胰岛素治疗对血清中胰岛素样生长因子I水平的影响

探讨胰岛素治疗对2型糖尿病患者血清中胰岛素样生长因子I(IGF-I)水平的影响以及两者之间的关系.结果 发现2型糖尿病患者外源性胰岛素治疗可增加血清中的IGF-I水平[(126.70±51.91对90.04±43.68)μg/L,P<0.01],并且IGF-I水平与胰岛素水平呈正相关(r=0.298,P<0.05).

Abstract：

To explore the effect of insulin therapy on serum level of insulin-like growth factor-I(IGF-I)in patients with type 2 diabetes mellitus.The results showed that serum IGF-I level increased[(126.70±51.91 vs 90.04±43.68)μg/L,P<0.01]and was positively correlated with insulin level in patients with type 2 diabetes mellitus after exogenous insulin therapy(r=0.298,P<0.05).     

胰岛素治疗对血清中胰岛素样生长因子I水平的影响

To explore the effect of insulin therapy on serum level of insulin-like growth factor-I(IGF-I)in patients with type 2 diabetes mellitus.The results showed that serum IGF-I level increased[(126.70±51.91 vs 90.04±43.68)μg/L,P<0.01]and was positively correlated with insulin level in patients with type 2 diabetes mellitus after exogenous insulin therapy(r=0.298,P<0.05).     

胰岛素治疗对血清中胰岛素样生长因子I水平的影响

To explore the effect of insulin therapy on serum level of insulin-like growth factor-I(IGF-I)in patients with type 2 diabetes mellitus.The results showed that serum IGF-I level increased[(126.70±51.91 vs 90.04±43.68)μg/L,P<0.01]and was positively correlated with insulin level in patients with type 2 diabetes mellitus after exogenous insulin therapy(r=0.298,P<0.05).     

胰岛素样生长因子在肾透明细胞癌患者血液中表达及其临床意义

目的 探讨胰岛素样生长因子(IGF)Ⅰ、Ⅱ和胰岛素样生长因子结合蛋白(IGFBP)3、5在肾透明细胞癌患者外周血液中的表达及其临床意义.方法 选择2007年5月至2009年12月在我院手术治疗的肾透明细胞癌患者40例(肾癌组),对照组为同期的肾积水患者16例,采用酶联免疫吸附法(ELISA)检测两组患者血清标本中IGF-Ⅰ、Ⅱ和IGFBP3、5的水平.结果 肾癌组术前血清IGF-Ⅰ、Ⅱ和IGFBP3、5分别为985.7μg/L、1154.0μg/L和46.6 μg/L、9.6 μg/L,术后分别为431.4μg/L、632.6μg/L和26.7 μg/L、6.7μg/L差异均有统计学意义(P值分别为0.001、0.009、0.001、0.002);肾积水组IGF-Ⅰ、Ⅱ和IGFBP3、5水平术前术后比较,差异无统计学意义(P＞0.05).结论 IGF-Ⅰ、Ⅱ和IGFBP3、5在肾透明细胞癌患者的血液中呈现高表达,IGF-Ⅱ具有临床诊断意义.

Abstract：

Objective To observe the expressions of serum insulin-like growth factor (IGF)- Ⅰ ,Ⅱ and IGF binding protein (IGFBP) 3, 5 and to explore the clinical significances in patients with clear cell carcinoma of kidney. Methods Enzyme-linked immunosorbent assay (ELISA) methods were adopted to examine serum expressions of IGF-Ⅰ , Ⅱ and IGFBP 3, 5 in 40 cases with clear cell carcinoma of kidney (renal carcinoma group) and 16 cases with hydronephrosis (control group) from May 2007 to December 2009. Results IGF- Ⅰ , Ⅱ and IGFBP 3,5 in renal carcinoma showed higher expressions before operation (985. 7 μg/L, 1154.0 μg/L,46.6 μg/L and 9.6 μg/L, respectively)than after operation (431.4 μg/L, 632.6 μg/L, 26.7 μg/L, and 6.7 μg/L, respectively, all P＜0. 05 ～0.01). There were no significant differences in those indexes between pre- and post- operation in control group (P＞ 0. 05). Conclusions There are high expressions of serum IGF-Ⅰ , Ⅱ and IGFBP 3, 5 in renal carcinoma patients, and IGF- Ⅱ has clinical significance in diagnosis.     

Effects of different levels of iodine concentration on insulin-like growth factors Ⅰ mRNA expression of thyroid and breast in lactating rats

Objective To study the effects of different levels of iodine concentration on insulin-like growth factors Ⅰ (IGF-1) mRNA expression of thyroid and breast in lactating rats. Methods Thirty Wistar female rats, having been weaned for 1 month, were randomly divided into three groups according to their body weights, i. e. :low iodine(LI) group,adequate iodine(AI) group, high iodine(HI) group, 10 rats in each group. Synthetic fodder and deionized water containing iodine of 0,150,3000 μg/L was respectively fed to these rats. After fed for 3 months, the rats mated and had offspring. Their mammary glands, thyroids and serum were sampled at lactation day 5. The serum iodine of lactating rats were determined by moderate acid digestion method, level of T3 and T4 were determined by radioimmunoassay method, and the expressions of IGF-1 mRNA of mammary glands and thyroids were determined by real-time fluorescence quantitative PCR assay. Results The value of serum iodine of LI group [(17.38±3.27) μg/L] was lower than that of AI group [(43.42±6.92) μg/L, P<0.05], and the value of serum iodine of HI group[(350.10±38.46)μg/L] was higher than that of AI group (P<0.05). The level of T3 of LI group and HI group[ (1.11±0.25), (1.61±0.33)μg/L] reduced obviously compared with that of AI group[(2.18±0.46) μg/L, P<0.05]. The mean of T4 of LI group and HI group[(33.40±11.11),(56.54±10.38)μg/L] had no statistical significance compared with AI group(44.02±12.51)μg/L, P>0.05), but the level of T4 of LI group was lower than that of HI group(P<0.05). The level of IGF-1 mRNA expression of thyroid in LI group and HI group (0.34±0.08, 0.23±0.08) was higher than that of AI group(0.15±0.03, P<0.05). The level of IGF-1 mRNA expression of lactating mammary in LI group(0.59±0.18) was higher than that of AI group(0.40±0.10, P<0.05). The level of IGF-1 mRNA expression of thyroid was lower than that of breast between the same group(t=3.54, 6.44,2.62, all P<0.05). Conclusion Iodine could affect IGF-1 mRNA expression of thyroid and lactating mammary, and IGF-1 mRNA expression of lactating mammary was stronger than that of the thyroid.     

血浆甘露聚糖结合凝集素与类风湿关节炎肾损害的相关性研究

目的 检测类风湿关节炎(RA)相关性肾损害患者甘露聚糖结合凝集素(MBL)的血浆水平,探讨MBL在RA相关肾损害中可能的作用机制.方法 用酶联免疫吸附试验(ELISA)法检测19例RA相关肾损害患者、49例RA不伴肾损害患者及40名健康对照者的血浆MBL水平,并收集RA患者临床资料及相关实验室检查资料进行对比分析.采用x2检验、t检验和Spearman相关分析进行统计学处理.结果 较无肾损害组比,RA相关肾损害组患者肿胀压痛关节数增多[(15±9)和(9±11)个],晨僵时间延长[(2.9±1.3)和(2.3±1.6)h],其他(除肾脏)关节外伴随症状发生率明显升高[(42.1%和16.3%),P＜0.01或P＜0.05];但二组间RA病程、关节畸形的发生率差异无统计学意义(P＞0.05);RA相关肾损害组血小板(PLT)[(376±155)×109/L和(304±121)×109/L]、循环免疫复合物(CIC)[(4.3±3.0)和(2.9±3.3)g/L]、红细胞沉降率(ESR)[(79±46)和(53±31)mm/1 h]、类风湿因子(RF)[(77±42)和(52±49)U/ml]、C反应蛋白(CRP)[(32±28)和(23±18)mg/L]、免疫球蛋白IgG[(11.7±2.6)和(8.4±2.4)g/L]、补体C3[(1.18±0.53)和(0.94±0.21)g/L]高于RA无肾损害组(P＜0.01或P＜0.05),血浆白蛋白(Alb)[(26±13)比(30±9)g/L]低于无肾损害组(P＜0.05);二组RA患者血浆MBL水平均较对照组血浆MBL水平[(3.1±0.5)mg/L]显著下降(P＜0.01),RA相关肾损害组MBL水平[(1.7±1.2)mg/L]较无肾损害组MBL水平[(1.4±1.3)mg/L]升高(P＜0.05);RA相关肾损害组MBL与IgG、C3、CRP、尿蛋白定量(24 h)呈正相关(r分别为0.6,0.6,0.47,0.57;P＜0.05).结论 RA相关肾损害与免疫复合物相关;RA相关肾损害患者血浆MBL水平是升高的,血浆中高水平MBL可能是RA相关肾损害的发病机制之一.

Abstract：

Objective To detect the serum level of mannose binding lectin (MBL) in patients with renal injury induced by rheumatoid arthritis (RA), and to investigate the role of MBL in the pathogenesis of renal injury in RA. Methods ELISA was used to measure the serum MBL level of 19 RA patients with renal injury, 49 RA patients without renal injury and 40 healthy individuals. The clinical features and laboratory markers were compared and analyzed by chi-square test, two independent samples t-test and Spearman's correlation analysis. Results Compared with RA patients without renal injury, the number of tender and swollen joints [(15±9) vs (9±11)], duration of morning stiffness [(2.9±1.3) vs (2.3±1.6) h] and extraarticular manifestations (42.1％ vs 16.3％) in RA patients with renal injury were significantly higher (P＜0.05or P＜0.01). There was no significant difference between the two groups in RA disease duration and jointdeformity(P＞0.05). In patients with renal injury, the level of platelet count [(376±155)×109/L vs (304±121)×109/L], CIC[(4.3±3.0) vs (2.9±3.3) g/L], ESR[(79±46) vs (53±31) mm/1 h], RF[(77±42) vs (52±49)U/ml], CRP[(32±28)vs (23±18)mg/L], IgG[(11.7±2.6)vs (8.4±2.4)g/L], C3[(1.18±0.53)vs (0.94±0.21) g/L] were higher than those in RA patients without renal injury (P＜0.01 or P＜0.05); the level of Alb [(26±13) vs (30±9) g/L] was lower than that in RA patients without renal injury (P＜0.05). The level of serum MBL in the two groups of RA patients was significantly lower than that in the healthy group [(3.1±0.5)mg/L](P＜0.01), and the level of serum MBL in RA patients with renal injury [(1.7±1.2) mg/L] was higher than that in RA patients without renal injury [(1.4±1.3) mg/L](P＜0.05). The level of serum MBL in RA patients with renal injury showed a positive correlation with IgG, C3, CRP and 24 h urine protein level (r=0.6, 0.6, 0.47, 0.57; P＜0.05). Conclusion Renal injury in RA patients is immune complex dependent. The serum level of MBL is higher in patients with renal injury, therefore, high-concentration MBL may be one of a potential causes of renal injury in RA patients.     

胰岛素样生长因子基因在非酒精性脂肪变性肝细胞模型中的表达及其意义

目的 研究胰岛素样生长因子-1 (IGF-1)及胰岛素样生长因子结合蛋白-3(IGFBP-3)基因在非酒精性脂肪变性肝细胞模型中的表达变化及其意义. 方法 用油酸诱导永生化人肝细胞(IHH)建立非酒精性脂肪性肝病(NAFLD)细胞模型,油红O染色和细胞内甘油三酯含量检测观察细胞脂肪变情况.IHH细胞分为对照组和NAFLD组,对照组细胞以DMEM/F12培养基培养,NAFLD组给予油酸0.5 mmol/L处理72 h.采用逆转录酶-聚合酶链反应及Western blot和免疫荧光染色方法检测IGF-1和IGFBP-3在两组细胞中的mRNA及蛋白质表达变化.组间均数比较采用t检验. 结果 0.5 mmol/L油酸可成功诱导IHH细胞脂肪变性,油红O染色显示细胞内脂肪滴明显增多,细胞内甘油三酯含量从对照组的(150.2±15.6)μg/ng升高到(275.7±27.2) μg/mg (t=21.67,P＜0.01).油酸诱导后,NAFLD组细胞中IGF-1和IGFBP-3的mRNA相对表达量(分.别为0.76±0.04和1.58±0.93)均较对照组(分别为4.82±1.51和5.41±1.37)明显下降,t值分别为17.915和12.893,P值均＜0.01;IGF-1和IGFBP-3的蛋白质相对表达量(分别为1.00±0.29和0.65±0.36)也较对照组(分别为2.56±0.71和1.23±0.91)明显下降,t值分别为29.17和32.12,P值均＜0.01.免疫荧光染色结果也证实NAFLD组细胞中IGF-1和IGFBP-3的蛋白质表达较对照组明显下降. 结论 非酒精性脂肪变性肝细胞模型的IGF-1和IGFBP-3表达下降,为深入研究临床上部分非酒精性脂肪肝病儿童身高受限的机制提供了实验基础.     

不同碘营养水平对哺乳期大鼠甲状腺和乳腺胰岛素样生长因子Ⅰ mRNA表达的影响

目的 观察不同碘营养水平对哺乳期大鼠甲状腺和乳腺胰岛素样生长因子Ⅰ(IGF-1)mRNA表达水平的影响.方法 30只雌性断乳1个月Wistar大鼠按体质量随机分成3组:低碘组、适碘组、高碘组,每组10只,食用合成饲料,分别饮用含碘0、150、3000 μg/L的去离子水.喂养3个月后,与雄鼠合笼交配,待母鼠哺乳5 d后将其处死、取母鼠乳腺、甲状腺及血清,温和酸消化法测定血清碘,放射免疫分析法测定T3、T4水平,实时荧光定量PCR法检测乳腺和甲状腺IGF-1 mRNA表达水平.结果 低碘组血清碘[(17.38±3.27)μg/L]低于适碘组[(43.42±6.92)μg/L,P<0.05],高碘组血清碘[(350.10±38.46)μg/L]高于适碘组(P<0.05);低碘组、高碘组T3水平[(1.11μ0.25)、(1.61±0.33)μg/L]低于适碘组[(2.18±0.46)μg/L,P均<0.05];低碘组、高碘组T4水平[(33.40±11.11)、(56.54±10.38)μg/L]与适碘组[(44.02±12.51)μg/L]比较差异无统计学意义(P>0.05),低碘组T4水平低于高碘组(P均<0.05);低碘组、高碘组甲状腺IGF-1 mRNA表达水平(0.34±0.08、0.23±0.08)均高于适碘组(0.15±0.03,P均<0.05);低碘组哺乳期乳腺IGF-1 mRNA表达水平(0.59±0.18)高于适碘组(0.40±0.10,P<0.05);3组甲状腺IGF-1 mRNA表达均低于同组的乳腺表达水平(t=3.54、6.44、2.62,P均<0.05).结论 碘能够影响哺乳期甲状腺和乳腺IGF-1 mRNA的表达,且哺乳期乳腺表达强于甲状腺.     

结节性甲状腺肿患者血清胰岛素样生长因子-1水平与摄131碘率相关性研究

目的 研究结节性甲状腺肿患者血清胰岛素样生长因子-1(IGF-1)水平与甲状腺摄131碘(131Ⅰ)率的相关性,为临床鉴别甲状腺结节的性质寻找更简捷,安全的方法 .方法 运用放射核素扫描技术检测甲状腺摄131Ⅰ率,据此将患者分为热结节组,冷结节组,并设对照组,放射免疫分析法检测各组血清IGF-1,FT3,FT4,sTSH水平,分析其与摄131Ⅰ率的相关性.结果 热结节组患者血清IGF-1,FT3,FT4水平[(315.86±22.74)μg/L,(9.95±5.62),(67.27±27.31)ng/L]明显高于对照组[(256.13±39.85)μg/L,(2.80±1.30),(13.5l±5.50)ng/L],摄131Ⅰ率(0.64±0.17)明显高于对照组(0.35±0.15),血清sTSH水平[(0.35±0.03)mU/L]明显低于对照组[(2.71±1.17)mU/L].差异均有统计学意义(P＜0.01).而冷结节组患者血清IGF-1,FT3,FT4,sTSH水平[(263.17±30.23)μg/L,(2.89±0.98),(14.23±2.84)ng/L,(2.81±0.42)mU/L]与对照组比较差异无统计学意义(P＞0.05).热结节组IGF-1水平与甲状腺摄131Ⅰ率呈正相关(r＝0.835,P＜0.01),与sTSH水平呈轻度负相关(r＝-0.326,P＜0.05).结论 结节性甲状腺肿患者血清IGF-1水平与甲状腺摄131Ⅰ率可能有一定的相天性.     

胰岛素样生长因子及其结合蛋白与2型糖尿病肾脏病变相关性研究

目的探讨胰岛素样生长因子(IGF)及其结合蛋白(IGFBP)在2型糖尿病肾脏病变中的变化及其相关关系。方法2000-02～2003-05对广东省人民医院的130例(男52例、女78例),年龄在37～75岁2型糖尿病患者,观察了IGFs、IGFBPs、血脂、空腹和餐后2hC肽(CP0、CP120)等与血、尿α1和β2-微球蛋白(MG)的相关性,比较不同肾脏病变组间的IGFs和IGFBPs改变。结果多元逐步回归分析,血α1-MG主要相关预测因子为IGF-2、TC、年龄(r=0.492,P=0.0001);尿α1-MG为IGF-2、年龄(r=0.307,P=0.007);血β2-MG为IGFBP-1、HDL、年龄(r=0.528,P=0.0001);尿β2-MG为IGFBP-1、CP120、年龄(r=0.407,P=0.0001);血肌酐(Cr)为IGFBP-1、CP0、年龄(r=0.499,P=0.0001)。IGF-2和IGFBP-1在糖尿病肾病组显著升高[IGF-2:(889.48±65.94)μg/L对(711.57±28.73)μg/L,P=0.03;IGFBP-1:(94.91±17.48)μg/L对(54.81±5.04)μg/L,P=0.009]。结论2型糖尿病患者血清IGFBP-1、IGF-2水平与血、尿微球蛋白水平有密切的相关性,糖尿病肾病的IGFBP-1和IGF-2明显升高。糖尿病的胰岛功能改变、IGF与IGFBP系统以及脂代谢异常,与糖尿病肾病的发生、发展有关。     

非霍奇金淋巴瘤患者血清胰岛素样生长因子-1及其结合蛋白-3表达水平的初步观察

目的:探讨非霍奇金淋巴瘤(NHL)患者血清胰岛素样生长因子-1(IGF-1)及其结合蛋白-3(IGFBP-3)表达水平及其临床意义.方法:选择28例诊断初发NHL患者(淋巴瘤组)及28例健康志愿者(对照组),化学发光法测定血清IGF-1及IGFBP-3水平并计算IGF- 1/IGFBP-3值,分析组间的差异.结果:血清IGF-1及IGFBP-3水平淋巴瘤组显著低于正常对照组(均P＜0.01);IGF-1/IGFBP-3淋巴瘤组与正常对照组差异无统计学意义(P＞0.05);不同亚型的淋巴瘤组的血清IGF-1、IGFBP-3水平及IGF-1/IGFBP-3比值的差异均未达到统计学意义(P＞0.05).结论:N HL患者血清IGF-I及IGFBP-3水平明显降低,可能与NHL相关.IGF-1/IGFBP-3比值与NHL无明显相关性,IGF-1及IGFBP-3水平与NHL的分型无明显相关.     

原发性干燥综合征90例血清C反应蛋白转化生长因子-β1水平变化及意义

目的:探讨血清CRP、TGF-β1与pSS的联系。方法:选择90例pSS患者(观察组)及30名健康人群(对照组),观察组按病情严重不同分为重度17例、中度43例、轻度30例,观察组中未发生肺间质改变(ILD)54例、合并ILD 36例(其中Ⅰ期患者15例、Ⅱ期患者13例、Ⅲ期患者8例)。ELISA测定患者和健康人血清中CRP、TGF-β1水平,并探讨其和pSS严重程度及ILD不同分期的联系。统计方法使用方差分析,SNK-q检验或t检验,Spearman等级相关分析等。结果:观察组血清CRP、TGF-β1水平分别为[(13.5±7.8)mg/L],[(61±14)μg/L],高于对照组[(3.5±1.1)mg/L]和[(28±9)μg/L],差异有统计学意义(t=6.980,P<0.01;t=12.086,P<0.01)。在观察组内,病情中、重度的患者其血清CRP浓度分别为[(14.6±2.1)mg/L]和[(16.5±3.3)mg/L],比轻度患者[(11.3±3.2)mg/L]高,病情中、重度的患者其血清TGF-β1浓度分别为[(61±13)μg/L]和[(76±18)μg/L],比轻度患者[(51±14)μg/L]高,而且重度患者的水平提高的更显著(F=3.634,P<0.01;F=4.661,P<0.01);血清CRP、TGF-β1浓度和病情严重度呈正相关[r=0.786,P<0.01;r=0.516,P<0.01]。观察组内患者合并有ILD的患者,其血清CRP、TGF-β1浓度分别为[(15.5±3.2)mg/L]和[(74±19)μg/L],比无合并ILD的患者[(10.5±2.2)mg/L]和[(52±14)μg/L)]高(t=8.791,P<0.01;t=6.321,P<0.01)。观察组内,ILDⅡ、Ⅲ期的患者,其血清CRP浓度分别为[(16.3±8.2)mg/L]和[(19.1±10.1)mg/L],比Ⅰ期患者[(13.4±7.3)mg/L]高,TGF-β1浓度分别为[(74±19)μg/L]和[(85±21)μg/L],比Ⅰ期患者[(63±15)μg/L]高,Ⅲ期的患者水平高的更显著(F=4.640,P<0.01;F=5.673,P<0.01);pSS患者血清CRP、TGF-β1的浓度和ILD的分期都呈正相关关系(r=0.718,P<0.01;r=0.809,P<0.01)。结论:pSS患者血清CRP、TGF-β1浓度都较健康人升高,这2种因子很可能和pSS病情活动以及合并的ILD的转归有联系。     

Role of growth hormone, insulin-like growth factor 1 and insulin-like growth factor-binding protein 3 in development of non-alcoholic fatty liver disease

Background and aims Pituitary dysfunction including growth hormone (GH) deficiency may be associated with non-alcoholic fatty liver disease (NAFLD). Since the relationships among GH, IGF-1, IGFBP-3, and development of NAFLD without hypopituitarism are unclear, we examined the role of these hormones in the development of NAFLD based on clinical, laboratory and liver histology data. Patients and methods A total of 55 consecutive patients (20 males and 35 females) with NAFLD. Results Aspartate amino transferase (AST), AST/ALT, platelet count and IGF-1, levels were significantly associated with differences in fibrosis, since these variables differed between stage 0–1 and stage 2–3 NAFLD. In multivariate analysis, platelet count (P = 0.0223, relative risk (RR), 5.899; 95% confidence interval (CI), 1.288–27.017), and IGF-1 (P = 0.0363, RR, 4.568; 95% CI, 1.101–18.945) showed significant associations with stage 2–3 NAFLD. Additionally, hyaluronic acid levels had a negative relationship with IGF-1 and the IGF-1/IGFBP-3 ratio. There was no relationship of fibrosis with GH level, but decreased GH (P = 0.0414, RR, 0.199; 95% CI, 0.042–0.989) was significantly associated with steatosis of stage 2–3. Low GH/IGF-1 and GH/IGFBP-3 ratios were found in advanced steatosis. Conclusion GH, IGF-1 and IGFBP-3 are associated with hepatic fibrosis and steatosis in NAFLD. Low levels of IGF-1 might be associated with fibrosis while low level of GH with hepatic steatosis.     

Possible roles of insulin,IGF-1 and IGFBPs in initiation and progression of colorectal cancer

AIM: To investigate the roles of serum insulin, insulinlike growth factor-1(IGF-1), and insulin-like growth factor binding proteins(IGFBPs) in the initiation and progression of colorectal cancer. METHODS: We determined serum insulin, IGF-1 and IGFBPs levels in 615 colorectal cancer patients and 650 control healthy donors by enzyme-linked immunosorbent assay(ELISA). In the meantime, their body mass index(BMI) and waist-to-hip ratio(WHR) were measured. RESULTS: Serum levels of insulin and IGF-1 as well as IGF-1/IGFBP-3 ratio in pre-operation patients were significantly elevated, but the level of IGFBP-3 was significantly decreased compared with normal controls and post-operation patients(P < 0.05 and P < 0.001,respectively). There is no significant difference(P > 0.05) in the levels of insulin, IGF-1, IGFBP-1, IGFBP-3 and IGF-1/IGFBP-3 between the patients with and without hepatic as well as distal abdominal metastases. WHR and BMI of colon cancer patients were positively and significantly correlated with the levels of insulin and IGF-1/IGFBP-3. In contrast, WHR and BMI were negatively correlated with IGFBP-3 level. CONCLUSION: The elevation of insulin, IGF-1 as well as IGF-1/IGFBP-3 ratio and the reduction of IGFBP-3 may be related to the initiation of colorectal cancer, but they are not related to the progression and outcome of the disease.     

老年住院病人血清胰岛素样生长因子-1、胰岛素样生长因子结合蛋白-3与衰弱的相关性研究

目的探讨老年住院病人血清胰岛素样生长因子-1(IGF-1)、胰岛素样生长因子结合蛋白-3(IGFBP-3)水平与衰弱的相关性。方法纳入2018年1月至2019年6月老年医学科年龄≥65岁的住院病人195例,进行衰弱表型评估和老年综合评估,采用ELISA法检测血清IGF-1、IGFBP-3水平,分析其与衰弱的相关性。结果与无衰弱、衰弱前期病人相比,衰弱病人血清IGF-1、IGFBP-3水平显著下降,差异有统计学意义(P<0.05)。有序多分类Logistic回归分析显示血清IGF-1(OR=0.943,95%CI0.894~0.994,P<0.05)、IGFBP-3(OR=0.397,95%CI0.259~0.607,P<0.001)水平与衰弱显著相关。结论血清IGF-1、IGFBP-3水平与老年住院病人衰弱显著相关,可能成为老年人衰弱的预测指标。     

急性冠状动脉综合征患者载脂蛋白A5检测及其意义

目的 探讨血清载脂蛋白A5(APOA5)水平与急性冠状动脉综合征(ACS)患者血脂谱及高敏性C反应蛋白(hs-CRP)的关系.方法 入选587例受试对象,分为对照组(n=232)、稳定性心绞痛(SA)组(n=127)、不稳定性心绞痛(UA)组(n=116)和急性心肌梗死(AMI)组(n=112).抽血分离血清,分别采用ELISA法和免疫比浊法测定血清APOA5以及hs-CRP,并测定血清甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C).比较各组ApoA5、hs-CRP和血脂的血清浓度差异,分析三者之间的相互关系.结果 UA组[(340.6±63.5)μg/L]和AMI组[(373.2±73.8)μg/L]的ApoA5浓度明显高于对照组[(108.7±23.2)μg/L]和SA组[(78.3±20.2)μg/L](均P<0.05).UA组和AMI组的ApoA5与TG(r=0.63和0.67,P<0.05)和hs-CRP(r=0.57和0.55,P<0.05)呈正相关,而ApoA5与TC、HDL-c和LDL-C无明显相关(P>0.05).结论 ACS患者中血清ApoA5浓度显著升高,与血清TG或hs-CRP浓度显著正相关,提示ApoA5的代谢和生理功能在ACS期间均发生了变化,这可能与炎症反应增强有关.