Plasma pyridoxal phosphate concentrations of men fed different levels of vitamin B-6

The vitamin B-6 status of 12 white, 3 black, and 7 men of other races was evaluated utilizing plasma pyridoxal phosphate concentrations after the males had been on controlled diets for 8 wk. The diets contained averages of 1.22-1.67 mg/d vitamin B-6 as calculated from food composition tables. The actual mean vitamin B-6 content of these diets as analyzed with Saccharomyces uvarum was 0.75-0.98 mg/d; the mean daily protein intakes were 80.8-84.5 g. All subjects had plasma pyridoxal phosphate concentrations (67.6-125.8 nmol/L) well within the normal range; the values of the different race and dietary groups were similar. Vitamin B-6 requirements were satisfied by the consumption of diets analyzed to contain means of 0.75-0.98 mg/d of the vitamin as demonstrated by plasma pyridoxal phosphate levels indicative of adequate status. Dietary vitamin B-6 intakes should be determined by actual analyses when requirements are being determined.     

Dietary and lifestyle factors affecting plasma vitamin D levels in Asian children living in England.

OBJECTIVE: To determine the vitamin D status of a sample of young Asian children living in England by measurement of plasma 25-hydroxycholecalciferol levels; to relate biochemical measurements with parameters of diet, lifestyle and iron status in this population. DESIGN: The 'Infant Feeding in Asian Families' survey covered infants born to mothers of Bangladeshi, Indian or Pakistani origin, plus a sample of children born to White mothers in 41 local authority areas. The current study describes vitamin D values in a sub sample of Asian children from that survey. SETTING: Forty-one local authority areas which covered 95% of the Asian population at the time of the 1991 Census and include areas with the highest and lowest density of Asians. SUBJECTS: Seventy-one percent (2382) of mothers who were originally eligible were interviewed on four occasions up to the time their infant was 15 months. One thousand, seven hundred and thirty-eight of these children were followed up for this current study. Of these 618 had a blood sample analysed for vitamin D. OUTCOME MEASURES: Previous day's food intake, normal dietary intake, indicators of iron status and plasma vitamin D levels. RESULTS: Plasma vitamin D values are lower in the three groups of Asian children than values reported for children of a similar age in the National Diet and Nutrition Survey. Between one third and a half of children with a low haemoglobin also had low plasma vitamin D values; there was a significant association between failure to take a vitamin supplement, chapati consumption and low vitamin D values. CONCLUSIONS: It is particularly important that pre-school Asian children receive a vitamin D supplement. Children with low haemoglobin values should be screened for vitamin D deficiency.     

Plasma vitamin B12, folate and homocysteine levels in gastrectomized men

BACKGROUND & AIMS: Hyperhomocysteinemia is a risk factor for cardiovascular disease, dementia and depression. Prevalence rate of hyperhomocysteinemia in gastrectomized patients is not well elucidated. METHODS: We compared plasma total homocysteine, vitamin B12 and folate of gastrectomized male patients with those of control subjects. RESULTS: Total homocysteine was higher in gastrectomized patients than in control subjects (11.7 +/- 4.4 micromol/l vs. 9.3 +/- 2.4 micromol/l, P = .011), while vitamin B12 was lower in patients than in control subjects (382 +/- 211 pg/ml vs. 500 +/- 179 pg/ml, P = .020). Folate of the two groups was comparable (5.8 +/- 2.5 ng/ml vs. 5.9 +/- 2.2 ng/ml). Of 31 gastrectomized patients six (19.4%) showed low vitamin B12 (<233 pg/ml), four (12.9%) low folate (<3.0 ng/ml) and seven (22.6%) hyperhomocysteinemia (>14 micromol/l), whereas they were found in one (3.2%), none (0%) and one (3.2%) of 31 control subjects, respectively. Patients who had undergone gastrectomy because of cancer showed higher total homocysteine compared to patients who had undergone it because of peptic ulcer. Type of surgery did not correlate with total homocysteine in the present study. CONCLUSION: Hyperhomocysteinemia is not rare in male Japanese gastrectomized patients.     

Attitudes and risk perception of parents of different ethnic backgrounds regarding meningococcal C vaccination

The aim of the present study is to assess the attitudes of parents toward vaccination as well as their risk perception of disease and vaccination. We interviewed 1763 parents of different ethnic groups (among others, Dutch, Turkish, Moroccan, and Surinamese parents). Results show that there were large differences in knowledge about disease and risk perception of disease and vaccination among parents of different ethnic backgrounds. Generally, people largely overestimated the risk of contracting the disease and the risk of dying after contracting the disease. Dutch parents were best informed, least worried, had the most critical attitude toward the campaign, and the lowest vaccination level compared to other parents. The differences in knowledge about vaccination and the more critical attitude of Dutch parents emphasize the need to take more into account parents' perspectives when designing information leaflets or other information media.     

Plasma vitamin A, E, and beta-carotene levels in adult post-partum Algerian women.

Vitamins A and E are essential for foetal growth, reproduction, and lactation. In this article we report the results of a study, lead in three Eastern Algeria cities, that involved 786 post-partum women and 250 control. Plasma levels of vitamins A, E, beta-carotene, and some nutritional indexes were measured in both groups. In control women, plasma retinol and beta-carotene levels were significantly lower in Algeria than in France (retinol: 1.4 +/- 0.42 vs. 1.78 +/- 0.53 mumol/l; beta-carotene: 0.35 +/- 0.261 vs. 0.94 +/- 0.611). These differences could be the consequence of different beta-carotene and retinol intakes. In Algeria, comparisons between post-partum women and controls, showed that plasma vitamin A and beta-carotene levels were significantly lower in post-partum than in control women. This fact, and the lower level of retinol in control women, raises the question of supplementation for pregnant women in Algeria, at least for those with the lowest standard of living whose protein and zinc levels are also very low after delivery. Plasma vitamin E levels and vitamin E/total lipid ratios were not different in Algeria and in France. Vitamin E concentration was higher during pregnancy, but the vitamin E/total lipid ratio was significantly lower, which shows a relative deficiency at the end of pregnancy. Comparisons of plasma vitamin E levels, at delivery, in primiparous and in multiparous women reveal a better tocopherol status in multiparous women. This difference could reflect an adaptive response to oxidative stress in multiparous women.     

Factors associated with age of smoking initiation in adult populations from different ethnic backgrounds

BACKGROUND: The age at which the individual begins smoking may influence the health of smokers at an older age. Cultural and social factors effect the age of smoking initiation. METHODS: A cross-sectional national survey of a random sample of 6,021 Israeli residents over the age of 21 were interviewed by telephone. The sample consisted of 4248 Jews, 858 Arabs and 915 immigrants, 856 of them were from the former Soviet Union. RESULTS: Compared to the Jewish population the Jews who immigrated from the former Soviet Union began smoking at an earlier age and the Arabs started smoking at an older age. The young respondents in all ethnic groups reported starting to smoke at a younger age compared to the older respondents, and there was a decrease in smoking initiation at an older age. Father smoking during childhood predicted earlier age of smoking initiation but not mother or sibling smoking, significantly among Jews and immigrants and non-significantly among Arabs. CONCLUSIONS: Age of smoking initiation is dependent on the ethnic background in which the smokers grow up, however, the influence of the father smoking seems to be similar in all population groups. It seems that a higher percentage of young adults started smoking at an early age and there is a decrease in smoking initiation at older ages in all ethnic groups.     

Understanding childhood asthma in focus groups: perspectives from mothers of different ethnic backgrounds

Background  

Diagnosing childhood asthma is dependent upon parental symptom reporting but there are problems in the use of words and terms. The purpose of this study was to describe and compare understandings of childhood 'asthma' by mothers from three different ethnic backgrounds who have no personal experience of diagnosing asthma. A better understanding of parents' perceptions of an illness by clinicians should improve communication and management of the illness.     

Plasma testosterone levels in homosexual men

Plasma testosterone levels were measured in 28 homosexual men and correlated with psychological variables. The overall mean testosterone level for the homosexuals was somewhat lower than for a group of heterosexual controls, but the range of values for the two groups showed considerable overlap. Among the homosexuals, the 13 with some heterosexual experience had higher testosterone levels than the 15 with none. Testosterone level was not related to relative masculinity or femininity or to any other psychological variable measured.     

Plasma vitamin C levels are low in premature infants fed human milk

To evaluate the vitamin C nutritional status of premature infants, plasma vitamin C concentrations were measured in 7 neonates born before 32 wk of gestation and in 13 premature infants born at or after 32 wk of gestation. Samples of umbilical venous plasma from 14 full-term infants were analyzed to provide reference values. Oral feedings with pooled, pasteurized milk from human donors were initiated 1-3 days after birth and intake was gradually increased to 200 ml X kg X day during the second week. After 2 wk, the 13 larger infants received approximately half of their daily milk intake from their own mothers. Results showed that plasma concentrations of vitamin C decline rapidly after birth and approach very low levels in preterm infants fed pasteurized, pooled human milk.     

Effects of different levels of vitamin C intake on the vitamin C concentration in human milk and the vitamin C intakes of breast-fed infants

The influence of maternal intake of vitamin C on the vitamin C concentration in human milk and on the vitamin C intakes of breast-fed infants has not been demonstrated conclusively. This study examined these influences of diet and supplementation in 25 lactating women administered 90 mg of ascorbic acid for 1 day followed by 250, 500 or 1000 mg/day for 2 days or unsupplemented for 1 day followed by either 0 or 90 mg ascorbic acid supplement for 2 days. Vitamin C content in milk and urine was determined by the 2,4-dinitrophenylhydrazine method. Vitamin C intakes of infants were calculated from milk volume, as determined by the test-weighing method and from vitamin C levels in milk samples obtained at each feeding. Total maternal intakes of vitamin C, which exceeded 1000 mg/day or 10-fold the RDA for lactation (100 mg/day), did not significantly influence the vitamin C content in milk or the vitamin C intakes of infants. However, maternal vitamin C intake was positively correlated (r = 0.7) with maternal urinary excretion. These differences in milk and urine response to vitamin C intake suggest a regulatory mechanism for vitamin C levels in milk.     

Disturbingly low levels of contraception among women living with hepatitis C

OBJECTIVE: To describe the prevalence of contraception among a sample of women with hepatitis C (HCV), compare it with contraceptive use among Australian women generally, and look for associations between contraception and sample characteristics. METHOD: Women who self-identified as living with HCV were recruited through a wide range of non-clinical and clinical sites in the Australian Capital Territory (ACT) and Victoria to complete a self-administered questionnaire. RESULTS: Seventy-five per cent of distributed questionnaires were completed and returned. Of the 462 women surveyed, 34% of those aged 18-49 reported using contraceptives; a much lower prevalence than the 67% in the Australian population. Surprisingly, women who reported concerns about transmission to children were no more likely to use contraceptives. Not surprisingly, women who were lesbian or who did not have a current partner were even less likely to use contraceptives. Both employed women and those not on benefits reported significantly higher levels of contraception. Otherwise, contraception did not vary with a range of variables including age, education, injecting drug use status, self-rated health status, experience of HCV symptoms, time since diagnosis, ever having received HCV treatment, or venue at which the participants were recruited. CONCLUSIONS: The low prevalence of contraception among women with HCV is both disturbing and puzzling. IMPLICATIONS: These findings raise several important and hitherto unconsidered issues for the sexual and reproductive health and well-being of women with HCV. These require both further research and urgent attention by service providers.     

Blood vitamin and lipid levels in overweight and obese women.

The study was carried out on 102 obese and overweight women, average age 41.5 years, and 33 control non-obese women matched for age. Fasting venous blood vitamins (ascorbic acid, tocopherol, retinol, carotenes, thiamin, riboflavin, folic acid, pyridoxine), lipids (total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, triglycerides, total lipids) and haematological indices were determined. Significantly lower serum antioxidant vitamin levels and a higher prevalence of vitamin deficiency were found in the study group. The study confirmed the high risk of dyslipoproteinaemia and the high frequency of elevated blood pressure in obese women. A correlation between ascorbic acid status, degree of obesity and the incidence of elevated blood pressure was demonstrated.     

Plasma carotenoid and vitamin E concentrations in women living in a rural west African (Gambian) community

Vitamin E and carotenoid pigments are important nutrients for the maintenance of health and protection of tissues against free radical damage. They also play a potential role in disease-risk-prediction and -protection, but little is known about their physiological and sociodemographic correlates and determinants, especially in a West African context. As part of a study of reproductive health in rural Gambian women, blood samples were obtained from 1286 women aged 15 to 54 years, living in the Farafenni area of The Gambia. Measurement of two forms of vitamin E and six carotenoids in plasma was performed by high performance liquid chromatography. All eight components, but especially the carotenoids: lycopene, alpha- and beta-carotene, exhibited a major seasonal variation, with maximum levels between May and July, corresponding to the end of the "mango" season and the beginning of the "rainy" season. Only the tocopherols varied significantly (increasing) with age. Several unexpected ethnic group differences were observed, and canthaxanthin was present at lower concentrations in women with manual occupations, compared to those with non-manual occupations. There were also significant differences associated with pregnancy and the postpartum period, especially for the tocopherols, but fewer differences associated with marital status. The observed patterns confirm that there are important seasonal, physiological, possibly genetic, and sociodemographic determinants of these nutrients in blood plasma, which may have significance for health and longevity.     

Plasma vitamin D biomarkers and leukocyte telomere length in men

Purpose

Vitamin D may reduce telomere shortening through anti-inflammatory and anti-cell proliferation mechanisms. In women, higher plasma 25-hydroxyvitamin D (25(OH)D) has been shown to be associated with longer telomere length, but the relationship has not been assessed in men.

Methods

We conducted a cross-sectional analysis of 25(OH)D, 1,25-dihydroxyvitamin D (1,25(OH)₂D) and relative leukocyte telomere length (LTL) among 2483 men [1832 men for 1,25(OH)₂D] who were selected as cases and controls in three studies of telomeres and cancer nested within the Health Professionals Follow-up Study. We also genotyped 95 SNPs representing common genetic variation in vitamin D pathway genes. LTL was measured by quantitative PCR, and z-scores within each study were calculated. Associations were assessed by linear as well as logistic regression adjusting for age and other potential confounders.

Results

Age (P-trend < 0.0001), pack-years of smoking (P-trend = 0.04) and body mass index (P-trend = 0.05) were inversely associated with LTL. Neither 25(OH)D nor 1,25(OH)₂D was associated with LTL (multivariable-adjusted P-trend 0.69 and 0.41, respectively, for the linear regression model). One SNP in the retinoid X receptor alpha gene was associated with long LTL (P = 0.0003).

Conclusions

In this cross-sectional study of men, 25(OH)D and 1,25(OH)₂D were not associated with relative LTL.

     

Urinary perchlorate and thyroid hormone levels in adolescent and adult men and women living in the United States

BACKGROUND: Perchlorate is commonly found in the environment and known to inhibit thyroid function at high doses. Assessing the potential effect of low-level exposure to perchlorate on thyroid function is an area of ongoing research. OBJECTIVES: We evaluated the potential relationship between urinary levels of perchlorate and serum levels of thyroid stimulating hormone (TSH) and total thyroxine (T4) in 2,299 men and women, > or = 12 years of age, participating in the National Health and Nutrition Examination Survey (NHANES) during 2001-2002. METHODS: We used multiple regression models of T4 and TSH that included perchlorate and covariates known to be or likely to be associated with T4 or TSH levels: age, race/ethnicity, body mass index, estrogen use, menopausal status, pregnancy status, premenarche status, serum C-reactive protein, serum albumin, serum cotinine, hours of fasting, urinary thiocyanate, urinary nitrate, and selected medication groups. RESULTS: Perchlorate was not a significant predictor of T4 or TSH levels in men. For women overall, perchlorate was a significant predictor of both T4 and TSH. For women with urinary iodine < 100 microg/L, perchlorate was a significant negative predictor of T4 (p < 0.0001) and a positive predictor of TSH (p = 0.001). For women with urinary iodine > or = 100 microg/L, perchlorate was a significant positive predictor of TSH (p = 0.025) but not T4 (p = 0.550). CONCLUSIONS: These associations of perchlorate with T4 and TSH are coherent in direction and independent of other variables known to affect thyroid function, but are present at perchlorate exposure levels that were unanticipated based on previous studies.