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目的 :将牙龈卟啉菌表面相关物质和外周血中淋巴细胞共同培养 ,观察淋巴细胞的凋亡情况。方法 :通过流式细胞仪对 2 0名健康人外周血中淋巴细胞培养组 (对照组 )与牙龈卟啉菌表面相关物质和淋巴细胞共同培养组 (实验组 )进行比较研究 ,观察淋巴细胞的凋亡情况。结果 :实验组与对照组相比 ,淋巴细胞凋亡数明显增多 (P <0 .0 5 )。结论 :牙龈卟啉菌表面相关物质诱导外周血中淋巴细胞凋亡 ,细菌诱导细胞凋亡在牙周可疑致病菌的致病机制中可能具有作用。 相似文献
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Effects of metronidazole on Porphyromonas gingivalis biofilms 总被引:2,自引:0,他引:2
T. L. Wright R. P. Ellen J.-M. Lacroix S. Sinnadurai M. W. Mittelman 《Journal of periodontal research》1997,32(5):473-477
Subgingival bacteria exist within a biofilm consisting of cells and extracellular matrix which may afford organisms protection from both antibiotics and components of the host immune system. MIC values for planktonic Porphyromonas gingivalis treated with metronidazole were compared with those obtained for the same strain in biofilms associated with hydroxyapatite (HA) surfaces. The treated biofilms were examined for growth and studied by scanning electron microscopy. A broth assay resulted in an MIC of 0.125 μg/ml for metronidazole against P. gingivalis . P. gingivalis biofilms exhibited growth after treatment with 20μg/ml metronidazole, which was 160 times the MIC for planktonic organisms. The results of this study indicate that biofilm-associated P. gingivalis may be resistant to metronidazole at concentrations which are usually attained by systemic administration. 相似文献
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人类免疫缺陷病毒-1(HIV-1)是以CD4+细胞缺损和功能障碍为主要表现的获得性免疫缺陷综合征(AIDS)的主要病原。AIDS与牙周病有密切的关系,HIV-1的感染被广泛认为是重型牙周病的危险因素,而近来研究发现牙周病可能是HIV-1感染宿主细胞、诱导潜伏的HIV-1复制的促进因素。牙龈卟啉单胞菌(Pg)是慢性牙周炎的主要致病菌之一,该文综述Pg对HIV-1致病性的影响。 相似文献
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BACKGROUND: Enamel matrix derivative (EMD) is used during periodontal surgery for the regeneration of periodontal tissue. It consists of the amelogenin fraction of porcine enamel matrix (AMEL) suspended in a vehicle of propylene glycol alginate (PGA). EMD-treated sites appear to heal with less inflammation. It has been suggested that antimicrobial properties of EMD might account for improved healing in vivo. The objectives of this study were: 1) to determine the antibacterial effects of EMD on the periodontal pathogen Porphyromonas gingivalis and 2) to establish the component(s) of EMD that are responsible for this effect. METHODS: The antimicrobial effects were determined in vitro using the broth dilution assay. P. gingivalis at a starting inoculum of 10(9) colony forming units/ml was treated with EMD, AMEL, and PGA in Hank's balanced salt solution (HBSS) for 1, 3, and 24 hours. The CFU/ml of P. gingivalis recovered on enriched Brucella blood agar was determined at 6 and 10 days. RESULTS: EMD (containing AMEL and PGA) or PGA alone eliminated recoverable CFUs of P. gingivalis. Interestingly, AMEL in HBSS increased recoverable CFUs from 8.62 log CFU/ml to 8.93 log CFU/ml. Further analysis of the dose response at concentrations of 0.3, 3, and 30 mg/ml of AMEL in HBSS revealed that only 30 mg/ml (clinical concentration) increased CFUs of P. gingivalis relative to baseline (from 8.8 log CFU/ml to 9.2 log CFU/ml in 3 hours). Additionally, AMEL was compared to a protein control bovine serum albumin (BSA) to determine whether this effect was unique to AMEL. A marked increase in recoverable CFUs occurred with the AMEL (increasing from 8.8 log CFU/ml to 9.47 log CFU/ml), but not with BSA. CONCLUSIONS: EMD possesses antimicrobial properties that can be attributed to the propylene glycol alginate vehicle. The amelogenin fraction of porcine enamel matrix in enamel matrix derivative (i.e., AMEL) is not antibacterial for P. gingivalis and was shown to increase recoverable CFUs. 相似文献
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Y. Asahi Y. Noiri J. Igarashi H. Asai H. Suga S. Ebisu 《Journal of periodontal research》2010,45(2):255-261
Asahi Y, Noiri Y, Igarashi J, Asai H, Suga H, Ebisu S. Effects of N‐acyl homoserine lactone analogues on Porphyromonas gingivalis biofilm formation. J Periodont Res 2009; doi: 10.1111/j.1600‐0765.2009.01228.x © 2009 John Wiley & Sons A/S Background and Objective: The gram‐negative anaerobic rod Porphyromonas gingivalis in oral biofilms is a primary etiological agent of periodontal disease. Biofilm formation of various gram‐negative bacteria is regulated by a quorum‐sensing circuit that relies on N‐acyl homoserine lactones (HSLs). Some synthetic N‐acyl HSL analogues act as quorum‐sensing inhibitors and suppress biofilm formation in Pseudomonas aeruginosa. Development of chemical control agents against oral biofilms is necessary, because until now, biofilms have been removed only by mechanical debridement. The present study investigated the effect of N‐acyl HSL analogues on P. gingivalis biofilm formation, with the aim of developing new drugs that inhibit oral biofilm formation. Material and Methods: A flow‐cell model was used for P. gingivalis biofilm formation. Seventeen synthetic N‐acyl HSL analogues were quantitatively assessed by spectrophotometry. The effects of three antagonistic compounds against P. gingivalis biofilm formation were further examined by confocal laser scanning microscopy, and investigated for primary attachment using spectrophotometry and phase contrast microscopy. Results: Ten out of 17 analogues affected P. gingivalis biofilm formation. Three out of 10 analogues significantly decreased biofilm‐forming cells (p < 0.05), and these biofilm structures were less well formed three‐dimensionally. There were no quantitative or qualitative differences in cell attachment between the control and the three analogue‐treated groups. Conclusion: Three synthetic N‐acyl HSL analogues inhibited biofilm formation in P. gingivalis. We suggest that these analogues influence the development stage of P. gingivalis biofilm formation. 相似文献
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目的 本实验研究右旋色氨酸、右旋亮氨酸、右旋酪氨酸和右旋蛋氨酸对牙龈卟啉单胞菌生物膜形成的影响。方法 用含有维生素K(1 μg/mL)和氯化血红素(5 μg/mL)的TSB培养基培养牙龈卟啉单胞菌W83,培养到对数生长期时1:100倍稀释后放入96孔板中,分别加入右旋色氨酸(5 mmol/L)、右旋亮氨酸(8.5 mmol/L)、右旋酪氨酸(3 μmol/L)和右旋蛋氨酸(2 mmol/L),厌氧培养7 d后结晶紫染色,双蒸水冲洗,干燥后乙醇脱色,紫外分光光度计检测。 结果 加入右旋色氨酸、右旋亮氨酸、右旋酪氨酸和右旋蛋氨酸及空白对照组的吸收值分别为3.70 ± 0.06, 2.74 ± 0.18, 0.71 ± 0.04,3.42 ± 0.02,和3.65 ± 0.01。与空白对照组相比较,加入右旋亮氨酸组吸收值小,并有统计学差异(P < 0.01)。 结论 右旋亮氨酸能够减少牙龈卟啉单胞菌生物膜形成。 相似文献
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Confocal scanning laser microscopy (CSLM) was used to visualize and quantify biofilm formation by the oral bacteria Streptococcus gordonii and Porphyromonas gingivalis , A saliva-coated glass coverslip under continuous bacterial challenge and conditions of low shear force was used to investigate attachment to the salivary pellicle and also the effect of cell-cell interactions on the extent of colonization and biofilm development. S. gordonii bound to the salivary pellicle and outcompeted P. gingivalis for attachment sites. Both P. gingivalis and S. gordonii failed to establish substantial biofilm formation independently. However, biofilm formation did occur subsequent to initial adherence of P. gingivalis to S. gordonii cells deposited on the salivary pellicle. The commensal species S. gordonii may. therefore, provide an attachment substrate for colonization and biofilm accretion by the potential pathogen, P. gingivalis. 相似文献
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Confocal scanning laser microscopy (CSLM) was used to visualize and quantify biofilm formation by the oral bacteria Streptococcus gordonii and Porphyromonas gingivalis. A saliva-coated glass coverslip under continuous bacterial challenge and conditions of low shear force was used to investigate attachment to the salivary pellicle and also the effect of cell-cell interactions on the extent of colonization and biofilm development. S. gordonii bound to the salivary pellicle and outcompeted P. gingivalis for attachment sites. Both P. gingivalis and S. gordonii failed to establish substantial biofilm formation independently. However, biofilm formation did occur subsequent to initial adherence of P. gingivalis to S. gordonii cells deposited on the salivary pellicle. The commensal species S. gordonii may, therefore, provide an attachment substrate for colonization and biofilm accretion by the potential pathogen, P. gingivalis. 相似文献
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Saito Y Fujii R Nakagawa KI Kuramitsu HK Okuda K Ishihara K 《Oral microbiology and immunology》2008,23(1):1-6
BACKGROUND/AIMS: Bacterial infection is a major cause of periapical periodontitis. Eradication of these microorganisms from apical lesions is essential to the success of endodontic treatment. The aim of this study was to clarify the molecular interaction between Fusobacterium nucleatum, Porphyromonas gingivalis and other microorganisms associated with periapical periodontitis. METHODS: Microorganisms isolated from periapical lesions were inoculated into type-I collagen-coated polystyrene microtiter plates and maintained at 37 degrees C under anaerobic conditions for 2 days, after which, the quantity of organized biofilm on the plates was evaluated by crystal violet staining. Growth enhancement via soluble factor was evaluated by separated coculture using a 0.4-mum membrane filter. RESULTS: F. nucleatum exhibited strong adherence to type-I collagen-coated polystyrene microplates. Biofilm formation by F. nucleatum was significantly enhanced by P. gingivalis. It was complemented by compartmentalized coculture with P. gingivalis. Enhancement of biofilm formation by P. gingivalis was only slightly reduced by inactivation of its autoinducer-2-producing gene luxS. CONCLUSION: The results suggest that P. gingivalis enhances biofilm formation by F. nucleatum by releasing diffusible signaling molecules other than autoinducer-2. 相似文献
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牙龈卟啉单胞菌研究进展 总被引:2,自引:0,他引:2
牙周炎是常见的口腔疾病之一,是中国成年人失牙的主要原因.牙龈卟啉单胞菌在引发牙周组织破坏过程中发挥着重要的作用,成为国内外学者研究的热点之一.本文就牙龈卟啉单胞菌与口腔疾病和其他全身性疾病的关系、牙龈卟啉单胞菌全基因组,牙龈卟啉单胞菌与牙龈上皮细胞的相互作用等研究作一综述,以拓宽牙龈卟啉单胞菌的研究思路,进血为牙周炎的... 相似文献
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The objective of this study was to investigate the ability of alendronate and taurine in inhibiting in vitro osteoclast differentiation induced by bacteria. Whole cell sonicates of Porphyromonas gingivalis were used as an osteoclast-stimulating factor in a mouse coculture system and differentiated osteoclasts were confirmed by tartrate-resistant acid phosphatase (TRAP) staining. Alendronate at the concentrations of 10(-7) M, 10(-6) M, and 10(-5) M and taurine at the concentrations of 4 mM, 8 mM, and 12 mM were used. The cytotoxic effects of alendronate and taurine were examined using methylthiazole-tetrazolium bromide (MTT) assay. The amounts of interleukin-6 (IL-6) in culture supernatants were also measured using ELISA. The sonicates of P. gingivalis at the concentration of 0.01-0.1 microg/ml significantly stimulated the formation of osteoclasts (p < 0.05). Alendronate (10(-5) M) and taurine (12 mM) significantly suppressed the sonicate-stimulated osteoclast formation. In MTT assay, no cytotoxic effects were evident in all concentrations of alendronate and taurine. Alendronate and taurine did not affect the amount of IL-6 induced by P. gingivalis sonicates. These data indicate that alendronate and taurine have inhibitory effects on bacteria-stimulated osteoclast formation in vitro and that this inhibitory mechanism is not related to the blocking of IL-6 production. 相似文献
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目的 研究群体感应抑制剂溴化呋喃酮对牙龈卟啉单胞菌生物膜形成的影响.方法 采用二倍稀释法,确定溴化呋喃酮对牙龈卟啉单胞菌的最低抑菌浓度(MIC).含无水乙醇的牙龈卟啉单胞菌菌悬液作为阴性对照组,牙龈卟啉单胞菌菌悬液为空白对照组,通过光密度值测定和扫描电镜观察,研究1/4M1C、1/2MIC、MIC、2MIC等不同浓度溴... 相似文献
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BACKGROUND: Dipeptide bestatin has been previously reported to selectively inhibit the growth of Porphyromonas gingivalis. The aims of this study were to investigate the mechanism of action of bestatin and to evaluate its effect on epithelial cells. METHODS: The inhibitory effect of bestatin on P. gingivalis was tested in vitro (culture medium) and in vivo (guinea pig model). Radiolabeled compounds were used to investigate the effect of bestatin on the uptake of amino acids and peptides. The cytotoxic effect of bestatin was evaluated using a keratinocyte cell line. RESULTS: The growth inhibition of P. gingivalis by bestatin was concentration-dependent. Even at high concentrations, compounds possessing a chemical structure or an aminopeptidase inhibitor activity related to bestatin had no effect on growth of P. gingivalis. When injected in the presence of P. gingivalis, bestatin was able to prevent the development of a necrotic abscess in a guinea pig model. Data were obtained suggesting that bestatin does not act on proteinases of P. gingivalis. Rather, bestatin was found to inhibit the intracellular uptake of radioactivity from 14C-labeled amino acids or heat-denatured type I collagen. This was not observed with a spontaneous mutant of P. gingivalis, whose growth was not affected by bestatin. In the second part of the study, bestatin was found to have no effect on epithelial cell viability in culture at concentrations effective on P. gingivalis. In addition, bestatin did not show effects on epithelial cell migration or production of gelatinases. CONCLUSIONS: This study suggests that bestatin selectively inhibits growth of P. gingivalis by affecting the intracellular uptake of amino acids and peptides, which serve as energy and nitrogen sources for this bacterial species. Bestatin has no cytotoxicity and may represent a therapeutic molecule for local treatment of P. gingivalis-associated periodontitis. 相似文献
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目的 了解牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)脂多糖(lipopolysaccharide,LPS)在巨噬细胞吞噬氧化低密度脂蛋白(oxidized low density lipopmtein,oxLDL)形成泡沫细胞过程中和形成后对凋亡基因的影响,以期了解Pg影响动脉粥样硬化的可能机制.方法 以oxLDL、oxLDL+Pg-LPS刺激人急性单核细胞白血病单核细胞株THP-1源性巨噬细胞,以及oxLDL诱导巨噬细胞形成的泡沫细胞.采用吖啶橙-溴化乙锭双染色观察细胞凋亡,聚合酶链反应(PCR)芯片检测11种动脉粥样硬化相关凋亡基因的变化,实时PCR检测p53、c-Myc和半胱氨酸天冬氨酸蛋白酶(caspase)-3基因的变化.结果 Pg-LPS提高了巨噬细胞吞噬oxLDL形成泡沫细胞过程中和形成后的细胞凋亡率,分别为(5.47±0.93)%、(7.50 4-0.54)%;PCR芯片检测显示泡沫细胞形成过程中Pg-LPS上调了B细胞淋巴瘤-白血病-2相关蛋白Al的转录(>2倍),泡沫细胞形成后上调了B细胞淋巴瘤-白血病-2和B细胞淋巴瘤-白血病-2相关蛋白A1的转录(>2倍);在泡沫细胞形成过程中提高了p53和caspase-3的转录水平[分别为(4.50×10-3±4.02×10-4)和(5.30×10-2±4.58×10-3)],抑制了c-Myc的转录水平(1.53×10-2±5.77×10-4);在泡沫细胞形成后降低了p53转录水平(4.23×10-3±5.85×10-4),促进了caspase-3的转录水平(6.00×10-2±6.08×10-3),P<0.05.结论 Pg-LPS影响了泡沫细胞形成过程中和形成后细胞中多种凋亡基因的转录,促进了凋亡的发生. 相似文献
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牙龈卟啉单胞菌与动脉粥样硬化 总被引:1,自引:0,他引:1
大量流行病学证据已经证实牙周病和动脉粥样硬化存在一定的相关性,目前的研究主要偏向于牙周病在动脉粥样硬化病因中的作用机制,牙周病的病原体如牙龈卟啉单胞菌是否为动脉粥样硬化的病原体尚不清楚.本文对牙龈卟啉单胞菌在动脉粥样硬化发生、发展过程中所起的作用作一综述. 相似文献
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Stress response of Porphyromonas gingivalis 总被引:2,自引:0,他引:2
The heat shock response of Porphyromonas gingivalis was examined by 1 - and 2-dimensional polyacrylamide gel electrophoresis after metabolic labeling with [14 C]-amino acids. When P. gingivalis cells were shifted from 37° C to 42° C, elevated synthesis of 4 proteins with the apparent molecular weight of 92, 80, 74, and 62 kDa was observed, and synthesis of a 50-kDa protein decreased during heat shock. The 74- and 62-kDa proteins were identified as homologs of Escherichia coli DnaK and GroEL respectively by Western immunoblotting. On 2-dimen-sional Western blots, 2 forms of DnaK and 4 forms of GroEL were identified due to their slightly different isolelectric points. dna K and gro EL gene homologs were identified in several P. gingivalis strains and some other black-pigmented oral species. DnaK and GroEL homolog proteins were induced when P. gingivalis cells were challenged by ethanol. These proteins were not induced by oxidative stress or change in pH. 相似文献
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Yoshimura F Murakami Y Nishikawa K Hasegawa Y Kawaminami S 《Journal of periodontal research》2009,44(1):1-12
Background and Objective: Research on Porphyromonas gingivalis , a periodontopathogen, has provided a tremendous amount of information over the last 20 years, which may exceed in part than that on other closely related members in terms of phylogenetic as well as proteomic criteria, including Bacteroides fragilis and B. thetaiotaomicron as major anaerobic, opportunistic pathogens in the medical field. In this minireview, we focused on recent research findings concerning surface components such as outer membrane proteins and fimbriae, of P. gingivalis .
Material and Methods: Elucidation of the surface components in P. gingivalis was especially difficult because outer membrane proteins are tightly bound to lipopolysaccharide and they are resistant to dissociation and separation from each other, even during sodium dodecyl sulfate–polyacrylamide gel electrophoresis, unless samples are appropriately heated. In addition, P. gingivalis is asaccharolytic and therefore a potent proteolytic bacterium, another factor causing difficulty in research. The study of the surface components was carefully carried out considering these unique features in P. gingivalis when compared with other gram-negative bacteria, including Escherichia coli and Pseudomonas aeruginosa .
Results: Separation of outer membrane proteins, and characterization of OmpA-like proteins and RagAB as major proteins, is described herein. Our recent findings on FimA and Mfa1 fimbriae, two unique appendages in this organism, and on their regulation of expression are also described briefly.
Conclusion: Surface components of P. gingivalis somehow have contact with host tissues and cells because of the outermost cell elements. Therefore, such bacterial components are potentially important in the occurrence of periodontal diseases. 相似文献
Material and Methods: Elucidation of the surface components in P. gingivalis was especially difficult because outer membrane proteins are tightly bound to lipopolysaccharide and they are resistant to dissociation and separation from each other, even during sodium dodecyl sulfate–polyacrylamide gel electrophoresis, unless samples are appropriately heated. In addition, P. gingivalis is asaccharolytic and therefore a potent proteolytic bacterium, another factor causing difficulty in research. The study of the surface components was carefully carried out considering these unique features in P. gingivalis when compared with other gram-negative bacteria, including Escherichia coli and Pseudomonas aeruginosa .
Results: Separation of outer membrane proteins, and characterization of OmpA-like proteins and RagAB as major proteins, is described herein. Our recent findings on FimA and Mfa1 fimbriae, two unique appendages in this organism, and on their regulation of expression are also described briefly.
Conclusion: Surface components of P. gingivalis somehow have contact with host tissues and cells because of the outermost cell elements. Therefore, such bacterial components are potentially important in the occurrence of periodontal diseases. 相似文献