类黄酮和维生素E协同对抗细胞膜损伤的作用

目的：观察类黄酮和维生素E在拮抗壬基酚的致损伤作用方面的表现。 方法：实验于2005—03在湖南环境生物学院实验中心完成。实验动物为5月龄SD大鼠30只。①抽取大鼠的外周血0．5mL种人含植物血凝素的RPMI1640培养基培养后，按照加入试剂的不同分为4组，空白对照组【注入蒸馏水（5，10，15，20mL）】，壬基酚组【注入壬基酚（5，10，15，20mL）】，类黄酮组【注入类黄酮（1&;#215;10^-6,1&;#215;10^-5,1&;#215;10^-4mol／L）0．5mL】，维生素组[注入维生素E（0．25，0．50，1．00，2．00mg）】。不同处理后用姐妹染色体色差法制成色差法染色体玻片，镜检淋巴细胞姐妹染色单体交换率。②抽取SD大鼠的外周血种人含植物血凝素RPMI1640培养基培养。按照加入试剂的不同分5个组，壬基酚组（注入壬基酚10μL），壬基酚＋类黄酮1组（注入壬基酚10μL＋类黄酮10^-6mol／L，经温育），壬基酚＋类黄酮2组（注入壬基酚10μL＋类黄酮10^-6mol／L，不经温育），壬基酚＋维生素组（注入壬基酚10μL＋维生素E0．5mg），壬基酚＋类黄酮＋维生素E组（注入壬基酚10μL＋类黄酮10^-6moL／L＋维生素E0．5mg）。按照不同处理后用姐妹染色体色差法制成色差法染色体玻片，在荧光显微镜下观测50个细胞，镜检姐妹染色单体交换率。③取相对固定部位的肝组织块，经处理后制成超薄切片经醋酸铀-枸椽酸铅双重染色，在透射电镜下观察膜系统。 结果：经不同试剂处理培养外周血用姐妹染色体色差法制成的染色体玻片全部进入结果分析。①当每瓶加入的壬基酚达到15μL时，细胞数明显减少，即外周血淋巴细胞分裂增殖的趋势明显减缓或停滞，累积的分裂相不多。各种壬基酚剂量处理下的姐妹染色单体交换率／细胞的数值与对照组相比差异有显著性。（爹未经37℃、4h温育的100mol／L的类黄酮没有显示出对壬基酚的拮抗作用，与相应经过温育的处理组相比差异有显著性。经温育处理的10^-6mol／L的类黄酮溶液可以明显降低姐妹染色单体交换率，与对照组比较差异呈显著性（P〈0．05）。而0．5mg的维生素E对壬基酚并未呈现显著的拮抗致损伤作用。但10^-6mol／L类黄酮溶液0．5mL和维生素E0．5mg同时处理，与对照组比较细胞姐妹染色单体交换率出现大幅下降，差异呈现显著性。③透射电镜观察可见，壬基酚染毒组与拮抗物处理组比较肝细胞失去正常形态，细胞膜消失，核膜破碎，细胞胞质未见结构完整清晰的细胞器，膜结构损伤严重。 结论：类黄酮可显著降低壬基酚处理过的淋巴细胞姐妹染色体交换率，类黄酮单因素存在一定的拮抗作用。终浓度0．9&;#215;10^-7mol/L的类黄酮和90．9mg／L的维生素E在降低壬基酚处理过的淋巴细胞姐妹染色体交换率方面存在显著的协同效应。即一定剂量的类黄酮和维生素E在拮抗壬基酚的致损伤作用中存在着显著的协同效应。     

维生素C和维生素E对移植肺再灌注损伤保护作用的实验研究

目的：观察维生素C（Vit）和维生素E（VitE）对大鼠移植肺再灌注损伤的保护作用。方法：采用大鼠自体左肺模拟原位移植模型，治疗组分别于再灌注前10min静脉注入VitC（200mg/kg）和（或）VitE乳剂（100mg/kg）。再灌注4h后观测移植肺气体交换功能，顺应性，血管通透性，脂质过氧化程度，ATP含量以及组织病理学变化。结果：缺血再灌注（I／R）组移植肺气体交换功能，顺应性降低，脂质过氧化程度增加，ATP含量下降，组织病理学改变明显（P＜0．01）；给药组移植肺气体交换功能，顺应性与I／R组相比无明显改善（P＞0．05），其它指标均有不同程度的改善，以VitC和VitE合用时改善最为显著（P＜0．01）。结论：VitC和VitE对移植肺再灌注损伤具有协同保护作用。     

维生素E对心肌氧化损伤的保护作用研究

目的研究维生素E对阿霉素引起的心肌细胞过氧化损伤的保护作用。方法以原代培养的乳鼠心肌细胞建立损伤模型。在实验中,将细胞分为3组:空白对照组、模型组(2mg/L)的阿霉素与乳鼠心肌细胞共培养。药物修复组:在加入阿霉素的同时,加入维生素E(150、300、600μg/ml与心肌细胞共同培养。测定各组心肌细胞的乳酸脱氢酶I(LDHI)含量、丙二醛(MDA)含量及一氧化氮合酶(NOS)活性的变化。结果模型组心肌细胞的LDHI含量增高(P<0.01),MDA含量增加(P<0.05),NOS活性增高(P<0.01).而药物修复组使心肌细胞的LDHI含量降低(P<0.01),MDA含量降低(P<0.05),NOS活性降低(P<0.01),且降低的幅度随药物浓度的增加而增加。结论维生素E和对受损的心肌细胞有明显的保护作用。     

维生素C和维生素E对糖尿病患者肾脏的保护作用

糖尿病肾病 (ＤＮ)是糖尿病 (ＤＭ )最常见的致死致残性微血管并发症。尿白蛋白排泄率 (ＵＡＥＲ)水平升高 ,是 2型ＤＭ进展成终末阶段肾病的高危险性的标志 ,已有几项研究支持降低ＵＡＥＲ是肾脏保护的有效治疗目标[1] 。维生素Ｃ和维生素Ｅ是体内重要的抗氧化剂 ,动物试验及临床试验均已证实 ,补充药理剂量的维生素Ｃ和维生素Ｅ ,可以降低ＵＡＥＲ升高的 2型ＤＭ患者的ＵＡＥＲ[2 ] 。为进一步证实这种作用 ,我们观察了维生素Ｃ和维生素Ｅ联合治疗对 2型ＤＭ患者ＵＡＥＲ的影响。1　资料与方法1.1　对象　 96例 2型ＤＭ患者均符合 1996年…     

维生素E对鼠海马神经元细胞的抗氧化损伤作用

目的观察维生素E对老年痴呆(Alzheimer'sdisease)的治疗作用,研究氧化损伤对鼠海马神经元细胞的损伤作用及维生素E对其氧化损伤的保护作用。方法用氧化(H2O2)、维生素E处理后氧化的方法对鼠海马神经细胞(HT-22)进行分组处理。用二维电泳法、蛋白银染法及特殊氧化蛋白免疫染色法探测被氧化的蛋白。结果氧化处理12h后出现细胞生存率明显下降31.19%;经维生素E处理后再氧化处理的细胞生存率几乎达到正常组水平。经H2O2氧化处理的鼠海马神经元细胞的氧化蛋白数目增加了,而维生素E提前处理过的那组没有增加。结论维生素E对鼠神经元细胞的氧化损伤起保护作用,是有效的抗氧化治疗剂。     

维生素E抗肿瘤作用机制研究

本文就维生素E抗瘤的作用机制作一简述。     

Effect of vitamin E and vitamin C supplementation on antioxidative state and renal glomerular basement membrane thickness in diabetic kidney

The aim of this study was to analyze the effect of vitamins C and E on malondialdehyde (MDA) content and activities of key antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) as well as glomerular basement membrane (GBM) thickness in streptozotocin-induced diabetic kidney in rats. Wistar male rats were divided into following groups (12 rats each): the control, diabetic rats, diabetic rats whose drinking water was supplemented with vitamin C in a dose of 1.0 g/l or diet was supplemented with 200 mg of vitamin E/100 g fodder. Body weight, blood glucose and HbA1C levels and 24-hour urinary albumin excretion (UAE) were studied every week (0-12 weeks). After 6 and 12 weeks, MDA content and activities of SOD, CAT and GSH-Px were measured in the kidney homogenate supernatants. Electron micrographs of glomeruli were scanned and morphometric investigations were performed by means of computer image analysis system to compare GBM thickness. The blood glucose and HbA1C concentrations and UAE in diabetic rats were significantly higher than in the control group. An increase in the MDA level and decrease in the SOD, CAT and GSH-Px activities in the kidney of diabetic rats were observed after 6 and 12 weeks of experiment. Administration of vitamins C and E did not affect body weight, blood glucose and HbA1C levels. Both vitamin C and vitamin E decreased lipid peroxidation and augmented the activities of antioxidant enzymes studied in the kidneys of diabetic rats as well as reduced UAE, decreased kidney weight and GBM thickness. The results indicate the potential utility of antioxidant vitamins in the protection against the development of diabetic nephropathy.     

维生素E在大鼠肾脏缺血再灌注中的作用

目的 观察维生素E(VE)对大鼠肾脏缺血再灌注(I/R)的作用.方法 18只健康雄性Wistar大鼠随机分为假手术组、I/R组、VE+ I/R组,每组6只.所有动物于I/R术24h后处死,取肾组织进行光镜检查,留取血清测定尿素氮(BUN)和肌酐(SCr)的浓度.Western印迹测定肿瘤坏死因子α(TNF-α)蛋白的表达.结果 HE、PAS染色结果显示,I/R组肾小管及间质病理损伤显著重于假手术组,VE+ I/R组小管间质损伤程度较肾脏I/R组明显减轻.与假手术组相比,I/R组以及VE+I/R组BUN和SCr均明显升高[BUN:(10.13±2.14)、(7.67±1.63)、(3.85±0.21) mmol/L,SCr:(80.33±7.15)、(63.67±5.40)、(48.67±3.61)μmol/L](P均＜0.05);VE+ I/R组较I/R组BUN和SCr水平显著降低(P均＜0.05).Western Blotting检测显示,VE+ I/R组肾组织TNF-α蛋白表达与I/R组相比显著降低(P＜0.05).结论 VE可能通过抑制TNF-α表达发挥对I/R中肾脏保护作用.     

Protective effect of vitamin E against ethanol-induced small intestine damage in rats

The role of oxidative stress and inflammatory reaction has been reported in various ethanol-induced complications. The purpose of this study was to evaluate the effect of ethanol-induced structural alteration, oxidative stress, and inflammatory reaction on the small intestine of rats, and plausible protective effect of vitamin E to determine whether it inhibits the abnormality induced by ethanol in the small intestine. Twenty-four male wistar rats were divided into three groups, namely: Control^©, ethanol, and vitamin E treated ethanol groups.After six weeks of treatment, the small intestine length, villus height, crypt depth and muscular layer thickness, oxidative stress, and inflammatory parameters showed significant changes in the ethanol treated group compared to the control group. Vitamin E consumption along with ethanol ameliorated structural alteration of the small intestine and reduced the elevated amount of oxidative stress and inflammatory markers such as protein carbonyl, OX-LDL, IL-6, Hcy, and TNF-α. Furthermore, their total antioxidant capacity was increased significantly compared to that of the ethanol group. These findings indicate that ethanol induces the small intestine abnormality by oxidative and inflammatory stress, and that these effects can be alleviated by using vitamin E as an antioxidant and anti-inflammatory molecule.     

维生素E修饰的透析膜对血液透析患者血脂的影响

目的观察VitE修饰的透析膜对血液透析患者血脂的影响.方法 40名稳定血液透析患者随机分为2组.A组(n=20)用VitE修饰的透析膜透析;B组(n=20)用纤维素透析膜透析,观察4周.分别于第1次和第13次透析前抽血,测定血浆和红细胞中丙二醛(MDA)、VitE及血浆中的甘油三酯(TG)、总胆固醇(TCH)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)、脂蛋白A[Lp(a)]、载脂蛋白B(ApoB)、载脂蛋白A1(ApoA1)浓度.结果 A组实验结束比实验前血浆VitE(P=0.003)和红细胞中VitE(P=0.032)明显升高,血浆MDA(P=0.043)和红细胞内MDA(P=0.033)明显下降.TC、TG、LDL、HDL无明显变化,但Lp(8)(P=0.049)、ApoB(P=0.040)明显下降,ApoA1(P=0.027)较前明显升高.B组实验前后观察指标无明显变化.结论使用VitE修饰的透析膜透析有抗氧化作用,改善脂质代谢,有助于防止动脉粥样硬化.     

Influence of apolipoprotein E polymorphism on plasma vitamin A and vitamin E levels

BACKGROUND: Plasma concentrations of vitamins A and E are positively correlated with those of concurrent lipids and, on the other hand, lipid levels are influenced by apolipoprotein E polymorphism. Therefore, the effect of this polymorphism on both vitamins was analysed in an adult population. MATERIALS AND METHODS: Subjects were recruited from a working population. Their anthropometric, lifestyle and dietary intake variables and menopausal status were recorded. Their apolipoprotein E phenotype and their plasma vitamins A and E (by high-performance liquid chromatography) and lipid (enzymatically) concentrations were determined after an overnight fast. The associations of the phenotype with vitamins and lipids were studied in men and women separately and controlling for significant covariates. RESULTS: The apolipoprotein E phenotype was associated with the concentrations of total, low-density lipoprotein (LDL) and high-density lipoprotein (HDL) cholesterol in women, whereas no associations with lipids were found in men. Vitamin A and vitamin E levels were higher in men than in women, but only the difference in the former persisted after lipid adjustment. Apolipoprotein E2 slightly increased vitamin A levels in women, an effect which was still evident with lipid adjustment. Actually, both the apolipoprotein E phenotype and triglyceride were selected as significant predictors of this vitamin by multiple regression. This phenotype did not affect vitamin E levels in either sex. CONCLUSIONS: Lipids do not mediate the effect of gender on vitamin A levels. Apolipoprotein E polymorphism is an independent determinant of vitamin A levels in women. Pending confirmation by others, we propose that enhancement of this vitamin may contribute to the beneficial impact of the epsilon2 allele on human ageing and health.     

口服Vitamin E对离心运动后大鼠骨骼肌损伤的影响

目的 从骨骼肌超微结构和肌浆网自由基代谢的角度,探讨维生素E(Vitamin E)对离心运动后大鼠骨骼肌损伤的影响.方法 取健康雄性SD大鼠48只,随机分为对照组、运动组、生理盐水组、Vitamin E组,每组各12只,制作相应的动物模型.动物模型采用一次持续性下坡跑运动训练,运动结束后,取大鼠右侧肱三头肌,部分用于电镜标本制做,余下肌肉采用差速离心方法提取肌浆网,并进行丙二醛(MDA)含量和超氧化物歧化酶(SOD)活力测定.结果 Vitamin E组大鼠骨骼肌在离心运动后24 h的损伤超微结构较生理盐水组改善明显,与运动组和生理盐水组比较,丙二醛显著降低,超氧化物歧化酶显著增高,但仍差于对照组.结论 Vitamin E可以降低骨骼肌肌浆网丙二醛含量、增加肌浆网超氧化物歧化酶活性,并使离心运动后骨骼肌损伤形态学改变得以改善.