温热联合细胞周期特异性药物对恶性胸膜间皮瘤细胞的影响

通过体外实验研究温热联合细胞周期特异性药物羟基喜树碱（ＯＰＴ）对ＳＭＣ１ 恶性胸膜间皮瘤细胞株的影响。结果显示：经０１ μｇ／ｍ ｌ、１０ μｇ／ｍ ｌ、１００ μｇ／ｍ ｌＯＰＴ 处理４８ ｈ 对ＳＭＣ１ 细胞增殖有抑制作用,其抑制作用具有药物浓度依赖性,流式细胞仪测定显示经ＯＰＴ处理后细胞周期出现改变,Ｓ期细胞下降,Ｇ２＋ Ｍ 期细胞增加（Ｐ＜ ００５ 或Ｐ＜ ００１）;单独加温３９℃、４１℃、４３℃６０ ｍ ｉｎ 对ＳＭＣ１ 细胞增殖同样具有抑制作用,并有温度依赖性;温热联合ＯＰＴ 对ＳＭＣ１ 细胞增殖具有最佳抑制效果,该效果显现出温度药物浓度依赖性。该实验为临床应用提供了一定的理论依据     

胶束电动毛细管色谱法测定兔血浆中氟比洛芬的浓度

目的：建立胶束电动毛细管色谱法测定兔血浆中氟比洛芬的浓度。方法：兔血浆经甲醇除蛋白质后,加入０．１ ｍ ｌ分离用缓冲液溶解后进样测定。电泳条件为：７５ μｍ ×１００ ｃｍ 空心石英毛细管柱,缓冲液为２０ ｍ ｍ ｏｌ／Ｌ 硼砂（含２５ ｍ ｍ ｏｌ／Ｌ十二烷基硫酸钠）。结果：本法以酮基布洛芬为内标,在１～２４ μｇ／ｍ ｌ内呈良好的线性关系和重现性,２．４,７．２和１２．０ μｇ 的加样回收率分别为９５．３６％ ,９３．５５％ 和９５．２２％ ,最低检测浓度为０．２ μｇ／ｍ ｌ。结论：本法准确、简便,可用于药代动力学研究。     

阿奇霉素分散片人体生物利用度及生物等效性对比研究

以藤黄八迭球菌为指示菌,采用微生物法测定 １０ 名志愿者阿奇霉素分散片（实验制剂）和胶囊（参比制剂）经时血药浓度。最低检测限度 ０００５μｇ／ｍ ｌ,线性范围 ００１～０４μｇ／ｍ ｌ。实验采用双交叉单剂口服给药设计,数据经 ３ Ｐ９７ 药代动力学程序处理,阿奇霉素实验制剂和参比制剂主要药物动力学参数 ｋａ为０７２１９±０．０７５５ｈ－ １ 和 ０．７５５２±０．０５９３ｈ－ １,ｔ１／２（α）为 ２．３１８８±０．２４３８ｈ 和 ２．３５１８±０．３８１１ｈ,ｔ１／２ （β）为４１．５８１０ ±２． ２２８５ｈ 和 ４４．１２８３±９．８６４３ｈ, Ｃｍ ａｘ 为 ０．２４５８ ±０．００５８μｇ／ｍ ｌ 和 ０．２３８３±０．００６１μｇ／ｍ ｌ, Ａ Ｕ Ｃ０～１２０为 ０．４１７０±０．０８７４μｇ／（ｍ ｌ·ｈ ）和 ０．３８７７±０．０９６２μｇ／（ｍ ｌ·ｈ ）,实验制剂相对生物利用度为１０７．０５６６％ 。经双单侧ｔ检验和（１２α）置信区间法分析,两种制剂主要药代动力学参数剂型间、阶段间和个体间差异均无显著性（ Ｐ＞ ０．０５）,证实二者具有生物等效性。     

金银花黄芪合用抗Ⅱ型疱疹病毒药效分析

目的：获得抗 Ｈ Ｓ Ｖ 中药的最佳配方比。方法：以 Ａ Ｃ Ｖ 为阳性对照组,采用空斑减数实验,观察金银花与黄芪单用及合用抗 Ｈ Ｓ Ｖ ２３３３ 株的药效,用中效作用原理对药效进行了分析。结果：在 Ｈｅｐ ２ 细胞系统中, Ａ Ｃ Ｖ 对 Ｈ Ｓ Ｖ ２３３３ 株直接杀灭增殖抑制感染阻断的 Ｅ Ｄ５０分别为１６４５,１０８５, １８６２ μｇ／ｍ ｌ 合用比为 １（ Ｅ Ｄ５０）金银花／１（ Ｅ Ｄ５０）黄芪时,相应数值分别为 ０６７, ０８０,１１９ μｇ／ｍ ｌ,药效比 Ａ Ｃ Ｖ 强,而且合用指数（ Ｃ Ｉ）较小,协同作用十分明显。结论：上述抗 Ｈ Ｓ Ｖ ２３３３株合用比是组方时的最佳配方比。     

家兔血清中二氯乙酸钠浓度的测定

目的：建立用反相离子对色谱法测定家兔血清中游离二氯乙酸钠（ Ｄ Ｃ Ａ Ｎａ）浓度的方法。方法：用高效液相色谱法测定了家兔血清中 Ｄ Ｃ Ａ Ｎａ 的含量。 Ｄ Ｃ Ａ Ｎａ 在碱性条件下与水合肼定量生成稳定的腙,以 Ｉ Ｐ Ｒ Ｂ８为色谱试剂,在 Ｚｏｒｂａｓ Ｏ Ｄ Ｓ（４８ ｍ ｍ ×２５０ ｍ ｍ ）柱上,以水 甲醇 Ｉ Ｒ Ｐ Ｂ８为流动相进行人离。结果：分离灵敏度为００５ Ａ Ｕ Ｆ Ｓ,在２６８ ｎｍ 处,衍生物线性范围０６５～５００μｇ／ｍ ｌ,ｒ＝ ０９９９５,回收率（９８５±３３）％ 。结论：此方法为研究 Ｄ Ｃ Ａ Ｎａ 在人体内的药代动力学及药效学提供了参考。     

乙酰水杨酸－聚－α，β－DL－（3－羟丙基）－天冬酰胺共价复合体中乙酰水杨酸含量测定

作者采用比色测定法，建立了一种测定乙酰水杨酸－聚－α，β－ＤＬ－（３－羟丙基）－天冬酰胺（ＡＳＡ－ＰＨＰＡ）共价复合体中的乙酰水杨酸（ＡＳＡ）含量的方法。该法先将ＡＳＡ－ＰＨＰＡ经０．１ｍｏｌ／ＬＮａＯＨ水解生成水杨酸（ＳＡ）后，在ｐＨ２．５～３．５的缓冲溶液中，用ＦｅＣｌ３与ＳＡ生成络合物，检测波长为５２５ｎｍ，并进行比色测定，其线性范围在２～８０μｇ／ｍｌ，加样回收率为１００．１０±０．４４％，方法简便可行。     

O/W型甲苯咪唑口服乳剂的质量标准研究

目的：建立 Ｏ／ Ｗ 型甲苯咪唑乳剂的含量测定方法和质量标准。方法：采用紫外分光光度法测定 Ｏ／ Ｗ型甲苯咪唑乳剂的含量。结果：标准曲线为： Ｃ（μｇ／ｍ ｌ）＝ １９．９５ Ａ－ ０．３４３８,ｒ＝ ０．９９９９。线性范围为３．０～１６．０ μｇ／ｍ ｌ,平均回收率为（１００．０±０．１５）％ , Ｒ Ｓ Ｄ＝ ０．１５％ 。该方法的精密度和重现性分别为０．３６％ 和０．１７％ （ Ｒ Ｓ Ｄ％ ）。结论：用紫外分光度法测定乳剂中的甲苯咪唑含量,方法简便准确,样品无需分离即可直接测定,乳剂中的其它组份对测定无干扰。用紫外光谱法和薄层色谱法对乳剂中的甲苯咪唑进行鉴别,专属性强。     

苯那普利拉对高血压所致左心室重构及舒张顺应性变化的影响

目的：观察苯那普利拉（洛汀新）对高血压所致左心室重构及舒张顺应性变化的影响。方法：５０例高血压病伴左心室肥厚的患者,Ⅰ组（治疗组）３０例,给予洛汀新１０ ｍ ｇ／ｄ,Ⅱ组（对照组）２０例,给予硝苯吡啶３０ ｍ ｇ／ｄ,治疗６个月后,测定左心室室壁厚度及左心室舒张功能的变化。结果：治疗组血压由２３９４±４７９／１４２５±１３３ ｋ Ｐａ 降至１８３５±２１８／１２２１±１３４ ｋ Ｐａ（ Ｐ＜ ００１）,左室后壁厚度由１２５±２５ ｍ ｍ 降至１１２±２３ ｍ ｍ ,室间隔厚度由１２６±２８ ｍ ｍ 降至１１０±２２ ｍ ｍ （ Ｐ均＜ ００１）,二尖瓣前叶 Ｅ Ｆ 斜率（ Ｅ Ｆ Ｖ）由１２０±２３ ｍ ｍ ／ｓ 升至１５０±４０ ｍ ｍ ／ｓ, 左室舒张末压（ Ｌ Ｖ Ｅ Ｄ Ｐ）由１５７±０４１ ｋ Ｐａ 降至１３５±０２０ ｋ Ｐａ（ Ｐ 均＜ ００１）。而对照组,除血压有显著下降外（ Ｐ＜ ００１）,其他指标均无显著变化（ Ｐ 均＞ ００５）。结论：洛汀新不但有良好的降压效果,而且可逆转左室肥厚,抑制心肌重构,改善左室舒张顺应性。     

乙酰水杨酸—聚—α,β—DL—（3—羟丙基）—天冬酰胺共价复合体…

作者采用比色测定法，建立了一种测定乙酰水杨酸－聚－α，β－ＤＬ－（３－羟丙基）－天冬酰胺共价复合体中的乙酰水杨酸含量的方法。该法先将ＡＳＡ－ＰＨＰ经０．１ｍｏｌ／ＬＮａＯＨ水解生成水杨酸后，在ＰＨ２．５－３．５的缓冲溶缓中，用ＦｅＣｌ３与Ｓ生成络合物，检测波长为５２５ｎｍ，并进行比色测定，其线性范围在２－８０μｇ／ｍｌ，加样加收率为１００．１０±０．４４％方法简便可行。     

头孢克洛分散片在健康人体的药物动力学及相对生物利用度研究

研究头孢克洛分散片在健康人体内的药物动力学及相对生物利用度。采用高效液相色谱法测定８ 名志愿者单剂量口服５００ ｍ ｇ 头孢克洛分散片和胶囊后的血液浓度。药动学参数经３Ｐ８７ 软件处理计算。头孢克洛分散片的主要药动学参数：Ｃｍ ａｘ（１５３２±２４２） ｍ ｇ／Ｌ,Ｔｐｅａｋ（０５７±０１５） ｈ,Ｔ１／２ｋｅ（０７６±０２５） ｈ,ＡＵＣ（２４０７±４４７） ｍ ｇ／（ｈ·Ｌ）;头孢克洛胶囊的主要药动学参数：Ｃｍ ａｘ（１５．６３±２．３２） ｍ ｇ／Ｌ,Ｔｐｅａｋ（０．６０±０．１３） ｈ,Ｔ１／２ｋｅ（０６７±０１８） ｈ,ＡＵＣ（２３１３±３３７）ｍ ｇ／（ｈ·Ｌ）。经统计学处理,两种剂型的药动学参数之间差异均无显著性（Ｐ＞ ００５）。头孢克洛分散片的相对生物利用度为（１０３７５±１１３６）％ ,双单测ｔ检验（ＮＤＳＴ程序）的结果表明,两种制剂生物等效。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.