共查询到18条相似文献,搜索用时 62 毫秒
1.
目的 探索由人羊膜上皮细胞(hAEC)、成纤维细胞与去表皮的真皮(DED)构建组织工程皮肤修复裸鼠全层皮肤缺损的可行性.方法 采用低浓度胰蛋白酶多步消化分离法提取hAEC,并用两步酶消化法处理健康小儿包皮,获得成纤维细胞悬液,传代培养.将体外扩增培养至第3~5代的成纤维细胞和第2代hAEC分别接种在DED真皮面和基底膜面,体外器官培养构建组织工程皮肤.取3~4周龄健康雄性裸鼠20只,抽签法随机分为2组,在所有小鼠背部正中制备全层皮肤缺损模型.组织工程组用构建的组织工程全层皮肤覆盖创面;对照组创面仅覆盖凡士林油纱.分别于术后第7、14、21、28天对裸鼠全身及移植部位大体观察,比较两组间创面愈合时间及创面愈合率,并对移植部位行组织学观察.结果 hAEC具有干细胞特征,免疫荧光显示其表达结合转录因子4(0CT-4)、阶段特异性胚胎抗原4(SSEA-4).器官培养2周后,体外构建的组织工程皮肤形成4~9层复层表皮,且表皮的组织结构形态与正常皮肤类似.移植组织工程皮肤至裸鼠创面后肉眼观察显示,在移植后第7、14、21天,组织工程组创面愈合率分别为57.49%±6.11%、92.80%±3.10%、98.83%±0.25%,均明显高于对照组(22.93%±4.26%、54.57%±7.94%、91.16%±4.79%),差异均有统计学意义(n=10,t值分别为27.36、32.23、11.80,均P<0.001),组织工程组创面愈合时间[(21.51±1.51)d]明显短于对照组[(28.80±1.14)d](n=10,£=42.23,P<0.001),且在移植后第28天移植物颜色与自体皮肤颜色接近.组织学观察显示,组织工程组上皮层次清晰,角化明显,真皮层细胞生长良好;对照组移植区上皮较薄且有部分缺损,真皮层次欠佳,且可见炎症细胞浸润.结论 用hAEC、成纤维细胞复合DED构建的组织工程皮肤移植后能在裸鼠体内存活,且创面愈合更佳,可望成为一种较理想的组织工程皮肤. 相似文献
2.
真皮干细胞(dermalstemcells,DSC)是近年在真皮中分离出的一类成体干细胞,在一定条件下可分化为成纤维细胞、骨细胞、脂肪细胞等。其研究主要集中在皮肤组织工程、创伤修复等方面,但有关其在皮肤衰老中的作用还鲜见报道。皮肤衰老主要与真皮成纤维细胞减少、衰老及其生物学特性改变有关,而DSC在一定条件下通过表达细胞因子能激活或分化为成纤维细胞,进而刺激胶原、弹力蛋白等分泌和合成,增强细胞外基质,促进消除皱纹和增加皮肤弹性,为抗皮肤衰老提供新的途径。 相似文献
3.
表皮干细胞具有无限增殖及多向分化的特性,其子代细胞可以形成角质形成细胞、毛囊、皮脂腺的分化细胞。肿瘤一般起源于单个发生癌性突变的细胞,表皮干细胞很可能是各种类型表皮肿瘤发生的主要靶细胞,分化细胞则能通过影响肿瘤干细胞的克隆扩增,对肿瘤的发生起重要作用。 相似文献
4.
5.
6.
组织工程皮肤是目前组织工程领域研究的热点,也是该领域发展最成熟的。国外许多产品已经被美国食品药品管理局批准进入临床应用。供研究和应用的组织工程皮肤主要包括表皮替代物、真皮替代物、全层皮肤替代物。已广泛应用于烧伤、溃疡、皮肤病的治疗中。 相似文献
7.
人类致命性的疾病多源自器官功能的丧失,而最理想的治疗手段莫过于由人体自身重新长出一个新的组织或器官,对干细胞的探索使人们始终保持着这种“重生”的希望。皮肤作为人体组织的外在屏障,在抵御外环境物理创伤中首当其冲,所以表皮干细胞一直是近年来研究的热点。本文就其研究进展进行了综述。 相似文献
8.
李泓馨 《中国麻风皮肤病杂志》2012,28(1)
表皮干细胞是皮肤重要的干细胞之一,主要位于毛囊隆突部,表面具有多种标记物.表皮干细胞生长于特定微环境niche中,有多种细胞因子、信号通路参与了niche调节表皮干细胞归巢、自我更新和分化,参与皮肤创伤修复、皮肤肿瘤形成等多种功能.对表皮干细胞及其微环境niche的深入研究,对了解表皮发育、表皮肿瘤形成及组织工程有着重要的意义. 相似文献
9.
10.
目的 检测正常人表皮及真皮内结合珠蛋白mRNA的表达。方法 采用逆转录聚合酶链反应 (RT PCR)法。结果 在 12例正常人表皮及真皮总RNART PCR产物中均扩增出结合珠蛋白片段。结论 在正常人表皮及真皮内均有结合珠蛋白mRNA的表达。 相似文献
11.
The depths of hair follicle compartments, and in particular of the bulge, the putative site of hair follicle stem cells, have not yet been determined in human scalp skin from infants, children or adolescents. This information is necessary in order to use the scalp safely as a donor site for skin grafts. We therefore investigated the development of the infundibulum, the bulge, Adamson's fringe, the B-fringe and the matrix by measuring the depths of these five follicular compartments in parietal scalp biopsy specimens from 100 patients ranging in age from 2 weeks to 21 years. The thickness of the epidermis and the dermis were also assessed. The correlations of these measurements with age were determined by regression analysis. The regression equation for the bulge was found to beb (m) =683.3+30.8y (r=0.73; SEM=145.5) wherey is the age in years, and for the matrix it wasm (m) =1616.2+ 90.4y (r=0.76; SEM=406.5);P<0.0001 for the null hypothesis. The growth of the inferior portion below the bulge was not parallel but proportional to that of the superior portion. The relative position of the bulge in the dermis was stable, whereas the inferior portion moved progressively more deeply into the subcutis. These findings provide evidence for the postulated biologically advantageous localization of the bulge, and thus is a further argument in favour of the bulge as the site of follicular stem cells. 相似文献
12.
Desmond J. Tobin 《Journal of tissue viability》2017,26(1):37-46
Cutaneous science has seen considerable development in the last 25 years, in part due to the Omics revolution, and the appreciation that this organ is hardwired into the body's key neuro-immuno-endocrine axes. Moreover, there is greater appreciation of how stratification of skin disorders will permit more targeted and more effective treatments. Against this has been how the remarkable extension in the average human life-span, though in the West at least, this parallels worrying increases in lifestyle-associated conditions like diabetes, skin cancer etc. These demographic trends bring greater urgency to finding clinical solutions for numerous age-related deficits in skin function caused by extrinsic and intrinsic factors. Mechanisms for aging skin include the actions of reactive oxygen species (ROS), mtDNA mutations, and telomere shortening, as well as hormonal changes.We have also significantly improved our understanding of how to harness the skin's considerable regenerative capacity e.g., via its remarkable investment of stem cell subpopulations. In this way we hope to develop new strategies to selectively target the skin's capacity to undergo optimal wound repair and regeneration. Here, the unsung hero of the skin regenerative power may be the humble hair follicle, replete with its compliment of epithelial, mesenchymal, neural and other stem cells. This review introduces the topic of human skin aging, with a focus on how maintenance of function in this complex multi-cell type organ is key for retaining quality of life into old age. 相似文献
13.
目的 探讨体外构建含黑素的组织工程皮肤.方法 采用两步酶消化法处理健康小儿包皮,获得表皮细胞悬液,分别用人角质形成细胞(KC)无血清培养基(SFM)及改良的M254黑素细胞培养基培养KC、黑素细胞(MC),并传至第3代.将第3代KC接种于培养瓶中,48 h后加入第3代MC(MC:KC为1:10)混合培养.制备人去表皮的真皮(DED),将培养第3代的MC、KC制成细胞悬液,按照1:10的比例接种于DED表面,采用液下培养和空气-液面培养相结合的方式进行培养,2周后取培养的组织工程皮肤分别做HE染色、角蛋白免疫组化染色及Masson-Fontana染色.结果 KC、MC混合培养于培养皿5 d后,于倒置显微镜下观察到KC呈铺路石状生长,其间散布MC,且树突延伸到KC细胞间.两种细胞混合接种于DED 15 d后,HE染色显示在DED上有3~6层表皮细胞,并可见角质层,角蛋白免疫组化染色阳性,银氨染色显示基底层见黑素着色.结论 MC、KC混合接种于培养皿可构建MC和KC接触生长的单细胞层,将MC、KC接种于DED可构建含有黑素成分的组织工程皮肤.Abstract: Objective To construct tissue-engineered skin containing melanin with mixed culture of human keratinocytes (KCs) and melanocytes (MCs) on de-epidermized dermis (DED) in vitro. Methods Single-cell suspension was obtained by digestion of isolated preputial epidermis with pancreatin. Keratinocyte serum-free medium (K-SFM) and modified M254 culture medium were used to culture KCs and MCs respectively. Third-passage KCs were seeded into cell culture flasks and cultured for 48 hours; then, third-passage MCs were seeded into the same cell culture flasks with the MC/KC ratio being 1: 10 followed by a 5-day coculture. The suspension of third-passage KCs and MCs with the MC/KC ratio of 10:1 were seeded onto the surface of a prepared DED and maintained at the air-liquid interface for 11 days following a 4-day submerged culture.Subsequently, the constructed tissue-engineered skin was examined with HE staining, immunohistochemical staining for keratin and Masson-Fontana staining. Results After coculture in flasks for 5 days, KCs exhibited a typical paving-stone appearance, MCs with projected dendrites were scattered in the extracellular space between KCs. HE staining revealed 3 to 6 layers of cells with the formation of stratum corneum after mixed culture on DED for 15 days. Keratin protein was positive throughout the artificial epidermis, and melanin pigments were located in the basal layer of the epidermis as Masson-Fontana staining showed. Conclusions The co-culture of MCs and KCs can form single-cell layers with the contact between MCs and KCs in flasks, and construct tissue-engineered skin with melanin component on DED in vitro. 相似文献
14.
目的研究p53蛋白在中波紫外线(ultraviolet lightB,UVB)诱导凋亡的富集表皮干细胞的角质形成细胞群中的表达情况。方法分离富集人表皮干细胞的角质形成细胞群和正常角质形成细胞群,使用UVB诱导两种细胞群凋亡,蛋白印迹法比较不同剂量UVB诱导前后两组细胞的p53蛋白表达的差异。结果两种细胞在不同剂量的UVB照射后p53蛋白表达均比照射前显著增加,在20mJ/cm2与40mJ/cm2照射剂量时,富集人表皮干细胞的角质形成细胞群p53蛋白表达高于正常角质形成的细胞群,差异有统计学意义(P<0.05)。结论富集人表皮干细胞的角质形成细胞p53蛋白表达比其它角质形成细胞对中波紫外线的照射易感。 相似文献
15.
16.
皮肤肿瘤干细胞与皮肤干细胞有一定的相似性,也有差异性.已经认识一些皮肤肿瘤干细胞表面的标记物,例如CD34、CD44、ABC家族成员、CD133、整合素、P63、CD20、角蛋白15、17和角蛋白19,以及与其相关的Wnt/β-连环蛋白/c-myc通路和Hedgehog/Shh等信号转导通路.探讨皮肤肿瘤干细胞特征性细胞表面标记与生物信号途径的关系,成为深入研究皮肤肿瘤干细胞的热点问题.阐明皮肤肿瘤干细胞表面标记及其信号通路与非肿瘤干细胞之间的异同,将完善皮肤肿瘤干细胞的致病机制,为研究皮肤肿瘤的发生发展、转移和有效根治皮肤肿瘤提供新的思路. 相似文献
17.
干细胞是存在于所有多细胞组织中,具有自我更新、增殖分化潜能的一种未分化细胞。干细胞的种类随研究的深入逐渐增多。干细胞治疗相关疾病的具体机制多数未明。目前在皮肤科领域,干细胞研究主要集中于皮肤损伤修复与组织工程学再生以及自身免疫相关性疾病、遗传性皮肤病、皮肤自然衰老与光老化、皮肤肿瘤等疾病治疗方面。干细胞应用的适应证随着研究的深入逐渐增加,特别在目前传统治疗方法棘手的自身免疫疾病、遗传性皮肤病、皮肤肿瘤等领域显示出优势。 相似文献
18.
皮肤肿瘤与其他组织肿瘤一样,存在着皮肤肿瘤干细胞,其来源可以来自皮肤干细胞,也可以来自分化成熟的角质形成细胞,还可以来源于皮肤外成体干细胞.皮肤肿瘤干细胞在体内有很强的肿瘤形成能力.皮肤肿瘤干细胞在肿瘤形成过程中牵涉到有关重要的分子和信号通路,有些分子和信号通路与正常组织不同.通过对这些通路和分子的调节,有可能找到治疗皮肤肿瘤的新途径. 相似文献