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A link between p73 transcriptional activity and p73 degradation 总被引:3,自引:0,他引:3
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Mdm2 binds p73 alpha without targeting degradation. 总被引:7,自引:0,他引:7
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Inactivation of the p53-homologue p73 by the mdm2-oncoprotein. 总被引:10,自引:0,他引:10
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Hosoda M Ozaki T Miyazaki K Hayashi S Furuya K Watanabe K Nakagawa T Hanamoto T Todo S Nakagawara A 《Oncogene》2005,24(48):7156-7169
p73 protein level is kept extremely low in mammalian cultured cells and its stability may be regulated by not only the ubiquitin/proteasome-dependent proteolysis but also through other unidentified mechanisms. Here, we found for the first time that p73 is physically as well as functionally associated with the U-box-type E3/E4 ubiquitin ligase UFD2a. The immunoprecipitation experiments demonstrated that this interaction is mediated by the COOH-terminal region of p73alpha containing SAM domain. During the cisplatin-induced apoptosis in SH-SY5Y neuroblastoma cells, p73alpha accumulated at a protein level, whereas the endogenous UFD2a was significantly reduced in response to cisplatin. Ectopic expression of UFD2a decreased the half-life of p73alpha in association with a significant inhibition of the p73alpha-mediated transactivation as well as proapoptotic activity. Downregulation of endogenous UFD2a by antisense strategy resulted in a remarkable accumulation of p73alpha. Unexpectedly, UFD2a-mediated degradation of p73alpha was sensitive to the proteasomal inhibitor, however, UFD2a did not affect the ubiquitination levels of p73alpha. Taken together, our present findings imply that UFD2a might promote the proteasomal turnover of p73 in a ubiquitination-independent manner, and also suggest that UFD2a might play an important role in the regulation of cisplatin-induced apoptosis mediated by p73. 相似文献
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Takahiko Kobayashi Junich Ishida Manabu Musashi Shuichi Ota Takeshi Yoshida Yuichi Shimizu Makoto Chuma Hiroshi Kawakami Masahiro Asaka Junji Tanaka Masahiro Imamura Masanobu Kobayashi Hiroshi Itoh Hironori Edamatsu Leslie C. Sutherland Rainer K. Brachmann 《International journal of cancer. Journal international du cancer》2011,128(2):304-318