Fukiic and piscidic acid esters from the rhizome of Cimicifuga racemosa and the in vitro estrogenic activity of fukinolic acid

Hydroxycinnamic acid esters of fukiic acid and piscidic acid were isolated from a 50% ethanolic extract obtained from the rhizomes of Cimicifuga racemosa (Ranunculaceae). Besides 2-E-caffeoylfukiic acid (fukinolic acid), 2-E-feruloylfukiic acid (cimicifugic acid A), 2-E-isoferuloylfukiic acid (cimicifugic acid B), 2-E-feruloylpiscidic acid (cimicifugic acid E) and 2-E-isoferuloylpiscidic acid (cimicifugic acid F), free caffeic, ferulic and isoferulic acids were isolated. The estrogenic activity of fukinolic acid was shown by increased proliferation (126% at 5 x 10(-8) M) of an estrogen dependent MCF-7 cell system with reference to estradiol (120% at 10(-10) M).     

过山蕨中有机酸类化学成分

目的研究过山蕨(Camptosorus sibiricusRupr.)中有机酸类化学成分。方法采用反复硅胶柱层析分离纯化,通过理化常数测定和光谱分析鉴定其化学结构。结果从过山蕨中分离得到了11个有机酸类化合物,即咖啡酸(caffeic acid,1)、香豆酸(courmaric acid,2)、原儿茶酸(protocate-chuic acid,3)、对羟基苯甲酸(4 hydroxybenzoic acid,4)、异香草酸(isovanillic acid,5)、2,4二羟基苯甲酸(2,4 dihydroxybenzoic acid,6)、肉桂酸(cinnamic acid,7)、丁二酸(succinic acid,8)、棕榈酸(palmitic acid,9)、香豆酸4OβD吡喃葡萄糖苷(transpcoumaric acid 4OβDglucopyranoside,10)、咖啡酸4OβD吡喃葡萄糖苷(caffeic acid 4OβDglucopyranoside,11)。结论化合物2~11,均为首次从该属植物中分离得到。     

青钱柳地上部分的化学成分研究

从青钱柳Cyclocarya paliurus(Batal)ljinskaja.地上部分分离出14个化合物,通过NMR波谱方法和理化性质考察分别鉴定为山柰酚(1),山柰酚-3-O-α-L-吡喃鼠李糖苷(2),山柰酚-3-O-β-D-吡喃半乳糖苷(3),山柰酚-3-O-β-D-吡喃葡萄糖苷(4),山柰酚-3-O-α-L-(4′′-E-p-香豆酰基)吡喃鼠李糖苷(5),青钱柳酸B(6),阿江榄仁酸(7),2α,3β,23-三羟基-12-烯-28-乌苏酸(8),2α-羟基乌苏酸(9),齐墩果酸(10),槲皮素(11),异槲皮苷(12),β-谷甾醇(13),胡萝卜苷(14)。化合物2、3、5和8为首次从青钱柳属中分离得到。     

A norsesquiterpene lactone and a benzoic acid derivative from the leaves of Cyclocarya paliurus and their glucosidase and glycogen phosphorylase inhibiting activities

Two novel compounds, 3-methoxypterolactone ( 1) and 2-amino-3,4-dihydroxy-5-methoxybenzoic acid ( 2), were isolated from leaves of Cyclocarya paliurus (Batal.) Iljinsk, together with nine known compounds: pterolactone ( 3), gallic acid ( 4), 4-hydroxy-3-methoxybenzoic acid ( 5), oleanolic acid ( 6), beta-boswellic acid ( 7), alpha-boswellic acid ( 8), beta-amyrin ( 9), beta-amyrone ( 10) and 3beta-O-trans-caffeoyl-morolic acid ( 11). The structure elucidation was based on spectroscopic methods, including two-dimensional NMR experiments ( (1)H- (1)H COSY, HMQC and HMBC). All isolated compounds were evaluated for their glycosidase and glycogen phosphorylase inhibitory activities. 2-Amino-3,4-dihydroxy-5-methoxybenzoic acid and gallic acid showed significant alpha-glucosidase and glycogen phosphorylase inhibitory activities.     

中药升麻酚酸类化学成分研究

目的寻找中药升麻(Cimicifuga foetida L.)的有效成分.方法利用硅胶柱及Sephadex LH-20柱进行分离纯化,根据理化性质和各种波谱数据(UV,IR,MS,1D及2D-NMR),辅以化学方法,进行结构鉴定.结果从升麻生药乙醇提取物分离的酚酸性部分获得了8种成分:升麻酸(cimicifugic acid,1),马栗树皮素(esculetin,2),咖啡酸甲酯(caffeic acid methyl ester,3),4-氧-乙酰基咖啡酸(4-O-acetyl-caffeic acid,4),芥子酸(sinapic acid,5),咖啡酸(caffeic acid,6),阿魏酸(ferulic acid,7),异阿魏酸(isoferulic acid,8).结论 1为新的化合物,命名为升麻酸;2～7为首次从该生药中分得.     

杏香兔耳风化学成分的分离与鉴定

目的研究杏香兔耳风(Ainsliaea fragransChamp.)的化学成分,为活性测试及制定质量标准提供依据。方法利用多种色谱方法进行分离纯化,并根据理化性质和波谱数据确定化合物结构。结果分离并鉴定了9个化合物,其中1个倍半萜:(-)-clovane-2β,9α-diol(1);2个三萜:降香萜醇乙酸酯(bauerenyl acetate,2)、熊果酸(ursolic acid,3);1个寡肽:橙黄胡椒酰胺乙酸酯(aurantiamideacetate,4);5个有机酸类化合物:丁香酸(syringic acid,5)、香草酸(vanillic acid,6)、琥珀酸(succin-ic acid,7)、辛二酸(octanedioic acid,8)、二十六酸(hexacosanic acid,9)。结论上述化合物均为首次从兔儿风属植物中分离得到。     

飞龙掌血的三萜类成分研究

目的：研究飞龙掌血茎的乙酸乙酯部位的化学成分。方法：采用硅胶柱色谱、sephadex LH-20凝胶柱色谱、MCI以及半制备高效液相色谱等多种分离手段进行分离纯化,并结合化合物的理化性质及核磁数据进行结构鉴定。结果：从飞龙掌血茎的乙酸乙酯部位中分离并鉴定出8个三萜类化合物,分别为：2α, 3β, 19α-三羟基-12-烯-28-乌苏酸（1）,1β-羟基蔷薇酸（2）, 2α, 3α, 19α, 23-四羟基-12-烯-28-乌苏酸（3）, 2α, 3α, 19α-trihydroxyurs-12-en-23-formyl-28-oic acid（4）,乌苏酸（5）, 2α-羟基乌苏酸（6）, 山楂酸（7）,oleanolic acid（8）。结论：化合物1~8均为首次从该属中分离得到。     

Alkamides from Cissampelos glaberrima

Two alkamides, deca-2E,4E-dienoic acid isobutylamide (1) and octa-2E,4E-dienoic acid isobutylamide (2), were isolated from the roots of Cissampelos glaberrima (Menispermaceae). Their structures were established by spectral and physical methods. Traces of two new alkamides, decen-2-oic acid isobutylamide (3) and decanoic acid isobutylamide (4), were identified by mass spectrometry.     

爪哇脚骨脆中三萜类化学成分研究(英文)

从大风子科植物爪哇脚骨脆的树干中分离得到11个已知三萜类化合物,经波谱解析及与文献对照方法,分别鉴定为木栓酮-2,3-内酯(1),木栓烷(2),表木栓醇(3),木栓酮(4),2α,3α,19α-三羟基-乌苏烷-12-烯-28-羧酸(5),2α,3β,19α-三羟基-乌苏烷-12-烯-28-羧酸(6),2α,3α,23-三羟基-乌苏烷-12-烯-28-羧酸(7),2α,3α,23-三羟基-齐墩果烷-12-烯-28-羧酸(8),2α,3α,19α,23-四羟基-乌苏烷-12-烯-28-羧酸(9),2α,3α,19α,23-四羟基-乌苏烷-12-烯-28-羧酸-28-O-β-D-葡萄糖酯(10),及3β,19α-双羟基-乌苏烷-12-烯-28-羧酸3-O-α-L-阿拉伯糖苷(11).其中化合物1为大风子科植物中稀有的裂环木栓烷三萜,所有化合物皆为首次从本植物中分离得到。     

野西瓜果皮中有机酸类化学成分的分离与鉴定

目的研究野西瓜(Capparis spinosa L.)果皮中有机酸类化学成分,为进一步阐明其抗炎抗菌活性成分、充分开发利用提供科学依据。方法采用硅胶柱色谱、羟丙基葡聚糖凝胶柱色谱等方法进行分离纯化,并通过理化常数测定和波谱分析鉴定其化学结构。结果从野西瓜果皮中分离得到了6个有机酸类化合物,即香草酸(vanillic acid,1)、4-羟基苯甲酸(4-hydroxybenzoic acid,2)、原儿茶酸(protocatechuic acid,3)、2,4-二羟基苯甲酸(2,4-dihydroxybenzoic acid,4)、丁二酸(succinic acid,5)、邻苯二甲酸二(2-乙基)己酯(bis(2-ethylhexyl)phthalate,6);分离得到3个甾体类化合物为β-谷甾醇(β-sitosterol,7)、胡萝卜苷(daucosterol,8)、α-菠菜甾醇-3-O-β-D-吡喃葡萄糖苷(α-spinasterol-3-O-β-D-glucopyranoside,9)。结论化合物4、6、9为首次从山柑属植物中分离得到。     

怀菊花茎叶中一个新聚炔类化合物

采用多种色谱技术从怀菊花茎叶中分离得到8个聚炔类化合物。通过UV、IR、ESI-MS、HR-ESI-MS及NMR等波谱技术对其进行结构鉴定,分别为菊花新炔C (2E,4E,12Z-十四碳三烯-1-吡咯烷基-1-酮-8,10-二炔)(1)、tetradeca-2E, 4E, 12E-trien-8, 10-diynoic acid pyrrolidide (2)、tetradeca-2E, 4E-dien-8, 10-diynoic acid pyrrolidide (3)、tetradeca-2E,4E,10Z-trien-8-ynoic acid pyrrolidide (4)、2E,4E,12E-tetradecatriene-8,10-diynoic acid isobutylamide (5)、2E,4E-undecyldiene-8,10-diynoic acid isobutylamide (6)、2E,4E,10E-N-isobutyl-2,4,10-tetradecatrien-8-ynoic acid amide(7)和十一-2E,4E-二烯-8,10-二炔酸苯乙胺(...     

Six new triterpenoid saponins from the root and stem bark of Cephalanthus occidentalis

From the root and stem bark of Cephalanthus occidentalis L., a common American wetland species, six new triterpenoid saponins (1 - 6), together with 29 known compounds were isolated and identified. On the basis of spectroscopic analysis and chemical degradation, the structures of new compounds were elucidated as 3-O-beta-glucopyranosylcincholic acid (1), cincholic acid 28-O-beta-glucopyranosyl ester (2), 3-O-beta-glucopyranosyl-(1-->4)-beta-fucopyranosylcincholic acid (3), 3-O-beta-glucopyranosyl-(1-->4)-beta-fucopyranosylcincholic acid 28-O-beta-glucopyranosyl ester (4), 3-O-beta-glucopyranosylcincholic acid 28-O-alpha-arabinopyranosyl-(1-->2)-beta-glucopyranosyl ester (5), and 3-O-beta-glucopyranosylquinovic acid 28-O-alpha-arabinopyranosyl-(1-->2)-beta-glucopyranosyl ester (6).     

叶枯灵在大鼠尿内的代谢产物分析

大鼠ig叶枯灵后,用HPLC和TLC分离纯化尿提取液中的叶枯灵及其代谢产物,得到六个组分,经MS和UV初步鉴定为叶枯灵,苯甲酸,马尿酸和三个新化合物:肼叉-1.3-二噻茂烷,乙酰肼叉-1.3-二噻茂烷和丙酮酸缩肼叉-1.3-二噻茂烷,通过对代谢物的化学合成,进一步确证了它们的化学结构。     

土槿皮的化学成分研究(英文)

对松科Pinaceae植物金钱松Pseudolarix kaempferi Gord的干燥根皮—土槿皮的化学成分进行了研究。应用硅胶,Sephadex LH-20,MCI柱色谱以及高效液相色谱等技术进行分离纯化,利用化合物的理化常数和波谱数据鉴定其结构。分离并鉴定了26个化合物,分别为:土槿丁酸(1),土槿甲酸(2),土槿乙酸(3),土槿丙酸(4),3β-乙酰氧基-齐墩果烷-11,13(18)二烯(5),3β-乙酰氧基-齐墩果烷-9(11),12二烯(6),3β-羟基-齐墩果烷-11,13(18)二烯(7),3β-羟基-齐墩果烷-9(11),12二烯(8),Celangulatin C(9),Celangulatin E(10),17β-通关藤苷元B(11),11α-O-(2-甲基丁酰基)-12β-O-乙酰基通关藤苷元B(12),11α-O-(2-甲基丁酰基)-12β-O-巴豆酰基(顺芷酰基)通关藤苷元B(13),β-谷甾醇酯(14),伞形花内酯(15),5,7-二羟基香豆素(16),花椒毒素(17),异虎耳草素(18),芒柄花黄素(19),毛蕊异黄酮(20),Cnidimol B(21),胸腺嘧啶(22),3-呋喃甲酸(23),2-呋喃甲酸(24),香草酸(25),原儿茶酸(26)。其中化合物5–24为首次从该植物中分离得到;并首次归属化合物1的1H和13CNMR谱数据。     

牻牛儿苗化学成分研究

目的对牻牛儿苗的化学成分进行研究。方法利用溶剂提取和凝胶柱层析进行分离,根据化合物的理化性质和光谱数据鉴定其结构。结果得到8个化合物,分别确定为:没食子酸(1),3-O-galloyl-(-)-shikimic acid(2),3,4-di-O-galloyl-(-)-shikimic acid(3),3,5-di-O-galloyl-(-)-shikimic acid(4),β-谷甾醇(5),山柰酚(6),槲皮素(7)和鞣花酸(8)。结论化合物2～8都是首次从该植物中分离得到。     

单麻叶千里光中的一个新化学成分

研究菊科千里光属植物单麻叶千里光Senecio cannabifolius var integrilifolius (Koidz.) Kitam.的化学成分。用硅胶、 Sephadex LH-20及反相C₁₈等柱色谱方法从其水提取物中分离得到11个化合物， 根据理化性质和光谱数据鉴定其结构， 分别为senecine(1)， 对羟基苯乙酸(2)， 原儿茶酸(3)， 2,5-二羟基苯乙酸(4)， 3,4-二羟基苯乙酸(5)， 香草酸(6)， 咖啡酸(7)， 丁二酸(8)， 2-糠酸(9)， 1,2,4,5-四氢-jacaranone(10)， 4-(吡咯烷-2-酮基)-苯基乙酸(11)。其中化合物1为新化合物， 2～11均为首次从该植物中分离得到。     

Inhibitory Constituents against Cyclooxygenases fromAralia cordata Thunb

Seven diterpenes, four polyacetylenes, a lipid glycerol, and two sterols were isolated from the methylene chloride fraction of the root of Aralia cordata. Their chemical structures were determined as (-)-pimara-8(14),15-dien-19-oic acid (2), pimaric acid (3), (-)-kaur-16-en-19-oic acid (4), 17-hydroxy-ent-kaur-15-en-19-oic acid (9), 7alpha-hydroxy-(-)-pimara-8(14),15-dien-19-oic acid (10), 16alpha,17-dihydroxy-(-)-kauran-19-oic acid (11), 16-hydroxy-17-isovaleroyloxy-ent-kauran-19-oic acid (12), falcarindiol (5), dehydrofalcarindiol (6), dehydrofalcarindiol-8-acetate (7), falcarindiol-8-acetate (8), alpha-mono palmitin (13), stigmasterol (1), and daucosterol (14) by the spectral evidences. These compounds were tested with COX-1 and COX-2 inhibition assays. This study found that compounds 2, 4, 5, 6, 7, 8, and 10 inhibited COX-1 dependent conversion of the exogenous arachidonic acid to PGE2 in a dose-dependent manner with IC50 values of 134.2 microM, 121.6 microM, 170 microM, 50.4 microM, 11.7 microM, 99.6 microM, and 69.6 microM, respectively. But, most of these compounds weakly inhibited COX-2 dependent PGE2 generation. Among them, only compound 4 showed relatively significant inhibitory activity (IC50: 127.6 microM).     

卷柏中化学成分的分离与结构鉴定

目的研究中药卷柏(Selaginella tamariscina (Beauv.) Spring)水提物中的化学成分。方法用Sephadex LH-20及硅胶等色谱技术进行分离纯化,根据理化性质、光谱数据进行结构鉴定。结果得到9个化合物,分别鉴定为(2r,3s)-二氢-2-(3′,5′-二甲氧基-4′-羟基苯基)-3-羟甲基-7-甲氧基-5-乙酰基苯骈呋喃(1),3-羟基-苯丙酸-(2′-甲氧基-4′-羧基苯酚)酯(卷柏酯A,2),丁香脂素(3),1-(4′-羟基-3′-甲氧基苯基)丙三醇(4),阿魏酸(5),咖啡酸(6),香荚兰酸(7),丁香酸(8),7-羟基香豆素(9)。结论化合物1,2为新化合物,其余化合物均为首次从卷柏属植物中分离得到。     

胶陀螺中有机酸类化学成分

目的研究胶陀螺(Bulgaria inquinans Fr.)中有机酸类化学成分。方法采用溶剂萃取法、反复硅胶柱色谱、重结晶等手段分离胶陀螺中的化学成分,通过理化常数测定和光谱分析鉴定其化学结构。结果从胶陀螺体积分数为70%的乙醇溶液提取物乙酸乙酯萃取层及正丁醇萃取层中分离得到了10个有机酸类化合物,即草酸(oxalic acid,1)、丁二酸(succinic acid,2)、棕榈酸(palmitic acid,3)、对羟基苯甲酸(4-hydroxybenzoic acid,4)、2,4-二羟基苯甲酸(2,4-dihydroxybenzoic acid,5)、异香草酸(isovanillic acid,6)、咖啡酸(caffeic acid,7)、香豆酸(courmaric acid,8)、原儿茶酸(protocatechuic acid,9)、肉桂酸(cinnamic acid,10)。结论化合物2-10均为首次从该属植物中分离得到。     

Three new phenolic compounds from the lichen Thamnolia vermicularis and their antiproliferative effects in prostate cancer cells

Three new phenolic compounds, thamnoliadepsides A (1), B (2), and thamnolic acid A (3), and seven known compounds, everninic acid (4), baeomycesic acid (5), β-orcinol (6), β-resorcylic acid (7), ethyl orsellinate (8), squamatic acid (9), and vermicularin (10), were isolated from the lichen Thamnolia vermicularis (Sw.) Ach. ex Schaerer. Their structures were determined based on spectroscopic analysis, including 2D-NMR experiments and HR-MS techniques. Compound 1 inhibited growth of prostate cancer cells and bonded to G-quadruplex DNA based on NMR determination.