白癜风患者外周血CD4~+CD25~+Foxp3~+和CD4~+CD25~+HLA-DR~+T细胞的测定

为了进一步研究调节性T细胞(Treg)在白癜风发病中的作用,我们对不同病期白癜风患者外周血CD4~+ T细胞中同时表达CD25和在Treg的发生与功能中处中心地位的义头转录因子p3(Foxp3)~([1-2])以及Treg的相天表型分子(HLA-DR)的百分比进行检测.     

CD4+CD25+Treg与特应性皮炎

特应性皮炎(atopic dermatitis,AD)是一种与遗传过敏体质有关的特发性皮肤炎症性疾病,其病因尚不清楚.Th1/Th2平衡失调在AD的发病机制中起重要作用,而CD4+CD25+Treg在Th平衡的打破中可能发挥重要作用.     

寻常型银屑病患者外周血CD4~+CD25~+及CD8~+CD25~+细胞的检测

目的研究进展期寻常型银屑病患者外周血CD4+CD25+和CD8+CD25+调节性T细胞的数量变化及其在银屑病免疫病理学发病机制中的作用。方法应用流式细胞术对进展期寻常型银屑病患者外周血CD4+CD25+和CD8+CD25+调节性T细胞进行检测。结果进展期寻常型银屑病外周血CD4+CD25+细胞及CD8+CD25+调节性T细胞数量与正常对照组相比,均显著降低(P<0.05,P<0.005),而CD4+CD25+/CD8+CD25+比值无显著性差异(P>0.05)。结论寻常型银屑病的发病与CD4+CD25+和CD8+CD25+调节性T细胞的同步降低有关,与二者的比值无关。     

脓毒症患者CD14+CD16+单核细胞的作用探讨

目的观察脓毒症患者外周血单核细胞CD14+CD16-、CD14+CD16+、CD14-CD16+三个亚群表达HLA-DR、CD14、CD16的异同,并分析CD14+CD16+单核细胞的特点。方法选取67例脓毒症患者为脓毒症组和15例健康志愿者作为对照组,脓毒症组根据疾病严重程度分为脓毒症（15例）、重症脓毒症（41例）和脓毒性休克组（11例）,收集临床资料,计算APACHE II评分,流式细胞仪检测单核细胞各亚群CD14、CD16、HLA-DR的表达。结果脓毒症患者CD14+CD16+单核细胞数目较正常对照组显著升高（P＜0.01）;脓毒症患者单核细胞HLA-DR、CD14平均荧光强度均较健康对照组明显降低,并随着脓毒症患者病情由轻到重而逐渐下降;各组中CD14+CD16+单核细胞相对高表达HLA-DR、CD14;单核细胞HLA-DR表达与临床指标间存在较好的相关关系。结论①脓毒症患者单核细胞HLA-DR表达显著降低;②CD14+CD16+单核细胞在脓毒症发病的免疫机制中可能起到重要作用。     

银屑病外周血CD4+CD25+Foxp3+调节性T细胞的表达

目的: 检测CD4+CD25+Foxp3+调节性T细胞在不同类型银屑病患者中的表达.方法: 应用流式细胞仪检测外周血中CD4+CD25+Foxp3+调节性T细胞的表达.结果: 红皮病型银屑病患者外周血中CD4+CD25+Foxp3+调节性T细胞明显高于其他类型银屑病和正常对照组(P<0.05);斑块状银屑病CD4+CD25+Foxp3+T细胞比例高于点滴状患者(P<0.05);脓疱型银屑病患者中脓疱存在患者调节性T细胞比例明显低于脓疱消退患者(P<0.05).结论: CD4+CD25+Foxp3+调节性T细胞通过抑制效应T细胞在银屑病的病情活动中可能发挥重要作用.     

CD4+CD25+调节性T细胞与斑秃的关系

CD4+CD25调节性T细胞是T细胞中具有免疫调节功能的主要细胞群,在调节免疫应答和维持外周免疫耐受中起重要作用.CD4+CD25调节性T细胞的数量减少或功能缺失可能导致自身免疫病的发生.斑秃是一种T细胞介导的累及毛囊的器官特异性自身免疫性疾病.斑秃的动物实验和临床研究均发现,斑秃时存在CD4+CD25+调节性T细胞异常,提示CD4+CD25+调节性T细胞可能参与了斑秃的自身免疫发病机制.     

扁平苔藓患者外周血CD4+CD25+Treg细胞的检测

目的:检测扁平苔藓患者外周血CD4+CD25+调节性T细胞(regulatory T cells,Treg)的数量及FOX3 mRNA的表达.方法:采用流式细胞术对25例扁平苔藓患者和25例健康对照者外周血CD4+CD25+Treg细胞进行检测,并通过RT-PCR方法检测FOXP3转录因子在外周血单个核细胞的表达.结果:泛发性扁平苔藓和口腔扁平苔藓患者外周血CD4+CD25+ Treg细胞数分别为(2.68±2.21)%和(2.38±2.67)%,均低于健康对照者(7.87±1.77)%;扁平苔藓患者的FOPX3表达水平也低于健康对照(P<0.05).结论:CD4+CD25+ Treg细胞数量和功能下降,可能通过导致扁平苔藓患者免疫功能紊乱参与了扁平苔藓的发病.     

活动性皮肌炎CD4+CD25+CD127lo/-调节性T细胞的检测

皮肌炎(DM)是一种自身免疫性结缔组织病.CD4+CD25+调节性T细胞(Treg)是存在于人胸腺和外周血中的一类具有免疫抑制作用的T细胞亚群,该细胞数量减少或者功能异常均可导致自身免疫性疾病的发生.转录因子Foxp3是Treg细胞的特征性检测分子.研究发现,表面标志CD127(IL-7αR)的表达与Foxp3呈负相关,可以代替Foxp3作为Treg细胞的检测标志[1].为研究Treg细胞在活动性DM患者发病机制中的作用,我们分别对17例活动性DM患者和正常人对照外周血中的CD4+CD25+、CD4+CD25+CD127lo/-T细胞的百分比进行检测,同时与患者血清肌酸激酶(CK)含量进行相关性分析.并对其中7例住院患者给予糖皮质激素联合免疫抑制剂治疗,观察治疗后10 d,20 d CD4+CD25+和CD4+CD25+CD127lo/-T细胞百分比的变化.     

白癜风患者外周血CD4+CD25+调节性T细胞的检测

Objective To determine the level of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo at different stages and to study its relationship with the development of vitiligo. Methods Blood samples were collected from 34 outpatients with vitiligo, including 19 cases of progressive vitiligo and 15 cases of stable vitiligo, as well as from 20 normal human controls. Flow cytometry was used to detect the levels of peripheral CD4+ and CD4+CD25+ T lymphocytes in these samples. Results Compared with the controls, the percentage of CD4+CD25+ regulatory T lymphoeytes in peripheral lymphocytes was significantly lower in patients with progressive vitiligo than those in patients with stable vitiligo and normal human con-trois [(2.43±0.30)% vs (3.49±0.39)% and (3.34±0.24)%, both P <0.05], but no significant difference was found between patients with stable vitiligo and normal human controls (P＞0.05). A significantly nega-tive correlation was observed between the percentage of CD4+CD25+ regulatory T lymphocytes and lesion area in patients with progressive vitiligo (r = -0.48, P <0.05), but not in patients with stable vitiligo (P ＞0.05). There was no significant correlation between the course of disease and the percentage of peripheral CD4+CD25+ regulatory T lymphocytes in patients with progressive vitiligo or stable vitiligo (both P ＞ 0.05). Conclusion There is an abnormal proportion of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo, which may be related to the development of vitiligo.     

CD4+ CD25+调节性T细胞及其在性传播疾病中的作用

CD4 CD25 调节性T细胞(CD4 CD25 Treg)是近年来确定的一类具有免疫抑制功能的T细胞亚群,可通过与细胞直接接触和分泌细胞因子如IL-10、TGF-β发挥调节作用,在维持机体免疫自稳、防止自身免疫以及抗感染免疫、肿瘤免疫、移植免疫、变态反应等方面起着重要作用.本文就CD4 CD25 Treg分化发育、表型、作用机制及其在性传播疾病中的作用作一综述.     

白癜风患者外周血CD4+CD25+调节性T细胞的检测

Objective To determine the level of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo at different stages and to study its relationship with the development of vitiligo. Methods Blood samples were collected from 34 outpatients with vitiligo, including 19 cases of progressive vitiligo and 15 cases of stable vitiligo, as well as from 20 normal human controls. Flow cytometry was used to detect the levels of peripheral CD4+ and CD4+CD25+ T lymphocytes in these samples. Results Compared with the controls, the percentage of CD4+CD25+ regulatory T lymphoeytes in peripheral lymphocytes was significantly lower in patients with progressive vitiligo than those in patients with stable vitiligo and normal human con-trois [(2.43±0.30)% vs (3.49±0.39)% and (3.34±0.24)%, both P <0.05], but no significant difference was found between patients with stable vitiligo and normal human controls (P＞0.05). A significantly nega-tive correlation was observed between the percentage of CD4+CD25+ regulatory T lymphocytes and lesion area in patients with progressive vitiligo (r = -0.48, P <0.05), but not in patients with stable vitiligo (P ＞0.05). There was no significant correlation between the course of disease and the percentage of peripheral CD4+CD25+ regulatory T lymphocytes in patients with progressive vitiligo or stable vitiligo (both P ＞ 0.05). Conclusion There is an abnormal proportion of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo, which may be related to the development of vitiligo.     

CD4+CD25+Treg细胞在慢性荨麻疹发病中的作用

CD4+CD25+Treg细胞的功能紊乱在变态反应性疾病发生发展中的作用日益引起人们的关注.新近研究表明慢性荨麻疹是由于Treg细胞数量和(或)功能上的异常所引发.文章阐述了Treg细胞的来源、分类、作用机制及其与慢性荨麻疹之间的关系.     

CD+4 CD+25调节性T细胞在自身免疫病中的意义

阐明CD4 CD2 5调节性T细胞作用机制及其自身免疫病的关系。     

白癜风患者外周血CD4+CD25+调节性T细胞的检测

Objective To determine the level of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo at different stages and to study its relationship with the development of vitiligo. Methods Blood samples were collected from 34 outpatients with vitiligo, including 19 cases of progressive vitiligo and 15 cases of stable vitiligo, as well as from 20 normal human controls. Flow cytometry was used to detect the levels of peripheral CD4+ and CD4+CD25+ T lymphocytes in these samples. Results Compared with the controls, the percentage of CD4+CD25+ regulatory T lymphoeytes in peripheral lymphocytes was significantly lower in patients with progressive vitiligo than those in patients with stable vitiligo and normal human con-trois [(2.43±0.30)% vs (3.49±0.39)% and (3.34±0.24)%, both P <0.05], but no significant difference was found between patients with stable vitiligo and normal human controls (P＞0.05). A significantly nega-tive correlation was observed between the percentage of CD4+CD25+ regulatory T lymphocytes and lesion area in patients with progressive vitiligo (r = -0.48, P <0.05), but not in patients with stable vitiligo (P ＞0.05). There was no significant correlation between the course of disease and the percentage of peripheral CD4+CD25+ regulatory T lymphocytes in patients with progressive vitiligo or stable vitiligo (both P ＞ 0.05). Conclusion There is an abnormal proportion of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo, which may be related to the development of vitiligo.     

白癜风患者外周血CD4+CD25+调节性T细胞的检测

目的 检测不同病期白癜风患者外周血CD4+CD25+调节性T细胞水平,探讨其与白癜风发病的关系.方法 白癜风患者34例,进展期19例,稳定期15例.通过流式细胞仪对不同病期白癜风患者外周血CD4+、CD4+CD25+T细胞水平进行检测,并与20例正常人比较.结果 进展期患者外周血中CD4+CD25+调节性T细胞占外周血淋巴细胞的表达率低于正常对照组(P<0.05);稳定期患者与正常对照组比,差异无统计学意义(P＞0.05);进展期患者低于稳定期患者,差异有统计学意义(P<0.05).进展期患者CD4+CD25+调节性T细胞占外周血淋巴细胞表达率与皮损面积呈负相关(P<0.05),稳定期则无相关性(P＞0.05).进展期与稳定期患者CD4+CD25+调节性T细胞占外周血淋巴细胞水平与病程均无明显相关性(P＞0.05).结论 白癜风患者外周血中存在异常比例的cD4+CD25+调节性T细胞,可能与白癜风的发病有关.     

白癜风患者外周血CD4+CD25+调节性T细胞的检测

Objective To determine the level of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo at different stages and to study its relationship with the development of vitiligo. Methods Blood samples were collected from 34 outpatients with vitiligo, including 19 cases of progressive vitiligo and 15 cases of stable vitiligo, as well as from 20 normal human controls. Flow cytometry was used to detect the levels of peripheral CD4+ and CD4+CD25+ T lymphocytes in these samples. Results Compared with the controls, the percentage of CD4+CD25+ regulatory T lymphoeytes in peripheral lymphocytes was significantly lower in patients with progressive vitiligo than those in patients with stable vitiligo and normal human con-trois [(2.43±0.30)% vs (3.49±0.39)% and (3.34±0.24)%, both P <0.05], but no significant difference was found between patients with stable vitiligo and normal human controls (P＞0.05). A significantly nega-tive correlation was observed between the percentage of CD4+CD25+ regulatory T lymphocytes and lesion area in patients with progressive vitiligo (r = -0.48, P <0.05), but not in patients with stable vitiligo (P ＞0.05). There was no significant correlation between the course of disease and the percentage of peripheral CD4+CD25+ regulatory T lymphocytes in patients with progressive vitiligo or stable vitiligo (both P ＞ 0.05). Conclusion There is an abnormal proportion of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo, which may be related to the development of vitiligo.     

白癜风患者外周血CD4+CD25+调节性T细胞的检测

Objective To determine the level of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo at different stages and to study its relationship with the development of vitiligo. Methods Blood samples were collected from 34 outpatients with vitiligo, including 19 cases of progressive vitiligo and 15 cases of stable vitiligo, as well as from 20 normal human controls. Flow cytometry was used to detect the levels of peripheral CD4+ and CD4+CD25+ T lymphocytes in these samples. Results Compared with the controls, the percentage of CD4+CD25+ regulatory T lymphoeytes in peripheral lymphocytes was significantly lower in patients with progressive vitiligo than those in patients with stable vitiligo and normal human con-trois [(2.43±0.30)% vs (3.49±0.39)% and (3.34±0.24)%, both P <0.05], but no significant difference was found between patients with stable vitiligo and normal human controls (P＞0.05). A significantly nega-tive correlation was observed between the percentage of CD4+CD25+ regulatory T lymphocytes and lesion area in patients with progressive vitiligo (r = -0.48, P <0.05), but not in patients with stable vitiligo (P ＞0.05). There was no significant correlation between the course of disease and the percentage of peripheral CD4+CD25+ regulatory T lymphocytes in patients with progressive vitiligo or stable vitiligo (both P ＞ 0.05). Conclusion There is an abnormal proportion of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo, which may be related to the development of vitiligo.     

白癜风患者外周血CD4+CD25+调节性T细胞的检测

Objective To determine the level of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo at different stages and to study its relationship with the development of vitiligo. Methods Blood samples were collected from 34 outpatients with vitiligo, including 19 cases of progressive vitiligo and 15 cases of stable vitiligo, as well as from 20 normal human controls. Flow cytometry was used to detect the levels of peripheral CD4+ and CD4+CD25+ T lymphocytes in these samples. Results Compared with the controls, the percentage of CD4+CD25+ regulatory T lymphoeytes in peripheral lymphocytes was significantly lower in patients with progressive vitiligo than those in patients with stable vitiligo and normal human con-trois [(2.43±0.30)% vs (3.49±0.39)% and (3.34±0.24)%, both P <0.05], but no significant difference was found between patients with stable vitiligo and normal human controls (P＞0.05). A significantly nega-tive correlation was observed between the percentage of CD4+CD25+ regulatory T lymphocytes and lesion area in patients with progressive vitiligo (r = -0.48, P <0.05), but not in patients with stable vitiligo (P ＞0.05). There was no significant correlation between the course of disease and the percentage of peripheral CD4+CD25+ regulatory T lymphocytes in patients with progressive vitiligo or stable vitiligo (both P ＞ 0.05). Conclusion There is an abnormal proportion of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo, which may be related to the development of vitiligo.     

白癜风患者外周血CD4+CD25+调节性T细胞的检测

Objective To determine the level of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo at different stages and to study its relationship with the development of vitiligo. Methods Blood samples were collected from 34 outpatients with vitiligo, including 19 cases of progressive vitiligo and 15 cases of stable vitiligo, as well as from 20 normal human controls. Flow cytometry was used to detect the levels of peripheral CD4+ and CD4+CD25+ T lymphocytes in these samples. Results Compared with the controls, the percentage of CD4+CD25+ regulatory T lymphoeytes in peripheral lymphocytes was significantly lower in patients with progressive vitiligo than those in patients with stable vitiligo and normal human con-trois [(2.43±0.30)% vs (3.49±0.39)% and (3.34±0.24)%, both P <0.05], but no significant difference was found between patients with stable vitiligo and normal human controls (P＞0.05). A significantly nega-tive correlation was observed between the percentage of CD4+CD25+ regulatory T lymphocytes and lesion area in patients with progressive vitiligo (r = -0.48, P <0.05), but not in patients with stable vitiligo (P ＞0.05). There was no significant correlation between the course of disease and the percentage of peripheral CD4+CD25+ regulatory T lymphocytes in patients with progressive vitiligo or stable vitiligo (both P ＞ 0.05). Conclusion There is an abnormal proportion of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo, which may be related to the development of vitiligo.     

白癜风患者外周血CD4+CD25+调节性T细胞的检测

Objective To determine the level of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo at different stages and to study its relationship with the development of vitiligo. Methods Blood samples were collected from 34 outpatients with vitiligo, including 19 cases of progressive vitiligo and 15 cases of stable vitiligo, as well as from 20 normal human controls. Flow cytometry was used to detect the levels of peripheral CD4+ and CD4+CD25+ T lymphocytes in these samples. Results Compared with the controls, the percentage of CD4+CD25+ regulatory T lymphoeytes in peripheral lymphocytes was significantly lower in patients with progressive vitiligo than those in patients with stable vitiligo and normal human con-trois [(2.43±0.30)% vs (3.49±0.39)% and (3.34±0.24)%, both P <0.05], but no significant difference was found between patients with stable vitiligo and normal human controls (P＞0.05). A significantly nega-tive correlation was observed between the percentage of CD4+CD25+ regulatory T lymphocytes and lesion area in patients with progressive vitiligo (r = -0.48, P <0.05), but not in patients with stable vitiligo (P ＞0.05). There was no significant correlation between the course of disease and the percentage of peripheral CD4+CD25+ regulatory T lymphocytes in patients with progressive vitiligo or stable vitiligo (both P ＞ 0.05). Conclusion There is an abnormal proportion of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo, which may be related to the development of vitiligo.