新生儿感染性疾病红细胞免疫功能及SOD的变化

对５种临床常见的新生儿感染性疾病（败血症、肺炎、脐炎、臀红、鹅口疮）的红细胞Ｃ３ｂ受体花环率（ＲＢＣ－Ｃ３ｂＲＲ）、免疫复合物花环率（ＲＢＣ－ＩＣＲ）、血清中红细胞免疫粘附促进因子（ＲＦＥＲ）及抑制因子（ＲＦＩＲ）以及红细胞超氧化物歧化酶（ＳＯＤ）的活性进行了检测，结果表明红细胞免疫功能及ＳＯＤ的变化与感染的严重程度及疾病的不同阶段密切相关。     

正常新生儿红细胞免疫功能观察

本文对43例正常新生儿红细胞免疫功能进行了测定,新生儿红细胞C_3b受体(C_3bR)花环率为22.04±6.56％,红细胞免疫复合物(IC)花环率为14.82±8.12％,均明显高于正常产妇、成人及儿童,P<0.01差异有十分显著意义,表明新生儿红细胞免疫功能增强,提示可能是新生儿抗母体排斥的一种正常反应。     

贫血患儿红细胞免疫功能观察

为了解贫血患儿的红细胞免疫功能,对 ８５例贫血患儿（缺铁性贫血 ２９例、急性白血病 ２６例、地中海贫血 １８例、再生障碍性贫血 １２例）进行了红细胞 Ｃ３ｂ受体花环率（ＲＢＣ－Ｃ３ｂＲＲ）和红细胞免疫复合物花环率（ＲＢＣ－ＩＣＲ）测定。结果显示所有贫血患儿ＲＢＣ－Ｃ３ｂＲＲ均显著低于对照组儿童（Ｐ＜０．０１）,缺铁性贫血和再生障碍性贫血患儿 ＲＢＣ－ＩＣＲ与对照组比较无明显差异（Ｐ＞０．０５）,急性白血病患儿 ＲＢＣ－ＩＣＲ高于对照组（Ｐ＜０．０５）,地中海贫血患儿 ＲＢＣ－ＩＣＲ则显著高于对照组（Ｐ＜０．０１）。贫血患儿容易感染可能与红细胞免疫功能低下有关。     

新生儿缺氧缺血性脑病红细胞免疫功能的变化及其影响因素

目的了解新生儿缺氧缺血性脑病(hypoxic-ischemicencephalopathy,HIE)的红细胞免疫功能状态及各种因素对其的影响。方法采用郭峰红细胞酵母花环法对73例HIE检测红细胞C3b受体花环率(RBC-C3bRR)、红细胞免疫复合物花环率(RBC-ICR),并与104例正常新生儿进行对照。结果①HIE患儿的RBC-C3bRR低于正常对照组,其RBC-ICR高于对照组,差异有显著性(P<0.01);②中、重度HIE患儿的RBC-C3bRR低于轻度HIE患儿(P<0.01);不同程度的HIE患儿间RBC-ICR差异无显著性;③HIE伴出血、硬肿症患儿的RBC-ICR增高,而RBC-C3bRR差异无显著性。④HIE患儿的红细胞免疫功能与性别、胎龄、日龄、脐带、羊水、Apgar评分等因素无明显相关性。结论新生儿HIE的红细胞免疫功能低下,而且HIE病情程度越严重,红细胞免疫功能低下越明显。     

新生儿血清胆红素对血钙浓度的影响

本文对１０４例黄疸新生儿的血钙进行了测定。结果显示：１．高胆组的血清游离钙浓度显著高于非高且，而结合钙的差异无显著性；２．血清非结合胆红素值与血清游离钙水平呈正相关。可见，胆红素影响了细胞钙的分布，这可能与胆红素的神经毒有关。     

围生期新生儿红细胞免疫功能探讨

本文利用红细胞Ｃ３ｂ受体花环（ＲＢＣ－Ｃ３ｂＲＲ）与红细胞免疫复合物花环（ＲＢＣ－ＩＣＲ）试验对１００例围生期新生儿红细胞免疫功能进行测定。发现出生第１天围生期儿ＲＢＣ－Ｃ３ｂＲＲ明显增高，第２天开始下降并持续稳定至第７天，低出生体重儿ＲＢＣ－ＩＣＲ增高；分娩时情况不能使红细胞免疫功能发生改变。     

黄精多糖对哮喘患儿红细胞免疫功能影响的体外实验研究

目的　观察黄精多糖 (PSP)对哮喘患儿红细胞免疫功能的影响及其意义。方法　采用花环试验法对哮喘患儿 (包括PSP组和生理盐水组 )及健康儿童进行红细胞C3b受体花环率及免疫复合物 (IC)花环率检测。结果　①哮喘组红细胞C3b受体花环率明显低于正常对照组 (t =6 .32 ,P <0 .0 1) ,IC花环率明显高于正常对照组 (t =5 .89,P <0 .0 1) ;②体外试验PSP组哮喘患儿红细胞C3b受体花环率较生理盐水组明显升高 (t =6 .33,P <0 .0 1) ,而IC花环率两组差异无显著性 (t =1.38,P >0 .0 5 ) ;③PSP在一定浓度范围内均可显著增加哮喘患儿红细胞C3b受体花环率且呈剂量依赖关系。结论　哮喘患儿红细胞免疫功能明显低下 ,PSP可增强哮喘患儿的红细胞免疫功能 ,这是其预防哮喘复发的机制之一     

黄精多糖对哮喘患儿红细胞免疫功能影响的体外实验研究

目的:观察黄精多糖(PSP)对哮喘患儿红细胞免疫功能的影响及其意义。方法：采用花环试验法对哮喘患儿(包括PSP组和生理盐水组)及健康儿童进行红细胞C3b受体花环率及免疫复合物(IC)花环率检测。结果：①哮喘组红细胞C3b受体花环率明显低于正常对照组(t=6.32,P<0.01),IC花环率明显高于正常对照组(t=5.89,P<0.01);②体外试验PSP组哮喘患儿红细胞C3b受体花环率较生理盐水组明显升高(t=6.33,P<0.01),而IC花环率两组差异无显著性(t=1.38,P>0.05);③PSP在一定浓度范围内均可显著增加哮喘患儿红细胞C3b受体花环率且呈剂量依赖关系。结论：哮喘患儿红细胞免疫功能明显低下,PSP可增强哮喘患儿的红细胞免疫功能,这是其预防哮喘复发的机制之一。     

新生儿溶血病患儿血清胆红素水平与致敏红细胞比率关系的研究

目的研究新生儿溶血病患儿血清胆红素水平与致敏红细胞比率之间的相关性。方法收集105例新生儿溶血病患儿血液标本,其中ABO溶血99例,非ABO溶血6例,采用流式细胞仪检测患儿体内被相应抗体致敏的阳性红细胞比率,同时检测其血清胆红素水平并分析比较。结果ABO溶血组致敏红细胞比率在8.2%~35.6%之间,中位数为20.5%,平均22.9%,其血清总胆红素水平在251.2μmol/L~451.1μmol/L之间,中位数为341.5μmol/L,平均352.2μmol/L;非ABO系统溶血组致敏红细胞比率在66.1%~99.5%之间,中位数为89.5%,平均88.3%;其血清总胆红素水平为在385.5μmol/L~668.9μmol/L之间,中位数为510.6μmol/L,平均527.2μmol/L。患儿血清胆红素水平随致敏红细胞比率的增高而增高。结论新生儿溶血病患儿血清胆红素水平与致敏红细胞比率具有正相关性。     

缩宫素的剂量及浓度对新生儿血清胆红素的影响及临床意义

目的探讨缩宫素的剂量及浓度对新生儿血清胆红素的影响。方法对100例足月顺产的新生儿产前使用不同剂量及浓度的缩宫素，于出生后72h观察其血清胆红素的变化。结果缩宫素的剂量越大，浓度越高，新生儿血清总胆红素的值越高。结论缩宫素的剂量及浓度对新生儿黄疸有明显影响。     

胆红素对新生儿脐血单核细胞吞噬功能的影响

目的 探讨胆红素对脐血单核细胞(CBMC)吞噬功能的影响.方法 采用纤维连接蛋白结合法分离新生儿CBMC,在含有不同浓度胆红素(102.6 μmol/L、153.9 μmol/L、220.6μmol/L和307.μmol/L)的牛血清白蛋白溶液中孵育1 h,再给予脂多糖(LPS,1μg/m1)刺激48 h,收集细胞.通过荧光标记的葡聚糖吞噬试验检测细胞的吞噬功能.结果 LPS单独作用可以增强CBMC的吞噬功能;低浓度胆红素单独作用亦可增强CBMC的吞噬功能.先经过低浓度(102.6μmol/L)胆红素孵育,再接受LPS的刺激,CBMC的吞噬功能增强,而较高浓度和高浓度胆红素(153.9、220.6、307.8 μmol/L)对CBMC的吞噬功能则起抑制作用.结论 较高浓度和高浓度胆红素可以明显抑制CBMC的吞噬功能,此种抑制作用可能与胆红素抑制细胞表面分子的表达和胆红素引起细胞凋亡的增多有关.     

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??Patients with thalassemia who received red blood cell transfusion repeatedly are more likely to produce allogeneic antibodies and autoantibodies against erythrocytes due to long-term exposure to erythrocyte membrane antigens other than ABO/Rh??D??. Antibodies can lead to difficulty in blood cross matching??comprimised efficacy of blood transfusion??hemolysis??which sometimes is life-threatening. In order to reduce allogeneic antibodies after transfusion??the range of erythrocyte cross matching should be expanded to Rh and Mia/Mur system and precision erythrocyte cross matching method should be developed for thalassemia major. For those who have clinically significant erythrocyte antibodies??corresponding antigens should be screened before transfusion??even after those antibodies become undetectable. In patients with hemolysis and shortened blood transfusion interval??autoimmune hemolytic anemia should be taken into consideration??and glucocorticosteroid-based immunosuppression therapy is recommended.     

Exploring the relationship of peripheral total bilirubin,red blood cell,and hemoglobin with blood pressure during childhood and adolescence

Objective

Total bilirubin is beneficial for protecting cardiovascular diseases in adults. The authors aimed to investigate the association of total bilirubin, red blood cell, and hemoglobin levels with the prevalence of high blood pressure in children and adolescents.

血清胆红素浓度对足月新生儿神经心理发育的影响

由于新生儿胆红素代谢特点,约50%~60%的足月儿于出生后2~3d内均出现黄疸[1],对患儿多数没有大的影响。但当血清胆红素浓度增高超过一定限度时可能影响患儿的神经心理发育,严重者可引起脑瘫[2]。但多大浓度会对新生儿中枢神经系统产生影响,其功能障碍是否随高胆红素血症治愈而恢     

胆红素对新生儿脐血单核细胞凋亡的影响

Objective To explore the effects of bilirubin on the apoptosis index(AI) of cord blood monocyte(CBMC).Methods The CBMC of normal full-term neonates selected randomly as subjects was isolated by the method of gelatin/plasma coated flasks.The CBMC was preincubated with unconjugated bilirubin dissolved in bovine albumin solution at various concentration(6 mg/dl、9 mg/dl、 12.9 mg/dl and 18 mg/dl).Bilirubin-treated CBMC was further cultured with LPS for inducing cellular activation for 48 hours,and then the CBMC was collected.The AI of CBMC after cultivation was detected by using TUNEL methods.Results No differences of AI among the normal control group,LPS group and 6 mg/dl (free-LPS) group(P ＞ 0.05) were found.In the presence of LPS,the AI of the 6 mg/dl group was similar to those of the normal control group,LPS group and 6 mg/dl(free-LPS) group(P ＞0.05),while the AIs of the 9 mg/dl group,12 mg/dl group and 18 mg/dl group were significantly higher than those of the normal control group,LPS group,6 mg/dl(free-LPS) group and 6 mg/dl group(P＜0.05).The increase of the AI of CBMC was corresponding to the raise of the bilirubin concentration.Conclusion The bilirubin can increase the AI of CBMC by destroing the configuration of the cell membrane,increasing intracellular calcium concentration and inducing the mitochondrial energy failure.     

胆红素对新生儿脐血单核细胞凋亡的影响

Objective To explore the effects of bilirubin on the apoptosis index(AI) of cord blood monocyte(CBMC).Methods The CBMC of normal full-term neonates selected randomly as subjects was isolated by the method of gelatin/plasma coated flasks.The CBMC was preincubated with unconjugated bilirubin dissolved in bovine albumin solution at various concentration(6 mg/dl、9 mg/dl、 12.9 mg/dl and 18 mg/dl).Bilirubin-treated CBMC was further cultured with LPS for inducing cellular activation for 48 hours,and then the CBMC was collected.The AI of CBMC after cultivation was detected by using TUNEL methods.Results No differences of AI among the normal control group,LPS group and 6 mg/dl (free-LPS) group(P ＞ 0.05) were found.In the presence of LPS,the AI of the 6 mg/dl group was similar to those of the normal control group,LPS group and 6 mg/dl(free-LPS) group(P ＞0.05),while the AIs of the 9 mg/dl group,12 mg/dl group and 18 mg/dl group were significantly higher than those of the normal control group,LPS group,6 mg/dl(free-LPS) group and 6 mg/dl group(P＜0.05).The increase of the AI of CBMC was corresponding to the raise of the bilirubin concentration.Conclusion The bilirubin can increase the AI of CBMC by destroing the configuration of the cell membrane,increasing intracellular calcium concentration and inducing the mitochondrial energy failure.