miR-124通过靶向调控DNMT3B抑制胃癌细胞增殖

目的明确miR-124是否通过靶向调控DNA甲基化转移酶3B(DNA methyltransferase 3B,DNMT3B)表达而抑制胃癌细胞增殖能力,从而揭示miR-124的抑瘤分子机制。方法采用MTT检测miR-124对人胃癌MKN-45细胞的增殖能力;构建DNMT3B 3’UTR-荧光素酶报告载体,通过荧光素酶报告检测观察miR-124对DNMT3B3’UTR-荧光素酶活性的影响;将miR-124 mimics转染胃癌细胞MKN-45,采用Western blot检测DNMT3B表达水平。结果 MTT结果显示,在转染miR-124 mimics 24、48和72 h后OD值(0.264±0.023、0.377±0.041、0.524±0.029)分别与对照组(0.414±0.051、0.619±0.065、0.898±0.072)比较,差异均有显著性(均P<0.05),且具有时间依赖性;荧光素报告载体系统证实DNMT3B是miR-124直接调控的靶基因。Western blot结果显示,miR-124可抑制DNMT3B蛋白的表达。结论 miR-124通过靶向调控DNMT3B的表达而抑制胃癌细胞增殖能力。     

同型半胱氨酸致泡沫细胞形成过程中miR-148a与DNMT1相互作用的研究

【目的】 探讨DNMT1和其特异性miRNA二者在Hcy致泡沫细胞形成过程中的作用,阐明特异性miRNA与DNMT1相互调控的分子机制。 【方法】 培养THP-1单核源性泡沫细胞,油红O染色鉴定;生物信息学预测调控DNMT1的特异性miRNA;运用qRT-PCR和Western Blot检测不同浓度Hcy(0、50、100、200、500 μmol/L)及100 mol/L Hcy+叶酸+维生素B₁₂(H+F+V)干预泡沫细胞后,miR-148a和DNMT1的表达。使用慢病毒miR-148a mimic和inhibitor分别过表达和抑制miR-148a,检测其对DNMT1表达的影响;针对miR-148a与DNMT1特异性结合的碱基位点,构建野生型(Wt)和突变型(Mut)的3'UTR荧光素酶报告质粒,与miR-148a mimic共转染,运用双荧光素酶报告基因检测系统检测DNMT1荧光素酶活性。采用胆固醇检测试剂盒分析miR-148a过表达或抑制后对细胞内胆固醇含量的影响。用100 mol/LHcy干预泡沫细胞,通过甲基化特异性PCR(nMS-PCR)检测miR-148a启动子区甲基化改变,同时构建DNMT1重组质粒并感染泡沫细胞,检测DNMT1过表达后miR-148a启动子区DNA甲基化变化。 【结果】 分析提示调控DNMT1的特异性miRNA为miR-148a;不同浓度Hcy干预泡沫细胞后,DNMT1表达降低,miR-148a表达升高,以100 μmol/L Hcy组改变最为明显,差异均有显著性(P<0.05);给予100 μmol/L Hcy+叶酸+维生素B12后上述情况改善(P<0.05)。过表达miR-148a后DNMT1 mRNA和蛋白水平明显下降,而抑制miR-148a后DNMT1 mRNA和蛋白水平明显升高,差异均有显著性(P<0.05)。将WtDNMT1 3'UTR荧光素酶报告质粒与miR-148a共转染,荧光素酶活性显著下降(P<0.05);将MutDNMT1 3'UTR荧光素酶报告质粒与miR-148a共转染,荧光素酶活性无明显改变。提示miR-148a可能通过与DNMT1的mRNA 3'UTR碱基UGCACUG发生互补配对结合,从而靶向抑制其表达。过表达miR-148a后,细胞内总胆固醇和游离胆固醇含量明显增加;沉默miR-148a后,细胞内总胆固醇和游离胆固醇含量明显降低(P均<0.05);而细胞内胆固醇酯含量无明显变化。给予100 μmol/L Hcy干预泡沫细胞后,miR-148a甲基化呈显著下降趋势;给予叶酸+维生素B12干预后,miR-148a甲基化水平较Hcy显著升高(P均<0.05)。过表达DNMT1后,miR-148a启动子区DNA甲基化水平升高(P<0.05)。 【结论】 miR-148a是DNMT1的特异性miRNA,其可能通过直接与DNMT1 3'UTR部分碱基序列UGCACUG发生互补配对结合影响DNMT1表达;miR-148a可通过增加泡沫细胞内总胆固醇和游离胆固醇含量参与Hcy致泡沫细胞的形成过程;DNMT1在miR-148a启动子区甲基化过程中发挥重要作用。     

miR-19a对腹主动脉瘤血管平滑肌细胞增殖迁移及炎症浸润的影响

目的：探讨miR-19a对腹主动脉瘤（AAA）血管平滑肌细胞增殖迁移及炎症浸润的影响及其机制。方法：收集AAA发生后未行任何治疗的15例患者血清，15例同期健康体检者血清，以及15例手术切除AAA患者的腹主动脉瘤组织和15例正常腹主动脉组织。RT-qPCR检测miR-19a的表达水平；CCK-8检测血管平滑肌细胞（VSMCs）增殖活力；Transwell检测VSMCs迁移能力；流式细胞术检测VSMCs凋亡水平；ELISA法检测炎症因子的表达水平；Western blot检测蛋白的表达水平；双荧光素酶报告基因验证miR-19a和CDKN2B的靶向关系。结果：与对照组比，miR-19a在AAA患者组织和血清中均明显高表达。敲降miR-19a可明显抑制VSMCs增殖、迁移并诱导细胞凋亡，下调THP-1细胞炎症因子和基质金属蛋白酶（MMPs）的表达水平。双荧光素酶报告基因结果显示，miR-19a通过结合CDKN2B基因的3’非编码区（3’ UTR），进而抑制其表达水平。过表达CDKN2B可明显缓解miR-19a对VSMCs增殖和转移的抑制作用，以及THP-1细胞的炎症反应。结论：敲降miR-19a可通过抑制VSMCs增殖和迁移，以及下调炎性细胞浸润缓解AAA的发展进程，其机制是通过靶向上调CDKN2B的表达水平。     

DNMT1蛋白通过沉默MEG3基因促进视网膜母细胞瘤增殖

目的 探讨 DNMT1 蛋白是否通过沉默 MEG3 基因诱导视网膜母细胞瘤增殖。方法 通过转染 pcDNA-DNMT1 或si-DNMT1上调或干扰DNMT1的表达水平;通过转染pcDNA-MEG3或si-MEG3上调或干扰MEG3的表达水平;用Western blot检测视网膜母细胞瘤细胞系中DNMT1蛋白表达量;用CCK-8法及EdU法检测细胞的增殖能力;用实时荧光定量PCR技术检测转染后的视网膜母细胞瘤细胞中MEG3表达量的变化;干扰DNMT1表达后,用甲基化特异性PCR检测MEG3基因启动子DNA甲基化水平的变化。结果 视网膜母细胞瘤SO-RB50及HXO-RB44细胞中DNMT1蛋白表达量显著升高（P<0.05）。转染了 pcDNA-DNMT1 的 HXO-RB44 细胞中 DNMT1 蛋白表达增加,细胞增殖能力增加,MEG3 表达量降低;转染了 siRNADNMT1的SO-RB50细胞DNMT1蛋白表达减少,细胞增殖能力降低,MEG3表达量增加（P<0.05）。干扰DNMT1蛋白表达后,MEG3基因启动子DNA甲基化水平降低（P<0.05）。逆转DNMT1蛋白对MEG3基因的调控后,DNMT1蛋白调控RB细胞增殖的能力减弱（P<0.05）。结论 在视网膜母细胞瘤细胞中,DNMT1蛋白表达的上调,诱导了MEG3基因启动子DNA甲基化失活,最终导致细胞异常增殖。     

miR-34a靶向调控NOTCH1基因对SW480细胞增殖的影响

目的 探讨miR-34a靶向调控NOTCH1基因表达而对结肠癌SW480细胞增殖的影响.方法 通过生物信息学预测,NOTCH1为miR-34a特异性靶基因.构建含miR-34a结合位点的NOTCH1基因3 '-UTR域荧光素酶报告载体.通过荧光素酶报告载体系统检测miR-34a与NOTCH1的3’-UTR相互作用对荧光素酶活性的影响;免疫印迹技术检测miR-34a对NOTCH1蛋白表达的影响.采用MTT法及流式细胞检测转染miR-34a对SW480细胞增殖的影响.结果 经过酶切及基因测序鉴定,NOTCH1基因3'-UTR序列的双荧光素酶报告重组质粒构建成功;荧光素酶结果显示在SW480细胞中加入miR-34a的类似物和重组载体,荧光素酶的活性是只加入空载体的SW480组53.4％ (P=0.003 8);而在HEK293细胞中加入miR-34a的抑制物和重组载体,荧光素酶的活性是只加入空载体的HEK293组145％(P=0.002 1),说明miR-34a有与NOTCH1的3’-UTR位点相结合.免疫印迹结果显示在SW480细胞中加入miR-34a的类似物,NOTCH1蛋白的表达水平是未处理SW480组下降53.6％(P＜0.05);而在HEK293细胞中加入miR-34a的抑制物,NOTCH1蛋白的表达水平较未处理HEK293组升高78.9％ (P =0.03),说明miR-34a负性调控NOTCHl蛋白的表达.miR-34a过表达的SW480细胞较未处理的SW480的生长速度明显减慢(P＜0.05),且阻滞在G0～G1期,说明miR-34a过表达后能抑制SW480细胞增殖.结论 miR-34a负性靶向调控NOTCH1基因的表达而抑制SW480细胞的增殖.     

miR-106a对胶质瘤增殖和凋亡的影响及机制研究

目的 探讨微小RNA（miR）-106a对人胶质瘤细胞中腺瘤样息肉（APC）基因的靶向调控作用以及对细胞增殖和凋亡的影响。方法 收集2020年1月至2021年12月浙江省立同德医院减压切除的10例胶质瘤组织和10例对照组织。采用实时荧光定量PCR法检测miR-106a在胶质瘤组织和对照脑组织中、在胶质瘤LN229、U251和U87等细胞系中的表达水平并作比较。使用miR-106a抑制物转染LN229和U251细胞,采用克隆形成实验检测细胞增殖能力,通过流式细胞术检测细胞凋亡水平。通过TOP/FOP荧光素酶实验检测荧光素酶活性,采用Western blot法检测Wnt通路相关蛋白和核内β-连环蛋白（β-catenin）的表达。采用qRT-PCR和Western blot法检测APC的mRNA和蛋白表达水平。利用双荧光素酶报告基因实验确认miR-106a和APC的靶向关系。通过表型拯救实验检测miR-106a靶向APC对胶质瘤细胞增殖和凋亡的影响。结果 miR-106a在胶质瘤组织和细胞系中高表达。抑制miR-106a表达降低胶质瘤细胞的增殖水平,提高胶质瘤细胞的凋亡水平,并抑制了Wnt/...     

乙肝病毒X蛋白通过DNA甲基化沉默肝癌中miR-338表达

目的 将HBx相关的DNA甲基化和非编码RNA异常变化联系起来, 研究二者是否存在相互作用.方法 利用高通量芯片及qRT-PCR证实HBx在肝癌细胞和组织内下调miR-338表达.通过系列蛋白/mRNA/表达分析, 启动子甲基化检测来研究HBx下调miR-338的机制.结果 芯片及qRT-PCR显示HBx在肝癌细胞和组织内下调miR-338的表达.HBx在HepG2肝癌细胞内显著上调DNMT 1和DNMT 3A的表达.HBV阳性肝癌组织中miR-338启动子甲基化程度显著高于癌旁正常组织, 去甲基化处理能挽救肝癌细胞中miR-338表达.结论HBx在体内外显著抑制肝癌中miR-338表达并上调肝癌细胞中DNMT 1和DNMT 3A表达.miR-338启动子过甲基化是其沉默的内在机制.     

miR-26a在动脉粥样硬化发生中的作用

目的 MicroRNAs通过调节血管平滑肌细胞(VSMCs)表型转化、增殖、迁移来影响动脉粥样硬化斑块形成,本文探讨miR-26a在动脉粥样硬化发生过程中的作用。方法采用终浓度为50 mg/L的氧化型低密度脂蛋白(ox LDL)诱导VSMCs构建动脉粥样硬化模型;采用real-time PCR检测VSMCs中miR-26a表达;运用western blot检测平滑肌α-肌动蛋白(SMα-actin)和平滑肌肌球蛋白重链(MYH11)蛋白表达;流式细胞仪、Brd U和transwell迁移实验检测VSMCs凋亡、增殖和迁移能力变化。结果 ox LDL诱导VSMCs中miR-26a表达显著升高(3.22±0.21 vs 1.03±0.03,t=10.56,P<0.001),anti-miR-26a转染VSMCs能显著减少ox LDL诱导的miR-26a表达(P<0.05)。功能学实验发现ox LDL显著下调VSMCs分化标志物SMα-actin和MYH11蛋白表达、抑制细胞凋亡、促进细胞增殖和迁移(P<0.05),而anti-miR-26a能够逆转ox LDL对VSMCs的作用(P<0.05)。结论 oxLDL处理的VSMCs中miR-26a异常升高,miR-26a促进VSMCs增殖和迁移并抑制细胞凋亡和分化,提示miR-26a在动脉粥样硬化过程中可能发挥关键作用。     

miR-30b在高磷诱导的血管平滑肌细胞凋亡中的作用

目的 观察微小RNA30b(microRNA 30b, miR-30b)在高磷诱导的血管平滑肌细胞(vascular smooth muscle cells, VSMCs)凋亡中的作用及机制。方法 体外培养大鼠胸主动脉VSMCs,分为正常组和高磷组(给予10 mmol/Lβ-甘油磷酸盐刺激)。采用实时荧光定量PCR检测各组VSMCs miR-30b表达、Western blot法测定促凋亡基因BAX、抑凋亡基因Bcl-2的表达情况,采用MTT法检测各组细胞的增殖情况、采用流式细胞法检测各组细胞的凋亡情况。为进一步验证miR-30b对VSMCs凋亡的影响,给与转染miR-30b的抑制物inhibitor-30b和类似物mimic-30b,观察VSMCs增殖和凋亡情况以及BAX、Bcl-2表达情况。结果 (1)高磷对VSMCs凋亡的影响：流式结果显示,高磷组VSMCs凋亡细胞显著增多(P<0.05);MTT结果显示,高磷组细胞增殖减少(P<0.05);高磷组BAX表达升高、Bcl-2表达降低(P<0.05);高磷组miR-30b的表达显著下降(P<0.05)。(2)...     

2022miR-216a、miR-301a调控BMPR2促进缺氧环境下肺动脉平滑肌细胞的增殖

目的: 探讨miR-216a、miR-301a对缺氧环境下人肺动脉平滑肌细胞（human pulmonary arterial smooth muscle cells,HPASMC）增殖的影响并研究其发生机制。方法: 采用CCK8法检测常氧和不同缺氧时间培养下HPASMC的增殖活力,qRT-PCR检测各组miR-216a、miR-301a和骨形成蛋白2型受体（bone morphogenetic protein receptor type 2,BMPR2）mRNA表达。CCK8和蛋白免疫印迹法分别检测转染了miR-216a或miR-301a抑制物后HPASMC的增殖能力及增殖细胞核抗原（proliferating cell nuclear antigen,PCNA）表达水平。双荧光素酶报告分别检测BMPR2与miR-216a、BMPR2与miR-301a之间的关系。CCK8和蛋白免疫印迹法分别检测同时转染miR-216a或miR-301a联合BMPR2过表达质粒后HPASMC的增殖能力及BMPR2、PCNA的表达水平。结果: 随着缺氧时间的延长,HPASMC的增殖活力和miR-216a表达增加,BMPR2 mRNA表达逐渐下降,miR-301a在缺氧24 h表达水平最高。下调miR-216a和miR-301a均显著抑制HPASMC的增殖活力及PCNA表达。双荧光素酶报告测定证实BMPR2分别是miR-216a和miR-301a的靶点。同时转染miR-216a或miR-301a联合BMPR2过表达质粒,BMPR2表达下降,细胞增殖活力及PCNA表达增加。结论: 缺氧环境下,miR-216a、miR-301a通过抑制BMPR2促进HPASMC增殖,引发肺动脉高压的形成。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.