重组人胸腺素α原体外对IFN-γ,IFN-α及TNF-α的影响

目的体外观察重组人胸腺素α原(prothymosin α,Pro Tα)对几种重要细胞因子分泌的影响。方法用脾淋巴细胞、脾巨噬细胞及腹腔巨噬细胞,以ELISA法检测Pro Tα对IFN-γ,IFN-α和TNF-α分泌的影响。结果1×10^-7 mol·L^-1 Pro Tα明显促进脾细胞分泌IFN-γ(P<0.05),该浓度的Pro Tα也明显刺激小鼠脾巨噬细胞分泌IFN-α(P<0.01);在小鼠腹腔巨噬细胞中,Pro Tα能明显刺激IFN-α和TNF-α的分泌(P<0.01)。结论Pro Tα对细胞因子IFN-γ,IFN-α和TNF-α的分泌均有促进作用。     

重组人胸腺素α原体外对IFN-γ,IFN-α及TNF-α的影响

目的　体外观察重组人胸腺素α原 (prothymosinα ,ProTα)对几种重要细胞因子分泌的影响。方法　用脾淋巴细胞、脾巨噬细胞及腹腔巨噬细胞 ,以ELISA法检测ProTα对IFN γ ,IFN α和TNF α分泌的影响。结果　 1× 10 - 7mol·L- 1 ProTα明显促进脾细胞分泌IFN γ(P <0 0 5 ) ,该浓度的ProTα也明显刺激小鼠脾巨噬细胞分泌IFN α(P <0 0 1) ;在小鼠腹腔巨噬细胞中 ,ProTα能明显刺激IFN α和TNF α的分泌 (P <0 0 1)。结论　ProTα对细胞因子IFN γ ,IFN α和TNF α的分泌均有促进作用。     

急性髓系白血病患者血清IL-12,IFN-γ,VEGF检测的意义

目的 :探讨急性髓系白血病患者血清白细胞介素_12(IL_12)、干扰素_γ(IFN_γ)和血管内皮生长因子(VEGF)的变化及其意义。方法 :应用酶联免疫法测定急性髓系白血病患者化疗前后血清IL_12、IFN_γ和VEGF的水平 ,并与正常对照组比较。结果 :化疗前急性髓系白血病患者血清IL_12、IFN_γ低于对照组 (P<0.01) ,VEGF高于对照组 (P<0.05) ;IL_12与IFN_γ水平呈正相关(P<0.01) ,VEGF与IL_12、IFN_γ水平无直线相关性 ;化疗后达完全缓解时血清IL_12、IFN_γ水平升高 ,VEGF水平下降 ,与正常对照组差别无统计学意义。结论 :急性髓系白血病患者血清IL_12、IFN_γ和VEGF的变化可能与白血病细胞的生长及机体抗肿瘤免疫功能受损有关     

Plasma IL-2, NK, IFN-γ, and C3 in male workers exposed to traffic pollutants

The aim of the study is to evaluate if the occupational exposure to urban stressors could cause alterations in interleukin-2 (IL-2), NK, interferon-γ (IFN-γ) and C3 plasma levels in male traffic police officers compared to controls. After excluding the principal confounding factors, 108 traffic police officers were matched with 108 controls by age, working life, habitual consumption of alcohol and spirits. IL-2 mean levels were significantly higher in traffic police officers compared to controls (p=0.04). The distribution of IL-2 values in traffic police officers and in controls was significant (p=0.01). The distribution of NK value percentage in traffic police officers and in controls was significant (p=0.000). IFN-γ and C3 mean levels were not significant in traffic police officers compared to controls. Our results suggest that the occupational chronic exposure to low doses of urban stressors could affect NK and IL-2 plasma concentrations in traffic police officers of male sex.     

IFN-γ转基因与重组IFN-γ注射对结肠癌肝转移小鼠的治疗作用

γ干扰素（IFN-γ）可提高肿瘤细胞表面抗原的表达和激发宿主抗肿瘤免疫应答反应,在肿瘤的治疗中发挥重要作用。本研究采用经腹腔注入脂质体载体将huIFN-7基因转染荷瘤小鼠体内,通过转基因体内表达huIFN-γ了解是否可以发挥抑制肿瘤的作用,并与外源性给予重组huIFN-γ两者治疗效果相比较,报道如下。     

重组人胸腺素α原体外对IFN-γ、IFN-α及TNF-α的影响

目的　在体外实验中考察克隆并表达的人胸腺素α原 (prothymosinα ,ProTα)对几种重要细胞因子IFN γ、IFN α和TNF α分泌的影响。方法　在体外分离脾淋巴细胞、脾巨噬细胞及腹腔巨噬细胞 ,用ELISA法检测ProTα对IFN γ、IFN α和TNF α分泌的影响。结果　 10 - 7mol·L- 1 ProTα明显促进脾细胞分泌IFN γ(P <0 .0 5 ) ,该浓度的ProTα也明显刺激小鼠脾巨噬细胞分泌IFN α(P <0 .0 1) ;在小鼠腹腔巨噬细胞中 ,ProTα能明显刺激IFN α和TNF α的分泌 (P <0 .0 1)。结论　在体外实验中 ,ProTα对细胞因子IFN γ、IFN α和TNF α的分泌均有促进作用 ,这为ProTα进一步体内研究奠定了基础     

Influenza A virus replication is inhibited in IFN-λ2 and IFN-λ3 transfected or stimulated cells

Interferons lambda (IFN-λ) are the most recently defined members of the class III cytokine family. To investigate whether IFN-λ2 and IFN-λ3 displayed antiviral activity against influenza A virus (IAV), a number of cell lines induced with IFNs - as well as two established cell lines (A549-IFN-λ2 and A549-IFN-λ3) - were infected with IAV. Our results indicate that IFN-λ2 has statistically significant antiviral activity in A549-IFN-λ2 (P=0.0028) although less so than IFN-λ3, which reduced viral titer to 10% (P<0.0001). The reverse was observed for cells treated with IFNs, with IFN-λ2-treated A549 cells inhibiting IAV infection more efficiently than IFN-λ3-treated A549 cells. The antiviral effect on IFN-stimulated cells was most apparent on Vero cells (compared with MDCK and HeLa). Both IFNs significantly inhibited IAV replication and inhibition was observed in a dose-dependent manner, with an optimal IFN concentration of 20 ng/ml. IFN-λ2 was more potent than IFN-λ3 on Vero cells while IFN-λ3 appeared more efficient than IFN-λ2 on MDCK and HeLa cells.     

羧甲基茯苓多糖对人外周血淋巴细胞分泌IFN-α和IFN-γ的调节作用及中间体制备

用羧甲基茯苓多糖（CMP）预处理人外周血淋巴细胞（HPBL），经200IU／mlIFN－α启动后，加NDV的促诱生组的α-干扰素（IFN－α）效价比无CMP的常规诱生组高1．2倍；经PHA和／或ConA促诱生组的γ-干扰素（IFN－γ）效价比无CMP的PHA和／或ConA刺激常规诱生组高0．5倍（P＜0.01），尤以CMP＋PHA＋ConA促诱生的IFN－γ效果最佳。说明CMP具有IFN－α和IFN－γ促诱生效应（P＜0．01）。用CMP促诱生的IFN－α和IFN－γ中间体经各项指标检测不仅IFN－α和IFN－γ效价高，而且可达到生物制剂药用标准，经临床试验证明安全有效。     

大鼠脑缺血后IFN-γ mRNA的表达

目的探讨大鼠脑缺血后,干扰素-γ(IFN-γ)是否参与免疫损伤和IFN-γ在脑缺血中的作用。方法用线拴封闭大脑中动脉的方法制作脑缺血动物模型,采用原位杂交的方法检测缺血脑组织IFN-γmRNA表达动态的变化。结果(1)缺血脑半球和假手术的脑半球相比较,在1h时IFN-γmRNA的数量就显著增加(P<0.001),且随着脑缺血时间延长IFN-γ表达量增加,6hP<0.001,12hP<0.01,24hP<0.001,48hP<0.001,6dP<0.001。(2)IFN-γmRNA表达数量损伤面积扩大而增加;结论在脑缺血过程中IFN-γmRNA表达增加,IFN-γmRNA表达主要参与大脑损伤后期反应过程。     

人重组IFN-α对巨噬细胞的调节作用

本文报告了人重组和杂交的α干扰素(IFN-α)对巨噬细胞介导的杀肿瘤活性有调节作用。人IFN-α-A/D可抑制小鼠IFN-γ激活小鼠腹腔巨噬细胞的杀肿瘤活性,而人IFN-α-A、-D和-A/D均可激活人单个核细胞的杀肿瘤活性。作者用小鼠腹腔巨噬细胞和健康人外周血单个核细胞作为效应细胞,分别用小鼠B16-F10肿瘤细胞和人黑素瘤A375细胞株作为靶细胞。效应细胞加IFN-γ和微量脂多糖(作激活效应细胞的第二信号)等处理18～24小时后,加入~(125)I UdR标记的靶细胞,效应     

IFN-Ⅰ抗病毒效应信号通路研究新进展

IFN-Ⅰ作为一种重要的抗病毒药物在临床上日益受到关注,其抗病毒效应的发挥主要通过IFN-Ⅰ及随后的多种抗病毒分子合成,这两步的实现均需依托相应信号通路的信号转导。近年来,人们对几条 IFN-Ⅰ相关的抗病毒信号通路及其相应的调控机制已有深入了解。此文就IFN-Ⅰ主要抗病毒信号通路及其调控机制的最新进展做一综述。     

Effects of carbamazepine and metabolites on IL-2, IL-5, IL-6, IL-10 and IFN-γ secretion in epileptic patients: the influence of co-medication

Carbamazepine is a widely used anticonvulsive agent. Its metabolic pathway leads not only to the major active metabolite, carbamazepine-10,11-epoxide, but also to minor terminal metabolites such as iminostilbene and acridine. Carbamazepine is usually well-tolerated, but it may lead to rare, but serious, hypersensitive reactions associated with hypereosinophilia. The mechanisms of hypersensitivity reactions to carbamazepine are still largely unknown, and the implications of the cell-mediated immune response (Th1 pathway) or the humoral immune response (Th2 pathway) are still not understood in these reactions. It is also unclear whether the parent drug or its subsequent metabolites are the primary trigger agent. In our study, we performed ex vivo experiments to evaluate the stimulation of cytokine secretion by carbamazepine, carbamazepine-10,11-epoxide, iminostilbene and acridine. IL-5, IL-6 and IL-10 were quantified as markers of the Th2 pathway, and IL-2 and IFN-γ were used as markers of the Th1 pathway. Blood samples (n=24) were obtained from epileptic patients routinely treated with carbamazepine alone or co-treated with lamotrigine or valproate. The concentrations of cytokines in the plasma were determined before and after 3 h stimulation with drugs. We found a significantly positive effect of co-treatment with valproate on the basal level of IL-5 (p<0.01) and IL-10 (p<0.05). IL-5 production increased only in blood stimulated with a high level of acridine (33 μM), whereas IL-6 production was less specifically stimulated (p<0.05). Because IL-5 is the most potent stimulating factor of the eosinophils, we suggest that the potential helper effect of valproate and acridine can lead to hypersensitive reactions to carbamazepine in the context of the humoral immune response.     

重组IFN-α 2a治疗癌症的临床研究

对20例播散性癌症病人进行α2a干扰素(IFN-α2a)治疗的Ⅰ期临床研究表明,一次肌肉注射18×10~6～100×10~6uIFN-α2a,可出现发热(39～40℃)、流感样症状(头痛、寒战和乏力等)、胃肠道症状(厌食、呕吐和恶心等)和指、趾麻木等副反应,这些症状与IFN剂量无关,通常在24～72小时内消失。一些病人出现与剂量相关的白细胞减少、血小板减少及血清GOT、GPT、乳酸脱氢酶(LDH)和肌酐值升高,除白细胞数需2～4周恢复     

雾化吸入IFN-α治疗病毒性疾病的研究进展

目的探索IFN-α(IFN-α)在非典(SARS)、中东呼吸综合征(MERS)和儿童病毒性疾病中的作用,为IFN-α治疗新型冠状病毒肺炎(COVID-19)的临床研究提供一些可借鉴的思路。方法通过检索国内外数据库,获取与IFN-α相关的数据。结果目前尚缺乏IFN-α治疗NCP临床研究数据,IFN-α对SRAS的疗效尚未肯定,对MERS的最佳治疗方法仍未达成共识。但在儿童病毒性疾病中,雾化吸入IFN-α具有作用迅速、疗效可靠、用药剂量小、全身不良反应少等优点,在我国儿童中广泛应用。结论目前尚无NCP的特效药,IFN-α对新冠肺炎的疗效和安全性需要更进一步的临床研究。     

重组人IFN-γ的抗巨细胞病毒作用

在某些情况下人类巨细胞病毒感染(HCMV)可能极为严重,但目前尚无有效的治疗方法。大剂量干扰素(IFN)在体外可抑制HCMV生长,而在临床试治中则未见明显疗效。作者研究了重组人IFN-γ(rIFN-γ)对HCMV复制的影响,并与IFN-α进行了比较。所用的细胞株为人胚成纤维细胞(HEF)和人羊膜细胞(FL);两株病毒为HCMV AD169     

IFN-γ刺激对大鼠脑神经细胞的影响

目的探讨IFN-γ刺激对大鼠脑神经细胞的相关变化的影响。方法采用IFN-γ刺激大鼠脑神经细胞株,刺激后24h,取细胞对神经细胞表达MHC进行检测。结果过高浓度的IFN-γ将对神经细胞有毒害作用,适量浓度的IFN-γ对神经细胞MHC表达有促进作用。结论 IFN-γ对神经细胞有双重作用,既有毒害作用又有促进作用,其主要与其用量有明显的相关性。     

外周血单个核细胞分泌Th细胞因子IFN-γ,IL-10在原因不明反复流产患者的研究

目的探讨原因不明反复流产妇女外周血单个核细胞分泌Th细胞因子。方法采用细胞分离,混合淋巴细胞培养实验和用酶联免疫吸附法检测20例反复自然流产患者血液中的分泌Th细胞因子水平,并以20例正常健康早孕组为对照。结果反复自然流产患者IFN-γ高于对照组,IL-10低于对照组数据经统计学比较差异有统计学意义（P〈0.05）。结论反复自然流产患者的Th的分泌的IFN-γ,IL-10细胞因子失衡可能是反复自然流产的原因之一。     

IL-17及IFN-γ在溃疡性结肠炎中的表达

目的：检测Th17类细胞因子IL-17和Th1类细胞因子IFN-γ在溃疡性结肠炎（UC）患者中的表达,以此来探讨溃疡性结肠炎的发病机制。方法：用ELISA法分别检测溃疡性结肠炎患者和正常对照组结肠组织及血单核细胞中IL-17和IFN-γ的表达。结果：UC组结肠组织中IL-17表达较正常对照组显著增加（P〈0.05）;而UC组结肠组织IFN-γ表达较正常对照组无显著增加（P〉0.05）;UC组血单核细胞IL-17、IFN-γ表达较正常对照组无显著差异（P〉0.05）。结论：局部IL-17升高在UC的发病中可能起重要作用。     

不同亚型IFN-α对STAT1表达的影响

目的用实时荧光定量聚合酶链反应(FQ-PCR)方法检测不同亚型干扰素α(IFN-α2b、IFN-α2a、IFN-α1b)对HepG2细胞内信号传导分子STAT1mRNA表达的影响。方法1000IU/mlIFN-α2b、IFN-α2a、IFN-α1b分别作用于HepG2细胞4、8、16、24h后,用RT-PCR的方法扩增STAT1目的片段,用T-A克隆方法构建定量的标准模板,并用荧光定量PCR方法观察IFN-α2b、IFN-α2a、IFN-α1b作用后HepG2细胞内STAT1mRNA的表达水平。结果经IFN-α2b、IFN-α2a、IFN-α1b处理后,HepG2细胞内的STAT1mRNA水平较未用IFN-α的空白对照组明显上调,并于IFN-α处理8h后STAT1mRNA水平达高峰。此时,IFN-α2b、IFN-α2a和IFN-α1b的STAT1mRNA水平(×107copies/ml)依次为3·59±0·25、2·73±0·43和4·85±0·55,三者之间存在显著性差异。结论IFN-α作用于HepG2细胞后,IFN-α1bSTAT1mRNA的表达水平最高,IFN-α2b次之,IFN-α2a最弱。间接说明IFN-α1b的抗HBV活性较强,IFN-α2b次之,IFN-α2a较弱。