Renal Cystic Disease Induced in Newborn Rats by Diphenylamine Derivatives

The present study evaluated the effects of diphenylamine (DPA) on the kidneys of rats born of mothers fed the compound and derivatives during the last 6 days of gestation. Cystic lesions were confined to the proximal nephron in newborn rats, in contrast with the localization in the collecting ducts after long-term feeding of DPA to adult rats. Variations observed in the incidence and severity of the cystic changes in several series of animals appeared to depend upon the commercial source of the DPA. Thin-layer chromatography of DPA preparations revealed several components in addition to pure DPA. Lesions were produced in newborn offspring of pregnant rats fed microgram quantities of one of these component, whereas none were seen after feeding of chromatographically pure DPA.     

Significance of Serum Uric Acid in Pregnancy Induced Hypertension

Background and objective

Hypertension is one of the common medical complications of pregnancy and contributes significantly to maternal and perinatal morbidity and mortality. Uric acid (UA) is filtered, reabsorbed and secreted by the kidney. Thus, this study was conducted to assess the serum UA levels in PIH and to evaluate the diagnostic value of serum UA level in PIH.

升压治疗急性脑缺血再灌注损伤保护作用的实验研究

目的:探讨升压治疗局灶性脑缺血的保护作用及治疗的维持时间。方法:采用大鼠局灶性脑缺血再灌注模型,40只大鼠随机分为五组:模型对照组(A)、升压维持15min组(B)、维持45min组(C)、维持90min组(D)和120min组(E),观察各组神经功能评分、脑梗死体积和病理学改变。结果:神经功能缺损评分B、C、D组明显低于A组;B、C、D、E各组脑梗死体积明显低于A组,C、D、E组明显低于B组;各治疗组组织病理学改变比对照组轻,B组最轻。结论:缺血3h再灌注时,升压维持时间在2h以内对脑损伤有保护作用,升压最佳维持时间是15min。     

Rate and Extent of Adaptive Cardiovascular Changes in Rats during Experimental Renal Hypertension

To study the extent and exact time course of cardiovascular structural adaptation to increases in pressure load, renal hypertension was induced in normotensive male Wistar rats by renal artery constriction. At different intervals after operation the hemodynamic characteristics of the hypertensive rats and normotensive control rats were explored in paired hindquarter perfusions, from maximal dilatation up to maximal constriction (cf, Folkow et al, 1970 b). At the same time intervals the extent of left ventricular hypertrophy and of water content of the aortic wall were examined. The results reveal the presence of left ventricular hypertrophy in the hypertensive rats already after one week, soon followed by adaptive structural changes of the resistance vessels, in the form mainly of media hypertrophy, these processes being largely completed 2–3 weeks after operation. In these animals, lacking genetic predisposition for hypertension, the extent of the structural vascular changes seems large enough to explain a considerable part, but not all, of the pressure rise. An increased water content of the hypertensive aortic wall is found first 4.5 months after operation, indicating that some water logging of arterial and maybe also arteriolar walls might occur in late phases of chronic renal hypertension.     

Reduced Levels of Serum Haemolytic Complement and Renal Lesions in Ovum-Fusion-Derived Tetraparental Mouse Chimaeras

Tetraparental mice of the NZB↔CFW chimaeras were examined for: [I] the degree and location of antibody and complement deposition in the kidneys; [2] the origin of the complement deposited in the kidneys; and [3] serum haemolytic complement levels These data were compared with similar observations on pa rental-strain mice and other chimaeric combinations The renal lesions showed hath quantitatively and specially increased deposition of antibody and complement in the NZB↔CFW chimaeras compared with the controls In addition, the complement in the kidneys was shown to induce C5 of CFW origin. Finally, serum haemolytic complement in the chimaeras was undetectable despite the fact that CFW mice have rather high levels of haemolytic complement. It is argued that one explanation for these findings could be the presence of chronic allogeneic disease in the chimaeras. Other evidence for the existence of such a state it cited, but it is noted that such findings are not seen in all chimaeric combinations.     

显微分光光度计测定法对大鼠诱发性乳腺癌及其癌前病变的细胞发生研究

本实验用显微分光光度计测定DMBA诱发大鼠乳腺癌过程中的正常、增生终末蕾腺上皮和肌上皮细胞,以及癌细胞核面积与相对DNA方量。正常腺上皮细胞多为2cDNA含量,增生腺上皮和癌细胞核面积和相对DNA含量增加,与正常腺上皮之间均有极显著差异(P<0.01),直含图为分散的非整倍体模式。正常与增生肌上皮细胞的胞核面积与DNA方量无显著性差别(P>0.05),直方图为2c分布。上述实验结果提示:终末蕾细胞增生具有发展成乳腺癌的趋势,很可能为大鼠乳腺癌前病变,肌上皮细胞不参与孔腺癌的形成。     

PIH患者测定血清cTnI和Mb的临床意义

目的:探讨妊娠高血压综合征(PIH)与心肌损伤的关系.方法:采用化学发光法测定39例PIH患者血清肌钙蛋白I(cTnI)和肌红蛋白(Mb)及39例正常孕妇血清cTnI和Mb的含量.结果:PIH伴蛋白尿组血清cTnI和Mb显著高于PIH不伴蛋白尿组及正常妊娠组(P＜0.05),且随病情加重其值升高,PIH不伴蛋白尿组与正...     

低氧性肺动脉高压大鼠肺组织apelin-APJ系统的变化

目的探讨新发现的小分子活性肽apelin及其受体APJ与缺氧性肺动脉高压病理进程的关系及意义。方法清洁级雄性SD大鼠20只,随机分为正常对照组和低氧2周组。测定平均肺动脉压(mPAP)、平均颈动脉压(mCAP)及右心室与左心室+室间隔重量比(RV/LV+S),以评价缺氧性肺动脉高压模型。放免法测定血浆、肺组织匀浆apelin水平,免疫组织化学和RT-PCR分别检测肺组织apelin、APJ蛋白及基因表达的变化。结果①缺氧大鼠mPAP较对照组高124.88%(P<0.01),RV/LV+S较对照组高25.33%(P<0.01)。两组大鼠mCAP差异无统计学意义。②肺组织及血浆apelin浓度,缺氧组与对照组比较,分别为10.58±1.02vs11.17±0.73(pg/mg.pro)和479±45vs427±63(pg/ml),P均>0.05。免疫组化显示,缺氧大鼠肺组织APJ的表达较对照组低,而apelin的表达两者差异无显著性。③缺氧大鼠肺组织apelinmRNA表达水平与对照组比较差异无统计学意义(0.925±0.058vs1.021±0.036,P>0.05),而APJmRNA的表达显著低于后者(0.944±0.106vs1.199±0.053,P<0.05)。结论APJ受体表达的异常下调所致的apelin-APJ信号系统受损,在缺氧性肺动脉高压发生、发展过程中可能扮演重要角色。     

Testis Lesions and Cellular and Humoral Immune Responses Induced in Rats by Immunization With Laminin

ABSTRACT: Sixty-six percent of rats immunized with laminin isolated from a mouse Engelbreth-Holm-Swarm (EHS) sarcoma developed moderate lesions in the testis characterized by multiple foci of seminiferous tubules with different degrees of sloughing of the germinal epithelium or atrophy intermingled with normal histological areas. Interstitial mononuclear cell infiltrates were seen in the epididymis. By electron microscopy, pathological changes in the basement membranes of the seminiferous tubules, such as splitting and focal thickenings of knob-like projections toward the epithelium, were observed. Moreover, Sertoli cell cytoplasm showed dilated smooth endoplasmic reticulum and large vacuoles. By electron microscopy with the immunoper-oxidase technique, staining for in vivo-bound rat IgG was detected along the walls of the seminiferous tubules as a bright linear immunofluorescence and as a dense reaction product on the basal lamina. High titers of circulating antilaminin antibodies were detected by ELISA in all the rats immunized with laminin. As revealed by the skin test, a delayed type hypersensitivity reaction to laminin was observed in these rats.     

血清同型半胱氨酸等指标联合评价高血压患者肾损伤的临床价值

目的 探讨血清同型半胱氨酸(Hcy)、胱抑素C(Cys-C)、β2微球蛋白(β2-MG)及尿液清蛋白/肌酐比值(Alb/Cr)、尿β2-MG等指标联合评价高血压患者肾损伤的临床价值.方法 选择2013年6月到2014年6月来院诊治的226例高血压患者(根据其尿液Alb/Cr比值分为三组:正常清蛋白尿组127例,尿液Alb/Cr＜3;微量清蛋白尿组50例,尿液Alb/Cr在3 ～8.75之间;大量清蛋白尿组49例,尿液Alb/Cr＞ 8.75)和50名健康体检者,采集清晨空腹静脉血和晨尿,分别测定血清Hcy、Cys-C、β2-MG及尿液Alb、Cr、尿β2-MG,并进行统计学分析.结果 血清Hcy、Cys-C、β2-MG及尿液Alb/Cr、尿β2-MG五个指标之间均具有相关性(r:0.343～0.800,P值均＜0.01).对照组、正常清蛋白尿高血压组、微量清蛋白尿高血压组、大量清蛋白尿高血压组四组各指标总体比较,差异均有统计学意义(H:99.302～ 208.213,P值均＜0.01).四组间两两比较,正常清蛋白尿组、微量清蛋白尿组、大量清蛋白尿组血清Hcy、Cys-C、β2-MG及尿液Alb/Cr、β2-MG水平均高于对照组,差异有统计学意义(u:39.672～191.530,P值均＜0.05);正常清蛋白尿组、微量清蛋白尿组、大量清蛋白尿组血清Cys-C及尿液Alb/Cr、尿β2-MG三个指标组间差异均有统计学意义(u:37.155～191.530,P值均＜0.05),且随病情进展有增加的趋势,但血清Hcy、β2-MG组间差异无统计学意义(u:3.152～20.141,P值均＞0.05).结论 血清Hcy、Cys-C、β2-MG及尿液Alb/Cr、β2-MG五个指标均能反映高血压早期肾损伤,且血清Cys-C及尿液Alb/Cr、β2-MG三个指标能反映高血压肾损害病情发展阶段.     

血管紧张素-(1-7)对肾性高血压大鼠肾组织纤维化的影响

目的 :观察血管紧张素 ( 1 7) [Ang ( 1 7) ]对实验性肾性高血压大鼠肾组织纤维化的影响。方法 :采用微渗泵植入技术 ,建立Ang ( 1 7)对二肾一夹 ( 2K1C)高血压大鼠干预模型 ,在光镜下观察肾脏纤维化 ,免疫组化法检测肾组织转化生长因子β1 (TGF β1 ) ,RT PCR检测肾组织内TGF β1 、TGF β1 受体ImRNA水平。结果 :Ang ( 1 7)能减轻2K1C高血压大鼠肾纤维化 ,减少 2K1C高血压大鼠肾组织TGF β1 的蛋白表达 ,降低肾组织内TGF β1 、TGF β1 受体ImRNA水平。结论 :Ang ( 1 7)对 2K1C高血压大鼠肾纤维化作用机制之一是通过减少肾组织内TGF β1 及其受体的表达而实现