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1.
Dorcas O. Moronkola Isiaka A. Ogunwande Tameka M. Walker William N. Setzer Isaac O. Oyewole 《Journal of natural medicines》2007,61(1):63-66
The composition of the essential oils of the leaves and flowers of Tithonia diversifolia (Hemsl) A. Gray, Mexican sunflower, are reported. The oils were obtained by hydrodistillation in an all-glass Clevenger-type apparatus and analyzed by GC–MS. The leaf oil was comprised of an abundance of α-pinene (32.9%), β-caryophyllene (20.8%), germacrene D (12.6%), β-pinene (10.9%) and 1, 8-cineole (9.1%). Germacrene D (20.3%), β-caryophyllene (20.1%) and bicyclogermacrene (8.0%) characterized the oil of the flower. A number of aliphatic fatty acids and a diterpenoid compound, sandaracopimaradiene, that were present in the flower, could not be detected in the leaf oil. 相似文献
2.
Michele D. A. Magina Eduardo M. Dalmarco Alberto WisniewskiJr. Edesio L. Simionatto Juliana B. Dalmarco Moacir G. Pizzolatti Inês M. C. Brighente 《Journal of natural medicines》2009,63(3):345-350
The essential oils of the leaves of Eugenia brasiliensis, Eugenia beaurepaireana, and Eugenia umbelliflora were analyzed by GC–MS. The major compounds found in the oil of E. brasiliensis were spathulenol (12.6%) and τ-cadinol (8.7%), of E. beaurepaireana were β-caryophyllene (8.0%) and bicyclogermacrene (7.2%), and of E. umbelliflora were viridiflorol (17.7%) and β-pinene (13.2%). These oils were assayed to determine their antibacterial activity against
Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. All of the oils analyzed showed antibacterial activity, ranging from moderate to strong, which was most accentuated for
the E. umbelliflora and E. brasiliensis oils, which strongly inhibited the growth of S. aureus giving values of MIC = 119.2 and 156.2 μg/mL, respectively. 相似文献
3.
Composition of the essential oil from the root of <Emphasis Type="Italic">Artemisia annua</Emphasis>
Divya Goel Richa Goel Vijender Singh Mohammed Ali Gopal Rao Mallavarapu Sushil Kumar 《Journal of natural medicines》2007,61(4):458-461
Upon hydrodistillation, dried roots of Artemisia annua L. cultivar Jwarharti gave a pleasantly fragrant essential oil with a yield of 0.25%. GC and GC–MS analyses of the oil enabled
the identification of 52 components representing 83.2% of the oil. The oil was rich in sesquiterpenes and oxygenated sesquiterpenes
and had cis-arteannuic alcohol (25.9%), (E)-β-farnesene (6.7%), β-maaliene (6.3%), β-caryophyllene (5.5%), caryophyllene oxide (4.4%) and 2-phenylbenzaldehyde (3.5%)
as its major components. The oil was found to possess considerable fumigant activity and ability to repel adult Tribolium castaneum beetles. 相似文献
4.
Twenty-eight samples of mericarps of Perilla frutescens var. frutescens were collected through fieldwork performed in Phongsali and Xieng Khouang provinces in northern Laos. No perilla samples
were collected from Savannakhet province in the south although more than 20 sites were investigated. Perilla plants are mostly
grown mixed with dry-paddy rice by slash-and-burn cultivation in Laos. The most popular local name for perilla mericarps in
the area was “Ma Nga Chan”. Weight of 1,000 grains and hardness of the mericarps were measured, and all mericarps were found
to be large (weight of 1,000 grains around 2 g) and soft (limit load weight under 300 g), which were preferred for culinary
use in Laos. The composition of the essential oils obtained from the herbaceous plants raised from the mericarps was divided
into five types, perillaketone, elemicine plus myristicine, shisofuran, piperitenon, and myristicine, and GC–MS analysis of
these Laotian perilla samples showed that they were similar to those of corresponding types of known Japanese perilla strains.
One of the shisofuran-type perilla contained large amounts of putative α-naginatene, which is likely to be an intermediate
of the biosynthesis of naginataketone. The farmers' indifference to the oil type of the leaf seems to leave Laotian perilla
as a good genetic resource for studies of the biosynthesis of oil compounds. 相似文献
5.
Qun Zhang Feng Zuo Norio Nakamura Chao-Mei Ma Masao Hattori 《Journal of natural medicines》2009,63(3):304-310
The metabolism of ganoderiol F (GF), a cytotoxic and antitumor triterpene from Ganoderma lucidum, by intestinal bacteria and its pharmacokinetics in rats were investigated by using liquid chromatography/mass spectrometry/mass
spectrometry (LC/MS/MS). GF was converted to ganodermatriol by anaerobic incubation with bacterial mixtures from rats and
humans. This metabolite was detected in rat feces, but not in plasma and urine, after oral administration of GF. The fate
of GF after oral (p.o.) and intravenous (i.v.) administration to rats was examined in pharmacokinetics studies. Plasma samples
pretreated by solid-phase extraction were quantified by HPLC/MS/MS over a GF concentration range of 1.25–100 ng/ml (S/N = 5).
The intra- and interday precision (CV%) was below 8% and accuracy was within the range of 95.9–103.6% for all samples. The
range of recovery ratios was 89.2–98.2%. After the administration of GF at 0.5 mg/kg i.v., the plasma concentrations of GF
quickly declined and the elimination half-life values (t
1/2α and t
1/2β) were about 2.4 and 34.8 min. On the other hand, the elimination half-life values (t
1/2α) after p.o. administration of GF at doses of 20 and 50 mg/kg were 14.4 and 143.3 min for the former, and 18.6 and 114.6 min
for the latter. The AUC0–t
value was 11.17 (ng/ml) h at a GF dose of 0.5 mg/kg i.v., but 49.4 and 111.6 (ng/ml) h at GF doses of 20 and 50 mg/kg p.o.,
respectively, indicating that the AUC0–t
value is proportional to the administered oral doses. The estimated absolute bioavailability of GF in rats was F = 0.105. 相似文献
6.
Nadia El Fakhar Zoubida Charrouf Bernadette Coddeville Yves Leroy Jean Claude Michalski Dominique Guillaume 《Journal of natural medicines》2007,61(4):375-380
Five new triterpene saponins, arganine L (1), O (2), P (3), Q (4) and R (5), were isolated from the barks of Argania spinosa (L.) Skeels. Arganines L-P and R are bidesmosidic saponins. The structures of 1–5 were elucidated as 3-O-[β-d-xylopyranosyl-(1–4)-β-d-glucuronopyranosyl]-28-O-[β-d-apiofuranosyl-(1–3)-β-d-xylopyranosyl-(1–4)-α-l-rhamnopyranosyl-(1–2)-α-l-arabinopyranosyl] bayogenin, 3-O-[β-d-xylopyranosyl-(1–4)-β-d-glucuronopyranosyl]-28-O-[β-d-xylopyranosyl-(1–4)-α-l-arabinopyranosyl] bayogenin, 3-O-[β-d-xylopyranosyl-(1–4)-β-d-glucuronopyranosyl]-28-O-[α-l-arabinopyranosyl] bayogenin, 3-O-[β-d-xylopyranosyl-(1–4)-β-d-glucuronopyranosyl] bayogenin, and 3-O-[β-d-apiofuranosyl-(1–4)-β-d-glucuronopyranosyl]-28-O-[β-d-xylopyranosyl-(1–4)-α-l-rhamnopyranosyl-(1–2)-α-l-arabinopyranosyl] bayogenin, respectively, mainly on the basis of their spectroscopic data. 相似文献
7.
The edible flower of Torenia fournieri Linden ex E. Fourn was found to possess potent antioxidative activity in a rat brain homogenate model. Bioassay-guided isolation
of the active compounds from a CH2Cl2–MeOH (1:1) extract led to the isolation of acteoside (1), luteolin-7-O-β-glucoside (2), apigenin-7-O-α-rhamnosyl-(1→6)-β-glucoside (3), and apigetrin (4). 相似文献
8.
Il Kyun Lee Do Hoon Kim Seung Young Lee Kyung Ran Kim Sang Un Choi Jong Ki Hong Jei Hyun Lee Young Hyun Park Kang Ro Lee 《Archives of pharmacal research》2008,31(12):1578-1583
The column chromatographic separation of the MeOH extract from the aerial parts of Prunella vulgaris var. lilacina Nakai led to the isolation of fifteen triterpenoic acids (2–6, 9–13, 16–20), four flavonoids (14, 21–23), four phenolics (7, 8, 15, 24), and a diterpene (1). Their structures were determined by spectroscopic methods to be trans-phytol (1), oleanic acid (2) ursolic acid (3), 2α,3α,19α-trihydroxyurs-12en-28oic acid (4), 2α,3α-dihydroxyurs-12en-28oic acid (5), maslinic acid (6), caffeic acid (7), phydroxy cinnamic acid (8), 2α,3α,19α,23-tetrahydroxyurs-12en-28oic acid (9), 2α,3α,23-trihydroxyurs-12en-28oic acid (10), 2α,3β-dihydroxyurs-12en-28oic acid (11), 2α,3β,24-trihydroxyolea-12en-28oic acid (12), (12R, 13S)-2α,3α,24,trihydroxy-12,13-cyclo-taraxer-14-en-28oic acid (13), quercertin 3-O-β-D-glucopyranoside (14), rosmarinic acid (15), 2α,3α,24-trihydroxyurs-12,20(30)-dien-28oic acid (16), 2α,3α,24-trihydroxyolea-12en-28oic acid (17), 2α,3β,19α,24-tetrahydroxyurs-12en-28oic acid 28-O-Dglucopyranoside (18), 2α,3α,19α,24-tetrahydroxyurs-12en-28oic acid 28-O-D-glucopyranoside (19), prunvuloside A (20), kaempferol 3-O-α-L-rhamnopyranosyl(1→6)-β-D-glucopranoside (21), kaempferol 3-O-β-D-glucopyranoside (22), quercertin 3-O-α-L-rhamnopyranosyl(1→6)-β-D-glucopyranoside (23), and 2-hydroxy-3-(3’,4’-dihydroxyphenly)propanoic acid (24). Compounds 1, 8–12, 17, 21, 23, and 24 were isolated from this plant source for the first time. The isolated compounds were evaluated for their cytotoxicity against
A549, SK-OV-3, SK-MEL-2, and HCT15 cells in vitro using the sulforhodamin B bioassay (SRB) method. Compound 3 exhibited moderate cytotoxic activity against A549, SK-OV-3, SK-MEL-2, and HCT15 cells, with ED50 values of 3.71, 3.65, 13.62, and 5.44 μM, respectively. 相似文献
9.
V. V. Vandyshev E. Yu. Babaeva D. D. Drozdovskaya 《Pharmaceutical Chemistry Journal》2009,43(3):154-156
Fatty oils extracted from the fruits of Echinacea purpurea and E. pallida species have been analyzed. The extracts represent oily and readily mobile light yellow liquids. Fruits of E. purpurea contain 33.6% fatty oil; of E. pallida, 23.2%. NMR spectroscopy analyses of fatty oils from fruits of the two Echinacea species show that these substances belong to liquid plant oils such as linoleic acid, which is confirmed by the measured
numerical indices.
Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 43, No. 3, pp. 32 – 34, March, 2009. 相似文献
10.
Hickey T Claffey J Fitzpatrick E Hogan M Pampillón C Tacke M 《Investigational new drugs》2007,25(5):425-433
Summary From the carbolithiation of 6-N,N-dimethylamino fulvene (3a) and different lithiated N-heterocyclic compounds (N,N-dimethylaminomethylpyrrole, 1-methylimidazole and 2,4-[bis(N′,N′-dimethylaminomethyl)]-N-methyl pyrrole), the corresponding lithium cyclopentadienide intermediate (4a–c) was formed. These three lithiated intermediates
underwent a transmetallation reaction with TiCl4 resulting in dimethylamino-functionalised titanocenes 5a–c. When these titanocenes were tested against LLC-PK cells, the
IC50 values obtained were of 13, and 63 μM for titanocenes 5b and 5c, respectively. The most cytotoxic titanocene in this paper
(5a) with an IC50 value of 6.8 μM is found to be almost as cytotoxic as cis-platin, which showed an IC50 value of 3.3 μM, when tested on the epithelial pig kidney LLC-PK cell line, and titanocene 5c is approximately 400 times
better than titanocene dichloride itself. 相似文献
11.
Toru Yamasaki Chikako Masuoka Toshihiro Nohara Masateru Ono 《Journal of natural medicines》2007,61(3):318-322
A new phenylethanoid glycoside, named acetyl forsythoside B (1), was isolated from the fruits of Callicarpa japonica Thunb. var. luxurians Rehd. (Verbenaceae) along with forsythoside B (2), brandioside (3), poliumoside (4), actioside (5), and apigenin 7-galacturonide (6). The structures of 1–6 were determined on the basis of spectroscopic data. In addition, the antioxidative activity of 1–4 and 6 was evaluated by the ferric thiocyanate method. All of the tested compounds except 6 exhibited almost the same activity as 3-tert-butyl-4-hydroxyanisole. The radical-scavenging effect of 1–6 on the stable free radical 1,1-diphenyl-2-picrylhydrazyl was also examined. Compounds 1–5 showed almost twice the activity compared to α-tocopherol. 相似文献
12.
Chemical investigation of the 80% Me2CO extract from the seeds of Prunus tomentosa led to the isolation and identification of six flavonoids: kaempferol (1), kaempferol 3-O-α-L-rhamnopyranoside (2; afzelin), kaempferol 3-O-β-D-(6-acetyl)-glucopyranosyl(1→4)-α-L-rhamnopyranoside (3; multiflorin A), kaempferol 3-O-β-D-glucopyranosyl(1→4)-α-L-rhamnopyranoside (4; multiflorin B), quercetin 3-O-α-L-rhamnopyranoside (5; quercitrin), and quercetin 3-O-β-D-glucopyranosyl (1→4)-α-L-rhamnopyranoside (6; multinoside A). Anti-oxidative and inhibitory activities on nitric oxide (NO) and prostaglandin E2 production in interferon-γ (INF-γ) and lipopolysaccharide (LPS)-activated RAW 264.7 cells in vitro (COX-2) of the isolated compounds were evaluated. Compounds 1, 5, and 6 exhibited potent anti-oxidative activity in the DPPH radical scavenging assay with IC50 values of 57.2, 59.4, and 54.3 μg/mL respectively. The positive control, ascorbic acid, had an IC50 of 55.5 μg/mL. Compounds 1, 5, and 6 also reduced COX-2 levels in a dose dependent manner with IC50 values of 10.2, 8.7, and 9.6 μg/mL respectively, with the positive control, indomethacin, having an IC50 of 5.1 μg/mL. All six compounds inhibited NO production in a dose dependent manner with IC50 values of 35.1, 42.8, 40.0, 44.8, 43.7, and 43.9 μg/mL respectively, while the positive control, L-NMMA, had an IC50 of 42.1 μg/mL. 相似文献
13.
Do Thi Ha Quan Cheng Chen Tran Manh Hung Ui Joung Youn Tran Minh Ngoc Phuong Thien Thuong Hong Jin Kim Yeon Hee Seong Byung Sun Min KiHwan Bae 《Archives of pharmacal research》2009,32(2):177-183
Chromatographic separation of the EtOAc fraction from the leaf and stem of Vitis amurensis led to the isolation of six oligostilbenoids (i.e., r-2-viniferin (1), trans-amurensin B (2), trans-ɛ-viniferin (3), gnetin H (4), amurensin G (5), (+)-ampelopsin A (8)) and four stilbenoids (i.e., trans-resveratrol (6), (+)-ampelopsin F (7), piceatannol (9), and trans-piceid (10)). The structures have been identified on the basis of spectroscopic evidence and physicochemical properties. The isolates
were investigated for cytotoxic activity against three cancer cell lines in vitro using the MTT assay method. Amurensin G (5) and trans-resveratrol (6) showed significant cytotoxic activity against L1210, K562 and HTC116 cancer cell lines with IC50 values ranging from 15.7 ± 2.1 to 30.9 ± 1.8 μM. (+)-Ampelopsin A (8) and trans-piceid (10) exhibited considerable cytotoxic activity against L1210 (IC50 values of 30.6 ± 4.1 and 28.7 ± 2.81 μM, respectively) and K562 (IC50 values of 38.6 ± 0.82 and 24.6 ± 0.76 μM, respectively). Gnetin H (4) showed only weak cytotoxic activity against L1210 with an IC50 value of 40.1 ± 4.23 μM. On the other hand, r-2-viniverin (1), trans-amurensin B (2), trans-ɛ-viniferin (3), (+)-ampelopsin F (7), and piceatannol (9) exhibited no activity on three cancer cell lines. 相似文献
14.
Huang J Tang XH Ikejima T Sun XJ Wang XB Xi RG Wu LJ 《Archives of pharmacal research》2008,31(3):323-329
A new triterpenoid, 20(R),22(ξ),24(S)-dammar-25(26)-ene-3β,6α,12β,20,22,24-hexanol (1), and three known triterpenoids, β-D-glucopyranoside,(3β,12β)-12,20-dihydroxydammar-24-en-3-yl,6-acetate (2), 20(R)-ginsenoside Rg3 (3), and 20(R)-ginsenoside Rh2 (4), were isolated from the leaves of Panax ginseng. Their structures were determined by chemical analysis and spectral methods (IR, 1D and 2D NMR, HR-ESI-MS). Compounds 1–4 were exhibited various degrees of cytotoxicity in the human hepatoma cell line, HepG2. Compound 1 had the highest cytotoxic potency, with an IC50 value of 20.1 μM, by stimulating p53-mediated cell cycle arrest at the G1 to S phase transition, leading to apoptosis via
activation of the caspase signaling pathway. 相似文献
15.
Objectives: Based upon reported ethnomedicinal use by Native Americans, extracts and pure isolates from leaves and seeds of Magnolia grandiflora, M. virginiana, M. acuminata and M. macrophylla, all native to the Southeastern United States, were investigated for their anti-inflammatory potential against cyclooxygenase
2 (COX-2).
Material and methods: The extracts and pure compounds from Magnolia species were tested for their production of prostaglandin E2 (PGE2) using a mouse macrophage (RAW 264.7) assay where cells were stimulated by lipopolysaccharide.
Results: Leaf extracts were moderately active (44–58% inhibition at 50 μg/ml) whereas seed extracts showed significant activity of
54–88% inhibition, respectively. In the seed extract of M. grandiflora, honokiol, magnolol and 4’-O-methylhonokiol strongly inhibited COX-2 (IC50: 1.2–2.0 μg/ml), 3-O-methylmagnolol was moderately active while a new compound was inactive towards COX-2. The neolignans were not cytotoxic to
macrophages (RAW 264.7) and kidney fibroblast (VERO) cells in vitro.
Conclusions: The results indicate that the reported ethnomedicinal use of the investigated Magnolia species is in agreement with anti-inflammatory activity of their respective compounds.
Received 8 December 2007; accepted 16 January 2009 相似文献
16.
A new ceramide, (2S,2′R,3R,4E,8E)-N-2′-hydroxyoctadecanoyl-2-amino-9-methyl-4,8-heptadecadiene-1,3-diol (1), was isolated together with four known sterols, 5α,6α-epoxy-3β-hydroxy-(22E)-ergosta-8(14),22-dien-7-one (2), ergosterol peroxide (3), cerevisterol (4) and 9α-hydroxycerevisterol (5), from the fruiting bodies of Ramaria botrytis (Pers.) Ricken (Ramariaceae). The structure of the new compound was elucidated based on spectral data. 相似文献
17.
V.F. Pres D.J. Moura A.R.M. Sperotto F.C. Damasceno E.B. Caramo C.A. Zini J. Saffi 《Food and chemical toxicology》2009,47(9):2389-2395
We have investigated the chemical composition of Piper gaudichaudianum essential oil, as well as its cytotoxic, mutagenic and genotoxic effects in V79 cells. The chemical analyses showed that the major compounds are (E)-nerolidol (22.4%), α-humulene (16.5%), (E)-caryophyllene (8.9%) and bicyclogermacrene (7.4%). Dose-dependent cytotoxic effects were observed in V79 cells treated with essential oil by using clonal survival, 3-(4,5-dimethylthiazole-2-yl)-2,5-biphenyl tetrazolium bromide reduction assay (MTT) and trypan blue exclusion assay (TB), and a significant decrease in survival was observed at concentrations of 0.5 μg/mL and higher. The P. gaudichaudianum essential oil treatment caused DNA strand breaks in V79 cells at concentrations up to 2 μg/mL, as detected by the alkaline comet assay, but did not induce double-strand breaks, as verified by neutral comet assay. It induced a significant increase in the frequency of micronucleated cells at 4, 6 and 10 μg/mL. Moreover, P. gaudichaudianum essential oil significantly increased lipid peroxidation at doses of 0.5 μg/mL and higher, suggesting that the observed oxidant potential can be responsible, at least in part, for its cytotoxic and genotoxic effects. 相似文献
18.
The dichloromethane extract of air-dried leaves of Blumea lacera (Asteraceae) afforded α-pinene-7β-O-β-d-2,6-diacetylglucopyranoside (1), 5,4′-dihydroxy-6,7,3′-trimethoxyflavone (2), and 3,5,4′-trihydroxy-6,7,3′-trimethoxyflavone (3). Compounds 1–3 showed moderate activity against Candida albicans, low activity against Trichophyton mentagrophytes, and were inactive against Aspergillus niger. Compounds 1 and 3 indicated low activity against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus and were inactive against Bacillus subtilis, while 2 was inactive against all four bacteria tested. 相似文献
19.
In this study, we investigated the hypoglycaemic and antidiabetic properties of mollic acid glucoside (MAG), a 1α-hydroxycycloartenoid
extractive from Combretum molle leaf, in rodents. Stepwise, escalated doses of MAG (5–80 mg/kg p.o.) produced dose-dependent and significant (P < 0.05–0.01) hypoglycaemic and antidiabetic effects in normal (normoglycaemic) and streptozotocin-treated diabetic rats.
Experimental evidence obtained from this laboratory animal study indicates that MAG, an extractive from C. molle leaf, possesses hypoglycaemic and antidiabetic properties. These findings lend pharmacological credence to the folkloric,
ethnomedical uses of the plant’s leaf in the management and/or control of diabetes mellitus in some rural communities of southern
Africa. 相似文献
20.
James W. Gathirwa Geoffrey M. Rukunga Eliud N. M. Njagi Sabah A. Omar Anastasia N. Guantai Charles N. Muthaura Peter G. Mwitari Cecilia W. Kimani Peter G. Kirira Festus M. Tolo Teresia N. Ndunda Isaiah O. Ndiege 《Journal of natural medicines》2007,61(3):261-268
Extracts of seven medicinal plant species used for treatment of malaria in traditional/cultural health systems of the Ameru
people in Kenya were tested in vitro and in vivo against Plasmodium falciparum (D6 and W2 strains) and P. berghei, respectively. Of the plants tested, 28.57% were highly active (IC50 <10 μg/ml) and 42.86% moderately active (IC50 10–50 μg/ml), while 28.57% had weak activity of 50–125 μg/ml in vitro. The water and methanol extracts of Boscia salicifolia Oliv. and Artemisia afra Jacq. (ex-Willd.) were the most active against both the chloroquine (CQ)-sensitive (D6) and the CQ-resistant (W2) P. falciparum strains. Artemisia afra and Rhus natalensis Bernh. (ex-Krauss) exhibited the highest parasite clearance and chemo-suppression (>70%) in vivo (in mice). The plants with
high in vitro anti-plasmodial (low IC50 values) and high anti-malarial activity (high chemo-suppression) in vivo are potential sources of novel anti-malarial drugs. 相似文献