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不饱和脂酸及其硒化物对BEL—7402人肝癌细胞的杀伤作用及DNA合?… 总被引:1,自引:0,他引:1
研究了亚麻酸、亚油酸、油酸及其硒化物对人肝癌细胞杀伤作用及对DNA合成的影响。结果表明,三种脂酸及其硒化物对BEL-7402人肝癌细胞均有杀伤作用,其中以硒化亚麻酸杀伤作用最强,500μg/ml即可杀灭全部肝癌细胞,且具剂量效应关系,其次是亚麻酸、亚油酸和硒化亚油酸。油酸和硒化油酸杀伤作用较弱。150μg/ml的亚麻酸,亚油酸及其硒化物和硒化油酸对肝癌细胞的DNA合成均有明显抑制作用(P〈0.01 相似文献
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三种脂肪酸对人肝癌细胞DNA合成和甲胎蛋白分泌的影响 总被引:5,自引:0,他引:5
谢丽涛 《癌变.畸变.突变》1998,10(5):298-300
本实验目的探讨具有相同碳原子长度的亚麻酸,亚油酸及油酸处理的BEL-7402人肝癌细胞后对DNA合成和甲胎蛋白分泌抑制的影响。放射免疫法检测肝癌细胞分泌甲胎蛋白含量,^3H-TdR掺入法测定DNA合成放射活性。实验结果表明,三种脂肪酸能抑制肿癌细胞DNA合成,其中亚麻酸,亚油酸抑制作用明显,有显著性差异,油酸作用较;40-50μg/ml亚麻酸能明显地抑制肝癌细胞AFP分泌,亚油酸和油酸与对照相比无 相似文献
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硒化亚油酸及硒化亚麻酸对肿瘤细胞DNA合成的影响 总被引:1,自引:0,他引:1
本文报道用氚一胸腺嘧啶核苷(^3H-TdR)掺入法研究了新型抗癌药-硒化亚油酸及硒化亚麻酸对小鼠S180细胞DNA合成的影响。结果表明,这两种新型抗肿瘤药物能明显抑制^3H-TdR掺入肿瘤细胞,在100μg/ml的浓度下,它们对S180瘤细胞DNA合成的抑制率分别达到89.0%和88.3%,这些结果表明硒化亚油酸及硒化亚麻酸的抗癌机理之一是它们对^3H-TdR掺入肿瘤细胞的干扰。 相似文献
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目的探讨维拉帕米抑制肝癌耐药细胞增殖的机理。方法同位素掺入法研究细胞的增殖;荧光分光光度法测定细胞内抗癌药物浓度。结果单独应用VPM对耐药肝癌细胞BEL┐7402/ADR有不同程度的抑制作用。而与ADR合用,ADR与对细胞增殖基本无影响的2.5μg/ml浓度的VPM合用时,IC50较单独用ADR时明显降低(P<0.05);而与5.0μg/ml的VPM合用时,其IC40较单独用ADR降低更显著(P<0.01)。VPM可显著增加细胞内的抗癌药浓度(P<0.05~0.01)。结论VPM降低ADR的IC50、增加抗癌药的细胞毒性,可能与其拮抗p┐170糖蛋白有关 相似文献
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肝癌患者全血,癌组织和癌旁组织的硒水平的分析 总被引:2,自引:0,他引:2
1990年5月至1992年5月收集我院经手术切除病理确诊为原发性肝癌患者52例,其中男49例,女3例,年龄22~69岁,中位年龄47.5岁。采用2,3——二氨基苯荧光测定全血、癌组织和癌旁组织与健康人全血硒的含量,结果表明,肝癌患者全血硒的含量(平均值0.l17±0.025μtg/ml)比健康人74例全血硒的含量(平均值0.130±0.023μg/ml)。偏低(P\0.2),而小肝癌15例全血硒的含量(平均值O.123+0.028μg/ml)比浸润型巨大肝癌10例全血硒的含量(平均值0.098±O.023μg/ml)有明显的增加(p<0.05)。无肝硬化的肝癌病人7例全血硒的含量(平均值0.129土0.05μg/ml比中重度肝硬化的肝癌病人16例全血硒的含量(平均值0.109±0.033μg/ml)有明显的增加(P\0.05);肝癌病织硒的含量(平均值O.308±0.114μg/克)比癌旁组织硒的含量(平均值0.474±0.146μg/g)有非常显著的减少(P<0.001),表明肝癌患者的全血、癌组织和癌旁组织硒的含量与临床分期和肝实质损害的程度有相关。 相似文献
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亚硒酸钠和叶绿酸抗致癌物诱发BALB/3T3细胞DNA非程序合成的研究 总被引:2,自引:0,他引:2
本文报道106~104mol/L亚硒酸钠(Na2SeO3)和0.1mg~0.2mg/ml叶绿酸对致癌物N-甲基-N’-硝基-N-亚硝基胍(MNNG)和苯并[a]芘(BaP)诱发BALB/3T3细胞DNA非程序合成(UDS)有明显的抑制作用(P<0.05~0.001)。加致癌物前24小时加入Na2SeO3或叶绿酸,其抑制作用大于同时加入者(P<0.01~0.001)。Na2SeO3与叶绿酸联合作用比单独使用抑制作用更强(P<0.001)。加入超氧化物歧化酶(SOD)抑制剂二乙基二硫代氨基甲酸钠能减弱叶绿酸对两种致癌物的抑制作用(P<0.01和<0.05),提示其作用机理可能与SOD消除自由基有关。 相似文献
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将作者首次合成的硒化蓖麻酸及蓖麻酸分别制成乳剂和多相脂质体,进行各种剂型的动物体内外抗癌活性研究。结果,在体外500μg/ml的浓度下,蓖麻酸乳剂和硒化蓖麻乳剂都能100%地杀伤S180腹水型瘤细胞,而对照组瘤组织有97%存活。在体内蓖麻酸乳剂给小鼠腹腔注射200mg/kg,对S180实体瘤的抑制率为30.6%(P<0.05);相同剂量下硒化蓖麻酸乳剂的抑瘤率为33.3%(P<0.05);蓖麻酸多 相似文献
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应用乳酸脱氢酶释放实验观察人肝癌肿瘤浸润性淋巴细胞(TIL)对经肿瘤坏死因子Alpha(TNF)和干扰素Alpha(IFN)培养过的人肝癌细胞体外抗瘤活性。结果显示经TNF(100U/ml)或IFN(100U/ml)培养过的人肝癌细胞对人肝癌TIL的敏感性明显增强(P<0.05),两者合用,结果更显著(P<0.01);应用MTT法观察TNF和IFN对人肝癌细胞增殖能力的影响,发现TNF对人肝癌细胞的增殖具有抑制作用(P<0.05),而IFN无此现象(P>0.05),两者合用时抑制作用明显(P<0.01)。上述结果表明TNF和IFN可以通过直接或/和间接的方式增加肝癌细胞时其TIL的敏感性,进一步的研究将具有潜在价值。 相似文献
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本文应用人全血白细胞非程序DNA合成(UDS)试验,对硒化亚油酸(Se-LA)和亚硒酸钠(Na2SeO3)两者抑制MNNG诱发DNA损伤作用进行了观察和比较。结果表明:Se-LA(0.023~3.424μg/ml)对MNNG诱发UDS反应有明显抑制作用,其抑制率远大于含硒量相同的Na2SeO3;0.05μg/mlNa2SeO3(含Se量为0.023μg/ml)对UDS反应呈最强的抑制率(21.7%),随Na2SeO3浓度增高,其抑制作用反而降低,说明Na2SeO3作为抗突变剂,它在高剂量时又呈现遗传毒性;当含Se量≥0.046μg/ml时,Na2SeO3对MNNG诱发DNA损伤的抑制作用显著下降,而Se-LA仍保持较高的抑制率(42.2%~43.9%),表明Se-LA明显降低了硒的毒性 相似文献
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钳蝎蝎毒组分Ⅱ对人直肠腺癌C1184细胞生长的影响 总被引:6,自引:0,他引:6
蝎毒组分Ⅱ(ScorpionVenomcrudeⅡ,SVCⅡ)为河南马氏钳蝎粗毒(SVC)经分离纯化后获得的有效成分之一。实验结果表明,以丝裂霉素作为阳性对照,SVCⅡ(4~20μg/ml)对人直肠腺癌C1184细胞株有明显杀伤作用。与空白对照相比,SVCⅡ使细胞生长抑制率、细胞毒性和克隆形成抑制率增大(P<0.05或(P<0.01),并存在剂量效应关系,其半数抑制浓度分别为11.20μg/ml、11.93μg/ml和17.38μg/ml。从生长曲线可见,SVCⅡ对细胞生长呈剂量和时间依赖性抑制。SVCⅡ还能使细胞分裂指数下降。提示SVCⅡ具有抗癌细胞增殖作用。 相似文献
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Bacteria and cancer--antagonisms and benefits 总被引:1,自引:0,他引:1
H C Nauts 《Cancer surveys》1989,8(4):713-723
There is considerable historical and recent evidence concerning the antagonisms between acute bacterial infections or their toxins and cancer and allied diseases. These data provide renewed incentives to undertake clinical programmes with mixed bacterial vaccines in many countries at the present time. 相似文献
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The literature suggests that religiosity helps cope with illness. The present study examined the role of religiosity in functioning among African Americans and Whites with a cancer diagnosis. Patients were recruited from an existing study and mailed a religiosity survey. Participants (N = 269; 36% African American, 56% women) completed the mail survey, and interview data from the larger cohort was utilized in the analysis. Multivariate analyses indicated that in the overall sample religious behaviors were marginally and positively associated with mental health and negatively with depressive symptoms. Among women, religious behaviors were positively associated with mental health and negatively with depressive symptoms. Religiosity was not a predictor of study outcomes for men. Among African Americans, religious behaviors were positively associated with mental health and vitality. Among Whites, religious behaviors were negatively associated with depressive symptoms. These findings suggest a mixed role of religious involvement in cancer outcomes. The current findings may have applied potential in the areas of emotional functioning and depression. 相似文献
16.
J E Moulder J S Holcenberg B A Kamen M Cheng B L Fish 《International journal of radiation oncology, biology, physics》1986,12(8):1415-1418
We used a rat model to study the effects of renal irradiation on the pharmacology of methotrexate (MTX) and cisplatinum (cis-Pt). Unanesthetized rats were given bilateral kidney irradiation (20 Gy in 9 fractions). At 9 months after irradiation, 3% of the animals had died and survivors showed moderately impaired renal function. At 15 months, 30% of the animals had died and survivors showed severely impaired renal function. Some animals were given i.v. MTX 1 week to 15 months after irradiation. In irradiated rats, the area under the MTX plasma clearance curve equaled that of controls through 6 months, and was significantly above controls from 9 months on. Other animals were given i.p. cis-Pt 1 week to 9 months after irradiation. The acute toxicity of cis-Pt was the same in control and irradiated rats when cis-Pt was given immediately before or after irradiation. Beginning 3 months after irradiation there was a progressive increase in cis-Pt toxicity and a simultaneous decrease in urinary platinum excretion. Irradiated animals that survived cis-Pt treatment showed increased radiation nephritis; the greatest effect occurred when cis-Pt was given 3 months or more after irradiation. MTX and cis-Pt clearance decreased when renal dysfunction was first observed and changes in renal function preceded changes in drug clearance and toxicity. 相似文献
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目的:探讨VEGF和KDR在大肠腺瘤和大肠腺癌中的表达及临床病理特征的关系。方法:大肠腺瘤和大肠腺癌组织标本各100例,采用免疫组织化学染色法检测VEGF和KDR在标本中的表达情况。结果:VEGF和KDR在大肠腺癌组中的阳性表达明显高于大肠腺瘤组(P〈0.05);在正常大肠黏膜均未见VEGF和KDR表达的阳性染色;VEGF阳性表达组中KDR的阳性表达率为70%,显著高于VEGF阴性表达组中KDR的阳性表达率16%,两组比较有统计学意义(P〈0.01)。结论:大肠腺癌组织中KDR的表达与肿瘤大小、转移情况、浸润深度密切相关;VEGF和KDR在大肠腺瘤中的表达与患者的年龄、性别及分型均无相关性,而与增生程度相关(P〈0.05)。在大肠腺癌患者中VEGF及KDR表达更高,二者具有协同效应。 相似文献
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Genetic polymorphisms of alcohol and aldehyde dehydrogenases and risk for esophageal and head and neck cancers 总被引:8,自引:0,他引:8
Alcoholic beverages are causally related to cancer of the oral cavity, pharynx, larynx and esophagus. Ethanol is oxidized to acetaldehyde and then to acetate by alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), both of which have genetic polymorphisms. A review of case-control studies of the effects of ALDH2, ADH2 and ADH3 genotypes shows consistently positive associations between inactive heterozygous ALDH2 and the less-active ADH2 genotypes and the risk for esophageal cancer in East Asian heavy drinkers and this enzyme-related vulnerability may extend to light-to-moderate drinkers. Some studies suggest similar associations with the risk for head and neck cancer in moderate-to-heavy-drinking Japanese. An established carcinogen in experimental animals, acetaldehyde can interact with human DNA. ALDH2-associated cancer susceptibility fits into a scenario in which acetaldehyde plays a critical role in the development of human cancer. Alcohol flushing and drinking behavior may partly explain this carcinogenic effect in carriers of less-active ADH2 genotypes. Whether the ADH3 genotype influences head and neck cancer risk in Western nations is controversial. Professional and public education about risky conditions connected to the ALDH2 and ADH2 genotypes and environmental factors is important in a new strategic approach to the prevention of alcohol-related cancers in East Asians. The use of simple tests to identify inactive ALDH2 on the basis of alcohol flushing responses could benefit many people, by helping them to identify their own cancer risks. Such testing could also help clinicians diagnose esophageal cancer earlier, through the use of endoscopic screening in the high-risk population. 相似文献
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Cheryl L. Holt PhD Robert A. Oster PhD Kimberly S. Clay PhD Julie Urmie PhD Mona Fouad MD MPH 《Journal of psychosocial oncology》2013,31(4):372-393
The literature suggests that religiosity helps cope with illness. The present study examined the role of religiosity in functioning among African Americans and Whites with a cancer diagnosis. Patients were recruited from an existing study and mailed a religiosity survey. Participants (N = 269; 36% African American, 56% women) completed the mail survey, and interview data from the larger cohort was utilized in the analysis. Multivariate analyses indicated that in the overall sample religious behaviors were marginally and positively associated with mental health and negatively with depressive symptoms. Among women, religious behaviors were positively associated with mental health and negatively with depressive symptoms. Religiosity was not a predictor of study outcomes for men. Among African Americans, religious behaviors were positively associated with mental health and vitality. Among Whites, religious behaviors were negatively associated with depressive symptoms. These findings suggest a mixed role of religious involvement in cancer outcomes. The current findings may have applied potential in the areas of emotional functioning and depression. 相似文献
20.
Metalloproteinases and cancer invasion and metastasis 总被引:7,自引:0,他引:7
G Murphy J J Reynolds R M Hembry 《International journal of cancer. Journal international du cancer》1989,44(4):757-760