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P-糖蛋白、bcl-2蛋白和增殖细胞核抗原在肺癌组织中的表达及意义 总被引:1,自引:0,他引:1
目的:研究P-糖蛋白(P-glycoprotein,P-gp)的表达与bcl-2蛋白和增殖细胞核抗原(proloferating-cellnuclear antigen,PCNA)表达的关系及意义。方法:用特异性鼠抗人单克隆抗体,用SP免疫组织化学方法检测石蜡包埋的肺癌标本中P-gp,bcl-2蛋白和PCNA的表达,结果:在小细胞肺癌(samll cell lung cancer,SCLC)中,P 相似文献
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目的:探讨鼻咽癌(NPC)组织bcl-2及EB病毒潜伏膜蛋白(LMP)表达与放射诱发细胞凋亡的关系。方法采用免疫组化S-P法及TdT酶介导的生物素化dUTP缺口要端标记技术(TUNEL法),分别检测35例NPC组中bcl-2及EB病毒LMP的表达,以及放疗总量为10Gy时NPC组织的细胞凋亡率(AR)。结果NPC组织bcl-2及LMP的表达率分别为71.4%(25/35)、45.7%(16/35) 相似文献
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目的:探讨常规分割放疗和超分割放疗对鼻咽癌组织细胞增殖凋亡的影响。方法:采用TdT酶介导的生物素化dutp缺口末端标记技术(TUNEL)和免疫组织化学S-P法,分别检测60例鼻咽癌患者在放疗第2周和第4周时细胞凋亡率(apoptosis rate,AR)和增殖细胞核抗原(proliferation cell nhuclear antigen,PCNA)的表达。结果:常规分割放疗组放疗第4周后的AR 相似文献
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目的 研究消化管类癌中分化抗原(chromograninA),浸润转移相关抗原urokinase,培殖细胞核抗原(PCNA)及细胞凋亡相关抗原BM-1的表达与该肿瘤恶性程度的关系。方法 应用免疫组化技术(SAB法)检测随访8年以上的13例(其中无转移9例,其他器官转移4例)消化管类癌肿瘤组织chromograninA,urckinase,PC-NA及BM-1的表达状况,并分析这些基因产物表达与该肿 相似文献
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TSP、VEGF与大肠癌血管生成、转移关系的研究 总被引:6,自引:0,他引:6
目的 分析小板反应素(thrombospondin,TSP)、血管内皮生长因子(vascular endothelial growth factor,VEGF)表达和大肠癌血管生成、远处转移之间的关系。方法 对47例大肠癌手术标本采用逆转录多聚酶链反应(RT-PCR)检测TSP1和TSP2mRNA表达,并采用免疫组化法检测微血管计数(microvessel count,MVC)和VEGF蛋白表达。结果 大肠癌MVC和VEGF表达的程度呈正相关(r=0.438,P=0.002),在淋巴结转移和远处转移者高于无转移者(P〈0.05)。TSP2mRNA表害和MVC(r=-0.362,P=0.01)、VEGF表达(r=-0.322,P〈0.05)有明显的负相关。TSP2mRNA表达率在远处转移病人中低于没有远处转移者( 相似文献
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肾盂输尿管癌中p53基因突变及增殖细胞核抗原表达的临床意义 总被引:3,自引:0,他引:3
为探讨肾盂输尿管癌中p53基因突变及增殖细胞核抗原表达的临床意义,采用免疫组化S-P法,对45例肾盂输尿管癌突变型P53和增殖细胞核坑原表达进行研究。结果表明:突变型P53阳性染占53.3%,TE+54突变型P53阳性表达,PCNA染色分级高于T1+T2;G3突变型P53阳性表达,PCNA染色分级高于G1+G2。 相似文献
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乳腺癌组织中金属硫蛋白过表达生物学意义的探讨 总被引:1,自引:0,他引:1
为了弄清MTs在乳腺癌过表达的生物学意义,我们应用免疫组化LSAB法对107例乳腺癌进行了检测。结果:86例(80.4%)乳腺癌有MTs过表达,阳性物质位于癌细胞核内,或者同时位于细胞浆内。MTs在浸润性乳腺癌中的表达与病理分级关系密切,Ⅱ级以上者阳性率明显高于Ⅰ级(P<0.05);肿瘤最大直径小于2.5cm者MTs表达率明显少于2.5cm以上者(P<0.05);核分裂多者MTs表达率明显高于核分裂少者(P<0.01)。c-erbB-2、p53、bc1-2癌蛋白和nm23蛋白表达情况与MTs过表达之间未见明显相关性。PCNA与MTs过表达有关,PCNA指数在50%以上者的MTs表达率明显高于PCNA指数在50%以下者(P<0.05)。有MTs过表达的患者生存期明显短于无MTs过表达者(P<0.05)。表明MTs表达可作为预示乳腺癌进展快和预后差的生物学标志之一。 相似文献
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增殖细胞核抗原,胎盘型谷胱甘肽S—转移酶在肝细胞癌及癌周组织中的… 总被引:2,自引:0,他引:2
目的 探讨增生结节与肝细胞癌发生的关系,方法 应用免疫组织化学方法,分别检测70例肝细胞癌及癌周组织中增殖细胞核抗原(PCNA)的表达,以及77例肝细胞癌和癌周组织中胎盘型谷胱甘肽S-转移酶(GSTπ)的表达。结果 PCNA和GSTπ在肝细胞癌中的阳性表达率显著高于癌周组织,且PCNA与肝细胞癌Edmondson分级密切相关;癌周组织中,增生结节PCNA和GSTπ阳性表达显著高于肝硬变及正常组织, 相似文献
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Information on occupation, smoking, food and beverage consumption, and medical history were compared between 275 incident cases of carcinoma of the esophagus and 275 neighborhood controls who were matched to the cases on age (within 5 years), race, and sex. Tobacco use, mainly cigarette smoking, was a significant risk factor for carcinoma of the esophagus. Ex-smokers of cigarettes showed a reduced risk relative to those who continued to smoke, and current smokers of two or more packs per day displayed a higher risk than those who smoked less. Alcohol consumption was another significant risk factor for carcinoma of the esophagus; there was a highly significant trend with average daily dose of ethanol. Relative to controls, cases also consumed significantly more fried bacon or ham, less fresh fruits and raw vegetables, and were more likely to prefer white than whole grain bread. Finally, there was a significant association between carcinoma of the esophagus and long-term occupational exposure to metal dust; this association was largely confined to the lower one-third section of the esophagus. 相似文献
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Lynn D. Wilson Ginette A. Hinds James B. Yu 《Clinical Lymphoma, Myeloma & Leukemia》2012,12(5):291-296
BackgroundThe incidence of the T- and B-cell CLs has been well documented, but information pertaining to racial incidence by age, and by burden of disease (stage) have not been extensively documented.Materials and MethodsThe SEER 2004-2008 public use database was investigated. The relative incidence of CL in different races and age groups was examined. Univariate and multivariate stepwise logistic regression was performed for the likelihood of presenting at a higher stage.ResultsOf 4496 patients diagnosed with CL between 2004 and 2008; 1713 patients were diagnosed with MF, 1518 with non-MF cutaneous T-cell lymphoma, and 1265 patients with cutaneous B-cell lymphoma. For MF, there was a trend for females to be less likely to present with a higher T-stage (T3-T4) than males (odds ratio [OR], 0.73) on multivariate analysis (P = .06). For race, AA had a significantly increased risk of presenting with higher T-stage (T3-T4) MF (OR, 1.72) on multivariate analysis (P = .02), compared with white patients. For white, AA, Asian/Pacific Islander, and Native American/other/unknown, the mean age at diagnosis was 59.2, 51.5, 51.3, and 53.8. These groups presented at a significantly different age than white (P = .0001, 0.0001, and 0.0006).ConclusionNonwhite racial groups present with MF at an earlier age compared with white, and AA have increased risk of presenting with higher T-stage compared with white. These findings have significant implications regarding need for earlier diagnosis and understanding the reasons for racial disparity in age and stage of presentation. 相似文献
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Fat, fiber, fruits, vegetables, and risk of colorectal adenomas 总被引:5,自引:0,他引:5
Mathew A Peters U Chatterjee N Kulldorff M Sinha R 《International journal of cancer. Journal international du cancer》2004,108(2):287-292
A case-control study was conducted at the National Naval Medical Center (Maryland, USA) from 1994 to 1996 to investigate the possible association between dietary factors and colorectal adenomas. Cases (n = 239) were subjects diagnosed with adenomas (146 new and 93 recurrent) by sigmoidoscopy or colonoscopy. Those with no evidence of adenomas found by sigmoidoscopy were recruited as controls (n = 228). Dietary variables, assessed by a 100-item food frequency questionnaire, were analyzed by the logistic regression model, which was adjusted for age, gender and total energy intake. Variables of fat intake were further adjusted for red meat intake. An increased risk of 7% [odds ratio (OR): 1.07; 95% confidence interval (95% CI): 0.94-1.22] per 5% energy/day from total fat was observed. Every additional 5% unit of oleic acid intake/day significantly increased the adenoma risk by 115% (OR: 2.15; 95% CI: 1.05-4.39). Red meat fat increased the risk by 20% (OR: 1.20; 95% CI: 0.71-2.04), and white meat fat decreased the risk by 67% (OR: 0.33; 95% CI: 0.19-0.95) for every additional 5% unit of respective intake/day. Risk decreased by 41% (OR: 0.59; 95% CI: 0.41-0.86) for every additional 5% unit of fiber intake/day. Vegetable [OR per 100 g of vegetable intake/day: 0.83, 95% CI: 0.67-1.04] and fruit (OR per 100 g of fruit intake/day: 0.92, 95% CI: 0.82-1.03) intake showed an inverse association, and the results are suggestive of an association with the risk for adenomas. In conclusion, a strong positive association between oleic acid intake and colorectal adenoma risk was observed. This is likely to be an indicator of "unhealthy" food (meat, dairy, margarine, mayonnaise, sweet baked food) consumption in this population. Increased intake of dietary fiber was associated with a moderately decreased risk of adenomas. 相似文献
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《Journal of chemotherapy (Florence, Italy)》2013,25(2):77-79
AbstractThe in vitro activity of tetracycline, doxycycline, erythromycin, roxithromycin, clarithromycin, azithromycin, levofloxacin and moxifloxacin was tested against 63 clinical isolates of Ureaplasma urealyticum. The minimal inhibitory concentrations (MICs) and the minimal bactericidal concentrations (MBCs) were determined by the broth microdilution method in A7 medium. The miC50 and miC90 of the tested agents after 24 h of incubation were as follows: Tetracycline, 0.5 and 2.0 μg/ml; doxycycline, 0.125 and 0.25 μg/ml; erythromycin, 2.0 and 8.0 μg/ml; roxithromycin, 2.0 and 4.0 μg/ml; clarithromycin, 0.25 and 1.0 μg/ml; azithromycin, 2.0 and 4.0 μg/ml; levofloxacin, 1.0 and 2.0 μg/ml; and moxifloxacin, 0.5 and 0.5 μg/ml, respectively. The MIC values after 24 h and 48 h incubation differed by no more than one dilution for all the agents with the exception of doxycycline (two dilution difference for MIC90). Overall, moxifloxacin was the most active agent in vitro against U. Urealyticum, with the narrowest difference between MIC and MBC values, followed closely by levofloxacin. Clarithromycin was the most active macrolide. 相似文献
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The in vitro activity of tetracycline, doxycycline, erythromycin, roxithromycin, clarithromycin, azithromycin, levofloxacin and moxifloxacin was tested against 63 clinical isolates of Ureaplasma urealyticum. The minimal inhibitory concentrations (MICs) and the minimal bactericidal concentrations (MBCs) were determined by the broth microdilution method in A7 medium. The MIC(50) and MIC(90) of the tested agents after 24 h of incubation were as follows: tetracycline, 0.5 and 2.0 μg/ml; doxycycline, 0.125 and 0.25 μg/ml; erythromycin, 2.0 and 8.0 μg/ml; roxithromycin, 2.0 and 4.0 μg/ml; clarithromycin, 0.25 and 1.0 μg/ml; azithromycin, 2.0 and 4.0 μg/ml; levofloxacin, 1.0 and 2.0 μg/ml; and moxifloxacin, 0.5 and 0.5 μg/ml, respectively. The MIC values after 24 h and 48 h incubation differed by no more than one dilution for all the agents with the exception of doxycycline (two dilution difference for MIC(90)). Overall, moxifloxacin was the most active agent in vitro against U. urealyticum, with the narrowest difference between MIC and MBC values, followed closely by levofloxacin. Clarithromycin was the most active macrolide. 相似文献
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JIA-LONG Fang ROBIN L. STINGLEY FREDERICK A. BELAND WAFA HARROUK DEBBIE L. LUMPKINS PAUL HOWARD 《journal of environmental science and health part c-environmental carcinogenesis & ecotoxicology reviews》2013,31(3):147-171
Triclosan has broad-spectrum anti-microbial activity against most gram-negative and gram-positive bacteria. It is widely used in personal care products, household items, medical devices, and clinical settings. Due to its extensive use, there is potential for humans in all age groups to receive life-time exposures to triclosan, and, indeed, triclosan has been detected in human tissues and the environment. Data gaps exist regarding the chronic dermal toxicity and carcinogenicity of triclosan, which is needed for the risk assessment of triclosan. The US Food and Drug Administration (FDA) nominated triclosan to the National Toxicology Program (NTP) for toxicological evaluations. Currently, the NTP is conducting several dermal toxicological studies to determine the carcinogenic potential of triclosan, evaluate its endocrine and developmental-reproductive effects, and investigate the potential UV-induced dermal formation of chlorinated phenols and dioxins of triclosan. This paper reviews data on the human exposure, environmental fate, efficacy of anti-microbial activity, absorption, distribution, metabolism and elimination, endocrine disrupting effects, and toxicity of triclosan. 相似文献
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Vincent Grégoire Mererid Evans Quynh-Thu Le Jean Bourhis Volker Budach Amy Chen Abraham Eisbruch Mei Feng Jordi Giralt Tejpal Gupta Marc Hamoir Juliana K. Helito Chaosu Hu Keith Hunter Jorgen Johansen Johannes Kaanders Sarbani Ghosh Laskar Anne Lee Cai Grau 《Radiotherapy and oncology》2018,126(1):3-24
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Alan S. Morrison Karl H. Proppe Wendy G. Verhoek Kunio Aoki Ian Leck Yoshiyuki Ohno Koji Obata 《International journal of cancer. Journal international du cancer》1982,30(6):701-705
Histologic characteristics of bladder cancer in Boston, USA, Manchester, UK, and Nagoya, Japan, were evaluated. In each of these areas broadly-based series of cases were assembled during a collaborative case-control study. The present analysis was based on 589 cases in Boston, 484 cases in Manchester, and 241 cases in Nagoya. A single pathologist reviewed a slide of the primary tumor without reference to identifying information or other data. The primary histologic type of nearly all tumors was transitional-cell, and there was little variation in the proportion of transitional-cell tumors among the study areas. Nor was there much variation in the distribution of histologic grade, the proportion of tumors showing submucosal invasion, or the proportion of tumors with a papillary surface. Age at diagnosis was strongly correlated with histologic grade. The proportion of grade III (most malignant) tumors was about twice as high among patients 80 years of age and over as among those aged less than 50. An apparent association between age and submucosal invasion was explained in large part by the relationships of histologic grade to submucosal invasion and to age. Other histologic features had only weak and inconsistent relations with age. None of the features evaluated showed consistent associations with history of cigarettesmoking or with sex. 相似文献
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Scott D. Ramsey Ingrid J. Hall Judith Lee Smith Donatus U. Ekwueme Catherine R. Fedorenko Karma Kreizenbeck Aasthaa Bansal Ian M. Thompson David F. Penson 《Journal of Geriatric Oncology》2021,12(2):305-311
ObjectivesStudies of local stage prostate cancer survivors suggest that treatments carry risk of persistent impotence, incontinence, and bowel dysfunction. To examine impacts of cancer type and side effects on health-related quality of life (HRQoL) in long-term cancer survivorship, we evaluated 5-year follow-up of patients with prostate cancer and compared results with a matched group of male long-term survivors of other local-stage cancers.Materials and MethodsWe examined genitourinary, bowel and sexual symptoms, and general quality of life. Matched survivors of colorectal, lung, and bladder cancers were recruited via registries in 3 different regions in the United States. Patients were surveyed 3–5 years after diagnosis with the SF-12 and EPIC to evaluate general mental and physical health-related quality of life (HRQoL) and patient function and bother.ResultsWe analyzed responses from long-term prostate (n = 77) and bladder, colorectal, and lung cancer (n = 124) patients. In multivariate analysis, long-term local stage prostate cancer survivors had significantly higher SF-12 physical component scores but did not differ from long-term survivors of other cancers in terms of their SF-12 mental summary scores. Prostate survivors had similar mental, urinary, bowel, and sexual HRQoL compared to long-term survivors of other local stage cancers.ConclusionLong-term general and prostate-specific HRQoL was similar between local stage prostate and bladder, colorectal, and lung patients with cancer. Future research focusing on factors other than initial treatment and the cancer type per se may provide more meaningful information regarding factors that predict disparities on HRQoL among longer-term survivors of early stage male cancers. 相似文献