不同剂量乌司他丁对百草枯急性肺损伤大鼠肝肾功能的影响

目的:观察不同剂量乌司他丁(UTI)对百草枯(PQ)急性肺损伤大鼠肝肾功能的影响。方法:将50只SD大鼠随机分为正常对照组(A)、PQ中毒组(B)、小剂量UTI治疗组(C)、中剂量UTI治疗组(D)、大剂量UTI治疗组(E)五组,每组10只。B、C、D、E组大鼠采用PQ一次性灌胃染毒法(80 mg/kg)复制PQ中毒模型,A组采用等量生理盐水灌胃。灌胃30 min后,C组腹腔注射UTI 100 k U/kg,D组腹腔注射UTI 300 k U/kg,E组腹腔注射UTI 600 k U/kg,A、B组注射等量生理盐水。24 h后检测血清ALT、AST、BUN、Cr浓度,光镜下观察肺组织病理学变化,进行肺损伤评分。结果 :C、D、E组和B组比较,大鼠血清ALT、AST、BUN、Cr浓度及肺组织损伤病理评分存在显著差异(P<0.05);C、D、E组之间比较,血清ALT、AST、BUN、Cr浓度无显著差异(P>0.05),肺组织损伤病理评分存在显著差异(P<0.05)。结论:较大剂量UTI对急性PQ中毒大鼠急性肺损伤具有较强保护作用,对肝肾功能无明显影响。     

坎地沙坦干预海仁藻酸致痫大鼠肾脏细胞外信号调节激酶的表达及其机制

目的 探讨坎地沙坦干预后海仁藻酸(kainic acid,KA)致痫大鼠肾脏细胞外信号调节激酶(ERK1/2)的表达及其变化的机制.方法 105只雄性Wistar大鼠随机分为3组:A1-5对照组、B1-5致痫组、C1-5坎地沙坦组,每组各35只,1-5分别表示癫痫后0、2、6、12及24 h.采用立体定位仪下杏仁核内注...     

血清总唾液酸的负标志意义用于肝硬化及肝炎的实验室评价

目的　探讨血清总唾液酸 ( TSA)在肝硬化 ( HC)及肝炎 ( VH)疾病中呈低水平的实验现象 ,并对其临床意义进行实验评价。方法　定量测定 5 98例各类肝脏疾病的血清 TSA和甲胎蛋白 ( AFP) ,并对AFP≥ 2 5μg/L者进行相关统计分析。结果　当 TSA≤ 60 2μg/L和 2 5μg/L 相似文献   

Laparotomic Approach for Collecting Serial Hepatic Biopsies in Rats (Rattus norvegicus) and Mice (Mus musculus)

Researchers often consult with laboratory animal veterinarians for suggestions on how to improve their protocols. We assisted a researcher in performing serial liver biopsies in rats (Rattus norvegicus) to assess the transport of iron on a cellular level. We developed a novel collection approach that used laparotomy through a midline abdominal incision and disposable biopsy punches to obtain liver samples at 3 different times at various intervals. We hypothesized the survival of the subjects undergoing the multiple survival procedures would be independent of the weight loss or gain sustained throughout the study. Although 2 rats died during the study, the results were statistically significant with regard to survival when comparing the Belgrade rats to the Sprague Dawley rats and Swiss Webster mice and were independent of the weight loss or gain incurred during the study. We also performed a pilot study in mice (Mus musculus), using the same method as in the rats, with equivalent results. Our study showed the survival of rodents that underwent multiple laparotomies and liver biopsies was independent of the weight gain or loss throughout the study.Laboratory animal veterinarians often are called on to assist principal investigators with their research projects and procedures. Because of our experiences and backgrounds, we can refine procedures to make them more efficient, more productive, and less intrusive. On one such occasion, a researcher asked our department to assist with the collection of liver biopsies from Belgrade rats (Rattus norvegicus) after they had undergone oral iron loading and treatment with a binding agent. Our review of the available literature revealed a lack of data that supported a single, standard method for collecting multiple liver biopsies in rodents, regardless of whether the technique was invasive or noninvasive. The current pilot study presents a method for collecting a series of 3 liver biopsies from rats and mice that results in minimal hemorrhage, adhesion formation, and scarring. The method uses an open laparotomy technique and a disposable biopsy punch.Our major concerns when we considered performing multiple major surgeries in a single animal were that we adhere to all regulatory documents, maintain the wellbeing of the animal subjects, and provide an adequate scientific justification for the procedures. According to the Guide for the Care and Use of Laboratory Animals,⁵ multiple major surgeries can be performed on a single animal only when they are an essential component of a research protocol, scientifically justified by the investigator, or necessary for clinical reasons.⁵ We ensured that this need was an integral part of the researcher''s protocol and that there was scientific justification for performing multiple procedures on the same subject. Our researchers had a limited number of subjects in their colonies that could be included in their studies, and the biopsies were used to show the effects of diet over time on the liver of each subject. When performing multiple, major surgeries on a single animal, appropriate measures to support the animal''s wellbeing must be implemented throughout the study.Standard technique for collecting liver samples from rodents requires either laparotomy or euthanasia.^4,10,12 Although considered invasive due to the need to incise the skin and musculature of the abdominal body wall to access the abdominal cavity, laparotomies offer better visualization of the tissue and permit the acquisition of larger and more diagnostically useful samples than those obtained by percutaneous needle aspirates.^1,2 In recent years, the use of percutaneous needle biopsies to obtain samples from the liver has increased in favor, but samples collected in this manner may be too small for diagnostic purposes, can be damaged during removal from the needle, and may originate from an inappropriate site.^1,2,6,8,13 Percutaneous needle biopsies can be obtained by using a blind procedure or with the aid of specialized techniques, such as ultrasonography. With a blind technique, the tissue is not visualized, and samples might be acquired from the sites of previous biopsies or from organs other than those intended to be sampled. Ultrasonic assistance is desirable but may not be available at many institutions. Even though laparotomy is invasive, it provides sufficient visualization of the liver so that tissue can be harvested from the most appropriate site, and the use of a biopsy punch to collect hepatic tissue samples provides adequate sample with minimal damage to the remaining liver lobes. Previously described techniques often encouraged the removal of an entire liver lobe or the use of cautery to achieve hemostasis.^1,10,14 However both partial hepatectomy and electrocautery decrease the quantity and quality of liver tissue available for subsequent biopsy procedures.The purpose of the current study was to establish a laparotomy-based approach for collecting liver samples of sufficient size and quality that could be performed multiple times on the same animal and that did not negatively affect the health of the rodents or the histologic quality of the collected tissue. Our results demonstrate that the use of a disposable biopsy punch to obtain liver biopsies in rats (Rattus norvegicus) and mice (Mus musculus) causes minimal damage to the liver, provides adequate tissue specimens to perform diagnostic testing, and does not negatively affect the health of the animal when performed multiple times, thereby enabling researchers to reduce the number of subjects required in their studies.     

油酸致大鼠急性呼吸窘迫综合征氧化应激反应和纳洛酮的拮抗作用

目的探讨纳洛酮对油酸所致大鼠急性呼吸窘迫综合征治疗作用的可能机制。方法将30只健康的Wist—ar大鼠随机分为对照组、油酸组、纳洛酮组。由股静脉注入油酸0．1ml／kg建立ARDS模型,测定肺组织MDA、SOD含量,并计算左肺湿／干重比值．行肺组织病理学及超微结构观察。结果油酸组与对照组比较,左肺湿／干重比值、肺组织中MDA含量明显升高,SOD活性明显降低,肺组织出现明显病理损害,纳洛酮组动物肺损伤轻于油酸组。结论纳洛酮可抑制MDA升高．提高SOD活性．对实验性大鼠油酸型急性呼吸窘迫综合征有一定的治疗作用。     

造影剂致原有肾损害大白鼠急性肾衰的病理生理及病理学研究

研究了造影剂对甘油致肾损害大白鼠的肾毒性。结果示：注射造影剂后２４ｈ，尿γ谷氨酰转肽酶（γ－ＧＴ）、尿蛋白显著增高（Ｐ＜０．０５）；７２ｈ，尿γ－ＧＴ开始恢复，血清尿素氮和血清肌酐显著增高（Ｐ＜０．０１），肾组织肿瘤坏死因子无明显改变，组织学可见肾小管上皮细胞和肾小球损害，肾小管腔阻塞。提示造影剂肾毒性的发病机理可能与直接肾毒性及肾小管阻塞有关。并对尿γ－ＧＴ影响因素进行探讨。     

肾移植术后患者血液他克莫司代谢率(C0/D 值) 对肝肾功能的影响研究

目的　探讨肾移植患者体内他克莫司(Tac) 代谢率(C0/D 值) 和肝肾功能、血浆蛋白的关系,以期对临床的合 理用药提供依据。方法　收集211 例肾移植术后12 个月以上并服用他克莫司的血液标本,化学发光免疫法检测血Tac 浓度;酶法测定TBIL,DBIL,ALT,AST,Urea,Cr,UA,CO2,TP 和ALB;全自动血液分析仪检测HGB。根据 C0/D 值从小到大排列并平均分组,将肾移植受者分为快代谢、中间代谢和慢代谢三组。通过比较三个代谢组的肝、肾 功能以及血浆蛋白水平,探讨Tac 的代谢率对肾移植受者的肝、肾功能的影响。结果　肾移植患者ALT,Urea,Cr, eGFR,CO2,ALB 和HGB 水平在不同Tac 代谢组之间,差异均有统计学意义(F=3.311~6.382, 均P<0.05),而TBIL, DBIL,AST,UA 和TP 水平在不同代谢组之间差异无统计学意义(F=0.9~2.379, 均P>0.05)。结论　在血药浓度控制 相对稳定的情况下,肾移植术后患者血液他克莫司代谢率(C0/D 值) 影响体内ALT,Urea,Cr,eGFR,CO2,ALB 和 HGB 的水平,而对TBIL,DBIL,IBIL,UA 和TP 的影响不大。     

Multiplex size marker (YEAST PLEX) for rapid and accurate identification of pathogenic yeasts

BackgroundMultiple yeast species can cause human disease, involving superficial to deep‐seated infections. Treatment of these infections depends on the accurate identification of causative agents; however, reliable methods are not available in many laboratories, especially not in resource‐limited settings. Here, a new multiplex assay for rapid and low‐cost identification of pathogenic yeasts is described.MethodsA two‐step multiplex assay named YEAST PLEX that comprises of four tubes and identifies 17 clinically important common to rare yeasts was designed and evaluated. The set also provides PCR amplicon of unidentified species for direct sequencing. The specificity of YEAST PLEX was tested using 28 reference strains belonging to 17 species and 101 DNA samples of clinically important non‐target bacteria, parasites, and fungi as well as human genomic DNA. The method was further analyzed using 203 previously identified and 89 unknown clinical yeast isolates. Moreover, the method was tested for its ability to identify mixed yeast colonies by using 18 mixed suspensions of two or three species.ResultsYEAST PLEX was able to identify all the target species without any non‐specific PCR products. When compared to PCR‐sequencing/MALDI‐TOF, the results of YEAST PLEX were in 100% agreement. Regarding the 89 unknown clinical isolates, random isolates were selected and subjected to PCR‐sequencing. The results of sequencing were in agreement with those of YEAST PLEX. Furthermore, this method was able to correctly identify all yeasts in mixed suspensions.ConclusionYEAST PLEX is an accurate, low‐cost, and rapid method for identification of yeasts, with applicability, especially in developing countries.     

HMGB1/RAGE在肝缺血再灌注大鼠的表达及奥曲肽的预处理作用

[目的]探讨HMGB1/RAGE在肝缺血再灌注损伤(HIRI)大鼠中的表达及奥曲肽(Oct)的预处理作用.[方法]健康雄性SD大鼠72只,随机分为假手术(S)组、缺血再灌注(I/R)组、Oct组三组,每组24只.术前30min,Oct组给予含Oct 0.25mg/kg的生理盐水腹腔注射,S组、I/R组同样途径给予相同容积的水,采用Prin-gle's maneuver法建立HIRI模型,在肝脏缺血30min(T1)、再灌注时间1h(T2)、6h(T3)、12h(T4)分别取血检测谷丙转氨酶(ALT)、免疫组化检测高迁移率族蛋白1(HMGB1)的变化.再灌注12h取肝组织,免疫组化检测HMGB1的表达情况,实时荧光定量PCR、Western blot分别检测hMGB1和糖基化终末产物受体(RAGE)的mRNA和蛋白水平的表达.[结果]I/R组、Oct组从T1至T4血清ALT依次升高,均高于S组,且I/R组明显高于Oct组,其差异均有统计学意义(P<0.05);HMGB1水平T1至T3依次升高,T3达到高峰,T4开始下降,但均高于S组,且I/R组明显高于Oct组,其差异均有统计学意义(P<0.05).免疫组化检测HMGB1显示,I/R组、Oct组细胞质阳性表达分别为(6.8±1.2)分、(4.1±1.5)分,明显高于S组(3.2±1.3)分,且I/R组明显高于Oct组,其差异均有统计学意义(P<0.05).与S组比较,I/R肝组织HMGB1、RAGE mRNA和蛋白表达量显著增高(P<0.05);与I/R组比较,Oct预处理后Oct组hMGB1、RAGE mRNA表达量明显降低(P<0.05).[结论]Oct预处理对大鼠HIRI有明显的保护作用,可能与其抑制hMGB1、RAGE的表达有关.     

松郁安神方对PCPA致失眠大鼠睡眠时相的影响

目的:观察松郁安神方对失眠大鼠睡眠时相的影响.方法:采用腹腔注射对氯苯丙氨酸(Para-chlorophenylalanine,PCPA)建立失眠大鼠模型,用松郁安神方进行干预,通过动物睡眠生物解析系统,记录脑电(Electroencephalogram,EEG)和肌电(Electromyogram,EMG),分析睡眠...     

强啡肽致大鼠脊髓损伤与谷氨酸能神经功能关系的研究

为研究脊髓损伤及继发损伤的机理，本实验建立了蛛网膜下腔注射强啡肽A（DynA）致大鼠脊髓损伤的模型。发现兴奋性氨基酸谷氨酸（Glu）受体的亚型-N-甲基-D-天冬氨酸（NMDA）受体拮抗剂DL－2-氨基-5磷酰戊酸（APV）具有对抗DynA致瘫的显著疗效。又进一步观察了不同浓度DynA（1－17）对离体大鼠脊髓片H－Glu释放的影响。发现高钾去极化引起脊髓片释放3H-Glu是一个Ca2 依赖的过程。低浓度DynA（1－17）10-8mol／L，抑制3H－Glu释放．而10-6mol／L。显著增加释放，无致瘫作用的K阿片受体激动剂U50，488H于上述浓度均明显抑制3H－Glu的释放。结果提示，小剂量DynA（1－17）可能通过降低交触前Ca2 内流抑制Glu释放而镇痛，大剂量DynA（1-17）则通过促进Glu释放并作用于NMDA受体而引起神经损伤。     

瑞芬太尼预处理对鼠肝缺血再灌注损伤时肾细胞凋亡及Bcl-2、caspase-3的影响

[目的]探讨瑞芬太尼预处理对大鼠肝脏缺血再灌注(IR)时远隔脏器肾脏细胞凋亡及 Bcl-2、半胱天冬酶家族3(caspase-3)的影响.[方法]健康雄性 SD 大鼠72只,随机分为假手术组(S组)、缺血再灌注组(IR组)、瑞芬太尼组(RPC 组),每组24只.采用Pringle''s maneuver法建立肝IR模型,检测各组缺血30 min(T1)、再灌注时间1 h(T2)、3 h(T3)、6 h(T4)时血清谷丙转氨酶(ALT)、肌酐(Cr)水平,再灌6 h取肾组织光镜观察肾脏病理损伤,同时TUNEL法检测肾脏细胞的凋亡,Western blot检测各组肾组织中B淋巴细胞瘤-2(Bcl-2)和caspase-3蛋白的表达水平.[结果]IR组、RPC组从缺血30 min开始各时点血清ALT、Cr依次升高,均显著高于S组,但RPC组又明显低于IR组,其差异均有统计学意义(P<0.05).S组大鼠肾组织结构正常;IR组可见肾脏组织不同程度的病理损伤:肾小管上皮细胞肿胀、结构欠清、核浓缩、肾小管内有上皮坏死脱落细胞的形成,间质炎性细胞浸润及充血;RPC组损伤明显改善.S组仅有极少量的凋亡阳性细胞,IR组、RPC组凋亡阳性细胞均显著高于S组(P<0.05),且IR组明显高于RPC组,其差异均有统计学意义(P<0.05).与S组比较,肾组织Bcl-2、caspase-3表达水平显著增高(P<0.05);与IR组比较,RPC组Bcl-2水平增高(P<0.05),caspase-3表达量明显下降(P<0.05).[结论]瑞芬太尼对肝IR时肾脏损伤有保护作用,而其保护机制可能与调控肾细胞凋亡和Bcl-2、Caspase-3蛋白的表达有关.     

Phytic Acid Exposure Alters AflatoxinB1-induced Reproductive and Oxidative Toxicity in Albino Rats (Rattus norvegicus)

The increased use of feed in Egypt's aquaculture and animal industries raises concerns about the possible presence of mycotoxins in feedstuffs. The use of alternative medicine, such as botanicals and nutritional supplements, has become popular with inflammatory cases. The present study aimed to testify the role played by phytic acid (IP6) in enhancing the reproductive and oxidative toxicity induced in aflatoxinB1 (AFB1) treated white male albino rats (Rattus norvegicus) throughout treatment and withdrawal periods. One hundred and twenty white male albino rats were grouped into four groups. Group 1, was injected with 300 μg kg⁻¹ body wt of AFB1 once every 3 days for 15 days and left uninjected for another 15 days to study the withdrawal effect. Group 2, was injected with 300 μg kg⁻¹ body wt of AFB1 once every 3 days for 15 days and treated simultaneously with IP6 daily for another 15 days. Group 3, was treated daily with IP6 (40 mg kg⁻¹ body wt) for 15 days and with no treatment for other 15 days. Group 4, injected with equivalent volume of sterile phosphate buffer saline solution as a control group. Sera were taken at the experimental intervals and assayed for testosterone hormone, follicular-stimulating hormone (FSH) and luteinizing hormone (LH) to determine the toxicological impact of AFB1 and the possibility of amelioration by phytic acid on the reproductive performance of the studied animal. The effects of AFB1 treatment on the absolute and relative weight of testis as well as its histopathologic effect on the testis and the possibility of amelioration by IP6 treatment were evaluated. The activities of enzymatic and non-enzymatic anti-oxidants, in addition to lipid peroxidation were measured in the testis’ homogenate of AFB1-treated rats. A decrease in sex hormone levels, an increase in testicular lipid peroxidation product levels and a significant decrease in testicular glutathione content, catalase and total peroxidase and superoxide dismutase activities were recorded. The histopathologic alterations revealed a degeneration and highly mitotic division within the spermatogenic nuclei, in addition to some karyomegaly and nuclear pyknosis. It is concluded that the reduction in the toxicity of free radicals by phytic acid might be responsible for the protective influence observed.     

Comparison of Acoustic Structure Quantification,Transient Elastography (FibroScan) and Histology in Patients with Chronic Hepatitis B and without Moderate to Severe Hepatic Steatosis

The purpose of this study was to compare acoustic structure quantification (ASQ) with transient elastography for staging liver fibrosis. One hundred eighty-two patients with chronic hepatitis B and without moderate to severe hepatic steatosis scheduled for liver biopsy underwent ASQ and transient elastography examinations. All ASQ parameters, including total mode, total average, red mode, red average, red standard deviation, blue mode, blue average, blue standard deviation and focal disturbance (FD) ratio and liver stiffness obtained via transient elastography were found to correlate with fibrosis stage (Spearman's r?=?0.783, 0.791, 0.750, 0.771, 0.544, 0.718, 0.691, 0.439, 0.815 and 0.814, respectively; all p values < 0.001). Among the ASQ parameters, the FD ratio had the highest correlation with the stage of fibrosis. The areas under the receiver operating characteristic curves (AUCs) of FD ratio and liver stiffness were 0.911 and 0.906 for F ≥ F1, 0.918 and 0.882 for F ≥ F2, 0.911 and 0.914 for F ≥ F3 and 0.926 and 0.978 for F?=?F4, respectively. There was no significant difference in AUCs between FD ratio and liver stiffness in predicting different stages of fibrosis (p?=?0.062–0.912). ASQ is a promising technique for assessing liver fibrosis in the absence of moderate to severe hepatic steatosis.     

失语症患者声调障碍的机制和表现

本文主要对失语症患者发生声调错误时的表现及大脑定位进行综述。在简要介绍声调和失语症定义及特点的同时,重点讨论声调的大脑偏侧化。声调既是一种语言信号,又具有音乐旋律的声学特点,其两重性影响声调语言的认知过程,因此国内外对声调研究后显示的偏侧化优势结论并不一致。本文通过总结不同语言正常人声调听感知的偏侧化优势,归纳出现声调障碍的失语症患者的大脑损伤定位,讨论声调于大脑优势侧的定位。本文还对失语症患者的声调障碍特点进行总结和探讨。     

松郁安神方对对氯苯丙氨酸致失眠大鼠血浆中前列腺素D2的影响

目的:观察松郁安神方对对氯苯丙氨酸(Para-chlorophenylalanine,PCPA)致失眠模型大鼠血浆中内源性睡眠促进物质前列腺素D2(Prostaglandin D2,PGD2)的影响.方法:采用腹腔注射对氯苯丙氨酸(PCPA)建立失眠大鼠模型,用高、中、低剂量松郁安神方进行治疗,通过酶联免疫吸附法(ELISA)测定大鼠血浆中前列腺素D2(PGD2)的含量.结果:中、高剂量松郁安神方可提高PCPA失眠模型大鼠血浆中PGD2含量(P<0.05或P<0.01).结论:松郁安神方改善睡眠的机制之一可能是通过调节内源性睡眠促进物质PGD2发挥作用.     

老年人跌倒与平衡及步态异常

老年人跌倒是一种常见的社会流行病,一般是由自身生理、病理、心理因素与存在危险因素的环境相互作用的后果,其中老年人的腿部肌力减退和平衡及步态异常为最重要原因。预防和治疗应该在全面评估的基础上采取以运动治疗为主要内容的综合性措施,其中太极拳运动从多个方面消除老年人跌倒的危险因素,值得临床借鉴。     

异丙酚对内毒素诱导肺损伤MMP-2和MMP-9的影响

目的:研究异丙酚对急性肺损伤时基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)的影响。方法:健康SD大鼠30只随机分为3组:对照组经股静脉注射生理盐水;内毒素组经股静脉注射内毒素5 mg／kg后泵注生理盐水;异丙酚组经股静脉注射内毒素5 mg／kg后泵注异丙酚10 mg.kg1.h1。采取经股静脉注射内毒素复制肺损伤模型(5 mg/ kg)。4 h后放血处死动物,对气管肺泡灌洗液(BALF)行白细胞计数及分类,ELISA法检测血清和BALF中的MMP-2、MMP-9的活性。结果:内毒素组白细胞总数、巨噬细胞、中性粒细胞和淋巴细胞均明显高于其他两组(P<0．01)。异丙酚组白细胞总数明显高于对照组(P<0．01),巨噬细胞、中性粒细胞和淋巴细胞高于对照组(P<0．05);内毒素组血清和BALF中的MMP-2、MMP-9均明显高于其他两组(P<0．01),异丙酚组血清的MMP-2高于对照组(P<0．05)。结论:异丙酚可产生与抑制MMP-2、MMP-9活性相关的肺保护作用。     

Egb761对大鼠海马神经细胞缺血再灌注损伤保护作用的形态学研究

目的：从形态学探讨Egb761对大鼠海马神经细胞缺血再灌注损伤保护作用。方法：用四血管法建立大鼠全脑缺血再灌注模型。24只大鼠随机分为3组（分别为假手术组,缺血再灌注组和Egb761治疗组）。观察海马神经元缺血再灌注后HE染色、TUNEL染色及透射电子显微镜下改变。结果：海马神经原水肿、凋亡等改变在缺血再灌注组明显高于假手术组,Egb761组介于上述两组之间。结论：缺血再灌注后神经细胞发生水肿、凋亡、坏死等形态学改变,Egb761对大鼠海马神经细胞缺血再灌注损伤具有保护作用。     

Cx43 蛋白在丰富环境改善脑外伤大鼠学习记忆能力中的作用

目的观察Cx43 蛋白在丰富环境改善脑外伤(TBI)大鼠学习记忆能力中的作用。方法选用成年Sprague-Dawley 大鼠,采用液压颅脑损伤仪制作TBI 模型,将模型大鼠分为丰富环境刺激组(A 组)、常规饲养组(B 组)、Cx43 特异性反义寡聚核苷酸(Cx43-ASODN)+丰富环境刺激组(C 组)和乱序ODN(scrambled sequence ODN)+丰富环境刺激组(D组),每组6 只。另设6 只正常大鼠作为对照组。C组和D组分别在海马区注射Cx43-ASODN(2 μl/d/rat, 1.5 mmol/L)和ScrbASODN(2 μl/d/rat, 1.5 mmol/L)。饲养14 d,采用水迷宫测试评估大鼠的学习记忆能力。结果B组大鼠潜伏期大于对照组(P<0.05),穿越次数少于对照组(P<0.05)。A组潜伏期小于B组(P<0.05),且从第9 天起与对照组比较无显著性差异(P>0.05);穿越平台次数多于B组,与对照组比较无显著性差异(P>0.05)。C组大鼠的潜伏期大于A组(P<0.05),穿越平台次数少于A组(P<0.05)。结论Cx43 蛋白参与丰富环境改善TBI 大鼠学习记忆能力的调控。