Computational pharmacology study of Tougu Xiaotong Granule (透骨消痛颗粒) in preventing and treating knee osteoarthritis

Objective： To study the pharmacological properties of Tougu Xiaotong Granule （透骨消痛颗粒, TGXTG） in preventing and treating knee osteoarthritis （KOA） at the molecular level. Methods： The computational methods, including principal component analysis, molecular docking, target-ligand space distribution, and the predictions of absorption, distribution, metabolism, excretion and toxicity （ADMET）, were introduced to characterize the molecules in TGXTG. Results： The structural properties of molecules in TGXTG were more diverse than those of the drug/drug-like molecules, and TGXTG could interact with significant target enzymes related to KOA. In addition, the cluster of effective components was preliminarily identified by the target-ligand space distributions. As to the results of ADMET properties, some of them were unsatisfactory, and were merely regarded as references here. Conclusion： Based on this computational pharmacology study, TGXTG is a broad- spectrum recipe inhibiting many important target enzymes, which could effectively postpone the degeneration of cartilage by coordinately inhibiting the biological effects of cytokines, matrix metallopeptidase 3, and oxygen free radicals.     

Experimental Study of the Inhibitory Effect of Gantai Capsule (肝泰胶囊) on HBsAg and HBeAg Expression

Ｇａｎｔａｉｃａｐｓｕｌｅ（肝泰胶囊，ＧＴＣ）ｉｓａｃａｐ ｓｕｌｅｐｒｅｐａｒａｔｉｏｎｗｈｉｃｈｉｓｂａｓｅｄｏｎｔｈｅｃｌａｓｓｉｃａｌｐｒｅｓｃｒｉｐｔｉｏｎｏｆＭｉｎｏｒＢｕｐｌｅｒｕｍｄｅｃｏｃｔｉｏｎ（小柴胡汤）ａｎｄｓｕｐｐｌｅｍｅｎｔｅｄ     

Summarization on Current Status of the Research on Clinical Application of Xuezhikang Capsule (血脂康胶囊)

XuezhikangCapsule(XZK,血脂康胶囊)isabloodlipidregulatormadeofspecialrefinedredyeast,anditwasprovedthroughlotsofclinicalpracticeinthe10years,tohavesafe,sta bleandevidenteffectinreg ulatingbloodlipids.Thecur rentstatusresearchesonXZKinclinicalapplicationisnowreportedasfollows.EffectsinRegulatingBloodLipids1.BloodlipidsregulatoryeffectonpatientswithprimaryhyperlipidemiaWithpre posttreatmentself controllingorpla cebocontrollingmethodadopted,XZKhasbeenusedtotreatover3000patientsoftheHannation…     

Experimental study on the function of the cricothyreoideus of the dog

ＥｘｐｅｒｉｍｅｎｔａｌｓｔｕｄｙｏｎｔｈｅｆｕｎｃｔｉｏｎｏｆｔｈｅｃｒｉｃｏｔｈｙｒｅｏｉｄｅｕｓｏｆｔｈｅｄｏｇＹｉｎＢａｏｇｕｏ尹保国，ＧｕｏＪｉａｓｏｎｇ郭家松，ＴａｎｇＦｕｂｉｎ唐甫斌，ＺｈａｎｇＲｏｎｇｈａｎ张荣汉ａｎｄＨｕＦａｎｇｌｉ...     

Experimental study on the occlusion of arterial blood flow by the implantation of nitinol alloy stents.

To evaluate the occlusion of arteries, 6 conic intravaseular endoprosthetic stents of titanium-nickel alloy were implanted transluminally to the right external iliac arteries in 2 normal dogs and 4 normal pigs. The stent was characterized by ductility below 313 K (40℃) and restoration memory-shape between 313-319 K. The diameter of the large end of conic stent was 4.0 mm (dog) and 4.5 mm(pig). The topographic anatomy showed that the external iliac artery was near complete occlusion, with a very small residual lumen (about 1 mm in diameter) in dogs at 6 months, with complete occlusion in pigs at 8 months. The histopathological examination indicated that the arterial intima of the upper branch from the stented site was smooth, and that neither inflammatory cell infiltration nor foreign giant cell reaction was found. The newly formed granulation tissue inside the stent came from emboli organization. These results suggest that the shape-memory alloy stent has good biocompatibility and the conic intravascnla     

Clinical and Experimental Study on Treatment of Fatty Liver of Phlegm-Dampness Stagnation Type by Zhiganle (脂肝乐) Capsule

InordertoexploretheeffectofdispellingDampnesstoremovethePhlegm,replenishingQitoactivatethebloodintreatingfattyliver,tofurtherelevatetheleveloftreatingfattyliverwithtraditionalChinesemedicine(TCM),aZhiganlecapsulewasformulated,andclinicalobservationandexperimentalstudywereconductedfromApril1994toMay1996,reportedasfollows.CLINICALSTUDYOlinicalDataOnehundredandninety--threepatientswithfattyliver,82outpatientsand111inpatientswereenrolled.Standardofdiagnosiswasafterlite.at...(1),theTCMSy…     

Chinese Experts Consensus on Clinical Application of Naoxintong Capsule (脑心通胶囊)

正Naoxintong Capsule(脑心通胶囊,NXTC)is derived from the classic formula Buyang Huanwu Decoction(补阳还五汤)by WANG Qing-ren of the Qing Dynasty.Based on the concept ofconcurrent treatment on Nao(Brain)and Xin(Heart)(Nao Xin Tong Zhi)proposed by Prof.ZHAO Bu-chang,activating blood circulation and dredging collaterals herbs were supplemented to the classic formula.NXTC is composed of Astragalus membranaceus(Fisch.)Bge.(monarch     

Clinical Assessment on Treatment of Hyperlipidemia with Pushen Capsule (蒲参胶囊)

Objective: To explore the therapeutic effect of Pushen capsule (PSC, 蒲参胶囊) in treating primary hyperlipidemia. Methods: Two hundred and forty patients with primary hyperlipidemia were randomly divided into two groups, the 120 patients in the treated group treated with PSC (4 capsules, tid) and the 120 patients in the control group treated with Zhibituo tablet (脂必妥, 3 tablets, tid), and they were administered at the same time with Zhibituo placebo. The therapeutic course for both groups was 4 weeks. The therapeutic effect and the effects on blood lipids and viscosity were observed. Results: The effective rate in thetreated group was 76. 3%, which was significantly higher than that in the control group (48. 7% , P< 0. 01). PSC showed a significant lowering effect on TC, TG and LDL-C and raising effect on HDL-C, and the effect in lowering TG was significantly better than that of Zhibituo (P<0.01). PSC also showed a certain effect in decreasing whole blood viscosity of both high-sheared and low-shear     

Effect of Xinfeng Capsule (新风胶囊) on the cardiac function in patients with rheumatoid arthritis

Objective  

To study the changes in cardiac function of rheumatoid arthritis (RA) patients and: to observe the effect of Xinfeng Capsule (新风胶囊, XFC) on them.     

Clinical Study of Effect of Yizhi Capsule(益智胶囊)on Senile Vascular Dementia

Ｃｌｉｎｉｃａｌ　Ｓｔｕｄｙ　ｏｆ　Ｅｆｆｅｃｔ　ｏｆ　Ｙｉｚｈｉ　Ｃａｐｓｕｌｅ（益智胶囊）ｏｎ　Ｓｅｎｉｌｅ　Ｖａｓｃｕｌａｒ　ＤｅｍｅｎｔｉａＣＨＥＮＫａｉ（陈揩），ＣＨＥＮＫｅ－ｊｉ（陈可冀），ＺＨＯＵＷｅｎ－ｑｕａｎ（周文泉），ｅｔａｌ（Ｇ...     

Effects of Quyu Xiaoban Capsule(祛瘀消斑胶囊) on Vascular Endothelial Function in Patients with Atherosclerosis

Objective: To evaluate the therapeutic effects of Quyu Xiaoban Capsule (祛瘀消斑胶囊 ,QYXBC) on endothelial dependent vascular relaxation (EDVR) function in patients with atherosclerosis (AS)with ultrasonic technique. Methods: Tested were the endothelial function and blood lipids of 42 patients with AS in the treated group and 30 healthy volunteers in the control group. And re-examination of these parameters was carried out on the AS patients after they had been treated with QYXBC for 10 months. Results: Before treatment, the reactive hyperemia induced changes in artery diameter in the treated group was significantly lower than that in the control group (P＜0.01), while insignificant difference was found between the two groups in response to nitroglycerin. In the treated group after treatment, with D%-R improved significantly (P＜0.01), total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C)decreased by 16.3%, 5.6%, 10.2% respectively and high-density lipoprotein cholesterol (HDL-C) increased by 7.5%. EDVR was correlated negatively with the serum TC, LDL-C concentrations and the baseline brachial diameter (D0) (r = -0.41, -0.66, -0.59, respectively, all P＜0.01), but correlated positively with HDL-C (r = 0.62, P＜0.05). The ameliorative extent of EDVR was correlated positively to the decreased magnitude of TC and LDL-C concentrations ( r = 0.67,0.59, both P＜0.01 ). Conclusion: QYXBC can lower the level of blood lipids and improve significantly EDVR function.     

Treatment of Non-alcoholic Fatty Liver Disease by Qianggan Capsule(强肝胶囊)

<正>Objective:To observe the therapeutic effect and safety of Qianggan Capsule(强肝胶囊,QGC) in treating non-alcoholic fatty liver disease(NAFLD),using polyene phosphatidylcholine capsule(PPC) as a reference.Methods:Eighty-eight patients with NAFLD were randomly assigned to two groups,45 in the treatment group treated with QGC and 43 in the control group treated with PPC.The course of treatment lasted for 6 months.Changes in liver function,blood lipids,and iconographic indexes before and after treatment were observed,and clinical efficacy was evaluated.Results:In the treatment group,alanine aminotransferase(ALT) was lowered significantly from 56.02±32.59 IU/L before treatment to 38.27±22.68 IU/L after treatment,and CT liver/spleen ratio significantly increased from 0.69±0.18 to 0.91±0.25,showing statistical significance(P0.05);in contrast,the corresponding changes of the two indexes in the control group were 56.56±26.33 IU/L to 49.67±26.22 IU/L, and 0.66±0.20 to 0.75±0.24,respectively,the pre-post treatment difference showing insignificant difference(P0.05). No severe adverse reactions occurred during the whole treatment course.Conclusion:QGC is an effective and safe remedy for the treatment of NAFLD.     

The Effect of Xin'an Capsule(心安胶囊) on Ventricular Arrthythmia in Animals

ＴｈｅｒｅｃｉｐｅｏｆＸｉｎ＇ａｎｃａｐｓｕｌｅ（ＸＡＣ）ｗｉｔｈｔｈｅｍａｉｎｅｆｆｅｃｔｏｆｉｎｖｉｇｏｒａｔｉｎｇＱｉａｎｄｎｏｕｒｉｓｈｉｎｇＹｉｎｗａｓｄｅｓｉｇｎｅｄｓｐｅｃｉａｌｌｙｆｏｒｔｒｅａｔｍｅｎｔｏｆｖｅｎｔｒｉｃｕｌａｒａｒｒｈｙｔｈｍｉａ（ＶＡ）,ａｎｄｉｔｓｄｅｃｏｃｔｉｏｎｆｏｒｍｗａｓｃｏｎｆｉｒｍｅｄｔｏｂｅｅｆｆｅｃｔｉｖｅｉｎｃｌｉｎｉｃａｌｐｒａｃｔｉｃｅ．Ｔｈｉｓｓｔｕｄｙｕｓｅｄａｎｉｍａｌａｒｒｈｙｔｈｍｉｃｍｏｄｅｌｓｔｏｓｔｕｄｙｔｈｅｅｆｆｅ…     

Study on the Analgesic Effect and Mechanism of Zhitong Capsule (止痛胶囊) in Adjuvant Arthritis Rats

Clinical practice has proved that traditionalChinese medicine (TCM) has a preferable effect ofrelieving pain, and has been widely used in treat ment of patients with pain symptoms. Basing uponthis, we formulated a recipe Zhitong Capsule(止痛胶囊, ZTC) consisting of multiple ingredients ofChinese medicinal herbs ( CMH). Our previousstudy(1,2) had showed that ZTC had a preferableeffect of analgesia and anti inflammation. In orderto further study its mechanism of a…     

Study on effect of Yishen Capsule (益肾胶囊) in preventing recurrence of chronic glomerulonephritis

Objective:To observe the effect of Yishen Capsule (益肾胶囊,YSC) on preventing the recurrence of chronic glomerulonephritis (CGN) and to explore its mechanism preliminarily.Methods: CGN patients were assigned to the treated group (61 cases) and the control group (48 cases) and all of them were orally administered with 4 mg of Perindopril twice a day,but 3 capsules of YSC,thrice a day, were given additionally to patients in the treated group.The therapeutic course for both groups was 18 months.The recurrence rate of CGN at the 6th,12th,and 18th month in the two groups was observed and compared,and the changes of 24-h urinary protein quantity and T-lymphocyte subsets before and after treatment were observed as well.Results:(1) Comparison of recurrence rate between the two groups showed insignificant difference at the 6th month,but it did show significant difference at the 12th and the 18th month,which was significantly decreased in the treated group than in the control group (P<0.05,P<0.01);(2) The 24-h urinary protein quantity at the 18th month decreased significantly in both groups (P<0.05,P<0.01),but in the treated group was more significant (P<0.01);(3) T-lymphocyte subsets showed no obvious change in the control group after treatment (P>0.05),while in the treated group,it showed significant increase in CD3,CD4 and CD4/CD8 (P<0.05 or P<0.01) and significant decrease in CD8 (P<0.05),and also the difference after treatment in T-lymphocyte subsets between the two groups was significant (P<0.05 or P<0.01).Conclusion:YSC has marked effects in reducing the recurrence of CGN and in decreasing urinary protein,and its mechanism might be related with its function in regulating the ratio of T-lymphocyte subsets to enhance the immunity of patients.     

A Clinical Study on the Treatment of Chronic Pelvic Inflammation of Qi-stagnation with Blood Stasis Syndrome by Penyanqing Capsule (盆炎清胶囊)

Objective: To observe the clinical efficacy of Penyanqing Capsule (盆炎清胶囊, PYQC) in treating pelvic inflammation of Qi-stagnation with blood stasis syndrome. Methods: The randomized, single blinded, parallel positive drug controlled method was adopted, with 82 patients assigned into two groups by envelop method. The 42 patients in the treated group received PYQC 3 times a day, 4 capsules each time taken orally; the 40 patients in the control group were given orally Fuyankang tablets (妇炎康片, FYKT) 3 times a day, 6 tablets each time. The therapeutic course for both groups was 2 months, and 2 courses of treatment were given successively to observe the comprehensive effect, changes of symptoms and signs before and after treatment. The effects of PYQC on hemorrheological character in part of the patients and on the pathogenetic chlamydia and mycoplasma were also observed. Results: The total effective rate in the treated group was 83.3 %, which was insignificantly different from that in the control group ( 77.5 %, P ＞ 0.05 ).However, PYQC could significantly lower the hemorrheologic indexes in patients and showed definite influence on the pathogenetic chlamydia and mycoplasma. Conclusion: PYQC has good therapeutic effect in treating chronic pelvic inflammation of Qi-stagnation with blood stasis syndrome, and showed definite effect on chlamydia and mycoplasma.     

Clinical Study on Protective Effect of Xinmaitong Capsule(心脉通胶囊) on Damage of Vascular Endothelial Cells

Ｅｎｄｏｔｈｅｌｉｎ（ＥＴ）ａｎｄｃａｌｃｉｔｏｎｉｎｇｅｎｅｒｅｌａｔｅｄｐｅｐｔｉｄｅ（ＣＧＲＰ）ａｒｅａｐａｉｒｏｆｎｅｗｌｙｆｏｕｎｄｖａｓｃｕｌａｒａｃｔｉｖａｔｉｎｇｍｅｄｉａｗｉｔｈｏｐｐｏｓｉｔｉｖｅａｃｔｉｏｎ．ＥＴｉｓａｖａｓｏｃｏｎｓｔｒｉｃｔｉｖｅｐｅｐｔｉｄｅｐｒｏｄｕｃｅｄｂｙｖａｓｃｕｌａｒｅｎｄｏｔｈｅｌｉａｌｃｅｌｌｓ（ＶＥＣ）ｗｉｔｈｓｔｒｏｎｇａｎｄｐｒｏｔｒａｃｔｅｄｅｆｆｅｃｔ,ｔｈｅｓｔｒｏｎｇｅｓｔｖａｓｏｃｏｎｓｔｒｉｃｔｏｒｉｎｔｈｅｈｕｍａｎｂ…     

Effect of Xuefu Zhuyu Capsule (血府逐瘀胶囊) on Angiogenesis in Hindlimb Ischemic Rats

Objective: To investigate the effect and mechanism of Xuefu Zhuyu Capsule(血府逐瘀胶囊, XZC) on pro-angiogenesis in the hindlimb ischemic model rats. Methods: A total of 100 Sprague Dawley rats were randomly divided into a model group, a regular-dose XZC group(0.48 g·kg~(-1)·d~(-1)) and a high-dose XZC group(0.96 g·kg~(-1)·d~(-1)) using random number table method. The model of hindlimb ischemic rats were made through femoral artery embolization with Bletilla microsphere agent. XZC were given on the first day after embolization surgery and lasted 5 days. Finally 72 models were obtained with 12 in each group for each time point. The lower ischemic limb was amputated on the third day after embolization surgery. Histopathological characters and the number of blood vessels of granulation tissues were observed at 36 and 48 h after amputation, respectively. The main genes were obtained from microarray analysis and were validated using real-time quantitative polymerase chain reaction. Results: The vascular number of granulation tissues at both 36 and 48 h were characterized by new and fresh vessels. The number of angiogenesis in the high-dose XZC group at 36 and 48 h was greater compared with that in the regular-dose XZC and model groups(P0.01), and high-dose XZC at 36 h increased more vessels than that at 48 h(P0.01). Consequently, granulation tissues from the high-dose XZC group at 36 h were chosen for microarray analysis. In all, 2,085 differentially expressed genes(DEGs) were detected and 25 DEGs were determined to be directly related to angiogenesis. Four biological process terms were found including angiogenesis, regulation of angiogenesis, positive regulation of angiogenesis, and positive regulation of vascular endothelial growth factor receptor signaling pathway(P0.05). Microarray analysis also showed 49 pathways including 11 pathways related to angiogenesis. Conclusion: XZC promoted angiogenesis moderately and the mechanism involved multiple DEGs and multiple pathways.     

Experimental study on the anticancer effect of curcumin on mouse of S180 in vivo

Objective ：To study curcumin＇s growth inhibitory effects and morphology changes on sarcoma graft＇s of S180 mouse, with further inquiry into the possible mechanisms, Methods： Thirty S180 mouse were randomly assigned into 3 groups ： saline group （blank control group）, Cytoxan group （positive control group） and curcumin group. The tumor inhibitory rates, the index of thymus and spleen,the growth of tumor and the change of pathology-morph, the index of apoptosis cells and morphology changes of apoptosis cells in the different groups were observed. Results： （1） Tumor＇s inhibitory rate in curcumin group and cytoxan group was 68, 32% and 70. 43%, respectively. Compared to blank control group, the 2 groups had significant elevated tumor inhibitory rate （P〈0.01）. （2） Thymus index of curcumin group did not have significant decrease compared to blank control group （P〉0.05）, （3） Under electroscope,curcumin group and positive control group had significant decrease in terms of growth of tumor, degree of infiltration of tumor, the number of nucleus fission, and blood vessels number compared to saline group （P〈0.05）, However, the degree of cell necrosis, the number of splenic segments and macrophage are increased significantly compared to negative group, （4） Accumulative score of apoptosis cell in curcumin group was significantly higher than other two groups（P〈0.05）. Conclusion： Internal organ study and ceil morphology observation show curcumin can effectively inhibit the growth and cause the death of sarcoma graft of S180 mouse without interference with thymus.