重组Der f1变应原诱导小鼠哮喘模型的建立

目的建立重组粉尘螨Ⅰ类变应原（Der f1）诱导BALB/c小鼠哮喘模型。方法 30只BALB/c小鼠随机分成3组：PBS阴性对照组、卵清蛋白（OVA）阳性对照组、重组Der f1模型组。用酶联免疫吸附试验（ELISA）检测小鼠血清中IgG1、IgG2α和IgE含量,ELISA法检测脾细胞培养上清液和支气管肺泡灌洗液（BALF）IL-2、IL-4、IL-5、IL-17和IFN-γ含量,计数BALF中细胞总数,同时切取小鼠未灌洗侧肺组织进行病理学观察。结果 OVA阳性对照组、重组Der f1模型组小鼠血清中IgG1、IgG2a和IgE含量,脾细胞上清液和BALF中IL-2、IL-4、IL-5、IL-17和IFN-γ含量与PBS阴性对照组相比差异均具有统计学意义（P〈0.05或P〈0.01）;OVA阳性对照组、重组Der f1模型组小鼠BALF中细胞总数和嗜酸性粒细胞（EOS）计数显著高于PBS组（P〈0.05或P〈0.01）。OVA阳性对照组和重组Der f1模型组小鼠肺部病理改变呈明显的变态反应性炎症。结论用重组Der f1能成功诱导BALB/c小鼠哮喘模型。     

尘螨Ⅱ类改组变应原对哮喘小鼠免疫治疗的效果

目的 探讨以改组尘螨Ⅱ类变应原基因Der f2和Der p2后表达的蛋白为变应原,对小鼠哮喘模型的特异性免疫治疗效果。方法 随机将160只清洁级BALB/c小鼠分为PBS组（阴性对照组）,Der f2和Der p2免疫治疗组（阳性对照组）,哮喘组,改组变应原免疫治疗M01、M03、M08、M10蛋白治疗组。用尘螨提取液于0、7、14d腹腔注射致敏激发BALB/c小鼠,第21天雾化吸入激发,连续7d,其中各免疫治疗组于第25～27天雾化前30min分别用Der f2、Der p2和改组变应原进行特异性免疫治疗,PBS组则用PBS进行腹腔注射和雾化吸入。最后1次雾化24h后,引颈处死。分别观察肺组织病理变化、支气管肺泡灌洗液（BLAF）白细胞计数,酶联免疫吸附试验（ELISA）测定BLAF和脾细胞培养上清液细胞因子IL-4、IL-17和IFN γ的含量及血清中特异性IgG1、IgE抗体水平变化。结果 与哮喘组比较,改组变应原免疫治疗组和阳性治疗组肺部炎症显著减轻, BALF中的总细胞数及嗜酸性粒细胞数,血清中抗原特异性IgG1、IgE抗体均显著降低;免疫治疗组（包括阳性对照）的BALF和脾细胞培养上清液中的细胞因子IL-4、IL-17均明显降低,IFN-γ含量显著升高。结论 通过基因改组获得的尘螨Ⅱ类变应原改组疫苗免疫治疗小鼠哮喘,可有效降低尘螨引起的小鼠肺部炎症。     

Immune responses against Schistosoma japonicum after vaccinating mice with a multivalent DNA vaccine encoding integrated membrane protein Sj23 and cytokine interleukin-12

Background The vaccination of mice with DNA encoding single candidate antigens has failed to induce significant protection against Schistosoma japonicum ( S. japonicum) challenge infections. In this study, we evaluated the feasibility of using a multivalent DNA vaccine which co-expressed S.japonicum integral membrane protein Sj23 and murine cytokine IL-12 to induce protective immune responses.Methods The plasmid pVIVO2-IL12-Sj23, a eukaryotic expression vector expressing Sj23 and murine IL-12 simultaneously, was constructed, identified, and tested for expression in vitro. Its ability to protect against S. japonicum challenge infections was analyed according to worm reduction rate and egg reduction rate after vaccination of BALB/c mice. The serum levels of specific IgG antibody were determined by enzyme-linked-immuno sorbent assay (ELISA) and Western blot analysis. Using cultured spleen cells, IFN-γ and IL-4 post-stimulation were quantified by ELISA. The phenotypes of splenocyte populations were analyzed by flow cytometry (FCM).Results The plasmid DNA pVIVO2-IL12-Sj23 was proven to express well in vitro by transient transfection of HEK-293 cells. Immunization resulted in a worm reduction rate of 45. 53% and egg reduction rate of 58.35%. ELISA and Western blot analysis indicated that immunized mice generated specific IgG against Sj23. Spleen cells showed significant increases in IFN-γ but decreases in IL-4.No significant differences in CD4^ and CD8^ subgroup ratios were observed after the challenges.Conclusions The multivalent DNA vaccine pVIVO2-1L12-Sj23 is sufficient to elicit moderate but highly significant levels of protective immunity against challenge infections. Cytokine IL-12, as a gene adjuvant, was able to enhance the Thl responses and, hence, the protective immunity.     

白介素2基因佐剂协同单纯疱疹病毒1型糖蛋白D核酸疫苗免疫诱导的特异性免疫应答

目的研究白介素2(IL-2)cDNA协同单纯疱疹病毒1型(HSV-1)gD核酸疫苗免疫对机体体液免疫和细胞免疫应答的影响,以及在HSV-1病毒角膜攻击时的保护效果.方法利用pcDNA3.1载体分别构建HSV-1糖蛋白D和IL-2的真核表达质粒pgD和pIL-2,体外鉴定其表达.动物实验分为pcDNA3.1空载体对照组、pgD单独免疫组和pgD pIL-2联合免疫组3组,具体为通过肌肉免疫接种BALB/c小鼠3次,间隔2周,每次接种质粒100μg,第3次免疫后2周,用酶联免疫吸附试验(ELISA)检测抗体滴度并做亚型分析,利用3H-TdR掺人法进行Th细胞增殖实验,ELISA试剂盒检测Th细胞分泌的IL-2、IFN-γ和IL-10水平,耳廓肿胀实验检测迟发型超敏反应(DTH)反应;HSV-1病毒角膜攻击后,裂隙灯显微镜观察角膜上皮病变.结果与pgD单独免疫组相比,pIL-2的协同免疫可以显著提高IgG2a抗体滴度、Th细胞增殖反应和DTH反应,Th细胞分泌IL-2和IFN-γ水平也显著提高,IL-10水平明显下降.在动物保护实验中,pIL-2的协同免疫明显增强了pgD疫苗在病毒攻击时对角膜的保护效果.结论IL-2 cDNA的协同免疫可以显著提高pgD核酸疫苗诱导的体液免疫和细胞免疫应答水平,增加核酸疫苗的免疫保护效果.     

Der p2重组BCG对哮喘小鼠气道炎症的抑制作用

目的：观察胞壁型屋尘螨抗原Derp2重组卡介苗（Derp2rBCG）对哮喘小鼠气道炎症的抑制作用.方法：实验分4组,小鼠分别给予生理盐水、BCG或Derp2rBCG处理,然后以Derp2为变应原建立哮喘模型,正常对照组给予生理盐水致敏及气道内滴入,取肺泡灌洗液、肺组织标本,观察肺部炎症细胞浸润、气道杯状细胞增殖及肥大细胞脱颗粒情况.结果：①Derp2rBCG下调了哮喘小鼠气道局部炎症;②Derp2rBCG减轻了哮喘小鼠肺组织中的炎症细胞浸润;③Derp2rBCG可以减轻哮喘小鼠气道粘液高分泌现象并下调杯状细胞数量;④Derp2rBCG减轻了哮喘小鼠气道中的肥大细胞脱颗粒现象.结论：Derp2rBCG是一种有效的哮喘免疫治疗方法.     

Protective effect of DNA- mediated immunization with a combination of SAG1 and IL- 2 gene adjuvant against infection of Toxoplasma gondii in mice

Objective To characterize the immune response induced by SAG1 encoding plasmid combined with IL- 2 gene adjuvant in mice and to assess the protective effect of this vaccination against toxoplasmosis. Methods Mice were co- injected intramuscularly with plasmid encoding Toxoplasma gondii SAG1 plus murine IL- 2 expression vector at a dose of 100 μg. Booster immunizations were employed 2 more times at 3- week interval. As controls, mice were inoculated with PBS or empty plasmid pcDNA3. Humoral and cellular responses were assayed using ELISA for the determination of Ab, Ab isotype and IFN- γ, as well as IL- 4. To detect the integration and dissemination of DNA in the injected mice, PCR and in situ hybridization were performed. All mice were then infected with highly virulent RH tachyzoites of Toxoplasma gondii intraperitoneally. Results Significant increases in specific IgG levels were observed in mice after immunization three times with SAG1 expression plasmid. With respect to the IgG isotype, co- inoculation of IL- 2 expression plasmid enhanced the level of IgG2a and the production of IFN- γ. Challenging mice by vaccinating with combined plasmids with RH tachyzoites resulted in prolonged survival. Conclusion Humoral and cytokine responses elicited by SAG1 DNA immunization can be modulated by co- inoculation with IL- 2 expression plasmid. The use of DNA vaccine in combination with an appropriate cytokine gene to prevent T. gondii infection warrants further investigation.     

重组耻垢分枝杆菌经鼻粘膜免疫在小鼠体内的分布与表达

目的：探讨携带编码人天然颗粒溶素（GLS）和小鼠IL-12基因质粒（pZM03）的重组耻垢分枝杆菌经鼻粘膜免疫在小鼠体内的分布与表达。方法：将携带绿色荧光蛋白（GFP）基因质粒（pUV15）的重组耻垢分枝杆菌滴鼻BALB／c小鼠后2周，处死小鼠，观察肺、脾组织中荧光蛋白和耻垢分枝杆菌的分布；将携带GLS和IL-12质粒（pZM03）的重组耻垢分枝杆菌滴鼻免疫BALB／C小鼠3次，第1次免疫后4周，处死小鼠，用免疫组化检测肺、脾组织中GLS表达，用ELISA检测血清IL-12和肺泡灌洗液特异性SIgA的水平。结果：在BALB／c小鼠肺、脾组织中均检测到绿色荧光蛋白和耻垢分枝杆菌的分布，以及GLS的表达，并有血清IL-12水平升高和粘膜特异性SIgA的产生。结论：携带GFP的重组耻垢分枝杆菌可在肺和脾组织分布；携带GLS和IL-12的重组耻垢分枝杆菌经鼻粘膜免疫小鼠后，可引起呼吸道粘膜特异性抗菌免疫，以及GLS和IL-12的体内表达，为新型疫苗的研究奠定了基础.     

尘螨主要变应原蛋白的IgE结合表位序列分析

摘要：目的对屋尘螨主要变应原（Der p 1）蛋白分子中与过敏病人血清特异性IgE相结合的表位序列的鉴定。进而获得
基于串联表位的小分子低毒过敏原以作为新的免疫治疗剂。方法采用全蛋白序列重叠扫描法对Der p 1蛋白进行全表
位筛选,即合成了覆盖Der p 1全蛋白222个氨基酸的31段各含15个氨基酸的多肽,且相邻肽段间有8个氨基酸的重叠。
并将这些肽段按点状固相多肽合成法依顺序合成于纤维膜上。再将该膜与由数份过敏血清组成的血清池孵育,经抗人
IgE-HRP二抗结合及X光片显影后对阳性点进行灰度比对及分析。结果经对X光片阳性点的比对及分析,我们确定出
了Der p 1过敏原蛋白中的3个强阳性表位序列。它们是位于第85~99位的氨基酸序列（表位1,Ep1）,第106~120位的氨
基酸序列（表位2,Ep2）及第190~204位的氨基酸序列（表位3,Ep3）。结论我们获得了Der p 1中3个15肽的线性IgE结
合表位（B细胞表位）序列。证实了Der p 1分子中存在IgE结合的线性表位。为进一步构建T/B细胞串联表位的小分子低
毒过敏原提供了物质基础。     

IgE PRODUCTION IS INVOLVED IN BUTYRATE-ENHANCED NK CELL ACTIVITY IN VIVO

It has been demonstrated that patients with asthma have a large number of NK cells and show a stronger NK activity. These results indicate that NK cell activity may be related to total IgE level in serum in healthy subjects.Previously, we have found that sodium butyrate (NaBu) markedly enhanced the IL-4-induced IgE production in the LPS-stimulated murine splenocytes in vitro, and inductive rat IgE production in vivo, and enhanced the NK cell activity ex vivo. We hypothesized that the IgE production might be involved in butyrate-enhanced NK cell activity in vivo. Mice were intraperitoneally treated/immunized with NaBu or/and Ascaris suum extract (ASC), and thespleen NK cell activity was evaluated. Furthermore, the effect of serum (NAS) on IL-2- or IFN-γ-induced spleenNK cell activity was determined. The spleen NK cell activity and IL-2- or IFN-γ-induced spleen NK cell activity of mice treated/immunizedwith NaBu or/and ASC were stronger than those of untreated/unimmunized mice. Although IL-4 blocked IL-2(100 U/ml)- or IFN-γ,( 100 U/ml)-induced increase in NK cell activity, these NK cell activities in mice treated/immunized with NaBu/ASC were not inhibited. IgE production showed a tendency to rise in NaBu-treatedmice serum, and a synergistic effect was observed with treatment of NaBu and ASC. Moreover, the NAS significantly increased IL-2(25 U/ml)-or IFN-γ-(25 U/ml)-induced NK cell activity, and its effect was inhibited by an-ti-mouse IgE mAb. These data show that IgE plays an important role in NAS-enhanced IL-2/IFN-γ-induced NK cell activity, andIL-4 does not inhibit IgE and IL-2/IFN-γ-induced NK cell activity in mice.     

Immunity induced by DNA vaccine of plasmid encoding the rhoptry protein 1 gene combined with the genetic adjuvant of pcIFN-γ against Toxoplasma gondii in mice

Objective To construct the eukaryotic expression recombinant plasmid, pcIFN-γ, as a gene tic adjuvant and observe the immune responses elicited by pcDNA3-rhoptry protei n 1 （pc-ROP1） combined with pcIFN-γ against Toxoplasma gondii (T.go ndii) infection in mice.Methods A fragment of the IFN-γ gene was directly inserted into the pcDNA3 plasmid and identified by two restriction endonucleases digestion.pcIFN and pcROP1 DNA wa s injected into the left leg muscle of mice at a dosage of 100 μg, and a boost er vaccination was given at the same dosage after two weeks.Control groups wer e injected with pcDNA3 blank plasmid or normal saline.At 30, 50 and 70 days af ter booster injection, kinetic tests were carried out: MTT assay for the prolife ration response of T lymphocyte cells and the activity of NK cells, sandwich ABC -ELISA for the determination of IFN-γ, IL-2 and IL-10; a serum enzymetic aa ssay for nitric oxide (NO) in sera and ELISA for the titer of IgG antibody in sera.Results The recombinant plasmid, pcIFN-γ was constructed.The proliferation response of spleen T lymph cells, NK cell killing activity, and serum levels of IFN- γ, IL-2 and NO in mice injected with pcROP1 and pcIFN-γ were higher than in those injected with pcROP1 alone.There was no difference in IgG antibody level s between the two groups. Conclusion The genetic adjuvant, pcIFN-γ, could enhance the cellular immune response indu ced by DNA vaccine of pcROP1 in mice against Toxoplasma gondii infection.     

支气管哮喘患者血清免疫球蛋白E和支气管肺泡灌洗液中白介素8测定及意义

目的探讨免疫球蛋白E(IgE)和白介素8(IL-8)在支气管哮喘发病机制中的作用以及在诊疗中的意义。方法选择25例支气管哮喘急性发作患者为哮喘组,另选正常健康体检者16例为对照组。采集所有研究对象血清和支气管肺泡灌洗液(BALF),使用多抗体夹心酶联免疫吸附方法检测2组血清IgE和BALF中IL-8含量,比较2组血清IgE和BALF中IL-8含量,并进行相关性分析。结果哮喘组血清IgE水平和BALF中IL-8水平均较对照组明显增高(P<0.01);哮喘组血清IgE和BALF中IL-8水平之间呈显著正相关(r=0.73,P<0.01)。对照组血清IgE和BALF中IL-8水平之间无明显相关性(r=0.26,P>0.05)。结论IL-8和IgE可能参与了哮喘的发病过程,测定二者的水平,对临床诊断支气管哮喘有一定的应用价值。     

Immunogenicity of interleukin 12 and DNA vaccine prime-BCG boost against Mycobacterium tuberculosis

OBJECTIVE: To investigate the immunogenicity of vaccine strategies about human interleukin 12 associated with combined DNA (Ag85A and ESAT-6) prime-BCG boost. METHODS: BALB/c mice were divided into PBS negative control and 4 immunity groups: BCG group, DNA/BCG group, DNA + IL-12/BCG group and DNA/BCG + IL-12 group. All mice received three immunizations at 2-week interval. Specific IgG antibody in serum of mice was determined with indirect ELISA in 4, 6, 8 weeks respectively after final vaccination. The splenic lymphocytes of mice were separated and stimulated with PPD to measure their proliferation by flow cytometry, and to evaluate the production of interferon-gamma (IFN-gamma) in cell suspensions of spleen cells by ELISA. The levels of CD4+ and CD8+ T-cell on surface of spleens lymphocyte were determined by flow cytometry. RESULTS: PPD could stimulate specific IgG responses in 4 immunity groups, and the average valences of 4 groups are 1:80, 1:120,1:160,1:160; the splenic lymphocyte proliferation reactions and IFN-gamma production were detectable in 4 immunity groups, and the most significant response occurred in 12 weeks. DNA + IL-12/BCG group and DNA/ BCG+IL-12 group induced higher production than BCG group and DNA/BCG group (P < 0.05), and the effects between DNA + IL-12/BCG group and DNA/BCG + IL-12 group had little difference. The numbers of CD4+ and CD8+ T-cell in 4 immunity groups were much higher than PBS group (P < 0.05), and DNA+IL-12/BCG group and DNA/BCG+IL-12 group were detected much more CD4+ and CD8+ T-cell than BCG group and DNA/BCG group (P < 0.05). The level of T-cell between DNA + IL-12/BCG group and DNA/BCG + IL-12 group had little difference. CONCLUSION: Interleukin 12 associated with the strategy of priming with the combined DNA vaccines and boosting with attenuated M. bovis vaccine (BCG) could induce much stronger specific cellular immunity compared with simple DNA/BCG or attenuated M. bovis vaccine alone.     

融合基因GM-CSF/IL-2转基因瘤苗在肝癌特异性免疫治疗中的应用

目的 制备人白细胞介素2(hIL-2)与鼠粒-单核细胞集落刺激因子(mGM-CSF)融合基因修饰的H22肝癌全细胞瘤苗,探索融合基因GM.CSF/IL-2转基因瘤苗,在肝癌主动免疫治疗中特异性抗肿瘤作用.方法 用含hlL-2与mGM-CSF融合基因的真核表达载体在体外转染H22肝癌细胞,制成疫苗,皮下接种Balb/c小鼠,同时建立Balb/c小鼠荷瘤模型,ELISA法检测H22/pcDNA3.1( ).GM-CSF/IL-2瘤苗免疫组小鼠与各对照组小鼠(分别接种H22/pcDNA3.1( )瘤苗、H22瘤苗、PBS)血清中IL-10、IFN- γ水平,观察小鼠存活期;同时用51Cγ释放法测定H22/pcDNA3.1( )-GM-CSF/IL-2瘤苗免疫组小鼠与各对照组小鼠(单纯荷瘤组、正常组)脾细胞对亲本H22肝癌细胞的杀伤活性.结果 成功制备了含有hIL2与mGM-CSF融合基因的H22肝癌瘤苗,转基因瘤苗免疫组小鼠的脾细胞在体外对亲本H22肝癌细胞的杀伤率为38.3%,显著高于对照组小鼠的脾细胞对亲本H22肝癌细胞的杀伤率(分别为13.6%和7.5%),也高于其对S180细胞的杀伤率(9.1%)(P＜0.05).转基因瘤苗免疫组小鼠血清IFN-γ较对照组明显升高(P＜0.01),血清IL-10较对照组明显降低(P＜0.01).同时,转基因瘤苗免疫组小鼠生存期亦有明显延长.结论 转染hIL-2与mGM-CSF融合基因的同系肿瘤细胞瘤苗可激发特异性细胞介导的免疫反应,改善抗肿瘤免疫反应,延长荷瘤小鼠生存期.     

CD4+CD25+调节性T细胞在过敏性哮喘和无症状的过敏者中表达的差异

目的:比较过敏性哮喘患者、无症状的过敏者和不过敏的健康人外周血CD4+CD25+调节性T细胞(Treg)数量的差异及对特异性过敏原刺激的反应.方法:选取急性发作期的尘螨过敏性哮喘患者20例,无症状的尘螨过敏者24例,不过敏的健康人22例,分离外周血单个核细胞(PBMC)并进行CFSE染色,分别在不刺激和1mg/L尘螨抗...     

KGF-2对COPD小鼠保护性作用的免疫学研究

 目的  研究角质细胞生长因子2(keratinocyte growth factor-2,KGF-2)对慢性阻塞性肺病(chronic obstructive pulmonary disease,COPD)模型小鼠肺部炎症及免疫功能的影响。方法  取15只6～8 周龄(体重20～25 g)的雄性BALB/C小鼠,首先用烟熏加气道滴注脂多糖(lipopolysaccharide,LPS)的方法构建COPD模型。KGF-2组于第1天和第14天给予KGF-2,共2次。采用肺组织HE染色及肺泡灌洗液(bronchoalveolar lavage fluid,BALF)蛋白浓度评价小鼠气道炎症及肺泡壁屏障的破坏。ELISA检测小鼠血浆及BALF中IL-6和肿瘤坏死因子(tumor necrosis factor α,TNF-α)的水平。流式细胞术检测小鼠脾脏中CD4+T细胞及CD8+T细胞的比例及细胞程序性死亡蛋白1(programmed cell death protein 1,PD-1)的表达。结果  与COPD组小鼠比较,KGF-2组小鼠肺部炎症细胞浸润减少,肺泡结构相对完整,肺泡灌洗液及血浆中IL-6和TNF-α水平有降低的趋势。与COPD组比较,KGF-2组小鼠脾脏中CD4+T细胞及CD8+T细胞的比例升高(P<0.05),PD-1比例降低(P<0.05)。结论  KGF-2可能具有抑制COPD相关肺组织炎症及增强免疫功能的作用。     

百日咳蛋白对小鼠过敏性哮喘模型的免疫调节作用

目的：观察百日咳杆菌蛋白对致敏小鼠气道炎症、气道高反应性以及IFN-γ／IL-4平衡的调节作用。方法：致敏小鼠，卵白蛋白反复攻击后静脉注射乙酰甲胆碱（Mch），测定气道反应性；收集支气管肺灌洗液（BALF），检测炎症细胞及IL-4、IFN—γ水平，进行肺组织病理学检查。结果：肌注或滴鼻给予百日咳杆菌蛋白，能抑制致敏小鼠肺阻力及肺动态顺应性变化，上调肺泡灌洗液中IFN-γ／IL-4比例，降低嗜酸性粒细胞聚集，并且作用呈现剂量依赖性。病理学检查显示，百日咳杆菌蛋白能够有效抑制致敏小鼠气道上皮杯状细胞增生及肺组织中炎症细胞浸润。结论：百日咳杆菌蛋白能有效抑制过敏性哮喘小鼠肺部炎症、改善肺功能，对防治哮喘具有潜在的应用前景。     

早期接触过敏原对大鼠哮喘模型的影响

目的:了解早期接触卵蛋白(ovalbumin,OVA)对大鼠哮喘模型的影响.方法:将新生SD大鼠随机分为阴性对照组、哮喘模型组、小剂量组和大剂量组,每组8只,其中小剂量组和大剂量组大鼠于生后当天分别一次性注射2g/L OVA 0.1 mL和10g/L OVA 0.1 mL.6周后,哮喘模型组、小剂量组和大剂量组经OVA致敏并制作哮喘模型.分别观察各组大鼠肺组织病理、支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中细胞计数和分类、外周血白细胞介素-4(interleukin-4,IL-4)、γ-干扰素(interferon-γ,IFN-γ)、OVA-IgE和OVA-IgG水平.结果:小剂量组和大剂量组肺组织病理显示气道炎症较哮喘组明显减轻,BALF中细胞总数、中性粒细胞和嗜酸性粒细胞比例较哮喘组显著下降,两组大鼠血IL-4和OVA-IgE较哮喘模型组显著下降,IFN-γ水平则较哮喘模型组显著增加.大剂量组与对照组比较,IL-4和IFN-γ及OVA-IgE差异无统计学意义.哮喘模型组、小剂量组和大剂量组大鼠血OVA-IgG较对照组显著增加,且大剂量组较哮喘组显著增加.结论:出生后早期接触OVA可抑制大鼠成年后OVA所诱发的气道炎症改变和特异性IgE增加,其机制可能与诱导免疫耐受形成有关.     

日本血吸虫重组BCG-Sj26GST疫苗诱导小鼠脾细胞IL-6变化的研究

目的：研究日本血吸虫重组BCG－Si26GST疫苗对小鼠脾细胞IL－6的影响,方法：实验1采用该疫苗皮下免疫小鼠,免疫后8周用日本血吸虫尾缦进行攻击感染,感染后6周剖杀小鼠,同时设有PBS对照组,实验2用该疫苗皮下和静脉注射分免疫小鼠,于免疫后,0,4,8,10,14和16周各剖杀4只,分离脾脏,用Si26或PHA刺激脾细胞,用ELISA法检测5 清液中IL－6含量,结果：疫苗免疫尾缦攻击后,IL－6水平无明显变化;动态观察发现IL－6于疫苗免疫后8－10周达最高水平,结论：IL－6可能与日本血吸虫重组BCG－Si26GST疫苗诱导的保护性免疫力无关。