Two new homoisoflavonoids from the fibrous roots of Ophiopogon japonicus (Thunb.) Ker-Gawl

Two new homoisoflavonoids ophiopogonone D (1) and ophiopogonanone G (2) were isolated from the fibrous roots of Ophiopogon japonicus. The structures of these two compounds were determined on the basis of spectroscopic means including HR-ESI-MS, 1D, and 2D NMR experiments. The cytotoxic activities of 1 and 2 against Hela and Hep2 cells are described.     

Two new furostanol glycosides from the fibrous root of Ophiopogon japonicus (Thunb.) Ker-Gawl

Two new furostanol glycosides, ophiopogonins H (1) and I (2), were isolated from the fibrous root of Ophiopogon japonicus. The structures of 1 and 2 were established as (25R)-26-[(O-β-d-glucopyranosyl-(1 → 2)-β-d-glucopyranosyl)]-22α-hydroxyfurost-5-ene-3-O-[α-l-rhamnopyranosyl-(1 → 2)]-β-d-glucopyranoside and (25R)-26-[(O-β-d-glucopyranosyl-(1 → 2)-β-d-glucopyranosyl)]-20α-hydroxyfurost-5,22-diene-3-O-[α-l-rhamnopyranosyl-(1 → 2)]-β-d-glucopyranoside on the basis of spectroscopic means including HR-ESI-MS, 1D and 2D NMR experiments.     

Two novel furostanol saponins from Ophiopogon japonicus

Two novel furostanol saponins were isolated from the fresh tubers of Ophiopogon japonicus. Comprehensive spectroscopic analysis allowed the chemical structures of the compounds to be assigned as (25R)-26-[(O-β-d-glucopyranosyl-(1 → 2)-β-d-glucopyranosyl)]-22α-hydroxyfurost-5-ene-3-O-β-d-xylopyranosyl-(1 → 4)-O-[α-l-rhamnopyranosyl-(1 → 2)]-β-d-glucopyranoside (1, ophiopogonin F) and (25R)-26-[(O-β-d-glucopyranosyl-(1 → 6)-β-d-glucopyranosyl)]-22α-hydroxyfurost-5-ene-3-O-β-d-xylopyranosyl-(1 → 4)-O-[α-l-rhamnopyranosyl-(1 → 2)]-β-d-glucopyranoside (2, ophiopogonin G). The rare furostanol saponins with two glucosyl residues at C-26 position were isolated from the natural source for the first time.     

A new C21 steroidal glycoside from Cynanchum inamoenum (Maxim.) Loes

A new C21 steroidal glycoside, 5beta,6beta-epoxy-glaucogenin C-3-O-beta-D-glucopyranosyl-(1 --> 4)-beta-d-glucopyranosyl-(1 --> 4)-beta-D-cymaropyranosyl-(1 --> 4)-beta-D-oleandropyranosyl-(1 --> 4)-beta-D-oleandropyranoside, named inamoside D (1), was isolated from the MeOH extract of the roots of Cynanchum inamoenum (Maxim.) Loes (Asclepiadaceae). In addition, five known compounds, including 7-demethoxytylophorine (2), dehydrodiconiferyl alcohol gamma'-O-beta-D-glucopyranoside (3), beta-d-fructofuranosyl-(2 --> 1)-alpha-D-(6-O-sinapoyl)-glucopyranoside (4), neohancoside C (5), and cuchiloside (6), have also been isolated. The structure of 1 was determined by 1D- and 2D-NMR spectroscopies. All the compounds were isolated from this plant for the first time.     

New sesquiterpenoid glycoside and phenylpropanoid glycosides from the tuber of Ophiopogon japonicus

A new sesquiterpenoid glycoside, cryptomeridiol 11-O-β-d-xylopyranosyl-(1→6)-β-d- glucopyranoside (1), two new phenylpropanoid glycosides, 3,4-dihydroxy-allylbenzene 3-O-β-d-glucopyranosyl-4-O-β-d-apiofuranosyl-(1→6)-β-d-glucopyranoside (2), and 3,4,5-trihydroxy-allylbenzene 3-O-β-d-glucopyranosyl-4-O-β-d-glucopyranoside (3), along with four known phenylpropanoid glycosides (4–7), were isolated from the tuber of Ophiopogon japonicus. Compounds 4–7 were obtained from the genus Ophiopogon for the first time. Their structures were elucidated by spectroscopic methods, including 1D and 2D NMR and HR-ESI-MS.     

麦冬中新成分二氢高异黄酮的研究

目的研究麦冬的化学成分。方法运用多种色谱方法进行分离纯化,根据理化性质和波谱数据鉴定化合物的结构。结果从麦冬80%乙醇提取物的乙酸乙酯萃取部分中分得3个化合物,分别鉴定为:5,7-dihydroxy-6,8-dimethyl-3-(3’,4’-dihydroxybenzyl)chroman-4-one(Ⅰ)、5,7-dihydroxy-3-(4’-hydroxybenzyl)chromone(Ⅱ)和5,7-dihydroxy-6,8-dimethyl-3-(4’-dihydroxy-2’,6’-dimethoxybenzyl)chroman-4-one(Ⅲ)。结论化合物I为一个新的高异黄酮类化合物,化合物Ⅱ为首次从麦冬中分离得到。     

HPLC法同时测定麦冬药材中麦冬皂苷Ra和慈溪麦冬皂苷A的含量

目的:建立以高效液相色谱法测定麦冬药材中麦冬皂苷Ra和慈溪麦冬皂苷A含量的方法。方法:色谱柱为Waters C18(150mm×4.6mm,5μm),流动相为乙腈-水(35∶65),流速为1.0mL·min-1,检测波长为208nm,柱温为室温。结果:麦冬皂苷Ra、慈溪麦冬皂苷A的进样量分别在1.88～9.40μg(r=0.9996)、1.96～9.80μg(r=0.9995)范围内与各自峰面积积分值呈良好线性关系;平均回收率分别为100.2%、99.7%,RSD均为1.7%(n=6)。结论:本方法简便、快速、准确,可用于麦冬药材的质量控制。     

蛇足石杉中一个新的Phlegmariurine型生物碱

目的研究石杉科植物蛇足石杉Huperzia serrata (Thunb.) Trev.的生物碱成分。方法应用各种色谱技术进行分离纯化，经衍生物制备和光谱数据分析鉴定其结构。结果从蛇足石杉中分离得到1个新生物碱。结论该化合物为一新的phlegmariurine型生物碱，命名为8β-羟基马尾杉碱乙（8β-hydroxy phlegmariurine B）。     

川麦冬脱病毒和组织培养技术的研究

采用茎尖分生组织培养技术，获得了川麦冬的脱病毒试管苗。通过川麦冬组织培养技术的正交试验及优化筛选，筛选出最佳的培养基组成，用于脱病毒苗的快速繁殖。建立了川麦冬根尖染色体鉴定的最佳条件。结果表明，川麦冬最适繁殖培养基MS＋BA（6-卞基腺嘌呤）2．0mg／L＋NAA（萘乙酸）0．5mg／L；川麦冬最佳诱导愈伤培养基：MS＋BA1．5mg／L＋IAA（吲哚乙酸）0．1mg／L＋2，4-D（2，4-二氯苯氧乙酸）1．0mg／L；通过在培养基中添加不同浓度生长素，得到适合川麦冬的生根培养基：1／2MS＋IAA0．5mg／L＋ABT0．5mg／L。染色体鉴定结果表明：川麦冬的染色体为2n=68。     

A novel sulphur glycoside from the seeds of Descurainia sophia (L.)

A new sulphur glycoside, named descurainoside (1), and the known compound sinapic acid (2) have been isolated from the seeds of Descurainia sophia (L.) Webb ex Prantl. The structure of 1 has been identified as (1R,6S,8R,9S,10S)-9,10-dihydroxy-4-[(4-hydroxy-3,5-dimethoxyphenyl)methylene]-8-(hydroxymethyl)-2,7-dioxa-5-thiabicyclo[4.4.0]decan-3-one by means of physico-chemical properties and spectroscopic methods (1D and 2D NMR, HRMS, ESI-MS).     

A novel sulphur glycoside from the seeds of Descurainia sophia (L.)

A new sulphur glycoside, named descurainoside (1), and the known compound sinapic acid (2) have been isolated from the seeds of Descurainia sophia (L.) Webb ex Prantl. The structure of 1 has been identified as (1R,6S,8R,9S,10S)-9,10-dihydroxy-4-[(4-hydroxy-3,5-dimethoxyphenyl)methylene]-8-(hydroxymethyl)-2,7-dioxa-5-thiabicyclo[4.4.0]decan-3-one by means of physico-chemical properties and spectroscopic methods (1D and 2D NMR, HRMS, ESI-MS).     

绵麦冬的化学成分研究

目的研究绵麦冬Ophiopogon japonicus(Thunb.)Ker.Gaw l.cv.M ianyangensis的化学成分。方法采用硅胶柱层析和制备薄层层析法进行分离,利用波谱数据鉴定结构。结果从绵麦冬乙酸乙酯提取部分分得4种高异黄酮类化合物,鉴定为oph iopogonanone A、2-′hydroxy-m ethyl oph iopogonone A、oph iopogonanone E和oph iopogonanone F。结论4种化合物均为首次从绵麦冬植物中分得。     

麦冬中几种二氢高异黄酮的立体结构

目的研究麦冬中几种二氢高异黄酮的立体结构。方法采用光谱方法,并用圆二色谱确定立体结构。结果确定6个二氢高异黄酮的立体结构,鉴定结果为:5,7-d ihydroxy-6-m ethyl-8-m ethoxy-3(R)-(2-′hydroxy-4′-m ethoxy-benzyl)chroman-4-one(Ⅰa);5,7-d ihydroxy-6-methyl-8-methoxy-3(S)-(2′-hydroxy-4′-methoxybenzyl)chroman-4-one(Ⅰb);5,7-d ihydroxy-6,8-d im ethyl-3(R)-(3-′m ethoxy-4-′hydroxybenzyl)chrom an-4-one(Ⅱa);5,7-d ihydroxy-6,8-d im ethyl-3(S)-(3′-m ethoxy-4′-hydroxybenzyl)chrom an-4-one(Ⅱb);R-m ethyloph iopogonanone B(Ⅲ)和R-m ethyloph iopogonanone A(Ⅳ)。结论首次确定了6个二氢高异黄酮(Ⅰ~Ⅳ)的立体构型。     

Steroidal glycosides from tubers of Ophiopogon japonicus

A new steroidal saponin, named ophiopogonin E (1), has been isolated from the tubers of Ophiopogon japonicus, along with five known steroidal saponins (2-6). The structure of the new steroidal glycoside was characterized by spectroscopic analysis and acid-catalyzed hydrolysis as pennogenin 3-O-beta-D-xylopyranosyl (1 --> 4)-beta-D-glucopyranoside (1).     

Ophiopojaponin D, a new phenylpropanoid glycoside fromOphiopogon Japonicus Ker-Gawl

A new phenolic glycoside, ophiopojaponin D (1), together with two known compounds, was isolated from the tubers of a famous traditional Chinese herb-Ophiopogon japonicus Ker-Gawl. The spectroscopic and chemical data revealed their structures to be 3-tetradecyloxy-4-hydroxy-allylbenzene-4-O-alpha-L-rhamnopyranosyl (1-->6)-beta-D-glucopyranoside (1), 3, 4-dihydroxy-allylbenzene-4-O-alpha-L-rhamnopyranosyl (1-->6)-beta-D-glucopyranoside (2) and L-pyroglutamic acid (3).     

A novel steroidal alkaloid from Fritillaria shuchengensis

A novel steroidal alkaloid, suchengbeisine (1), along with two known steroidal alkaloids, N-oxide of verticinone (2) and zhebeininoside (3), were isolated from the bulbs of Fritillaria shuchengensis S. C. Chen et S. F. Yin. The structures of these compounds were elucidated by intensive spectroscopic methods, including NMR, IR, CD and MS.     

Stemucronatoside K, a novel C(21) steroidal glycoside from Stephanotis mucronata, inhibited the cellular and humoral immune response in mice

Stephanotis mucronata (Blanco) Merr. has been used for rheumatoid arthritis in Chinese folk herb medicine. Guided by bioactive test, a novel potent immunosuppressive C(21) steroidal glycoside stemucronatoside K (SMK) was isolated from this plant. Its structure was elucidated on the basis of the chemical evidence and extensive spectroscopic methods. We investigated the immunosuppressive effects of SMK in vitro and in vivo. SMK significantly suppressed concanavalin A (Con A)- and lipopolysaccharide (LPS)-stimulated splenocyte proliferation in vitro in a concentration-dependent manner. ICR mice were immunized subcutaneously with OVA on the first day and administered intraperitoneally with SMK at the doses of 2.5, 5, and 10 mg/kg once daily for 10 days. At 24 h after the last administration, mitogen- and OVA-stimulated splenocyte proliferation, the levels of cytokines from splenocytes, and specific antibody titers in serum were measured. SMK significantly inhibited Con A-, LPS- and OVA-induced splenocyte proliferation in the immunized mice in a dose-dependent manner. OVA-specific IgG, IgG1, and IgG2b antibody titers were significantly reduced by SMK compared with the control group. SMK also significantly decreased OVA-induced interleukin-2 (IL-2), interferon-gamma (IFN-gamma), and IL-4 production from splenocytes in the OVA-immunized mice. These results demonstrated that SMK could suppress the cellular and humoral immune response in mice. This study provided evidence to understand the therapeutic effects of S. mucronata and an immunosuppressive natural product compound to further researches to be developed as immunosuppressant.     

A novel flavonoid glycoside from Drymaria diandra

A novel flavonoid glycoside, drymariatin A, was isolated from the whole plants of Drymaria diandra (Caryophyllaceae). By spectroscopic analysis, its structure was elucidated as 6-trans-[2'- O-(alpha-rhamnopyranosyl)]-ethenyl-5,7,4'-trihydroxy-flavone.     

Bebrycoside, a new steroidal glycoside from the Chinese gorgonian coral Bebryce indica

A new steroidal glycoside, bebrycoside (1), was isolated from the South China Sea gorgonian coral Bebryce indica. The structure of 1 was established by extensive spectroscopic analysis, including 1D and 2D NMR data. This was the first time that the steroidal glycoside was isolated from the genus of Bebryce.