前列腺液中MIP-1α、MCP-1表达与慢性前列腺炎的关系

目的 分析前列腺液中巨噬细胞炎性蛋白-1α(MIP-1α)、单核细胞趋化蛋白-1(MCP-1)表达与慢性前列腺炎的关系。方法 回顾性分析2019年10月至2021年10月在唐山市开滦总医院进行诊治的150例慢性前列腺炎患者的临床资料,将其作为观察组,根据美国国立卫生研究院制定的病理分型方法将其分为Ⅱ型组(n=50)、ⅢA组(n=54)、ⅢB组(n=46),另根据慢性前列腺炎症状指数将其分为轻度组(n=48)、中度组(n=62)、重度组(n=40),并选取同期50例体检合格的健康男性作为对照组。比较不同病理分型、不同症状严重程度的慢性前列腺炎患者与健康对照组人群前列腺液中MIP-1α、MCP-1水平差异,分析前列腺液中MIP-1α、MCP-1表达与病理分型及病情进展的关系。结果 Ⅱ型、ⅢA型、ⅢB型组前列腺液中MIP-1α、MCP-1水平均高于对照组,且Ⅱ型、ⅢA型组高于ⅢB型组,其中Ⅱ型组又高于ⅢA型组(P<0.05)。轻、中、重度组前列腺液中MIP-1α、MCP-1水平均高于对照组,且中、重度组均高于轻度组,其中重度组又高于中度组(P<0.05)。经ROC曲线分析,MIP...     

MCP-1的表达与动脉粥样硬化

单核细胞趋化蛋白１（ＭＣＰ－１）能趋化单核细胞在内皮下间隙聚集，这是动脉粥样硬化最早期的病变之一，近年研究表明，内皮细胞、平滑肌细胞、单核细胞和巨噬细胞均能表达ＭＣＰ－１。并认为动脉粥样硬化病变早期的内皮细胞和内皮下巨噬细胞是ＭＣＰ－１的主要来源。     

臭氧髓核消融术应用于腰椎间盘突出症的临床实验研究

目的：探讨臭氧用于腰椎间盘突出症的临床效果及其安全性。方法510例腰椎间盘突出症患者随机分为臭氧组和手法治疗组,对比分析两组疗效及不良反应的发生情况。结果经过1年随访,臭氧组院优250例,良45例,好转46例,无效19例,无并发症出现。手法组院优21例,良28例,好转50例,无效51例,无并发症出现。两组总有效率比较,差异有统计学意义(P<0.05)。结论臭氧髓核消融术治疗腰椎间盘突出症,疗效肯定,并发症少。     

血清MCP-1蛋白水平对肺癌及肺结核患者诊断的临床意义

目的 探讨MCP-1蛋白表达在肺癌及肺结核患者中的临床意义。方法 选取2017年1月~2018年12月我院呼吸科收治的40例肺癌及40例肺结核患者为研究对象,同时选取同期在我院体检健康人群40例为对照组,对比三组血清及PBMC上清液中MCP-1浓度及不同期、不同病理类型肺癌患者血清及PBMC上清液中MCP-1浓度和初治和复治肺结核血清及PBMC上清液中MCP-1浓度。结果 肺结核组及肺癌组血清及PBMC上清液中MCP-1浓度高于对照组,且肺结核组高于肺癌组,差异有统计学意义（P＜0.05）;晚期肺癌患者血清及PBMC上清液中MCP-1浓度分别为（152.71±12.56）pg/ml、（419.52±33.93）pg/ml,均高于早期肺癌患者的（73.21±7.90）pg/ml、（312.60±28.62）pg/ml,差异均有统计学意义（P＜0.05）;三种不同病理类型肺癌患者血清及PBMC上清液中MCP-1浓度比较,差异无统计学意义（P＞0.05）;肺结核复治患者血清及PBMC上清液中MCP-1浓度分别为（173.65±13.22）pg/ml、（520.11±67.28）pg/ml,高于肺结核初治患者的（156.21±9.13）pg/ml、（498.34±50.03）pg/ml,差异有统计学意义（P＜0.05）。结论 血清MCP-1蛋白水平于肺癌及肺结核的发生、病情发展有密切的关系,且随病情的进展会升高,临床可将其作为诊治的重要指标。     

MCP-1在胚胎种植中的作用

趋化因子是一类对中性粒细胞、单核细胞、嗜碱性粒细胞等起趋化和激活作用的多功能糖蛋白,介导炎症和免疫反应,单核细胞趋化蛋白-1（MCP-1）属于CC类趋化因子家族的一员。近年来研究表明,在激素的调节作用下,MCP-1在胚胎种植中发挥重要作用,其机制主要为趋化并激活单核巨噬细胞,参与Th2型免疫偏移及促进血管生成。     

髓核摘除术治疗腰椎间盘突出症的临床观察

自1934年Mixter和Bar首次手术证实腰椎间盘髓核突出引起坐骨神经痛以来,髓核摘除术作为腰椎间盘突出症的标准疗法,得到了广泛的应用。九十年代后,经皮切吸、激光、射频、臭氧及内窥镜等“时髦”疗法。相继应用于临床,以其“微创”的特点受到了医生和患者的青睐,尽管报道的临床疗效不一,但对传统髓核摘除术的地位形成了挑战。     

MCP-1及IL-10在狼疮性肾炎及其相关疾病活动度的临床意义

目的:分析活动期和静止期狼疮性肾炎(LN)患者血清和尿液标本中单核细胞趋化蛋白-1(MCP-1)和IL-10水平的变化并探讨其临床意义。方法:应用ELISA技术对活动期和静止期LN患者血清和尿液中MCP-1和IL-10进行检测,分析LN不同时期MCP-1和IL-10与其他相关指标之间的关系。结果:活动期患者尿液MCP-1水平显著地高于静止期患者,而血清MCP-1两者差异无统计学意义;活动期患者血清IL-10水平显著地高于静止期患者,而尿液IL-10两者差异无统计学意义。结论:尿液MCP-1和血清IL-10水平可能与LN活动存在一定的相关性,有望成为SLE肾脏损害及疗效观察的监测指标。     

氯沙坦对动脉粥样硬化兔的MCP-1及基因表达的实验研究

为了探讨血管紧张素Ⅱ的Ⅰ型受体(AT1R)拮抗剂氯沙坦(Losartan)的抗动脉粥样硬化(AS)作用及观察其是否能抑制单核细胞趋化蛋白-1(MCP-1)的蛋白及基因表达,我们通过高脂饮食及内皮损伤术建立兔AS模型,一组用Losartan(25mg/kg/d),另一组不用,喂养4个月,观察血管内膜的变化;同时做免疫组化及原位杂交,分别观察MCP-1蛋白及基因的表达。结果发现Losartan治疗组兔主动脉内膜面积及内、中膜面积之比均小于未治疗组,其MCP-1的蛋白及mRNA的表达亦明显减少,表明Losartan可通过抑制MCP-1的产生而具有抗AS作用。     

胃癌组织中趋化因子MCP-1的表达与TAMs计数及肿瘤血管生成的相关性

目的检测胃癌组织中MCP-1、TAMs及VEGF的相关性,探讨他们在胃癌发生、发展中的作用。方法采用免疫组化SP法检测20例胃炎组织及50例胃癌组织中MCP-1、TAMs及VEGF的表达及微血管密度(MVD)。结果 (1)胃癌组织中MCP-1、VEGF的表达及TAMs计数均高于对照组;(2)MCP-1与TAMs均与胃癌的组织学分型和分化无关,VEGF与胃癌的分型有关,但与分化无关;(3)MCP-1、VEGF及TAMs均与胃癌的淋巴结转移有关;(4)MCP-1、VEGF及TAMs之间存在正相关关系。结论 MCP-1和TAMs与胃癌的发生发展及血管生成密切相关,可作为判断胃癌预后的生物学指标。     

射频消融髓核成形术在腰椎间盘突出症治疗中的应用

目的 探讨射频消融髓核成形术治疗腰椎间盘突出症的手术技巧、安全性和临床疗效.方法 应用射频消融髓核成形术治疗腰椎间盘突出症186例,并对其临床疗效进行观察分析.结果 所有病例的穿刺及消融过程均顺利完成.对152例患者经历3～24个月随访,根据“中华医学会骨科分会脊柱组腰腿痛手术评定标准“,术后疗效优52例,良78例,可16例,差6例,优良率达85.5%,有效率为96%.出现腰椎间隙感染1例,经卧床、抗炎等治疗而愈.结论 射频消融髓核成形术是治疗腰椎盘突出症安全、有效的微创技术.     

Expression and regulation of monocyte chemoattractant protein-1 by human eosinophils

Several recent studies have identified eosinophils as a cellular source of various cytokines, indicating that eosinophils play not only an effector role, but also a regulatory role within the allergic inflammatory cell network. In this study, we demonstrate that eosinophils can generate and secrete monocyte chemoattractant protein-1 (MCP-1), a prototype of C-C chemokines. Eosinophils generated immunoreactive MCP-1 in response to such diverse stimuli as C5a, formylmethionyl-leucyl-phenylalanine (FMLP) and ionomycin, but MCP-1 production was not induced by interleukin (IL)-1 or tumor necrosis factor-α. C5a- and FMLP-induced eosinophil MCP-1 production was absolutely dependent on pretreatment with cytochalasin B. Eosinophils elaborated significantly more MCP-1 than neutrophils. Immunoreactive MCP-1 was detected at 6 h of incubation with C5a or FMLP. Expression of MCP-1 mRNA reached a maximum within the first 3 h after stimulation and then declined rapidly to a very low and stable level by 18 h. Pretreatment with IL-5 markedly amplified C5a-induced MCP-1 production, and the enhancement occurred at the pretranslational level. Eosinophilactive chemokines such as eotaxin failed to induce MCP-1 generation, even when eosinophils were primed by IL-5. Since MCP-1 exerts a potent histamine-releasing effect on human basophils, our results indicate that eosinophils may regulate basophil mediator release with possible consequent contribution to the pathogenesis of allergic inflammation via a paracrine mechanism.     

MCP-1在妊娠期高血压疾病患者血清中的变化及意义

目的探讨妊娠期高血压疾病患者血清单核细胞趋化蛋白-1(MCP-1)变化的意义。方法用ELISA法对21例妊娠期高血压疾病患者(其中轻度子痫前期12例,重度子痫前期9例)血清MCP-1浓度进行测定,并选择同期30例正常孕妇作为对照组。结果妊娠期高血压疾病患者血清中MCP-1含量显著高于对照组孕妇,并随病情加重呈增加趋势。结论 MCP-1所参与的免疫反应可能是妊娠期高血压疾病的发病机制之一。     

Human monocyte chemoattractant protein-1 (MCP-1)

During the past three years great advances have been made in the chemistry and biology of chemoattractants for human leukocytes. Two chemoattractant cytokines have been isolated, sequenced and cloned, each with distinctive leukocyte attractant specificity. Monocyte chemoattractant protein 1 (MCP-1), the subject of this review by Edward Leonard and Teizo Yoshimura, is secreted by PHA-stimulated mononuclear cells and can be identified by northern blotting in response to LPS or PHA. It attracts monocytes but not neutrophils. In contrast, neutrophil attractant/activation protein (NAP-1) (also known as interleukin 8 (IL-8)) attracts and activates human neutrophils but it is not a chemoattractant for human monocytes. Based on amino acid sequence analysis, each of these attractants has been assigned to one of two distinct families of cytokines that are thought to participate in host defense and inflammatory responses.     

肾小球系膜细胞与单核细胞趋化蛋白-1的关系

肾小球系膜细胞是最活跃的细胞固有成分,单核细胞趋化蛋白-1（monocyte chemoattractant protein-1,MCP-1）是一种对单核细胞具有特异趋化功能的细胞因子,系膜细胞可表达MCP-1及其特异性受体,两者相互作用对多种肾小球病理生理过程发挥重要影响。本文简要地叙述了肾小球系膜细胞及MCP-1的生物学作用,重点分析介绍影响系膜细胞MCP-1表达的因素以及干预治疗对MCP-1表达的影响。     

Expression of monocyte chemoattractant protein-1 and macrophage colony-stimulating factor in normal and inflamed rat testis

Macrophages are numerous in the testicular interstitial tissue under normal conditions and increase during inflammation. The mechanisms involved are poorly characterized. Expression of the macrophage-regulating cytokines monocyte chemoattractant protein (MCP)-1 and macrophage colony-stimulating factor (M-CSF) was examined in the adult rat testis before and after an i.p. injection of an inflammatory stimulus, lipopolysaccharide (LPS). In the normal testis, M-CSF was readily observed using Northern blot and Western blot analysis. In contrast, MCP-1 was not detectable by Northern blot in the normal testis, but was detected using RT-PCR amplification and a sensitive ELISA. After LPS treatment, testicular MCP-1 mRNA and protein expression increased dramatically (up to 400-fold). In-situ hybridization for MCP-1 revealed that production was confined to the interstitium of the inflamed testis, in Leydig cells, peritubular cells, perivascular cells and monocyte-like macrophages, but not in tissue-resident macrophages. Unlike MCP-1, M-CSF mRNA and protein expression in the testis increased only marginally, if at all, after LPS treatment. These results suggest that MCP-1 stimulates the increase in intratesticular macrophages that accompanies LPS-induced inflammation in vivo. Together with M-CSF, MCP-1 may also play a role in maintaining the resident macrophage population of the normal testis.     

Deficiency in monocyte chemoattractant protein-1 modifies lipid and glucose metabolism

We describe the effect of MCP-1 deficiency in mice rendered hyperlipemic by the concomitant ablation of the LDL receptor. The MCP-1(-/-)LDLr(-/-) mice in comparison with LDLr(-/-) mice showed a decreased lipoprotein clearance, derangements in free fatty acids delivery and less glucose tolerance when fed a regular chow, and they showed a partial resistance to alterations in glucose and lipid metabolism induced by dietary fat and cholesterol. They also were less prone to the development of diet-induced obesity. Our results suggest that the role of MCP-1 in metabolism is relevant and that, although new hidden complexities are evident, the function of MCP-1/CCL2 extends far beyond the monocyte chemoattractant effect. Therefore, the regulatory mechanisms influenced by MCP-1 should be fully ascertained to understand the metabolic consequences of inflammation and before considering MCP-1 as a therapeutic target.     

High expression and autoinduction of monocyte chemoattractant protein-1 in scleroderma fibroblasts

Systemic sclerosis (SSc) is a connective tissue disease with unknown etiology characterized by excessive deposition of extracellular matrix in the skin as well as various internal organs. In the early stages of SSc, inflammatory infiltrates of mononuclear cells are found in the dermis, which is associated with increased collagen synthesis produced by activated fibroblasts. Monocyte chemoattractant protein-1 (MCP-1) is a predominant monocyte chemoattractant secreted by a variety of cell types, and recent in vivo and in vitro studies suggest the involvement of MCP-1 in tissue fibrosis. Here we demonstrate that cultured scleroderma fibroblasts, compared to fibroblasts from control skin, spontaneously express significantly elevated MCP-1 levels. Interestingly, exogenously administered MCP-1 stimulated autoinduction of MCP-1 mRNA. This effect was specific to scleroderma fibroblasts and abrogated by actinomycin D. These findings suggest that MCP-1 plays an important role in the induction of scleroderma by MCP-1 release from fibroblasts, which results in recruitment of monocytes to the skin. Moreover, increased responsiveness of scleroderma fibroblasts to MCP-1 could result in a continuation of the fibrotic response.