富硒酵母对大鼠肝纤维化形成的影响

目的 评价富硒酵母对大鼠肝纤维化形成的影响。方法 用４０％ＣＣＩ４３个月诱导大鼠肝纤维化形成，同时每天分别给邓定硒酵母５０ｍｇ／ｋｇ或秋水仙碱０．１ｍｇ／ｋｇ。检测大鼠血甭丙氨酸氨基转换酶（ＡＬＴ）、白蛋白（ＡＬＢ）层粘蛋白（ＬＮ）、透明质酸（ＨＡ）和Ⅳ型原的（ＣⅣ）水平以及肝脏纤维化程度和羟脯氨酸（ＨＹＰ）的含此评价所用药物对大鼠肝纤维化形成的影响。结果 富硒酵母对ＣＣＩ４所致大鼠血清ＡＬＴ，Ｌ     

富硒条件对杭白菊硒含量的影响

探讨了不同富硒浓度对杭白菊硒吸收、硒有机化和感官色泽的影响，并讨论了不同喷洒量、水质纯度、喷洒次数和茬数以及杭白菊不同结构组分对小洋菊富硒的影响，不同取点对硒喷洒和吸收的均匀性衡量的影响，干菊花干燥温度对硒损失的影响。     

不同泡制工艺对蔬菜硒富集和转化的影响

对白萝卜进行了先浸泡后发酵富硒工艺和直接富硒发酵工艺的比较研究 ,得出了富硒泡菜中总硒和有机硒的影响规律 .对比了消化前后的硒标准溶液工作曲线 ,确定了本试验条件下的泡菜发酵终点 ,讨论了不同的适宜泡制的蔬菜品种的差异对富硒的影响规律 ,得出了实用的富硒泡菜工艺参数 .     

加工条件对富硒绿叶蔬菜汁品质稳定性的影响

探讨了 3种富硒绿叶蔬菜在制汁过程中 2个主要品质指标 (叶绿素含量和硒含量 )的变化规律 .着重研究了蔬菜汁漂烫、护色后杀菌等加工条件对叶绿素含量、总硒含量和有机硒比例的影响 ,讨论了产前农艺富锌与产后浸泡富锌对青菜叶绿素含量的影响以及复合菜汁的叶绿素保存及贮藏期分层问题 .     

富硒蔬菜在低真空脱水方式下硒保存规律及优化研究

对青椒、茄子和黄瓜三种适宜脱水的富硒蔬菜进行了低真空脱水，得到了脱水温度对脱水蔬菜含硒总量和有机硒比例的影响曲线；采用响应曲面法（ＲＳＭ），对富硒青椒的预烫时间和ｐＨ值以及低真空脱水温度条件作了优化设计，得出了较佳的有机硒和色素含量的工艺条件。     

锌硒宝对治疗慢性前列腺炎症状的影响

慢性前列腺炎是泌尿男科门诊的常见病,为了探讨口服锌硒宝对慢性前列腺炎患者的影响,我们从2000年10月到2001年6月对40例慢性前列腺炎口服锌硒宝患者进行临床观察,现将有关情况报告如下.     

硒对淋巴细胞抗大肠癌作用影响的基础和临床研究

目的 :探讨硒对淋巴细胞抗大肠癌的作用。方法 :包括实验与临床二部分 ,以半胱氨酸硒作为影响因素、人 T淋巴细胞为效应细胞 ,人结肠癌细胞 (L o Vo细胞株 )为靶细胞 ;采用 MTT比色法、凋亡细胞荧光计数检测凋亡细胞的数量 ;同时检测上清液中 TNF- α(肿瘤坏死因子 α)的含量。然后 ,于 1998年 3月至 1999年 3月 ,将 40例大肠癌患者 ,随机分成对照组和治疗组分别给予一般酵母和酵母硒 (6 0 0 μg/ d)持续两周 ,观察治疗前后血硒水平、T细胞亚群和免疫球蛋白 ,以及细胞因子 αTNF的变化。结果 :半胱氨酸硒在一定的浓度范围内 (0 .5 μg/ m l～ 1.0 μg/ m l)可增强人 T淋巴细胞的抗肿瘤活性(P <0 .0 5 ) ,促进人结肠癌细胞的凋亡 ,且肿瘤细胞的凋亡比例上升 (P <0 .0 5 ) ;T淋巴细胞抗肿瘤作用的增强与上清液中的 TNF- α的增高有关 (P <0 .0 5 )。大肠癌患者经补硒后 ,血硒由 (0 .76± 0 .17) μm ol/ L 上升到 (0 .99± 0 .2 3) μmol/L,P <0 .0 5 ;CD4由 (2 4.0 9± 4.10 ) %上升到 (2 7.95± 4.91) %,CD8由 (2 0 .18± 2 .48) %上升到 (2 3.0 9± 4.47) %,P <0 .0 5 ;血清中 Ig M从 (83.7± 2 3.37) mg上升到 (10 3.6 9± 35 .49) mg,P <0 .0 5。结论 :硒可提高淋巴细胞的抗肿瘤作用 ,改善机体的免疫     

硒锌对培养兔和人胚软骨细胞生长的影响

本实验在培养液中加入一定量的硒，锌对一代兔和人胚关节软骨细胞进行培养１９２小时。结果显示：补充适量的硒或锌均能促进培养软骨细胞的生长代谢，但硒过量则损害于软骨细胞，若同时补给适量的锌和硒，其效果有相加的作用。补锌和补硒的效果可能为硒＋锌＞锌＞硒。同时表明体外培养软骨细胞的基质代谢能很快进入平衡状态。人胚软骨细胞生长慢于兔，基质胶原代谢慢于蛋白多糖     

几种蔬菜的硒结合规律和农艺优化研究

对青椒、茄子、黄瓜、菜豆、生菜和小葱进行了不同浓度的无机硒喷洒处理试验，得出了不同浓度对硒吸收及硒有机化的影响。并讨论了不同生育期硒处理对黄瓜富硒的影响、硒吸收后在黄瓜果皮和果肉中的分布以及硒测试时在黄瓜压榨汁和渣上的硒分布。采用响应面优化设计得出了黄瓜的最佳喷洒农艺方案。     

藤梨根对人脑胶质瘤U251细胞的影响及其机制

目的 探讨藤梨根对人脑胶质瘤U251细胞的作用及其机制.方法 常规培养人脑胶质瘤细胞株U251,分别加入不同浓度(50、100、200 mg/L)藤梨根作用48 h后细胞计数试剂盒(CCK-8)法检测U251的细胞活性;取50 mg/L藤梨根作用48 h后的细胞,反转录-聚合酶链反应(RT-PCR)法检测信号转导和转录活化因子3(STAT3) mRNA的表达,Western blot检测STAT3蛋白的表达.结果 藤梨根作用48 h后U251细胞凋亡率明显增加,不同浓度的凋亡率分别为50 mg/L:(26.51士1.30)％、100 mg/L:(55.92 ±2.40)％、200mg/L:(63.69±2.70)％;50 mg/L藤梨根处理后的U251细胞中STAT3 mRNA和蛋白表达水平均下调,检测其中相关蛋白表达结果显示,藤梨根处理组中磷酸化STAT3(p-STAT3)和B淋巴细胞瘤/白血病-2(bcl-2)的表达下调,而bcl-2相关X蛋白(bax)的表达上调.结论 藤梨根可以抑制脑胶质瘤细胞的增殖,其机制可能与藤梨根调控STAT3信号通路有关.     

蒲葵子提取物对膀胱癌细胞增殖凋亡的影响及其相关机制

目的:探讨蒲葵子提取物对膀胱癌细胞增殖、凋亡的影响及其相关机制。方法:将T24细胞分别用含蒲葵子提取物且终浓度分别为0、25、50、100 mg/L的培养液培养,分别记为对照组和蒲葵子低、中、高剂量组。用细胞计数试剂盒8(CCK-8)检测细胞存活率;克隆形成实验检测细胞克隆形成数量;流式细胞术检测细胞凋亡;蛋白质印迹法...     

Effect of Positioning of the ROI on BMD of the Forearm and Its Subregions

Inconsistent positioning of patients and region of interest (ROI) is known to influence the precision of bone mineral density (BMD) measurements in the spine and hip. However, it is unknown whether minor shifts in the positioning of the ROI along the shaft of the radius affect the measurement of forearm BMD and its subregions. The ultradistal (UD-), mid-, one-third, and total radius BMDs of 50 consecutive clinical densitometry patients were acquired. At baseline the distal end of the ROI was placed at the tip of the ulnar styloid as usual, and then the forearm was reanalyzed 10 more times, each time shifting the ROI 1?mm proximally. No corrections for multiple comparisons were necessary since the differences that were significant were significant at p?<?0.001. The UD-radius BMD increased as the ROI was shifted proximally; the increase was significant when shifted even 1?mm proximally (p?<?0.001). These same findings held true for the mid- and total radius bone density, though the percent increase with moving proximally was significantly greater for the UD radius than for the other subregions. However, there was no significant change in the one-third radius BMD when shifted proximally 1–10?mm. Minor proximal shifts of the forearm ROI substantially affect the BMD of the UD-, mid- and total radius, while having no effect on the one-third radius BMD. Since the one-third radius is the only forearm region usually reported, minor proximal shifts of the ROI should not influence forearm BMD results significantly.     

羟丙基甲基纤维素对磷酸钙水泥注射性能的影响

[目的]研究羟丙基甲基纤维素对磷酸钙骨水泥可注射性的影响。[方法]用注射能力系数法和稠度测定法对含不同浓度HPMC（0～4％）的CPC的注射性能进行评价。[结果]增加HPMC的浓度，CPC的注射性能也增加。HPMC在2％时，注射性能最好，注射能力系数达95％，稠度最低，对水泥的固化时间无明显影响。HPMC超过3％后注射性能开始下降，至4％时注射能力系数降至90％。[结论]适当浓度的HPMC可以很好的改善CPC的流变学性能，增加其可注射性能，适于微创骨科的应用。     

Systematic review and meta-analysis on laparoscopic cystectomy in bladder cancer

BackgroundThis study aimed to systematically evaluate the efficacy of laparoscopic radical cystectomy (LRC) surgical therapy in patients with bladder cancer (BC), and to provide evidence for the clinical treatment of BC.MethodsThe Embase, Ovid, PubMed, Medline, Springer, and Web of Sciences database were searched to screen articles with clinical controlled trials on LRC treatment of BC. The Cochrane Handbook 5.0.2 software and Review Manager 5.3 software were adopted to evaluate the risk of bias and to perform a meta-analysis of the included articles in this study.ResultsA total of 12 articles were obtained, including 1,283 research cases. The meta-analysis results showed that relative to the control group (Ctrl), the observation group (Observ group) had significantly lower intraoperative blood loss (IBL) after LRC [mean difference (MD) =−458.75; 95% confidential interval (CI): −505.75 to −411.76; Z=19.13; P<0.00001], blood transfusion rate (BTR) (odds ratio =0.36; 95% CI: 0.13–0.94; Z=2.08; and P=0.04), use of analgesics (MD =−24.53; 95% CI: −39.04 to −10.01; Z=3.31; and P=0.0009), and incidence of postoperative complications (Risk ratio =0.58; 95% CI: 0.39–0.85; Z=2.77; and P=0.006). However, and the length of hospital stay could not be shortened (MD =–2.43; 95% CI: –4.83 to –0.02; Z=1.98; and P=0.05).DiscussionLRC treatment of BC could effectively reduce the amount of IBS, and lower the intraoperative BTR, use of analgesics, and incidence of postoperative complications. Therefore, it could be used in the clinical surgical treatment of BC patients.     

Protective Effects of Social Support Content and Support Source on Depression and Its Prevalence 6 Months after Wenchuan Earthquake

A magnitude 8.0 earthquake struck China's southwestern Sichuan province on 12 May 2008. The reported rates of depression symptoms across studies were not consistent, and its protective factors were unknown. This study collected data from Wenchuan earthquake survivors to estimate the prevalence of depression and explore the protective effects of social support and support source on depression. A randomized sampling cross‐sectional survey based on community was conducted in January 2009, in Mianzhu and Anxian counties, and 633 survivors were entered into the study. The Chinese edition of the Center for Epidemiologic Studies Depression Scale and the Social Support Rating Scale were used to investigate depression and social support. The prevalence of probable depression in adults 6 months after the Wenchuan earthquake was 22.9% (145/633). Total social support, subjective support, support use and support from family members, neighbours and organizations negatively predicted depression. According to the results, depression was common 6 months after this major disaster. Total social support, subjective support, support use and support from family members, neighbours and organizations were all protective factors for depression after a major disaster. Copyright © 2014 John Wiley & Sons, Ltd.     

饲料含水率对淀粉糊化及蛋白变性温度的影响

在过量水的条件下,用DSC测定了小麦淀粉、小麦面粉、大豆粕、鱼粉,以及用这些组分配合而成的对虾饲料等试样加热时的吸热曲线,并以此辩认了这些原料中所含淀粉及蛋白质的吸热峰值。在不同的水分含量条件下,测定了这些峰值的变化,其结果被引用于解释常用制粒条件(水分17%,温度93℃)下所得颗粒饲料的糊化度仅为35%左右的原因。     

基于失效模式与效应分析的综合医院住院患者自杀风险管理

目的 探讨失效模式与效应分析在综合医院预防住院患者自杀管理中的应用效果。方法成立跨专业的多学科小组,绘制预防住院患者自杀流程图,对预防患者自杀管理过程中可能出现的失效模式进行严重度、发生的可能性、探测的可能性评分,根据危害评分矩阵评估风险优先指数,针对风险优先指数＞125分的5项失效模式进行改进。比较实施前后的风险优先指数和住院患者自杀发生率。结果实施后,住院患者自杀风险总体风险优先指数由（238.96±67.03）分下降为(73.48±6.05)分;住院患者自杀率由4.8/10万下降至2.1/10万。结论基于失效模式与效应分析的风险管理可有效降低综合医院住院患者自杀风险优先指数,可早期识别和干预有自杀倾向的住院患者,保障患者安全。     

富血小板纤维蛋白对人脂肪干细胞增殖和成脂分化的影响

目的 探讨自体富血小板纤维蛋白(platelet-rich fibrin,PRF)对体外培养人脂肪来源干细胞(adipose-derived stem cells,ADSCs)增殖及成脂分化的影响.方法 将自愿捐献由脂肪抽吸术获取的脂肪组织进行分离培养ADSCs并鉴定.将第3代ADSCs分为空白对照组和1个PRF膜片组(1PRFM组)和2个PRF膜片组(2PRFM组).倒置显微镜观察细胞生长情况,培养后1、2、3、4、5、6、7d采用四甲基偶氮噻唑蓝比色法(MTT)法检测细胞增殖活性.在第3、5、7、9、11和14天时采用油红O染色法检测细胞成脂分化情况.结果 随着PRFM剂量的增加,细胞增殖数量和成脂率增加,3组差异具有显著统计学意义.结论 PRF能明显促进ADSCs增殖和成脂分化,可以作为自体材料应用于脂肪组织工程的研究.