肝癌患者血小板4项参数的分析探讨

目的：探讨肝癌患者与正常健康人及化疗前后血小板4项参数的变化和临床意义。方法：用Sysmex，SE-9000全自动血液分析仪对正常健康人、肝癌患者及化疗前后血小板数（PLT）、血小板平均体积（MPV）、血小板分布宽度（PDW）、大血小板比率（P—LCR）4项参数进行检测并比较。结果：肝癌患者PLT结果低于正常对照组，MPV结果高于正常对照组（P〈0．05），差异有统计学意义。PDW、P—LCR结果明显高于正常对照组（P〈0．01），差异有统计学意义。肝癌患者化疗后PLT、MPV、PDW、P-LCR结果均明显低于化疗前（P〈0．01），差异有统计学意义。结论：血小板参数的检测可作为肝脏受损程度，肝癌疗效及预后判断的参考指标。     

基于血脂代谢紊乱理论研究血管软化丸抗动脉粥样硬化的作用机制

目的观察血管软化丸对载脂蛋白E基因敲除(ApoE-/-)小鼠血脂、血液流变学指标、血小板活化标志物CD63表达、主动脉粥样斑块病理形态及病灶处核转录因子-κB(NF-κB)的影响。方法采用高脂饲料喂饲ApoE-/-小鼠,60只ApoE-/-小鼠随机分为4组,正常对照组喂普通饲料,其他各组喂养饲高脂饲料建立动脉粥样硬化模型,各组按规定连续灌胃8周后测定血清血脂水平[总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)],血液流变学指标(全血黏度、血浆黏度、红细胞聚集指数)、血小板活化标志物CD63表达、主动脉粥样斑块病理形态及病灶处NF-κB的含量。结果高脂饮食引起小鼠血脂及血液流变学指标显著升高,导致ApoE-/-小鼠主动脉粥样斑块形成。与模型组比较,血管软化丸组TC、TG、LDL-C水平降低(P 0.05),HDL-C升高(P 0.05);全血低、中、高切黏度、血浆黏度、红细胞聚集指数降低(P 0.05)。各组小鼠主动脉斑块出现不同程度的染色强阳性,中药组平均光密度值较辛伐他汀组明显减小(P 0.05)。结论血管软化丸通过调控高脂饮食引起的血脂代谢紊乱及异常血液流变学指标,降低血小板活化标志物CD63表达和周围血管壁NF-κB含量,起到抗动脉粥样硬化的作用。     

氟伐他汀对家兔颈动脉粥样斑块巨噬细胞和基质金属蛋白酶的影响

目的观察高胆固醇血症家兔颈动脉粥样斑块内炎性巨噬细胞与MMP-2、MMP-9的表达情况，并研究氟伐他汀干预对巨噬细胞聚集和基质金属蛋白酶（MMPs）表达的影响，探索他汀类药物在稳定颈动脉斑块中的作用及机制。方法24只家兔随机分为对照组、高脂组和治疗组，每组8只，分别给予普通饲料、高脂饲料和高脂饲料加氟伐他汀喂养，测定不同时间点血清总胆固醇（TC）、三酰甘油（TG）、低密度脂蛋白胆固醇（LDL-C）和高密度脂蛋白胆固醇（HDL-C）水平。喂养第12月时处死动物，取颈动脉进行石蜡切片，测量并计算颈动脉I／M比值，SP法进行CD68、MMP-2和MMP-9免疫组织化学染色。结果治疗组血清TC、LDL-C浓度明显低于高脂组（P〈0．01）。治疗组I／M比值明显低于高脂组（P〈0．01）。高脂组颈动脉斑块中见大量CD68阳性细胞，而治疗组CD68阳性细胞数显著少于高脂组（P〈0．01）。颈动脉斑块中MMP-2、MMP-9阳性细胞和染色强度明显增加，治疗组MMP-2和MMP-9的表达较高脂组显著减少（P=0．002和P=0．016）。结论氟伐他汀治疗可以抑制高胆固醇血症家兔颈动脉粥样斑块的形成．减轻斑块内巨噬细胞的浸润并抑制MMP-2和MMP-9的产生，从而起到稳定斑块的作用。     

早期联合检测血小板膜糖蛋白及血小板参数对脓毒症患者的临床意义

目的:探讨早期联合检测血小板膜糖蛋白表达水平及血小板参数对脓毒血症患者的临床意义。方法:选取40例脓毒症患者,根据急性生理与慢性健康状况(APACHE)Ⅱ评分分为1组(10分)、2组(10~19分)和3组(20分),所有患者均在确诊24 h内采用全自动血细胞仪检测血小板计数(PLT)、大血小板比率(P-LCR)、血小板平均体积(MPV)、血小板分布宽度(PDW);采用流式细胞仪检测血小板膜糖蛋白CD62P、CD63的表达水平。结果:1随着APACHEⅡ评分升高,患者PLT显著下降,P-LCR、MPV、PDW均显著上升(P0.05);2随着APACHEⅡ评分升高,CD62P、CD63表达均上调(P0.05);3Pearson相关性分析显示:随着PLT的下降,血小板MPV、PDW、P-LCR均逐渐上升,血小板MPV、PDW、P-LCR与PLT呈高度负相关性(r=-0.442、-0.395、-0.472,P0.01),PLT与APACHEⅡ评分呈高度负相关(r=-0.602,P0.01);血小板膜糖蛋白CD62P、CD63表达水平与APACHEⅡ评分呈高度正相关(r=0.603、0.619,P0.01);血小板MPV与CD62P、CD63表达水平均呈正相关(r=0.382、0.310,P0.05),血小板P-LCR、PLT、PDW与CD62P、CD63表达水平之间无明显相关性(P0.05)。结论:脓毒症患者早期体内存在血小板高激活状态,根据血小板膜糖蛋白CD62P、CD63及血小板参数可初步判定脓毒症患者病情的变化。     

无症状性脑梗死患者血小板参数及功能的变化

目的 观察无症状性脑梗死(SCI)患者血小板参数及功能的变化.方法 SCI和正常对照组各47例,用全自动血细胞分析仪检测血小板计数(PLT)、平均血小板体积(MPV)、血小板分布宽度(PDW)、大血小板比率(P-LCR)的变化;用不同诱导剂测定血小板最大聚集率;血浆血栓烷B2(TXB2)和(6-keto-PGF1α)的水平用ELISA法测定.结果 SCI患者的MPV、PDW、P-LCR均高于正常对照组,且差异显著(P＜0.001);两组PLT差异无显著性(P＞0.05);SCI患者血小板最大聚集率均显著高于正常对照组(P＜0.001);SCI组TXB2水平显著高于正常对照组(P＜0.001),而6-keto-PGF1α水平则显著低于正常对照组(P＜0.001).结论 血小板参数的改变及血小板功能亢进是SCI发生的危险因素,血小板参数及功能的测定,对于预防SCI发展为有郑状性脑梗死具有重要的参考价值.     

肝炎肝硬化和重型肝炎患者血小板4项指标的测定意义

研究肝病患者血小板功能与肝病严重程度之间关系,采用日本东亚SysmexF-800型血细胞自动计数仪检测了150例肝炎肝硬化（LC）、150例重型肝炎、114例急性黄疸型肝炎、107例慢性肝炎和50例健康成人的血小板功能4项指标：血小板计数（PLT）、平均血小板体积（MPV）、血小板压积（PCT）^-和血小板分布宽度（PDW）。结果显示：肝炎肝硬化组和重型肝炎组患者的PLT和PCT值均显著降低（P〈0．05或P〈0．01）,特别是两组中的明显出血者降低更显著。提示血小板的4项指标除问接反映血小板的功能外,对评估病毒性肝炎的严重程度、出血倾向有重要的辅助指导意义。     

回心草对兔动脉粥样硬化和缺血心肌血管新生作用的研究

目的：建立兔实验性动脉粥样硬化和心肌梗死双模型,观察回心草提取液对兔动脉粥样硬化斑块和缺血心肌血管新生的影响。方法选择30只家兔,随机分为3组,普通饲料对照组（对照组）,高脂饲料组（高脂组）,回心草干预组（干预组）,每组各10只,共喂养9周。第9周末,采用开胸结扎兔冠状动脉左前降支（LAD）的方法,建立兔急性心肌梗死模型。实验终点时,测定血液生化指标,对血管及心脏组织行病理学检查,免疫组化染色测定心肌组织分化抗原簇34（CD34）及血管内皮细胞生长因子受体2（VEGFR2）阳性反应强度。结果3组比较,高脂组的肌酸激酶同工酶（CK-MB）、肌钙蛋白I（TnI）、总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）明显升高,差异具有统计学意义（P＜0.01）;干预组与高脂组比较,TC、TG、CK-MB、TnI明显下降（P＜0.01）, LDL-C下降（P＜0.05）。高脂组与对照组病理评分比较,差异有统计学意义（P＜0.01）;干预组的病理评分较对照组高,较高脂组低（P＜0.05）。高脂组心肌组织CD34和VEGFR2阳性组织相对灰度值均明显高于对照组（P＜0.01）;干预组VEGFR2及CD34表达水平较对照组高、较高脂组低（P＜0.05）。结论高脂饲料喂养和开胸结扎兔冠状动脉LAD的方法可成功建立家兔动脉粥样硬化和急性心肌梗死双模型。回心草提取液减轻了动脉粥样硬化和心肌缺血坏死,抑制了心肌组织CD34和VEGFR2的表达。     

氨氯地平干预兔动脉粥样硬化过氧化脂质代谢、前列环素/血栓烷A2平衡异常的研究

目的 :观察动脉粥样硬化 （AS）兔过氧化脂质代谢、前列环素 （PGI2 ） /血栓烷A2 （TXA2 ）平衡异常以及氨氯地平干预作用。方法 :采用高脂饲料或高脂饲料加氨氯地平喂养家兔 6 0d ,喂养前、后抽血测生化指标 ,喂养后处死动物作组织学研究。结果 :喂养后血清总胆固醇 （TC）、主动脉和血浆丙二醛 （MDA）、全血血小板活化因子 （PAF）、血浆TXB2 、内皮素 （ET）水平显著升高 ,血小板聚集性增强 ,而主动脉组织和血浆 6 酮 前列腺素 （6 Keto PGF1α）、血浆超氧化物歧化酶 （SOD）水平显著下降 ,并且发生AS病变。氨氯地平可以降低MDA及ET含量 ,提高PGI2 /TXA2 比值 ,减轻AS病变。结论 :AS家兔体内出现脂质过氧化代谢异常、PGI2 /TXA2 平衡失调 ,而氨氯地平具有明显的拮抗作用 ,其机制可能与抗氧化有关。     

三七总甙对糖尿病肾病大鼠血栓素B2及6-酮-前列腺素F1α的影响

[目的]通过观察三七总甙(PNS)对糖尿病肾病大鼠血栓素B2(TXB2)、6-酮前列腺素F1α(6-ketoPGF1α)的影响,探讨PNS防治糖尿病肾病的作用及机制.[方法]采用高脂饲料喂养加尾静脉注射链脲佐菌素(STZ),建立2型糖尿病肾病大鼠模型,PNS低、高剂量组分别予PNS 100、200 mg/(kg·d)灌胃,12周后,测定空腹血糖(FBG)、血脂、24 h尿微量清蛋白、TXB2、TXB2/6-keto-PGF1α.[结果]糖尿病组大鼠FBG、24 h尿微量清蛋白、TXB2、TXB2/6-keto-PGF1α明显升高,PNS组以上各项指标均明显降低.[结论]PNS能有效防治糖尿病肾病的作用可能与降低TXB2、TXB2/6-keto-PGF1α有关.     

氨氯地平对老年高血压患者血小板活性的影响

目的 探讨老年高血压与血小板活性的关系，钙拮抗剂对血小板活性的影响及其在高血压病治疗中的意义。方法 采用流式细胞仪检测老年高血压患者氨氯地平治疗前后与对照组的血小板胞浆内[Ca^2 ]，与采用放射免疫法测定血浆TXB2和6-kPGF1α。结果 高血压组血小板胞浆内[Ca^2 ],血浆TXB2明显高于正常对照组。血浆6-k-PGF1α明显低于正常对照组（P均＜0．001）。高血压组用药后血小板胞浆内[Ca^2 ]血浆TXB2明显低于用药前，6－k-PGF1α明显高于用药前（P均＜0．001）。结论 老年高血压患者血小板活性增强，用钙离子拮抗剂氨氯地平治疗后可显著降低血小板活性，防治高血压心脑血管并发症具有重要意义。     

初治涂阳肺结核患者血小板相关指标研究

目的探讨血小板相关指标在初治涂阳肺结核(SPPTB)诊治中的意义。方法分别检测234例初治SPPTB患者治疗2月前后和39例健康对照组的血小板计数(PLT)、血小板平均体积(MPV)、血小板分布宽度(PDW)、大血小板比率(P-LCR)和血小板压积(PCT)5项血小板相关指标。分析指标的变化情况及其与痰涂片结果的关联性。结果初治SPPTB患者PLT、PCT较健康对照组均明显升高(均P=0.00),MPV、PDW、P-LCR较健康对照组均明显降低(均P=0.00);2月末痰涂片转阴组和2月末痰涂片未转阴组5项指标比较,差异均无统计学意义(均P0.05)。与治疗前比较,2月末痰涂片转阴组治疗后PLT和PCT均显著降低(均P=0.00),MPV、PDW和P-LCR差异均无统计学意义(均P0.05);2月末痰涂片末转阴组治疗后5项指标差异均无统计学意义(均P0.05)。初治SPPTB患者初次痰涂片抗酸杆菌阳性级别与PLT、MPV、PDW、P-LCR、PCT 5项指标均无相关性(均P0.05)。结论 PLT和PCT可作为初治SPPTB患者诊断和疗效评估的参考指标。     

血小板参数和凝血指标与溶栓治疗关系的探讨

目的 动态观察急性脑梗死患者溶栓治疗过程中血小板参数及凝血指标的变化规律,探讨血小板参数和凝血指标与溶栓治疗的关系.方法 应用血细胞分析仪检测180例急性脑梗死患者（实验组）和180例健康体检者（对照组）的血小板计数（PLT）、血小板平均体积（MPV）和大血小板比率（P-LCR）,血凝分析仪检测凝血酶原时间（PT）、部分活化凝血活酶时间（APTT）、纤维蛋白原（Fg）及凝血酶时间（TT）,动态观察溶栓前及溶栓后1、2、4小时和48小时血小板参数和凝血指标的变化.结果 溶栓前脑梗死患者的MPV、P-LCR和Fg含量均高于对照组（P均＜0.05）,PLT、PT和APTT显著低于对照组（P均＜0.05）;溶栓治疗后,MPV、P-LCR和Fg含量显著下降,PLT、PT和APTT显著升高（P均＜0.05）.结论 溶栓治疗过程中,动态监测急性脑梗死患者的血小板参数（PLT、MPV和P-LCR）和凝血指标（PT、APTT、Fg）,可反映患者凝血功能的变化,对脑梗死患者的溶栓治疗具有一定的参考价值.     

血小板参数和凝血指标的变化与肝硬化Child-Pugh分级的关系

目的了解肝硬化患者血小板参数和凝血指标的变化及其与肝功能Child-Pugh分级的关系。方法分别采用全自动血液分析仪和全自动血凝仪测定148例肝硬化患者和50例健康对照者的血小板参数(PLT、MPV、PDW、P-LCR)和凝血功能指标(PT、aPTT、TT、Fbg),比较肝硬化组与对照组、肝硬化Child-Pugh分级后组间及出血组与未出血组间血小板参数和凝血指标的变化。结果与对照组比较,肝硬化患者PLT下降,MPV、PDW、P-LCR升高,Fbg降低,PT、aPTT、TT延长,差异有统计学意义(P<0.01);随着Child-Pugh等级上升,其PLT逐渐下降,MPV、PDW、P-LCR逐渐升高,Fbg逐渐降低,PT、aPTT、TT逐渐延长(P<0.01);出血组与未出血组的血小板参数和凝血指标差异均有统计学意义(P<0.01)。结论肝硬化患者存在血小板及凝血功能异常,其血小板参数和凝血指标的变化与肝功能Child-Pugh分级和出血与否密切相关,是判定肝硬化损害程度及出血倾向的重要指标。     

Factors associated with granulocyte colony-stimulating factor-induced peripheral blood stem cell yield in healthy donors

BACKGROUND AND OBJECTIVES: Poor collection results are a clinical problem in granulocyte-colony stimulating factor (G-CSF)-induced peripheral blood stem cell (PBSC) collection in healthy donors. It would be beneficial to be able to predict the PBSC yield from allogeneic donors before mobilization or harvesting. MATERIALS AND METHODS: We examined the relationship between certain donor characteristics and the effectiveness of G-CSF-induced PBSC collection in 59 healthy family donors aged 3-63 years old (median 16 years). G-CSF was administered subcutaneously at 10 microg/kg for mobilization, daily for 5 days, and PBSC harvest using a continuous blood cell separator was started on day 5 of G-CSF treatment. Total cell yields were calculated as the number per unit of processed blood (l) per unit weight of the donor (kg). RESULTS: In a univariate analysis, the donor's age, body mass index (BMI), white blood cell (WBC) count before mobilization, and platelet count before and during mobilization were significantly correlated with the yield of mononuclear cells (MNC), CD34(+) cells and granulocyte-macrophage colony-forming units (GM-CFU). Younger age (P < 0.001), a low BMI (P = 0.002), a high WBC count before mobilization (P = 0.004), a high platelet count before (P = 0.012) and during (P < 0.05) mobilization, and a low speed of withdrawal (P = 0.019) were associated with a higher CD34(+) cell yield. No significant correlation was found for gender, the type of G-CSF, the serum level of G-CSF, the type of cell separator, or the type of blood access. A multivariate forward and backward stepwise selection regression analysis showed that the factors associated with CD34(+) cell yield were age, platelet count before and during mobilization, and circulating CD34(+) cell concentration on day 2 of G-CSF treatment. CONCLUSION: In this small preliminary study, we found that donor age is the most important factor in predicting G-CSF-induced PBSC yields. Old age and low platelet counts before mobilization might be useful indicators for identifying poor mobilizers. Further validation of these findings in a larger number of donors are needed to establish whether these findings apply to other populations.     

Peripheral blood stem cell mobilization by granulocyte colony-stimulating factor alone and engraftment kinetics following autologous transplantation in children and adolescents with solid tumor

In 56 pediatric and adolescent patients (median age 7 years, range 1-21) with various solid tumors, peripheral blood stem cells (PBSC) were mobilized with granulocyte colony-stimulating factor (G-CSF) alone, and the yields of PBSC and engraftment kinetics following autologous peripheral blood stem cell transplantation (PBSCT) were evaluated retrospectively. Granulocyte colony-stimulating factor (10 microg/kg) was injected subcutaneously for mobilization when patients showed no influence of previous chemotherapy, and administration was continued for 5 days. The peaks of CD34+ cells and colony-forming units-granulocyte/macrophage in the blood were observed on days 4 through 6 of G-CSF administration in all patients. Peripheral blood stem cell harvest was commenced on day 5 of G-CSF treatment. Compared to the results in patients mobilized by chemotherapy plus G-CSF (N=18), the progenitor cell yields were lower in patients mobilized with G-CSF alone. However, there were no significant differences in WBC and ANC engraftment compared to the chemotherapy plus G-CSF mobilization group. Platelet recovery following autologous PBSCT was delayed in patients mobilized with G-CSF alone. The median time taken for ANC and platelet counts to reach 0.5 x 10(9) and 20 x 10(9)/l was 12 days (range: 9-28) and 15 days (8-55), respectively, in all patients who received PBSC mobilized by G-CSF alone. In summary, mobilization with G-CSF alone can mobilize sufficient CD34+ cells for successful autografting and sustained hematological reconstitution in pediatric and adolescent patients with solid tumors, and even in heavily pre-treated patients.     

Platelet activation and platelet–leukocyte interaction in β-thalassemia/hemoglobin E patients with marked nucleated erythrocytosis

Patients with thalassemia, an inherited hemolytic anemia, have increased risk of hypercoagulable complications. A whole blood flow cytometric (FCM) method has been used for studies of platelet activation and platelet–leukocyte aggregation in these patients. However, this FCM method presents technical difficulties because of the high proportion of immature red blood cells (RBCs) in these patients. A protocol for the simultaneous measurement of platelet activation and their aggregation with leukocyte populations in whole blood using four-color FCM which excluded immature RBC was devised, and evaluated for the evaluation of platelet function in patients with β-thalassemia/hemoglobin E (HbE). Whole blood from these patients and from healthy volunteers was stained for platelet activation and platelet–leukocyte aggregates using anti-CD42a, anti-CD62P, anti-CD45 and glycophorin A (GPA) conjugated with different fluorochromes. Our FCM method is simple, effective and based on the assumption that GPA is present on all immature RBCs, but is not expressed on CD45⁺ leukocytes. Results from the studies showed that blood samples from these patients contained a high frequency of circulating activated platelets (CD42a⁺/CD62P⁺) when compared to samples from healthy individuals. The percentage of platelet–neutrophil, platelet–monocyte—but not platelet–lymphocyte—aggregates were also elevated in both thalassemia genotypes with marked increase in patients who had undergone splenectomy. These findings suggest that platelets adhere to neutrophils and monocytes are activated which support the clinical observation that splenectomized thalassemia patients have an increased risk of arterial or venous thrombotic manifestations.     

慢性乙型肝炎患者凝血功能和血小板参数检测结果分析

目的探索慢性乙型肝炎患者凝血功能和血小板参数变化的临床意义。方法收集慢性肝炎重度42例、慢加急肝衰竭24例、慢性肝衰竭35例、肝硬化32例和健康人群50例,检测血小板参数（PLT、MPV、PDW、P-LCR）和凝血功能指标（PT、APTT、TT、Fig）。结果与对照组比,各型慢性肝炎组MPV、PDW、P-LCR升高,PLT降低（P〈0.01）;慢加急肝衰竭、慢性肝衰竭和肝硬化组MPV、PDW、P-LCR高于慢性肝炎重度组,PLT低于慢性肝炎重度组（P〈0.01）;慢加急肝衰竭组PLT、MPV、PDW和慢性肝衰竭组MPV高于肝硬化组（P〈0.05,P〈0.01）;慢加急肝衰竭组与慢性肝衰竭组PLT差异有统计学意义（P〈0.01）;与对照组比较,各型慢性肝炎组PT、APTT、TT升高,Fig降低（P〈0.01）;慢加急肝衰竭组、慢性肝衰竭组、肝硬化组PT、APTT、TT高于慢性肝炎重度组（P〈0.01）,Fig低于慢性肝炎重度组（P〈0.01）;慢加急肝衰竭组和慢性肝衰竭组Fig低于肝硬化组,慢性肝衰竭组APTT高于肝硬化组（P〈0.01）;慢加急肝衰竭组与慢性肝衰竭组APTT差异有非常显著性意义（P〈0.01）。结论凝血功能和血小板参数反映了患者肝脏损害程度和出血倾向。     

Successful peripheral blood stem cell mobilization using pegfilgrastim in allogeneic stem cell transplantation

Pegfilgrastim is produced by binding a 20,000-dalton polyethylene glycol molecule to granulocyte colony-stimulating factor (G-CSF), increasing the mass of the compound, and resulting in a longer-lasting form of G-CSF. This makes it more convenient to use pegfilgrastim as a single-day injection. This study was a prospective phase II single-center trial. Fifteen normal related donors received pegfilgrastim 12 mg subcutaneously to mobilize peripheral blood stem cells (PBSC) for allogeneic stem cell transplantation. Leukapheresis was planned to start 3 days after injection. All harvests were successful. Median number of leukapheresis was 2 days (range 1–3 days). There were 7/15 donors who only required single leukapheresis. The maximum concentration of white blood cells (WBC) and circulating CD34 cells occurred 3 days after pegfilgrastim injection (WBC: median 62,200/μl; CD34: median 69.76/μl). The median yield of CD34 cells was 6.78 × 10⁶/kg recipient weight. The median CD3 cells was 1.89 × 10⁸/kg recipient weight. The main adverse events were bone pain and headache. Median neutrophil and platelet engraftments in the recipients occurred on day 12 and day 13, respectively, after transplantation. PBSC mobilization with single-day injection of pegfilgrastim in normal donor is feasible. Further comparisons of this protocol to standard G-CSF for allogeneic stem cell mobilization should be conducted in future.     

健康体检者血小板参数、P-选择素、PAC-1测定及与年龄的相关性分析

目的 探讨增龄对血小板参数及其活化指标的影响和意义.方法 选择同期行健康体检者400例,其中年龄25～44岁156例（成年组）、45～59岁144例（老年前组）、〉60岁（老年组）100例,均用全自动细胞分析仪测定血小板参数包括血小板计数（PLT）、平均血小板体积（MPV）、血小板体积分布宽度（PDW）和血小板压积（PCT）水平,三组各随机选择30例采用流式细胞仪检测P-选择素（CD62P）及血小板膜糖蛋白Ⅱb/Ⅲa（PAC-1）水平,对年龄与上述指标间相关性行Pearson相关分析.结果 随年龄增加PLT、PCT、PDW下降,MPV增大,CD62P、PAC-1增多;PLT与年龄呈明显负相关,MPV、PAC-1、CD62P与年龄呈明显正相关.结论 血小板参数及其活化指标均与年龄有明显相关性,此为血栓性疾病的预防及诊治提供了理论依据.