银屑病患者治疗前后血清Ⅳ型胶原和层粘连蛋白测定的意义

Ⅳ型胶原 (ＣｏｌｌａｇｅⅣ ,Ⅳ .Ｃ)是细胞外基质的重要成分 ,在正常情况下分布在血管、胆管的基底膜中。层粘连蛋白(Ｌａｍｉｎｉｍ ,ＬＮ)为细胞外间质中的非胶质糖蛋白 ,广泛分布于基底膜的透明层 ,与Ⅳ型胶原结合构成基底膜的骨架 ,影响细胞粘附、迁移、调节细胞的生长与分化 ,并与纤维化、肿瘤等疾病密切相关[1] 。国内尚少见有银屑病患者治疗前后血清Ⅳ型胶原和层粘连蛋白测定的报道 ,为此 ,我们进行了探讨 ,现将结果报告如下。对象和方法一、对象 :(一 )正常人 :35人 (男 2 5 ,女 10 ) ,均为我院预防保健科体检合格的健康者 ,均无…     

系统性红斑狼疮患者血清IV型胶原和层粘连蛋白测定的意义

为探讨血清IV型胶原 (IV C)和层粘连蛋白 (LN)在系统性红斑狼疮 (SLE)患者中的临床意义 ;我们采用放射免疫法(RIA)对 34例SLE和 6 3例正常人的血清IV C和LN含量进行了测定 ,并进行治疗前后对比以及血清IV C和LN水平与其它临床指标间的直线相关关系分析。结果发现SLE组血清IV C和LN均较对照组显著升高 (P <0 0 0 0 1) ;治疗后随病情缓解较治疗前明显降低 (分别为P <0 0 0 1;0 0 0 5 )。血清LN与血清白蛋白呈显著负相关 (P <0 0 0 1) ,与 2 4h尿蛋白定量、血清免疫球蛋白M和补体C3水平之间呈明显的正相关 (P <0 0 5 ) ;血清IV C与血沉呈显著正相关 (P <0 0 5 )。上述结果提示血清IV C和LN水平在SLE患者中普遍升高 ,是评估SLE患者病情活动的重要指标     

系统性红斑狼疮患者血清Ⅳ型胶原和层粘连蛋白测定的意义

为探讨血清Ⅳ型胶原（Ⅳ．C）和层粘连蛋白（L)居系统性红斑狼疮（SLE）患者中的临床意义;我们采用放射免疫法（RIA）对34例SLE和63例正常人的血清Ⅳ．C和LN含量进行了测定,并进行治疗前后对比以及血清Ⅳ．C矣LN水平与其它临床指标间的直线相关关系分析。结果发现SLE组血清Ⅳ．C和LN均较对照组显著升高（P＜0．0001）;治疗后随病情缓解较治疗前明显降低（分别为P＜0．001;0．005）。血清LN与血清白蛋白呈显著负相关（P＜0．001）,与24h尿蛋白定量、血清免疫球蛋白M和补体C3水平之间呈明显的正相关（P＜0．05）;血清Ⅳ．C与血沉呈显著正相关（P＜0．05）。上述结果提示血清Ⅳ．C和LN水平在SLE患者中普遍升高,是评估SLE患者病情活动的重要指标。     

小鼠附睾Ⅳ型胶原和层粘连蛋白的分布

目的 研究附睾发育过程中Ⅳ型胶原和层粘连蛋白（ｌｉａｍｉｎｉｎ,ＬＮ）的定位和分布。方法 应用生物素－抗生素ＤＣＳ体系间接免疫荧光染色技术。结果 １５ｄ幼鼠附睾基膜内Ⅳ型胶原和ＬＮ荧光强度较弱;３个月成年鼠附睾基膜内Ⅳ型胶原和ＬＮ荧光强度最强;１８个月老年鼠附睾基膜内Ⅳ型胶原和ＬＮ荧光强度比３个月弱。３月龄成年小鼠附睾各部分的荧光强度从头部至尾部呈逐渐增强的趋势。     

宫颈鳞癌基底膜Ⅳ型胶原及层粘连蛋白的免疫组化观察

采用免疫组织化学法染色,标记基底膜的特异性成分Ⅳ型胶原、层粘连蛋白对宫颈鳞状上皮不典型增生、原位癌,早浸癌及浸润癌的基底膜变化进行观察研究。结果显示正常及不典型增生情况下基底膜是完整,连续的。原位癌基底膜通常是连续的,偶有中断。早浸癌基底膜通常是断续的,偶尔癌巢周围可出现完整、连续的基底膜。浸润癌基底膜的表现与细胞分化有关,分化好的癌巢周围存在基底膜,分化差的则缺少基底膜。基底膜变化的机理可能在于合成和分解代谢的不平衡。     

层粘连蛋白受体、Ⅳ型胶原的表达及微血管密度与乳腺癌转移的关系

目的：探讨乳腺癌组织层粘连蛋白受体（LN－R）、Ⅳ型胶原（Col Ⅳ)、间质微血管密度与乳腺癌淋巴结转移的关系。方法：应用免疫组织化学S－P方法标记82例乳腺癌组织中LN－R、Col Ⅳ、FⅧRAg，并在第Ⅷ因子相关抗原（FⅧRAg)标记切片上计算微血管密度（MVD）。结果：LN－R表达强度及MVD的高低在乳腺癌有淋巴结转移组和无淋巴结转移组间有差异；ColⅣ在两组间无差异。结论：乳腺癌间质微血管密度增加及LN－R表达增强可作为预测肿瘤转移及判断预后的指标。     

小鼠卵巢Ⅳ型胶原、间质金属蛋白酶和层粘连蛋白的合成、分布

目的　研究 型胶原、层粘连蛋白 (lam inin,L N)和间质金属蛋白酶 - 2 (matrix m etalloproteinase- 2 ,MMP- 2 )在不同年龄小鼠卵巢中的分布和变化模式。　方法　利用生物素 -抗生物素蛋白间接免疫荧光方法 ,从蛋白水平观察 型胶原和 L N的分布和变化 ;利用地高辛标记的 型胶原和 MMP- 2基因探针进行原位杂交 ,从m RNA水平观察 型胶原和 MMP- 2的分布和变化。　结果　出生 5 d小鼠卵巢的卵泡基膜和卵巢表面上皮(ovarian surface epithelium,OSE)基膜处已有少量 型胶原和 L N分布 ,基质细胞和 OSE中也有分布。 型胶原和 MMP- 2的 m RNA在基质细胞和 OSE有表达。发育期小鼠卵巢中卵泡基膜和 OSE基膜中 型胶原和 L N的含量随年龄的增加而呈增加趋势 ,分布也趋于连续。颗粒细胞、膜细胞、OSE中都有 型胶原和 L N分布 ,也有 型胶原和 MMP- 2 m RNA的表达 ,其表达水平明显比 5 d时高。成熟期小鼠卵巢 型胶原和 L N的含量较高。12个月的小鼠卵巢已开始退化 ,基膜细胞外间质 (extracellular matrix,ECM)的数量减少 ,MMP- 2 m RNA的表达量无明显变化。　结论　 型胶原和 L N在小鼠卵巢发育过程中含量和分布发生着动态变化 ,可能对卵泡发育起重要作用     

Ⅳ型胶原和Laminin在人体慢性肝病和肝癌中的病理学研究

目的：观察基膜中Ⅳ型胶原（ＣｏⅣ）及Ｌａｍｉｎｉｎ（ＬＭ）在人体慢性肝病和肝细胞癌（ＨＣＣ）的病理形态学改变,并探讨其临床病理意义。方法：对１１例慢性肝炎、２６例肝硬化和３０例人体ＨＣＣ组织进行了ＣｏⅣ和ＬＭ的免疫组化检测,系统地观察基膜成分在正常、慢性肝病和肝癌中的形态和数量的改变,行半定量计数,对其结果进行临床病理分析。结果：正常肝窦无ＬＭ的表达,ＣｏⅣ沿窦壁呈断续弱阳性表达,慢性肝病时可有少     

The respiratory system in connective tissue disorders

The connective tissue disorders (also called collagen vascular diseases) represent an heterogeneous group of immunologically mediated inflammatory disorders with a large variety of affected organs besides the lungs. The respiratory system may be involved in all its components: airways, vessels, parenchyma, pleura, respiratory muscles, etc. The frequency, clinical presentation, prognosis and response to therapy vary, depending on the pattern of involvement as well as on the underlying connective tissue disorders. The subject of this review is to describe the most frequent type of lung disorders observed in patients with connective tissue disease (CTD). We will focus on the most frequent CTD: systemic lupus erythematosus, rheumatoid arthritis, scleroderma, Sjogren's syndrome, dermatopolymyositis and mixed CTD.     

Anti-DNA antibodies cross-reacting with laminin inhibit trophoblast attachment and migration: implications for recurrent pregnancy loss in SLE patients

PROBLEM: Systemic lupus erythematosus (SLE), an autoimmune disease, is associated with reduced fetal survival, recurrent abortions, and other pregnancy complications. Some of the autoantibodies found in SLE bind to laminins (LNs), which play an important role in the implantation of the fertilized ovum in humans. METHOD OF STUDY: To elucidate the role of these specific autoantibodies, chorionic villous explants from 6 7-week-old human placentas were established as organ cultures on laminin-1 (LN-1), collagen IV (CN-IV) or uncoated culture dishes. The cultures were then exposed to a mouse monoclonal anti-DNA/anti-LN-1 antibody, to human polyclonal lupus antibodies cross-reacting with LN-1, a function-blocking polyclonal antibody to LN-1, polyclonal antibodies to CN-IV, or IgG control. RESULTS: The explants attached to LN-1 and CN-IV, but not to uncoated culture dishes. LN-1 promoted migration of trophoblast, whereas CN-IV promoted migration of fibroblast-like cells. Trophoblast attachment and migration were abolished in a dose-dependent manner by all three antibodies to LN-1, but not by antibodies to CN-IV or IgG control. Furthermore, the effect of anti-LN antibodies was abolished by preincubating them with LN-1. CONCLUSIONS: These studies suggest that anti-DNA antibodies cross-reacting with LNs may play a role in early pregnancy failure in SLE patients by interfering with placental implantation.     

Increased serum levels of soluble interleukin-2 receptor in patients with systemic lupus erythematosus and rheumatoid arthritis

In this study we investigated the serum levels of a released soluble form of the interleukin-2 receptor (sIL-2R) in 42 patients with rheumatoid arthritis and in 12 cases of systemic lupus erythematosus. Data were evaluated in relationship to the clinical phase and compared with those observed in normal controls (N=56) and in osteoarthritis (N = 7). Increased levels were observed in both rheumatoid arthritis (mean ± SE, 604±49 U/ml) and systemic lupus erythematosus (1438±481 U/ml). These values were significantly higher than in control (256±15 U/ml;P<0.001) and in osteoarthritis (298±33 U/ml;P<0.001) groups. In addition, the highest values were associated with the active phases of both rheumatoid arthritis (active vs inactive, 771±78 vs 451±39 U/ml;P<0.001) and systemic lupus erythematosus (active vs inactive, 2108±489 vs 499±75 U/ml;P<0.001). Our findings suggest that the detection of sIL-2R in rheumatoid arthritis and in systemic lupus erythematosus may represent a good marker of disease activity, which indirectly indicates the ongoing activation and/or proliferation of immunoreactive cells which are involved in the pathogenetic events of these autoimmune conditions.     

Distribution of basement membrane laminin and type IV collagen in human reactive lymph nodes

The location of two basement membrane components, laminin and the 7-S domain of type IV collagen, was studied in human lymph nodes using the peroxidase-antiperoxidase method. Basement membrane antigens were present on the walls of blood vessels and of marginal, trabecular and medullary sinuses. Thin, fragmented fibre-like staining was present also in parenchyma outside the germinal centres, in a pattern overlapping with reticular fibres as seen on conventional reticulin stains. This finding suggests that basement membrane components are a part of the reticular fibres of lymph nodes, or are closely associated with them.     

半乳凝素-1与自身免疫疾病

 自身免疫性疾病是机体对自身抗原发生免疫反应而导致自身组织损害的一系列疾病,其发病机制复杂,T辅助细胞亚群失衡在其中发挥重要作用。半乳凝素-1是一种内源性糖结合蛋白,在T细胞的凋亡、信号传导及细胞因子的分泌等发挥重要的调节作用,有大量研究表明gal-1参与了自身免疫性疾病的发病机制,给予重组gal-1干预可以改善疾病的严重程度,延缓疾病进展,提示其有望成为一个新的自身免疫性疾病的治疗靶点。     

Autoreactive B‐lymphocytes in SLE and RA patients: Isolation and characterisation using extractable nuclear and citrullinated antigens bound to immunobeads

Systemic lupus erythematosus and rheumatoid arthritis are autoimmune diseases characterised by B‐cell hyperactivation and production of autoantibodies (AutoAbs) against various self‐antigens, including extractable nuclear antigens and citrullinated peptides. Therefore, B lymphocytes and antibody‐secreting cells are considered relevant targets for therapies. However, isolation and characterisation of auto‐reactive specific B lymphocytes are limited, primarily due to technical issues. In this work, we purified extractable nuclear antigen‐specific and citrullinated peptide‐specific auto‐reactive B lymphocytes by magnetic selection with ENA‐ and citrullinated peptide‐bound immunobeads. We obtained blood auto‐reactive B lymphocytes from most patients. Their nature was primarily naïve B cells, some of them in an active status, with low levels of somatic hypermutations in the immunoglobulin heavy‐chain variable regions. Their presence correlated with serum levels of autoAb. Auto‐reactive B lymphocytes were able to differentiate into auto‐reactive antibody‐secreting cells under conditions of stimulation. In addition, based on the presence of circulating auto‐reactive B cells and/or antibody‐secreting cells, four different profiles were described in lupus patients. Thus, tracking auto‐reactive B cells and/or antibody‐secreting cells in patient blood could represent a biomarker for deciding whether to use therapies blocking either B cells, plasma cells or both, as well as a new tool for monitoring minimal residual autoimmune disease in patients.     

Immunoreactivity for laminin and type IV collagen in malignant and benign fibrous histiocytoma

Sixteen malignant fibrous histiocytomas (MFH) and ten benign fibrous histiocytomas of the skin were studied immunohistochemically for the distribution of two basement membrane (BM) proteins, laminin and type IV collagen, in order to evaluate their cellular nature. Linear staining for both proteins was present in the vascular BMs. Intracytoplasmic laminin was observed in the neoplastic fibroblast-like and pleomorphic giant cells in 11 MFHs. Two MFHs also showed similar staining for type IV collagen. In the giant cell subtype of MFH, the reactive giant cells were totally negative whereas the neoplastic cells were strongly positive for laminin. Extracellular fibres staining positively for both BM proteins were seen in two MFHs. Except for the capillary network, the benign fibrous histiocytomas were negative for laminin and type IV collagen. On the basis of the present results, we favour the concept that MFHs are primitive mesenchymal tumours, some of which may show histogenetic relationships with tumours of BM forming mesenchymal cells.     

Reticulin and its related structural connective tissue proteins in the rheumatoid synoviume

Argyrophilic reticulin fibres are an important component of the rheumatoid synovium and their distribution and that of their individual protein constituents have been studied in synovial biopsies from a series of 29 cases of rheumatoid arthritis. In acutely inflamed synovia they are predominantly found underneath the hyperplastic synovial lining layer and related to the inflammatory cell infiltrate. With developing chronicity the reticulin network is gradually replaced by mature collagen. This histological pattern is mirrored by changes in the individual components of reticulin fibres-fibronectin, the non-collagenous reticulin component of Pras and Glynn (NCRC) and collagen type III.