视神经管及眶上裂区巨微解剖学研究

视神经管及眶上裂为颅眶沟通的２个重要骨性孔隙。视神经及眼动脉经视神经孔出入眶，而所有通过海绵窦的脑神经及眼静脉均经眶上裂出入眶。虽然关于海绵窦及眼眶的显微解剖学研究已报道许多［１～８］，但作为两者交通的视神经管及眶上裂区的系统显微解剖学研究却很少［９...     

眼静脉与海绵窦前间隙的显微外科解剖学

目的：为临床治疗眶上裂区域的肿瘤和颈内动脉海绵窦瘘提供显微外科解剖学依据。方法：应用成人头颅湿标本在16倍显微镜下，观测海绵窦前间隙、眶内引流静脉及相互之间的关系，和胎儿眶上裂区血管铸型标本。结果：眼上静脉在眶内分为3段，各段直径平均为(2．13～2．57)mm，眼上、下静脉在眶内汇合形成长约(4．35～4．97)mm的眼静脉总干，眼静脉总干从海绵窦前间隙前方或前下方与海绵窦前间隙相连，眼静脉总干呈纺锤型。结论：本文结果为临床选择该区域适当的手术入路打下基础，经眼上静脉治疗颈内动脉海绵窦瘘是可行的。     

岩静脉的解剖学特征及临床意义

目的：为临床桥脑小脑角区的手术提供岩静脉的应用解剖学资料。方法：经甲醛固定的成人头部标本２６例,手术显微镜下解剖观察岩静脉形态、行径及其与三叉神经的毗邻关系。结果：①岩静脉有１～３支,长度２．９±２．０ｍｍ,外径２．３±１．０ｍｍ;②岩静脉位于三叉神经根背外方者占９２．５％;岩静脉与神经根的最短距离５．０±３．８ｍｍ,最长距离７．１±４．１ｍｍ;静脉对神经根有压迫的占１５．４％;③岩静脉距横窦与乙状窦交角处的距离为３１．１±５．２ｍｍ。结论：岩静脉与三叉神经根关系密切,可压迫三叉神经根引起三叉神经痛。     

活体肾上腺血管的解剖观测及临床意义

目的：研究活体肾上腺血管的应用解剖。方法：对１８例２４侧肾上腺手术时分离出血管，测量血管外径及长度，对６个完整肾上腺灌注染色。结果：右膈下动脉从下腔静脉右缘至末支肾上腺上动脉长８．５（７．５～１１．０）ｃｍ，外径２．５～３．０ｍｍ；左膈下动脉从腹主动脉左缘至末支肾上腺上动脉长８．０（７．０～９．０）ｃｍ，外径２．５～３．１ｍｍ；肾上腺上动脉１～６支，均源于膈下动脉。肾上腺中动脉右侧长１．７～２．２ｃｍ，外径１．０～１．５ｍｍ；左侧长１．５～２．０ｃｍ，外径１．０～１．５ｍｍ。肾上腺下动脉两侧基本相同，长１．５～２．０ｃｍ，外径１．０～２．０ｍｍ。肾上腺中心静脉右侧长０．４～０．８ｃｍ，左侧长３．０～４．０ｃｍ，外径３．０～４．０ｍｍ。结论：肾上腺血管加部分膈下血管的长度可供带血管蒂肾上腺转位治疗柯兴氏病；活体切取供移植的肾上腺；选择性肾上腺血管造影等。     

海绵窦的断层解剖及其临床意义

目的：为海绵窦的影像学诊断和外科学治疗提供解剖学依据。方法：利用头颅连续断层标本,观察海绵窦在各断面上的位置、大小、形态、内部结构和毗邻。结果：海绵窦平均长２１．１±３．２ｍｍ,宽８．７±０．７ｍｍ,外侧壁高１４．７±３．３ｍｍ,内侧壁高１６．４±１．３ｍｍ。颈内动脉和展神经位于海绵窦壁内,脑神经Ⅲ～Ⅴ位于海绵窦外侧壁内,这些神经与颈内动脉之间有恒定的位置关系。在颈内动脉的内侧、下方及外下方可出现静脉间隙,出现率分别为６９％、４３％和１００％。海绵窦外侧壁硬脑膜可分为浅、深两层;浅层为形成颅中窝内侧壁的硬脑膜,深层构成脑神经Ⅲ、Ⅳ、Ⅴ１和Ⅶ２的鞘及鞘之间的网状膜。结论：海绵窦静脉间隙和外侧壁脑膜结构的了解有助于影像学和显微外科学对海绵窦病变的评价和手术切除     

腓肠内外侧血管解剖特点及其在小腿创伤修复中的意义

目的：为小腿创伤皮瓣移植修复与腓肠内、外侧血管吻合提供解剖学基础。方法：在７０侧成人下肢标本上解剖观测了腓肠内、外侧血管的长度、外径、血管神经的毗邻关系及其形态特点。结果：①腓肠内、外侧动脉起于动脉，伴行静脉注入静脉；②动脉起点至入肌段的长度内、外侧分别为４．１±０．７（１．８～６．４）ｃｍ、３．７±０．５（２．０～６．５）ｃｍ；③肌门处内、外侧动脉的外径分别为２．２±０．１（１．１～３．４）ｍｍ、２．０±０．１（０．９～３．１）ｍｍ；④肌门处伴行静脉有１～２条，内、外侧静脉外径为２．５±０．３（１．８～４．５）ｍｍ，２．３±０．３（１．５～４．１）ｍｍ。结论：腓肠内、外侧血管恒定、径粗、较长，是小腿创伤皮瓣移植修复受区可供应用的吻合血管     

以第1或第2穿动脉为蒂骨瓣转位重建股骨距的应用解剖

目的：为带血管蒂骨瓣转位重建股骨距提供解剖学依据。方法：３０侧经动脉内灌注红色乳胶的成人下肢标本，对第１、２穿动脉起始、走行、分支分布和骨膜支进行详细的解剖学观察。结果：股深动脉在小转子尖下４．５±１．３ｃｍ、９．３±２．７ｃｍ处分别发出第１、２穿动脉，外径分别为２．８±０．７ｍｍ、２．４±０．６ｍｍ。穿动脉发出１～３支外径在１．０ｍｍ以上的肌骨膜支，分布于股骨后部中上段骨膜。结论：可以第１或第２穿动脉为蒂设计股骨瓣，可转位重建股骨距或修复股骨颈。     

股骨内侧髁骨皮瓣移植重建下颌的应用解剖

在４０侧成人下肢标本上，解剖观测了膝降动脉及其伴行静脉。膝降动脉起点距内上髁平均９．１ｃｍ，外径１．８ｍｍ，可游离长度７．７ｃｍ。该动脉以其３支合干型为多见（６５．０％）。其伴行静脉压扁宽度平均２．６ｍｍ。关节支外径为１．４ｍｍ，可游离长度达６．７ｃｍ，分布于内侧髁及膝内侧皮肢，隐支外径平均１．２ｍｍ，可游离长度达９．６ｃｍ，分布于小腿内上１／３￣１／２皮肤。以膝降血管为蒂设计股骨内侧髁骨皮瓣对修     

旋股外侧血管横支大转子骨瓣修复股骨中上段的解剖及应用

目的：为股骨干骨缺损、骨折骨不连修复提供新的手术方法，方法：在４０侧标本上对旋股外侧动脉横支进行解剖学观察，设计了以该血管为蒂大转子骨瓣转位修复股骨干中、上段及股骨头颈部骨折、骨缺损。结果：该动脉外径２．５ｍｍ，长度５．１ｃｍ，在股外侧肌深面和外缘发出２～４支外径在０．４～１．１ｍｍ的骨膜支到大转子前外侧，供应范围３．５ｃｍ×２．０ｃｍ×３．５ｃｍ。结论：以旋股外侧动脉横支为蒂大转子骨瓣移位修复股骨中段或上段骨缺损具有可行性，临床应用１５例，疗效满意。     

股部皮神经营养血管皮瓣的应用解剖

目的：为股部皮神经及其营养血管为蒂的岛状皮瓣提供解剖学基础。方法：８侧经动脉灌注红色乳胶成人新鲜下肢标本，解剖观测股部主要皮神经血供及其筋膜皮支的分布范围；２侧成人新鲜下肢标本经墨汁灌注，皮肤脱水透明后观测各皮神经营养血管的分支分布及吻合。结果：①股外侧皮神经有营养动脉２～６支，超始外径１．０±０．４ｍｍ，其筋膜皮支分布达神经内侧４．１ｃｍ，外侧５．９ｃｍ；②股前皮神经有营养动脉１～５支，外径１．０±０．３ｍｍ，其筋膜皮支分布达神经内侧２．４ｃｍ，外侧３．４ｃｍ；③股后皮神经有营养动脉１～３支，外径０．９±０．３ｍｍ，其筋膜皮支分布达神经内侧３．９ｃｍ，外侧３．２ｃｍ。结论：以股部各主要皮神经及其营养血管为蒂可沿其皮神经走行设计切取顺行或逆行岛状皮瓣     

Schizophrenia in a North Swedish geographical isolate, 1900–1977. Epidemiology, genetics and biochemistry

Over 200 schizophrenic patients belonging to three major and interrelated pedigree complexes have been investigated over the past 30 years in a North Swedish geographically isolated population, presently numbering about 6,000. An intensive investigation of a number of biochemical correlates and genetic markers in a few selected families belonging to one of the major pedigrees has indicated new strategies for the current research program.
Schizophrenia, as defined operationally, is significantly associated with decreased activities of two enzymes (1) blood platelet monoamine oxidase, (2) plasma dopamine-β-hydroxylase, and (3) with the genetic marker Gc² (group specific antigen). Both enzymes are subject to genetic variation. A positive score for linkage between schizophrenia and low plasma DBH activity has been calculated, but, so far, available data are insufficient for discrimination between linkage and partial contribution of genetically controlled low plasma DBH to the pathogenesis of the disease. Alternatively, both mechanisms could be involved.
As a model for continued research, schizophrenia is explained as based on a double dominant-recessive genotype (Aabb), representing a vulnerability which in about 50 % of cases develops into clinical schizophrenia. It is suggested that the dominant mutation (A) operates on or affects MAO activity, and that the recessive genotype (bb) is instrumental in low variates of DBH activity and very likely such variates within the normal range of physiological variation. Moreover, it is suggested that the combined effects of MAO- and DBH-reduced efficiency on the metabolism of e.g. dopamine could be an essential pathogenic mechanism for the schizophrenic illness which is segregating in this population.     

Renal dysplasia and asplenia in two sibs

A family is reported in which two sibs, one male and the other female, both died within 24 hours of birth with enlarged polycystic kidneys. Postmortem histology in the second child showed gross renal dysplasia. In both children the pancreas was enlarged, nodular and cystic but the liver appeared macroscopically normal. In the second child, histological examination confirmed pancreatic fibrosis with cystic dilation of ducts, but showed portal fibrosis with bile duct proliferation in the liver.
This combination of findings is very reminiscent of those in a girl and her brother reported by Ivemark et al. (1959). The children reported here also showed absence or hypoplasia of the spleen, cardiac anomalies and other features of the Ivemark syndrome (Ivemark 1955), a quite different, usually sporadic, congenital disorder. It is suggested that the children described here have a distinct lethal congenital disorder, probably inherited in an autosomal recessive manner.     

The dominant diseases of modernized societies as omega-3 essential fatty acid deficiency syndrome: Substrate beriberi

About 1900, modern food selection and processing caused widespread $\text{epidemics}$ of the B vitamin deficiency diseases of beriberi and pellagra which, for genetic reasons, often expressed as different diseases ranging from bowel and heart disease to dermatoses and psychoses. But the B vitamins merely help convert essential fatty acids (EFA) into the prostaglandin (PG) tissue regulators and it now turns out that, through hydrogenation, milling and selection of w3-poor southern foods, we have also been systematically depleting, by as much as 90%, a newly discovered trace Nordic EFA (w3) of special importance to primates and sole precursor of the PG3(4) series, even as a concurrent fiber deficiency increases body demand for EFA. Since substrate EFA is processed by many B vitamin catalysts, an EFA deficiency will mimic a $\text{panh}$ypovitaminosis B, i.e., a mixture of $\text{substrate}$ beriberi and $\text{substrate}$ pellagra resembling vitamin beriberi and pellagra but exhibiting as even more diverse $\text{endemic}$ disease. This would consitute a second stage of the Modern Malnutrition and explain why some workers now hold the dominant diseases of modermized societies to be new, nutritionally based, pellagraform yet lipid-related and to range, once again, from heart disease to psychosis. It is an assumption that our dominant diseases are unrelated to each other or are merely revealed by our diagnostic acumen and therapeutic success; and that hydrogenating millions of tons of food oils annually, to destroy the rancidity producing w3-EFA, is safe for $\text{primates}$. Extensive beriberiform disease is reported here in 32 typical cases taken from medical practice which responds strikingly to linseed oil supplements (60% w3-EFA) in confirmation of identical results in Capuchins.     

'Very sore nights and days': the child's experience of illness in early modern England, c.1580-1720

Sick children were ubiquitous in early modern England, and yet they have received very little attention from historians. Taking the elusive perspective of the child, this article explores the physical, emotional, and spiritual experience of illness in England between approximately 1580 and 1720. What was it like being ill and suffering pain? How did the young respond emotionally to the anticipation of death? It is argued that children’s experiences were characterised by profound ambivalence: illness could be terrifying and distressing, but also a source of emotional and spiritual fulfilment and joy. This interpretation challenges the common assumption amongst medical historians that the experiences of early modern patients were utterly miserable. It also sheds light on children’s emotional feelings for their parents, a subject often overlooked in the historiography of childhood. The primary sources used in this article include diaries, autobiographies, letters, the biographies of pious children, printed possession cases, doctors’ casebooks, and theological treatises concerning the afterlife.     

Guidelines for the Validation and Use of Immunoassays for Determination of Introduced Proteins in Biotechnology Enhanced Crops and Derived Food Ingredients

Recent advancements in agricultural biotechnology have created a need for analytical techniques to determine introduced proteins in crops enhanced through modern biotechnology techniques. These proteins are expressed in plant tissues and may be present in food ingredients. Immunoassays are ideally suited for protein detection and may be used as both quantitative and threshold methods. Microplate ELISA and lateral flow devices are two of the most commonly used immunoassay formats for agricultural biotechnology applications. This paper provides general background information and a discussion of criteria for the validation and application of immunochemical methods to the analysis of proteins introduced into plants and food ingredients using biotechnology methods. It is the result of a collaborative effort of members of the Analytical Environmental Immunochemical Consortium. This collaborative effort represents the combined expertise of several organizations to reach consensus on establishing guidelines for the validation and use of immunoassays. Further, the paper offers developers and users a consistent approach to adopting the technology as well as aid in producing accurate and meaningful results.     

Ultracryotomy: development and application (with examples from the yeast cytology) (author's transl)

The preparation steps usually necessary for obtaining ultrathin frozen sections of biological material (chemical prefixation, enclosing, cryoprotective treatment, freezing, sectioning, and post-staining the sections for transmission electron microscopy) are submitted to a critical analysis. The application of cryo-ultramicrotomy, in particularly for cytochemical purposes, is reviewed. Fundamental considerations of chemical prefixation and poststaining are supported by examples from yeast cytology. Furthermore, the efficiency of the cryo-ultramicrotomy (electron optical resolution of ultrastructural details) is demonstrated on yeast cells and protoplasts.     

HLA in North Colombia Chimila Amerindians

HLA-A,-B,-C,-DRB1 and -DQB1 alleles have been studied in Chimila Amerindians from Sabana de San Angel (North Colombian Coast) by using high resolution molecular typing. A frequent extended haplotype was found:HLA-A*24:02-B*51:10-C*15:02-BRB1*04:07-DQB1*03:02 (28.7%) which has also been described in Amerinndian Mayos Mexican population (Mexico, California Gulf, Pacific Ocean). Other haplotypes had already been found in Amerindians from Mexico (Pacific and Atlantic Coast), Peru (highlands and Amazon Basin), Bolivia and North USA. A geographic pattern according to HLA allele or haplotype frequencies is lacking in Amerindians, as already known. Also, five new extended haplotypes were found in Chimila Amerindians. Their HLA-A*24:02 high frequencies characteristic is shared with aboriginal populations of Taiwan; also, HLA-C*01:02 high frequencies are found in New Zealand Maoris, New Caledonians and Kimberly Aborigines from Australia. Finally, this study may show a model of evolutionary factors acting and rising one HLA allele frequency (-A*24:02), but not in others that belong to the same or different HLA loci.     

The case for allele‐specific recognition by the TCR

There is a sharp difference in how one views TCR structure–function–behaviour dependent on whether its recognition of major histocompatibility complex‐encoded restriction elements (R) is germline selected or somatically generated. The generally accepted or Standard model is built on the assumption that recognition of R is by the V regions of the αβ TCR, which is not driven by allele specificity, whereas the competing model posits that recognition of R is allele‐specific. The establishing of allele‐specific recognition of R by the TCR would rule out the Standard model and clear the road to a consideration of a competing construct, the Tritope model. Here, the case for allele‐specific recognition (germline selected) is detailed making it obvious that the Standard model is untenable.     

The universal muscular chamber. A basic unit of the genitourinary, cardiovascular, gastro-intestinal, skeletal, cutaneous, respiratory and other systems, endocameral hypertension; and spasm, the key to their idiopathic diseases and arthropathies

Starting with the integument, we see many organs are contractile sacs or multiples thereof, which tubes or bags constitute the major part of the entire body. Recognition of this basic unit and its characteristics sheds new light, individually and collectively, on many disorders previously considered unrelated. Muscular tears and perforations develop in the walls of these chambers, being no way peculiar to those organs, wherein, hydrochloric acid occurs. So, it is not necessary to explain the absence of excessive acid from patients who exhibit holes in the gastric, uterine, aortic, duodenal, rectal, pulmonary, retina, and other walls. Muscle, not acid is the great common factor relating idiopathic disorders in the gastrointestinal tract to each other and to similar diseases in other systems. When the units are linked together, the lesions tend to appear as arthropathies, i.e. at the joints. Rephrasing common-place observations, frees us from conventional, conceptual cul-de-sacs. An observation is only as good as its interpretation, so all possibilities must be considered, otherwise, we will remain blinded by our misconceptions.