支气管哮喘气道炎症表型与Th1/Th2和IgE的相关研究

目的 分析不同气道炎症表型的支气管哮喘患者的循环血辅助性T细胞1/2(Th1/Th2)和IgE水平与临床特征的关系。方法 选择2017年6月至2018年12月于该院诊断为支气管哮喘患者共180例,根据诱导痰液中性粒细胞(Neu)和嗜酸粒细胞(Eos)百分比分为Neu组、Eos组、混合组和寡细胞组,检测循环血Th1/Th2和IgE水平,Th1细胞分泌细胞因子血清干扰素-γ(IFN-)γ和Th2细胞分泌白细胞介素-4(IL-4),比较各组患者的临床特征。结果 Eos组和混合组的Th1/Th2(0.56±0.12、0.59±0.15)明显低于Neu组(0.77±0.23)和寡细胞组(0.72±0.19),但IgE水平[(12.3±4.5)、(11.9±4.3)mg/L],比Neu组和寡细胞组[(7.6±3.2)、(7.2±3.1)mg/L]增加,IFN-γ[(32.6±9.5)、(30.5±8.6)mg/L比(21.2±6.8)、(20.9±5.7)mg/L]和IL-4[(25.7±6.8)、(23.3±6.5)mg/L比(12.4±4.3)、(11.5±4.2)mg/L]水平升高,差异有统计学意义(P<0.05)。Neu组和混合组患者哮喘病程和严重程度明显大于其他两组,吸烟和气道感染率增加;Eos组和混合组患者过敏性鼻炎和皮肤点刺试验阳性率高于其他两组,差异有统计学意义(P<0.05)。4种炎症表型组患者的肺功能包括第1秒用力呼气容积(FEV1)及与用力肺活量的比值(FEV1/FVC)、用力呼气中段流量(MMEF%)、50%用力呼气流量(FEF 50%)和75%用力呼气流量(FEF 75%)比较差异无统计学意义(P>0.05)。结论 支气管哮喘患者具有不同的炎症细胞表型,与循环血Th1/Th2和IgE水平以及临床特征有密切联系。     

Blood MALT1, Th1, and Th17 cells are dysregulated,inter‐correlated,and correlated with disease activity in rheumatoid arthritis patients; meanwhile,MALT1 decline during therapy relates to treatment outcome

ObjectiveMucosa‐associated lymphoid tissue lymphoma translocation protein 1 (MALT1) participates in inflammatory and autoimmune diseases via activating various signaling pathways and promoting the differentiation of T‐helper (Th) 1 and Th17 cells; however, it is rarely reported in rheumatoid arthritis (RA). This study aimed to assess the correlation of MALT1 with Th1 and Th17 cells and evaluate its potential as a biomarker for evaluating disease activity and treatment outcomes in RA patients.MethodsThis study enrolled 139 RA patients and 45 health controls (HCs); then, blood MALT1, Th1, and Th17 cells were determined. For RA patients only, blood MALT1 at week (W) 6 and W12 after treatment was also detected. Additionally, clinical response and remission of RA patients were assessed at W12.ResultsMALT1 (p < 0.001), Th1 (p = 0.011), and Th17 (p < 0.001) cells were all increased in RA patients than HCs; meanwhile, increased MALT1 was associated with elevated Th1 (p = 0.003) and Th17 (p < 0.001) cells in RA patients. Besides, MALT1, Th1, and Th17 cells were positively correlated with parts of disease activity indexes in RA patients (all p < 0.050). In addition, MALT1 was gradually declined from W0 to W12 (p < 0.001) in RA patients. Specifically, MALT1 at W6 and W12 was lower in response patients than no response patients (both p < 0.010), also in remission patients than no remission patients (both p < 0.050).ConclusionMALT1, Th1, and Th17 cells are dysregulated, inter‐correlated, and correlated with disease activity in RA patients; meanwhile, the decline of MALT1 expression can partly reflect RA treatment response and remission.     

Th1/Th2炎性细胞因子在老年肺气肿与肺间质纤维化中的研究

目的 探讨在中国汉族老年人群中,肺气肿和肺间质纤维化患者血Th1/Th2炎性细胞因子的浓度差异趋势.方法 选取肺气肿、肺间质纤维化老年患者和健康人各40例,采集外周静脉血5 ml,采用酶联免疫吸附法检测血清Th1/Th2炎性细胞因子水平,病例组与健康对照组的比较采用独立样本t检验,Th1/Th2炎性细胞因子之间的相关关系采用Pearson直线相关分析.结果 3组平均年龄分别为:健康人组58.26±9.21岁、肺气肿组60.26±10.43岁、肺间质纤维化组61.26±8.83岁,差异均无显著性(P＞0.05).在肺气肿组中干扰素γ(INF-γ)、白介素(IL)-2水平明显升高P ＜0.01,而Th2炎性细胞因子IL-4水平显著降低(P＜0.01),差异具有显著的统计学意义;在肺间质纤维化中,IL-2水平明显降低(P＜0.01),而IL-5、IL-10水平则明显升高(P＜0.05),差异具有统计学意义;同时结果显示细胞因子间存在正相关关系.结论 在老年肺气肿和肺间质纤维化患者Th1/Th2炎性细胞因子有显著的浓度差异变化,其结果为临床治疗提供一个新的视点与思路.     

C型CpG单链寡核苷酸对哮喘小鼠Th1／Th2相关细胞因子的影响

目的C型CpG—ODN对哮喘小鼠Th1／Th2相关细胞因子的影响，为CpG-ODN进一步应用提供依据。方法利用人工合成C型的CpG-ODN（ODN-M362），将其注射于哮喘小鼠腹腔，采用酶联免疫吸附法（ELISA）检测注射后小鼠的Th1和Th2细胞因子的含量，分析其有无差异性。结果C型CpG-ODN组BALF中EOS数量，血清IL-4水平明显低于对照组和哮喘组（P〈0．01），血清IFN-γ明显升高于对照组和哮喘组（P〈0．01）。结论C型的CpG-ODN，对哮喘小鼠Th1／Th2失衡有明显的逆转作用。     

原因不明复发性流产患者外周血PD-1、Th1/Th2型细胞因子水平测定及其相关性研究

目的探讨外周血程序性细胞死亡分子1(PD-1)及血清中Th1/Th2型细胞因子变化在原因不明复发性流产(URSA)发病中的作用。方法采用流式细胞术测定140例URSA患者接受淋巴细胞免疫治疗前后外周血PD-1水平;采用ELISA法测定患者治疗前后血清Th1(IL-2、IFN-γ)/Th2(IL-4、IL-10)型细胞因子水平,同时以102例正常已生育妇女作为对照组。结果 (1)淋巴细胞免疫治疗前与对照组相比,PD-1和Th2型细胞因子(IL-4和IL-10)显著下降(P均<0.05)。(2)淋巴细胞免疫治疗后与治疗前相比,Th1型细胞因子(IL-2和IFN-γ)含量明显下调(P均<0.05)。(3)淋巴细胞免疫治疗后与对照组相比,PD-1、Th1/Th2型细胞因子水平无显著性差别(P均>0.05)。(4)URSA患者外周血PD-1含量和血清中Th2型细胞因子水平呈正相关(r=0.67,r=0.81,P均<0.05);与Th1型细胞因子呈负相关(r=-0.55,r=-0.73,P均<0.05)。(5)淋巴细胞免疫治疗后,妊娠成功的妇女PD-1表达水平显著高于妊娠失败者(P<0.05)。结论 PD-1可能通过下调Th1/Th2型细胞因子的水平抑制URSA的发生发展。     

流式微球分析法检测血清中Th1/Th2/Th17型细胞因子的表达在肺结核病患者中的临床意义

目的探讨流式微球分析法(CBA法)检测血清Th1/Th2/Th17型细胞因子的表达在结核病患者中的临床意义。方法按照BD CBA Flex Set检测试剂盒的要求,用CBA法检测98例肺结核患者(痰涂片阳性肺结核患者38例,痰涂片阴性肺结核患者60例)和79例同期健康体检者的Th1型细胞因子(白细胞介素2、γ干扰素、肿瘤坏死因子),Th2型细胞因子(白细胞介素4、白细胞介素6、白细胞介素10)以及Th17型细胞因子(白细胞介素17A)的表达水平,并分析3组之间的差异。结果除白细胞介素17A组间、组内差异无统计学意义(P0.05)外,结核组白细胞介素2、白细胞介素4、白细胞介素6、白细胞介素10、肿瘤坏死因子、γ干扰素水平均高于对照组(P0.05);痰涂片阳性患者白细胞介素2、白细胞介素4、白细胞介素10、γ干扰素水平均高于对照组(P0.05),痰涂片阳性患者和阴性患者差异无统计学意义(P0.05);而痰涂片阳性患者白细胞介素6和肿瘤坏死因子水平均显著高于痰涂片阴性患者(P0.05)。结论白细胞介素6和肿瘤坏死因子可作为判断结核病严重程度的指标,监测结核病病情的转归。与此同时,CBA法作为1种新兴的检测技术,敏感度高,可同时检测多种细胞因子,并能大大节约血清用量与时间,因此可作为结核病检测和病情监测的实验室技术。     

乙型肝炎外周血Th1/Th2、Tc1/Tc2表达失衡的临床意义

目的探讨Th1/Th2、Tc1/Tc2细胞因子在乙型肝炎中的表达及临床意义。方法采用流式细胞术分析乙型肝炎患者外周血不同T淋巴细胞亚群中细胞因子(IFN-γ、IL-4)的表达,分别比较急慢性乙型肝炎以及慢性轻、中、重度肝炎IFN-γ、IL-4表达水平的变化。结果慢性肝炎Th2、Tc2细胞高于急性肝炎(P<0.01;P<0.05);相对于轻度组,中度Th1、Tc1以及重度组Th1、Tc1均有升高(P<0.01),相对于中度组,重度组Th1、Tc1亦有增高(P<0.05;P<0.01)。结论乙型肝炎外周血Th2、Tc2与炎症慢性化有关;随着慢性乙型肝炎活动程度的加剧Th1、Tc1细胞逐渐增多。     

小青龙汤对哮喘小鼠肺组织Th1/Th2作用的实验研究

目的:观察小青龙汤对哮喘模型小鼠肺组织内Th1/Th2比值的影响并探讨其作用机制.方法:将49只雌性BALB/cA组)、哮喘组(B组)、小青龙汤小剂量组(C组)、小青龙汤大剂量组(D组)、地塞米松组(E组)、必可酮组(F组)及联合用药组(减半的小剂量小青龙汤加地塞米松,G组),每组7只.卵蛋白致敏复制哮喘模型,激发哮喘前1 h,各组小鼠分别使用相应药物治疗.激发12 d后断头处死小鼠,立即取肺脏进行免疫组化染色,观察组织中Th1和Th2表达.结果:①各组哮喘小鼠肺组织内Th1和Th2数量均显著增多(P均<0.01);D组较C组显著减少(P<0.05);D、E、F、G组之间差异均无显著性(P均>0.05).②B组小鼠肺组织内Th1/Th2±<0.01),肺内细胞因子呈明显的Th2表型变化;各治疗组较B组均呈非常显著性升高(P均<0.01),肺内细胞因子呈明显的Th1表型变化;各治疗组间差异均无显著性(P均>0.05),且接近A组.结论:①哮喘小鼠肺组织内Th1/Th2比值明显降低,支持哮喘发病中存在Th1/Th2比值失衡学说.②小青龙汤选择性降低肺组织内Th1、Th2数量,逆转失衡的Th1/Th2比值是其治疗哮喘的重要机制之一.③小青龙汤与糖皮质激素合用有药效相加作用.④小青龙汤逆转Th1/Th2比值的作用与用药时间和用药剂量有关.     

Aberrant expression of long non‐coding RNA PVT1 in allergic rhinitis children: Correlation with disease risk,symptoms, and Th1/Th2 imbalance

BackgroundLong non‐coding RNA plasmacytoma variant translocation 1 (lnc‐PVT1) exacerbates inflammation and induces T helper (Th) 1/Th2 imbalance in allergic diseases, but its clinical role in allergic rhinitis (AR) remains unclear. Hence, we conducted this study to compare lnc‐PVT1 expression among AR children, disease controls (DCs), and health controls (HCs), aiming to investigate its clinical application in AR children.MethodsSixty AR children, 30 DCs, and 30 HCs were enrolled in the study, and then, their lnc‐PVT1 expression in peripheral blood mononuclear cell was detected. Serum interferon‐gamma (IFN‐γ), interleukin 10 (IL‐10), Th1, and Th2 cells in AR children were also analyzed. Besides, lnc‐PVT1 was also detected at Week (W)4 after treatment in AR patients.ResultsLnc‐PVT1 was upregulated in AR children compared with DCs and HCs (both p < 0.001). Lnc‐PVT1 was positively related to nasal rhinorrhea score, itching score, congestion score, and total nasal symptom score (TNSS) in AR children (all p < 0.050), instead of sneezing score (p = 0.115). Lnc‐PVT1 negatively associated with Th1 cells in AR children (p = 0.028) also exhibited a negative correlation trend with IFN‐γ (but without statistical significance) (p = 0.065). Differently, lnc‐PVT1 was positively related to Th2 cells (p = 0.012) and IL‐10 (p = 0.021) in AR children. Besides, lnc‐PVT1 and TNSS were reduced at W4 after treatment in AR children (both p < 0.001); notably, lnc‐PVT1 expression decline was correlated with TNSS decline during treatment (p = 0.013).ConclusionLnc‐PVT1 works as a biomarker, whose aberrant expression is related to disease severity, Th1/Th2 imbalance, and its decrement can reflect treatment outcome in AR children.     

LncRNA GAS5 relates to Th17 cells and serves as a potential biomarker for sepsis inflammation,organ dysfunctions and mortality risk

BackgroundLong noncoding RNA GAS5 (lnc‐GAS5) is able to regulate macrophage M1 polarization and Th17 cell differentiation, also engaged in sepsis‐induced inflammation and organ injury. This study aimed to further evaluate its linkage with Th1 cells and Th17 cells, as well as its clinical value in sepsis management.MethodsAbout 101 sepsis patients were enrolled followed by peripheral blood mononuclear cell (PBMC) and serum samples collection. PBMC lnc‐GAS5 was detected by RT‐qPCR; Th1 cells and Th17 cells in PBMC CD4⁺ T cells were detected by flow cytometry; serum IFN‐γ and IL‐17A were detected by ELISA. Besides, PBMC lnc‐GAS5 was also detected in 50 health controls (HCs).ResultsLnc‐GAS5 was reduced in sepsis patients than in HCs (p < 0.001), which also well‐distinguished sepsis patients from HCs with AUC 0.860. Lnc‐GAS5 did not relate to Th1 cells (p = 0.059) or IFN‐γ (p = 0.192); while negatively linked with Th17 cells (p = 0.002) and IL‐17A (p = 0.019) in sepsis patients. Interestingly, lnc‐GAS5 negatively correlated with SOFA score (p = 0.001), SOFA‐Respiratory system score (p = 0.001), SOFA‐Coagulation score (p = 0.015), and SOFA‐Renal system score (p = 0.026), but not SOFA‐Liver score (p = 0.080), SOFA‐Cardiovascular system score (p = 0.207) or SOFA‐Nervous system score (p = 0.182) in sepsis patients. Furthermore, lnc‐GAS5 was negatively related to CRP (p = 0.002) and APACHE II score (p = 0.004) in sepsis patients. Finally, lnc‐GAS5 was decreased in dead sepsis patients compared to survivors (p = 0.007), which also distinguished sepsis deaths from survivors with AUC 0.713.ConclusionLnc‐GAS5 relates to Th17 cells and serves as a potential biomarker for sepsis severity and mortality risk.     

乳腺良恶性肿瘤患者血清Th1／Th2细胞因子中IL-2特点

目的 分析乳腺良、恶性肿瘤患者血清Th1／Th2细胞因子中IL-2的特点及其意义。方法 用流式荧光免疫微球分析技术（Flow Fluorenscence Immunmicrobeads Amay，FFIA）检测乳腺良、恶性肿瘤患者血清Th1／Th2细胞因子水平。Th1型细胞因子包括IL-2、TNF-α、IFN-γ，Th2型细胞因子包括IL-4、IL-5、IL-10。结果 乳腺良、恶性肿瘤患者血清IL-2水平在检测线以下（IL-2=0）的分别为34．1％（15／44）和42．8％（20／47），明显不同于其他细胞因子。在乳腺良、恶性肿瘤患者中，血清IL-2=0组，IFN-γ、IL-4、IL-5、IL-10水平均相应较低，与血清IL-2〉0组比较差异有统计学意义（P〈0．01），其中TNF-α水平差异无统计学意义（P〉0．05）。乳腺恶性肿瘤中，IL-2水平与肿瘤大小及淋巴结转移状态无相关性。结论 乳腺良、恶性肿瘤患者血清IL-2水平可能影响IFN-γ、IL-4、IL-5、IL-10表达，在乳腺恶性肿瘤中IL-2水平状态与肿瘤大小及淋巴结转移状态无相关性。     

匹多莫德口服液免疫治疗对隐性梅毒患者Th 1／Th2细胞因子的影响

目的：探讨匹多莫德口服液免疫治疗对隐性梅毒患者Th1／Th2细胞因子的影响。方法将隐性梅毒患者60例按随机数字表法分为观察组（30例）和对照组（30例）,对照组给予常规治疗,观察组联合匹多莫德口服液免疫治疗,评估2组临床治疗效果及安全性。结果治疗后3、6个月观察组患者 IL-2、IFN-γ明显高于对照组,2组比较差异具有统计学意义（P＜0．05）。治疗后3、6个月观察组患者 IL-4、IL-10明显低于对照组,2组比较差异具有统计学意义（P＜0．05）。治疗后3、6个月观察组患者有效率明显高于对照组,2组比较差异具有统计学意义（P＜0．05）。结论匹多莫德口服液免疫治疗对隐性梅毒患者Th1／Th2细胞因子具有明显的平衡作用。     

Th1 and Th2 cytokine responses to academic stress.

Predominant Th2 profiles are associated with the worsening of asthma, and stress is speculated to induce a Th2 profile. The goals of this study were to examine the responses of the cytokines Th1 (IFN-gamma and IL-2) and Th2 (IL-4, IL-5, and IL-6) to a stressor and to look at the relationships between cytokine and psychological responses. Twenty-four students with and without a history of asthma completed questionnaires and gave blood samples during nonexam and exam periods. Cytokines were measured by ELISA from supernatants of stimulated mononuclear cells (MNC) and whole blood. During examinations, there were a significant decrease in IL-2 and a significant increase in IL-6 production (both cultures) and a significant decrease in IFN-gamma production (MNC cultures). Baseline IL-2 levels showed significant negative correlations with several stress and mood scores. Findings of this study indicate a down-regulation of Th1 and a selective up-regulation of Th2 cytokines during a stressful exposure.     

中等负荷运动对慢性心理应激大鼠淋巴细胞数量和Th1/Th2平衡的影响

背景:已有研究证实应激能够影响免疫功能,干扰机体的内环境稳态,而适宜运动在一定程度上能减轻不良心理应激反应.目的:观察中等负荷运动对慢性心理应激大鼠脾和肠系膜淋巴结淋巴细胞数量、血清干扰素Y和白细胞介素4水平以及Thl/Th2细胞平衡的影响.设计、时间及地点:随机对照动物实验,于2007～05/10在成都体育学院运动人体科学省级重点实验室完成.材料:40只健康雄性SD大鼠按随机数字表分为4组:对照组、心理应激组、运动组、运动+心理应激组,每组10只.方法:运动组、运动+心理应激组于实验前进行适应性游泳训练,30 min/(次·d),共3 d,实验时无负重游泳60 min,(次·d),总运动时间8周.运动+应激组在8周运动结束1 d后,开始21 d慢性束缚应激,6 h/d;心理应激组于同一时间点建立慢性束缚应激模型;运动组大鼠在8周运动结束后,同期饲养22 d;对照组大鼠同期饲养,不进行任何干预.主要观察指标:检测大鼠脾和肠系膜淋巴结淋巴细胞数量,并以ELISA法检测血清干扰素γ和白细胞介素4水平以及干扰素γ/白细胞介素4比值变化.结果:与对照组比较,心理应激组脾和肠系膜淋巴结淋巴细胞数量明显降低,血清干扰素γ及干扰素γ/白细胞介素4比值显著下降(P<0.01),Th1/Th2平衡失调.与心理应激组比较,运动+心理应激组淋巴细胞数量明显增加,干扰索γ水平和干扰素γ/白细胞介素4比值明显增高(P<0.05).与对照组比较,运动组脾和肠系膜淋巴结淋巴细胞数量虽无显著差异,但有上升趋势.运动+应激组与运动组及对照组比较,3组血清干扰素γ水平、干扰素γ,白细胞介素4比值差异无显著性意义.结论:中等负荷运动可明显增加慢性心理应激状态下脾和肠系膜淋巴结淋巴细胞数量,通过上调干扰素γ水平调节Th1/Th2的失衡,维持机体的免疫稳态.     

液相芯片法检测终末期肾病患者胞外Th1/Th2细胞因子与促炎细胞因子

目的探讨终末期肾脏病（ESRD）患者胞外Th1/Th2细胞因子的状况和临床意义。方法选取12例ESRD患者为实验组,12例健康人为对照组,运用液相芯片技术（SA）测定各实验组血清中Th1/Th2和促炎因子的水平,同时用酶联免疫吸附测定法（ELISA）定量检测细胞因子白细胞介素-8（IL-8）的水平。通过用ELISA和液相芯片法同时检测IL-8的相关性来评估液相芯片法的稳定性和准确性。结果ESRD患者血液透析前IL-10水平与对照组相比有所上升;血液透析后IL-1β水平与对照组相比降低;而血液透析前后肿瘤坏死因子-β（TNF-β）和IL-8水平均降低。ESRD患者血液透析后IL-1β、IL-4、IL-8和IL-10水平与血液透析前相比均有下降。在检测IL-8时,液相芯片法和ELISA表现出显著的相关性（P〈0.001,r=0.946）。结论血液透析后ESRD患者体内存在着细胞因子的变化,即Th2细胞因子IL-10上升而Th1细胞因子TNF-β和促炎因子IL-8降低。血液透析影响ESRD患者的细胞因子谱。     

哮喘患儿外周血Th1/Th2、Tc1/Tc2细胞检测及其意义

目的:探讨哮喘患儿急性发作期及缓解期Th1/Th2、Tc1/Tc2细胞亚群平衡的变化情况。方法:采用荧光标记抗体,应用三色荧光流式细胞仪技术,从单细胞水平对急性发作期哮喘患儿(20例)、缓解期哮喘患儿(18例)及同龄正常健康儿(对照组20例)外周血CD4+、CD8+细胞内分泌IFN-γ、IL-4进行检测。结果:哮喘患儿外周血细胞内分泌IL-4(+)的CD4+(Th2)、CD8+(Tc2)百分率较正常对照组升高,且哮喘急性发作期高于缓解期;分泌IFN-γ(+)的CD4+(Th1)、CD8+(Tc1)百分率较正常对照组降低,且哮喘急性发作期低于缓解期。结论:哮喘患儿外周血Th2、Tc2百分率升高,Th1、Tc1百分率降低,Th1/Th2、Tc1/Tc2比值降低,且哮喘急性发作期低于缓解期。     

循环毒毒蛇咬伤患者外周血Th1/Th2细胞因子的表达及意义

目的分析外周血Th1/Th2细胞因子在循环毒毒蛇咬患者中的表达,探讨其在病情评估中的意义。方法收集2016年3月至2017年10月循环毒毒蛇咬伤患者108例,根据国际蛇伤诊断标准分为轻度蛇咬伤组82例与重度蛇咬伤组26例,另外选择同期体检健康者30例为对照组。通过流式细胞术检测并比较分析各组患者T淋巴细胞亚群的分布情况及外周血Th1/Th2细胞因子的表达。结果与对照组比较,重度蛇咬伤组CD3^+、CD4^+T淋巴细胞水平偏低(P<0.01);细胞因子IL-4、IL-6、IL-10、TNF-α表达水平升高(P<0.05)。蛇咬伤组两两比较,重度蛇咬伤组CD3^+、CD4^+T淋巴细胞水平低于轻度蛇咬伤组(P<0.05),IL-4、IL-6、TNF-α表达水平均高于轻度蛇咬伤组(P<0.05)。结论循环毒毒蛇咬伤患者中存在有明显的T淋巴细胞数量的改变和Th1/Th2细胞因子表达紊乱,且与病情严重程度相关。     

特发性血小板减少性紫癜外周血Th1／Th2平衡偏移的研究

目的拟建立一种准确的、客观的检测T淋巴细胞亚群的方法，探讨在特发性血小板减少性紫癜（ITP）中T淋巴细胞亚群Th1／Th2平衡状态的变化及其意义。方法选择2007年3月至2008年2月在延安大学附属医院血液免疫科住院的30例特发性血小板减少性紫癜（ITP组）和同期20例健康体检者（对照组）作为研究对象，收集其外周抗凝血，以PE标记的抗CD195单抗和CD30单抗与FITC标记的抗CD4单抗作双色流式细胞术，用淋巴细胞设门，分别以CD4^＋ CD195^＋和CD4^＋CD30^＋细胞代表Th1、Th2细胞，分析ITP患者及健康人外周血T细胞各亚群百分率及Th1／Th2比例的变化。结果ITP组治疗前Th1、Th2细胞百分比均低于对照组，而Th1／Th2比值高于对照组，差异具有显著性（P〈0．05）；经大剂量人免疫球蛋白和地塞米松冲击治疗后，血小板数上升，Th1／Th2比值降低，与治疗前相比差异有显著性（P〈0．05）。结论在ITP患者中外周血T淋巴细胞表面标志表达异常，T细胞亚群比例失调，即ITP患者体内Th1／Th2比值升高，提示特发性血小板减少性紫癜是一种Tb1优势的疾病。