Emergence of gyrovirus 3 in commercial broiler chickens with transmissible viral proventriculitis

Gyrovirus 3 (GyV3) has been identified in faeces from children with acute gastroenteritis. However, whether GyV3 is prevalent in poultry has not been determined to date. To the best of our knowledge, this study is the first to isolate GyV3 from commercial broiler chickens with transmissible viral proventriculitis (TVP ) in China. The complete genome of the virus shares 98.4% sequence identity with the FecGy strain that causes acute gastroenteritis in children. Epidemiological investigation from 2013 to 2017 revealed that the infection rate of GyV3 reached 12.5% (42/336) in commercial broiler chickens with TVP , indicating that the infection of GyV3 was ubiquitous in chickens. The emergence of GyV3 in commercial broiler chickens should be highly concerning for public health.     

Cerebral salt wasting syndrome in a patient with viral meningoencephalitis

A 53-year-old male was admitted to our hospital for a high fever. He suffered a change in personality, memory loss and disorientation as well. The findings of cerebrospinal fluid showed monocytosis, but the titers of glucose, C1 and ADA were all normal. Although there was no bacterium in the CSF, the patient's electroencephalography finding was abnormal. We diagnosed his condition as viral meningoencephalitis and started treatment with antiviral agents. Blood chemistry showed serum sodium of 130 mEq/l and plasma osmolarity was reduced to 272 mOsm/kg, while urine osmolarity was high at 353 mOsm/kg. Two potential causes of hyponatremia in this patient were the syndrome of inappropriate secretion of antidiuretic hormone (SIADH) or cerebral salt wasting syndrome (CSWS). Physical findings revealed a contracted extracellular fluid volume, strongly suggesting the presence of CSWS. The massive urine sodium loss overcoming sodium intake supported this diagnosis. After treatment with vigorous sodium and volume replacement for over 4 weeks, hyponatremia as well as meningoencephalitis were improved without any complication. To the best of our knowledge, this is the first report on CSWS in a patient with viral meningoencephalitis.     

介绍一种连续皮内缝合新方法

传统的皮内缝合方法是从切口一端经皮内缝至切口另一端，伤口愈合后再从一端将缝线抽出。近年来，我们在临床实践中摸索出新的连续皮内缝合方法，取得满意效果，介绍如下。     

Percutaneous lithotripsy with a new continuous flow pyeloscope

A new percutaneous pyeloscope for the destruction of kidney stones is presented which is based on the continuous flow principle through a double sheath similar to the resectoscopes. The visibility is always good and complications due to high pressure are prevented.     

Intraductal tubular adenoma, pyloric type, of the pancreas: additional observations on a new type of pancreatic neoplasm

Three cases of a distinctive intraductal tubular adenoma, pyloric type, of the main pancreatic duct are reported. The patients, two women and a man, whose ages ranged from 63 to 70 years, complained of abdominal pain attributed to chronic pancreatitis in two patients. The patient with the largest tumor also had symptoms of gastric outlet obstruction. The tumors, two of which arose in the head and one in the tail of the pancreas, led to occlusion and cystic dilatation of the main pancreatic duct. Two adenomas were sessile and one was attached to the wall of the pancreatic duct by a thin fibrous stalk. Microscopically, they were composed of lobules of closely packed tubular glands similar to pyloric glands. In one tumor, nearly all glands were lined by columnar mucin-secreting cells with abundant clear cytoplasm and basally oriented nuclei. In addition to pyloric glands, two adenomas contained glands lined by cells with little or no mucin as well as by pink oncocytic cells. Focal intestinal differentiation was identified in one tumor. Both intracellular and extracellular mucin was detected with the mucicarmine, periodic acid-Schiff, and alcian blue stains. All three adenomas were CK7 positive and CK20 negative. Focal carcinoembryonic antigen linear reactivity along the apical cytoplasm was seen in many cells, but few cells expressed cytoplasmic carcinoembryonic antigen. All three adenomas showed low proliferative activity as measured by the MIB-1 labeling index. The three adenomas were p53 negative and showed loss of DPC4 expression. No endocrine cells were identified in any of the tumors. All patients are alive and symptom free from 4 months to 5 years following surgical treatment.     

Emergence of a Novel Bluetongue Virus Serotype,China 2014

One hundred and twenty‐six blood samples were collected from healthy sheep and goats in Xinjiang, China, during July 2014. Seventy‐three samples (57.93%) were bluetongue virus (BTV) serology‐positive, and 39 samples (30.95%) were BTV NS1 gene‐positive. BTV strain XJ1407 was isolated from the blood of BTV NS1 gene‐positive animals and sequenced. Analysis of its genome sequence suggests that XJ1407 is a novel BTV serotype.     

Collagen type III glomerulopathy: a new type of hereditary nephropathy

A new type of hereditary glomerulopathy was observed in ten children presenting with early and progressive glomerular symptoms, often associated with hypertension. Light microscopy showed a diffuse increase in the mesangial matrix and generalized widening of the capillary walls. Electron-microscopic examination of renal tissue, after phosphotungstic acid treatment, revealed the presence of fibrillar collagen within the mesangial matrix and the subendothelial aspect of the glomerular basement membrane, adjacent to normal lamina densa. Immunohistochemical studies identified the fibrillar collagen not usually present within the glomerular extracellular matrix as type III collagen. Clinical and family studies ruled out the diagnosis of nail-patella syndrome, an autosomal dominant disorder with typical extrarenal symptoms, which is also characterized by the presence of fibrillar collagen within the glomerular basement membranes. The poor renal outcome, the possible extrarenal haematological and pulmonary involvement and the transmission as an autosomal recessive trait strongly suggest that collagen type III glomerulopathy is a new type of hereditary disease. From the high incidence of superimposed haemolytic uraemic syndrome in patients or their siblings, it may be hypothetized that collagen type III glomerulopathy is the underlying defect in some of the familial cases of haemolytic uraemic syndromes.     

一种新发现半月板损伤类型的命名、定义及临床研究

目的发现一种新的半月板损伤类型,命名为纤维松散型半月板撕裂,提出定义并进行临床研究。方法纳入自2012-01—2014-06符合标准的1 008例(1 016膝)因各种原因接受膝关节镜手术的患者,共发现纤维松散型半月板撕裂23例,全部位于外侧半月板前角,其中7例合并半月板囊肿。16例未合并半月板囊肿的患者予以半月板边缘部分修整后(盘状半月板予以成形)由外向内用爱惜邦2号线捆扎缝合。7例合并半月板囊肿患者行外侧半月板前角大部切除术,但尽量保存体部及后角的完整性。结果 1 008例中发现纤维松散型半月板撕裂23例,发生率为2.3%。纤维松散型撕裂是外侧半月板前角特有的损伤类型,而且是常见的损伤类型(35.9%)。22例获得随访13~38个月,平均18.8个月。末次随访时膝关节功能Lysholm评分、IKDC评分较术前明显增加,差异有统计学意义(P0.05)。7例并发半月板囊肿者末次随访时均未复发。结论外侧半月板前角的环状纤维与体部和后角的均匀分布不同,聚集成束,长期的超载负荷使环状纤维束之间松散分离,形成独特的纤维松散型损伤。纤维松散型撕裂是外侧半月板前角常见的损伤类型,也与半月板囊肿多发于外侧半月板前角密切相关。     

腰椎新型钛合金动态内固定系统的稳定性研究

目的 检测由新型钛合金制成的非融合腰椎动态内固定系统的稳定性.方法 收集6具平均6月龄的新鲜小牛腰椎标本,在连续的5种状态下,即完整状态、失稳状态、使用DIFS Ⅰ系统(椎弓根螺钉的弹性模量为75GPa,由新型钛合金制成,其连接棒弹性模量为43GPa,直径为3.5mm)动态固定状态、使用DIFS Ⅱ系统(椎弓根螺钉的弹性模量为75GPa,由新型钛合金制成,其连接棒弹性模量为72GPa,直径为3.5mm)动态固定状态、使用对照组SINO系统(短尾"U"形椎弓根系统,连接棒直径为5.5mm)坚固固定状态,对小牛L2-6施加10N·m的纯力矩,测量标本在三维6个方向前屈、后伸、左右侧屈和左右旋转的活动范围(ROM)和中性区(NZ).结果 在失稳状态下L3,46个方向的ROM和NZ与其他各状态比较,差异均有统计学意义(P<0.05).DIFS Ⅰ系统动态同定与完整状态和SINO系统固定比较差异均无统计学意义.DIFS Ⅱ系统动态固定与完整状态各方向的ROM和NZ比较差异有统计学意义(P<0.05),DIFS Ⅱ系统动态固定与SINO系统固定的各方向ROM和NZ比较差异无统计学意义.L2,3和L4,5的各种状态下6个方向的ROM和NZ比较差异无统计学意义.结论 DIFS Ⅰ系统动态固定既可以维持腰椎的活动性,又可以获得坚强固定的稳定性;DIFS Ⅱ系统动态固定性质更接近坚强固定.