sIL—2R及IgE在异位性皮炎患者血清的测定

为探讨异位性皮炎（ＡＤ）的发病机理,了解血清可溶性细胞介素２受体在ＡＤ患者血清中的意义,对４７例ＡＤ患者血清ｓＩＬ－２Ｒ及ＩｇＥ检测,并对１１例患者在治疗好转后予以复查。结果显示,ＡＤ患者的血清ＳＩＬ－２Ｒ显著高于正常对照组,但与ＡＤ的严重程度无显著关系。其中１１例患者经治疗后病情显著好转,而血清的ＳＩＬ－２Ｒ仍无显著下降。     

尖锐湿疣患者血清白介素－2及其可溶性受体的酶免疫检测

采用ＥＬＩＳＡ技术检测３０例ＣＡ患者血清ＩＬ－２水平，其中１８例患者同时作了血清可溶性ＩＬ－２受体（ｓＩＬ－２Ｒ）检测。结果发现ＣＡ患者血清ＩＬ－２水平比对照明显低下（Ｐ＜０．０５）、ｓＩＬ－２Ｒ则无明显改变，说明该病存在细胞免疫功能缺陷，此可能是ＣＡ发病的重要原因。     

尖锐湿疣患者血清白介素－2及其可溶性受体的酶免疫检测

采用ＥＬＩＳＡ技术检测３０例ＣＡ患者血清ＩＬ－２水平，其中１８例患者同时作了血清可溶性ＩＬ－２受体（ｓＩＬ－２Ｒ）检测。结果发现ＣＡ患者血清ＩＬ－２水平比对照明显低下（Ｐ＜０．０５）、ｓＩＬ－２Ｒ则无明显改变，说明该病存在细胞免疫功能缺陷，此可能是ＣＡ发病的重要原因。     

色素障碍性皮肤病

２００１１１３２白癜风患者外周血Ｔ淋巴细胞亚群和ｓＩＬ－２Ｒ水平变化的研究／汪新义（山东省皮防所）…／／临床皮肤科杂志．－２０００，２９（５）．－２５８～２６０ 采用 ＡＰＡＡＰ法和双抗体夹心 ＥＬＩＳＡ法，对１５０例自癜风患者外周血Ｔ淋巴细胞亚群和可溶性白细胞介素－２受体（ｓＩＬ－２Ｒ）进行了检测。结果显示寻常型白癜风ＣＤ３＋、ＣＤ４＋细胞数和ＣＤ４＋／ＣＤ８＋比值均显著低于正常对照（Ｐ＜０．０１），ｓＩＬ－２Ｒ活性明显高于节段型白癜风和正常人（Ｐ＜０．００１，Ｐ＜０．０１）；节段型自癜风ＣＤ３…     

尖锐湿疣患者血清白介素10水平及其与白介素2关系的研究

为了研究白介素（ＩＬ）１０在尖锐湿疣（ＣＡ）发病机理中的作用及其与ＩＬ２的关系，我们采用双抗体夹心ＥＬＩＳＡ技术检测２４例ＣＡ患者血清ＩＬ１０水平，其中１９例同时作了ＩＬ２水平测定。结果发现ＣＡ患者血清ＩＬ１０水平比正常人显著增高（Ｐ＜０．０５），ＩＬ２水平降低（Ｐ＜０．０５）；ＩＬ１０与ＩＬ２间呈负相关性（ｒ＝－０．８９４，Ｐ＜０．００１），说明ＩＬ１０对ＩＬ２产生有明显的抑制作用。本实验证明，ＣＡ患者存在着细胞因子产生失衡所引起的一系列细胞免疫反应抑制效应，在ＣＡ发病机理中起着重要作用。     

聚合酶链反应和直接免疫荧光法检测沙眼衣原体感染的方法比较

沙眼衣原体所致泌尿生殖道感染是最常见的性传播疾病之一。对１１０例患者进行聚合酶链反应（ＰＣＲ）与直接免疫荧光法（ＤＦＡ）检测，比较二者在临床检测中的优缺点。ＰＣＲ引物为主要外膜蛋白基因片段，阳性６５例（５９１％），阴性４５例（４０９％）。ＤＦＡ阳性４３例（３９１％），阴性６７例（６０９％），ＰＣＲ和ＤＦＡ阳性率有显著性差异（Ｐ＜００１），病程长短对ＰＣＲ阳性率无明显影响（Ｐ＞００５），病程长短对ＤＦＡ阳性率有显著影响（Ｐ＜００１），病程长者ＤＦＡ阳性率低。ＰＣＲ特别适用于临床中病程长且ＤＦＡ阴性患者。     

白癜风患者血清及皮肤组织液可溶性白介素2受体的检测

为了探讨可溶性白介素２受体（ｓＩＬ－２Ｒ）在白癜风发病中的作用，采用单克隆与多克隆双抗体夹心法，对４１例白癜风患者血清及白斑部位和无病变部位皮肤组织液中ｓＩＬ－２Ｒ水平进行了测定。结果显示：白癜风患者血清ｓＩＬ－２Ｒ水平明显高于正常对照组（Ｐ〈０．０１），其中寻常型与对照组相比有显著性差异（Ｐ〈０．０１），而节段型与对照组相比无显著性差异（Ｐ〉０．０５）；进展期白癜风患者血清中ｓＩＬ－２Ｒ水平明显     

性激素与尖锐湿疣人乳头瘤病毒的相关性研究

为了探讨尖锐湿疣人乳头瘤病毒感染与性激素的关系，采用斑点杂交、免疫组化和放免测定等技术，检测了重庆地区４７例女性尖锐湿疣（ＣＡ）患者病变组织中ＨＰＶＤＮＡ、雌激素受体（ＥＲ）以及血清中雌二醇（Ｅ２）、孕酮（Ｐ）和睾酮（Ｔ）。结果提示：妊娠期ＨＰＶＤＮＡ含量、ＥＲ表达量均明显高于对照组和其余各组（Ｐ＜０．００１），健康组中无ＥＲ表达；ＨＰＶＤＮＡ含量与ＥＲ表达量之间呈正相关（Ｐ＜０．００１），与血清Ｅ２、Ｐ也呈正相关（Ｐ＜０．００１），与Ｔ无明显相关（Ｐ＞０．０５）。我们认为性激素特别是雌激素、孕激素在ＨＰＶ感染中可能有协同作用     

白癜风患者的可溶性白细胞介素2受体研究

检测１６例白癜风患者的ｓＩＬ－２Ｒ水平，结果表明；１６例患者ｓＩＬ－２Ｒ水平明显高于正常人（Ｐ＜０．００１）。这一结果说明白癜风与免疫异常有密切关系。     

系统性红斑狼疮患者外周血单一核细胞CD23的表达

为了揭示Ｂ细胞中ＣＤ２３的表达与ＳＬＥ发生发展的关系及在ＳＬＥ发病机理中可能的作用，我们应用ＡＢＣ免疫组化法和斑点核酸杂交技术对ＳＬＥ患者外周血单一核细胞（ＰＢＭＣ）ＣＤ２３蛋白和ｍＲＮＡ表达进行了检测。结果显示：３０例ＳＬＥ患者ＰＢＭＣＣＤ２３蛋白表达显著增高（Ｐ＜０．０１），且与疾病活动呈正相关关系（ｒｓ＝０．３８１４，Ｐ＜０．０５）；具有不同ＡＮＡ、抗ｄｓＤＮＡ抗体水平，有无伴肾损、脑损的ＳＬＥ患者，ＰＢＭＣＣＤ２３表达均无显著性差异（Ｐ均＞０．０５）；单纯使用皮质类固醇激素治疗或和其它免疫抑制剂联合治疗的ＳＬＥ患者，ＰＢＭＣＣＤ２３表达亦无显著性差异（Ｐ＞０．０５）。２０例ＳＬＥ患者ＰＢＭＣＣＤ２３ｍＲＮＡ表达较正常人显著增高（Ｐ＜０．０１）。经治疗病情稳定后，ＣＤ２３蛋白和ｍＲＮＡ表达均降至正常（Ｐ均＞０．０５）。提示在ＳＬＥ活动期Ｂ细胞高度激活、增殖并大量表达ＣＤ２３，且该种表达与ＡＮＡ、抗ｄｓＤＮＡ抗体产生水平无直接关系     

银屑病患者血清及皮损组织液sIL-2R检测及其临床意义

目的：探讨血清及皮损组织液可溶性白细胞介素２受体水平与银屑病活动和型别的关系。方法：采用双抗体夹心法测定不同类型银屑病患者治疗前后血清及皮损组织液ｓＩＬ－２Ｒ水平。结果;寻常型,红皮病型,脓疱型和关节型银屑病患者血清ｓＩＬ－２Ｒ水平在进展期均高于正常对照组,皮损消退后,则明显下降;     

银屑病患者光量子治疗前后血清中IL—2及sIL—2R含量的变化

为进一步探索紫外线照射自血回输治疗寻常型银屑病的机理，应用ＴＵＶＩＳＢ对２４例ＰＶ患者进行治疗，并对治疗前后患者血清中白细胞介素－２及可溶性白细胞介素２受体水平进行检测。     

Factors associated with the relapse of infantile haemangiomas in children treated with oral propranolol

Background Hidradenitis suppurativa (HS) is a chronic inflammatory and debilitating disease of the skin. No biomarkers for this disease exist. Objectives We set out to test whether angiotensin‐converting enzyme (ACE), lysozyme, soluble interleukin 2 receptor (sIL‐2R) and S100A8/A9 (calprotectin) are elevated in patients with HS. Methods Serum was collected from 29 patients with HS at different stages of the disease, and from 51 controls. ACE, lysozyme, sIL‐2R and S100A8/A9 levels were measured. Clinical observation of disease activity was scored according to the Hurley grading system and by a physician global score (PGS) of disease severity. Results Serum levels of lysozyme and ACE were not increased above the normal reference values in controls or patients with HS. Levels of sIL‐2R and S100A8/A9 were significantly higher in patients with HS than in controls (P < 0·001 for both sIL‐2R and S100A8/A9). Based on the receiver operating characteristic curves, the optimum sIL‐2R and S100A8/A9 cut‐off values were 375 U mL^?1 and 680 ng mL^?1, respectively, with a sensitivity of 0·79 and specificity of 0·78 for sIL‐2R, and 0·86 and 0·88, respectively, for S100A8/A9. No correlations with Hurley classification scores were found. However, when using PGS of disease activity to categorize patients, levels of S100A8/A9, but not sIL‐2R, tended to be higher in patients with more active disease. Conclusions Levels of S100A8/A9 and sIL‐2R, but not ACE or lysozyme, are elevated in the serum of patients with HS. However, there is no correlation between S100A8/A9 or sIL‐2R levels and disease stage according to the Hurley classification system. Further research is needed to study the potential of S100A8/A9 to score disease activity in larger cohorts of patients and to predict disease flares.     

银屑病患者血清sE-selectin、slCAM-1、sIL-2R水平的检测

目的：探讨血清中可溶性的Ｅ－选择素,细胞间粘附分子１（ｓＩＣＡＭ－１）及白介素－２受体ｓＩＬ－２Ｒ水平与银屑病的关系及其人临床意义。方法：采用ＥＬＩＳＡ法,测定３１例斑块状银屑病患者治疗前,治疗后（３０例）及２０例健康人血清中ｓＥ－ｓｅｌｅｃｔｉｎ,ｓＩＣＡＭ－２Ｒ水平。结果：银屑病患者治疗前血清ｓＥｌ－ｓｅｌｅｃｔｉｎ,ｓＩＣＡＭ－１,ｓＩＬ－２Ｒ明显增高,与对照组比较有显著差异,其中ｓＥ－ｓｅ     

特异性免疫治疗对特应性皮炎患者血清IL-4、IFN-γ的影响

目的：探讨Th1／Th2细胞因子在特应性皮炎（AD）发病机制中的作用,以及特异性免疫治疗（specific immunotherapy,SIT）对AD患者血清IL-4、IFN-γ的影响。方法：采用双抗体夹心ELISA法检测SIT治疗前后AD患者白介素4（IL-4）、干扰素γ（IFN-γ）水平的变化。结果：AD患者IL-4、IFN—γ水平高于正常对照组（P〈0．01）;经SIT治疗后,AD患者IFN-γ水平升高（P〈0．05）,IL-4水平明显下降（P〈0．01）。结论：AD存在Th1／Th2细胞分化失衡;SIT可明显调节患者细胞因子水平。     

肌炎患者皮质类固醇治疗前后sIL-2R、 IL-6水平的研究

目的 探讨可溶性白介素 2受体（ sIL 2R）及白介素 6（ sIL 6）与皮肌炎发病机理的关系,分析 sIL 2R与血清肌酶的关系。 方法 用双抗体夹心 ELISA法及 3H－胸腺嘧啶核苷掺入法检测了 30例皮肌炎患者用皮质类固醇治疗前后及 28例正常人 sIL 2R、 IL 6水平。结果 皮肌炎患者治疗前 sIL 2R、 IL 6显著升高（ P0.05） ,IL 6仍高于正常（ P0.05）。结论 sIL 2R、 IL 6在皮肌炎的发病机理中起着重要作用, sIL 2R水平可能与皮肌炎的活动性相关, sIL 2R、 IL 6对于皮肌炎合并肿瘤的患者的意义尚需进一步研究。     

Serum levels of IL‐18 and sIL‐2R in patients with alopecia areata receiving combined therapy with oral cyclosporine and steroids

Please cite this paper as: Serum levels of IL‐18 and sIL‐2R in patients with alopecia areata receiving combined therapy with oral cyclosporine and steroids. Experimental Dermatology 2010; 19: 145–147. Abstract: This study was to determine which immunologic factors contribute to the prognosis of patients with alopecia areata (AA) who were receiving oral cyclosporine A and methylprednisolone. Patients with >25% hair regrowth were defined as responders, and patients exhibiting ≤25% regrowth were poor‐responders. The serum levels of IL‐18 and soluble IL‐2 receptor (sIL‐2R) were measured at baseline in 21 patients with AA and 22 control subjects. The mean serum level of IL‐18 in the patients with extensive AA was significantly higher than that in the control subjects. The mean serum concentration of sIL‐2R in the AA patients significantly decreased after 1 month of treatment. The mean basal serum level of IL‐18 was highest in the responder, whereas the baseline level of sIL‐2R was significantly higher in the poor‐responder group than other groups. In conclusion, increased serum sIL‐2R level and lower IL‐18 level at baseline was associated with a poor prognosis in patients with AA.     

CC chemokines as potential immunologic markers correlated with clinical improvement of atopic dermatitis patients by immunotherapy

Please cite this paper as: CC chemokines as potential immunologic markers correlated with clinical improvement of atopic dermatitis patients by immunotherapy. Experimental Dermatology 2010; 19: 246–251. Abstract: Although immunotherapy is not accepted as a curative treatment for atopic dermatitis (AD), most studies have shown positive effects of immunotherapy on AD patients. The serum levels of CC chemokine ligand 17 (CCL17), CCL22 and CCL18 have been reported to be highly correlated with disease severity, which suggests important roles for CC chemokines in the pathogenesis of AD. Objective: The purpose of this study was to investigate the changes in clinical and immunologic markers before and after immunotherapy and to find which CC chemokines correlate with clinical improvement after immunotherapy with house dust mite (HDM) allergens in AD patients. Methods and Results: A total of 20 AD patients who were sensitized to HDM allergens through a skin‐prick test and Pharmacia CAP system were treated with subcutaneous immunotherapy using HDM allergens (treatment duration 12–60 months). Eczema area and severity index scores in 20 patients with AD decreased significantly after immunotherapy (P < 0.001). Serum total immunoglobulin E (IgE) and Dermatophagoides pteronyssinus‐specific IgE levels tended to decrease after treatment although this was not statistically significant, and the D. farinae‐specific IgE level showed no change. Serum CCL17, CCL22 and CCL18 levels decreased significantly from baseline after treatment (P = 0.043, 0.017 and <0.001, respectively). The percentage reductions in serum CCL17 and CCL22 level were significantly correlated with reductions in disease severity (P = 0.007, R² = 0.301 and P = 0.037, R² = 0.177, respectively). Conclusion: We suggest that CCL17 and CCL22 are good immunological marker candidates that can be used to assess clinical improvement after immunotherapy in AD patients.     

Reduction of serum interleukin-5 levels reflect clinical improvement in patients with atopic dermatitis.

Cytokines, in particular IL-4 and IL-5, regulate IgE synthesis and eosinophil activation in atopic dermatitis (AD). To elucidate whether the serum levels of IL-4 and IL-5 are related to the serum IgE level, eosinophilia, or clinical severity of the disease, 25 cases with AD were studied. Blood samples were isolated from two groups of donors: 1) patients with AD (n = 25); 2) non-allergic individuals (NA, n = 20) with serum IgE levels below 100 IU/ml and with blood eosinophil counts below 250/microliter. Each parameter was evaluated at least twice in AD patients at the beginning of the study and after 4, 8 or 12 weeks of treatment. IL-4 was hardly detected in AD and NA, but IL-5 was increased (> 10 pg/ml) in most cases (22/25) of AD group with 513.6 pg/ml as the mean. AD with normal serum IgE levels exhibited increased levels of IL-5, whereas AD with high serum IgE levels did not necessarily have elevated IL-5 levels. The IL-5 level tended to change in parallel with the clinical severity in each AD case, although the level itself was not correlated with the clinical severity per se. A significant decrease of IL-5 was observed in AD when the clinical severity decreased. Eosinophils also decreased along with the improvement of AD, whereas the serum level of IgE did not change during the observation period. Our results suggest that IL-5 is involved in the regulation of clinical courses of AD and that its kinetics at the serum level reflects the clinical activity of AD.     

STUDY OF THE ROLE OF SERUM FOLIC ACID IN ATOPIC DERMATITIS: A CORRELATION WITH SERUM IgE AND DISEASE SEVERITY

Background:

Most atopic dermatitis (AD) patients have elevated serum immunoglobulin E (IgE). Impaired folic acid (FA) metabolism was found to reduce the intracellular methyl donor pool, associated with a higher prevalence of atopy.

Aim:

To assess serum IgE and FA in AD patients and to correlate their levels with the disease severity, and with each other.

Materials and Methods:

Twenty patients with AD were assessed for serum FA and IgE, compared with 20 age- and sex-matched controls. Patients were classified into three groups (mild, moderate, and severe AD) based on clinical severity according to Nottingham index. In both patients and controls, serum IgE was measured using Enzyme-linked immunosorbent assay technique and serum FA was measured using Microparticle Enzyme Immunoassay technique.

Results:

Serum FA levels were lower in AD patients compared with controls, but the difference was not statistically significant. FA levels did not show statistically significant difference among disease severity groups and did not correlate with serum IgE levels. On the other hand, serum IgE levels were significantly elevated in AD patients compared with controls, and among AD patients, its levels were significantly elevated in severe AD compared with mild and moderate disease.

Conclusion:

Serum IgE is useful in assessment of AD severity and activity. FA contribution to AD needs further investigations.