MMP-3、MMP-9、TIMP-1在子宫腺肌病中的表达及其意义

 目的 研究MMP-3、MMP-9、TIMP-1在子宫腺肌病中的表达,探讨子宫腺肌病的发病机制.方法 采用二步法免疫组化的方法检测子宫肌腺病45例在位内膜(A组)、36例异位内膜(B组)和28例对照组内膜中的MMP-3、MMP-9、TIMP-1含量.结果 MMP- 9在研究组A、研究组B中的表达强于对照组,差异有统计学意义(P<0.05);MMP-3在研究组A、研究组B中的表达强于对照组,差异有统计学意义(P<0.05);TIMP-1在对照组中的阳性表达弱于研究组A,差异有统计学意义(P<0.05),研究组A强于研究组B的阳性表达,差异有统计学意义(P<0.05).结论 子宫腺肌病的发病可能与MMP-3、MMP-9、TIMP-1的异常表达有关.     

秋水仙碱对人瘢痕疙瘩成纤维细胞MMP-1､MMP-2表达的影响

【摘要】 目的 研究探讨秋水仙碱对体外培养人瘢痕疙瘩成纤维细胞(keloid fibroblast, KFB) 内基质金属蛋白酶-1(matrix metalloproteinase-1, MMP-1)基质金属蛋白酶-2 (MMP-2) 和基质金属蛋白酶-9 (MMP-9) 表达的影响?方法 取7 例患者的瘢痕疙瘩组织进行体外成纤维细胞培养, 待细胞培养至第3 代时, 加入浓度为0.125%的胰蛋白酶消化液浸泡至细胞质回缩?细胞间隙增大后, 转种于75.0 cm2 的培养瓶中继续培养; 待细胞基本融合成片(密度达85%时) 后, 分别将每例患者瘢痕组织的传代培养细胞分为A 组?B 组?C 组?D 组及E组, A 组加入含胎牛血清的DMEM 培养基继续培养备用, B 组?C 组?D 组?E 组加入含胎牛血清的DMEM 培养基及浓度分别为2?4?8?16 μg/ mL 的秋水仙碱继续培养24 h (37℃, 5.0% CO2 ) 后备用?采用逆转录聚合酶链反应(real-time PCR, RT-PPCR) 法检测基质金属蛋白酶mRNA 的表达情况, Western Blot 法检测基质金属蛋白酶蛋白的表达情况?结果 A 组?B 组?C 组?D 组?E 组KFB 中MMP-1 mRNA 的表达水平分别为(0.174±0.016)?(0.282±0.015)?(0.369±0.022)?(0.541±0.027) 及(0.672±0.020), MMP-2 mRNA 的表达水平分别为(0.201±0.033)?(0.371±0.026)?(0.548±0.031)?(0.653 ±0.019) 及(0.796±0.024), MMP-1 蛋白的表达水平分别为(0.127±0.031)?(0.205±0.013)?(0.293±0.027)?(0.349±0.034) 及(0.512±0.035), MMP-2 蛋白的表达水平分别为(0.278±0.023)?(0.395±0.036)?(0.526±0.025)?(0.673±0.014)及(0.792±0.037), 而MMP-9 mRNA 及MMP-9 蛋白均未见表达?与A 组对比, B 组?C 组?D 组及E 组KFB中MMP-1 mRNA?MMP-2mRNA?MMP-1 蛋白及MMP-2 蛋白的表达水平均较高, P均< 0.01, 差异具有统计学意义, 且随着秋水仙碱浓度的增高, B 组?C 组?D 组及E 组KFB 中MMP-1 mRNA?MMP-2 mRNA?MMP-1蛋白及MMP-2 蛋白的表达水平均呈逐渐增高的趋势, P均< 0.01, 差异具有统计学意义?结论 秋水仙碱可提高KFB 中MMP-1 与MMP-2 的表达水平, 从而达到抗纤维化?抑制瘢痕疙瘩形成的目的, 且在一定浓度范围内, 其作用随着秋水仙碱浓度的增加而逐渐增强, 有望成为临床治疗瘢痕疙瘩的一线药物?     

MEBO对糖尿病溃疡大鼠创面组织中MMP-9、MMP-2、TIMP-1、TIMP-2动态表达的影响

目的 探讨分析湿润烧伤膏（MEBO）对糖尿病溃疡大鼠创面组织中基质金属蛋白酶-9（MMP-9）、基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶抑制剂-1（TIMP-1）及基质金属蛋白酶抑制剂-2（TIMP-2）表达水平的影响。方法 从160只健康雄性SD大鼠中随机选取120只制备大鼠糖尿病模型,其余40只正常喂养;最终选取90只造模成功大鼠随机分为模型组、MEBO组与bFGF组,每组30只,与此同时随机选取未予特殊处理的30只大鼠作为空白组,所有大鼠均做全层皮肤缺损创面,空白组与模型组大鼠创面予以凡士林油纱换药处理、MEBO组大鼠创面予以MEBO药纱换药处理、bFGF组大鼠创面予以重组牛碱性成纤维细胞生长因子（rb-bFGF）药纱换药处理,对比观察各组大鼠干预第4、6、12天时创面愈合率以及MMP-9、MMP-2、TIMP-1、TIMP-2与胶原蛋白水平变化情况。结果 （1）干预第4、6、12天,模型组大鼠创面愈合率均明显低于其他各组（P均＜0.05）,空白组大鼠创面愈合率均明显高于其他各组（P均＜0.05）,而MEBO组与bFGF组间无明显差异（P均＞0.05）。（2）干预第4、6、12天,模型组大鼠创面组织中MMP-9及MMP-2 mRNA表达水平均明显高于其他各组（P均＜0.05）,TIMP-1及TIMP-2 mRNA表达水平均明显低于其他各组（P均＜0.05）;除干预第6天MEBO组大鼠创面组织中TIMP-1 mRNA表达水平明显高于bFGF组（P＜0.05）外,其余各时间点MEBO组与bFGF组大鼠创面组织中MMP-9、MMP-2、TIMP-1及TIMP-2 mRNA表达水平均无明显差异（P均＞0.05）。（3）干预第4、6、12天,模型组大鼠创面组织中胶原蛋白水平明显低于其他各组（P均＜0.05）;除干预第4天MEBO组大鼠创面组织中胶原蛋白水平明显高于bFGF组（P＜0.05）外,其余各时间点MEBO组与bFGF组大鼠创面组织中胶原蛋白水平均无明显差异（P均＞0.05）。结论 MEBO可通过调控糖尿病溃疡大鼠创面组织中MMP-9、MMP-2、TIMP-1、TIMP-2的表达水平改善胶原蛋白过度降解,维持细胞外基质代谢平衡,进而促进创面愈合,调节MMP-9/TIMP-1、MMP-2/TIMP-2的比例失衡可能是其促进糖尿病溃疡创面愈合的作用靶点。     

Problems in determining A2B group specific properties in blood stains

Summary Blood stains belonging to various sub-group A combinations were examined by the absorption-elution method (in comparison with Holzer method and mixed agglutination). No difficulties were encountered in the identification of A₁, A₂, and A₁B blood stains, while identification of A antigen in A₂B stains was dubious (always negative with the others methods). This seemed to be due to low anti-sera titer in relation to A antigen in this subgroup combination.

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Zusammenfassung Mit Hilfe der Absorptions-Elutionsmethode wurden Blutspuren der verschiedenen A-Untergruppenkombination untersucht. Keine Schwierigkeiten traten bei Blutspuren, die A₁, A₂ und A₁B enthielten, auf. Dagegen stieß man auf Schwierigkeiten bei der A-Antigenidentifikation in A₂B-Blutspuren. Wahrscheinlich ist dies auf den niedrigen Titer der Antisera dem A-Antigen dieser Untergruppenkombination gegenüber zurückzuführen.

     

湿润烧伤膏对糖尿病足创面组织中MMP-2、MMP-9、Bcl-2、Bax水平的影响

【摘要】目的探讨湿润烧伤膏(MEBO)对糖尿病足创面组织中基质金属蛋白酶(MMP)-2、MMP-9、B细胞淋巴瘤-2(Bcl-2)、Bcl-2相关X蛋白(Bax)水平的影响。方法选取2020年5月至2022年5月郑州大学附属郑州中心医院收治的55例糖尿病足患者作为研究对象,按照不同治疗方法将其分为MEBO组(35例)和VSD组(20例),MEBO组患者局部创面采用MEBO治疗,VSD组患者局部创面采用负压封闭引流(VSD)治疗,对比观察两组患者创面面积,创面组织中MMP-2、MMP-9、Bcl-2、Bax水平及临床疗效。结果治疗第7、21天,MEBO组患者创面面积均明显小于VSD组(t=2.719、5.268,P=0.009、P<0.001),创面组织中MMP-2、MMP-9、Bax水平均明显低于VSD组(MMP-2:t=2.138、2.202,P=0.037、0.032;MMP-9:t=2.129、2.476,P=0.038、0.017;Bax:t=3.623、3.038,P=0.001、0.004),Bcl-2水平均明显高于VSD组(t=2.040、3.054,P=0.046、0.004);治疗21d后,MEBO组患者中显效23例、有效10例、无效2例,明显优于VSD组患者的显效8例、有效7例、无效5例(Z=-2.126,P=0.033)。结论MEBO可通过降低糖尿病足创面组织中MMP-2、MMP-9、Bax水平以及提高Bcl-2水平促进创面愈合。     

MMP-1、MMP-13在大鼠骨关节炎软骨中的表达

目的观察MMP-1和MMP-13在大鼠前交叉韧带切断加内侧半月板切除的骨关节炎模型中的表达,探讨MMP-1和MMP-13表达与骨关节炎（OA）进程的关系。方法将25只SD大鼠右膝行前交叉韧带切断加内侧半月板切除术（ACLT＋MMx）,对侧作为空白对照,分别于术后1周、2周、4周、6周、10周处死,每次随机处死5只,另取5只SD大鼠仅行右膝关节切开术作为假手术组,于术后10周处死,对膝关节软骨标本进行大体和组织学切片观察及评分,用ELISA法检测MMP-1、MMP-13在关节软骨中的表达。结果 ACLT＋MMx组Mankin评分由1周的1.0分到4周的6.2分发展到10周的13.6分,与假手术组及空白对照组相比有显著差异（P〈0.01）。ACLT＋MMx组MMP-13的表达在OA早期增高,中重度期后出现明显下降趋势（P〈0.01）;MMP-1的表达则随着病程持续升高,与病变严重程度呈正相关性（P〈0.01）。假手术组及空白对照组软骨细胞几乎不表达MMP-1及MMP-13。结论 MMPs在OA的发病机制中有重要作用,MMPs各亚型在OA进程中的表达各不相同。     

MMP-2, jumping exercise and nandrolone in skeletal muscle

Matrix metalloproteinases (MMPs) are crucial to the development and maintenance of healthy tissue. The aim of this study was to investigate MMP-2 activity in gastrocnemius, soleus, extensor digitorium longus (EDL) and tibialis anterior (TA) muscles after exercise associated with an anabolic androgenic steroid (AAS). Wistar rats were grouped into: sedentary (S); sedentary with AAS (AAS); trained without (T) and with (AAST) AAS. Exercised groups performed a 7-week water-jumping program. The exercise increased MMP-2 activity in gastrocnemius and soleus, but not in TA and EDL. The AAS treatment decreased MMP-2 activity of EDL, as compared to the S group, with no effect in gastrocnemius and soleus. The association of AAS and training restored the normal MMP activity of EDL. Training increases tissue turnover in primary motor muscles, which in turn respond differently to AAS treatment. This data may be useful for a better understanding of muscular physiology under AAS use.     

NMR properties of human median nerve at 3 T: Proton density,T1, T2, and magnetization transfer

Purpose

To measure the proton density (PD), the T1 and T2 relaxation time, and magnetization transfer (MT) effects in human median nerve at 3 T and to compare them with the corresponding values in muscle.

Materials and Methods

Measurements of the T1 and T2 relaxation time were performed with an inversion recovery and a Carr‐Purcell‐Meiboom‐Gill (CPMG) imaging sequence, respectively. The MT ratio was measured by acquiring two sets of 3D spoiled gradient‐echo images, with and without a Gaussian saturation pulse.

Results

The median nerve T1 was 1410 ± 70 msec. The T2 decay consisted of two components, with average T2 values of 26 ± 2 msec and 96 ± 3 msec and normalized amplitudes of 78 ± 4% and 22 ± 4%, respectively. The dominant component is likely to reflect myelin water and connective tissue, and the less abundant component originates possibly from intra‐axonal water protons. The value of proton density of MRI‐visible protons in median nerve was 81 ± 3% that of muscle. The MT ratio in median nerve (40.3 ± 2.0%) was smaller than in muscle (45.4 ± 0.5%).

Conclusion

MRI‐relevant properties, such as PD, T1 and T2 relaxation time, and MT ratio were measured in human median nerve at 3 T and were in many respects similar to those of muscle. J. Magn. Reson. Imaging 2009;29:982–986. © 2009 Wiley‐Liss, Inc.     

利用特异性识别N蛋白抗体建立SARS-CoV-2的快速检测方法

目的 筛选特异性识别严重急性呼吸综合征冠状病毒2(SARS-CoV-2)核衣壳蛋白(N)高亲和力抗体,并建立一种基于双抗夹心酶联免疫吸附测定(ELISA)快速检测SARS-CoV-2 N蛋白的方法.方法 使用噬菌体展示技术筛选特异性识别SARS-CoV-2 N蛋白的抗体,用ELISA和生物膜干涉技术(BLI)验证抗体结...     

Defining screening panel of functional variants of CYP1A1, CYP2C9, CYP2C19, CYP2D6, and CYP3A4 genes in Serbian population

International Journal of Legal Medicine - Plethora of drugs and toxic substances is metabolized by cytochrome P450 enzymes (CYP450). These enzymes are coded by highly variable genes abundant with...     

抗细胞角蛋白单克隆抗体AE_1/AE_3在食管、贲门、胃和大肠癌组织中的反应

应用抗细胞角蛋白单克隆抗体ＡＥ１／ＡＥ３，对食管癌、胃癌和大肠癌的原发组织反应进行了研究。结果表明，４６例癌组织的反应率是９３％，食管癌为１００％，胃癌和大肠癌分别是９４％和７９％；反应强度和反应范围显示，食管癌较胃和大肠癌好。ＡＥ１／ＡＥ３符合做检测食管癌在非上皮组织（淋巴结、骨髓和外周血）中的微小转移的探针，不宜用来检测胃和大肠癌的微小转移，至少不能单独使用。同为胃和大肠来源的腺癌组织可出现阴性反应，说明组织来源完全相同的肿瘤可有不同的ＣＫ亚型。对抗某一个或几个ＣＫ亚型单克隆抗体阴性反应的肿瘤，不能完全除外为上皮来源。鉴别疑难肿瘤的组织来源时，必须应用多个抗ＣＫ的单克隆抗体，并且结合其他标记才更有临床意义。     

Immunohistochemical detection of MMP-2 and MMP-9 in a stasis-induced deep vein thrombosis model and its application to thrombus age estimation

We immunohistochemically examined the expression of matrix metalloproteinase (MMP)-2 and MMP-9 using venous thrombi developed by ligation of the inferior vena cava (IVC) in mice. Both MMP-2- and MMP-9- positive cells could be detected in the whole course of thrombus formation after IVC ligation. Morphometrically, their number was greatest 14 days after IVC ligation and thereafter, gradually decreased at 21 days. The number of MMP-9-positive cells was significantly higher than that of MMP-2-positive cells at 1 to 7 days. The average ratio of MMP-9 to MMP-2 (MMP-9/MMP-2 ratio) was >2.0 in all thrombus samples at 1–5 days. After 7 days, the MMP-9/MMP-2 ratio was less than 2.0. These observations implied that an MMP-9/MMP-2 ratio markedly exceeding 2.0 strongly indicates an age of 5 days or less. Furthermore, an MMP-9/MMP-2 ratio of <2.0 probably indicates an age of more than 7 days. The present study demonstrated that the immunohistochemical detection of intrathrombotic MMP-2 and MMP-9 was suitable to estimate the age of venous thrombi.     

抗细胞角蛋白单克隆抗体AE1／AE3在肿瘤诊断中的初步探讨

应用抗细胞角蛋白单克隆抗体ＡＥ１／ＡＥ３，对部分非上皮细胞和细胞，以及上皮来源的恶性肿瘤笤乳腺癌、肺癌和鼻咽吕组织和细胞的角蛋白表达进行了研究。结果表明，ＡＥ１／ＡＥ３对骨髓、外周血、淋巴结和淋巴瘤的组织和细胞基本无交叉反应，而对上述癌组织和细胞则有不同程度的反应。     

Labelling of monoclonal antibodies 10B, 8C,and M2A with Indium-111

Monoclonal antibodies 10B, 8C and M2A were conjugated with cyclic DTPA anhydride to a substitution level of 1–2 mol DTPA/mol antibody. The DTPA-conjugated antibodies were purified by size-exclusion mini-column chromatography and labelled with [¹¹¹In]In acetate to a specific activity of 37–74 kBq/μg (1–2 μCi/μg). The radiochemical purity of the antibody preparation was 76–81%. The immunoreactive fraction of the radiolabelled antibodies ranged from 0.17 to 0.31, significantly lower than that of their radioiodinated analogs.     

Immunohistochemical examination of skin wounds with antibodies against alpha-1-antichymotrypsin,alpha-2-macroglobulin and lysozyme

The distribution of the proteinase inhibitors alpha-1-antichymotrypsin (α1-act), alpha-2-macroglobulin ((α-2-m) and lysozyme was analysed immunohistochemically in 27 intravitally acquired wounds, 3 postmortem skin lacerations and 9 specimens of undamaged skin. Intravitally acquired wounds demonstrated distinct positive reactions for all antibodies examined (α-1-act 66.6%; α-2-m 51.9%; lysozyme 25.9%). However the undamaged skin margins opposite the wound margins also gave positive reactions (a-l-act 51.8%; (α-2-m 37.0%; lysozyme 25.9%). Nearly half of the control cases (specimens of undamaged skin) exhibited weak positive reactions for all 3 antibodies. These could be easily distinguished from the strong positive reactions observed in intravitally acquired wounds. False positive reactions were observed due to contamination resulting from contact with serum components, in cases of advanced autolysis of specimens, and as a result of fixation and drying artefacts. Even though immunohistochemical studies of α-1-act, (α-2-m and lysozyme give some indications concerning wound vitality, they cannot be considered as proof because irrefutable differentiation of true positive and false positive reactions is not possible in all cases.     

MMPs/TIMPs系统在小鼠急性放射性肺损伤中的表达

目的 通过检测基质金属蛋白酶（MMPs）和基质金属蛋白酶组织抑制物（TIMPs）在单次大剂量胸部照射小鼠肺组织中的表达,探讨全肺照射诱导的MMPs/TIMPs系统表达紊乱在放射性肺损伤发生与发展中的作用。方法 实验组小鼠在出生后8周时给予单次胸部照射12 Gy,对照组小鼠给予假照射。在照射后4和8周时处死小鼠,收集肺组织标本,测定肺组织中MMP-2、MMP-9、MMP-3、MMP-13、TIMP-1、TIMP-2和TIMP-3蛋白的表达。结果 照射后4和8周时,实验组小鼠肺组织中MMP-2表达水平分别为对照组的1.7和1.9倍,MMP-9表达水平分别为对照组的2.7和2.6倍,而MMP-3、MMP-13、TIMP-1、TIMP-2、和TIMP-3的表达水平与对照组比较无显著改变。结论 辐射诱导小鼠肺组织中MMP-2和MMP-9的表达增加,可能加剧了急性放射性肺损伤。     

冬凌草甲素抑制BGC823细胞的生长及MMP-2 MMP-9的表达

目的探讨冬凌草甲素（oridonin,Ori）在体内、外对胃癌BGC823细胞生长的影响,及对BGC823移植瘤MMP-2、MMP-9表达的影响。方法MTT法检测Ori对BGC823细胞生长的抑制作用,流式细胞术检测其对BGC823细胞周期的影响,体内抑瘤实验观察其对BGC823移植瘤生长的影响,免疫组织化学染色法观察Ori对移植瘤组织中MMP-2、MMP-9表达的影响。结果Ori在体外显著抑制细胞的增殖,引起细胞G2/M期阻滞;体内实验表明,Ori显著抑制肿瘤组织的生长,抑制率达70.7%;免疫组化染色结果显示,Ori显著抑制移植瘤MMP-2及MMP-9的表达。结论Ori在体内、外显著抑制胃癌BGC823细胞的生长,减少移植瘤MMP-2、MMP-9的表达,提示Ori可能具有抑制肿瘤侵袭转移及血管生成的作用。     

Frequenzen von Gm 1, 2, 3, 5, 10, 21 und Km 1, 3 in Portugal und Schleswig-Holstein

Zusammenfassung Es wurden 1000 Schleswig-Holsteiner und erstmalig 562 Portugiesen auf die Gm-Faktoren 1, 2, 3, 5, 10 und 21 sowie die Km-Faktoren 1 und 3 untersucht und die Phänotypen mit ihren Genfrequenzen für diese Kollektive bestimmt. Die Frequenzergebnisse wurden miteinander verglichen und Unterschiede herausgestellt.Ausführliche Darstellung siehe Dissertation Zippel, Lübeck (1981)