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1.
The immunocytochemical and ultrastructural features within [Met]enkephalin neurons of the guinea-pig hypothalamoseptal tract were investigated under chronic inhibition of GABAergic catabolism. This was achieved by raising the brain GABA concentration with aminooxyacetic acid which inhibits GABA-transaminase, the enzyme responsible for the catabolism of GABA. Guinea-pigs were injected intraperitoneally with 10 or 20 mg/kg per day aminooxyacetic acid for two, four or eight days and killed 16 h post-dose. Repeated injections of aminooxyacetic acid produced a great increase in immunoreactivity for GABA in nerve endings surrounding enkephalinergic perikarya in the magnocellular dorsal nucleus of the guinea-pig. Extensive immunocytochemical studies stressed the increase and redistribution of the immunoreaction for [Met]enkephalin in the perikarya of the magnocellular dorsal nucleus under such GABAergic activation. Quantitative and statistical analyses showed that administration of aminooxyacetic acid for eight days significantly increased the intensity of labelling within stimulated perikarya (P less than 0.001). A concomitant accumulation of immunopositive large granules in the enkephalinergic boutons of the lateral septum was observed. In the same way, ultrastructural changes in enkephalinergic cell bodies were analysed and reflected disturbances in the biosynthetic and digestive activities of enkephalinergic perikarya. We postulate that chronic inhibition of the GABAergic catabolism leads to modification in the metabolism of enkephalinergic neurons and to an inhibitory action of GABA on the [Met]enkephalin release from nerve endings. This study give morphological support to the complex functional interactions between GABA and opioid peptide transmitter system.  相似文献   

2.
The opioid peptide enkephalin emerges as a major neuromodulator in the regulation and integration of the physiologic response in stressful conditions. The paraventricular nucleus of the hypothalamus is a coordinating center of neuroendocrine and autonomic functions. However, the detailed distribution of the enkephalin fibers and terminals in the paraventricular nucleus and the sources of enkephalinergic innervation are not well defined. In the present study, we used immunocytochemistry for the proenkephalin-derived octapeptide met-arg6-gly7-leu8 enkephalin to determine the distribution of enkephalin-immunoreactive fibers and somata within paraventricular nucleus. Without colchicine pretreatment, enkephalinergic fibers were prominent mainly in the ventromedial part of the parvicellular subdivision of the paraventricular nucleus, appearing in coronal sections as a dense collection of short segments of enkephalin-immunoreactive fibers. In the periventricular portion of the paraventricular nucleus, enkephalin-immunoreactive fibers produced a moderate plexus of short enkephalin-immunoreactive fibers dorsoventrally oriented. With colchicine treatment, a dense cluster of enkephalin-immunoreactive cell bodies was located in the dorsomedial and the dorsal parts of the parvicellular subdivisions. These enkephalin-immunoreactive neurons were small (< 10 μm) to medium sized (10–15 μm), with round and elongated shapes. Retrograde transport of wheat germ-conjugated gold particles. WGA-apoHRP-Au, from the paraventricular nucleus, combined with immunocytochemistry for enkephalin revealed that the major sources of extrahypothalamic enkephalin afferents to the paraventricular nucleus are provided by enkephalin neurons neurons in the lateral reticular nucleus and the paragigantocellularis reticular nucleus of the medulla (20% of retrogradely labeled neurons within this nucleus were double labeled) and in the nucleus solitary tract (10% of retrogradely labeled neurons within thisnucleus were double labeled). Retrogradely labeled enkephalin neurons were also observed in the medial preoptic area, median preoptic nucleus, dorsomedial hypothalamic nucleus, lateral septum and hypothalamic arcuate nucleus. These enkephalinergic pathways from the medulla and the forebrain could represent an anatomical substrate underlying the opioid effects on paraventricular neurons during physiological processes, such as a cardiovascular regulation, feeding or stress responses.  相似文献   

3.
The distribution of neurons expressing preproenkephalin (PPE) mRNA in the brain of the salmon was investigated by means of non-radioactive in situ hybridization, and directly compared with the distribution of enkephalin-immunoreactive (ENKir) neurons. This approach, utilized here for the first time in a non-mammalian vertebrate for the identification of neurons containing opioid peptides, permitted a detailed analysis of the distribution of putative enkephalinergic neurons in the salmon brain. Several cell groups containing neurons that express PPE mRNA also contain ENKir neurons. Such cell groups are located in the ventral telencephalic area, the nucleus of the rostral mesencephalic tegmentum and another nucleus immediately dorsal to it, the torus semicircularis, the valvula cerebelli and the corpus cerebelli. These cell groups consistently contain larger numbers of PPE mRNA expressing cells than ENKir ones. Some cell groups express PPE mRNA, but do not contain ENKir neurons. These cell groups are located in the dorsal telencephalic area, the inferior lobes of the hypothalamus, the pretectal area, the magnocellular superficial pretectal nucleus, the optic tectum, the oculomotor nucleus, the trochlear nucleus, the magnocellular vestibular nucleus, the secondary gustatory nucleus, the superior and medial reticular nuclei, the motor nucleus of the vagus and the ventral horn of the spinal cord. Moreover, some cell groups contain ENKir neurons, but no PPE mRNA expressing neurons. These cell groups are located in the ventromedial thalamic nucleus, the lateral tuberal nucleus, the nucleus of the lateral recess and the nucleus of the posterior recess. The majority of these periventricular ENKir neurons were of the cerebrospinal fluid-contacting type. ENKir neurons were also located in the dorsal lateral tegmental nucleus and in area B9. The results also permitted a tentative identification of enkephalinergic neurons afferent to the optic tectum, that have previously not been identified with immunocytochemistry, located in the dorsal telencephalic area, as well as enkephalinergic neurons intrinsic to the tectum that may contribute to the laminar arrangement of ENKir fibers in the optic tectum.  相似文献   

4.
目的:观察发自前庭神经内侧核的纤维末梢与投射至纹状体的丘脑束旁核神经元的突触联系。方法:采用15只Wistar大鼠,应用顺行和逆行标记技术,免疫组织化学和免疫电镜方法。结果:将CTb单侧注入纹状体,同时将BDA注入同侧的前庭神经内侧核。在束旁核发现了CTb标记神经元和BDA标记轴突终末,BDA标记纤维和终末存在于外侧束旁核整个长度的背侧2/3区,而CTb标记神经元也存在于外侧束旁核背侧2/3区,2种标记相互重叠。电镜下可见标记终末与标记神经元形成非对称性的轴-体和轴-树突触。结论:由前庭神经内侧核发出的投射纤维在束旁核与投射至纹状体的束旁核神经元之间存在着非对称性的突触联系。  相似文献   

5.
Summary Direct projections from Ammon's horn to the septum were studied in the cat by the anterograde tracing method after injecting WGA-HRP (wheat germ agglutinin-horseradish peroxidase conjugate) into Ammon's horn. The results were further confirmed by the retrograde WGA-HRP method after injecting WGA-HRP into the septum. Pyramidal neurons in fields CA1, CA2 and CA3 were observed to send their axons ipsilaterally to the lateral septal nucleus; the septal parts of the hippocampus sent projection fibers to the dorsomedial portions of the lateral septal nucleus via the medial aspects of the subcallosal fornix, while the hippocampal regions successively more proximal to the temporal pole sent projection fibers to progressively more ventrolateral portions of the lateral septal nucleus via more lateral aspects of the subcallosal fornix. It was also found that the septal parts of fields CA1, CA2 and CA3 sent projection fibers bilaterally to the dorsomedial aspects of the lateral septal nucleus. Field CA4 appeared to send projection fibers only sparsely, if at all, to the medial septal nucleus. The rudimentary parts of the hippocampal formation, taenia tecta and indusium griseum, were found to have reciprocal ipsilateral connections with the dorsal portions of the lateral septal nucleus.  相似文献   

6.
Connections of Purkinje cell axons of lobule X (nodulus vermis) with vestibulospinal neurons have been demonstrated in the rat, by anterograde labeling of axons with biotinylated dextran (BD) injected into sublobule Xa and by retrograde labeling of neurons with cholera toxin subunit B (CTB) injected into cervical segments. Labeled terminals of Purkinje cell axons were numerous in the superior vestibular nucleus, the parvocellular (MVpc) and the caudal part (MVc) of the medial vestibular nucleus (MV), and group y. A limited number of labeled terminals were seen in the caudal part of the descending vestibular nucleus (DV). Occasional labeled terminals were seen in the lateral part of the lateral vestibular nucleus (LV) whereas few labeled terminals were seen in the magnocellular part of the MV (MVmc). Vestibulospinal neurons labeled from the C2 and C3 segments were seen bilaterally in the MVmc, MVpc, MVc, and DV, and ipsilaterally in the LV. CTB-labeled vestibulospinal neurons in contact with BD-labeled terminals of Purkinje cell axons were identified in the lateral part of the MVpc, near the border between the MVpc and MVmc, or close to the dorsal acoustic stria, and in the middle part of the MVc at its rostral level. The present study suggests that Purkinje cells of lobule X regulate the output of cervical-projecting vestibulospinal neurons in the MVpc and MVc.  相似文献   

7.
In order to investigate whether chemoarchitecture would support the subdivision of the anuran septum based on cytoarchitectonic and hodological studies, we performed enzyme-histochemical detection of NADPH-diaphorase and immunohistological demonstration of choline-acetyl transferase (ChAT), aspartate, calretinin, gamma-aminobutyric acid (GABA), 5-hydroxy-tryptamine, tyrosine hydroxylase, neuropeptide Y (NPY), somatostatin, Leu- and Leu + Met-enkephalin, and substance P in the fire-bellied toad Bombina orientalis. Labeling of cell bodies matched well the previously defined subnuclei: The dorsolateral septal nucleus contains enkephalin-immunoreactive (-ir) and weakly stained GABA-ir neurons; calretinin-ir and weakly labeled GABA-ir neurons are found in the ventrolateral septal nucleus. The medial septal nucleus is characterized by the presence of numerous ChAT-ir and some tyrosine hydroxylase-ir neurons, while the dorsal septal nucleus is outlined by its NPY-ir neurons. Many ChAT-ir and some aspartate-ir and somatostatin-ir neurons are found in the diagonal band of Broca, and the central septal nucleus contains some GABA-ir and ChAT-ir neurons. In contrast, labeled fibers form a pattern which does not match the boundaries of septal subnuclei. Comparing the anuran septal complex with that of other vertebrates reveals that the complexity of the lateral septum has increased during the evolution from anamniote to amniote vertebrates. In spite of this fact, many similarities in chemoarchitecture between anurans and other vertebrates are evident. Some basal septal functions such as involvement in learning and memory formation or inhibition of sexual behavior appear to have persisted during vertebrate evolution.  相似文献   

8.
Cells of origin of the descending afferents to the lateral hypothalamic area (LHA) were examined following iontophoretic injections of horseradish peroxidase (HRP). HRP-positive neurons were found ipsilaterally and mainly in the following structures: the infralimbic cortex (area 25), septal area including the nucleus of the diagonal band, nucleus accumbens, olfactory tubercle, medial and lateral preoptic areas, preoptic magnocellular nucleus, substantia innominata, bed nucleus of the stria terminalis and the central amygdaloid nucleus. Of these, neurons of the nucleus accumbens, olfactory tubercle, preoptic magnocellular nucleus and substantia innominata were preferentially labeled when the enzyme was deposited in the lateral part of the LHA.  相似文献   

9.
Summary Antibodies to the neuropeptides somatostatin (SOM) and neurotensin were used to study the distribution of the two peptides within the septum of the rat brain. In colchicine treated rats, numerous somatostatin-positive cell bodies were found in the dorsal and ventral subdivisions of the alteral septum, along the border of the nucleus accumbens, in the ventral tip of the horizontal limb of the diagonal band of Broca as well as in the anterior hippocampal rudiment, infralimbic area and several other structures of the basal forebrain (e.g., nucleus accumbens, olfactory tubercle and substantia innominata). Cell bodies containing immunoreactivity for neurotensin were situated in the intermediate and ventral subdivisions of the lateral septum, the medial septal nucleus, the diagonal band of Broca, the rostro-medial continuation of the substantia innominata and the olfactory tubercle.In untreated rats, somatostatin positive processes formed terminal plexuses in the medial septal nucleus and along an area close to the ventricular wall of the lateral ceptal nucleus. Other septal nuclei, such as the diagonal band of Broca contained a sparse innervation by somatostatin positive fibers. In contrast, the nucleus accumbens olfactory tubercle, and the substantia innominata contained a rich innervation by somatostatin positive axons and terminals. Within these structures the density of SOM positive processes show great variations with patches of densely packed terminals separated by areas of sparser or no innervation. The neurotensin positive terminals were situated predominantly within the intermediate part of the lateral septum and the medial septal nucleus. Both of these regions contained numerous pericellular baskets of neurotensin positive terminals around septal neurons. In addition to the septal innervation, several of the basal forebrain structures were rich in neurotensin positive processes with the densest innervation found in the nucleus accumbens and substantia innominata. Like the SOM-immunoreactivity distinct islands of dense neurotensin innervation separated by less or no innervation occur throughout the basal forebrain. Taken together, these findings suggest that somatostatin and neurotensin occur in separate neuronal populations and that each may influence important physiological functions within the individual septal nuclei.  相似文献   

10.
The distribution of gamma aminobutyric acid (GABA)-containing neurons and nerve terminals was examined in the rat septal area by using specific antibodies to the enzyme glutamic acid decarboxylase (GAD) in combination with the avidin-biotin immunoperoxidase method. Whereas only a few GAD positive neurons were present in the septum of normal rats, the septal area of rats treated with colchicine, an inhibitor of fast axonal transport, showed numerous GAD-immunoreactive neurons. These neurons were evenly filled with GAD-immunoreactive material throughout the cytoplasm of the soma and proximal parts of the dendrites. Although GAD-positive neurons were present in most parts of the septal area, their density differed greatly in the different septal subnuclei. Both the diagonal band of Broca (vertical and horizontal parts) and the lateral septum were rich in GAD positive cell bodies, whereas the medial septal nucleus and the intermediate parts of the lateral septum contained relatively few. Within the lateral septum itself a larger number of labeled cell bodies was present in its ventral subdivision. The anterior hippocampal rudiment (taenia tecta) contained numerous GAD-positive neurons, while the septal component of the island of Calleja (insula magna) was devoid of them. GAD-immuno-positive neurons found within the septum ranged from small (15 microns) to large (30-35 micron). They were round or multipolar in the diagonal band, medium-sized multipolar in the lateral septum, and pyramidal, round or fusiform in the anterior hippocampal rudiment. GAD-immunoreactive nerve terminals are present in most subdivisions of the septal nuclei, with the exception of myelinated fiber tracts, and throughout all rostrocaudal levels of the septum. However, the density of the innervation is not the same within all individual nuclei. The lateral septum (dorsal and ventral parts) contained high density innervation but the diagonal band of Broca had a lower density of GAD-positive terminals. The lateral border of the islands of Calleja was rich in thick GAD-positive processes that appeared to be continuous with GAD-immunoreactive processes of the substantia inominata. The inner portion of the molecular layer adjacent to the granule cells of the anterior hippocampal rudiment contained a rich GAD-positive terminal field.  相似文献   

11.
为也观察下丘脑外侧核乙酰胆碱酯酶阳性神经元的传入纤维联系。方法;采用HRP与Ache相结合的双标记方法,对17只大鼠下丘脑外侧核进行了逆行追踪研究。结果;在下列核团观察到HRP-AchE双标记细胞;斜角带核垂直支腹侧部,斜角带核水平支隔内侧核,中缝背核,蓝斑,臂旁核,下丘脑前核,对侧下丘脑外侧核,小脑间位核及小脑齿状核。  相似文献   

12.
Summary The morphology of the retina and central retino-recipient nuclei was studied in two monkeys that had undergone total bilateral striate cortex removal as adults. These animals had been behaviorally tested for two years after lesioning and had demonstrated significant recovery of pattern vision. Light and electron microscopy and autoradiography were done on the central retino-recipient nuclei following a monocular intravitreal injection of 3H-proline.Light microscopic analysis of retinal ganglion cell number showed a 30% loss in the parafoveal retina due to retrograde trans-synaptic degeneration.The most striking central change in retinal axon distribution was in the dorsal lateral geniculate nucleus (dLGN) where the parvocellular but not the magnocellular region showed a marked reduction in retinal input. Despite the loss of almost all dLGN neurons through retrograde degeneration, at the EM level both parvocellular and magnocellular regions contained islands of neuropil made up of retinal and several other types of synaptic terminals as well as small dendrites and pale unidentified processes. Approximately equal numbers of retinal terminals were identified by EM autoradiography in both regions of dLGN, which did not explain the apparent differences in labeling between the two regions seen in the light microscope.A second change in central retinal pathways was found in the olivary pretectal nucleus where a significant loss of retinal input also occurred. A third change, but in the opposite direction, was found in the pregeniculate nucleus (PGN) where the area of retinal terminals appeared enlarged. The remaining central retino-recipient nuclei had the same distribution of retinal input as the control animals.Supported in part by NIH Research Grants EY-01208, EY-01730 and EY-07013, and in part by HD02274  相似文献   

13.
Nicotinamide adenine dinucleotide phosphate-diaphorase (NADPHd) histochemical techniques were used to identify neurons synthesizing nitric oxide in the lateral septum of the guinea pig. Double immunocytochemical procedures were used to detect neurons immunoreactive fof calbindin-D28k and enkephalinergic fibers which project to the lateral septum. The present data demonstrate that (1) the neurons containing NADPH diaphorase and the neurons immunoreactive for calbindin-D28k are observed in discrete regions of the lateral septum; (2) these populations overlap in various areas of the lateral septum including its dorsal and mediolateral parts; (3) NADPH diaphorase and calbindin-D28k are colocalized in neurons located in the overlapping areas; (4) neurons identified by the presence of calbindin-D28k, NADPH diaphorase or both substances, are surrounded by enkephalinergic fibers. These observations indicate the chemical heterogeneity of the lateral septum and suggest that the enkephalinergic hypothalamo-septal tract does not preferentially contact a subpopulation of neurons.  相似文献   

14.
本研究运用荧光金(FG)逆行束路追踪与5-HT1A受体免疫荧光组织化学染色技术相结合,观察了大鼠腰骶髓后连合核(DCN)和中间带外侧核(IML)内感受盆腔内脏伤害性信息并向外侧臂旁核(LPB)发出投射的神经元呈5-HT1A受体免疫反应阳性。将FG注入一侧LPB后,可在腰骶节段(L6-S2)观察到大量的FG逆标神经元,这些FG逆标神经元主要集中于DCN和IML内,以同侧为主;5%福尔马林注入大鼠结肠后,Fos蛋白阳性神经元主要分布于腰骶髓DCN和IML,以同侧为主,在同侧脊髓背角I层、II层和深层也有少量的分布。另外,在腰骶髓DCN和IML内,还可观察到大量5-HT1A受体阳性的神经元胞体、纤维和终末,同时有部分Fos蛋白阳性的FG逆标神经元呈5-HT1A受体阳性。上述结果提示,大鼠腰骶髓DCN和IML内的5-HT能终末可能对盆腔内脏伤害性信息的传递发挥调控作用。  相似文献   

15.
Several recent studies have reported loss of neurons in the nucleus basalis in Alzheimer's disease. However, few detailed studies of the normal distribution of these neurons in the human brain have appeared. We have used Nissl staining and acetylcholinesterase histochemical staining of the human basal forebrain, alone or in combination to identify the organization of the nucleus basalis and associated cell groups, (or collectively, the magnocellular basal nucleus) in the normal human brain. The magnocellular basal nucleus includes a series of clusters of neurons and scattered perikarya extending from the medial septum and diagonal band nucleus rostrally, through the substantia innominata to the furthest caudal extent of the globus pallidus. This distribution is similar to that which has been described in the monkey. Furthermore, acetylcholinesterase-positive fibers in the human brain are seen in the two major pathways that have been identified as carrying magnocellular basal nucleus axons to the cerebral cortex in other species.These observations suggest that the topographic organization of the magnocellular basal projection to cerebral cortex in other species probably exists in man as well. It will therefore be important in future studies of the fate of these neurons in neurological degenerative diseases to assess the loss of neurons in the different components of the magnocellular basal nucleus in relation to the clinical evidence for dysfunction in the cortical areas which they innervate.  相似文献   

16.
The present study examined uncrossed and crossed projections from upper cervical segments to the vestibular nuclei, and the relationship between the afferents and vestibulospinal neurons in the rat. Afferent axons were labeled following unilateral injections of biotinylated dextran into the C2 and C3 segments, while vestibulospinal neurons were labeled following unilateral injections of cholera toxin subunit B into the same segments. The terminals of uncrossed afferents were distributed in the entire area of the rostrocaudal extent of the lateral vestibular nucleus (LV). In the magnocellular part (MVmc) of the medial vestibular nucleus (MV), they were seen near the parvocellular part (MVpc) of the MV at caudal levels. In the MVpc, terminals were seen laterally and ventromedially, close to the border of the MVmc. At caudal levels of the caudal part (MVc) of the MV, they were distributed within the groups of labeled neurons in the middle and lateral areas. In the descending vestibular nucleus (DV), terminals were abundant dorsally and laterally in the rostral two-thirds. The distribution of contralateral cervical afferents was similar to that of ipsilateral afferents. The terminals of ipsilateral and contralateral cervical afferents were seen in contact with vestibulospinal neurons in the DV. The present study demonstrates bilateral input from upper cervical segments to the LV, DV, and all subdivisions of the MV. The input to the LV would contribute to the tonic neck reflex or cervicovestibulospinal reflex.  相似文献   

17.
Anterograde tracing studies were conducted in order to identify efferents from the arcuate nucleus, which contains the hypothalamic opiocortin neuronal pool. Phaseolus vulgaris leucoagglutinin (PHA-L) was stereotaxically iontophoresed into the arcuate nucleus and the terminal fields emanating from the labelled perikarya were identified immunocytochemically. PHA-L-immunoreactive (-ir) fibers were identified in nucleus accumbens, lateral septal nucleus, bed nucleus of the stria terminalis, medial and lateral preoptic areas, anterior hypothalamus, amygdaloid complex, lateral hypothalamus, paraventricular nucleus, zona incerta, dorsal hypothalamus, periventricular gray, medial thalamus and medial habenula. In the brainstem, arcuate terminals were identified in the periaqueductal gray (PAG), dorsal raphe nucleus (DRN), nucleus raphe magnus (NRM), nucleus raphe pallidus, locus coeruleus, parabrachial nucleus, nucleus reticularis gigantocellularis pars alpha, nucleus tractus solitarius and dorsal motor nucleus of the vagus nerve. Dual immunostaining was used to identify the neurochemical content of neurons in arcuate terminal fields in the brainstem. Arcuate fiber terminals established putative contacts with serotonergic neurons in the ventrolateral PAG, DRN and NRM and with noradrenergic neurons in periventricular gray, PAG and locus coeruleus. In the PAG, arcuate fibers terminated in areas with neurons immunoreactive to substance P, neurotensin, enkephalin and cholecystokinin (CCK) and putative contacts were identified with CCK-ir cells. This study provides neuroanatomical evidence that putative opiocortin neurons in the arcuate nucleus influence a descending system which modulates nociception.  相似文献   

18.
The widespread distribution of neurons containing alpha-atrial natriuretic polypeptide-like immunoreactivity in the rat brain was demonstrated using radioimmunoassay and immunohistochemistry in conjunction with specific antisera. The highest concentrations of alpha-atrial natriuretic polypeptide-like immunoreactivity were in the hypothalamus and septum, with low but still appreciable concentrations in the mesencephalon, cerebral cortex, olfactory bulb and thalamus by radioimmunoassay. Immunohistochemical studies clearly showed that the perikarya of immunoreactive neurons are most prevalent in the ventral part of the lateral septal nucleus, periventricular preoptic nucleus, bed nucleus of the stria terminalis, periventricular and dorsal parts of the paraventricular hypothalamic nucleus, ventromedial nucleus, dorsomedial nucleus, arcuate nucleus, median mamillary nucleus, supramamillary nucleus, zona incerta, medial habenular nucleus and the periaqueductal grey matter. Scattered neurons were seen in the cingulate cortex, endopiriform nucleus, lateral hypothalamic area, and pretectal and dorsal thalamic areas. In addition to the areas mentioned above, high concentrations of immunoreactive varicose fibers were seen in the glomerular layer of the olfactory bulb, external layer of the median eminence, central to paramedian parts of the interpeduncular nucleus and the paraventricular hypothalamic nucleus. The globus pallidus, medial and central amygdaloid nuclei, dorsal raphe, dorsal parabrachial nucleus, locus coeruleus, vagal dorsal motor nucleus, solitary nucleus and some circumventricular organs, including the subfornical organ and organum vasculosum laminae terminalis, contained considerable numbers of immunoreactive varicose fibers. In dehydrated rats and homozygous Brattleboro rats, the pattern of alpha-atrial natriuretic polypeptide-immunoreactive neurons and varicose fibers was qualitatively similar to that seen in normal conditioned rats. This study gives an atlas of the distribution of the alpha-atrial natriuretic polypeptide-containing neuronal system in the rat brain and provides the groundwork for studying the influence of this new peptide on various brain functions.  相似文献   

19.
Fang PC  Stepniewska I  Kaas JH 《Neuroscience》2006,143(4):987-1020
Connections of motor areas in the frontal cortex of prosimian galagos (Otolemur garnetti) were determined by injecting tracers into sites identified by microstimulation in the primary motor area (M1), dorsal premotor area (PMD), ventral premotor area (PMV), supplementary motor area (SMA), frontal eye field (FEF), and granular frontal cortex. Retrogradely labeled neurons for each injection were related to architectonically defined thalamic nuclei. Nissl, acetylcholinesterase, cytochrome oxidase, myelin, parvalbumin, calbindin, and Cat 301 preparations allowed the ventral anterior and ventral lateral thalamic regions, parvocellular and magnocellular subdivisions of ventral anterior nucleus, and anterior and posterior subdivisions of ventral lateral nucleus of monkeys to be identified. The results indicate that each cortical area receives inputs from several thalamic nuclei, but the proportions differ. M1 receives major inputs from the posterior subdivision of ventral lateral nucleus while premotor areas receive major inputs from anterior parts of ventral lateral nucleus (the anterior subdivision of ventral lateral nucleus and the anterior portion of posterior subdivision of ventral lateral nucleus). PMD and SMA have connections with more dorsal parts of the ventral lateral nucleus than PMV. The results suggest that galagos share many subdivisions of the motor thalamus and thalamocortical connection patterns with simian primates, while having less clearly differentiated subdivisions of the motor thalamus.  相似文献   

20.
The distribution of neurons in diencephalon and brainstem which project upon the hippocampal formation has been analyzed in adult rats by the injection of horseradish peroxidase into different parts of the hippocampus and dentate gyrus and the related retrohippocampal structures, including the subicular complex and the entorhinal cortex. Any large injection of horseradish peroxidase inlo the hippocampal region results in the retrograde labeling of some neurons in each of the following structures: in the thalamus—the nucleus reuniens, the parataenial and paraventricular nuclei, the anterodorsal and antermedial nuclei, and the laterodorsal and lateral posterior nuclei; in the hypothalamus, septum and preoptic region—the medial septal nucleus and the diagonal band of Broca, the substantia innominata, the lateral preoptic area, the magnocellular preoptic nucleus, and the anterior amygdaloid area, the dorsomedial hypothalamic nucleus, the lateral and posterior hypothalamic areas, the ventral premammillary nucleus, the supramammillary region, and parts of the tuberomammillary and lateral and medial mammillary nuclei: in the brainstem: the ventral tegmental area, the substantia nigra, the interpeduncular and interfascicular nuclei, the dorsal and median nuclei of the raphe, the dorsal and laterodorsal tegmental nuclei, the locus coeruleus, the central gray, and certain of the tegmental reticular fields.It is clear that these afferents to the hippocampal formation do not comprise a single, homogeneous system, and that their terminations within the hippocampal region are not restricted to a particular topographic level (i.e. septal, intermediate or temporal).  相似文献   

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