血小板激活因子受体mRNA在小鼠附睾精子内的表达

目的 检测血小板激活因子受体（ＰＡＦＲ）ｍＲＮＡ在小鼠附睾精子内的表达,探讨ＰＡＦＲ对受精过程的影响。２方法 分离提高小鼠附睾精子ｍＲＮＡ,经逆转录（ＲＴ）合成ＰＡＦＲ－ｃＤＮＡ。利用聚合酶链反应（ＰＣＲ）扩增ＰＡＦＲ－ｃＤＮＡ,ＤＮＡ序列分析验证扩增产物的准确性。结果 小鼠附睾精子表达ＰＡＦＲ－ｍＲＮＡ;ＤＮＡ序列分析验证ＰＣＲ产物的小鼠ＰＡＦＲ－ｃＤＮＡ。④结论 小鼠附睾精子有ＰＡＦＲ－ｍＲＮ     

RT-PCR法测定脂肪组织解偶联蛋白mRNA表达水平的探讨

目的：建立解偶联蛋白（ＵＣＰ）ｍＲＮＡ表达水平的竞争ＲＴ－ＰＣＲ测定法;并应用该方法测定ＵＣＰ ｍＲＮＡ在成人腹膜内与腹膜外脂肪组织的表达水平。方法：应用剪接重叠延伸ＰＣＲ（ＳＯＥ－ＰＣＲ）合成中央缺失型ＵＣＰ竞争者ＲＮＡ。ＵＣＰ ｍＲＮＡ与作为内设标准的ＵＣＰ竞争者ＲＮＡ用相同的引物在同一反应体系中进行逆转录反应和ＰＣＲ扩增。通过比较ＵＣＰ ｍＲＮＡ产物和ＵＣＰ竞争者ＲＮＡ产物的放射性信号强度求     

食管癌Ⅰ型纤溶酶原激活物抑制剂的表达研究

目的：探讨食管癌组织中Ⅰ行溶酶原激活物抑制剂（ＰＡⅠ－１）的表达特征。方法：采用定量的逆转录聚合酶链反应（ＲＴ－ＰＣＲ）方法检测基因的表达。结果：食管癌组织中ＰＡⅠ－１的ｍＲＮＡ的表达水平显著高于正常粘膜组织（Ｐ〈０．０１）,组织原位杂交显示ＰＡⅠ－１的ｍＲＮＡ主要分布于肿瘤细胞的胞浆中。结论：ＲＡⅠ－１的ｍＲＮＡ在食管癌中的表达上调,ＲＴ－ＰＣＲ的定量分析可用于肿瘤诊断的辅助方法。     

半定量RT-PCR检测少量兔血管内皮细胞中特异mRNA的表达

目的：半定量检测体外培养的少量兔血管内皮细胞中特异ｍＲＮＡ的表达。方法：用Ｐｈａｒｍａｃｉａ Ｂｉｏｔｅｃｈ公司的快速微量ｍＲＮＡ提纯试剂盒从兔内皮细胞中直接提取出ｍＲＮＡ,并以此为模板,用半定量ＲＴ－ＰＣＲ技术检测培养的兔血管内皮细胞中内皮素（ＥＴ－１）ｍＲＮＡ的相对含量。结果：从１０＾５个兔血管内皮细胞中提取出未降解的ｍＲＮＡ,Ａ２６０／Ａ２８０〉１．８;用半定量ＲＴ－ＰＣＲ技术,测定出ＥＴ－     

幽门螺杆菌感染者胃粘膜中白介素—8mRNA的竞争RT—PCR检测

目的：从分子水平探讨幽门螺杆菌（Ｈｅｌｉｃｏｂａｃｔｅｒｐｙｒｏｌｉ，ＨＰ）感染对细胞因子ＩＬ－８ｍＲＮＡ表达的影响，说明竞争ＲＴ－ＰＣＲ法是定量细胞因子的精确方法。方法：３５例幽门螺杆菌感染患者，抗菌治疗前后分别取胃窦部活检粘膜２块，经逆转录反应合成ｃＤＮＡ，经竞争ＲＴ－ＰＣＲ法测定ＩＬ－８ｍＲＮＡ表达量，按配对ｔ检验处理数据。结果：根除成功者２８例，粘膜中ＩＬ－８ｍＲＮＡ的表达量显著下降（Ｐ＜０．０１），而根除失败者７例，粘膜中ＩＬ－８ｍＲＮＡ表达量无明显变化（Ｐ＞０．０５）。结论：ＨＰ感染可诱导粘膜中产生过量的细胞因子ＩＬ－８，竞争ＲＴ－ＰＣＲ法是定量细胞因子的精确方法。     

9-顺维甲酸对人肺腺癌细胞维甲酸受体转录的影响

目的：观察９－顺维甲酸（９－ｃｉｓ－ＲＡ）处理前后人肺腺癌细胞株ＰＧ和Ａ５４９维甲酸受体（ＲＡＲｓ）及维甲类Ｘ受体　（ＲＸＲｓ）不同时相ｍＲＮＡ转录水平的表达。方法：用ＲＴ－ＰＣＲ相对定量检测人肺腺癌细胞株ＰＧ和Ａ５４９加９－ｃｉｓ－ＲＡ前后ＲＡＲｓ和ＲＸＲｓ　ｍＲＮＡ转录水平。结果：应用９－ｃｉｓ－ＲＡ后，两细胞株的ＲＡＲα和ＲＡＲβ－ｍＲＮＡ转录水平较对照组明显增高，其余受体转录无明显变化。结论：受体ＲＡＲα和ＲＡＲβ可能与９－ｃｉｓ－ＲＡ对两细胞株的生长抑制和诱导凋亡作用有密切关系。     

RT-PCR法测定胎鼠大脑皮质内皮素-1 Mrna

目的 建立一种检测先天性人巨细胞病毒感染胎鼠大脑皮质内皮素－１（ＥＴ－１）ｍＲＮＡ的方法。方法 采用逆转录－聚合酶链式反应（ＲＴ－ＰＣＲ）方法并对实验条件进行优化。建立稳定的检测体系后,对胎鼠大脑皮质ＥＴ－１ ｍＲＮＡ进行检测,并以ＲＴ－ＰＣＲ后产物的ＯＤ值来反映起初ＥＴ－１ ｍＲＮＡ的模板相对含量。结果 在一定ＴａｐＤＮＡ酶、镁离子浓度、引物浓度等条件下,ＥＴ－１ ｍＲＮＡ经ＲＴ－ＰＣＲ扩增后产     

肝癌外周血AFP mRNA的测定及意义

目的 建立一种灵敏的巢式逆转录聚合酶链反应法（ＲＴ－ＰＣＲ）,用于检测外周血中的ＡＦＰ ｍＲＮＡ。方法 将患者分成四组：原发性肝细胞癌组３８例,慢性肝炎组９例,肝炎后肝硬化组１２例,正常对照组１０例。通过采肝素抗凝外周血、分离单个核细胞、抽提ＲＮＡ、逆转录合成ｃＤＮＡ、设计ＡＦＰ基因引物来进行巢式ＰＣＲ扩增。 结果 所有正常人外周血中ＡＦＰｍＲＮＡ全部阴性,原发性肝细胞癌组的外周血中ＡＦＰ ｍＲＮ     

uPAR mRNA测定及其在U937细胞中的表达

目的 研究凝血酶、ＴＮＦ－α、ＩＦＮ－γ等对Ｕ９３７细胞表达ｕＰＡＲ ｍＲＮＡ的影响,观察凝血系统、炎症介质与纤溶功能变化间的关系。 方法 建立并应用逆转录ＰＣＲ（ＲＴ－ＰＣＲ）法测定培养Ｕ９３７细胞的ｕＰＡＲ ｍＲＮＡ。 结果：１．经测定批间变异系数（ＣＶ间）确认了ＲＴ－ＰＣＲ法测定ｕＰＡＲ ｍＲＮＡ的稳定性;２．以不同浓度凝血酶刺激２４ｈ,对Ｕ９３７细胞表达ｕＰＡＲ ｍＲＮＡ有抑制和促进两种不     

急性髓系白血病H—ras,凋亡及多药耐药的研究

目的 研究急性髓系白血病（ＡＭＬ）Ｈ－ｒａｓ／Ｐ２１,抗凋亡基因、细胞凋亡与多药耐药基因的疗效的影响,并证实流式细胞术检测Ｐ－ｇｐ和逆转录多聚酶链反应（ＲＴ－ＰＣＲ）检测ｍｄｒ－１ ｍＲＮＡ间的相关。方法 用ＦＣＭ检测８５例初始ＡＭＬ患者的Ｈ－ｒａｓ／Ｐ２１、ｂｃｌ－２,细胞凋亡和ｍｄｒ－ｌ产物Ｐ－ｇｐ的表达;用ＲＴ－ＰＣＲ检测其中４７例的ｍｄｒ－ｌ／ｍＲＮＡ。结果 Ｐ２１＾＋／Ｐ－ｇｐ＾＋者完全     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.