口腔龋病与非龋病部位牙菌斑中幽门螺杆菌检出的比较

本文采用快速尿素酶试验方法,检测了４９例龋病患者龋病及非龋病部位牙菌斑中尿素酶活性,结果显示龋病与非龋病牙菌斑中均可检出Ｈｐ的存在,而且龋病牙菌斑中Ｈｐ的检出率明显高于非龋病牙菌斑中Ｈｐ的检出率（ｐ＜０．０５）,表明Ｈｐ可寄生于口腔牙菌斑中,特别是龋病形成的牙菌斑中其存在意义仍需要进一步研究．     

正常人,龋病和牙髓炎患者牙髓组织中一氧化氮含量的检…

目的：正常人，龋病和牙髓炎病患者牙髓组织中一氧化氮（Ｎｉｔｒｉｃｏｘｉｄｅ，ＮＯ）含量进行检测，初步探讨ＮＯ在牙髓组织自身修复中的作用。方法：采用荧光分光光度法检测其中亚硝酸根（ＮＯ２）含量，以间接确定ＮＯ含量。结果：正常牙髓组织中ＮＯ无性别差异（Ｐ〉０．０５），浅龋组与对照组ＮＯ无显著性差异（Ｐ〉０．０５），而深龋伴慢性牙髓炎组ＮＯ含量则显著高于对照组（Ｐ〈０．０５）。结论：牙髓组织中ＮＯ含量的     

牙周炎患者龈沟液中乳酸脱氢酶水平分析

目的：探讨龈沟液中乳酸脱氢酶（ＬＤＨ）水平对于慢性成人牙周炎患者诊断及预后观察的意义。方法：酶动力学方法。结果：患病部位和健康部位龈沟液中ＬＤＨ水平有非常显著差异（Ｐ〈０．００１）。探诊深度和龈沟液中ＬＤＨ水平呈正相关（Ｐ〈０．０５）。附着丧失水平和龈沟液中ＬＤＨ水平呈正相关（Ｐ〈０．０５）。结论：龈沟液中ＬＤＨ水平对于慢性成人牙周炎的诊断和疗效监测具有一定的临床意义。     

牙周炎患者红细胞免疫状态的初步分析

目的：通过对牙周炎患者外周血红细胞Ｃ３ｂ受体花环率（ＲＢＣ－Ｃ３ｂＲＲ）和红细胞免疫复合物花环率（ＲＢＣ－ＩＣＲ）的检测,观察青少年牙周炎（ＪＰ）、快速进展型牙周炎（ＲＰＰ）、成人牙周炎（ＡＰ）的红细胞免疫功能状况。方法：采用红细胞酵母菌花环试验方法。结果：ＪＰ、ＲＰＰ患者红细胞免疫粘附力明显低于正常人。ＪＰ、ＲＰＰ患者ＲＢＣ－Ｃ３ｂＲＲ分别为（１０．０３±０．９２）％、（１２．２０±０．９４）％,与对照组（１５．９０±１．７７）％有显著差异（Ｐ＜０．００１,Ｐ＜０．０１）;ＲＢＣ－ＩＣＲ分别为（２７．８９±１．７７）％、（２７．０１±１．７９）％,与对照组（３０．６４±３７）％有显著性差异（Ｐ＜０．０１）;ＡＰ患者ＲＢＣ－Ｃ３ｂＲＲ为（１６．０２±１．２５）％、ＲＢＣ－ＩＣＲ为（３０．０４±１．７３）％,与对照组无显著性差异（Ｐ＞０．０５）。结论：提示ＪＰ、ＲＰＰ的发病机理可能与宿主的红细胞免疫功能低下有关。     

儿童口腔卫生状况与龋病、牙龈炎的关系

目的：研究儿童口腔卫生状况与龋病、牙龈炎的关系．方法：用患龋率、龋均（ｄｆｔ）、龋蚀重度指数（ＣＳＩ）、牙龈指数（ＰＭＡ）及口腔卫生指数（ＯＨＩ）对上海医科大学附属幼儿园１２０名５～６岁儿童的龋病、牙龈炎及口腔卫生状况进行调查．结果：ＯＨＩ、患龋率、ｄｆｔ、ＣＳＩ在男女间无显著性差异（ｐ＞０．０５）．ＯＨＩ与患龋率、ｄｆｔ、ＣＳＩ间无相关关系（ｒ分别为０．１１７１,０．０２７９,０．０３１０,Ｐ分别为０．２０２６,０．７６２０,０．７３６８）．ＯＨＩ与ＰＭＡ有显著相关性（ｒ＝０．３０３１,Ｐ＝０．０００８）．结论：口腔卫生状况与牙龈炎的发生关系密切．菌斑程度并不能完全反映龋蚀状况．改善口腔卫生状况对预防龋病、牙龈炎的发生具有重要意义。     

正常人、龋病和牙髓炎患者牙髓组织中一氧化氮含量的检测分析

目的：对正常人、龋病和牙髓炎患者牙髓组织中一氧化氮（Ｎｉｔｒｉｃｏｘｉｄｅ，ＮＯ）含量进行检测，初步探讨ＮＯ在牙髓组织自身修复中的作用。方法：采用荧光分光光度法检测其中亚硝酸根（ＮＯ２－）含量，以间接确定ＮＯ含量。结果：正常牙髓组织中ＮＯ无性别差异（Ｐ＞０．０５），浅龋组与对照组ＮＯ无显著性差异（Ｐ＞０．０５），而深龋伴慢性牙髓炎组ＮＯ含量则显著高于对照组（Ｐ＜０．０５）。结论：牙髓组织中ＮＯ含量的变化与其炎症反应有一定的联系，ＮＯ可能在牙髓炎症和自身修复过程中起到一定的作用。     

口腔粘膜下纤维性变组织中内皮素1的免疫组织化学和定量研究

目的：探讨ＥＴ－１在口腔粘膜下纤维性变（ＯＳＦ）发病机制中的作用。方法：采用免疫组化染色ＳＡＢＣ法和图像分析技术,对ＯＳＦ早、中、晚期各１０例、口腔扁平苔藓（ＯＬＰ）１０例以及正常人１０例的颊粘膜组织中ＥＴ－１表达进行定量分析。结果：①ＯＳＦ组织中ＥＴ－１免疫阳性物质超量表达,正常及ＯＬＰ组织中ＥＴ－１表达微弱;②ＯＳＦ早、中、晚期组织中ＥＴ－１含量显著高于正常（Ｐ〈０．０１）,且早、中期显著高于晚期（Ｐ〈０．０１）;③ＯＳＦ早、中期组织ＥＴ－１含量显著高于ＯＬＰ（Ｐ〈０．０１）,晚期间质ＥＴ－１含量显著高于ＯＬＰ（Ｐ〈０．０５）,上皮ＥＴ－１含量两者差异不显著（Ｐ〉０．０５）;④口腔粘膜中上皮和间质的ＥＴ－１含量呈显著正相关（Ｐ〈０．０５）。结论：ＥＴ－１在ＯＳＦ的表达具有特异性,且可能影响ＯＳＦ病变的发生发     

牙周炎患者龈组织中的自氧化作用与临床指数的相关性研究

目的：探讨内源性超氧基（Ｏ２）损伤在慢性成人牙周炎（ＡＰ）发生发展过程中的作用。方法：采用化学发光法、氧化法和比色法对４２例ＡＰ患者龈组织中的超氧基（Ｏ２）、超氧化物歧化酶（ＳＯＤ）活性和过氧化脂质产物（ＭＤＡ）含量进行测定。并将反映自氧化作用的ＳＯＤ．ＭＤＡ与临床指数进行直线相关及多元线形回归分析。结果：实验组与对照组 Ｏ２、ＳＯＤ、 ＭＤＡ活性水平有显著性差异（Ｐ＜ ０． ００１）; ＳＯＤ与周袋深度（ＰＤ）、附着丧失（ＡＬ）呈负相关（Ｐ＜０．００１）;ＭＤＡ与ＰＤ、ＡＬ呈正相关（Ｐ＜０．００１）。结论：氧自由基参与牙周病的病理过程。     

成人牙周炎患者治疗前后龈沟液中谷胱甘肽过氧化物酶水平的变化

目的：了解谷胱甘肽过氧化物酶（ＧＰｘ）在牙周病变过程中的变化及其临床意义。方法：选择２３例成人牙周炎（ＡＰ）患者的４４颗患牙,记录临床指标ＧＩ、ＰＤ、ＡＬ,并测定治疗前后ＧＣＦ－ＧＰｘ水平。结果：ＧＣＦ－ＧＰｘ与ＰＤ、ＡＬ负相关（Ｐ〈０．０５,Ｐ,０．００５）,与ＧＩ无明显相关性。牙周治疗后临床指标均明显下降（Ｐ〈０．００１）,ＧＣＦ－ＧＰｘ水平明显上升（Ｐ〈０．００１）,但ＧＣＦ－ＧＰＸ水平的上     

口腔卫生状况与慢性胃炎关系的调查研究

目的：初步探讨口腔卫生情况与慢性胃炎的关系。方法：对半岛地区城乡２９４５名中老年人的口腔卫生状况进行并抽样测定软垢指数（ＤＩ）、牙石指数（ＣＩ）、菌斑指数（ＰＬＩ）,牙龈指数（ＧＩ）和牙周病指数（ＰＤＩ）,并通过聚合酶链反应（ＰＣＲ）检测菌斑中幽门螺杆菌（Ｈｐ）,通过胃镜检查慢性胃炎发病情况。结果：慢性胃炎组与对照组间各指数检测结果差别显著（Ｐ〈０．０１）;两组菌斑Ｈｐ阳性率分别为８１．１％和３７     

高压氧对口腔牙菌斑幽门螺杆菌的作用

目的：寻找一种较为理想的根除口腔幽门螺杆菌（Hp)的方法。方法：将120例口腔牙菌斑Hp-PCR检测阳性的Ⅱ度牙周炎患者分为A、B、C、D4组，治疗1、3、6月后分别对所有患者牙周参数和龈下菌斑进行Hp-PCR检测并比较检测结果。结果：4组Hp阳性率分别为96.7%、80.0%、73.3%和0，有显著性差异（P＜0.05)，其中以D组最低（P＜0.05)。结论：高压氧对口腔Hp有明显抑制或杀灭作用，高压氧配合治疗，有助于口腔Hp的根除和与Hp有关的疾病的治疗。     

口腔卫生与慢性胃炎关系的临床研究

目的 :为探讨口腔卫生与慢性胃炎的关系。方法 :76例胃粘膜幽门螺杆菌 (Helicobacterpylori,Hp)检测阳性慢性胃炎患者为慢性胃炎组 ,72例健康自愿者组成对照组 ,对所有受检者进行口腔卫生检查 ,并对其牙菌斑Hp进行检测 ,比较两组的差别 ;慢性胃炎组随机分成A、B两组后接受抗Hp治疗 ,A组同时进行口腔治疗与口腔教育 ,B组为对照 ,3月后观察抗Hp治疗的疗效 ,比较两组的差别。 结果 :慢性胃炎组口腔卫生状况明显较对照组差 (P<0 .0 1) ,且口腔卫生状况与慢性胃炎程度有关 (P <0 .0 5 ) ;牙菌斑Hp检测结果亦显示口腔卫生愈差、慢性胃炎愈严重 ,Hp阳性率愈高。 3月后A组的疗效明显优于B组。 结论 :口腔卫生好坏与慢性胃炎的发病密切相关 ,Hp寄生于牙菌斑可能是导致慢性胃炎发病和复发的一个重要因素。     

牙菌斑和胃幽门螺杆菌感染联合治疗对复发性口腔溃疡疗效的影响

目的：探讨对牙菌斑和胃幽门螺杆菌（helicobacter pylori,Hp)感染联合治疗对复发性口腔溃疡（recurrent oral ulcer,ROU)疗效的影响。方法：对198例牙菌斑标本Hp-PCR检测阳性患者进行分组治疗：胃镜检查无胃病的ROU患者（A组）仅接受针对ROU的治疗;伴有胃病的患者随机分成B、C组,B组接受抗Hp治疗,C组在清除牙菌斑的同时接受A、B组的联合治疗。观察根除Hp对口腔溃疡疗效及复发的影响。结果：198例ROU中,130例（65．66％）患有不同类型的慢性胃炎和消化性溃疡（peptic ulcer,PU),对口腔溃疡和胃病的疗效以及Hp的清除效果均以C组最佳（P＜0．05）,C组ROU的复发率明显低于A、B组（P＜0．01）。结论：牙菌斑和胃粘膜联合治疗Hp可以提高ROU的疗效,减少其复发;ROU的发病可能与Hp感染有关。     

慢性胃病患者口腔与胃内幽门螺杆菌研究

目的：研究慢性胃病患者口腔中是否存在幽门螺杆菌（Hp）,并分析胃病患者牙周状况与幽门螺杆菌的关系。方法：依据特异的尿素酶C基因和cagA基因设计引物,建立聚合酶链反应（PCR）方法。检测65例慢性胃病患者不同牙位龈上和龈下菌斑中的Hp。结果：65例慢性胃病患者胃黏膜标本中,PCR检测阳性标本58例（89．3％）,龈下菌斑Hp阳性率（47．7％）,高于龈上菌斑中的Hp阳性率（26．2％）。牙周袋深度（PD）≥4mm部位菌斑中Hp检出率显著高于PD〈4mm的部位（P〈0．05）。结论：口腔Hp是慢性胃病患者Hp感染的重要来源。     

Dental diseases in subjects with beta-thalassemia major

Abstract – Objectives: The aim of this study was to determine whether β‐thalassemia major is associated with an increase in the severity of periodontal disease and dental caries. Methods: A total of 61 thalassemia patients and 63 healthy controls (age range = 6–18 years) were examined for plaque deposits, gingivitis, periodontitis and dental caries using Silness and Löe plaque index (Pl.I), Löe and Silness gingival index (GI), probing pocket depth (PPD) and DMFT, respectively. Results: There were no significant differences in Pl.I, GI and PPD scores between both thalassemia patients and healthy control groups (P > 0.05). Dental caries was significantly higher in thalassemia patients group (P < 0.0001) compared to the healthy controls. Conclusion: Thalassemia is not associated with increased levels of gingivitis or periodontitis but is associated with higher dental caries experience.     

Relationship between duration of treatment and oral health in adults with intellectual and developmental disabilities

The purpose of this study was to evaluate how dental outcomes changed over time among subjects with intellectual and developmental disabilities (IDD) who were under treatment. This retrospective study included 107 subjects who were treated at a Tufts Dental Facilities clinic. Data from each subject were collected at three time points: initial visit, midpoint visit, and most recent visit. Generalized estimating equations were used to assess the relationship between time in treatment and several outcome variables (cooperation level, hygiene rating, presence of caries, periodontitis, dental pain, and infection). Statistically significant decreases in caries (p < .001) and increases in periodontitis (p = .002) were found over time. Associations between time and other outcome variables were not statistically significant. The prevalence of caries decreased and the prevalence of periodontitis increased over time among patients with IDDs receiving regular comprehensive dental care. Even among patients under routine maintenance, significant oral health problems remain.     

复发性口腔溃疡患者口腔中的幽门螺杆菌

初步探讨复发性口腔溃疡（ｒｅｃｕｒｒｅｎｔｏｒａｌｕｌｃｅｒ,ＲＯＵ）患者的发病与其口腔中的幽门螺杆菌（ｈｅｌｉｃｏｂａｃｔｅｒｐｙｌｏｒｉ,Ｈｐ）之间的关系。方法：通过聚合酶链反应（ｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ,ＰＣＲ）,对每位ＲＯＵ患者口腔中６个不同牙位龈上、龈下共１２份菌斑进行检测,同时设立健康对照组,比较两组检测结果。结果：共检测ＲＯＵ患者７２例,对照组９４例;Ｈｐ阳性率     

Results of a 20-year oral hygiene and prevention programme on caries and periodontal disease in children attended at a private periodontal practice

To cite this article:
Int J Dent Hygiene 9 , 2011; 155–158
DOI: 10.1111/j.1601‐5037.2010.00455.x
Chambrone LA, Chambrone L. Results of a 20‐year oral hygiene and prevention programme on caries and periodontal disease in children attended at a private periodontal practice. Abstract: Objectives: The objective of this study was to evaluate the long‐term effect of an oral hygiene and prevention programme on caries and periodontal disease in a group of children attended at a private periodontal practice. Methods: A total of 50 systemically healthy children, 25 males and 25 females, 03–13 years old, were invited to join a long‐term plaque control programme. All children had no caries and had no evidence of clinical bone loss. The participants were selected amongst children whose parents (mother, father or both) were treated of gingivitis, aggressive periodontitis or chronic periodontitis in a private periodontal practice. Subjects were separated in groups according to their parents’ periodontal diagnosis, i.e., gingivitis, aggressive periodontitis or chronic periodontitis. The following outcomes were evaluated: a) probing depth, b) plaque (PI) and gingival (GI) indexes. The plaque control programme applied consisted of a regular maintenance regime at 6‐to 12‐month interval with an experienced periodontist. Results: In total 30 subjects fulfilled the 20‐year period of maintenance. The mean recall frequency was 6.4 (± 3.1) months, and the mean PI and GI were 0.4 (± 0.3) and 0.3 (± 0.3) respectively. The average rate of caries lesions was 1.0 (± 1.4). None of the patients exhibited clinical or radiographic evidences of alveolar bone loss, and no tooth was lost by caries. In addition, there were no statistically significant differences between groups (P > 0.05). Conclusions: Adequate oral hygiene measures and periodic professional plaque control led to low levels of dental plaque, gingivitis and caries lesions.     

婴幼儿龋病相关因素的初步研究

目的 :了解我国婴幼儿龋病发病的相关因素。方法 :对沈阳市 1860名 8～ 3 6个月的儿童进行患龋情况检查。随机抽取婴幼儿龋组和无龋组各 12 0对母子进行问卷调查及口腔卫生检查。结果 :母亲的文化水平、DMFT及菌斑产酸能力对婴幼儿龋病有影响 (P <0 .0 1) ;婴幼儿期喂养方式与婴幼儿龋病无明显关系 (P >0 .0 5 ) ;断奶时间、含奶瓶或乳头睡觉习惯、甜食习惯与婴幼儿龋病的发生有关系 (P <0 .0 5 ) ;婴幼儿口腔卫生与婴幼儿龋病的发生有关系 (P <0 .0 0 1)。结论 :为了预防婴幼儿龋病应提高母亲素质、宣传正确的饮食方式及注意婴幼儿口腔卫生。