Effect of Electro-acupuncture on Rat Joint Pathomorpnology of Chronic Adjuvant Arthritis Model

Objective:To study the effect of electro-acupuncture (EA) on pathomorphological changes of joints in rat model of chronic adjuvant arthritis. Methods: The rat chronic adjuvant arthritis model was established by subcutaneous injection of 0.1 ml of complete Freund's adjuvant to the left hind sole. Forty Wistar rats were randomly divided into the model group, the low frequency (2 Hz) EA group, the high frequency EA (100 Hz) group and the body acupuncture group. After being modeled except the model group, the other three groups were treated with EA or body acupuncture in Yanglingquan points (bilateral) for 3 weeks, the left ankle joints and metatarsal joints of rats were taken for pathological examination by fixing with 10% formalin and embedding in paraffin, sectioning and staining with HE. Results: Obvious inflammatory cell infiltration, loosened synovial tissue, damage of articular cartilage and proliferation of synovial cells and granulation tissue were observed in the sections of joints in model rats.     

Effect of Triptolide on Expression of Receptor Activator of Nuclear Factor-κB Ligand in Rat Adjuvant Induced Arthritis

Receptor activator of nuclear factor-kappaBligand(RANKL)promotes osteoclast formation,fusion,differentiation and activation.It plays akey role in osteoclast mediated bone erosion inrheumatoid arthritis(RA).Osteoprotegerin(OPG),as a soluble decoy receptor of RANKL,can prevent bone erosion in RA.Our previousstudies have shownthat Triptolide(TP),an activecompoundidentifiedin a traditional Chinese herb--Tripterygium wilfordii Hook F(TWHF),can ef-fectively inhibit bone destruction in ex…     

Effect of Tongbiling (通痹灵) on the Synoviocyte Function in Adjuvant Arthritis Rats

Ｒｈｅｕｍａｔｏｉｄａｒｔｈｒｉｔｉｓ（ＲＡ）ｉｓａｃｈｒｏｎｉｃｉｍｍｕｎｅ ｍｅｄｉａｔｅｄｉｎｆｌａｍｍａｔｏｒｙｄｉｓｅａｓｅ．ＴｈｅｅａｒｌｙｌｅｓｉｏｎｏｆＲＡｍａｉｎｌｙｌｏｃａｔｅｄａｔｓｙｎｏｖｉａｌｍｅｍｂｒａｎｅ．Ｖａｒｉｏｕｓｃｙｔ     

Detrimental Effect of Free Radicals on Tissues in Rat with Burn Shock

A model of burn shock was reproduced in rats and the changes of water content andmalondialdehyde(MDA)concentration in tissues were measured.The results showed that there wasa significant increase of MDA concentration and water content in lung and bumed skin post burnand a positive correlation was found between MDA level and water content.Pretreatment withsuperoxide dismutase(SOD)prevented effectively lung and skin from edema.The role of oxygen freeradicals in vascular permeability disturbance and development of edema in burns was discussed.     

The Effect of Tripterygium Wilfordii Monomer T4 on Rat Spermatid Nuclear Protein Transition

（费仁仁）（陈晖）（戴文平）（刘平）（陈啸梅）（薛社普）ＴｈｅＥｆｆｅｃｔｏｆＴｒｉｐｔｅｒｙｇｉｕｍＷｉｌｆｏｒｄｉｉＭｏｎｏｍｅｒＴ４ｏｎＲａｔＳｐｅｒｍａｔｉｄＮｕｃｌｅａｒＰｒｏｔｅｉｎＴｒａｎｓｉｔｉｏｎ￥ＦｅｉＲｅｎ－ｒｅｎ；ＣｈｅｎＨｕ...     

Effect of Electroacupuncture on Reperfusion Ventricular Arrhythmia in Rat

Clinically,heartthrombolytictreatmentandopen heartsurgerypossiblygiverisetosevereven triculararrhythmia,evenlethalarrhythmia.Therefore,thesearchforpreventingtheheartfromreperfusion inducedmalignantarrhythmiashasbeenatopicofimmenseinterest.Thereisalargebodyofevidencesupportingthatelectroacu puncture(EA)at"Neiguan"acupointhasastrongprotectiveeffectonischemicmyocardium[1].OurpreviousstudyfoundthatEAatthe"Neiguan"and"Lingdao"acupointsincreasedthethresholdofventricularfibrillationinratswithacute…     

Effect of Selenium on Aflatoxin Hepatocarcinogenesis in the Rat

The effects of selenium (Na_2SeO_3) on aflatoxin B_1 (AFB_1)-induced hepatic neoplasia were studied in the rat. Putative preneoplastic foci and nodules composed of basophilic, eosinophilic, and clear cells developed early. Basophilic foci were seen first; in the later stages basophilic and eosinophilic nodules predominated. At each stage the AFB_1 + Se groups showed fewer and smaller foci and nodules than the AFB_1 -Se group. The number of foci in the AFB_1 + 3 ppm Se group and their mean area were smaller than those in the 6 ppm Se + AFB_1 group. At the end of the experiment hepatocellular carcinoma (HCC) was found in 11/18 rats (61%) of the AFB_1-Se group. HCC was not found in either of the groups given AFB_1 +Se. We conclude that Se had an inhibitory effect on the initiation and promotion stages of AFB_1-induced preneoplastic foci and nodules. Se also prevented progression of these nodules to HCC even after cessation of AFB_1 administration. The inhibitory effect of Se at 3 ppm was greater than at 6 ppm. The 6 ppm Se group also showed evidence of toxicity. 1990 Academic Press.Inc.     

Study on the Analgesic Effect and Mechanism of Zhitong Capsule (止痛胶囊) in Adjuvant Arthritis Rats

Clinical practice has proved that traditionalChinese medicine (TCM) has a preferable effect ofrelieving pain, and has been widely used in treat ment of patients with pain symptoms. Basing uponthis, we formulated a recipe Zhitong Capsule(止痛胶囊, ZTC) consisting of multiple ingredients ofChinese medicinal herbs ( CMH). Our previousstudy(1,2) had showed that ZTC had a preferableeffect of analgesia and anti inflammation. In orderto further study its mechanism of a…     

Clinical Observation on Treatment of Senile Rheumatoid Arthritis by Tripterygium Polyglycosides Combined with Small Dose of Methotrexate

Objective: To observe the effect and adverse reaction of tripterygium polyglycosides (TP) combined with small dose of methotrexate (MTX) in treating senile rheumatoid arthritis (RA). Methods: Sixty old patients with RA were equally divided into two groups, the treated group was treated with oral intake of TP (10 mg three times per day) plus MTX (7.5 mg, once per week), and the control group was treated with MTX (15 mg per week) respectively for 3 months. The changes from before to after treatment in resting pain, morning rigidity, bilateral grip strength, local tenderness index, arthroncus index, patient's evaluation, doctor's evaluation, erythrocyte sedimentation rate (ESR), C-reaction protein (CRP) and rheumatoid factor(RF) titre were observed and the clinical therapeutic effect assessed. Results: The total effective rate in the treated group was 86.67% and that in the control group 83.33% , showing insignificant difference between them (P>0.05). But the improvement in local tenderness index, arthroncu     

Clinical Study on Tripterygium Wilfordii Complex Ester Tablet in Treating Rheumatoid Arthritis

Clinical Study on Tripterygium Wilfordii Complex Ester Tablet in Treating Rheumatoid Arthritis@杜秀兰@张宏@傅新利@张之澧@张梅涧@马永祯     

樱桃花青素苷对佐剂性关节炎大鼠免疫功能和炎症因子的影响

目的观察樱桃花青素苷对佐剂性关节炎大鼠T淋巴细胞亚群、脾细胞增殖及血清IL-6和足爪PGE2水平的影响。方法将60只SD雄性大鼠随机分为6组,即正常组、模型组、樱桃花青素苷高、中、低剂量(300、150、75mg/kg)组及雷公藤多苷(8.75mg/kg)治疗组(简称雷公藤组),除正常组外,用弗氏完全佐剂制备模型。流式细胞术检测大鼠外周全血中CD3、CD4、CD8T细胞亚群和CD4/CD8值;光镜下观察踝关节组织形态学改变;MTT法检测脾细胞增殖能力;放免法检测血清IL-6水平与足爪匀浆中PGE2水平。结果与正常组相比,模型组外周全血中CD3、CD4T细胞下降(P<0.01),樱桃花青素苷和雷公藤组外周全血中CD3、CD4T细胞升高。光镜下组织形态观察表明花青素苷高剂量组和雷公藤组能减轻滑膜增生,减少炎性细胞浸润,减轻组织损伤。在ConA刺激下模型组大鼠脾细胞增殖反应与正常组相比降低,樱桃花青素苷高剂量能使模型大鼠低下的脾细胞增殖反应恢复至接近正常。模型组血清IL-6水平高于正常组,樱桃花青素苷各剂量组血清IL-6明显降低(P<0.01);模型组足爪PGE2水平高于正常组(P<0.01),樱桃花青素苷高剂量组和雷公藤组PGE2水平比模型组降低(P<0.01)。结论樱桃花青素苷高剂量对佐剂性关节炎大鼠模型的T淋巴细胞亚群和免疫功能有调节作用,并能显著降低炎症细胞因子IL-6和足爪PGE2水平,从而减轻模型大鼠的关节炎损伤。     

Effect of tripterygium glycosides on pulmonary function in adjuvant arthritis rats

BackgroundTripterygium is a Chinese herb with immunosuppressive effects and an established history of use in the treatment of rheumatoid arthritis. Previous studies demonstrated that tripterygium glycosides (TPG) alleviated Freund’s complete adjuvant (FCA)-induced arthritis. Simultaneously, it has also been observed to impact the adjuvant arthritis (AA) associated with lung injury. In this study, we have investigated whether traditional Chinese medicine could attenuate lung injury induced by AA by observing the effects of TPG on the degree swelling, arthritis index (AI), lung index (LI), pulmonary function, cytokines, and the expression of regulatory T cells (Treg) and Foxp3 in AA rats.MethodsA total of 48 rats were separated into four groups: normal control (NC), model control (MC), methotrexate (MTX), and TPG groups (12 in each). Except for the rats of NC group, those in the others groups were intracutaneously injected in the right hind limb with 0.1 ml of FCA. The NC and MC groups were treated with physiological saline, and the MTX and TPG groups were treated with MTX and TPG, respectively. Thirty days after administration, the changes in swelling degree, AI, LI, pulmonary function, Treg levels, the ultrastructure of the lung tissue, and the expression of Foxp3 in the lung tissue were observed.ResultsCompared with NC group, the level of swelling degree, AI, LI, 1 second average expiratory flow (FEV₁/FVC %), the alveolar inflammation integration, tumor necrosis factor alpha (TNF-α), and endothelium-1 (ET-1) in the MC group had significantly increased (p < 0.01). However, the level of forced vital capacity (FVC), 25% vital capacity of the peak expiratory flow (FEF₂₅), 50% vital capacity of the peak expiratory flow (FEF₅₀), 75% vital capacity of the peak expiratory flow (FEF₇₅), maximum mid-expiratory flow (MMF), peak expiratory flow (PEF), interleukin-10 (IL-10), CD4⁺ CD25⁺ Treg, and Foxp3 had significantly decreased (p < 0.01). LI, the alveolitis score, and ET-1 were found to decrease with TPG treatment. However, the levels of FVC, FEF₂₅, FEF₅₀, FEF₇₅, MMF, PEF, IL-10 in serum, and CD4⁺ CD25⁺ Treg in peripheral blood had increased. The expressions of Foxp3 protein and mRNA in the lung tissue had also increased in the TPG group. Compared with the MTX group, the pulmonary function had enhanced, the structure of alveolar type II cells had improved, and the expression of the IL-10, Treg, and Foxp3 had elevated. However, the TNF-α and ET-1 levels had reduced as compared to the MTX group.ConclusionThe level of paw swelling and AI in the AA rats can be inhibited by TPG. The inflammatory response in lung tissue had also decreased, although there was significant improvement in the pulmonary function. The mechanism that would explain this observation is probably associated with the upregulation of the expression of IL-10, Treg, and Foxp3 and downregulation of the expression of TNF-α and ET-1.     

豨莶醇对佐剂性关节炎大鼠血清细胞因子及滑膜细胞凋亡蛋白的影响

目的探讨豨醇(奇任醇)对佐剂性关节炎(AA)大鼠血清细胞因子白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)及滑膜细胞Bcl-2、Fas-L蛋白表达的影响。方法采用弗氏完全佐剂建立SD大鼠AA模型,分别给予奇任醇、氢化可的松、阿司匹林 环磷酰胺治疗;ILISA法测定实验大鼠血清IL-1β、TNF-α、IL-6水平;踝关节滑膜细胞进行Bcl-2、Fas-L免疫组化染色,运用计算机图像分析系统对染色结果进行分析。结果与正常组相比,模型组大鼠血清IL-1β、TNF-α、IL-6水平显著升高(P<0.01),滑膜细胞Bcl-2蛋白表达显著升高(P<0.05),Fas-L表达显著降低(P<0.05);与模型组比较,奇任醇组大鼠血清IL-1β、TNF-α、IL-6水平显著降低(P<0.01、0.05);滑膜细胞Bcl-2蛋白表达显著下调(P<0.05),Fas-L蛋白水平显著升高(P<0.05)。结论奇任醇可降低AA大鼠血清细胞因子IL-1β、TNF-α、IL-6水平,下调滑膜细胞Bcl-2蛋白表达、促进Fas-L蛋白表达。     

雷公藤多甙对佐剂性关节炎大鼠自由基和炎症因子的影响

目的探讨雷公藤多甙对佐剂性关节炎(AA)大鼠的保护作用及可能机制.方法采用大鼠AA模型,将30只SD雄性大鼠随机分为3组即正常组、模型组、雷公藤多甙组.对足爪肿胀进行测量,在光镜下观察各组组织形态学变化,生化方法检测了动物血清超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量以及总抗氧化能力(T-AOC).用放射免疫法检测了足爪TNF-α及 PGE2含量.用免疫组织化学方法检测了膝关节软骨环氧化酶-2(COX-2)及核因子κB(NF-κB)表达.结果与正常组相比,模型组血清SOD和T-AOC能力下降(P<0.01),MDA含量升高;足爪TNF-α及PGE2含量升高;膝关节软骨COX-2及NF-κB表达增加.雷公藤多甙组能升高血清SOD和T-AOC活力,降低血清MDA、足爪TNF-α、PGE2含量及膝关节软骨COX-2及NF-κB表达.光镜下组织形态观察表明雷公藤多甙组能减轻滑膜增生,减少炎细胞浸润.结论雷公藤多甙对AA模型具有保护作用,抗氧化能力及抑制炎症细胞因子浸润可能是其主要的作用机制之一.     

山茱萸鞣质活性部位对佐剂性关节炎大鼠免疫功能的影响

目的 研究山茱萸免疫抑制活性部位(鞣质活性部位，F-1C)对佐剂性关节炎(AA)大鼠免疫功能的影响。方法 运用淋巴细胞增殖反应、流式细胞技术和酶联免疫吸附试验(ELIsA)观察F-1C体内和体外对AA大鼠免疫功能的影响，并与环孢素A(CsA)和雷公藤多苷片(Tw)进行比较。结果 ig给予F1C(30mg／kg)对AA大鼠原发性足肿胀具有明显的治疗作用，该作用较TW强，较CsA弱；F-1C对AA大鼠低下的脾细胞增殖反应具有改善作用；对亢进的胸腺细胞增殖反应具有抑制作用。F-1C体外对AA大鼠低下的脾细胞产生IgG水平具有明显的促进作用，并能抑制亢进的胸腺细胞增殖反应。结论 F-1C能够治疗AA大鼠原发足肿胀，该作用可能与纠正AA大鼠的异常的免疫反应有关。     

Effect of electro-acupuncture on rat joint pathomorphology of chronic adjuvant arthritis model

Objective:To study the effect of electro-acupuncture (EA) on pathomorphological changes of joints in rat model of chronic adjuvant arthritis.Methods: The rat chronic adjuvant arthritis model was established by subcutaneous injection of 0.1 ml of complete Freund’s adjuvant to the left hind sole. Forty Wistar rats were randomly divided into the model group, the low frequency (2 Hz) EA group, the high frequency EA (100 Hz) group and the body acupuncture group. After being modeled except the model group, the other three groups were treated with EA or body acupuncture in Yanglingquan points (bilateral) for 3 weeks, the left ankle joints and metatarsal joints of rats were taken for pathological examination by fixing with 10% formalin and embedding in paraffin, sectioning and staining with HE.Results: Obvious inflammatory cell infiltration, loosened synovial tissue, damage of articular cartilage and proliferation of synovial cells and granulation tissue were observed in the sections of joints in model rats. These pathological changes were significantly improved after treatment, and the effect in the high frequency EA group were significantly superior to that in the low frequency EA and body acupuncture group.Conclusion: High frequency EA could significantly improve the pathomorphological changes of joints in chronic adjuvant arthritis rat models.     

白饭树提取物急性毒性实验及抗佐剂性关节炎研究

目的:研究白饭树提取物急性毒性实验及抗小鼠原发性佐剂性关节炎.方法:白饭树不同提取物以最大浓度对实验组小鼠灌胃给药,对照组给予等体积的生理盐水,连续观察14 d,记录小鼠毒副反应情况;灌胃白饭树不同提取物,1h后注射Freund's完全佐剂使小鼠右脚肿胀,观察白饭树不同提取物对佐剂性关节炎原发性足肿胀在18 h内的变化...     

雷公藤多甙对糖皮质激素受体的影响

目的 :研究雷公藤多甙对大鼠脾细胞GCR的影响。方法 :用雷公藤多甙处理ADI和ADX大鼠以及体外培养的大鼠脾细胞 ,检测大鼠脾细胞GCR、cAMP、对PHA的增殖反应和血浆CS。结果 :雷公藤多甙能升高脾细胞GCR数量、cAMP浓度、降低PHA的增殖反应和升高血浆CS浓度。结论 :雷公藤多甙降低大鼠脾细胞对PHA的增殖反应可能与升高GCR有关。     

玉津酒治疗大鼠佐剂性关节炎及免疫学的观察

目的:探讨玉津药酒对大鼠佐剂性关节炎(AA)治疗效果及免疫学改变.方法:以完全弗氏佐剂诱导大鼠AA模型,灌胃服用玉津酒,观察AA大鼠病症改善情况及对免疫功能的影响.结果:在诱导AA模型前灌胃服用玉津酒,可推迟大鼠AA的发病时间,降低发病率,减轻关节炎症状;在大鼠发生AA后服用玉津酒,可明显抑制关节炎症程度;服用玉津酒可抑制AA大鼠T、B淋巴细胞增殖反应及IL-1活性.结论:玉津酒是通过机体免疫调节达到对AA的预防和治疗作用.