Evaluation of plasma H2S levels and H2S synthesis in streptozotocin induced Type-2 diabetes-an experimental study based on Swietenia macrophylla seeds

ObjectiveTo evaluate the plasma H₂S levels and H₂S synthesis activity in streptozotocin induced type 2 diabetes rats compared to the healthy controls and also to observe the effect of the aqueous extract of Swietenia macrophylla (S. macrophylla) seeds on the experimental groups.MethodsSeeds of S. macrophylla were separated, washed, shed-dried and finally extract was prepared. Thirty two wistar rats were selected for the experimental study. Streptozotocin was used for the induction of diabetes. H₂S concentration in plasma was measured. H₂S synthesizing activity in plasma was measured. Statistical analysis have done using Microsoft excel, Office 2003. Values were expressed by mean ± SD. P<0.05 were considered statistically significant.ResultsFasting blood glucose level (7.74 ± 0.02) mmol/L was significantly increased in diabetic rats. The glucose levels are significantly lowered in the rats treated with metformin (5.48 ± 0.03) mmol/L as well as with aqueous extract of S. macrophylla seeds (3.72 ± 0.04) mmol/L. The HbA1c percentages in different groups of study subjects also indicate similar trends. Our study shows both the plasma H₂S levels (22.07 ± 0.73) mmol/L and plasma H₂S synthesis activity (0.411 ± 0.005 mmol/100 g) are significantly reduced in the streptozotocin induced diabetic rats.ConclusionsAlthough considering a small sample size, it can conclude that the fasting blood glucose levels are inversely related to plasma H₂S levels as well as H₂S synthesis activity in plasma and the extract of S. macrophylla is associated with increased plasma H₂S levels with effective lowering of blood glucose in streptozotocin induced diabetic rats.     

NANOG Alleviates the Damage of Human Hair Follicle Mesenchymal Stem Cells Caused by H_2O_2 through Activation of AKT Pathway

Objective To explore the protective effect of NANOG against hydrogen peroxide(H_2O_2)-induced cell damage in the human hair follicle mesenchymal stem cells(hHF-MSCs). Methods NANOG was expressed from a lentiviral vector, pLVX-IRES-ZsGreen. NANOG hHF-MSCs and vector hHF-MSCs were treated with 400 μmol/L hydrogen peroxide(H_2O_2) for 2 h, the cell survival rate, cell morphology, ROS production, apoptosis and expression of AKT, ERK, and p21 were determined and compared. Results Our results showed that NANOG could activate AKT and upregulate the expression of p-AKT, but not p-ERK. When treated with 400 μmol/L H_2O_2, NANOG hHF-MSCs showed higher cell survival rate, lower ROS production and apoptosis, higher expression of p-AKT, higher ratio of p-AKT/AKT. Conclusion Our results suggest that NANOG could protect hHF-MSCs against cell damage caused by H_2O_2 through activating AKT signaling pathway.     

Suppressive effects of genomic imprinted gene PEG10 on hydrogen peroxide-induced apoptosis in L0<Subscript>2</Subscript> cells

The effects of PEG10 on hydrogen peroxide （H2O2）-induced apoptosis in human normal liver cell line L02 were investigated. The PEG10 gene was transfected into L02 cells by lipofectamine, the positive clone was screened by G418 and defined as L02/PEG10, while the cell transfected with empty expression vector （pEGFP-N1） was defined as L02/vector. L02/vector and parental L02 cells served as control. RT-PCR and Western blotting were employed to detect the expression of target genes. H2O2 （50–400 mmol/L） was administered to induce the apoptosis of L02 cells. Cells viability was measured by MTT and the morphological changes of apoptotic cells were determined by fluorescence microscopy using hoechst33342 nuclei staining. DNA fragmentation was observed by agarose gel electrophoresis. PEG10 mRNA and protein levels in L02/PEG10 cells were significantly increased as compared with those in the control cells. After treatment with 400 mmol/L H2O2 for 24 h, the cellular growth inhibition rate of L02/PEG10 cells was significantly lower （58.2%） than that of L02 （92.5%） and L02/vector （88%）. Distinct morphological changes characteristic of cell apoptosis such as karyopyknosis and conglomeration were not observed in L02/PEG10. Ladder-like DNA fragmentation in a dose-dependent manner was observed in both L02 and L02/vector cell lines, but not in L02/PEG10. PEG10 over-expression significantly inhibited cytotoxicity induced by H2O2 on human normal liver cell line L02 by antagonizing H2O2-induced apoptosis.     

Hepatoprotective and Antioxidant Activity of Rhizome of Podophyllum hexandrum against Carbon Tetra Chloride Induced Hepatotoxicity in Rats

Objective To test possible antioxidant activity of n-hexane extract of Podophyllum hexandrum under in vitro and in vivo conditions. Methods The in vitro antioxidant activity was evaluated by the ability of the extract to interact with the stable free radical DPPH, Superoxide (O2-), Hydroxyl (OH-), Hydrogen peroxide (H2O2) radicals, and reducing power ability of the extract was also evaluated. Under in vivo conditions the extract was evaluated for its hepatoprotective activity by measuring different biochemical parameters, such as serum alanine aminotransaminase, serum aspartate aminotransaminase and serum lactate dehydrogenase and antioxidant enzymes. Antioxidant status was estimated by determining the activities of antioxidative enzymes, glutathione reductase (GR), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and superoxide dismutase (SOD), and by determining the levels of reduced glutathione (GSH) and thiobarbituric acid reactive substances (TBARS). Results Hexane extract of P. hexandrum exhibited good radical scavenging capacity in neutralization of DPPH, O2-, OH -, and H2O2 radicals in a dose dependent manner. n-hexane extract of Podophyllum hexandrum at the doses of 20, 30, and 50 mg/kg-day produced hepatoprotective effect by decreasing the activity of serum marker enzymes, while it significantly increased the levels of glutathione (GSH), glutathione peroxidase (GPx), glutathione reductase (GR), super oxide dismutase (SOD), and glutathione-S-transferase (GST) in a dose dependant manner. The effect of n-hexane extract was comparable to that of standard antioxidant vitamin E. Conclusion The extract of Podophyllum hexandrum possess free radical scavenging activity under in vitro conditions and could protect the liver tissue against CCl 4 induced oxidative stress probably by increasing antioxidant defense activities.     

O3/UV和O3/H2O2氧化法处理聚丙烯酰胺采油废水

为降低油田采油废水的化学需氧量（COD）负荷,提高其可生化性,采用O₃/UV和O₃/H₂O₂氧化法对聚丙烯酰胺采油废水进行处理,分别考察了氧化时间、H₂O₂与O₃物质的量之比、pH以及紫外灯功率对采油废水处理效果的影响。结果表明,与采用单独臭氧氧化相比,O₃/UV以及O₃/H₂O₂联用技术对采油废水中的COD及聚丙烯酰胺（PAM）的去除效果更为显著,废水可生化性均有所提高,且O₃/H₂O₂氧化法对采油废水的可生化性提高程度更大。O₃/UV氧化法对于聚丙烯酰胺采油废水可生化性影响的最佳条件为：pH=8.0,O₃质量浓度为19.7 mg/L,紫外灯功率为18 W,氧化时间为30 min,可生化性（B/C）提高至0.092;O₃/H₂O₂氧化法对于聚丙烯酰胺采油废水可生化性影响的最佳条件为：pH=8.0,O₃质量浓度为19.7 mg/L,H₂O₂与O₃物质的量之比为0.3,氧化时间为30 min,B/C提高至0.175。氧化预处理提高了废水的可生化性,减轻了后续生化处理压力。     

单细胞凝胶电泳分析地黄提取物对仓鼠肺成纤维细胞DNA的损伤作用

目的 采用单细胞凝胶电泳（彗星实验）技术研究地黄提取物致仓鼠肺成纤维细胞（CHL细胞）DNA的损伤作用,为该药物临床前安全性评价提供参考。方法 采用0.2、1.0、5.0 mg/mL地黄提取物,用双氧水作为阳性对照药,处理细胞,24 h后收获细胞,进行彗星电泳实验。结果/b> 双氧水处理后造成CHL细胞产生明显的DNA损伤,呈现不同彗尾长度的彗星,与阴性对照组比较有显著差异。不同质量浓度的地黄提取物处理细胞后,彗星长、彗尾长度等各项指标与阴性对照组比较无显著差异。结论 单细胞凝胶电泳检测DNA损伤的敏感度较高,不同质量浓度的地黄提取物对CHL细胞未产生DNA损伤。     

氨甲环酸对体外人脐静脉内皮细胞损伤的保护作用

目的 研究氨甲环酸（tranexamic acid, TXA）对H₂O₂诱导人脐静脉内皮细胞（human umbilical vein endothelial cells, HUVECs）损伤的保护作用及给药时机选择。方法 体外培养HUVECs,加入100μmol/L的H₂O₂作用12h,在H₂O₂作用开始后30min和60min以及H₂O₂作用结束前1h和2h这4个时间点加入TXA （150μmol/L）;Hoechst 33258染色法观察HUVECs形态;Western blot法检测细胞内细胞间黏附分子（intercellular cell adhesion molecule, ICAM）的表达;酶联免疫吸附双抗体夹心法（ELISA） 检测细胞上清液多配体聚糖、tPA和PAI-1含量。结果 HUVECs经H₂O₂作用后,可显著诱导细胞凋亡,上调ICAM、多配体聚糖和tPA的表达,同时抑制PAI-1的表达;TXA在H₂O₂作用60min内给药可抑制H₂O₂对HUVECs的凋亡诱导作用,同时下调ICAM、多配体聚糖和tPA的表达,并上调PAI-1的表达,TXA在60min后给药无上述保护作用。结论 早期TXA给药对H₂O₂诱导HUVECs损伤有明显抗纤维蛋白溶解和保护作用,从而表明临床应用TXA需早期给药。     

Chemical composition and antioxidant activity of a Lebanese plant Euphorbia macroclada schyzoceras

Objective

To determine the chemical composition, total phenolic and total flavonoid contents of the crude extracts from leaves and stems of a Lebanese plant Euphorbia macroclada schyzoceras (E. macroclada), and to evaluate their antioxidant potential using DPPH, H₂O₂, and chelating of ferrous ions tests.

Methods

Quantification of the total phenolic and total flavonoid contents of the crude extracts from leaves and stems and the antioxidant activities were evaluated using spectrophotometric analyses. The chemical composition has been estimated using different techniques such as IR, LC/MS and NMR.

Results

Ethanolic extract from leaves of E. macroclada was better than aqueous extract and showed higher content in total phenolic and total flavonoid than found in the stems. On the other hand, using DPPH and H₂O₂ tests, this extract from leaves showed higher antioxidant capacity than aqueous extract. However, using the chelating of ferrous ions test, the antioxidant activity of the aqueous extract of both stems and leaves was stronger than that of ethanolic once. The chemical composition of the whole plant showed the presence of some aromatic compounds and fatty acids.

Conclusions

Both ethanolic and water extracts from both parts of this plant are effective and have good antioxidant power. So, this plant can be used in the prevention of a number of diseases related to oxidative stress.     

载有人心肌细胞钠离子通道及PRKAG2基因的HEK-293T细胞模型的建立

目的尝试建立类似PRKAG2心脏综合征钠离子通道功能的HEK-293T细胞模型,为探讨PRKAG2心脏综合征心律失常机制奠定基础。方法分别构建人PRKAG2基因质粒载体和SCN5A基因质粒载体,共转人胚肾细胞(HEK-293T);应用免疫荧光法、Real-time PCR、蛋白质印迹法检测HEK-293T细胞转染结果及基因表达情况。结果转染48h后,HEK-293T细胞在细胞免疫荧光显微镜下可见红色荧光和绿色荧光。Real-time PCR及蛋白印迹结果证实:单独转染SCN5A组、SCN5A+PRKAG2组、SCN5A+G100S组、SCN5A+R302Q组SCN5A基因及蛋白均有表达,而在空白对照组无表达,差异有统计学意义(P<0.05);SCN5A+PRKAG2组PRKAG2基因及蛋白高表达,而空白对照组、SCN5A组、SCN5A+R302Q组、SCN5A+G100S组表达量较低,差异有统计学意义(P<0.05)。结论成功建立类似PRKAG2心脏综合征钠离子通道的HEK-293T细胞模型,为后续研究奠定了基础。     

Tamarind seed coat extract restores reactive oxygen species through attenuation of glutathione level and antioxidant enzyme expression in human skin fibroblasts in response to oxidative stress

ObjectiveTo investigate the role and mechanism of tamarind seed coat extract (TSCE) on normal human skin fibroblast CCD-1064Sk cells under normal and oxidative stress conditions induced by hydrogen peroxide (H₂O₂).MethodsTamarind seed coats were extracted with boiling water and then partitioned with ethyl acetate before the cell analysis. Effect of TSCE on intracellular reactive oxygen species (ROS), glutathione (GSH) level, antioxidant enzymes such as superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase activity including antioxidant protein expression was investigated.ResultsTSCE significantly attenuated intracellular ROS in the absence and presence of H₂O₂ by increasing GSH level. In the absence of H₂O₂, TSCE significantly enhanced SOD and catalase activity but did not affected on GPx. Meanwhile, TSCE significantly increased the protein expression of SOD and GPx in H₂O₂-treated cells.ConclusionsTSCE exhibited antioxidant activities by scavenging ROS, attenuating GSH level that could protect human skin fibroblast cells from oxidative stress. Our results highlight the antioxidant mechanism of tamarind seed coat through an antioxidant enzyme system, the extract potentially benefits for health food and cosmeceutical application of tamarind seed coat.     

蓝莓花青素对由H2O2诱导人脐静脉内皮细胞氧化应激损伤的保护作用

目的 通过建立人脐静脉内皮细胞株（human umbilical vein endothelial cells,HUVECs）氧化应激损伤细胞模型,探讨蓝莓花青素是否能够对H₂O₂引起的细胞损伤具有保护作用。方法 采用过氧化氢（H₂O₂）创建ECV304氧化应激损伤细胞模型,实验分为正常对照组、H₂O₂组、蓝莓花青素低、中、高剂药物组,药物组中加入蓝莓花青素培养2h后,再加入H₂O₂继续培养6h,采用MTT法检测细胞活力,流式细胞术检测细胞周期变化情况及细胞凋亡率。结果 细胞活力与H₂O₂浓度及其作用的时间呈相关性下降趋势,在一定浓度范围内蓝莓花青素对细胞具有保护作用。结论 蓝莓花青素对由H₂O₂引起的氧化应激损伤具有一定保护作用。     

高良姜素对PC12细胞氧化损伤的保护作用及其机制研究

目的:探讨高良姜素对H_2O_2诱导的PC12细胞氧化损伤的保护作用及其作用机制。方法:用H_2O_2损伤PC12细胞建立氧化应激损伤模型,细胞分为正常对照组、模型对照组、高良姜素(低、中、高浓度)组,并给予相应干预措施。采用MTT法检测细胞生长抑制率,采用Annexin-V-FITC凋亡检测试剂盒检测细胞凋亡情况,免疫印迹法检测蛋白表达情况。结果:高良姜素预处理可以减少H_2O_2诱导的PC12细胞氧化应激损伤;高良姜素能明显抑制细胞凋亡,且呈剂量依赖性;高良姜素可以增加PI3K/Akt和Bcl-2家族蛋白比例。结论:高良姜素是通过激活PI3K/Akt信号通路发挥对H_2O_2诱导的PC12细胞氧化损伤的保护作用。     

Phytochemical investigation and in vitro antioxidant activity of an indigenous medicinal plant Alpinia nigra B.L. Burtt

Objective

To investigate antioxidant potential of methanol extract of Alpinia nigra leaves.

Methods

The study was done by using various in vitro methods such as 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), nitric oxide and hydrogen peroxide radical scavenging assays. Phytochemical constituents, total phenolic content and total flavonoid content of the extract at different concentrations (10-500 µg/mL) were determined.

Results

Alpinia nigra leaves showed high free radical scavenging activity as evidenced by the low IC₅₀ values in DPPH (64.51 µg/mL), in ABTS (28.32 µg/mL), in nitric oxide (80.02 µg/mL) and in H₂O₂ (77.45 µg/mL) scavenging assays. Furthermore the TPC and TFC of the extract were found to be 69.25 mg gallic acid equivalent per gram of extract and 78.84 mg quercetin equivalent per gram of extract respectively.

Conclusions

The results of present comprehensive analysis demonstrated that Alpinia nigra leaves possess high phenolic, flavonoid contents and potential antioxidant activity, and could be used as a viable source of natural antioxidants and might be exploited for functional foods and neutraceutical applications.     

Protective effects of Yindanxinnaotong capsule in a rat model of myocardial ischemia/reperfusion injury

ObjectiveTo investigate the effects of Yindanxinnaotong capsule (YDXNTC) and main components compatibility and ratios on myocardium against ischemia/reperfusion injury and the effect's underlying mechanism.MethodsMyocardial ischemia/reperfusion injury (MIRI) was induced by ischemia for 30 min and reperfusion for 30 min. Electrocardiogram data and coronary flow were recorded, and superoxide dismutase (SOD), malondialdehyde (MDA), lactate dehydrogenase, creatine kinase-MB, cardiac troponin T and I (cTnT, cTnI) and interleukin-1β, interleukin-8, interleukin-18 (IL-1β, IL-8, IL-18) in myocardium were measured. Hypoxia/reoxygenation and hydrogen peroxide (H₂O₂) injury were induced by hypoxia for 3 h/reoxygenation for 2 h, and 100 μM H₂O₂ for 1 h, respectively, in vitro rat myocardial cells (H9c2). Cell viability, SOD, MDA, cTnT and inflammatory factors (IL-1β, IL-8 and IL-18) were determined, and Toll-like receptor 4 (TLR-4) expression was measured by western blotting.ResultsIn the isolated heart experiment, elevated heart function, coronary flow and SOD levels, and decreased MDA levels and inflammatory factors were noted in the YDXNTC, main components and main components compatibility groups. Ventricular tachycardia/ventricular fibrillation occurrence decreased in the ginkgo biloba extract (GBE), and GBE and salvia miltiorrhiza ethanol extract compatibility (SM-E, GSEC) groups. Lactic dehydrogenase levels decreased in the YDXNTC and aqueous extract of salvia miltiorrhiza (SM-H) groups. Creatine kinase-MB decreased with GBE, SM-E, SM-H and GSEC treatment, and cTnI and cTnT levels decreased with GSEC. In the in vitro cell study, YDXNTC and main components ratios improved cell viability and SOD levels, and suppressed MDA, cTnT and inflammatory factors. TLR-4 expression was down-regulated.ConclusionYDXNTC and main components compatibility showed protective effects on MIRI in this rat model and in vitro study. Regulating the Toll-like receptor signaling pathway may affect the mechanism.     

Screening of biological activities of Polygonum maritimum L. from Algerian coast

Objective

To investigate the antioxidant and the antibacterial activities of crude extract from aerial part of Polygonum maritimum L. (Polygonaceae) (P. maritimum) and to find new actives biomolecules.

Methods

The whole plant was collected from the Rechgoune coast (West of Algeria), and methanolic crude extract of aerial parts of P. maritimum (PMCE) was prepared. The extract was tested against different bacterial strain and tested for his ability to neutralize free radical (DPPH) and to scavenge the H₂O₂.

Results

PMCE had a very high content of total phenol, which was (352.49±18.03) mg/g dry weight, expressed as gallic acid equivalent. PMCE exhibited excellent antioxidant activity, as measured using DPPH and H₂O₂ scavenging assays. It also showed a high antibacterial activity against gram-positive bacterial strains: Bacillus cereus, Bacillus subtilis and Staphylococcus aureus with an highest MIC of 120 µg/mL.

Conclusions

The antioxidant and antibacterial activity of the PMCE is probably due to phenolic compounds present in the extract. The contemporary presence of antioxidant and antibacterial activities in the PMCE suggests that this plant may be a source of bioactive substances with multifaceted activity.     

胞红蛋白对巨噬细胞氧化损伤的保护作用

目的 探讨CYGB在巨噬细胞氧化损伤过程中的表达及作用。方法构建CYGB高表达/低表达巨噬细胞模型,Western blot检测不同浓度H₂O₂刺激RAW264.7 12 h后CYGB的蛋白表达水平;用分光光度计观察RAW264.7在氧化损伤过程中乳酸脱氢酶(LDH)活性和丙二醛(MDA)含量的变化情况。结果0.5 mmol/L H₂O₂处理巨噬细胞12 h后CYGB表达水平明显高于空白对照组(P<0.05);与0.5 mmol/L H₂O₂处理组相比,0.5 mmol/L H₂O₂对CYGB高表达组的CYGB表达升高(P<0.05),而0.5 mmol/L H₂O₂对CYGB低表达组的CYGB表达降低,差异有统计学意义(P<0.05);0.5 mmol/L H₂O₂₊CYGB高表达组中LDH活性和MDA含量下降;0.5 mmol/L H₂O₂₊CYGB低表达组中LDH活性和MDA含量升高,差异有统计学意义(P<0.05)。结论巨噬细胞氧化损伤时CYGB表达水平增加;CYGB在巨噬细胞氧化损伤过程中具有拮抗作用。     

A simple method for purification of genomic DNA from whole blood using Fe3O4/Au composite particles as a carrier

Objective： To establish a method of genomic DNA extraction from whole blood using Fe3O4/Au composite particles as a carrier. Methods： Two crucial conditions （sodium chloride concentration and amount of the magnetic particles） were optimized and 8 different human whole blood samples were used to purify genomic DNA under the optimal condition. Then agarose gel electrophoresis and polymerase cbain reaction （PCR） were performed. Results： The optimal binding condition was 1.5 mol/L NaC1/10% PEG, and the optimal amount of Fe3O4/Au composite particles was 600μg. The yields of the genomic DNA from 100μl of different whole blood samples were 2-5 μg, and the ratio of A260/A280 was in the range of 1.70-1.90. The size of genomic DNA was about 23 kb and the PCR was valid. Conclusion： The purification system using Fe3O4/Au composite microparticles has advantages in high yield, high purity, ease of operating, time saving and avoiding centrifugation. The purified sample was found to function satisfactorily in PCR amplification.     

Fenton法处理DMF废水及无机阴离子对反应的影响

N,N-二甲基甲酰胺（DMF）作为一种万能溶剂在工业中被广泛应用,但它有毒性、难以生物降解。本文采用Fenton法处理DMF模拟废水,考察了pH、反应时间、氧化剂投加量等条件对处理效果的影响,同时探讨了Cl^-和SO₄^2-对反应的影响。研究结果表明：处理1 g/L的DMF模拟废水,最佳反应条件为：w=30% H₂O₂的投加量为40 mL/L,pH=2.0,n（H₂O₂）：n（Fe²⁺）=3：1,反应时间2.5 h,在该条件下出水化学需氧量（COD）小于100 mg/L;Cl^-对Fenton法处理DMF废水有着十分强的抑制作用,1 g/L就会使出水COD由90 mg/L上升到250 mg/L;而SO₄^2-对Fenton反应的处理效果影响很小,当ρ_SO4^2->10 g/L时有一定的促进作用。     

甜杏仁对H2O2诱导大鼠外周淋巴细胞DNA损伤的影响

目的观察甜杏仁对过氧化氢(H2O2)诱导淋巴细胞DNA损伤的影响。方法提取大鼠淋巴细胞并进行分组。除空白、阳性对照组外,实验组分别加入不同浓度的甜杏仁匀浆液(终浓度分别为50、100、150mg/L);在CO2孵育箱继续温孵24h,当细胞在培养瓶中生长近饱和时,除空白对照组外,其余各组均加入H2O2(终浓度为50μmol/L)作用5min。用单细胞凝胶电泳(SCGE)技术观察各组淋巴细胞DNA损伤情况。结果与空白对照组比较,阳性对照组,加入高、中、低剂量甜杏仁组细胞DNA损伤的程度明显增强(P<0.05);与阳性对照组比较,加入高、中、低不同剂量甜杏仁匀浆液后,细胞DNA的损伤程度明显降低(P<0.05);与低剂量甜杏仁匀浆液组比较,中、高剂量甜杏仁匀浆液组细胞DNA的损伤程度明显减轻,差异均有统计学意义(P<0.05),其中高剂量甜杏仁匀浆液对细胞DNA损伤的保护作用优于中剂量组,但2组间差异无统计学意义(P>0.05)。结论甜杏仁具有一定的抗氧化作用。     

外阴阴道假丝酵母菌病患者阴道乳杆菌的功能变化

目的 分离、纯化、鉴定外阴阴道假丝酵母菌病(VVC)患者阴道乳杆菌菌种;绘制VVC患者阴道内分离的卷曲乳杆菌、加氏乳杆菌、詹氏乳杆菌、发酵乳杆菌的生长曲线,检测其产酸能力、产H₂O₂能力,分析VVC患者阴道乳杆菌的功能变化及临床意义,为VVC的病因及治疗提供理论基础。方法 体外培养VVC患者阴道分泌物中的乳杆菌,分离、纯化,并用分子生物学技术鉴定;测定24h菌液浊度法绘制VVC患者及健康女性阴道内分离的4种乳杆菌的生长曲线;检测24h菌液pH值对比VVC患者与健康女性阴道内分离的卷曲乳杆菌、加氏乳杆菌、詹氏乳杆菌、发酵乳杆菌的产酸能力;绘制H₂O₂浓度标准曲线,定量检测摇菌3h菌液的产H₂O₂的量,对比VVC患者与健康女性阴道内分离的卷曲乳杆菌、加氏乳杆菌、詹氏乳杆菌、发酵乳杆菌的产H₂O₂能力。结果 共分离培养了34株VVC患者阴道乳杆菌,经鉴定,分属于乳杆菌的4个种。VVC患者与健康妇女相比,阴道内卷曲乳杆菌、加氏乳杆菌、詹氏乳杆菌、发酵乳杆菌的增殖速度差异无统计学意义(P<0.05)。健康女性加氏乳杆菌与卷曲乳杆菌的产酸能力比VVC患者强(P<0.05);詹氏乳杆菌的产酸能力两组差异无统计学意义(P>0.05);而VVC患者发酵乳杆菌的产酸能力比健康女性强(P<0.05)。VVC患者阴道内分离的卷曲乳杆菌、加氏乳杆菌、詹氏乳杆菌、发酵乳杆菌的产H₂O₂量均明显少于健康妇女(P<0.05)。结论 本研究为进一步构建中国女性阴道来源的乳杆菌菌种库、进行阴道来源的乳杆菌的相关研究提供菌种。VVC状态下,念珠菌的增多,阴道微环境的改变,并未影响阴道内定植的乳杆菌的生长速度。阴道内乳杆菌的产酸功能并不是抑制VVC的关键。阴道内乳杆菌产H₂O₂能力可能为抑制VVC的关键。