妇女良恶性肿瘤患者血清三种肿瘤标志物检测

选择１２５例正常健康妇女，８２例妇女良性肿瘤，７８例乳腺癌，６８例子宫颈癌和子宫体癌，５０例卵巢恶性肿瘤患者，用酶联免疫吸附法检测血清中癌胚抗原和铁蛋白。用聚丙烯酰胺凝胶电泳法分析血清乳酸脱氢酶同工酶结果：单项检测ＣＥＡ，铁蛋白和ＬＤＨ比值的阳性率分别为乳腺癌４５％〉６２％和５８％：子宫吕和子宫体癌５０％，５８％和６１％；卵巢恶性肿瘤６７％，６８％和７９％。     

血清α－L－岩藻糖苷酶及糖类抗原CA－50对原发性肝细胞癌诊断的探讨

对７８例原发性肝细胞癌（ＨＣＣ）、１５例其他消化道恶性肿瘤，１９例肝硬化、５例肝血管用及健康对照３０例，同时检测α－Ｌ－岩藻糖苷酶（ＡＦＵ）、糖类抗原ＣＡ－５０并与甲胎蛋白（ＡＦＰ）对照。结果：ＡＦＵ、ＣＡ－５０对ＨＣＣ诊断的阳性率分别为７６．２％和６９．２％高于ＡＦＰ（６２．８％）。２７例ＡＦＰ阴性的ＨＣＣ中，ＡＦＵ、ＣＡ－５０呈阳性者分别为１７和９例。三者联合检测诊断的特异性为８５．２％，敏感性达９１．１％。１６例术后检测结果，ＡＦＵ阴转率为８７．５％，ＣＡ－５０为８１．３％。ＡＦＵ和ＣＡ－５０做为诊断ＨＣＣ的标志物，对ＡＦＰ阴性者有互补诊断价值，联合检测诊断率更高。     

P53蛋白在喉上皮癌前病变及癌组织中的表达及其意义

为探讨喉上皮癌变过程中Ｐ５３蛋白表达水平,采用免疫组化技术检测了１１例喉上皮单纯性增生（ＳＨＦ）、３２例非黄型性增生（ＡＨＥ）及４２例喉鳞状细胞癌（ＬＳＣＣ）Ｐ５３蛋白的表达,结果：Ｐ５３蛋白阳性表达率在ＳＨＥ、ＡＨＥ及ＬＳＣＣ中分别为０．００％、３１．２５％及５２。３８％;轻、中、重度ＡＨＥ阳性率分别为０．００％、３３．３３％及７５％;高、中、低分化ＬＳＣＣ阳性率分别为６２．５０％、７６．９２％     

CEA,CA19—9和CA—50联合检测在胃肠道癌诊断中的作用

研究１２８例胃肠道恶性肿瘤患者及１２０例胃肠道良性病变患者的血清癌胚抗原（ＣＥＡ），糖类抗原（ＣＡ）ＣＡ１９－９和ＣＡ－５０水平。结果表明：恶性肿瘤组，三种肿瘤标志物的阳性率分别为ＣＥＡ５４．７％，ＣＡ１９－９ ５５．５％，ＣＡ－５０ ４６．９％，联合检测阳性率达７７．３％。胃肠道良性疾患三类肿瘤抗原的假阳性率分别为ＣＥＡ８．７％，ＣＡ１９－９ ４．２％，ＣＡ－５０ ５．０％。恶性肿瘤组与良性病变     

CA125对妇科肿瘤的诊断价值

测定１３６例妇科肿瘤术后血清ＣＡ１２５值，结果表明，卵巢恶性肿瘤阳性率为１００％，ＣＡ１２５为３６９．４９±７３．１５ＫＵ／Ｌ，子宫内膜异位症阳性率６１．３％，ＣＡ１２５为７１．０７±３３．６３ｋＵ／Ｌ，子宫肌瘤阳性率１６．７％，ＣＡ１２５为２７．２２±１５．５３ｋＵ／Ｌ，卵巢良性肿瘤阳性率为１６．７％．ＣＡ１２５为２５．５０±１５．７５ｋＵ／Ｌ，正常对照组ＣＡ１２５为１８．４２±８．３５ｋＵ／Ｌ。认为ＣＡ１２５为卵巢恶性肿瘤早期诊断、疗效观察、肿瘤复发的灵敏度指标。     

CA125、LDH在卵巢癌中的辅助诊断意义

选取卵巢上皮癌４０例、宫颈癌１３例、宫内膜癌１３例、良性肿瘤５０例和正常健康妇女５０例，以探讨卵巢上皮细胞癌膜抗原（ＣＡ１２５）、乳酸脱氢酶（ＬＤＨ）及同工酶、Ｃ－反应蛋白（ＣＲ）、唾液酸（ＳＡ）辅助诊断卵巢上皮癌的意义。结果：正常组血清ＣＡ１２５值（４×１０３±１．７６×１０３ｕ／Ｌ）及良性肿瘤组ＣＡ１２５值（１５×１０３±８．２４×１０３ｕ／Ｌ）明显低于卵巢癌组（２８０×１０３±７５×１０３ｕ／Ｌ）（Ｐ＜０．０５）；ＣＡ１２５辅助诊断卵巢上皮癌的敏感性（ＳＳ）为９５％、阳性拟然比（ＰＬＲ）为４．７５、准确性（ＡＣ）为８６％，明显高于宫颈癌的ＳＳ（８５％）、ＰＬＲ（４．２５）、ＡＣ（８０％）及宫内膜癌的ＳＳ（７７％）、ＰＬＲ（３．８５）、ＡＣ（７９％）。Ⅲ、Ⅳ期卵巢癌的ＣＡ１２５值（３９０×１０３±８２×１０３ｕ／Ｌ）明显高于Ⅰ、Ⅱ期卵巢癌的ＣＡ１２５值（２１０×１０３±７５×１０３ｕ／Ｌ）（Ｐ＜０．０５）；ＣＡ１２５的ＳＳ、ＮＰＶ、ＡＣ明显高于ＬＤＨ、ＣＲ、ＳＡ、ＣＲ的ＳＳ、ＮＰＶ、ＡＣ（Ｐ＜０．０５）；ＣＡ１２５的ＳＳ明显高于ＬＤＨ同功酶的ＳＳ７７％（Ｐ＜０．０５）。结论：ＣＡ１２５辅助诊断卵巢上     

血清α—L—岩藻糖苷酶及糖类抗原CA—50对原发性肝细胞癌诊断…

对７８例原发性肝细胞癌，１５例其他消化道恶性肿瘤，１９例肝硬化，５例肝血管瘤及健康对照３０例，同时检测α－Ｌ－岩藻糖苷酶，糖类抗原ＣＡ－５０并与甲胎蛋白对照。结果：ＡＦＵ，ＣＡ－５０对ＨＣＣ诊断的阳性主分别为７６．２％和６９．２％高于ＡＦＰ。     

评多项肿瘤标记物对卵巢癌的诊断价值

目的 为了提高卵巢恶性肿瘤早期诊断的敏感性、特异性及病情监测的效率,筛选出较敏感的肿瘤标记物及联合检测方案。方法 检测了５０例卵巢恶性肿瘤、３０例卵巢良性肿瘤及２５例正常妇女血清中ＣＡ１５３、ＣＡ１２５、ＬＳＡ、ＴＳＧＦ含量。结果 单项检测的诊断效率从大至小依次为ＣＡ１２５、ＬＳＡ、ＴＳＧＦ、ＣＡ１５３,联合方案中以ＣＡ１２５＋ＴＳＧＦ诊断效率最大。临床Ⅰ～Ⅱ期患者ＣＡ１２５＋ＴＳＧＦ的敏感性及特     

P53蛋白在喉上皮癌前病变及癌组织中的表达及其意义

为探讨喉上皮癌变过程中 Ｐ５３ 蛋白表达水平,采用免疫组化技术检测了１１ 例喉上皮单纯性增生（ Ｓ Ｈ Ｅ）、３２ 例非典型性增生（ Ａ Ｈ Ｅ）及 ４２ 例喉鳞状细胞癌（ Ｌ Ｓ Ｃ Ｃ） Ｐ５３ 蛋白的表达。结果： Ｐ５３ 蛋白阳性表达率在 Ｓ Ｈ Ｅ、 Ａ Ｈ Ｅ 及 Ｌ Ｓ Ｃ Ｃ 中分别为 ０００％ 、３１．２５％ 及 ５２．３８％ ;轻、中、重度 Ａ Ｈ Ｅ 阳性率分别为 ０００％ 、３３．３３％ 及７５％ ;高、中、低分化 Ｌ Ｓ Ｃ Ｃ阳性率分别为６２．５０％ 、７６．９２％ 、１５．３９％ ,其差异有高度显著性（ Ｐ＜ ０．０１）。 Ｐ５３ 蛋白表达与喉癌临床分期、分型、颈淋巴转移、局部复发及预后无相关性。结果提示：喉上皮由正常状态向恶性转化的过程中,存在抑癌基因突变产物 Ｐ５３ 蛋白过度表达,可能与喉癌的发生发展有一定关系。     

癌胚抗原与乳酸脱氢酶对良恶性胸腔积液的鉴别诊断价值

目的：探讨胸腔积液及血清癌胚抗原（ＣＥＡ）及乳酸脱氢酶（ＬＤＨ）对良恶性胸腔积液的鉴别诊断价值。方法：分别采用双抗体放射免疫法和速率法检测１８例结核性和３２例癌性胸腔积液及血清ＣＥＡ和ＬＤＨ水平。结果：结核性及癌性胸腔积液、血清ＬＤＨ值及二者比值（ＰＬＤＨ／ＳＬＤＨ）均无显著性差异（Ｐ〉０．０５）。结核性及癌性胸腔积液、血清ＣＥＡ值及二者比值（ＰＣＥＡ／ＳＣＥＡ）均具有显著性差异（Ｐ〈０．０１）。     

Detection by monoclonal antibody of carbohydrate antigen CA 50 in serum of patients with carcinoma

A solid phase radioimmunoassay was devised for measuring the value of the carcinoma associated carbohydrate antigen CA 50 in serum based on the use of a specific monoclonal antibody (C 50). Samples of serum from 259 patients with carcinoma, 114 patients with other malignancies or inflammatory diseases, and 150 healthy controls were examined. Serum values of CA 50 exceeding the mean plus three standard deviations for control samples from blood donors were found in a high proportion of patients with colorectal adenocarcinomas (50% of those with early, localised tumours and 75% of advanced cases), other gastrointestinal carcinomas (69%), uterine cancer (75% of those with corporeal and 88% of those with cervical cancer), prostatic cancer (90%), lung cancer (52%), and breast, ovarian, kidney, and urinary bladder carcinoma (26-67%). The CA 50 values in samples from patients with inflammatory diseases, including ulcerative colitis, with rare exceptions (0-7%) were within the normal range, as were those in patients with various sarcomas and malignant melanoma. Measuring serum values of CA 50, which is evidently a generalised carcinoma associated antigen, may be useful in clinical research studies of the diagnosis, management, and prognosis of patients with different types of carcinoma.     

血清CA125检测对恶性肿瘤诊断价值的探讨

目的:检测血清中糖类抗原125(CA125)含量,探讨其在恶性肿瘤诊断中的应用价值。方法:检测分析618例恶性肿瘤患者(其中卵巢癌112例、肺癌82例、肝癌89例、乳腺癌102例、胃癌81例、食管癌78例、宫颈癌74例,均经病理确诊)治疗前血清CA125含量以及同期健康体检者56例(对照组)血清CA125含量。结果:恶性肿瘤患者总体阳性率32.84%,与正常对照组(阳性率1.79%)比较差异具有显著性(P<0.01);卵巢癌患者血清CA125阳性率65.18%,其均值和阳性率与正常对照组和其他恶性肿瘤组比较差异具有显著性(P<0.01);肺癌、肝癌、乳腺癌、胃癌、食管癌、宫颈癌患者血清CA125均值和阳性率与正常对照组比较也有显著性差异(P<0.05);结论:血清CA125检测对卵巢癌应用价值较大,对肺癌、肝癌、乳腺癌、胃癌、食管癌、宫颈癌也有一定的参考价值。     

Metastatic ovarian squamous cell carcinoma

Ovarian squamous cell carcinoma is usually associated with germ cell tumours (dermoid cyst) or endometriosis in primary cancer. While tumour metastasis to the ovary is common and often bilateral in over 50 percent of cases, metastatic cervical squamous cell carcinoma to the ovary is infrequent compared to adenocarcinoma from other extraovarian primaries and the cervix. We report two cases of unilateral metastatic ovarian squamous cell carcinoma from the uterine cervix in two women aged 38 years and 48 years, respectively. They presented with abdominopelvic masses, clinically thought to be tuberculosis and primary ovarian tumour, respectively. Both had laparotomy which revealed multinodular ovarian masses with extensive extra-ovarian involvement of the corpus and uterine cervix by tumour and omental seedlings. Tissue microscopy showed total replacement of ovarian stroma by tumour with necrotic foci and containing infiltrating nests and cords of malignant squamous cells with prominent intercellular bridges. No evidence of teratoma or endometriosis was seen in the histology sections. They were both diagnosed with metastatic ovarian squamous cell carcinoma with advanced stage disease primary in the uterine cervix. Ovarian metastatic squamous cell carcinoma from the uterine cervix may occur with advanced stage cervical carcinoma. Unilateral multinodular ovarian mass with extensive extra-ovarian tumour involvement should raise suspicion of metastasis rather than of primary tumour. Early and prompt diagnosis is desirable in the management of these patients.     

94853例恶性肿瘤住院患者疾病构成及死因分析

目的：分析肿瘤住院患者的疾病构成及主要死亡原因。方法：回顾性分析2002年至2012年四川省肿瘤医院94853例肿瘤住院患者的临床资料。结果：2002年至2012年恶性肿瘤住院患者前10位的疾病类型分别为肺癌、宫颈癌、食管癌、乳腺癌、结直肠癌、鼻咽癌、肝癌、胃癌、淋巴瘤、卵巢癌,占全部恶性肿瘤住院患者的82.0％。住院患者总病死率为2.7％,男性总病死率为3.4％高于女性2.0％（ P＜0.001）;2002年至2012年肿瘤住院患者男、女及总病死率均呈明显下降趋势（ P＜0.001）。恶性肿瘤死亡患者的前10位死因依次为肺癌、肝癌、结直肠癌、食管癌、胃癌、淋巴瘤、乳腺癌、胰腺癌、卵巢癌、鼻咽癌,占死亡总数的81.3％;其中,胰腺癌的病死率最高（9.6％）,鼻咽癌最低（1.08％）。不同性别死亡患者有不同的死因顺位,前10位死因顺位中,男性食管癌的病死率高于女性（ P＝0.046）,女性胃癌病死率高于男性（ P＝0.003）。不同年龄段死亡患者有不同的死因顺位,前10位死因顺位中,肺癌、肝癌、结直肠癌、乳腺癌在60岁以上人群的病死率最高（ P＜0.001）。结论：四川省肿瘤医院肿瘤住院患者的疾病构成及死因分析为肿瘤防治工作提供了依据,肺癌、宫颈癌、食管癌是我省须重点防治的肿瘤。     

A twenty-year period in mass screening for uterine cervical cancer in Fukushima Prefecture

To explore the change in age distribution of patients with uterine cervical cancer and its precursors, we analyzed 2,168,923 women in a series of mass screening for uterine cervical cancer in Fukushima Prefecture. The first examination rate was not increased over a 20-year period. During the screening period, severe dysplasia was found in 693 women, carcinoma in situ in 672 women, microinvasive carcinoma in 517 women, and frank invasive carcinoma in 421 women. The numbers of patients with frank invasive carcinoma was decreased gradually in every age group, which might reflect the effectiveness of our mass screening project. Although the detection rates of severe dysplasia, carcinoma in situ and microinvasive carcinoma have not changed in patients groups over 40 years old, the detection rates were increased in patients under the age of 39. These results may reflect a recent tendency for cervical cancer to increase in young women. It is important, therefore, to increase the screening rate for young women to prevent the progression with advanced cervical cancer.     

2010年北京市永外地区适龄妇女两癌筛查工作分析研究

目的通过对我院承担永外地区2010年适龄妇女两癌筛查工作进行分析,总结经验与不足,为今后两癌筛查工作提供借鉴。方法对两癌筛查的流程、结果等进行汇总分。结果共有3357例适龄妇女进行了宫颈癌的筛查,3105例适龄妇女进行乳腺癌筛查,其中筛出宫颈癌人数为0例,可疑病变38例;筛查出乳腺可疑病例13例,确诊乳腺癌2例。结论两癌筛查工作,是由政府为主导,多部门协作,全社会参与的妇女常见病防治模式和协作机制,对女性健康起到了促进作用,医疗机构在此项工作中发挥了重要的作用。     

B7-H4及SccAg联合检测宫颈癌的临床研究

目的：探讨B7-H4及SccAg在宫颈癌中的变化及监测的临床意义。方法：分别用双抗体夹心ABC—ELISA法检测40例宫颈癌患者血清中B7-H4及SccAg的水平,再检测治疗3个月后的水平,与50例子宫肌瘤患者血清水平对照。结果：宫颈癌患者的血清B7-H4水平（43．71±8．62）μg／L,显著高于子宫肌瘤患者的（31．61±9．84）μg／L;治疗后B7-H4（34．48±8．16）μg／L,宫颈癌患者术前血清B7-H4水平显著高于术后,差异有显著性意义（t=3．391,P〈0．05）。宫颈癌患者血清B7-H4与SccAg的检测水平呈正相关。结论：B7-H4与宫颈癌的发病有关,可能是宫颈癌较好的肿瘤标志物,宫颈癌患者的B7-H4与SccAg的血清水平呈正相关。联合检测宫颈癌患者血清B7-H4与SccAg,能够更好地辅助诊断宫颈癌,并用于观察疗效。     

国际妇产科学杂志

卵泡刺激素可作为体外受精控制性超排卵妇女卵巢反应的预测指标；印度三甲医院人流操作程序报道；与孕妇体重增加过多或不足相关的孕前、妊娠期纤维因素分析；结扎子宫动脉治疗子宫纤维瘤；对围绝经期与绝经后妇女行宫腔镜检查前阴道放置米索前列醇扩张宫颈；多普勒超声检查与血清CA125水平在诊断卵巢肿瘤中的作用；患子宫内膜癌日本妇女的主诉、患者特征与疾病预后的关系；对宫颈肿瘤4种筛查方法的比较     

妇科肿瘤患者血清白细胞介素2受体检测的临床意义

应用双抗体夹心ＥＬＩＳＡ法检测１７０例子肌瘤，子宫颈癌、卵巢癌、绒癌等患者血清白细胞介素２受体水平，结果表明，子宫颈癌、卵巢癌及绒癌患者血清ｓＩＬ－２Ｒ水平明显高于子宫肌瘤及疾病对照组。     

卵骒癌免诊的初步研究

Ovarian tumor associated antigen was isolated from human serous cystadenocarcinomas of ovary and purified by using affinity chromatography columns coupled to anti-normal female serum and anti-normal ovarian tissue gamma globulin. Antiserum was prepared from New Zealand white rabbits. The antiserum was absorbed with normal female serum and extracts of normal ovarian tissue thoroughly. The absorbed antiserum was appraised by immunodiffusion, immunoelectrophoresis and counter-immunoelectrophoresis with intensifiers. The absorbed antiserum has a specific immune precipitin with extracts of ovarian tumor tissue. Counter-immunoelectrophoresis with intensifiers was used to detect the ovarian tumor associated antigen in the tissue and serum of various patients. Twenty-eight of 40 ovarian tumor extracts and 1 of 5 cervical carcinoma extracts showed the presence of the cancer antigen. 14/40 were positive in the sera of ovarian tumor patients and 6/10 positive in the sera of ovarian serous cystadenocarcinoma patients; 1/5 positive in the sera of cervical carcinoma patients and 2/10 positive in the sera of ovarian cysts patients. No positive result for the sera of normal females was noted. The specificity, sensitivity and accuracy of the method were 94.7%, 42.3% and 83.3%, respectively.