甘草酸铋抗实验性胃溃疡作用及机制研究

目的：研究甘草酸铋抗实验性胃溃疡的作用及其作用机制。方法：以SD大鼠和昆明种小鼠为研究对象,复制3种实验性胃溃疡动物模型(大鼠幽门结扎致胃溃疡模型、大鼠乙醇致胃溃疡模型和小鼠束缚水浸应激致胃溃疡模型)。将大鼠分成甘草酸铋700,350,175 mg·kg^-1剂量组、甲氰咪胍(200 mg·kg^-1)阳性对照组和0.1%EDTA空白对照组；小鼠分成甘草酸铋980,490,250 mg·kg^-1剂量组。测定胃黏膜损伤指数、胃液分泌量,胃液酸度,血清中一氧化氮(NO)含量,胃组织前列腺素(PGE₂)相对含量；结果：与模型对照组比较,甘草酸铋能使胃液量、胃酸浓度 、胃黏膜损伤指数减少(或降低)；血清NO含量和胃组织前列腺素含量增加。结论：甘草酸铋对实验性胃溃疡具有一定疗效,其作用机制可能与抑制胃酸分泌、改善胃黏膜微循环有关。     

Effect of garlic oil on ethanol induced gastric ulcers in rats

Garlic oil was evaluated for gastroprotective activity against ethanol induced ulcers. Reactive oxygen species are involved in the pathogenesis of these ulcers. The possible involvement of garlic oil in restraining the oxidation process produced in gastric tissue was also investigated. The ulcer index, lipid peroxidation and antioxidant enzyme activity (GPx, catalase, SOD) were determined. Pretreatment with garlic oil in doses of 0.25 and 0.5 mg/kg, 30 min before administration of ethanol (1 mL of 100%) caused a decrease in ulcer index and lipid peroxidation and ameliorated the decrease in antioxidant enzyme levels caused by ethanol. The result suggests that garlic oil possesses antioxidant properties and provides protection against ethanol induced gastric injury.     

甘草酸铋钾对大鼠实验性胃溃疡的作用

目的 研究甘草酸铋钾对胃溃疡的影响。方法 用醋酸性胃溃疡、应急性胃溃疡及幽门结扎性胃溃疡模型测定甘草酸铋钾对溃疡面积、胃液量、胃液酸度和胃蛋白酶的影响。结果 甘草酸铋钾能使溃疡面积和胃液量减少,降低胃液酸度和胃蛋白酶活性。结论 甘草酸铋钾能有效地治疗胃溃疡。     

胃疡宁丸抗实验性胃溃疡的药效及机制研究

目的:研究胃疡宁丸抗实验性胃溃疡的药效及其机制.方法:采用大鼠醋酸注射法、水浸应激性、阿司匹林诱发及幽门结扎性胃溃疡模型,观察胃疡宁丸抗胃溃疡的作用并考察其对血清NO,EGF含量,胃黏膜血流量,PGE2含量,及对胃液分泌、胃酸含量、胃蛋白酶活性的影响.结果:胃疡宁丸能够降低各种胃溃疡模型的溃疡指数,升高NO,EGF含量,PGE2含量及胃黏膜血流量,抑制胃液分泌和胃酸含量及降低胃蛋白酶活性.结论:胃疡宁丸具有明显的抗实验性胃溃疡的作用,该作用与其减少胃黏膜损伤因子(胃酸、胃蛋白酶),增加胃黏膜屏障的功能,增强胃黏膜修复作用(NO,EGF,PGE2,胃黏膜血流量)有关.     

芦荟多糖对动物实验性胃溃疡的影响

目的研究芦荟多糖对动物实验性胃溃疡的影响。方法应用大鼠幽门结扎型胃溃疡模型、大鼠醋酸烧灼型胃溃疡模型、小鼠利血平致胃溃疡模型,观察芦荟多糖ig给药对实验性胃溃疡的预防与治疗作用及对大鼠胃液、胃酸、胃黏液、胃蛋白酶分泌及活性的影响。结果芦荟多糖对实验性胃溃疡具有明显的预防及治疗作用,并能抑制胃液分泌、胃酸分泌及减少胃蛋白酶、胃黏液的分泌,但对胃蛋白酶活性无明显影响。结论芦荟多糖对动物实验性胃溃疡具有良好的预防及治疗作用,并能抑制溃疡致病因子胃酸及胃蛋白酶的分泌。     

野甘草乙醇提取物治疗大鼠胃溃疡作用的研究

目的研究野甘草乙醇提取物对胃溃疡的治疗作用。方法通过醋酸烧灼法制成大鼠胃溃疡模型,按照同窝同性、体质量配对分为2组,实验组每只以野甘草乙醇提取物1 g/(kg.d)喂养,共喂30 d;对照组不喂药。30 d后剖腹取胃,测定溃疡体积。结果实验组大鼠胃溃疡体积小于对照组(P<0.05)。结论野甘草乙醇提取物对大鼠胃溃疡有治疗作用,应对其药理作用进行更深入的研究。     

dl-四氢巴马汀抗大鼠胃溃疡作用

 目的研究dl-四氢巴马汀(dl-THP)对大鼠急、慢性溃疡的保护作用,并初步分析其机制。方法胃黏膜损伤由吲哚美辛、束缚应激、结扎幽门和胃浆膜下注入醋酸引起。dl-THP15,30,45mg·kg^-1灌胃,1日2次,共5次。测定大鼠胃溃疡数目、面积、胃酸分泌、胃蛋白酶活性以及胃黏膜血流。结果dl-THP15,30,45mg·kg^-1能抑制大鼠胃黏膜损伤,且呈剂量依赖性;dl-THP30mg·kg^-1对大鼠慢性胃溃疡的抑制率为75.8%,与雷尼替丁30mg·kg^-1的67.7%相仿。dl-THP可增加胃黏膜血流,但对胃酸和胃蛋白酶分泌无影响。结论dl-THP具有明显抗实验性胃溃疡作用,可能与其增加胃黏膜血流有关,亦可能部分通过阻滞胃黏膜DA受体而发挥作用。     

Ulcer protective effect of Terminalia arjuna on gastric mucosal defensive mechanism in experimental rats

The methanol extract of the bark of Terminalia arjuna (Combretaceae) (TAE) showed marked antiulcer and ulcer healing activity against 80% ethanol (ETH), diclofenac sodium (DIC) and dexamethasone (DEX) induced ulcer models dose dependently at doses of 100, 400 and 200 mg/kg body weight respectively. Pre-, post and co-administration of TAE offered 100% protection to the gastric mucosa against ETH, DIC and DEX induced ulcers as observed from the ulcer score. Gastric mucosal analysis of DEX induced rats were associated with changes in the levels of protein, protein bound carbohydrate complexes, lipid peroxides (LPO), glutathione (GSH) and activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) compared with control rats. Co-administration with TAE in DEX rats (DEX + TAE) favorably altered the levels of LPO, GSH and also the activities of SOD and CAT in gastric mucosa, whereas the activities of GPx remained unaltered in all groups. In DEX + TAE rats, the levels of protein and protein bound carbohydrate complexes were increased when compared with DEX rats. The results indicate that the gastroprotective effect of TAE is probably related to its ability to maintain the membrane integrity by its antilipid peroxidative activity that protects the gastric mucosa against oxidative damage and its ability to strengthen the mucosal barrier, the first line of defense against exogenous and endogenous ulcerogenic agents.     

加味黄芪建中汤对大鼠乙酸胃溃疡胃黏膜的保护作用

目的探讨加味黄芪建中汤对胃黏膜的保护作用机制。方法将60只W istar大鼠随机分成,正常对照组、模型组、加味黄芪建中汤大剂量组(20 g/kg)、加味黄芪建中汤中剂量组(10 g/kg)、加味黄芪建中汤小剂量组(5 g/kg)和果胶铋组各10只,采用100%乙酸诱导的慢性胃溃疡模型,给予相应治疗16 d后,观察胃黏膜的病理、血液中前列腺素(6-K-PGF1α)、胃液中表皮生长因子(EGF)、胃组织中一氧化氮(NO)及血液中超氧化歧化酶(SOD)变化。结果加味黄芪建中汤促进了胃溃疡黏膜修复、显著提高了血浆6-k-PGF1α、胃液中EGF、胃组织中NO及血浆SOD水平。结论加味黄芪建中汤对大鼠乙酸胃溃疡胃黏膜有明显的保护作用。     

健胃愈疡颗粒对胃溃疡患者胃黏膜hTFF1mRNA表达的影响

目的　探讨健胃愈疡颗粒 (JWYY)对胃溃疡 (GU)患者胃黏膜hTFF1mRNA表达的影响 ,揭示其抗溃疡复发的分子机制。方法　将 60例胃镜下确诊的GU (中医辨证为肝郁脾虚证 )患者 ,随机分为JWYY治疗组和西药对照组 ,每组 3 0例 ;另设胃镜下无胃黏膜病变的患者 10例为正常组。两组GU患者第 1个疗程结束前后均于胃镜下取溃疡边缘组织活检标本 2块 ,迅速置液氮 -80℃保存并行RT -PCR检测。结果　JWYY能提高人胃黏膜hTFF1mRNA表达 ,两组经统计学处理无显著性差异。结论　JWYY能增强人胃黏膜hTFF1mRNA的上增性表达 ,从而提高溃疡愈合质量 ,是JWYY抗胃溃疡复发作用的部分机制     

The antiulcer activity of Garcinia cambogia extract against indomethacin-induced gastric ulcer in rats.

Garcinia cambogia extract is a herbal preparation that has been suggested as useful in the treatment of gastrointestinal disorders. In the present study this drug was tested for its antiulcerogenic effect. Oral pretreatment with Garcinia cambogia fruit extract (1 g/kg body wt/day) for 5, 10 or 15 days protected the gastric mucosa against the damage induced by indomethacin (20 mg/kg body wt). The volume and acidity of the gastric juice decreased in the pretreated rats. The glycoprotein levels of the gastric contents which were decreased in the untreated rats, maintained near normal levels in the pretreated rats. Protein which was elevated in the gastric juice of untreated rats, showed near normal levels in the pretreated rats. Garcinia cambogia was able to decrease the acidity and to increase the mucosal defence in the gastric areas, thereby justifying its use as an antiulcerogenic agent.     

苗药一支箭乙醇提取物对乙酸型胃溃疡大鼠EGF表达的影响

目的：研究一支箭乙醇提取物对乙酸型胃溃疡大鼠的作用,并初步探讨其促进溃疡愈合的机制。方法：建立大鼠乙酸型胃溃疡模型,观察一支箭乙醇提取物对大鼠乙酸型胃溃疡的作用;采用免疫组化染色法,观察一支箭乙醇提取物对大鼠乙酸型胃溃疡粘膜EGF表达的影响。结果：EEO能明显促进大鼠乙酸型胃溃疡的愈合,溃疡愈合率可达到62.5%;EEO能明显促进大鼠乙酸型胃溃疡边缘组织EGF的表达。结论：EEO能明显促进大鼠乙酸型胃溃疡的愈合,其机制可能与溃疡边缘组织EGF的表达有关。     

Opuntia ficus indica (L.) Mill. mucilages show cytoprotective effect on gastric mucosa in rat

Opuntia ficus indica cladodes possess a protective action against ethanol-induced ulcer in the rat. The major components of cladodes are carbohydrate polymers, mainly mucilages and pectin. To clarify the cytoprotective effects of cladodes on experimental ethanol-induced ulcer in rat, mucilages and pectin were extracted and were administered instead of cladodes. The above mentioned effects induced by cladodes may be attributed to mucilages, and not significantly to pectin.     

Attenuation of indomethacin- and HCl/ethanol-induced oxidative gastric mucosa damage in rats by kolaviron, a natural biflavonoid of Garcinia kola seed

Reactive oxygen species (ROS) have been implicated in the aetiology of HCl/ethanol- and indomethacin gastric mucosal damage. This study investigated the protective effects of kolaviron, a natural antioxidant from the seed of Garcinia kola, on oxidative gastric mucosal damage induced by HCl/ethanol, and indomethacin.A HCl/ethanol mixture (1.5 mL of 0.15 n HCl in 70% ethanol) and indomethacin (IND) caused severe gastric damage with an ulcer index of 2.90 +/- 0.8 and 2.5 +/- 0.4, respectively, and significant reductions in the gastric mucosal content of catalase (CAT), superoxide dismutase (SOD) and glutathione (GSH) (p < 0.001).Pre-treatment of animals with kolaviron (100 mg/kg) orally 1 h and once daily for 3 days prior to ulcer induction significantly reduced the formation of ulcers induced by HCl/ethanol with preventive ratios of 65 and 72, respectively, while rats treated with kolaviron 1 day and for 7 days prior to IND treatment attenuated ulcer formation by 59% and 77%. Pre-treatment with ranitidine 1 h prior to ulcer induction (50 mg/kg) elicited preventive ulcer ratio of 55.Kolaviron pre-treatment 1 h before ulcer induction attenuated the HCl/ethanol reduction in CAT, SOD and GSH by 43%, 42% and 30%, respectively, and 67%, 68% and 64% following 72 h treatment with kolaviron. Ranitidine elicited 24%, 41% and 29% protective effects, respectively.Similarly, kolaviron administered to rats 1 day and for 7 days before IND treatment attenuated the drug-induced inhibition of CAT by 44% and 70%; SOD by 23% and 43% and GSH by 32% and 55%, respectively.In a 1 h and 3-day treatment with kolaviron before HCl/ethanol administration, MDA was reduced by 35% and 55%, respectively, while kolaviron administration 1 day and for 7 days before IND elicited a 39% and 58% reduction in MDA. Ranitidine elicited 39% and 50% reduction in MDA following HCl/ethanol and IND treatment.The results indicate the gastroprotective activity of kolaviron, which may be linked to its intrinsic antioxidant properties.     

Guarana (Paullinia cupana Mart.) offers protection against gastric lesions induced by ethanol and indomethacin in rats

The effects of guarana (Paullinia cupana) extract were analyzed in rats on acute gastric lesions induced by ethanol and indomethacin and were compared to those produced by caffeine, a methylxanthine. Guarana (50 and 100 mg/kg p.o.) pretreated animals showed a significant reduction in the severity of gastric lesions induced by absolute ethanol in a manner similar to caffeine (20 and 30 mg/kg p.o.). Against indomethacin-induced gastric ulceration, guarana at a higher dose offered significant protection but caffeine was ineffective at the doses tested. In 4 h pylorus-ligated rats, both guarana and caffeine caused significant diminution in the gastric secretory volume as well as the total acidity. Gastrointestinal transit in mice was not significantly affected by either of these agents. These findings indicate that guarana has a gastroprotective property that needs further elucidation as regards to its mechanism.     

The aerial parts of Guazuma ulmifolia Lam. protect against NSAID-induced gastric lesions

Guazuma ulmifolia Lam., a member of the Sterculiaceae family, is used in folk medicine because of its antioxidant, antimicrobial and antihypertensive properties. Most of the research work carried out on this plant has focused on the bark because of its high concentration of antioxidant proanthocyanidins. The flowers and leaves of Guazuma ulmifolia, though less studied, are also used as a remedy for different conditions, such as kidney and gastrointestinal diseases, fever and diabetes. The aim of this study was to assess the gastroprotective effects of an aqueous suspension of the ethanolic extract from leaves and flowers of Guazuma ulmifolia in a model of acute gastric ulcer induced by diclofenac as ulcerogenic agent, using the proton pump inhibitor omeprazole as a protection reference. Therefore, the extract was administered two times orally to three groups of Wistar rats at doses of 500, 250 and 125mg/kg, with a 24-h interval between doses. Diclofenac (100mg/kg) was given 1h after the last administration of the extract. Pretreatment with Guazuma ulmifolia or omeprazole decreased the ulcerated area in a dose-dependent way. Myeloperoxidase activity as a marker of neutrophil infiltration was slightly reduced in vivo, whereas in vitro, anti-inflammatory activity was clearly inhibited in a dose-dependent way. The lowest doses of the extract significantly decreased the levels of lipoperoxides, and superoxide dismuthase activity increased to a similar extent as with omeprazole (P<0.001). Examination of glutathione metabolism reflected a significant rise in glutathione peroxidase activity at the highest dose of Guazuma ulmifolia. Finally, there was a faint elevation in prostaglandin E(2) levels with all doses, though the depletion induced by diclofenac could not be reverted. We conclude that the aerial parts of Guazuma ulmifolia protect gastric mucosa against the injurious effect of NSAIDs mainly by anti-inflammatory and radical-scavenging mechanisms.     

两面针提取物抗氧化作用

为研究两面针３种提取物抗氧化作用,采用比色法测定过氧化脂质以探讨两面针提取脂质过氧化作用,并用化学发光法检测该提取物对大鼠全血化学发光的抑制作用及对碱性连苯三酚体系产生的Ｏ２＾－的清除作用。实验证明,两面针３种提取物对全血化学发光有抑制作用,对由碱 连苯三酚体系产生的Ｏ２＾－有不同程度的清除 ,对由Ｆｅ＾２＋－半胱氨酸诱发的肝脂质过氧化有明显抑制作用。     

Effect of Commiphora opobalsamum (L.) Engl. (Balessan) on experimental gastric ulcers and secretion in rats

The ulcer protective potential of an ethanol extract of Commiphora opobalsamum (L.) Engl. (Burseraceae) 'Balessan' was assessed against different acute gastric ulcer models in rats induced by necrotizing agents (80% ethanol, 0.2M NaOH and 25% NaCl), hypothermic restraint stress, pyloric ligation (Shay) and indomethacin. Balessan, 250 and 500 mg/kg administered orally (intraperitoneally in Shay rat model) showed a dose-dependent ulcer protective effects in all the above ulcer models. Besides, the extract offered protection against ethanol-induced depletion of stomach wall mucus and reduction in nonprotein sulfhydryl (NP-SH) concentration. Ethanol treatment also caused histopathological lesions of the stomach wall. Pretreatment with Balessan extract provided a complete protection of gastric mucosa through supporting both the offensive and defensive factors. Balessan extract was also showed a large margin of safety without any apparent adverse effects in rats.     

七方胃痛胶囊对实验性胃溃疡大鼠表皮生长因子及其受体的影响唐梅文

目的：探讨中药七方胃痛胶囊抗溃疡的机制及提高溃疡愈合质量的应用价值。方法：40只Wistar大鼠制作乙酸诱发大鼠胃渍疡模型后第3天随机分为对照组、雷尼替丁胶囊、七方胃痛胶囊大、小剂量4组。取胃组织测量溃疡面积。观测再生粘膜纽织炎症细胞数、新生血管数，并测定表皮生长因子（EGF）及受体（EGFR）的表达情况。结果：1．七方胃痛胶囊纽溃疡指数明显低于雷尼替丁组和对照组（P〈0．05，P〈0．01）；2．七方胃痛胶囊组的再生粘膜的新生血管数明显高于雷尼替丁组和对照组（P〈0．05，P〈0．01），固有层炎症细胞浸润数明显低于雷尼替丁组和对照组（P〈0．05，P〈0．01）：3．七方胃痛胶囊组溃疡边缘粘膜组织的EGF与EGFR的表达明显高于雷尼替丁组和对照组（P〈0．05，P〈0．01）。结论：七方胃痛胶囊抗溃疡的作用机制与增加EGF与EGFR的表达有关，且能增加再生粘膜的新生血管生成，降低渍疡指数与炎症细胞浸润，对于提高溃疡愈合质量有一定的价值。     

补阳还五汤抗胃溃疡的实验研究

目的 :观察补阳还五汤抗实验性胃溃疡的作用。方法 :采用水应激法、利血平性溃疡法和幽门结扎法建立胃溃疡实验模型。分别观察补阳还五汤 0 .6 g/ kg、1.2 g/ kg不同剂量及甲氰咪胍 0 .2 g/ kg对上述各类型溃疡的抑制作用。结果 :补阳还五汤 0 .6 g/ kg对小鼠应激型溃疡、利血平型溃疡和大鼠幽门结扎型溃疡有明显的抑制作用 (P <0 .0 1或 P <0 .0 5 ) ,对胃蛋白酶活性及排出量则无明显改善 (P >0 .0 5 )。结论 :补阳还五汤可用于治疗胃溃疡。