Clinical effect and action mechanism of Weicao Capsule (威草胶囊) in treating gout

Objective： To study the therapeutic effect of Weicao Capsule （威草胶囊, WCC） on gout. Methods： Two hundred gout patients were assigned to two groups. The treated group was treated with WCC and the control group was treated with Tongfengding Capsule （痛风定胶囊）. Both groups were given the respective treatments orally 3 times a day, 2 capsules each time with 2 weeks as one course and all patients received 2 successive courses of treatment. Changes of blood β2- microglobulin （13 2-M）, hemoglobin （Hb）, 24 h urinary protein （24 h UP）, pH value of urine and blood uric acid （BUA） as well as kidney function were observed. Results： After treatment, level of β 2-M got lowered significantly, Hb and 24 h UP, blood urea nitrogen, serum creatinine and the clearance rate of creatinine, as well as blood lipids all improved obviously in the treated group （all P〈0.01）, while these parameters remained unchanged in the control group （P〉0.05）. The pH value of urine was improved in both groups showing an insignificant difference between them （P〉0.05）. BUA was decreased in both groups with a decrease to a larger extent in the treated group （P〈0.01）. The total effective rate was 87% in the treated group, which was superior to that in the control group （62%, P〈0.05）. Conclusion： WCC has a favorable therapeutic effect on gout and its mechanism of action for improving renal function and reducing urinary protein could be related with the lowering of blood β 2-M, BUA and lipids.     

A Clinical Study of Suogudan Granule (索骨丹颗粒) in the Treatment of Rheumatoid Arthritis

Objective: To study the clinical efficacy of Suogudan Granule (SGDG, 索骨丹颗粒) in the treatment of rheumatoid arthritis (RA). Methods: Ninety patients with RA were randomly divided into the treated group and the control group. The treated group was administered orally with SGDG 6 g each time,thrice a day, while the control group with the combined therapy of Fenbid Capsules 0.3 g each time, twice a day and Tripterygium tablet 20 mg each time, thrice a day. The treatment course for both groups was 6 weeks. The changes of clinical symptoms and signs, and laboratory indices such as erythrocyte sedimentation rate (ESR), rheumatoid factor (RF), antistreptolysin O (ASO), routine examination of blood and urine,liver and kidney function, etc. before and after treatment were observed. Results: (1) The total effective rate in the treated group (88.0%) was obviously higher than that in the control group (67.5 %) with significant difference ( P＜0.05). (2) The improvement in arthralgia, joint swelling, time of morning stiffness, 15-meter walking, analgesia initiation and persistence in the treated group was better than that in the control group ( P＜0.05, P＜0.01), but there was no obvious difference in improvement of joint tenderness, range of joint motion, grip strength, and initiating detumescence time ( P＞0.05 ). (3) The improvement in ESR and RF in the treated group was better than that in the control group with significant difference ( P＜0.05). The negative-conversion rate of ASO in the treated group was also higher than that in the control group ( P＜0.01 ).(4) No evident abnormality in blood, urine, liver or kidney function was found in either group. Conclusion:SGDG is effective and safe for the treatment of RA.     

Effect of Angelica sinensis polysaccharide-iron complex on iron deficiency anemia in rats

Objective:To investigate the therapeutic effects of Angelica sinensis polysaccharide- iron complex(APIC)on rats with iron deficiency anemia(IDA).Methods:The IDA rat model was established by adopting low-iron forage with a small amount of regular bloodletting.The rats were randomly divided into a model group,three AIPC groups(high,middle,and low dosage),an Angelica sinensis polysaccharide(ASP)group,a mixture group(ASP FeCl_3)and a positive control group (Niferex).Changes in hemoglobin(Hb),red blood cell count(RBC),hematocrit(HCT)and iron content of whole blood were observed.Results:There was a significant difference before and after administration in all treated groups and all indices were restored to near-normal levels in the APIC groups and the positive control group.There was a significant difference among the changes of the indices in all the APIC groups and those of the model group but not between those of the APIC groups and the positive control group.However,the recovery of the indices in the APIC groups was superior to that in the positive control group.Conclusion:APIC not only has a superior therapeutic effect on IDA,but also has the effect of the ASP on supplementing blood and activating blood circulation.Hence,it may be used as a new iron-supplementing agent with a double therapeutic efficacy on blood supplementation for the treatment of IDA.     

Effects of Rebixiao Granules(热痹消颗粒剂) on Blood Uric Acid in Patients with Repeatedly Attacking Acute Gouty Arthritis

Objective:To observe the clinical effect of Rebixiao granule (热痹消颗粒剂, RBXG) in treating repeatedly attacking acute gouty arthritis and through experimental study on blood uric acid to explore RBXG's therapeutic mechanism. Methods: Ninety repeatedly attacking acute gouty arthritis patients were divided into the treated group (n= 60) and control group (n = 30). The treated group was treated with RBXG, and the control group was treated with Futalin tablets (diclofenac sodium). The baseline treatment including good rest, Iow purine diet, sufficient water drinking and urine alkalization, etc. was then given to both groups. Hypoxanthine 600 mg/kg and niacin 100 mg/kg was applied to hyperuricemic mice by gastrogavage to establish the animal models. Results: The clinical effective rate of the treated group was 95.0% and that of the control 90.0 %. Good therapeutic effects were won, insignificant difference (P＞0.05)was shown between the two groups. However, the cure rate of the treated group was 26.7% while that of the control group was 10.0%, with significant difference (P＜0.01) shown between them. The treated group had its blood uric acid lowered, which was significantly different (P＜0.05) from that of the control group. The animal experiment indicated that all the three groups treated with different dosages of RBXG, as well as the Ash bark and Smilax glabra rhizome groups had their blood uric acid content reduced in the hyperuricemic mice. Conclusion: RBXG has a quicker initiation and better treatment effects than sole anti-inflammatory and analgesic agents on the treatment of repeatedly attacking acute gouty arthritis, showing no obvious toxic or adverse reactions and therefore good for long-term administration and likely to be a safe TCM preparation to control the symptoms and reduce the onsets of repeatedly attacking of acute gouty arthritis. The animal experiment shows that both the compound preparation and part of the single ingredients in the recipe have the function of reducing blood uric acid. However, the compound recipe has better therapeutic effects, proving to be superior to single drugs.     

Clinical observation on chemical damage of nephron and the preventive and therapeutic effects of Baoshen Mixture (保肾合剂) on it

Objective:To observe the change of nephron damaged by chemotherapy and to evaluate the effect of Baoshen Mixture(保肾合剂,BSM) in protecting and treating damaged nephrons.Methods:Four hundred tumor patients with normal renal function and ready to receive chemotherapy were randomly assigned to two groups.Both groups received one cycle of chemotherapy program of 28-30 days with conventional hydratization,alkalization and chloridization.To the 200 cases in the treated group BSM was given orally thrice a day,150 mL every time for 15 successive days and the other 200 cases in the control group were treated by chemotherapy alone.The clinical efficacy was compared after treatment,and the changed condition of damaged nephrons were monitored dynamically and compared at different time points(the 3rd,7th,14th and 21st day after chemotherapy) by measuring the micro-globulin β2(β2-MG),albumin(Alb) and immunoglobulin G(IgG) levels in urine with radioimmunoassay(RIA).Results:(1) The effective rates in the treated group at the 4 time points of observation were all higher than those in the control group respectively(P<0.05 or P<0.01);(2) Less occurrence of abnormal β2-M,Alb and IgG levels on the 14th and 21st day in the treated group took place compared to that in the control group(P<0.01);(3) Urinary levels of β2-MG,Alb and IgG reached the peak on the 7th day in both groups,and then,they came down gradually and returned to the normal level on the 21st day.However,comparison between the two groups showed that all the three parameters in the treated group on day 3,14 and 21 were lower than the respective one at the corresponding time points in the control group(P<0.05 or P<0.01).Conclusion:The chemotherapy damage on nephron is regular in time,and reversible when treated suitably.TCM shows a marked effect in protecting and treating the damage on nephron caused by chemotherapy.     

改良补肾活血方对实验性骨关节炎兔子模型的控制代谢及抗氧化作用

Objective: To observe the metabolic, regulatory and anti-oxidative effects of modified Bushen Huoxue Decoction (BSHXD, 补肾活血方), a Chinese herbal medicine for Kidney (Shen)-reinforcement and blood-activation, on an osteoarthritis (OA) rabbit model. Methods: A rabbit model for knee joint OA was established by the classic Hulth''s method. The OA model rabbits were randomized into 5 groups: the model control group, the positive control group treated with glucosamine sulfate, and the three BSHXD treated groups treated respectively with low, moderate, and high doses of BSHXD. In addition, a normal control group and a sham-operated group were set up. Experimental animals were sacrificed after a 7-week treatment, and pathological changes in cartilaginous tissue were estimated using the Mankin criteria. Hydroxyproline (Hyp) and malonaldehyde (MDA) contents in blood serum and urine, as well as superoxide dismutase (SOD) activity and nitric oxide (NO) content in blood serum and knee joint synovial homogenates were detected. Results: Mankin scoring showed insignificant statistical differences between the various treatment groups (P>0.05), but all were better than the model control group (P<0.05). Serum and urinary contents of Hyp and MDA as well as serum and synovial levels of NO were significantly lower, but the SOD activity in blood serum and synovial tissue was higher in the BSHXD treated groups than in the model group (P<0.01); the effect of BSHXD was dose-dependent to some extent. Conclusion: The modified BSHXD shows an effect of improving cartilage metabolism in experimental rabbits with OA, and possesses osteo-chondric protective effects in antagonizing peroxidation injury.     

Effect of Yitangning Granule on Glycometabolism and Urinary Micro Albumin of Diabetes B Patients

One hundred and twenty patients with diabetes B that belong to the type of kidney deficiency andblood stasis were randomly divided into a treatment group treated with Yitangning Granule ( 益糖宁颗粒), a control group A treated with Jiangtangshu Capsule (降糖舒胶囊) and a control group B treated with Glurenorm Tablet (糖适平片). A better total effective rate was found in the treatment group, as compared with the two control groups, but there was no obvious difference. However, the treatment group was superior to the two control groups (P＜0.05 or P＜0.01) in the reduction of blood sugar and urinary micro albumin. It indicates that Yitangning Granule can improve the clinical symptoms and glycometabolism in patients with diabetes B and has a reverse effect on early diabetic nephropathy (DN).     

Effect of Tiaozhi Yanggan Decoction (调脂养肝汤) in treating patients with non-alcoholic fatty liver

Objective:To observe the clinical efficacy and safety of Tiaozhi Yanggan Decoction (调脂养肝汤,TZYGD)in treating non-alcoholic fatty liver.Methods:One hundred and thirty-eight patients were enrolled and randomized into two groups according to the random number table in a ratio of 3:1,with 8 cases eventually dropping out.The symptoms,signs,liver function markers, blood lipids,iconographic indices and clinical comprehensive efficacy after a 12-week treatment course were assessed in 101 patients treated with TZYGD in the treated group and 29 patients treated with Thiola in the control group.Results:The total effective rate in the treated group and the control group was 81.19% and 68.97%,respectively,showing a significant difference between the two groups with the former being significantly higher than the latter(P<0.05).Moreover,the improvements in the symptoms,signs,liver function,blood lipids and iconographic indices in the treated group were favorable with no serious adverse reactions.Conclusion:TZYGD is effective and highly safe in treating non-alcoholic fatty liver.     

Effect of Xuesaitong Soft Capsule(血塞通软胶囊)on Hemorrheology and in Auxiliarily Treating Patients with Acute Cerebral Infarction

Objective: To observe the therapeutic effect of Xuesaitong soft capsule(血塞通软胶囊,XST)and its effect on platelet counts, coagulation factor 1 (CF1) as well as hemorrheologic indexes in treating patients with acute cerebral infarction (ACI). Methods: Two hundred and four patients with ACI were assigned into two groups, the control group ( n = 96) and the treated group ( n = 108). They were all treated with conventional Western medicines, including mannitol, troxerutin, citicoline, piracetam and aspirin, while to the treated group, XST was given additionally through oral intake, twice a day, 2 capsules each time for 8successive weeks. The clinical efficacy was evaluated according to the nerve function deficits scoring and the changes of platelet count. CF1 and hemorrhe.ological indexes were measured before and after treatment. Results: The total effective rate was 87.0% in the treated group, and 87.5% in the control group, respectively, showing insignificant difference between them. But the markedly effective rate in the treated group (66.7%) was significantly higher than that in the control group (27.1%, P＜0.01). The count of platelet was not changed significantly in both groups after treatment, while CF1 inthem evidently lowered at the end of the 4th and 8th weeks of treatment, but showed insignificant difference between the two groups. The hematocrit, whole blood viscosity and plasma viscosity in both groups were all improved significantly after treatment, but also showed insignificant difference in comparison of the two groups. Conclusion: XST has good efficacy in auxiliary treatment of patients with ACI, though its mechanism remains to be further explored.     

Clinical Assessment on Treatment of Hyperlipidemia with Pushen Capsule (蒲参胶囊)

Objective: To explore the therapeutic effect of Pushen capsule (PSC, 蒲参胶囊) in treating primary hyperlipidemia. Methods: Two hundred and forty patients with primary hyperlipidemia were randomly divided into two groups, the 120 patients in the treated group treated with PSC (4 capsules, tid) and the 120 patients in the control group treated with Zhibituo tablet (脂必妥, 3 tablets, tid), and they were administered at the same time with Zhibituo placebo. The therapeutic course for both groups was 4 weeks. The therapeutic effect and the effects on blood lipids and viscosity were observed. Results: The effective rate in thetreated group was 76. 3%, which was significantly higher than that in the control group (48. 7% , P< 0. 01). PSC showed a significant lowering effect on TC, TG and LDL-C and raising effect on HDL-C, and the effect in lowering TG was significantly better than that of Zhibituo (P<0.01). PSC also showed a certain effect in decreasing whole blood viscosity of both high-sheared and low-shear     

Comparative Study on the Effects of Small Dose of Aspirin Combined with Ginkgo-damole Injection(银杏达莫注射液) and the Effects of Conventional Dose of Aspirin in Treating Senile Unstable Angina Pectoris

Objective: To evaluate the therapeutic and adverse effect of small dose of aspirin (Asp) combined with Ginkgo-damole injection (GDI,银杏达莫注射液) in treating senile unstable angina pectoris (UA). Methods: One hundred and twenty old in-patients of coronary heart disease with UA were randomly divided into two groups. The 60 patients in the treated group were treated with oral administration of enteric soluble 50 mg Asp once a day and GDI 20 ml per day by intravenous dripping, with 14 days as one therapeutic course, while the other 60 in the control group were treated with enteric soluble Asp 100 mg alone once a day orally. Besides, isosorbide-5-mononitrate 20 mg twice a day was applied to both groups, and the β-ad-renoceptor blocker, blood lipids regulatory agents and nitroglycerin (10 mg by intravenous dripping) were given accordingly. The angina total improving rate, hemorrheologic indexes (whole blood viscosity, plasma viscosity, fibrinogen, platelet aggregation rate), comprehensive clinical terminal     

Effects of Quyu Xiaoban Capsule(祛瘀消斑胶囊) on Vascular Endothelial Function in Patients with Atherosclerosis

Objective: To evaluate the therapeutic effects of Quyu Xiaoban Capsule (祛瘀消斑胶囊 ,QYXBC) on endothelial dependent vascular relaxation (EDVR) function in patients with atherosclerosis (AS)with ultrasonic technique. Methods: Tested were the endothelial function and blood lipids of 42 patients with AS in the treated group and 30 healthy volunteers in the control group. And re-examination of these parameters was carried out on the AS patients after they had been treated with QYXBC for 10 months. Results: Before treatment, the reactive hyperemia induced changes in artery diameter in the treated group was significantly lower than that in the control group (P＜0.01), while insignificant difference was found between the two groups in response to nitroglycerin. In the treated group after treatment, with D%-R improved significantly (P＜0.01), total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C)decreased by 16.3%, 5.6%, 10.2% respectively and high-density lipoprotein cholesterol (HDL-C) increased by 7.5%. EDVR was correlated negatively with the serum TC, LDL-C concentrations and the baseline brachial diameter (D0) (r = -0.41, -0.66, -0.59, respectively, all P＜0.01), but correlated positively with HDL-C (r = 0.62, P＜0.05). The ameliorative extent of EDVR was correlated positively to the decreased magnitude of TC and LDL-C concentrations ( r = 0.67,0.59, both P＜0.01 ). Conclusion: QYXBC can lower the level of blood lipids and improve significantly EDVR function.     

Effect of the principle of activating blood circulation to break stasis on GMP-140 and D2 dimer in patients with acute cerebral infarction

Objective:To explore the clinical efficacy of the principle of activating blood circulation to break stasis (ABCBS) and its influence on platelet membranous protein particle (GMP-140) and D2 dimer (Ddimer) before and after treatment. Methods: Eighty-eight patients with blood stasis syndrome (BSS) of acute cerebral infarction (ACI) were randomly divided into two groups, both of which were treated with conventional treatment, i.e. with western medicine (WM), with Salvia injection added through intravenously dripping.One of the two groups was used as the control and the other group as the treated group who had ABCBS herbs orally taken in addition. The duration of treatment course for both groups was 3 weeks. Results: There were changes in both groups over clinical symptoms, nerve function deficit scoring and GMP-140, D-dimer, but the treated group showed significantly better than that of the control group, (P＜0.05). Conclusion: ABCBS principle could serve as an important auxiliary treating method for BSS of ACI, as it can effectively alter the blood of ACI patients which was viscous, condense, coagulant and aggregating.     

Correlation between podocyte excretion and proteinuria in diabetic nephropathy patients

Objective： To observe the podocyte injury in diabetic nephropathy （DN） patients by identifying the urinary podocytes and the situation of detached podocytes in glomeruli and to demonstrate the correlation between podocyte excretion and proteinuria, blood glucose, serum creatinine in different phases in DN patients. Methods： Urinary podocytes and the podocalyxin （PCX） expression state of podocytes in glomeruli were identified and observed by indirect immunofluorescent method. The DN patients were divided into three groups according to the volume of proteinuria, namely small, medium and large volume proteinuria groups. The podocytes in the urine of every group were calculated. The DN patients were divided into five groups according to the chronic kidney disease （CKD） phases, then the positive podocytes in urine were calculated. Meanwhile, the 24-hour protein in urine, fasting blood glucose （FBG） and the serum creatinine of DN patients were tested. The correlations among the proteinuria, serum creatinine, FBG and the number of positive podocytes in the urine of DN patients were statistically analyzed. Results： Urinary positive podocytes were found in 88% of the patients with DN, whereas podocytes were found in 0% of patients with minimal changed disease （MCD） and healthy cases. The expression of PCX was absent in DN patients. In contrast, PCX was expressed integrally in MCD patients. The positive podocytes was 1.49±0.95/ml in small-volume proteinuria group, 2.15±0.70/ml in the medium-volume proteinuria group, and 3.48±1.27/ml in the large-volume proteinuria group. There was no significant difference between the small- and medium- volume proteinuria groups, and there were significant differences between other groups （P〈0.05）. The positive podocyte number tended to increase as proteinuria was increased. By Pearson analysis, the correlation between podocyte number and proteinuria was podocytes in urine from different groups of DN patients, CKD pc I sitive statistically. The difference of the number of positive -V group was significant statistically. The correlation between serum creatinine of CKD Ⅰ -Ⅲ group and positive podocytes in urine was positive statistically. The correlation between serumcreatinine of CKD Ⅳ- Ⅴ group and positive podocytes in urine was not significant statistically. The correlation between FBG and positive podocytes in urine was not significant either. Conclusion： The mechanism of the podocyte injury in DN patients is present. The podocyte injury in DN may positively correlate to proteinuria and serum creatinine of CKD Ⅰ -ⅢDN patients, but not to the FBG and serum creatinine of CKD Ⅳ-Ⅴ patients.     

The Midnight-Noon Ebb-Flow Point Selection for 30 Cases of Acute Ischemic Cerebrovascular Diseases

Objective:To observe the therapeutic effects of midnight-noon ebb-flow method of selecting acupoints (MNEFMSA) for acute ischemic cerebrovascular diseases(AICD)and its influence on hemorrheology and on the levels of interleukin-6(IL-6),thromboxane B2(TXB2)and 6-keto-prostaglandin F1α(6-keto-PGF1α).Methods:The 90 cases were randomly divided into 3 groups,each consisting of 30 cases.The drug group was treated mainly by routine medication;in addition to medication.the affected-channel group was treated by acupuncture at points along the course of the affected channel.and the MNEFMSA group was treated by MNEFMSA.Results:The total effective rate of MNEFMSA group.affected-channel group and drug group was 96.67%.90%and 73.33%respectively.The total effective rate of MNEFMSA group was obviously superior to that of the drug group(P＜0.01),and cure rate and marked improvement rate were obviously superior to those of the drug group and the affected-channel group(P＜0.05 or P＜0.01).After treatment,the three groups all got improvements in the hemorheological indexes.of which MNEFMSA group got marked improvements in the whole blood viscosity and erythrocyte deformability rate,significantly difierent from the other two groups(P＜0.05).At the early stage of treatment and after treatment,the three groups all had IL-6,TXB2 and 6-keto-PGF1α obviously improved(P＜0.05 or P＜0.01),of which MNEFMSA group got obvious improvement in 6-keto.PGF1α and IL-6 ever since the early stage of the treatment(P＜0.05).Conclusions:In the treatment of acute ischemic cerebrovascular diseases.MNEFMSA can markedly raise the clinical therapeutic effects by improving the hemorheological indexes,lowering the level of IL-6,and restoring the dynamic equilibrium between TXB2 and 6-keto-PGF1α,so as to promote the recovery of cerebral nervous function.     

Clinical study on effect of Astragalus Injection (黄芪注射液) and its immuno-regulation action in treating chronic aplastic anemia

Objective:To observe the clinical effect of Astragalus Injection(黄芪注射液,AI) and its immuno-regulatory action in treating chronic aplastic anemia(CAA).Methods:Sixty patients with CAA were randomly assigned to two groups equally,both were treated with Stanozolol three times a day,2 mg each time through oral intake,but AI was given additionally to the patients in the treated group once a day via intravenous dripping.All were treated for 15 days as one therapeutic course and the whole medication lasted for more than 4 months totally,with follow-up adopted.The clinical effi cacy was estimated and the changes of T-lymphocyte subsets in peripheral blood as well as the serum levels of tumor necrosis factor-α(TNF-α) and interleukin-2(IL-2) were observed.Results:The total effective rate in the treated group was 83.3%(25/30),which was higher than that in the control group 66.7%(20/30),showing significant difference between them(P<0.05).Levels of hemoglobin,WBC,reticular cell and platelet were elevated in both groups after treatment,but the improvement was signif icantly better in the treated group than that in the control group with respect to the former three indexes(P<0.05).The level of CD4 increased and that of CD8 decreased signifi cantly after treatment in the treated group(P<0.05),which showed significant difference as compared with those in the control group(P<0.05).Levels of serum TNF-α and IL-2 lowered after treatment in both groups,but signifi cance only showed in the treated group(P<0.05).The degree of proliferation in bone marrow got raised signifi cantly and the percentage of non-hemopoietic cells reduced signifi cantly in the treated group after treatment,also showing significant difference to those in the control group(P<0.05).Conclusion:AI could promote the recovery of hemopoietic function,which might be through improving T-lymphocyte subsets and reducing the release of negative regulatory factors such as TNF-α and IL-2 to alleviate the inhibition on hemopoietic function.     

Effect of Xin''''anning Nasal Drop ( 心安宁滴鼻剂 ) in Treating Coronary Heart Disease with Unstable Angina Pectoris

Objective: To observe the clinical efficacy of Xin'anning Nasal Drop (XAND, 心安宁滴鼻剂 )in treating coronary heart disease with unstable angina pectoris (CHD-UAP). Methods: Sixty patients with CHD-UAP were assigned, according to the randomizing number table, to two groups, the control group treated with conventional Western medicine, and the treated group treated with conventional Western medicine plus XAND. The clinical efficacy and the changes of S-T segment in resting EKG and total ischemia burden (TIB) in 24-h dynamic EKG were observed. Results: The clinical efficacy, including the effect of angina alleviation, its initiation, and the effect of TCM syndrome score reduction, were significantly superior in the treated group to those in the control group ( P＜0.05 or P＜0.01 ). The degree and extent of myocardial ischemia were significantly improved in both groups ( P＜0.01 ), but the improvement in the treated group was better than that in the control group ( P＜0.05). Moreover, it was worth mentioning that the immediate effect in the treated group was better than that in the control group, and the reduction of TIB, the improvement in heart rate and myocardial oxygen consumption (immediately after the first administration or by the end of the therapeutic course), and systolic blood pressure after treatment in the former were all superior to those in the latter, showing significant difference ( P＜0.05 or P＜0. 01 ). Conclusion: XAND has a quick effect in alleviating angina in patients with CHD-UAP, and it is worthy of further studies and spreading in clinical practice.     

Therapeutic Role of Tangshenkang Granule(糖肾康颗粒) in Rat Model with Diabetic Nephropathy

Objective: To evaluate the renal protective effect of Tangshenkang Granule(糖肾康颗粒) in a rat model of diabetic nephropathy(DN).Methods: Forty male Sprague-Dawley rats were randomly divided into control,DN,Tangshenkang and benazepril groups.DN model was established in the rats of DN,Tangshenkang and benazepril groups.Tangshenkang Granule solution and benazepril hydrochloride solution were intragastrically administered daily to the rats in the Tangshenkang and benazepril groups for 8 weeks,respectively.Urinary albumin and creatinine were detected.The albumin/creatinine(ACR) was calculated in addition to 24 h urinary protein(24-h UPr),serum creatinine(Scr),blood urea nitrogen(BUN),total cholesterol(TC),triglyceride(TG),low-density lipoprotein(LDL),high-density lipoprotein(HDL),and creatinine clearance rate(Ccr).Right kidneys were harvested for pathological observation using periodic acid-silver methenamine-Masson staining.The average glomerular diameter(DG),average glomerular(AG) and mesangial areas(AM) were measured.The thickness of glomerular basement membrane(TGBM) was detected using transmission electron microscope.Results: Compared with rats in the control group,rats in the DN group showed significantly decreased body weight,increased hypertrophy index,24-h urinary volume,24-h UPr,ACR,Scr,BUN,Ccr,blood lipids as well as renal pathological indices including DG,AG,AM,AM/AG and TGBM(P0.05).Compared with the DN group,the weights of rats in the Tangshenkang and benazepril groups were significantly increased,and the renal hypertrophy indices were significantly decreased(P 0.05).The 24-h urinary volumes,ACR,24-h UPr,Scr,BUN,Ccr,LDL,DG,AG,AM and TGBM were obviously decreased(P 0.05).Compared with the benazepril group,the Tangshenkang group showed significantly decreased levels of ACR,24-h UPr,AG and AM(P 0.05).Conclusion: Tangshenkang Granule decreased the urinary protein,attenuated the high glomerular filtration rate and improved lipid metabolism in DN rats,and prevented further injury induced by diabetic nephropathy.     

Clinical observation on the treatment of chronic urticaria with total glucosides of paeony capsule combined with citirizine

<正>Objective:To assess the effect and adverse reaction of total glucosides of paeony capsule(TGPC)in combining with citirizine for the treatment of chronic urticaria.Methods:A total of 120 patients were assigned to two groups by lottery,65 in the treated group and 55 in the control group.They all were orally treated with citirizine tablet 10 mg per day,but to the treated group,additional 0.2 g TGPC was given three times per day,the therapeutic course for both groups was 4 weeks.The effectiveness of treatment was observed,and the changes of total symptom score,serum levels of interleukin-4(IL-4),and immunoglobulin E(IgE)were measured before and after treatment.Moreover,a followup was carried out one month after ending the treatment.Results:The dropped cases were two in the treated group and seven in the control group;so,the study was accomplished on 63 patients in the treated group and 48 patients in the control group.The total effective rate was assessed at 73.02%(46/63)in the treated group,which was significantly higher than 47.92%(23/48)in the control group(P0.01).After treatment,the total symptom score decreased in both groups,but the decrement in the treated group was more significant(P0.05).Serum levels of IL-4 and IgE in the treated group lowered significantly,while the changes in the control group were insignificant,so statistical significant differences were shown between groups(P0.01).A follow-up study showed that the relapse rate in the treated group was 30.00% (6/20),while that in the control group was 90.00%(9/10),and the former was lower than the latter(P0.01).Adverse reactions,revealed as drowsiness,dizziness,and weakness,were seen in eight cases and seven cases in the two groups,respectively.Besides,mild diarrhea occurred in two cases of the treated group.Condusioiis:The treatment of TGPC combining citirizine shows definite curative effect in treating chronic urticaria,with low relapse rate and without evident adverse reaction.Its therapeutic effect might be realized by means of regulating patients' immune function. Besides,the medication should be continued for a rather long period to achieve the full effect.