Oral l‐glutamine administration attenuated cutaneous wound healing in Wistar rats

The objective of this study was to evaluate the wound healing potential of l ‐glutamine in laboratory rats using excision and incision wound models. Excision wounds of size 500 mm² and depth 2 mm were made on the dorsal portion of male Wistar rats (230–250 g) and were used for the study of oral l ‐glutamine (1 g/kg) treatment on the rate of contraction of wound and epithelisation. Histological evaluation of wound tissue was also performed. Six‐centimetre‐long two linear‐paravertebral incisions in male Wistar rats (230–250 g) were used to study the effect of l ‐glutamine (1 g/kg, p.o.) treatment on tensile strength, total protein and hydroxyproline content in the incision model. Oral administration of l ‐glutamine (1 g/kg) significantly decreased wound area, epithelisation period and wound index, whereas the rate of wound contraction significantly increased (P < 0·001) when compared with vehicle control rats in the excision wound model. Tensile strength, hydroxyproline content and protein level were significantly increased (P < 0·001) in l ‐glutamine (1 g/kg, p.o.)‐treated rats when compared with vehicle control rats in the incision wound model. Histological evaluation of wound tissue from l ‐glutamine (1 g/kg, p.o.)‐treated rats showed complete epithelialisation with new blood vessel formation and high fibrous tissues in the excision wound model. In conclusion, oral administration of l ‐glutamine (1 g/kg) promotes wound healing by acting on various stages of wound healing such as collagen synthesis, wound contraction and epithelialisation.     

两种术式治疗翼状胬肉的效果比较

目的:对比两种手术方法治疗原发性翼状胬肉的临床疗效。方法:原发性翼状胬肉90例(112眼)随机分成两组。A组50例(58眼)行翼状胬肉切除联合带蒂结膜瓣转位术;B组40例(54眼)行翼状胬肉切除联合自体角膜缘干细胞移植。比较两组患者手术时间、角膜创面上皮愈合时间及复发率。结果:A组手术时间为(29.09±2.70)min,明显短于B组(32.72±2.41)min。B组角膜创面上皮愈合时间明显快于A组,组间比较,差异具有统计学意义(P0.05)。术后随诊6~18个月,A组有2眼复发(3.45%),B组有1眼复发(1.85%),差异无统计学意义(P0.05)。结论:两种术式均能有效阻止胬肉复发。但角膜缘干细胞移植术治疗翼状胬肉具有愈合快、并发症少、舒适性高等优点,是目前最值得推广的治疗原发翼状胬肉手术方式。     

重组人表皮生长因子促进兔创面愈合及其量效关系的实验研究

目的：观察重组人表皮生长因子（rh-EGF）对兔皮肤创面愈合的促进作用及其量效关系。方法：制作兔子背部创伤模型,20只兔子背部制作共100个创面,这些创面分为实验组和对照组,实验组包括：低剂量rh-EGF（0.5μg/g）组、中剂量rh-EGF（10μg/g）组、高剂量rh-EGF（100μg/g）组,对照组包括：生理盐水组和EGF基质组,观察五组的大体形态和组织学改变、创面愈合时间和愈合率,测定创面羟脯氨酸（OHP）含量。结果：rh-EGF低剂量组、中剂量组、高剂量组、盐水组和EGF基质组的愈合时间分别为（14.7±0.67）天,（12.9±0.92）天,（11.8±0.99）天,（15.8±0.90）天,（15.5±0.85）天,P〈0.05）。组织学检查可见经rh-EGF高剂量组作用的创面肉芽组织生长活跃,创面的再上皮化迅速。rh-EGF高剂量组的创面中羟脯氨酸含量也最高。结论：rh-EGF对皮肤创面愈合有促进作用,并存在一定的量效关系。以高剂量的rh-EGF（100μg/g）促进作用最大。     

术区持续引流切口愈合后拔管缝合管口治疗脊柱爆裂性骨折术后脑脊液漏疗效观察

目的评价术区持续引流、切口愈合后拔管缝合管口治疗脊柱爆裂性骨折术后脑脊液漏的效果。方法对自2004年6月~2010年6月收治的脊柱爆裂性骨折术后脑脊液漏61例采用体位调节、切口加压包扎治疗(A组,20例),经皮蛛网膜下置管脑脊液引流治疗(B组,20例),术区持续引流、切口愈合后拔管缝合管口治疗(C组,21例)。结果 A组切口脑脊液漏消失时间为(19.0±3.9)d,切口愈合时间为(25.0±4.6)d,8例初期治疗失败。B组切口脑脊液漏的消失时间为(3.0±1.0)d,切口愈合时间为(16.0±2.6)d,6例初期治疗失败。C组切口愈合时间为(13.0±1.0)d,均初期治疗成功。C组切口无脑脊液漏,与A、B组两组相比差异有统计学意义(P<0.05),切口愈合时间短于A、B组两组(P<0.05),初期治疗成功率高于A、B组两组(P<0.05)。结论采用术区持续引流、切口愈合后拔管缝合管口方法治疗脊柱爆裂性骨折术后脑脊液漏疗效满意,效果优于常规方法。     

Effect of Centella asiatica L (Umbelliferae) on normal and dexamethasone-suppressed wound healing in Wistar Albino rats

Centella asiatica is a reputed medicinal plant used in the treatment of various skin diseases in the Indian system of medicine. The objective of the study presented in this article was to evaluate the wound-healing potential of the ethanolic extract of the plant in both normal and dexamethasone-suppressed wound healing. The study was done on Wistar albino rats using incision, excision, and dead space wounds models. The extract of C asiatica significantly increased the wound breaking strength in incision wound model compared to controls (P < .001). The extract-treated wounds were found to epithelize faster, and the rate of wound contraction was significantly increased as compared to control wounds (P < .001). Wet and dry granulation tissue weights, granulation tissue breaking strength, and hydroxyproline content in a dead space wound model also increased at statistically significant levels as shown. The extract of the leaves had the effect of attenuating the known effects of dexamethasone healing in all wound models (P < .001, P < .05). The results indicated that the leaf extract promotes wound healing significantly and is able to overcome the wound-healing suppressing action of dexamethasone in a rat model. These observations were supported by histology findings.     

可吸收胶原蛋白线在口腔种植修复术切口无张力缝合中的应用价值

目的:比较可吸收胶原蛋白线与丝线编织非吸收性缝线在口腔种植修复手术切口无张力缝合中的临床效果及对患者伤口愈合时间的影响。方法:选取2016年8月-2017年8月在笔者医院门诊行牙齿种植修复术的188例患者为研究对象,采用随机数字法将其分为试验组和对照组,每组94例。试验组采用可吸收胶原蛋白线缝合伤口,对照组采用丝线编织非吸收性缝线缝合伤口。比较两组患者伤口愈合等级、平整率、缝合线完全吸收率、切口愈合时间、疼痛情况及不良反应发生情况。结果:试验组:甲级愈合89例,乙级愈合5例;对照组:甲级愈合74例,乙级愈合19例,丙级愈合1例,试验组患者伤口愈合等级显著优于对照组,差异有统计学意义(P<0.05)。试验组患者牙龈红肿5例,对照组患者牙龈红肿16例,渗血10例,试验组患者牙龈红肿、渗血及总发生情况均显著优于对照组,差异有统计学意义(P<0.05)。术后3、5、7d,试验组患者缝线位点完全吸收率分别为0.00%、11.35%、97.52%,对照组患者缝线位点完全吸收率均为0.00%,试验组患者缝线位点完全吸收率在术后3、5d均显著高于对照组,差异均具有统计学意义(P<0.05)。试验组患者切口平整率为85.11%,对照组患者切口平整率为68.09%,差异具有统计学意义(P<0.05)。试验组患者伤口愈合时间为(5.23±0.31)d,对照组患者伤口愈合时间为(7.57±0.36)d,试验组患者伤口愈合时间显著优于对照组,差异有统计学意义(P<0.05)。术后3、5、7d,试验组患者VAS评分分别为(3.17±1.65)分、(2.04±0.88)分、(1.11±0.56)分,对照组患者VAS评分分别为(4.97±1.74)分、(3.74±1.25)分、(2.73±0.93)分,在各个时间节点,试验组患者VAS评分水平均显著优于对照组,差异有统计学意义(P<0.05)。结论:可吸收胶原蛋白线在口腔种植修复手术切口无张力缝合中的临床效果较丝线编织非吸收性缝线的临床效果更加显著,应用可吸收胶原蛋白缝线能够有效提高患者伤口愈合效果、缩短愈合时间,同时减少不良反应,值得在临床推广应用。     

Preliminary investigation of topical nitroglycerin formulations containing natural wound healing agent in diabetes‐induced foot ulcer

Nitroglycerin (NTG) is an organic nitrate rapidly denitrated by enzymes to release free radical nitric oxide and shows improved wound healing and tissue protection from oxidative damage. The purpose of this study was to evaluate whether topical application of NTG in the form of gel/ointment along with a natural wound healing agent, aloe vera, would bring about wound healing by using diabetes‐induced foot ulcer model and rat excision wound model. All these formulations were evaluated for pH, viscosity, drug content and ex vivo diffusion studies using rat skin. Based on ex vivo permeation studies, the formulation consisting of carbopol 974p as a gelling agent and aloe vera was found to be suitable. The in vivo study used streptozotocin‐induced diabetic foot ulcer and rat excision wound models to analyse wound healing activity. The wound size in animals of all treated groups was significantly reduced compared with that of the diabetic control and marketed treated animals. This study showed that the gel formed with carbopol 974p (1%) and aloe vera promotes significant wound healing and closure in diabetic rats compared with the commercial product and provides a promising product to be used in diabetes‐induced foot ulcer.     

Local gentamicin application for perineal wound healing following abdominoperineal rectum excision

A major complication of abdominoperineal rectum excision for rectal or anal carcinoma is local wound infection. The main reason for this infection is that systemically administered antibiotic prophylaxis does not reach sufficiently high concentrations of antibiotic in the tissue of the sacroperineal wound. Since gentamicin-polymethylmethacrylate (PMMA) in the form of chains of beads has been confirmed as a useful tool in the treatment of local infection in bone and soft tissue surgery, we have evaluated its effect on the abdominoperineal rectum excision in a prospective, randomized trial. Forty-four patients with rectal or anal carcinoma entered the study. Only patients with stage Dukes D were excluded from the trial. Following randomized selection, the patients were treated either with local gentamicin and drainage (Group A) or drainage alone (Group B), using the Lloyd-Davies procedure. The two groups were comparable regarding age, sex, tumor stage and level, and risk factors predisposing for an infectious complication (anemia, nutritional status, blood transfusion). The postoperative mortality rate was 0% in both series. Analysis of local perineal wound healing revealed a statistically significant higher percentage of primary wound healing in Group A than in Group B (87% versus 46%; p less than 0.01). This led to a significantly shorter hospitalization period for patients in Group A (p less than 0.01). Gentamicin-PMMA chains have been demonstrated to exert a favorable effect on local wound healing and the postoperative outcome of patients with abdominoperineal rectum excision.     

Safety evaluation of topically applied amitriptyline in porcine full-thickness wounds

BACKGROUND AND OBJECTIVES: The tricyclic antidepressant amitriptyline is frequently used in pain clinics for management of pain. It has also been suggested that topical application of amitriptyline could be useful for the treatment of neuropathic pain. In this report we investigated the effect of amitriptyline on porcine full thickness wounds resembling excised burn wounds. We assessed if daily topical application of amitriptyline into the wound chambers for 10 days impedes wound healing as measured by (1) wound contraction and (2) histopathological findings. METHODS: Full-thickness wounds measuring 1.5 cm square were created on the dorsum of Yorkshire pigs and were enclosed in polyurethane wound chambers. Amitriptyline was applied daily at various concentrations. Bupivacaine (0.5%) or normal saline were used as controls. Daily wound serum levels were obtained and the level of amitriptyline and nortriptyline obtained. Pictures were taken daily and the wound surface analyzed for contraction. Cross-sectional, full-thickness skin biopsies were obtained at days 2, 8 and 10 and evaluated microscopically for re-epithelialization, inflammation, and necrosis. RESULTS: The high serum level of amitriptyline and nortriptyline did not affect wound healing; re-epithelialization, wound contraction, and inflammation were not significantly different between amitriptyline and control groups. CONCLUSION: Amitriptyline at the concentrations of 0.0625% and 0.125% applied daily via chambers covering wounds in a full-thickness pig excision model has no overt toxic effect on wound healing as measured by wound contraction and histological assessment.     

藏毛窦切除术后切口开放、缝合或皮瓣转移的疗效回顾

目的探讨藏毛窦手术3种不同切口处理方法的临床效果。方法 2005年5月～2009年10月施行藏毛窦手术62例,随访43例,其中单纯切除术12例(切口开放组),切除后缝合18例(切口缝合组),切除后行各种皮瓣转移术13例(皮瓣转移组,包括菱形皮瓣转移6例,椭圆形皮瓣转移5例,三角形皮瓣转移2例)。结果单纯切除组切口愈合时间(70.1±11.6)d,术后第1天疼痛VAS评分(3.7±0.9)分,愈合后切口不适、针刺样疼痛7例,其中2例瘢痕处反复脱皮,无复发。切口缝合组切口愈合时间(34.3±7.6)d,术后第1天疼痛VAS评分(3.7±1.1)分,术后切口渗出10例,裂开5例,皮肤及皮下组织坏死1例,切口不适、针刺样疼痛3例,无复发。皮瓣转移组切口愈合时间(21.2±6.1)d,术后第1天疼痛VAS评分(2.3±0.9)分,切口裂开2例,前8例中2例皮肤表皮层坏死,改善缝合方法后5例无皮肤坏死,切口不适1例,1例复发。结论藏毛窦手术后切口闭合愈合时间较短,不影响手术效果,择期行切口闭合手术应是较好选择。闭合方法的选择根据切口张力的大小而定,张力小时可直接缝合切口,张力大时采用皮瓣转移术。     

腹腔镜中低位直肠癌全直肠系膜切除术的临床分析

目的探讨腹腔镜全直肠系膜切除术治疗中低位直肠癌的近-中期疗效,评价其在治疗中低位直肠癌上的优势。方法回顾性对比分析2010年1月至2014年12月收治的318例中、低位直肠癌患者的临床资料,按手术方法分为腹腔镜组218例,开腹组100例,采用SPSS 17.0软件进行统计分析,比较两组患者手术情况、术后恢复情况、标本肿瘤学指标,以上计量资料用均数±标准差表示,组间比较采用t检验。两组患者复发率或转移率比较采用χ2检验或Fisher确切概率法,P0.05为差异有统计学意义。结果两组患者术中出血量、切口长度、保肛率、术后疼痛VSA评分、术后胃肠道功能恢复时间、切口愈合时间、术后住院时间及围手术期并发症发生率等指标腹腔镜组明显优于开腹组,差异有统计学意义(P0.05);而在肿瘤下缘距远切缘距离、切缘阳性率、淋巴结清扫数目、手术时间等指标上差异无统计学意义。总并发症发生率:腹腔镜组为3.2%(7/218),开腹组为18.0%(18/100),差异有统计学意义(χ2=5.032,P0.05)。结论腹腔镜全直肠系膜切除治疗中、低位直肠癌是安全、可行的,其相较传统开腹手术存在明显的微创优势,值得临床广泛应用。     

两种手术方法治疗骶尾部藏毛窦 43例的临床分析

目的:评价切开引流囊壁搔刮术与切除袋形缝合术治疗骶尾部藏毛窦的疗效。方法:回顾性分析43例骶尾部藏毛窦患者的临床资料,其中行切开引流囊壁搔刮术治疗(切口开放搔刮组)20例,行切除袋形缝合术治疗(切除袋形缝合组)23例。结果:切口开放搔刮组、切除袋形缝合组的手术时间分别为(30.40±5.22)min、(43.49±7.45)min,住院时间分别为(7.70±1.42)d、(8.17±1.47)d,愈合时间分别为(34.45±7.72)d、(24.48±5.96)d,术后疼痛NRS评分分别为(4.15±1.09)、(4.74±0.96)。两组手术时间、切口愈合时间的差异具有统计学意义(P<0.05),住院时间、术后疼痛的差异无统计学意义(P>0.05)。随访1~6年,两组均无复发。结论:彻底切除病变组织是治愈骶尾部藏毛窦的关键,急性感染期选择切开引流囊壁搔刮术、慢性窦道期选择切除袋形缝合术均可治愈骶尾部藏毛窦。     

Acceleration of wound repair by curcumin in the excision wound of mice exposed to different doses of fractionated γ radiation

Fractionated irradiation (IR) before or after surgery of malignant tumours causes a high frequency of wound healing complications. Our aim was to investigate the effect of curcumin (CUM) on the healing of deep excision wound of mice exposed to fractionated IR by mimicking clinical conditions. A full-thickness dermal excision wound was created on the shaved dorsum of mice that were orally administered or not with 100 mg of CUM per kilogram body weight before partial body exposure to 10, 20 or 40 Gy given as 2 Gy/day for 5, 10 or 20 days. The wound contraction was determined periodically by capturing video images of the wound from day 1 until complete healing of wounds. Fractionated IR caused a dose-dependent delay in the wound contraction and prolonged wound healing time, whereas CUM administration before fractionated IR caused a significant elevation in the wound contraction and reduced mean wound healing time. Fractionated IR reduced the synthesis of collagen, deoxyribonucleic acid (DNA) and nitric oxide (NO) at different post-IR times and treatment of mice with CUM before IR elevated the synthesis of collagen, DNA and NO significantly. Histological examination showed a reduction in the collagen deposition, fibroblast and vascular densities after fractionated IR, whereas CUM pre-treatment inhibited this decline significantly. Our study shows that CUM pre-treatment accelerated healing of irradiated wound and could be a substantial therapeutic strategy in the management of irradiated wounds.     

The effect of epigallocatechin-3-gallate, a constituent of green tea, on transforming growth factor-β1–stimulated wound contraction

Dermal fibrosis, or scarring, following surgical incisions, traumatic wounds and burns presents a major clinical burden. Transforming growth factor (TGF)-β1 is a major factor known to stimulate fibroblast proliferation, collagen production, and the differentiation of fibroblast to myofibroblast promoting wound contraction. Furthermore, excessive or prolonged TGF-β1 has been shown to be associated with scarring. Green tea contains high amounts of polyphenols with the major polyphenolic compound being epigallocatechin-3-gallate (EGCG). EGCG has been shown to be anti-inflammatory, anti-oxidant, and may improve wound healing and scarring, though its precise effect on TGF-β1 remains unclear. This study aimed at determining the effect of EGCG on TGF-β1 collagen contraction, gene expression and the differentiation of fibroblast to myofibroblast. EGCG appears to affect the role that TGF-β1 plays in fibroblast populated collagen gel contraction and this seems to be through both myofibroblast differentiation and connective tissue growth factor gene expression and reduces the expression of collagen type I gene regulation.     

次侧方内括约肌切开加双缘结扎术治疗Ⅲ期肛裂临床观察(附150例报告)

目的观察次侧方内括约肌切开加双缘结扎术治疗Ⅲ期肛裂临床疗效及其对肛门功能的影响。方法治疗组选取Ⅲ期肛裂患者150例,均采用次侧方内括约肌切开加双缘结扎术治疗,观察创口愈合时间、随访6个月的复发率及测定手术前后肛门直肠压力,并与健康对照组比较。结果所有患者均痊愈出院,创口平均愈合时间(16±2.87)d,随访6个月无复发病例;术前肛管静息压较正常人显著升高(P0.05),肛管舒张压较前显著降低(P0.05),肛管最大收缩压及肛管自主收缩持续时间与正常人比较无明显差异(P0.05);术后肛管静息压较术前明显降低(P0.05),肛管舒张压较前明显升高(P0.05),肛管最大收缩压及肛管自主收缩持续时间与术前比较无明显差异(P0.05);术后肛管静息压、肛管舒张压、肛管最大收缩压及肛管自主收缩持续时间与正常人比较无明显差异(P0.05)。结论次侧方内括约肌切开加双缘结扎术治疗Ⅲ期肛裂疗效显著,能有效地解除内括约肌痉挛,改善肛裂溃疡面的供血不足,促进肛裂的愈合。     

脱细胞真皮基质在整形美容外科中的应用

目的 为改善皮肤移植的形态和功能，提高大面积烧伤缺乏自体供皮来源患者的美容整形治疗效果。方法 应用自制脱细胞真皮基质(ADM)，植入松解或切除瘢痕后的创面，5～10d后再行自体刃厚皮移植，术后观察ADM和创面愈合、瘢痕增生及功能恢复情况。3例再次手术患者留取复合移植组织进行组织学检测。结果 15例23次复合皮移植：14例22次成活率为100％，1例1次成活率为80％，植皮区平整光滑，瘢痕增生不明显，组织学检测提示复合皮血管化明显，胶原排列整齐，表皮-真皮连接结构重建充分，未见排异反应。结论 ADM与自体刃厚皮复合移植能改善创面愈合的质量，是较理想的整形美容修复材料。     

几种深Ⅱ度烧伤创面处理方法的回顾及改善创面微循环的初步实验研究

目的回顾性分析几种深Ⅱ度烧伤创面的修复方法,探讨改善创面微循环对创面愈合的意义. 方法 (1)对于笔者单位烧伤患者的深Ⅱ度创面,应用削痂疗法治疗614例、磨痂疗法治疗32例、清创后异体皮覆盖86例、外用磺胺嘧啶银后创面暴露1 836例、外用中药京万红烫伤膏包扎治疗408例.统计、分析各种疗法的治疗效果.(2)制作大鼠深Ⅱ度烫伤模型.伤后5 min内分别由其尾静脉注入等渗盐水(对照组,10只)、巴曲酶(治疗组,10只),创面均外用磺胺嘧啶银.测定两组大鼠伤前及伤后0.5-72.0 h的创面皮肤血流灌注单位,计算其伤后14、18 d的创面愈合率、收缩率及创面愈合时间.用组织学方法观察两组大鼠创面愈合后的皮肤毛囊数. 结果 (1)削痂疗法术后2-3周创面愈合,其中烧伤总面积50%～79%TBSA的患者治愈率94.8%,总面积80%～98%TBSA者治愈率93.4%.磨痂疗法磨痂+异体皮覆盖术后(13.8±2.1)d创面愈合,无瘢痕形成.清创后异体皮覆盖其中82例患者术后(18.0±2.3)d创面愈合.外用磺胺嘧啶银后暴露其中1 658例患者用药后(26.0±3.2)d痂下愈合.外用京万红烫伤膏后包扎患者多有细菌感染,其中下肢创面愈合时间为(26.0±2.8)d.(2)治疗组大鼠伤后2.0-72.0 h创面局部血流灌注单位均明显高于对照组(P<0.01).伤后14、18 d,治疗组创面愈合率明显高于对照组(P<0.01),但两组创面收缩率接近(P>0.05).治疗组创面愈合时间短于对照组(P<0.01).伤后30 d,对照组大鼠真皮层中残存少量毛囊,数量明显少于治疗组(P<0.01). 结论深Ⅱ度烧伤后早期采用削痂、磨痂或清创后覆盖异体皮的方法处理创面,可减轻感染、缩短疗程、提高治愈率和愈合质量.使用巴曲酶可改善深Ⅱ度烧伤创面微循环,加快愈合速度.     

不同植骨材料对跟骨骨折切口愈合的影响

目的研究两种不同植骨材料（白体髂骨、同种异体骨）对跟骨骨折术后切口愈合的影响。方法回顾性研究安徽医科大学第一附属医院自2009年1月至2012年12月采用切开复位AO钢板内固定加植骨术治疗的76例单侧跟骨骨折患者,其中男53例,女23例,平均年龄39．5岁。自体髂骨植骨40例（A组）,同种异体骨植骨36例（B组）。应用统计学方法对患者年龄、伤后至手术时间、切口干燥时间、切口愈合时间、术后24h切口引流量等进行评价。结果A组平均年龄（39．43±11．71）岁,伤后至手术平均时间（10．27±2．54）d,切口平均干燥时间（6．02±1．37）d,切口平均愈合时间（14．08±1．27）d,术后24h平均引流量（78．58±13．92）mL;B组平均年龄（39．53±110．52）岁,伤后至手术平均时间（9．14±3．19）d,切口平均干燥时间（8．08±1．02）d,切口平均愈合时间（16．14±1．84）d,术后24h平均负压引流量（96．69±13．57）mL。A组与B组的切口干燥时间、愈合时间、术后24h引流量的比较差异有统计学意义（P〈0．05）。结论自体髂骨植骨较同种异体骨植骨会缩短跟骨骨折切口的愈合时间。     

Effect of low level laser therapy on wound healing after palatal surgery in beagle dogs.

The effect of low level laser therapy on wound healing and wound contraction after palatal surgery in Beagle dogs of 12 weeks of age was investigated. A total of 30 Beagle dogs was used and assigned to a control group (Group C; n = 6) and two experimental groups (Group L; n = 12 and group LL; n = 12). In both experimental groups, Von Langenbeck's palatal repair was simulated. Then in the LL group the denuded bony areas were treated with low level laser therapy using a continuous Ga-As-A1 laser beam (830 nm) and energy output set at 30 mW. Per treatment a dosage of 1 J/cm2 wound surface area was used. Therapy was carried out three times a week with a total of ten treatments. Wound healing was observed clinically until wound healing was completed at 4 weeks p.o. and wound areas were measured at regular intervals on standardized intra-oral photographs. Wound contraction was measured as the increments of the distances between tattoo points on the opposite wound margins. No significant differences were found in the quality and rate of wound healing between the two experimental groups. The same held true for the increments of the distances between opposite tattoo points. It was concluded that macroscopically low level laser therapy under conditions used in this study did not have an influence on wound closure or wound contraction.     

解毒生肌汤和紫草油纱在肛瘘术后的应用

目的:观察解毒生肌汤溻渍配合紫草油纱换药促进肛瘘多切口浮线引流术后创面愈合的疗效。方法:将112例接受肛瘘多切口浮线引流手术患者随机分为对照组与观察组各56例,两组均予常规治疗。对照组于便后采用温水溻渍并紫草油纱条塞入,观察组采用解毒生肌汤溻渍联合紫草油纱辅助引流,观察两组第1、4、9、14天和1、2个月的肛瘘创面分泌物渗出及伤口水肿、疼痛程度、创面愈合率及愈合时间等。结果:术后第4、9、14天,观察组创面分泌物评分分别为(3.74±0.42)分、(3.55±0.51)分、(3.11±0.37)分,高于对照组的(3.41±0.62)分、(3.23±0.54)分、(2.74±0.47)分,差异有统计学意义(P<0.05)。观察组创面水肿评分(1.26±0.26)分,明显低于对照组的(2.74±0.48)分。观察组术后第9、14天和1、2个月创面愈合率分别为(15.17±3.48)%、(31.28±2.74)%、(65.18±7.15)%、(85.21±6.18)%,高于对照组的(9.31±2.15)%、(19.37±2.71)%、(45.27±6.18)%、(79.38±5.48)%,差异有统计学意义(P<0.05)。对照组创面愈合时间为(46.37±5.27)d,明显高于观察组的(57.24±8.12)d,观察组术后第4、9、14天和1个月疼痛VAS评分分别为(7.24±1.02)分、(5.94±0.84)分、(3.75±0.49)分、(2.57±0.31)分,低于对照组的(8.01±1.28)分、(6.27±0.87)分、(4.31±0.47)分、(3.15±0.25)分,差异均有统计学意义(P<0.05)。结论:解毒生肌汤溻渍联合紫草油纱条换药可显著增加肛瘘多切口浮线引流术后前期良性渗出,减轻创面水肿,降低患者术后疼痛程度,加快创面愈合。