丝光绿蝇抗菌物质在不同pH下抑菌活性的研究

目的探讨丝光绿蝇经针刺体壁诱导产生的抗菌物质在不同的pH条件下的抑菌活性.方法丝光绿蝇三龄幼虫经针刺体壁诱导后48 h,提取其血淋巴,观察其在小同的pH琼脂平板上对溶壁微球菌和金黄色葡萄球菌的抑菌效果;经不同pH缓冲液处理后,观察其对溶壁微球菌的抑菌效果.结果丝光绿蝇幼虫经针刺诱导后48 h的血淋巴,在小同pH琼脂平板上对金黄色葡萄球菌的抑菌效果,pH6.0、6.5环较大、较清晰,随pH增加而环直径减小.对溶壁微球菌的抑菌效果,在pH6.0时,抑菌环直径最大,环径径随pH增加而减小.用不同pH的缓冲液稀释处理时,pH6.0缓冲液稀释后对溶壁微球菌的抑菌环最大,pH9.0缓冲液稀释后对溶壁微球菌的抑菌环最小.结论丝光绿蝇经针刺体壁诱导产生的抗菌物质在酸性条件下比在碱性条件下具有更强的抑菌活性.     

丝光绿蝇抗菌物质在不同pH下抑菌活性的研究

Objective To investigate the antibacterial activity of antibacterial substance in Lucilia seri-cata induced by pricking the skin of maggots(fly s larvae)at different pH conditions. Methods The hemo-lymph from the third instar maggots was collected 48 h after pricking,and the antibacterial effect was tested a-gainst Micrococcus lysodeikticus and Staphylococcus aureus on plates at different pH conditions.The antibacterial effect against M.1ysodeikticus was also detected after the hemolymph、was diluted by different pH buffer solu-tions.The bacteriostatic effects were observed after 24 h and the diameters of clear inhibitory ring were recorded to express antibacterial activity. Results The antibacterial activity of the maggot hemolymph against M.lyso-deiktics and S.aureus was stronger on the plates at pH 6.0 and pH 6.5 than that on the other plates.In the group diluted by pH 6.0 buffer solutions.the antibacterial ring of the maggot hemolymph against M lysodeiktic-us was larger than that in others. Conclusion The results implied that the induced antibacterial substance in Lucilia sericata exhibited stronger antibacterial activity under acidic condition.     

丝光绿蝇抗菌物质在不同pH下抑菌活性的研究

Objective To investigate the antibacterial activity of antibacterial substance in Lucilia seri-cata induced by pricking the skin of maggots(fly s larvae)at different pH conditions. Methods The hemo-lymph from the third instar maggots was collected 48 h after pricking,and the antibacterial effect was tested a-gainst Micrococcus lysodeikticus and Staphylococcus aureus on plates at different pH conditions.The antibacterial effect against M.1ysodeikticus was also detected after the hemolymph、was diluted by different pH buffer solu-tions.The bacteriostatic effects were observed after 24 h and the diameters of clear inhibitory ring were recorded to express antibacterial activity. Results The antibacterial activity of the maggot hemolymph against M.lyso-deiktics and S.aureus was stronger on the plates at pH 6.0 and pH 6.5 than that on the other plates.In the group diluted by pH 6.0 buffer solutions.the antibacterial ring of the maggot hemolymph against M lysodeiktic-us was larger than that in others. Conclusion The results implied that the induced antibacterial substance in Lucilia sericata exhibited stronger antibacterial activity under acidic condition.     

丝光绿蝇抗菌物质在不同pH下抑菌活性的研究

Objective To investigate the antibacterial activity of antibacterial substance in Lucilia seri-cata induced by pricking the skin of maggots(fly s larvae)at different pH conditions. Methods The hemo-lymph from the third instar maggots was collected 48 h after pricking,and the antibacterial effect was tested a-gainst Micrococcus lysodeikticus and Staphylococcus aureus on plates at different pH conditions.The antibacterial effect against M.1ysodeikticus was also detected after the hemolymph、was diluted by different pH buffer solu-tions.The bacteriostatic effects were observed after 24 h and the diameters of clear inhibitory ring were recorded to express antibacterial activity. Results The antibacterial activity of the maggot hemolymph against M.lyso-deiktics and S.aureus was stronger on the plates at pH 6.0 and pH 6.5 than that on the other plates.In the group diluted by pH 6.0 buffer solutions.the antibacterial ring of the maggot hemolymph against M lysodeiktic-us was larger than that in others. Conclusion The results implied that the induced antibacterial substance in Lucilia sericata exhibited stronger antibacterial activity under acidic condition.     

丝光绿蝇抗菌物质在不同pH下抑菌活性的研究

Objective To investigate the antibacterial activity of antibacterial substance in Lucilia seri-cata induced by pricking the skin of maggots(fly s larvae)at different pH conditions. Methods The hemo-lymph from the third instar maggots was collected 48 h after pricking,and the antibacterial effect was tested a-gainst Micrococcus lysodeikticus and Staphylococcus aureus on plates at different pH conditions.The antibacterial effect against M.1ysodeikticus was also detected after the hemolymph、was diluted by different pH buffer solu-tions.The bacteriostatic effects were observed after 24 h and the diameters of clear inhibitory ring were recorded to express antibacterial activity. Results The antibacterial activity of the maggot hemolymph against M.lyso-deiktics and S.aureus was stronger on the plates at pH 6.0 and pH 6.5 than that on the other plates.In the group diluted by pH 6.0 buffer solutions.the antibacterial ring of the maggot hemolymph against M lysodeiktic-us was larger than that in others. Conclusion The results implied that the induced antibacterial substance in Lucilia sericata exhibited stronger antibacterial activity under acidic condition.     

丝光绿蝇抗菌物质在不同pH下抑菌活性的研究

Objective To investigate the antibacterial activity of antibacterial substance in Lucilia seri-cata induced by pricking the skin of maggots(fly s larvae)at different pH conditions. Methods The hemo-lymph from the third instar maggots was collected 48 h after pricking,and the antibacterial effect was tested a-gainst Micrococcus lysodeikticus and Staphylococcus aureus on plates at different pH conditions.The antibacterial effect against M.1ysodeikticus was also detected after the hemolymph、was diluted by different pH buffer solu-tions.The bacteriostatic effects were observed after 24 h and the diameters of clear inhibitory ring were recorded to express antibacterial activity. Results The antibacterial activity of the maggot hemolymph against M.lyso-deiktics and S.aureus was stronger on the plates at pH 6.0 and pH 6.5 than that on the other plates.In the group diluted by pH 6.0 buffer solutions.the antibacterial ring of the maggot hemolymph against M lysodeiktic-us was larger than that in others. Conclusion The results implied that the induced antibacterial substance in Lucilia sericata exhibited stronger antibacterial activity under acidic condition.     

丝光绿蝇抗菌物质在不同pH下抑菌活性的研究

Objective To investigate the antibacterial activity of antibacterial substance in Lucilia seri-cata induced by pricking the skin of maggots(fly s larvae)at different pH conditions. Methods The hemo-lymph from the third instar maggots was collected 48 h after pricking,and the antibacterial effect was tested a-gainst Micrococcus lysodeikticus and Staphylococcus aureus on plates at different pH conditions.The antibacterial effect against M.1ysodeikticus was also detected after the hemolymph、was diluted by different pH buffer solu-tions.The bacteriostatic effects were observed after 24 h and the diameters of clear inhibitory ring were recorded to express antibacterial activity. Results The antibacterial activity of the maggot hemolymph against M.lyso-deiktics and S.aureus was stronger on the plates at pH 6.0 and pH 6.5 than that on the other plates.In the group diluted by pH 6.0 buffer solutions.the antibacterial ring of the maggot hemolymph against M lysodeiktic-us was larger than that in others. Conclusion The results implied that the induced antibacterial substance in Lucilia sericata exhibited stronger antibacterial activity under acidic condition.     

丝光绿蝇抗菌物质在不同pH下抑菌活性的研究

Objective To investigate the antibacterial activity of antibacterial substance in Lucilia seri-cata induced by pricking the skin of maggots(fly s larvae)at different pH conditions. Methods The hemo-lymph from the third instar maggots was collected 48 h after pricking,and the antibacterial effect was tested a-gainst Micrococcus lysodeikticus and Staphylococcus aureus on plates at different pH conditions.The antibacterial effect against M.1ysodeikticus was also detected after the hemolymph、was diluted by different pH buffer solu-tions.The bacteriostatic effects were observed after 24 h and the diameters of clear inhibitory ring were recorded to express antibacterial activity. Results The antibacterial activity of the maggot hemolymph against M.lyso-deiktics and S.aureus was stronger on the plates at pH 6.0 and pH 6.5 than that on the other plates.In the group diluted by pH 6.0 buffer solutions.the antibacterial ring of the maggot hemolymph against M lysodeiktic-us was larger than that in others. Conclusion The results implied that the induced antibacterial substance in Lucilia sericata exhibited stronger antibacterial activity under acidic condition.     

丝光绿蝇抗菌物质在不同pH下抑菌活性的研究

Objective To investigate the antibacterial activity of antibacterial substance in Lucilia seri-cata induced by pricking the skin of maggots(fly s larvae)at different pH conditions. Methods The hemo-lymph from the third instar maggots was collected 48 h after pricking,and the antibacterial effect was tested a-gainst Micrococcus lysodeikticus and Staphylococcus aureus on plates at different pH conditions.The antibacterial effect against M.1ysodeikticus was also detected after the hemolymph、was diluted by different pH buffer solu-tions.The bacteriostatic effects were observed after 24 h and the diameters of clear inhibitory ring were recorded to express antibacterial activity. Results The antibacterial activity of the maggot hemolymph against M.lyso-deiktics and S.aureus was stronger on the plates at pH 6.0 and pH 6.5 than that on the other plates.In the group diluted by pH 6.0 buffer solutions.the antibacterial ring of the maggot hemolymph against M lysodeiktic-us was larger than that in others. Conclusion The results implied that the induced antibacterial substance in Lucilia sericata exhibited stronger antibacterial activity under acidic condition.     

丝光绿蝇抗菌物质的诱导

目的探讨针刺体壁诱导丝光绿蝇产生抗菌物质的规律。方法室内饲养的丝光绿蝇三龄幼虫，经针刺体壁处理后，分别于第0、12、24、36、48、60、72、84h记录幼虫活率，并提取其血淋巴，以金黄葡萄球菌作指示菌，采用平板法，作抑菌试验，37℃恒温24h，观察抑菌圈，并以抑菌圈直径表示抗菌活力的大小，借以指示诱导丝光绿蝇抗菌物质的产生规律。结果经针刺诱导后，丝光绿蝇幼虫活率逐渐降低，血淋巴对金黄葡萄球菌的抑菌活力峰出现在诱导后第48h。结论针刺体壁可诱导丝光绿蝇产生抗菌物质，免疫血淋巴收集在诱导后48h最为合适。     

家蝇抗菌物质诱导及在不同pH下的抑菌活性

目的探讨家蝇幼虫经针刺诱导产生的抗菌物质在不同pH条件下的抑菌活性。方法室内饲养至3龄的家蝇幼虫经针刺诱导48 h后提取血淋巴,平板法作抑菌试验,观察不同pH条件下对溶壁微球菌和金黄色葡萄球菌的抑菌效果。结果针刺诱导产生的抗菌物质对溶壁微球菌的抑菌环直径pH6.5时最大,为（25.17±0.29）mm,随pH继续增高后抑菌环直径减小;对金黄色葡萄球菌的抑菌环直径在pH5.5和6.0时最大,均为（12.83±0.58）mm,pH继续增高后抑菌环直径减小。结论家蝇幼虫经针刺诱导产生的抗菌物质在弱酸性条件下活性较强,随pH增高活性减弱。