首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 734 毫秒
1.
目的 观察金黄地鼠实验性肺气肿肺泡Ⅱ型上皮表面活性物质蛋白(SP)A和B的变化。方法 金黄地鼠气管注入弹性蛋白酶后,分别于注入弹性蛋白酶的30、60和90d处死,肺组织经光镜检查和图像分析后,再行SP-A和SP-B免疫组化染色和形态定量;同时进行透射电镜观察。结果 注入弹性蛋白酶后第30、60和90d肺平均内衬间隔明显增大(P〈0.01);免疫组化显示注入弹性蛋白酶后30d肺泡Ⅱ型上皮细胞SP-A  相似文献   

2.
目的:探讨人支气管肺泡灌洗液和体外气液界面无血清培养的人气管上皮细胞分泌液对绿脓杆菌的抗菌作用。方法:采用酸性尿素聚丙酰胺凝胶电泳(AU-PAGE)分析人支气管肺泡灌洗液阳离子蛋白成分:气液界面无血清培养人气管上皮细胞;电泳凝胶琼脂糖弥散法和琼脂糖弥散法检测支气管肺泡灌洗液及培养细胞分泌液的抗菌活性。结果:人支气管肺泡灌洗液和培养气管上皮细胞分泌液对绿脓杆菌有很强的抗菌作用;随着盐浓度增加,抗菌作  相似文献   

3.
合成多肽为半抗原制备抗SCF受体单克隆抗体   总被引:1,自引:2,他引:1  
本文成功地建立了以合成的小分子肽与蛋白质交联的偶联物为免疫原,制备单克隆抗体(mAb)的技术路线。选定SCF受体特异性肽段进行人工合成,将其偶联于BSA免疫动物,应用细胞融合技术制备了抗两肽段的杂交瘤细胞7株。ELISA法和免疫印迹结果显示,其分泌的mAb特异性强,与偶联载体及多种多肽生长因子均无交叉反应。经APAAP法鉴定,抗胞内羧基端肽的mAbSB304与SCF受体表达细胞系HEL有弱的阳性反应;免疫组化测定与胎儿皮肤基底层色素细胞及胃粘膜上皮细胞呈阳性反应,提示mAbSB304可识别天然状态的SCF受体。  相似文献   

4.
涎腺腺样囊性癌的免疫组织化学及免疫电镜观察   总被引:1,自引:0,他引:1  
采用抗平滑肌的肌动蛋白(actin)、肌球蛋白(myosin)、S—100蛋白和胶质纤维酸性蛋白(GFAP)对4例涎腺腺样囊性癌进行免疫组化和免疫电镜研究。结果发现,该癌中肿瘤性肌上皮细胞对抗actin、myosin和S—100蛋白反应阳性,对抗GFAP反应阴性。这些细胞衬里在囊样腔隙周边、小导管外周或散在于上皮团块中;肿瘤性腺上皮细胞对上述抗体反应阴性。讨论了肿瘤性肌上皮细胞的分化趋向和免疫特性。  相似文献   

5.
对BALB/c小白鼠甲状腺滤泡上皮细胞从胚胎14d(E14d)至成年期的发育过程,进行了组织化学与立体计量学研究。结果表明:(1)E14d~15d,细胞排列成不规则的团和索状。E16d~E17d,细胞围成原始的甲状腺滤泡。E18d,滤泡腔内出现PAS反应阳性胶体,成熟的甲状腺滤泡结构基本形成。(2)胚胎期滤泡上皮细胞核较大,核/质比大于1.0,分裂像易见。出生后21d,核/质比稳定在0.35,滤泡上皮细胞形态结构接近成年期状态。(3)E16d,AcP、ATPase、MAO和SDH均呈阳性反应,随后酶活性逐步增强,至生后21d均达强阳性反应。  相似文献   

6.
α-MSH对EGTA性发热效应及脑腹中隔AVP含量的影响   总被引:1,自引:0,他引:1  
目的和方法:观察脑腹中隔精氨酸加压素(AVP)在α-黑素细胞刺激素(α-MSH)解热机制中的作用;用放射免疫法测定AVP含量。结果:EGTA引起明显的发热反应(P<0.01),同时降低脑腹中隔AVP含量(P<0.05)。α-MSH可抑制EGTA性发热反应(P<0.01),并增加脑腹中隔AVP含量(P<0.05);而对正常家兔体温及脑腹中隔AVP含量无影响(P>0.05)。结论:α-MSH对EGTA的解热作用可能部分是通过增加脑腹中隔AVP的释放而实现的。在限制发热的过程中,内生解热物α-MSH与AVP可能具有协同作用。  相似文献   

7.
以酶组织化学、分子杂交及放免法观察了慢性阻塞性肺疾病(COPD)患者和对照者肺组织中一氧化氮合成酶(NOS)的分布、mRNA表达及活性。结果表明:NOS广泛分布于对照组支气管、肺泡管和部分肺泡囊上皮细胞及肺血管内皮;COPD组支气管上皮细胞及肺小动脉内皮NOS活性较对照组低,但在肺间质和肺血管周围出现NOS阳性细胞增多;斑点和Northern印迹杂交显示COPD组肺组织NOS基因mRNA水平较对照组明显低;COPD组肺组织和肺小动脉环磷酸鸟苷含量均较对照组明显降低(P<0.01)。提示一氧化氮在肺脏中发挥重要生理作用及在COPD发病中可能具有重要意义。  相似文献   

8.
α—MSH对家兔ET性发热反应及脑腹中隔区AVP含量的影响   总被引:3,自引:7,他引:3  
目的:研究脑腹中隔区精氨酸加压素(AVP)在α-黑素细胞刺激素(αMSH)解热机制中的作用。方法:建立家兔ET性发热模型,观察侧脑室注射α-MSH对家兔ET性发热反应及脑腹中隔区AVP含量的影响。结果:(1)静脉注射ET(03μg/kg)引起家兔明显的发热反应(P<0001),并增加脑腹中隔AVP含量(P<005);(2)静脉注射ET(03μg/kg)30min后,侧脑室注射α-MSH(200ng/只),能明显抑制家兔发热反应,同时脑腹中隔区AVP含量进一步显著增高(P<0001);(3)侧脑室注射α-MSH(200ng/只)并不影响家兔正常体温,但增加脑腹中隔区AVP含量(P<005)。结论:α-MSH的解热作用可能部分是通过腹中隔AVP增多来实现的,αMSH可能是引起发热时脑腹中隔区AVP含量增加的一个重要因素。  相似文献   

9.
斑点杂交及RNA酶保护分析法检测反义HSP90基因转染细胞 …   总被引:1,自引:1,他引:0  
目的:检测HSP90反义核酸转染细胞后反义RNA的表达,方法:采用打点杂交及RNA酶保护分析法,结果:HSP90反义核酸转染细胞AH-SGC7901,AH-SGC7901/VCR,AH-HCC7402及AH-Ec109有HSP90反义RNA的表达。结论:ESP90反义RNA在AH-SGC7901,AH-SGC7901/VCR,AH-HCC7402及AH-Ec109细胞的表达,为进一步研究HSO90  相似文献   

10.
新近报道牛纤毛柱状上皮细胞表达抗菌肽β-防御素,人气管上皮细胞是否分泌β-防御素尚存疑。本文对气液界面培养的人气管上皮细胞的抗菌活性和人支气管肺泡灌洗液抗菌多肽进行了分析。用低温酶消化法获取健康成人新鲜气管粘膜上皮细胞,采用气液界面无血清培养方式培养,一周后用PBS反复冲洗套皿及底层24孔板,撤去培养基内的抗生素,继续培养,并每隔48h收集套皿内上清及底层培养基。采用琼脂糖弥散法检测其抗菌活性。结果显示培养细胞呈现复层生长,细胞角蛋白免疫组化染色呈阳性反应,可确认为气管上皮细胞。抗菌实验显示培养…  相似文献   

11.
扬子鳄胚胎气管,支气管的发生   总被引:4,自引:0,他引:4  
华田苗  陈壁辉 《解剖学报》1995,26(2):219-221
通过对28例扬子鳄胚胎气管、支气管的发生过程的观察,发现孵化第6d出现喉气管沟,第8d形成气管芽和支气管芽。第14-40d中,气管与支气管上皮周围的间充质细胞逐渐聚集,并分化形成粘膜下层、软骨片及外膜的纤维膜。第20-46d中,气管及支气管的上皮由复层柱状上皮逐步演弯为单层或假复层柱状上皮。本文还对扬子鳄气管、支气管组织发生的部分特点作了讨论。  相似文献   

12.
人胚胎胃的组织发生   总被引:3,自引:0,他引:3  
张薇  何素云 《解剖学报》1992,23(3):325-329
  相似文献   

13.
离体金黄地鼠气管器官培养的实验形态学研究   总被引:1,自引:0,他引:1  
本文报告正常状态下金黄地鼠的体外器官培养变化。应用旋转培养方法,培养液用RPMI 1640及Eagle MEM,不论是否加小牛血清,培养的气管组织均可存活到8周。组织学及超微结构观察表明,培养1~4周内的气管上皮,保持着复层或单层柱状上皮结构,表面尚见纤毛,上皮细胞还没有明显的胞浆变性。细胞器如粗面内质网及线粒体等,还未见破坏。表层上皮细胞间的连接,也是常态的紧密连接。培养5周时,上皮表面大部分纤毛脱落,表层上皮细胞核变扁平、横位。7周以后,细胞结构出现退行性变化。放射自显影观察表明,上皮细胞核内仍有明显的~3HTdR掺入。本文认为,在一般条件下培养4周以内,气管上皮的超微结构较为完整,此时附加实验因素,开展实验病理研究是比较合适的。基础培养液Eagle MEM具有与RPMI 1640培养液相同的培养效果,无论从防止细胞增生引起培养组织的消耗,还是从经济观点考虑,在器官培养中使用基础培养液是值得推荐的。  相似文献   

14.
The development of the tracheal epithelium was studied in hamsters, beginning on fetal day 10 and ending on fetal day 16, shortly after birth. The epithelial morphology was characterized and as soon as the cells could be recognized by type (day 14) their proportions were quantified along dorsal and ventral surfaces from larynx to carina. At all times, columnar cells of the dorsal surface were taller than those of the ventral surface. On days 10 and 11 the simple epithelium was composed of poorly differentiated columnar cells, but on day 12 organoid clusters, consistent with the morphology of neuroepithelial bodies, were observed; four clusters were seen along the dorsal epithelium in one section. The epithelium was pseudostratified on day 13, composed of short and columnar cells. Most columnar cells were poorly differentiated but a few preciliated and ciliated cells were recognizable dorsally, especially at the tracheal poles. Hemidesmosomes were seen at the base of some short cells on day 14, and rough endoplasmic reticulum was moderately developed in some columnar cells, suggesting that these cells were prebasal and presecretory cells, respectively. Preciliated and ciliated cells, which were most prevalent caudally, accounted for about 14% of all dorsal epithelial cells on day 14, but they were rare in the ventral surface, about 0.1%. The epithelial cells were sufficiently specialized by days 15 and 16 to allow quantification by type. Proportions of basal, presecretory, and preciliated-ciliated cells were similar on both days but the cellular makeup of dorsal and ventral surfaces was significantly different. There were more basal cells ventrally (36-40%) than dorsally (22-23%), and more preciliated-ciliated cells dorsally (18-21%) than ventrally (about 1%). On days 15 and 16 differences also existed along both surfaces between cranial and caudal parts of the trachea. Basal cells were more prevalent cranially and preciliated-ciliated cells were more prevalent caudally.  相似文献   

15.
In the larynx of the Suncus murinus the stratified squamous epithelium lines the tip of the caudal surface of the epiglottis and the region close to the vocal process of the arytenoid cartilage, while the ciliated columnar epithelium is located in the laryngeal sac and the dorsal part of the vocal fold. Further, in the transitional zone between the stratified squamous epithelium and the ciliated columnar one there exists the epithelium which shows gradations ranging from stratified squamous through stratified cuboidal to ciliated stratified low-columnar type. It is suggested that the epithelium lining the transitional zone is identical with the "intermediate epithelium" in the mouse nasopharynx and larynx.  相似文献   

16.
The purpose of this work was to study the human fetal esophagus maintained in organ culture. Esophageal explants from 8 fetuses aged from 12 to 16 weeks of gestation were cultured up to 21 days at 37 degrees C in Leibovitz L-15 serum-free medium. Between 12 and 16 weeks of gestation, the esophagus has a stratified columnar ciliated epithelium, and glycogen aggregates are present in all cell layers. This morphology remains the same up to 5 days in culture. After 7 to 9 days, a vacuolization in the upper half layer occurs, leading to a lifting off of the ciliated layer and a flattening of the subjacent cells. After 15 days of culture, the esophageal epithelium is stratified squamous and the cells are exfoliated at the surface of the explants. Glycogen aggregates are still present in all layers. Islets of ciliated cells resting on the basal cell layers develop within the squamous epithelium. With the extension of the culture period up to 21 days, the general morphology of the epithelium does not change. The ultrastructural features of the newly formed squamous epithelium, with its basal lamina, are similar to that reported for human adult esophageal epithelium. During the course of the culture, the DNA synthesis continues as determined by autoradiography. It is concluded that it is possible to maintain viable human fetal esophagus in organ culture and that an accelerated maturation takes place leading to the formation of the adult esophageal epithelium.  相似文献   

17.
氟尿嘧啶引起大鼠气管损伤及修复过程的观察及解析   总被引:13,自引:0,他引:13  
目的 进行气管干细胞的定位研究。方法 使用氟尿嘧啶(5-FU)造成大鼠离体气管环的严重损伤,采用光镜、电镜及免疫组织化学链霉素抗生物素蛋白-过氧化物酶(SP)法检测增殖细胞核抗原(PCNA)动态观察气管黏膜的修复过程。结果 5-FU作用12h后,气管上皮脱落,可见少量间隔分布的类似裸核的细胞呈钉状位于基底膜上。去除5-FU 6h后,由扁平上皮覆盖;电镜下可见这些细胞缺乏分化;可见多个PCNA核染色阳性的细胞与1个阴性细胞(G0期细胞)的比例间隔分布;9h后,电镜下可见向纤毛细胞及黏液细胞分化;48h后,气管环全部由假复层纤毛柱状上皮细胞覆盖。结论 5-FU特异性地使进入细胞周期中的细胞变性坏死,而对G0期的细胞没有作用,残留于基底膜上的裸核样的G0期细胞增殖分化,再生出小黏液颗粒细胞和纤毛细胞进而修复损伤的气管环,证明气管干细胞位于气管上皮基底膜上的G0期细胞中。  相似文献   

18.
Cell proliferation in the developing human esophagus was investigated by autoradiography using organ culture. The sites of [3H]-thymidine uptake were localized in the epithelium, the mesenchyme, and the muscularis externa of fetal esophageal explants from 10 to 16 weeks of gestation. Proliferating cells were abundant throughout the stratified epithelium at 10 weeks of gestation. Many labeled nuclei in the mesenchyme and the muscular layer were observed. With the development of the stratified columnar ciliated epithelium, a confinement of the proliferating zone in the basal cell layers occurred, and ciliated cells never appeared labeled. The quantitation of proliferating cells showed a labeling index at its highest value between 10 and 12 weeks; this drastically decreased between 12 and 14 weeks' gestation. A similar pattern was noted for the mesenchyme, while the labeling index in the muscularis externa peaked during the 11-14 weeks period. In all fetuses, the highest labeling index was always recorded in the epithelium. The biochemical quantitation of the [3H]-thymidine uptake into the total esophageal DNA clearly supported the continuous decrease of the cell proliferation determined by autoradiography between 10 and 16 weeks of gestation. The present investigation provides for the first time basic quantitative data regarding cell proliferation during a particular developmental phase of the human esophagus.  相似文献   

19.
The paper compares ultrastructural regeneration of the tracheal epithelium on patch grafts coated or not coated with collagen. A longitudinal patch window 2 x 1 cm (four cartilaginous rings in cephalocaudal extent) was made on the ventral wall of the cervical trachea in dogs, and replaced by a polypropylene Malex mesh graft. Grafts coated with collagen allowed normal connective tissue ingrowth and subsequent epithelial spreading. From the cut edge, flat, stratified, poorly differentiated cells migrated and spread on the newly formed stroma. Six weeks after the operation, the prosthesis was thoroughly covered by pseudostratified ciliated columnar epithelium with a newly formed basal lamina. On uncoated grafts, in contrast, some regenerated ciliated cells formed compound cilia or irregular microvilli and dilated endoplasmic reticulum. At 6 weeks, no normal basal cells differentiated, although some bizarre flat cells with extremely extended cytoplasm were located in the basal area of the epithelium. The basal lamina was thick and discontinuous. The underlying stroma included abnormally elongated fibroblasts with condensed cytoplasm and curved, randomly dispersed collagen fibrils. These ultrastructural results indicate that (1) regenerated epithelial cells were derived from poorly differentiated cells; (2) a plastic implant may lead to abnormal regeneration in the connective tissues and epithelium; and (3) collagen coating of the graft may allow fibroblasts to produce normal connective tissue substances.  相似文献   

20.
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号