烧伤病人TNF,IL—6的变化与氧自由基关系的研究

研究烧伤后ＴＮＦ、ＩＬ－６的变化与氧自由基的关系。采用酶标法检测血清ＴＮＦ和ＩＬ－６，改良八木国夫法检测血清ＭＤＡ，动态观察了烧伤病人伤后１０ｄ内血清ＴＮＦ、ＩＬ－６和ＭＤＡ的变化以及抗氧化剂对血清ＴＮＦ和ＩＬ－６的影响。结果显示烧伤病人血清ＴＮＦ、ＩＬ－６和ＭＤＡＦ均显著增高（Ｐ〈０．０１）；ＴＮＦ与ＭＤＡ的变化呈正相关（ｒ＝０．４５，Ｐ〈０．０５）；抗氧化剂可降低血清ＴＮＦ（Ｐ〈０．０５）和Ｉ     

细胞因子在免疫复合物型肾小球肾炎发病机制中的作用

按Ｄｉｘｏｎ方法制造血清病型肾小球肾炎动物模型，进而研究其发病机制，模型ＡＥＳＳＲ血清ＣＩＣ水平明显高于正常值（Ｐ〈０．０１）；ＣＭＳＣ水平明显低于正常值（Ｐ〈０．０１），ｓＩｌ－２Ｒ，ＩＬ－８，ＩＦＮ，ＴＮＦ和ＩＬ－２水平均明显高于正常值（Ｐ〈０．０１），ＣＩＣ与ＣＭＳＣ呈高度负相关，ｒ＝－０．９４３（Ｐ〈０．０５），ＣＩＣ与ＩＬ－８呈高度正相关，相关系数ｒ＝０．８２９（Ｐ〈０．０５）。进一步证     

TNF检测在环磷酰胺治疗狼疮性肾炎中的价值

目的 探讨肿瘤坏死因子（ＴＮＦ）检测在大剂量环磷酰胺静脉冲击治疗狼疮性肾炎（ＬＮ）中的价值。方法 用ＥＬＩＳＡ双抗体夹心法检测５１例ＬＮ患者环磷酰胺静脉冲击治疗（ＩＶ－ＣＴＸ）前后血清和尿ＴＮＦ水平。结果 活动期ＬＮ患者血清和尿ＴＮＦ水平显著高于稳定性（Ｐ〈０．００１）。血甭ＴＮＦ水平与血沉呈显著正相关（ｎ＝５１,ｒ＝０．３８６,Ｐ〈０．０５）。轻度肾功不全患者血清ＴＮＦ显著高于肾功正常（Ｐ,０．     

葡萄胎患者化疗前后SIL—2R和TNF含量检测的临床意义

分析了３２例葡萄胎患者化职前后血清中ＳＩＬ－２Ｒ和ＴＮＦ含量的变化。结果表明：化疗前血清中ＳＩＬ－２Ｒ和ＴＮＦ含量均显著地高于正常对照组（Ｐ〈０．０１，Ｐ〈０．００１），化疗后均有明显的下降（Ｐ〈０．０５），ＳＩＬ－２Ｒ和ＴＮＦ与β－ＨＣＧ呈正相关（ｒ＝０．６２６３，ｒ＝０．６１０５，Ｐ〈０．０１）。机理不明。结果提示：血清ＳＩＬ－２Ｒ和ＴＮＦ可作为葡萄胎患者免疫功能监测指标之一，并可作为疗效判断     

原发性肝癌患者血清可溶性细胞间粘附分子-1检测

应用酶联免疫吸附法检测了３８例原发性肝癌（ＰＨＣ）患者血清ｓＩＣＡＭ－１含量,结果表明：⑴ＰＨＣ患者血清ｓＩ ＣＡＭ－１水平明显高于健康对照,肿瘤直径≥５ｃｍ或结节≥３个组血清ｓＩ ＣＡＭ－１水平高于肿瘤直径〈５ｃｍ或结节〈３个组,静脉癌栓形成组（Ｐ均小于０．００１）;⑵ＰＨＣ患者血清ｓＩ ＣＡＭ－１水平与血清Ｂｉｌ－Ｔ、ＡＳＴ含量呈明显正相关（Ｐ〈０．０５）,而与血清ＡＦＰ含量无明显正相关（Ｐ〉     

原发性肝癌患者手术治疗前后检测可溶性白细胞介素2受体和肿瘤坏？…

应用双抗体酶联夹心法和放射免疫分析法检测了３０名正常人和３３例原发性肝癌患者血清中ｓＩＬ－２Ｒ和ＴＮＦ含量，结果表明：原发性肝癌患者手术前ｓＩＬ－２Ｒ，ＴＮＦ非常显著地高于正常人（Ｐ〈０．００１，Ｐ〈０．０１）；术后２周，血清ｓＩｌ－２Ｒ与正常人比较有差异（Ｐ〈０．０１），而ＴＮＦ则无差异（Ｐ〉０．０５），ＡＦＰ含量高低与ｓＩｌ－２和ＴＮＦ含量无关（ｒ＝０．３８２６，０．３７２５；Ｐ〉０．０５）。     

冠状动脉造影后血浆肾上腺髓质素的改变

本文研究冠状动脉造影前后血浆肾上腺髓质素（ＡＤＭ １３－ ５２）及内皮素（ＥＴ－ １）水平。用特异性放射免疫法测定冠状动脉正常人和冠状动脉病患者，冠脉造影前后血浆ＡＤＭ（１３－５２）及ＥＴ－１含量。结果表明冠脉造影后即刻血浆ＡＤＭ（１３－５２）和ＥＴ－１均明显升高，ＡＤＭ（１３－５２）从基础时３１．２３ ±１．１２ｐｇ／ｍＬ升高到４０．９１±１．２８ｐｇ／ｍＬ（Ｐ＜０．００１），ＥＴ－１从８８．８４±１０．５８ｐｇ／ｍＬ升高到１５８．２６ ± １３．６６ｐｇ／ｍＬ（Ｐ＜０．０１）。正常组及冠脉病组冠造术后血浆ＡＤＭ（１３－５２）及ＥＴ－１均较术前明显升高；但两组间比较，无论冠脉造影前后，ＡＤＭ或ＥＴ－１均无显著差异。ＡＤＭ（１３－５２）与ＥＴ－１的变化呈中等度的正相关（ｒ＝０．４４８４， Ｐ＜ ０． ００１）。因此，冠脉造影可使ＡＤＭ（１３－５２）及ＥＴ－１均升高，可能与造影剂对血管刺激、导管直接对血管内皮的刺激及交感神经兴奋性增高有关。ＥＴ—１可能作为一种中介因子，与冠脉造影的部分并发症有关，而与之作用相反的ＡＤＭ（１３－５２）升高可能是机体维持自身稳定的一种代偿性保护机制。     

再生障碍性贫血患者免疫调节剂治疗前后TNF和IL—2水平变化及意义

为了探讨再生障碍性贫血（ＡＡ）的免疫发病机理及抗Ｔ淋巴细胞单克隆抗体（ＭｃＡｂ－Ｔ）的免疫调节治疗作用，采用放射免疫法检测３０例ＡＡ患者ＭｃＡｂ－Ｔ治疗前后血清肿瘤坏死因子（ＴＮＦ）和白细胞介素－２（ＩＬ－２）水平及其中１０例ＡＡ外周血核细胞（ＰＢＭＮＣ）体外诱生ＴＮＦ和ＩＬ－２水平的变化。结果表明，治疗前ＡＡ患者血清ＴＮＦ水平显著曾高（Ｐ〈０．０１），ＰＢＭＮＣ诱生的ＴＮＦ和ＩＬ－２水平均明显高     

地奥心血康对心肌缺血再灌犬血清肾上腺素含量，心脏指数及总外周血管阻力的影响

杂种犬随机分为地奥心血康治疗组（ＤＫＧ）及生理盐水对照组（ＣＧ）。应用荧光法测定血清去甲肾上腺素（ＮＥ）含量，定时测定平均动脉压（ＭＢＰ），心输出量（ＣＯ），计算总外周血管阻力（ＴＰＶＲ），心脏指数（ＣＩ）。结果显示：（１）再灌后的ＭＢＰ在ＤＫＧ与ＣＧ之间无明显差异，Ｐ＞０．０５；（２）再灌２４０ｍｉｎ时，ＤＫＧ中ＣＩ值高于ＣＧ（Ｐ＜０．０１）；ＮＥ值与ＴＰＶＲ值明显低于ＣＧ（Ｐ＜０．０５，Ｐ＜０．０１）；（３）ＤＫＧ中ＮＥ与ＣＩ无相关性，ＣＧ中二者呈负相关，ｒ＝－０．６８３１，Ｐ＜０．０１；（４）两组ＣＩ与ＴＰＶＲ均呈负相关，ｒ＿（ＣＧ）＝－０．７７８３，Ｐ＜０．００１，ｒ＿（ＤＫＧ）＝－０．５２１８，Ｐ＜０．００１。     

病毒性肝炎患者血清sICAM-1、IL-6及TNF-α水平检测分析

应用酶联免疫吸附法检测了４３名对照组和１２９例各类病毒性肝炎患者血清ｓＩＣＡＭ－１、ＩＬ－６及ＴＮＦ－α水平含量。结果表明：急、慢性肝炎和肝硬化患者血清ｓＩＣＡＭ－１水平明显高于对照组（Ｐ〈０．０１）,慢性重型肝炎组明显高于中度和轻度肝炎组（Ｐ〈０．０１）,各类肝炎患者血清ｓＩＣＡＭ－１水平与ＡＬＴ、ＴＢｉｌ均呈明显正相关;各类肝炎及肝硬化患者血清ＩＬ－６和ＴＮＦ－α水平也较对照组显著升高（Ｐ〈０     

急性心肌梗塞病人细胞粘附分子与补体激活成分的动态变化

目的:探讨急性心肌梗塞(AMI)病人血浆细胞粘附分子和补体活化成分的变化。方法:采用酶联免疫吸附法(ELISA),检测了67例AMI病人发病第1、4、7d时和38例健康人,42例陈旧性心肌梗塞(OMI)病人白细胞CD18表达、血浆可溶性细胞间粘附分子-1(sICAM-1)、可溶性血管细胞间粘附分子-1(sVCAM-1)和血浆补体活化片段(sC5b-9)浓度的变化。结果:AMI病人白细胞CD18表达、sICAM-1、sVCAM-1和sC5b-9浓度非常显著高于对照组和OMI病人(P＜0.01)。发病第1-7d,白细胞CD18表达、sICAM-1、sVCAM-1和sC5b-9浓度逐渐降低。死亡者和伴有室性心律失常者各指标增高较存活者和无室性心律失常者更明显(P＜0.01)。AMI病人白细胞CD18表达、sICAM-1、sVCAM-1浓度与sC5b-9浓度呈正相关(r=0.648,0.652,0.668,0.698,0.914,0.725,0.737,0.752,0.792,P＜0.01),白细胞CD18表达与sICAM-1、sVCAM-1浓度呈正相关(r=0.662,0.683,0.695,0.738,0.744,0.745,P＜0.01)。结论:细胞粘附分子和补体激活成分的相互作用参与了AMI的发生和发展,且与病情严重程度和预后有密切关系。     

围手术期血浆ET-1/CGRP比值的变化

本文研究围手术期血浆内皮素－１和降钙素基因相关肽水平的动态变化及其意义。选择胃癌和结直肠癌患者,分别取术前、术中和术后第１、７天外周静脉血。     

The interaction of tumour necrosis factor alpha and endothelin-1 in pathogenetic models of asthma

Background There is evidence that tumour necrosis factor alpha (TNFα) may be an important mediator in initiating asthmatic airway inflammation. It has been proposed that endothelin-1 (ET-1) is involved in bronchoconstriction and airway remodelling in asthma. It is not known, however, if there is any interaction between TNFα and ET in perpetuating airway inflammation in asthma. Objective The present study aimed to determine the activities of ET-1 and TNFα in ovalbumin-sensitized guinea pigs and their roles in the development of airway inflammation. Method Twelve guinea pigs were sensitized by ovalbumin injection and aerosol inhalation. ET-1 levels were measured in both bronchoalveolar lavage fluid (BALF) and plasma by ¹²⁵-labelled endothelin-1 (ET-1) radioimmunoassay. The TNFα activity released from alveolar macrophage (AM) in BALF was estimated by ELISA. Cultured bovine airway smooth muscle cells (BASMCs) were treated with TNFα (1000 units/5 ± 10⁴ cells) for different times. ET-1 levels in harvested medium from these cells were measured by radioimmunoassay. Cultured human fetal lung fibroblasts (HFLFs) were incubated with ET-1(10^–8–10^–6M), then ³HTdR incorporation to these cells and cell counting were performed. The effects of ET-1 stimulation on the granulocyte macrophage colony stimulating factor (GM CSF) gene expression in HFLFs were estimated by using RT- PCR method. Results ET-1 levels in both plasma and BALF were significantly higher in ovalbumin- sensitized guinea-pigs compared with those in controls (422.27 × 175.0pg/mL vs 277.311 × 88.0pg/mL, P < 0.05, 81.22 × 16.15 vs 49.81 × 12.64pg/mL, P < 0.05) while TNFα activity was also significantly increased in the OVA-sensitized group compared with that in the control group (6010 ± 1900pg/mL vs 2810 × 450 pg/mL, P < 0.05). The ET-1 level in harvested medium of BASMCs rose significantly in 12 h in the TNF-α treated group (from < 5pg/mL to53.72 × 14.3pg/mL, P < 0.001), and remained at a similar level for 24 h in the TNFα treated group. It was shown that ET-1 not only stimulated cell proliferation but also induced GM-CSF mRNA expression in HFLFs. Conclusion ET-1 levels in both plasma and BALF and TNFα release from macrophage are increased significantly in ovalbumin-sensitized guinea-pigs. TNFα stimulates ET-1 secretion from cultured BASMCsw; ET-1 accelerates cell proliferation and induces GM- CSF mRNA expression in the human fetal lung fibroblast.     

Homocysteine,endothelin-1 and nitric oxide in patients with hypertensive disorders complicating pregnancy

Objective: To investigate the change of level of serum homocysteine (Hcy), endothelin-1 (ET-1) and nitric oxide (NO) and clinical significance in patients with HDCP. Methods: Two hundred and thirty nine patients with HDCP (137 patients with mild preeclampsia, 102 patients with severe preeclampsia) who were hospitalized between June 2012 and June 2015 and 200 normal pregnancy women in outpatient department were enrolled in our study were divided into HDCP group and control group. Serum Hcy concentration was measured by enzymatic cycling assay. ET-1 concentration was measured by enzyme linked immunosorbent assay. And no concentration was measured by nitrate reductase assay. Results: Serum Hcy and ET-1 in HDCP group were significantly higher as compared to control group (P<0.05). Level of serum NO in HDCP group was significantly lower than in the control group (P<0.05). Level of serum Hcy and ET-1 in mild and severe preeclampsia group were significantly higher as compared to control group, respectively (P<0.05). Level of serum NO in mild and severe preeclampsia group were significantly lower than in the control group’ respectively (P<0.05). Level of serum Hcy and ET-1 in severe preeclampsia group were significantly higher as compared to mild preclampsia group (P<0.05). Level of serum NO in severe preeclampsia group were significantly lower than in mild preeclampsia group (P<0.05). Spearman rank correlation analysis showed that level of serum Hcy and ET-1 was positively correlated with severity of diseases (r=0.689, 0.718, P<0.05). Level of serum NO was negatively correlated with severity of diseases (r=-0.702, P<0.05). Conclusion: Serum Hcy, ET-1 and NO were associated with pathogenesis of HDCP. Comprehensively measurement of them could effectively evaluate the incidence and progress of HDCP.     

急性冠脉综合征患者血浆B型钠尿肽水平及影响因素分析

目的:分析急性冠脉综合征(ACS)患者血浆B型钠尿肽水平及影响因素和临床意义。方法:平行测定82例ACS,包括50例急性心肌梗死(AMI)、32例不稳定型心绞痛(UAP)患者、38例稳定型心绞痛(SAP)患者和30例非冠心病对照者的血浆BNP、血清肌钙蛋白I(cTnI)、左室射血分数(LVEF)、超敏C反应蛋白(hs-CRP)和白细胞(WBC)计数;分析ACS与另两组相关指标变化的差异及对BNP水平的影响。结果:AMI组和UAP组BNP水平分别为683.84士561.27pg/ml、386.05士201.54pg/ml,均明显高于对照组(55.67士35.46pg/ml),P均<0.01;AMI组BNP水平显著高于SAP组(201.21士105.0pg/ml,P<0.05)。直线相关分析显示ACS患者BNP水平升高与cT-nI、WBC、hs-CRP呈显著正相关(r分别为0.615、0.286、0.506,P均<0.05),与LVEF呈显著负相关(r=-0.714,P<0.01)。多元线性逐步回归分析发现cTnI、hs-CRP、LVEF与BNP独立相关(标准化偏回归系数β分别为0.391、0.240和-0.426,P<0.05)。结论:ACS患者血浆BNP水平升高可用于评价心肌缺血、坏死、炎症反应和心功能损伤程度。     

体外反搏对血液不对称二甲基精氨酸和内皮素的影响

目的：经皮冠状动脉腔内成形和支架植入术（PTCA+stent）后的冠心病患者经过增强型体外反搏治疗后，观察其血清不对称二甲基精氨酸（ADMA）和内皮素-1（ET-1）水平的变化，从而反映体外反搏对血管内皮功能的影响。方法:将51名冠心病患者（PTCA+stent后）分成两组，按 1∶2 匹配，反搏组17人，对照组34人。两组均给予常规的药物治疗，此外，反搏组予以3个疗程的体外反搏治疗。ADMA采用高效液相色谱-荧光法检测，ET-1采用放射免疫法检测。结果:反搏组在治疗后ADMA和 ET-1水平明显低于治疗前（P<0.05）；对照组治疗前后ADMA差异无显著（P>0.05），ET-1在治疗后高于治疗前（P<0.01）。组间比较，反搏组的ADMA和ET-1降低程度明显多于对照组（P<0.01）；反搏组心绞痛症状改善和心绞痛减少次数均较对照组明显（P<0.01），并且ADMA降低的程度与两者呈正相关（r分别是0.85、0.70，P<0.01） 。结论：增强型体外反搏通过提高对血管内皮细胞的切应力，降低了PTCA+stent术后冠心病患者血液中的ADMA、ET-1，提示血管内皮功能的改善，为冠心病患者的综合治疗提供了实验依据。     

Endothelin-1 in patients with complicated and uncomplicated myocardial infarction

Summary Endothelin-1 concentrations were measured in peripheral venous blood samples from 42 patients with acute myocardial infarction. In patients with ischemic or hemodynamic complications (n = l1), endothelin-1 concentrations were significantly higher already on admission (P = 0.008) and remained significantly higher until day 6 after admission compared to patients with uncomplicated infarctions (n = 31; P = 0.035). There were no close correlations between peak concentrations of endothelin-1 and creatine kinase or creatine kinase isoenzyme MB mass in either group. Only in complicated patients did left ventricular ejection fraction correlate closely and inversely with peak endothelin-1 concentrations (r = –0.71; P = 0.03). Therefore, plasma endothelin-1 concentrations in patients with acute myocardial infarction patients may reflect states of markedly depressed cardiac performance and recurrent myocardial ischemia.Abbreviations AMI acute myocardial infarction - CK creatine kinase - CKMB mass CK isoenzyme MB mass - LVEF left ventricular ejection fraction - ET-1 endothelin-1     

妊高征肾病患者血浆ET-1和血清TGF-β1、IL-10检测及其临床意义

目的：探讨妊高征肾病患者治疗前后血浆ET-1和血清TGF-β1、IL-10水平的变化及临床意义。方法：应用放射免疫分析和酶联法对32例妊高征肾病患者进行了治疗前后血浆ET-1和血清TGF-β1、IL-10检测,并与35名正常人作比较。结果：妊高征肾病患者在治疗前血浆ET-1和血清TGF-β1、IL-10均非常显著地高于正常人组（P〈0.01）,经治疗2周后则与正常人组比较无显著性差异（P〉0.05）,血浆ET-1和血清TGF-β1、IL-10水平呈正相关（r=0.4812、0.5784,P〈0.01）结论：检测妊高征肾病患者血浆ET-1和血清TGF-β1、IL-10水平的变化,对患者的病情判断、疗效观察具有重要的临床价值。     

大骨节病患者血清促炎症细胞因子水平的检测

目的探讨前炎症细胞因子TNF、IL-1β和IL-6在大骨节病(KBD)发病机制中的作用。方法采集62例KBD患者和60例健康对照的血清标本,采用双抗体夹心ELISA法测定血清前炎症细胞因子TNF、IL-1β和IL-6的水平。结果KBD患者血清IL-1β和IL-6的水平分别为(238.4±698.5)ng/L和(164.4±661.4)ng/L,健康人分别为(74.5±130.0)ng/L和(52.2±154.6)ng/L,但它们之间差异均不显著(P>0.05)。然而,KBD患者血清TNF的水平[(109.2±145.3)ng/L]高于健康对照[(40.9±89.7)ng/L],差异非常显著(P<0.01)。患者血清TNF与IL-1β的水平及血清TNF与IL-6水平的相关性均不显著(r值分别为0.0387和0.2135,P>0.05)。血清IL-1β与IL-6的水平呈显著的正相关(r=0.3460,P<0.01)。结论血清前炎症细胞因子水平的升高,可能与大骨节病的发病有关。