一种西红花掺伪品鉴别

目的研究一种新出现的西红花伪品的鉴别方法。方法通过观察药材性状、显微特征和薄层色谱,对真伪品进行比较鉴别。结果真品与伪品在药材性状、显微特征和薄层色谱3个方面均有着明显差异。结论 3种方法相互结合,能有效地鉴别西红花真假。 更多还原     

五味子中掺伪品南五味子的鉴别

目的:报道一种五味子掺伪现象及对掺伪品南五味子的鉴别,为有效区分真伪品提供实验依据.方法:采用薄层色谱、紫外光谱和高效液相色谱等方法鉴别.结果:二者有明显的鉴别特征.结论:本文提供的鉴别方法能够准确地鉴别五味子中的掺假伪品南五味子.     

一种蒲黄伪品的鉴别

目的研究一种新出现的蒲黄伪品的鉴别方法。方法通过观察药材性状、显微特征和薄层色谱,对蒲黄真伪品进行比较鉴别。结果真品与伪品在药材性状、显微特征、理化现象和薄层色谱四方面均有显著差异。结论4种方法相互结合,能有效地鉴别蒲黄真伪。     

青黛与其一种伪品的鉴别

目的：鉴别临床应用的一种可疑“青黛”的真伪。方法：采用显微,薄层层析,吸收光谱及理化鉴别法对其性状,显微及理化特性进行鉴别并与真品比较,结果“青黛”从外形,颜色及一般化学性质上与真品不同;薄层层析按药典规定方法“青黛”无斑点吸收光谱鉴别真品在６０８ｎｍ有一最大吸收峰,伪品在４００￣８００ｍｍ之间无吸收峰,进一步鉴别伪品中主要含有Ｆｅ２Ｓ３,ＦｅＳ等对人体有害的掺假成分。结论：“青黛”为一种伪品,本     

地奥心血康胶囊鉴别方法探讨

目的探讨地奥心血康胶囊的专属鉴别方法。方法以黄山药皂苷提取物作对照品,甲醇作溶剂,采用薄层色谱法、吸收光谱法进行鉴别试验,并对正、伪品的性状特征和显微鉴别进行对比。结果正品地奥心血康胶囊在薄层色谱图中主斑点的颜色、位置,以及在220~350nm波长范围内的吸收光谱图与对照品相同,而伪品与对照品不同;伪品与正品的性状特征及显微鉴别有明显区别。结论显微鉴别、薄层色谱及吸收光谱法操作简便、快速、专属、准确,是控制地奥心血康胶囊内在质量的有效方法。     

牛黄及其伪品的鉴别

因天然牛黄紧缺,价格昂贵,常有掺假牛黄。本文采用性状鉴别,显微鉴别,理化鉴别方法。鉴别牛黄的真品及常见伪品。方法简便易行,有助于保证牛黄临床应用的安全。     

某伪制羚羊角片的定性鉴别与研究

目的对某种伪制羚羊角纵镑片进行定性鉴别,并描述其外观特征。方法①通过真伪品的紫外光谱及薄层色谱的比对实验,为伪品确认提供依据。②描述伪品的外观性状及与真品差异。结果①紫外光谱：在210～230nm之间,真品有吸收肩峰,而伪品没有肩峰;薄层色谱：在Rf值约0．2处,伪品比真品多出一个斑点。②两者的形态、环脊轮廓、内外表面色泽、触摸手感等外观特征均有差异。结论本文介绍的外观特征能有效识别该伪制羚羊角纵镑片。     

西红花及其伪品的生药鉴别

目的 鉴别西红花真伪,保证临床用药质量.方法 根据西红花正品及不同种类伪品的外观性状特征,鉴别西红花的真伪.结果 目前市场上西红花药材中常出现混伪品,如红花、莲须、玉蜀黍、染色伪制品等.结论正品西红花与混伪品性状差异明显,为确保西红花质量和临床疗效,建议购买西红花药材时应前往正规、大型、可信度高的老字号企业进行购买.     

柴胡与其混淆品小叶黑柴胡及锥叶柴胡的鉴别

目的：寻找柴胡与其混淆品小叶黑柴胡及锥叶柴胡的有效坚别方法方法：采取观察药材性状、根的横切面显微特征和薄层色谱的不同，对真品与混淆品加以坚别。结果：真品与混淆品在上述三个方面均有着明显差别结论：将药材性状的经验坚别与显微或色谱方法相互结合，能有效地鉴别柴胡真伪。     

市售蒲黄商品的品质考察与鉴别

通过对市售蒲黄商品的取样研究发现,目前蒲黄纯品较少,掺假品和伪品多见。本文对市售蒲黄商品进行了比较鉴别,确立蒲黄正伪品的鉴别特征,为蒲黄的品质评价提供相关依据。     

回归正交设计优化西红花苷的提取工艺

目的研究从西红花中提取西红花苷的最佳工艺条件。方法以西红花苷的提取率为指标,在使用单因素试验方法考察乙醇浓度、提取时间、提取温度对西红花中西红花苷提取率的基础上,应用回归正交方法建立3元2次方程优化西红花苷的提取工艺。结果最佳的提取工艺是：乙醇浓度52％,提取时间30min,提取温度30℃。此条件下,提取率为0．6435mg·g-1。结论在优化的提取工艺下,西红花中西红花苷提取率较高。     

番红花不同药用部位的镇痛和抗炎活性比较研究

目的:扩大名贵药用植物番红花的药用资源,寻找可以替代传统药用部位(柱头)的新药用部位.方法:分别采用醋酸致小鼠扭体和二甲苯致小鼠耳肿胀药理模型,分别以吲哚美辛和地塞米松为镇痛和抗炎药效学实验的阳性对照药,观察番红花球茎、顶芽、侧芽和柱头的乙醇和水提取物的镇痛和抗炎活性.结果:在镇痛实验中,与空白对照组相比,顶芽醇提取物和水提取物、柱头的醇提物和水提物以及侧芽的醇提物在高剂量时(相当于2.0 g生N/kg)都具有显著的镇痛活性(P＜0.05或P＜0.01);球茎的醇提物和水提物、侧芽醇提取物的低剂量和中剂量以及水提物均未见有镇痛活性;在抗炎试验中,与空白对照组相比,番红花柱头醇提物和侧芽醇提物均显示出显著的抗炎活性(P＜0.05或P＜0.01).项芽醇提物在低剂量(相当于0.75 g生药/kg)时也具有显著的抗炎活性(P＜0.05).球茎醇提取物和水提物及顶芽、侧芽和柱头的水提物对二甲苯致小鼠耳肿胀没有显著性影响.结论:以番红花的顶芽、侧芽替代传统药用部位柱头,分别用于镇痛和抗炎作用有潜在的应用价值,值得深入研究.     

西红花酸药理作用的研究进展

西红花的化学成分包括类胡萝卜素类及其糖苷类、黄酮苷类、氨基酸等.西红花酸是主要有效成分之一,本文对西红花酸的药理作用进行了较全面的综述,为合理开发利用西红花提供参考.     

注射用西红花总苷粉针剂的研制

目的 :探讨注射用西红花总苷的处方组成和制备工艺。方法 :以甘露醇、氯化钠、山梨醇为骨架材料 ,制备注射用西红花总苷粉针剂 ;采用HPLC测定西红花总苷粉针剂的含量。结果 :最佳处方为 :甘露醇 5 g ,西红花 5 g(1∶1) ,5 0mg西红花总苷粉针剂能在 2mL注射用水中迅速溶解。 3批样品含量为 99.8% ,10 0 .1% ,98.83% ,符合新药申报要求。结论 :本研究方法制备工艺合理 ,所得制剂质量可控 ,性质稳定 ,可满足临床用药需求     

高效液相色谱梯度洗脱法测定不同中国产西红花药材中西红花苷Ⅰ、Ⅱ的含量

目的建立高效液相色谱梯度洗脱法测定国产西红花中西红花苷Ⅰ和西红花苷Ⅱ的含量。方法采用反相高效液相色谱法,以Agilent ZORBAX StableBond SB-Aq（4.6 mm×250 mm,5μm）柱为色谱柱;流动相为0.01%甲酸（A）-乙腈（B）,进行梯度洗脱,0～8 min（75%～70%A）,8～10 min（70%～60%A）,10～25 min（60%～30%A）;流速：1.0 mL.min-1;进样量：5μL;测定波长：440 nm;柱温：30℃。结果西红花苷Ⅰ和西红花苷Ⅱ的保留时间大约分别为5.71 min和8.83 min。以峰面积对进样浓度线性回归,西红花苷Ⅰ回归方程：Y=24.93X-0.254 3,r=1.000,线性范围3.125～200μg.mL-1,西红花苷Ⅱ回归方程：Y=36.11X＋8.370,r=1.000,线性范围3.125～200μg.mL-1。西红花苷Ⅰ和西红花苷Ⅱ的回收率分别为101.1%和100.2%,RSD分别为1.7%和1.4%。结论本方法可用于测定不同国产西红花药材中西红花苷Ⅰ、Ⅱ的含量,本方法操作简便,结果准确。     

Authentic identification of stigma Croci (stigma of Crocus sativus) from its adulterants by molecular genetic analysis

Stigma Croci, stigma of Crocus sativus L., is a precious traditional Chinese medicine, which is commonly used to activate blood circulation and to dissipate blood stasis. Three plant species, Carthamus tinctorius L., Hemerocallis fulva (L.) L. and Hemerocallis citrina Baroni, could carry the name Stigma Croci in the commercial markets of South East Asia. However, C. sativus is the only one that has proven its effectiveness, while the others could act as adulterants. The authentic identification of C. sativus on the market is difficult. By using molecular genetic method, the spacer domains of 5S-rRNA were cloned from the genomic DNAs that were isolated from C. sativus, C. tinctorius, H. fulva and H. citrina. The cDNAs encoding the spacer domains, about 300 to 500 bp, were sequenced. The nucleotide sequences of these four species showed great diversity, which could serve as markers for authentic identification of Stigma Croci to distinguish from its substitution and counterfeit.     

LC-DAD-MS (ESI+) analysis and antioxidant capacity of crocus sativus petal extracts

In this study, various fractions isolated from the petals of Crocus sativus were assessed at first for their phenolic content both qualitatively and quantitatively and secondly for their antioxidant activity. The phytochemical analysis was carried out by LC-DAD-MS (ESI (+)) whereas the antioxidant potential was evaluated by applying two methodologies, the DPPH. radical scavenging activity test and the Co(II)-induced luminol chemiluminescence procedure. According to data obtained from these antioxidant tests, the diethyl ether, ethyl acetate and aqueous fractions demonstrated the strongest antioxidant capacity. Interestingly, the major constituents identified in these fractions correspond to kaempferol, quercetin, naringenin and some flavanone and flavanol derivatives glycosylated and esterified with phenylpropanoic acids. In addition, the presence of some nitrogen-containing substances, as well as other phenolics and phenylpropanoic derivatives was also traced. The identification and structural elucidation of all substances isolated in this study was achieved by both comparing available literature data and by proposed fragmentation mechanisms based on evaluating the LC-DAD-MS (ESI (+)) experimental data. The quantitative analysis data obtained thus far have shown that Crocus sativus petals are a rich source of flavonoids. Such a fact suggests that the good antioxidant capacity detected in the various fractions of Crocus sativus petals could be attributed to the presence of flavonoids, since it is already known that these molecules exert antioxidant capability. The latter, along with the use of Crocus sativus in food and pharmaceutical industry is discussed.     

藏红花药理作用研究进展

藏红花是我国一种应用广泛的名贵中药与民族药,具有很好的活血化瘀、散郁开结作用。藏红花中主要含有苦藏花素、藏红花素等成分,藏红花及其提取物主要用于防治心血管疾病以及与衰老相关的疾病,并且有多种保健品和中成药在国内市场上销售。主要综述近年来有关藏红花及其有效成分防治动脉粥样硬化、保肝护胆、干预肿瘤、调节免疫功能、保护肾脏、防治骨质疏松等方面药理作用的研究进展,为更好地利用该药用植物资源、开发民族药提供一定的帮助。     

新通滞苏润江胶囊质量标准研究

目的:建立新通滞苏润江胶囊的质量标准。方法:采用薄层色谱(TLC)法对方中番泻叶、西红花、秋水仙进行定性鉴别;采用高效液相色谱法测定该制剂中没食子酸含量。结果:TLC中斑点清晰、分离度好。没食子酸的进样量在0.1557～1.4013μg范围内与峰面积积分值呈良好线性关系(r=0.9998);平均回收率为101%,RSD=1.64%(n=9)。结论:所建标准可用于新通滞苏润江胶囊的质量控制。     

Effects of saffron and its constituents, crocin-1, crocin-2, and crocetin on α-synuclein fibrils

Journal of Natural Medicines - Saffron, the stigma of Crocus sativus Linné (Iridaceae family), has been known to inhibit aggregation of β-amyloid, a nerve tissue protein. α-Synuclein...