫瑰苯胺法不适合测定大蒜类食品中亚硫酸盐的含量.     

Calcium intake in the elderly: validation of a dietary questionnaire

Epidemiological studies of calcium and osteoporosis have been hampered by the lack of a suitable tool for assessing calcium intake. This report describes a new frequency and amount questionnaire for measuring present and past calcium intake in the elderly. The validity of the questionnaire was tested against two commonly used standards of dietary assessment, five-day duplicate diets and seven-day weighed dietary inventories. The resulting correlation coefficients were, respectively, r = 0.76 and r = 0.69, while that for repeatability was r = 0.84. Furthermore, the questionnaire categorized subjects into thirds of the distribution of intake with almost no gross misclassification. It is suggested that the present findings may be extended to the majority of normal, healthy elderly subjects, implying wide application for the questionnaire in the assessment of calcium intake in the elderly.     

Self-reported health problems among Swedish miners one year after unemployment

Unemployment is considered to be a public health concern sincedeterioration in the health of the unemployed is often anticipated.However, for some groups, such as miners, unemployment mightimprove health due to a cessation of potentially harmful occupationalexposures. This study evaluates the health of 79 miners in oneSwedish iron-ore mine, and 226 age-matched controls from thegeneral population, during one year after the closure of themine. The participants received a questionnaire regarding medicalhistory and subjective symptoms at the beginning of the studyperiod, and after one year. Statistically significant negativeeffects on self-reported health attributable to unemploymentwere not found, although neuropsychiatric symptoms were morecommon among the unemployed miners. The miners reported a statisticallysignificant improvement in grip force (p=0.031). They had asignificantly higher prevalence of symptoms associated withmining related exposures when compared with the population controls;pain in the upper extremities [relative risk (RR)=2.27, 95%confidence interval (Cl)=1.44–3.59), back pain (RR=1.84;Cl=1.237–2.75), vasospastic disease of the fingers (RR=2.05;Cl=1.18–3.57) and obstructive respiratory symptoms (attacksof dyspnea and wheezing: RR=3.67; Cl=1.167–11.6).     

Excès de cas humains et animaux de tularémie en France au cours de l’hiver 2007–2008 : émergence ou phénomène isolé ?

Context

Tularemia is a zoonosis affecting humans and hares in France. We describe the results of surveillance in both species, in 2007 and 2008.

Methods

Human tularemia cases are mandatorily notifiable in France since 2003. In hares, surveillance relies on volunteer hunter associations in all districts of the country. Data from mandatory reports and volunteer surveillance in 2007/2008 were analyzed and compared with previous results.

Results

In 2007/2008, 144 cases were reported in humans and 117 cases in hares. This was a 100% increase compared to previous years. Human cases differed from those of previous years only by the frequency of contact with breeding animals. Human cases without any documented risk exposure were also more frequent.

Conclusion

An increase of tularemia cases occurred in 2007/2008 in both species. Complementary studies are needed to identify the species reservoir in France to understand the causes of this peak of cases.     

Occupational health hazards in mining: an overview

This review article outlines the physical, chemical, biological, ergonomic and psychosocial occupational health hazards of mining and associated metallurgical processes. Mining remains an important industrial sector in many parts of the world and although substantial progress has been made in the control of occupational health hazards, there remains room for further risk reduction. This applies particularly to traumatic injury hazards, ergonomic hazards and noise. Vigilance is also required to ensure exposures to coal dust and crystalline silica remain effectively controlled.     

Manufacture and use of nanomaterials: current status in the UK and global trends

This paper provides an overview of the production and use of nanomaterials (NMs), particularly in the UK. Currently, relatively few companies in the UK are identifiable as NM manufacturers, the main emphasis being the bulk markets in metals and metal oxides, and some niche markets such as carbon nanotubes and quantum dots. NM manufacturing in the UK does not reflect the global emphasis on fullerenes, nanotubes and fibres. Some assumptions have been made about the types of NM that are likely to be imported into the UK, which currently include fullerenes, modified fullerenes and other carbon-based NMs including nanotubes. Many university departments, spin-offs and private companies have developed processes for the manufacture of NMs but may only be producing small quantities for research and development (R&D) purposes. However, some have the potential to scale up to produce large quantities. The nanotechnology industry in the UK has strong R&D backup from universities and related institutions. This review has covered R&D trends at such institutions, and appropriate information has been added to a searchable database. While several companies are including NMs in their products, only a few (e.g. manufacturers of paints, coatings, cosmetics, catalysts, polymer composites) are using nanoparticles (NPs) in any significant quantities. However, this situation is likely to change rapidly. There is a need to collect more information about exposure to NPs in both manufacturing and user scenarios. As the market grows, and as manufacturers switch from the micro- to the nanoscale, the potential for exposure will increase. More research is required to quantify any risks to workers and consumers.     

深圳公立医院管理体制改革制度设计

深圳公立医院管理体制改革实行政事分开、管办分开,在理事会架构下按法定机构模式组建市医管中心,落实公立医院运营管理自主权.作者从当前公立医院管理体制的弊端入手,介绍了深圳市进行公立医院管理体制改革的基本思路及改革方案设计的主要举措,深入剖析了的改革方案的特点,并对改革效果进行了预测.     

Effects of nitrogen dioxide on red blood cells of rats: Changes in components of red cell membranes during in vivo exposure to NO2

Red cell membranes, prepared from red blood cells of rats exposed to 4, 10, or 20 ppm nitrogen dioxide (NO₂) for 1 to 10 days, were examined for evidence of changes in membrane components. Appreciable changes were not found in contents of phospholipid and cholesterol during exposure to 10 ppm NO₂. By contrast, protein content altered with the time of exposure. Moreover, changes in protein composition were observed by employing sodium dodecyl sulfate — polyacrylamide gel electrophoresis. Twenty-four-hour exposure to NO₂ at the concentration above 10 ppm resulted in a marked increase in the percentage of lysophosphatidylethanolamine (LysoPE) to the total phospholipids. The prolonged exposure to 10 ppm NO₂ gave rise to a further increase in LysoPE, whereas the percentage of phosphatidylethanolamine (PE) showed a gradual decrease. A 1-day exposure to 4.0 ppm NO₂ also caused an increase in sialic acid content and decreases in those of PE and hexose. In addition to contents of these components the percentage of LysoPE increased 5 days after exposure and the elevated values were maintained up to the end of exposure period. These results demonstrate that red blood cells in circulation exhibit different membrane properties in terms of lipid and carbohydrate composition during 10 days of exposure to 4.0 ppm NO₂.     

Investigating clusters in the workplace and beyond

Clusters of disease are common and occur in the workplace and in the general community. They often arouse considerable concern among the population. Investigations have sometimes lead to exciting new knowledge, but in general the investigation of clusters is difficult and often unrewarding, especially for community clusters. In the workplace, investigations are more likely to find associations and even new causes, but still many clusters remain enigmatic. Despite this, there are many reasons for investigating clusters, including allaying community concern and identifying uncontrolled exposures. A structure for investigating clusters in the workplace is suggested.