聚酯型儿茶素对小鼠的免疫调节作用

目的 :研究聚酯型儿茶素对正常小鼠及实验性免疫功能低下小鼠的影响。方法 :选用二硝基氟苯 (DNFB)致小鼠迟发型超敏反应 (DTH)模型、小鼠腹腔巨噬细胞 (PMΦ )吞噬鸡红细胞模型以及血清溶血素生成法 (HC50) ,分别观察对正常小鼠特异性细胞免疫、非特异性细胞免疫和体液免疫功能的影响。以环磷酰胺腹腔注射15mg/(kg·d)×4d造成免疫功能低下的模型 ,采用DTH、碳粒廓清法及血清溶血素生成法测定有关免疫指标。结果 :聚酯型儿茶素 (50、100、200mg/(kg·d)×7d)可明显增强正常小鼠的耳片肿胀度、腹腔巨噬细胞吞噬功能及血清溶血素生成 ,明显提高环磷酰胺所致免疫低下小鼠的DTH反应、碳粒廓清K和α值及HC50 值。结论 :聚酯型儿茶素对正常及免疫功能低下小鼠的细胞免疫和体液免疫均具有增强作用。     

柴胡乳剂对小鼠免疫功能的影响

目的研究中药柴胡乳剂对小鼠免疫功能的影响。方法分别用碳粒廓清法、溶血素测定法和2,4二硝基氯苯诱导DTH反应法测定柴胡乳剂对正常小鼠非特异性免疫及环磷酰胺免疫功能低下小鼠体液免疫、细胞免疫的影响。结果与正常对照组比,柴胡乳剂组小鼠的碳粒廓清指数明显提高;柴胡乳剂能明显提高环磷酰胺免疫功能低下小鼠血清溶血素含量,增加DTH反应小鼠的耳肿胀度。结论柴胡乳剂可以增强正常小鼠及Cy所致免疫功能低下小鼠的免疫功能。     

白芍总甙对免疫应答的调节作用

白芍总甙(TGP)200mg/kg/d ig×8d对小鼠迟发型超敏反应(DH)有增强作用。TGP5mg/kg/d ip×8d(或5d)对正常小鼠DH的影响并不恒定,或呈增强或呈抑制或无明显作用;对环磷酰胺诱导的DH增强和抑制都有明显对抗作用,但对地塞米松诱导的小鼠DH抑制无明显的影响。TGP5mg/kg/d ip×4d不影响正常小鼠抗体的生成量,但能使环磷酰胺诱导免疫低下小鼠的抗体生成量恢复到正常对照水平。TGP40mg/kg/d ig×3d能促进小鼠腹腔巨噬细胞的吞噬功能。上述结果表明TGP对小鼠免疫应答具有调节作用。     

油菜蜂花粉对小鼠免疫功能的影响

以10g/kg油菜蜂花粉给NIH小鼠灌胃,对环磷酰胺所致免疫功能低下及白细胞减少有明显的对抗作用,而且还能提高小鼠腹腔巨噬细胞的吞噬功能和血液中碳粒廓清速率。     

国产环孢素A对小鼠免疫功能的抑制作用

当剂量为25～100mg/kg·d~(-1),ig×4d时,国产环孢素A(CsA)显著抑制小鼠脾脏空斑形成细胞数和溶血素生成,并呈剂量依赖方式。该剂量给药10d,可显著抑制2,4—二硝基氯苯所致小鼠迟发型皮肤超敏反应。CsA(50 mg/kg·d~(-1),ig×14d)能明显延长小鼠移植心脏的存活时间。CsA(50,100mg/kg·d~(-1),ig×4d)对小鼠iv碳粒廓清速率和骨髓细胞数均无明显影响。国产CsA和进口CsA对小鼠脾脏空斑形成细胞反应的抑制作用无显著差异。     

MS-870对小鼠免疫功能的影响

MS—870(50mg·Kg~(-1)·d~(-1)×7d.po)能显著促进正常小鼠和环磷酰胺所致的免疫反应低下小鼠溶血素生成,提高外周血酸性非特异性酯酶染色T淋巴细胞阳性百分率和增强小鼠迟发型变态反应.体外(10μg、5μg·L~(-1))明显促进NK细胞活性。此外.MS—870尚能提高碳粒廓清速率巨噬细胞吞噬率与吞噬指数.并使免疫器官增重。     

蜜环菌多糖对小鼠免疫功能的影响

蜜环菌多糖(100mg/kg·d×5d,ig)能显著增加正常小鼠和注射环磷酰胺所致免疫功能抑制小鼠的溶血素生成。当剂量为50mg/kg·d×5d,ig时,它还能显著增加正常小鼠的空斑形成细胞数。体外试验,蜜环菌多糖(10、50μg/ml)显著增强刀豆素A诱导的小鼠淋巴细胞增殖反应,但对二硝基氯苯所致小鼠迟发型过敏反应无显著增强作用。此外,蜜环菌多糖还能增加小鼠静注碳粒廓清速率及腹腔巨噬细胞的吞噬百分数和吞噬指数。     

党参多糖对小鼠细胞免疫调节作用

给小鼠ip CPP 200mg/kg·d~(-1)×5d或8d对腹腔Mφ吞噬CRBC、小鼠碳粒廓清和胸腺细胞E花环形成有促进作用,并对ip CY、HCT鼠腹腔Mφ吞噬功能、E花环形成有增强和恢复作用。对DNCB诱发的正常鼠DTH有抑制作用,而对DMS鼠DTH有恢复作用。     

白芍总甙调节小鼠免疫功能的机理

用单克隆抗体间接免疫荧光法观察白芍总甙(TGP)调节小鼠免疫功能的部分机理。TGP(5 mg/kg·d~(-1)×8d,ip)拮抗环磷酰胺(Cy)所致小鼠DTH反应增高,与其使L3T4~+/Lyt-2~+细胞比值降低有关;TGP(5mg/kg·d~(-1)×5d,ip)拮抗Cy所致小鼠DTH反应低下及脾细胞抗SRBC溶血素的减少,与其使该比值增高有关。提示TGP对小鼠免疫功能的调节作用与其调节Th/Ts细胞比值有关。     

肝舒胶囊对小鼠的免疫调节作用

目的 探讨肝舒胶囊的免疫调节作用.方法测定环磷酰胺(CY)免疫抑制小鼠的血清溶血素和正常小鼠的血清溶血素;测定氢化可的松(HC)免疫抑制小鼠网状内皮系统吞噬功能碳粒的廓清指数;测定二硝基氯苯(DNCB)诱导小鼠迟发型超敏反应小鼠耳的肿胀度.结果1.9、0.95 g·kg-1肝舒胶囊对CY所致小鼠血清溶血素低下有显著提高...     

淫羊藿总黄酮的免疫调节作用

淫羊藿总黄酮４００ｍｇ／ｋｇ显著增加正常小鼠单核巨噬细胞的吞噬功能,提高血清溶血素抗体生成水平,对迟发型超敏反应强度无明显影响;２００ｍｇ／ｋｇ,４００ｍｇ／ｋｇ可显著拮抗环磷酰胺所致小鼠单核巨噬细胞吞噬能力,血清溶血素抗体生成水平和迟发型超敏反应强度降低;２００ｍｇ／ｋｇ可显著降低致敏前给予环磷酰胺所致的迟发型超敏反应增强．进一步研究表明;淫羊藿总黄酮的免疫调节作用与其对ＴＨ／ＴＳ比值的调节作用有关．     

褪黑素对光暗周期频繁颠错小鼠免疫功能的调节作用

目的研究光暗周期颠倒 (light darkshifting,LDS)对小鼠免疫功能的影响 ,以及褪黑素 (mela tonin,MT)对LDS小鼠的免疫调节作用。方法在建立LDS的动物模型上测定抗体形成细胞(PFC)溶血实验 ,血清溶血素水平 ,迟发超敏反应 ,白细胞数及淋巴细胞百分率及碳粒廓清指数。结果LDS使抗体形成细胞的溶血能力升高 ,血清溶血素水平上调 ,迟发免疫功能亢进 ,白细胞数和淋巴细胞百分率下降 ;MT使LDS小鼠降低的白细胞数及淋巴细胞百分率明显升高 ,下调血清溶血素及PFC水平、迟发超敏反应 ,使之趋于正常 ,对LDS小鼠碳粒廓清能力无显著性影响。结论MT使LDS所致免疫功能异常调节到正常水平。     

10-羟基-2-癸烯酸对小鼠T淋巴细胞及其亚型和白介素2产生的影响

观察了10-羟基-2-癸烯酸（10-HDA）对小鼠#FST#FK淋巴细胞及其亚型和白介素2产生的影响. 结果表明，10-HDA 1, 5, 25 mg·kg^-1·d^-1 ip, 5 d可拮抗环磷酰胺100 mg·kg^-1对小鼠迟发型超敏反应（DTH）的抑制作用. 体外给药，10-HDA可促进刀豆球蛋白A诱导的T淋巴细胞增殖反应；促进小鼠脾细胞产生白介素2. 采用单克隆抗体间接免疫荧?光法证明10-HDA可增加小鼠胸腺L₃T₄⁺细胞数，而对Lyt-2⁺细胞无明显影响. 结果提示10-HDA可调节T淋巴细胞参与的免疫反应.     

Cholinergic and opiate involvement in the antinociceptive effect of diisopropylfluorophosphate

The organophosphate diisopropylfluorophosphate (DFP; 3 or 6 mg/kg, IP) caused a dose-related antinociception in mice which was antagonized by the muscarinic antagonist scopolamine. The opiate antagonist naloxone antagonized the antinociceptive effect of the highest dose of DFP, but did not affect the antinociception caused by 3 mg/kg DFP. Twenty-four hours after the administration of DFP, reaction time in animals which received a 3 mg/kg dose did not differ from control. However, reaction time was still significantly higher than control in mice administered 6 mg/kg DFP twenty-four hours earlier. This residual antinociception was antagonized by naloxone but not by scopolamine, suggesting that it was opioid in nature. These results suggest that antinociception induced by a low dose of DFP is primarily due to a cholinergic mechanism, while higher doses appear to affect also the opiate system. Since we have previously shown that DFP (6 mg/kg) increases met-enkephalin levels in brain, it is possible that high doses of DFP might interfere with enkephalin metabolizing enzymes. This conclusion cannot be extended to the organophosphate disulfoton, whose antinociception, even at high doses, appears to involve only an interaction with the cholinergic system.     

Neurobiological responses to ethanol in mutant mice lacking neuropeptide Y or the Y5 receptor

We have previously shown that voluntary ethanol consumption and resistance are inversely related to neuropeptide Y (NPY) levels in NPY-knockout (NPY -/-) and NPY-overexpressing mice. Here we report that NPY -/- mice on a mixed C57BL/6Jx129/SvEv background showed increased sensitivity to locomotor activation caused by intraperitoneal (ip) injection of 1.5 g/kg of ethanol, and were resistant to sedation caused by a 3.5-g/kg dose of ethanol. In contrast, NPY -/- mice on an inbred 129/SvEv background consumed the same amount of ethanol as wild-type (WT) controls at 3%, 6%, and 10% ethanol, but consumed significantly more of a 20% solution. They exhibited normal locomotor activation following a 1.5-g/kg injection of ethanol, and displayed normal sedation in response to 2.5 and 3.0 g/kg of ethanol, suggesting a genetic background effect. Y5 receptor knockout (Y5 -/-) mice on an inbred 129/SvEv background showed normal ethanol-induced locomotor activity and normal voluntary ethanol consumption, but displayed increased sleep time caused by 2.5 and 3.0 g/kg injection of ethanol. These data extend previous results by showing that NPY -/- mice of a mixed C57BL/6Jx129/SvEv background have increased sensitivity to the locomotor activation effect caused by a low dose of ethanol, and that expression of ethanol-related phenotypes are dependent on the genetic background of NPY -/- mice.