T_3对大鼠心肌肌球蛋白重链基因表达调控作用的研究

从克山病病区粮和非病区粮及常规饲料饲养的大鼠心肌中提取总ＲＮＡ，采用斑点印迹法分析其中的α和β－心肌肌球蛋白重链（ＣＭＩＩＣ）ｍＲＮＡ含量的变化并测定动物血清及心肌组织的Ｔ３、Ｔ４含量。结果表明，病区粮喂养大鼠心肌中α－ＣＭＨＣｍＲＮＡ含量降低，而β－ＣＭＨＣｍＲＮＡ含量升高；该组动物心肌和血清中Ｔ３含量显著下降，Ｔ４在血清中增加而在心肌中变化不大。向病区粮喂养大鼠腹腔注射Ｔ３（２μｇ／１００ｇ体重·ｄ，连续２次）观察大鼠心肌中ＣＭＨＣ基因表达及甲状腺激素水平的变化。给予外源Ｔ３后，动物心肌中α－ＣＭＨＣｍＲＮＡ明显上升，β－ＣＭＨＣ显著下降，提示在克山病病区粮饲养动物，Ｔ３对其心肌ＣＭＨＣ基因表达具有调控作用。     

T3对大鼠心肌肌肌球蛋白重链基因表达调控作用的研究

从克山病病区粮和非病区粮及常规饲料饲养的大鼠心肌中提取的ＲＮＡ，采用斑眯印迹法分析其中的α和β－心肌肌球蛋白重链（ＣＭＨＣ）ｍＲＮＡ含量的变化胶测定动物血清及心肌组织的Ｔ３、Ｔ４含量。结果表明，病区粮喂养大鼠心肌中α－ＣＭＨＣｍＲＮＡ含量降低，而β－ＣＭＨＣｍＲＮＡ含量升高；该组动物心肌和血清中Ｔ３含量显著下降、Ｔ４在血清中增加而在心肌中变化不大。向病区粮喂养大鼠腹腔Ｔ３观察大鼠心肌中ＣＭＨＣ基因     

克山病致病因素对大鼠心肌线粒体α－磷酸甘油脱氢酶的影响

测定了克山病病区粮喂养大鼠心肌线粒体α－磷酸甘油脱氢酶（ａ－ＧＰＤ）活性，并观察了硒和维生素Ｅ对该酶的影响。结果表明，病区粮组大鼠心肌线粒体α－ＧＰＤ活力明显低于非病区粮组和常规．食组，并在补０．１μｇ／ｇ＋８００μｇ／ｇＶＥ后，α－ＧＰＤ活性升高与病区粮组比较有显著差异、本实验结果提示：１、饲克山病病区粮大鼠心肌线粒体α－ＧＰＤ活力的降低，此时的心肌可能处于甲状腺激素功能低下状态．２、硒（０．１μｇ／ｇ）＋维生素Ｅ（８００μｇ／ｇ）的补充可以升高该酶活性，改善甲状腺激素功能不足状态。     

硒碘缺乏对大鼠心肌甲状腺激素代谢和α－磷酸甘油脱氢酶活性的影响

用缺Ｓｅ缺Ｉ的克山病病区粮为基础，分别添加Ｓｅ或／和Ｉ的饲料喂养４组大鼠。测定了心肌组织Ｓｅ和Ｔ３水平，α－ＧＰＤ、ＩＤＩ、ＧＰｘ活力以及血浆Ｔ４、Ｔ３水平。结果发现，缺Ｉ使大鼠血浆Ｔ４显著下降，而缺Ｓｅ又使适Ｉ大鼠血浆Ｔ４水平增高。所有４组血浆Ｔ３水平波动幅度不大。心肌未能测出ＩＤＩ活性，心肌Ｓｅ含量因饲料加Ｓｅ而增高，但没有影响心肌Ｔ３水平。心肌α－ＧＰＤ活性缺Ｉ组明显低于加Ｉ组，并与血浆Ｔ４水平高度正相关，而与心肌ＧＰｘ活性关系不明显。研究提示，克山病病区粮引起的大鼠心肌低能量代谢特点与缺Ｉ或缺Ｉ引起的ＴＨ代谢异常有明显关系。     

克山病区粮养动物心肌T45‘—脱单碘酶活力的改变及硒和维生…

本文测定了饲克山病区糖大鼠心肌Ｔ４５＇－脱单磺酶活性，结果表明病区糖组动物心肌脱碘酶活力明显低于非病区糖组，并在补硒或维生素Ｅ后升高。提示心肌组织脱碘酶的损伤所致心肌组织甲状腺激素代谢改变在克山病心肌病变中可能起着重要的作用。     

山东省克山病病区粮食致豚鼠心肌病变的实验研究

应用山东省克山病病区粮，模拟克山病高发年膳食组成喂养豚鼠。３个月后描记其心电图，测血Ｓｅ含量和ＧＳＨ－Ｐｘ活性，取心肌组织行病理学检查。结果实验动物血Ｓｅ，ＧＳＨ－Ｐ降低，心电图出现心肌缺血，损伤表现，心肌呈现灶状环死的形态学改变。表明克山病病区粮可使豚鼠发生心肌病变。本文就此种心肌病变的形成及与克山病的关系进行了讨论。     

内皮素在克山病心肌坏死发生中的作用

用放免分析方法，对饲予克山病病区粮大鼠血浆和心肌内皮素水平进行测定。结果表明，病区粮组大鼠ＥＴ水平明显升高，并且伴有全血、心肌ＧＳＨ－Ｐｘ和红细胞，心肌Ｃｕ、Ｚｎ、ＳＯＤ活力下降，血清和心肌ＬＰＯ含量增高。结果提示；ＥＴ水平的变化可能作为一种中间环节参与克山病的发病机制；ＥＴ升高可能是使克山病的心肌坏死具有缺血缺氧特征的一个重要因素。     

铬对克山病病区粮喂养大鼠的甲状腺激素及cAMP水平的影响

为探讨铬对心肌损伤时Ｔ３、Ｔ４及ｃＡＭＰ水平的影响，应用克山病病区低硒粮、加铬、加硒及加铬加硒喂养大鼠，以放射免疫法测定血清Ｔ３、Ｔ４及心肌ｃＡＭＰ含量。结果显示病区粮组Ｔ３、Ｔ４及ｃＡＭＰ水平较对照组下降（Ｐ〈０．０１），加铬、加硒及加铬加硒组较对照组无差异（Ｐ〉０．０５），较病区粮组升高（Ｐ〈０．０５￣０．０１）。铬、硒能维持低硒心肌损伤时甲状腺激素及ｃＡＭＰ的正常代谢。     

硒碘缺乏对大鼠心肌甲状腺激素代谢和α—磷酸甘油脱氢酶？…

用缺Ｓｅ缺Ｉ的克山病病区粮为基础，分别添加Ｓｅ或和Ｉ的饲料喂养４组大鼠。测定了民肌组织Ｓｅ和Ｔ３水平，α－ＧＰＤ、ＩＤＩ、ＧＰｘ活力以及血浆Ｔ４、Ｔ３水平。结果发现，缺Ｉ使大鼠血浆Ｔ４显著下降，而缺Ｓｅ又使适Ｉ大鼠血浆Ｔ４水平增高。所有４组血浆Ｔ３水平波动幅度不大。心肌未能测出ＩＤＩ活性，心肌Ｓｅ含量因饲料加Ｓｅ而增高，但没有影响心肌Ｔ３水平。心肌α－ＧＰＤ活性缺Ｉ组明显低地加Ｉ组，并与血浆Ｔ４水     

克山病区粮养动物心肌T_45~′—脱单碘酶活力的改变及硒和维生素E对其保护作用的研究

本文测定了饲克山病区粮大鼠心肌T_45′—脱单碘酶活性,结果表明病区粮组动物心肌脱碘酶活力明显低于非病区粮组,并在补硒或维生素E后升高。提示心肌组织脱碘酶的损伤所致心肌组织甲状腺激素代谢改变在克山病心肌病变中可能起着重要的作用。     

Studies on the clinical and bacteriological effects of ofloxacin on the typhoid patients and carriers

Fundamental and clinical studies of OFLX were performed against the patients with typhoid fever and typhoid carriers. 1) Clinical and bacteriological effects: Eight patients with typhoid fever and 3 typhoid carriers were treated with OFLX. Daily doses of the agent were 900 mg in 5 adult patients, 600 mg in a child patient and 3 adult carriers. In one case of the remaining 2 adult patients, daily doses of the agent changed from 800 mg to 1200 mg and from 900 mg to 1200 mg in the other one. The duration of the treatment was 9, 14 or 21 days. Clinical efficacies of OFLX against the patients proved 4 cases were "excellent", 3 cases were "good" and one case was "poor". The eradication of Salmonella typhi recognized in all cases containing 3 carriers with the exception of the "poor" case. Adverse reactions were observed transiently in 3 patients, such a slight decrease of RBC count, decrease of granulocyte count and elevation of GPT value respectively. 2) Antimicrobial activity: MICs of OFLX against 40 strains of S. typhi were 0.05 micrograms/ml and 0.1 micrograms/ml. The MICs of NFLX, CPFX and T-3262 were almost the same as that of OFLX, and those of ENX, NY-198 and NA were higher than that of OFLX. The peaks of MIC of CP and ABPC, first choice drug against typhoid fever, were 1.56 micrograms/ml and 0.38 micrograms/ml respectively. 3) Serum concentration; Serum concentrations of OFLX were serially measured on 5 patients through the day. The concentrations of the drug were distributed from 0.82 micrograms/ml to 6.34 micrograms/ml at 6.30 a.m. and from 2.52 micrograms/ml to 11.2 micrograms/ml at 9:00 p.m. Those of the day time showed considerable individual differences.     

An essay on the strength of the heart and on the effect of aging upon it

How best to express the strength of the heart or the effort which goes into its contraction presents a problem. Certainly “work” in the Newtonian sense is not the answer; and another solution, the tension-time, is suggested; its derivatives may also be required. Most measurements of the various aspects of cardiac function which can be expressed quantitatively, such as cardiac output, slope of ascending pulse-wave front and ballistocardiogram, indicate that cardiac function diminishes at a rate of between 0.5 to 2 per cent a year as age advances. There is an apparent exception to this rule, because estimates of cardiac strength based on the classic method, the pulse amplitude, suggest that cardiac strength increases as age advances. An explanation for this apparent discrepancy is advanced. In addition to diminishing in strength, there is an increased tendency for the heart's contraction to become more incoordinate as age advances.     

Effect of estradiol on the progesterone receptor and on morphological ultrastructures in the fetal and newborn uterus and ovary of the rat

The effect provoked by estradiol after administration to pregnant rats (1 mg per day) was studied in fetal and newborn uteri and ovary. Estrogen receptors are found in the fetuses of non-treated animals. Their number (in fmol/mg DNA, +/- SD) in the fetal uterus (total sites, cytosol + nuclei) was at the age of 18 days: 63 +/- 15; at 20 days 101 +/- 13; and in the 24-h-old newborn; 415 +/- 120. The respective values in the ovary were: 105 +/- 25; 520 +/- 60 and 410 +/- 190. Estradiol stimulated significantly the progesterone receptor in the fetal uterus at 20 days old. The progesterone receptor (in fmol/mg DNA, +/- SD) which was 97 +/- 17 in the non-treated animals, increased to 790 +/- 90 in the E2-primed animals. Newborns, 24-h-old, had no detectable progesterone receptor, but in the E2-treated animals the value increased to 1210 +/- 120. In the fetal ovary of non-treated animals, progesterone receptor at the age of 18 days is: 90 +/- 19; at 20 days 132 +/- 47, and in the newborns 260 +/- 67; in the E2-treated animals, the values are respectively 330 +/- 49; 865 +/- 78 and 1280 +/- 307. In the fetal uteri of E2-treated animals, histological and ultrastructural studies showed an increase in the size of the uterine horn, the height of the epithelial cells, and stromal cell differentiation. It is suggested that, as was extensively demonstrated in the fetal compartment of the guinea-pig, the machinery for estrogen responses operates also during fetal development of the rat.