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降钙素基因相关肽在前列腺素介导豚鼠心脏缺血预适应中的作用(英文) 总被引:2,自引:1,他引:1
目的:研究降钙素基因相关肽与前列腺素在豚鼠心脏缺血预适应中的相互作用。方法:采用Langen-dorff方法灌注豚鼠离体心脏。记录心率、冠脉流量、左室内压以及最大变化速率,并测定冠脉流出液中降钙素基因相关肽(CGRP)与6-酮-PGF_(1α)的释放量。结果:内皮素-1(200 pmoL)引起心功能下降,表现为冠脉流量、心率、左室内压及其最大变化速率降低。缺血预适应可明显减轻内皮素-1引起的心脏损伤,同时预适应期间CGRP与6-酮-PGF_(1α)的释放量明显增加。应用辣椒素耗竭内源性CGRP后,缺血预适应的保护作用被取消。选择性CGRP_1受体拮抗剂CGRP_(8-37)100nmol/L也能取消缺血预适应的保护作用。环氧化酶抑制剂吲哚美辛(10μmol/L)可取消缺血预适应的保护作用,同时缺血预适应促进CGRP与6-酮-PGF_(1α)释放的作用也被取消。结论:前列腺素参与了缺血预适应对豚鼠心脏的保护作用,前列腺素的作用是由CGRP所介导。 相似文献
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降钙素基因相关肽:一种调节预适应的内源性中介物(英文) 总被引:9,自引:0,他引:9
心脏遭受短暂缺血或高温处理后均产生早期和延迟保护效应.缺血预适应的心脏保护作用与内源性活性物质有关.辣椒素敏感的感觉神经的主要递质降钙素基因相关肽(CGRP)介导缺血预适应的早期和延迟保护作用.CGRP介导的预适应能保护内皮细胞.热应激的早期和延迟保护也与内源性CGRP释放有关.某些药物如硝酸甘油诱导的预适应可能与其促CGRP释放有关.这些结果表明CGRP可能是一种内源性心肌保护物质,并在预适应的保护效应中起重要作用. 相似文献
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降钙素基因相关肽介导的离体大鼠心脏缺血预处置1肖洲生,李元建2,邓汉武(湖南医科大学药理教研室,长沙410078,中国)关键词降钙素基因相关肽;心肌再灌注损伤;心功能试验;肌酸激酶目的:研究降钙素基因相关肽(CGRP)在离体大鼠心脏缺血预处置(PC)... 相似文献
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降钙素基因相关肽和BIBN4096BS对麻醉大鼠心肌缺血的作用 总被引:5,自引:1,他引:4
WuDM ZwiePA 《Acta pharmacologica Sinica》2001,22(7):588-594
INTRODUCTION Calcitonin gene-related peptide (CGRP) is a 37amino acid peptide that is predominantly synthesized andstored in sensory neurons. It can be released from boththe central and peripheral axons of these neurons.CGRP-containing nerve fibers have been identifiedthroughout the cardiovascular system, in association withblood vessels, in particular the coronary arteries, andaround the sinoatrial and atrioventricular nodes.CGRP is a potent vasodilator peptide and it exerts positivechronotropic and inotropic effects in rats and hu-mans. It has been shown to exert extremely potent 相似文献
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氧自由基或降钙素基因相关肽预处置减轻氧自由基所致心肌损伤 总被引:4,自引:1,他引:3
目的:研究氧自由基(OFR)及降钙素基因肽(CGRP)预处置对OFR所致离体大鼠心脏损伤的拮抗作用。方法:Langendorff法灌流心脏,电解K-H液产生OFR,结果:CGRP或OFR预处置减轻OFR所致心脏收缩功能下降,冠脉流量减少和肌激酶(CK)释放增加,蛋白激酶C(PKC)抑制剂H-7可取消OFR预处置的心脏保护作用(对照组,OFR损伤组,OFR预处置组,H-7加OFR预处置组及H-7组的 相似文献
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硝酸甘油通过舒张血管 ,降低心脏前后负荷及扩张冠状动脉而成为治疗心绞痛的经典药物 ,其作用机制为释放NO ,激活鸟苷酸环化酶升高环鸟苷酸水平而发挥作用。降钙素基因相关肽 (CGRP)为辣椒素敏感感觉神经的主要递质 ,广泛分布于心血管组织。CGRP具有强效舒张血管、强心、保护血管内皮和心肌细胞等复杂的心血管作用 ,其释放受多种内源性活性物质 (包括NO)所调节。最近研究表明 ,CGRP介导硝酸甘油的心血管作用 相似文献
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降钙素基因相关肽介导大鼠后肢缺血预适应的保护作用 总被引:1,自引:0,他引:1
目的:研究降钙素基因相关肽(CGRP)介导缺血预适应对血管内皮的保护。方法:大鼠后肢缺血2h后,观察乙酰胆碱诱导血管内皮依赖性舒张反应。结果:缺血不影去甲蛹 腺素的缩血管效应,但能显著削弱乙酰胆碱的舒血管效应。缺血预适应能阻止长时间缺血对惭酰胆碱舒血管效应的抑制作用,这种保护作用可被反复应用辣椒素耗竭CGRP所取消。急性应用辣椒素促进CGRP释放或外源性应用CGRP均可产生预适应样的保护作用。结论 相似文献
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研究心脏缺血预适应(PC)对溶血性磷脂酰胆碱(LPC)损伤心肌作用的影响,并探讨降钙素基因相关肽(CGRP)在PC中的作用. 离体大鼠心脏Langendorff法灌流,记录心率,冠脉流量,左室压和左室压最大上升速率(+dp/dtmax),并测定灌流液中肌酸磷酸激酶(CPK)含量. 结果显示,LPC能降低各项心功能指标,并使CPK释放增加;PC(缺血5 min, 再灌5min,重复3次)能减轻LPC的损伤作用;PC的心肌保护作用可被选择性CGRP受体拮抗剂CGRP8-37所取消;预先给予CGRP或辣椒素能产生与PC相同的心肌保护作用. 对照组, LPC, PC+LPC, CGRP8-37, CGRP8-37+PC+LPC, CGRP+LPC, CGRP8-37+CGRP+LPC, 辣椒素+LPC组CPK释放量分别为0.26±0.05, 2.30±0.22, 0.25±0.03, 0.30±0.08, 2.60±0.15, 0.24±0.05, 2.70±0.20和0.25±0.07 μmol·min-1·g-1湿组织. 这些结果提示:1) PC对LPC所致心肌损伤具有保护作用;2) PC的保护作用是由CGRP所介导;3) CGRP或辣椒素可模拟PC的保护作用. 相似文献
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降钙素基因相关肽对大鼠小肠缺血预适应的保护作用 总被引:1,自引:0,他引:1
目的探讨降钙素基因相关肽(CGRP)在大鼠小肠缺血预适应中的作用及意义。方法①健康Wistar雄性大鼠,体质量(280±30)g,分为3组(各8只),对照组(CON):仅分离肠系膜上动脉(SMA),不夹闭,观察90 min;缺血再灌组(I/R):分离SMA,夹闭30 min,再灌注60 min,结束实验;缺血预适应组(IP):分离SMA,夹闭SMA 5 min反复3次,然后再夹闭30 min,再灌注60 min,结束实验。②利用放射免疫法测定CGRP含量,以乳酸脱氢酶(LDH)、丙二醛(MDA)含量变化和形态学变化为指标,评价缺血再灌注损伤。结果缺血预适应可明显抑制大鼠小肠缺血再灌注损伤后LDH的水平增高,降低MDA的含量(P<0.01),保护小肠黏膜不受损伤。结论CGRP为大鼠小肠缺血再灌注损伤中关键性介质之一,缺血预适应可提高大鼠小肠缺血再灌注后CGRP的水平,对抗缺血再灌注损伤。 相似文献
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Involvement of alpha-calcitonin gene-related peptide in heat stress-induced delayed preconditioning in rat hearts 总被引:3,自引:0,他引:3
Peng J Lu R Xiao L Deng HW Li YJ 《Clinical and experimental pharmacology & physiology》2002,29(7):569-574
1. Previous studies have shown that hyperthermia is capable of activating capsaicin-sensitive sensory nerves and stimulating the release of neurotransmitters from their peripheral terminals. Calcitonin gene-related peptide (CGRP) has recently been found to participate in delayed cardioprotection in rat isolated hearts. 2. The purpose of the present study was to explore whether the delayed cardioprotection by heat stress in vivo involves the expression and release of CGRP. 3. Sprague-Dawley rats were pretreated with whole-body hyperthermia (rectal 42 degrees C) for 15 min, 24 h before the experiments and then the left main coronary artery of rat hearts was subjected to a 45 min occlusion followed by 3 h reperfusion. The degree of myocardial injury was evaluated by measurement of infarct size and plasma creatine kinase (CK) activity. The plasma levels of CGRP and expression of CGRP (alpha and beta isoforms) mRNA in lumbar dorsal root ganglia at 4, 8, 16 or 24 h after heat stress treatment were measured. 4. Pretreatment with hyperthermia significantly reduced infarct size and CK release. Heat stress also significantly increased plasma concentrations of CGRP and the expression of alpha-CGRP mRNA, but not beta-CGRP mRNA. The effect of heat stress was completely abolished by pretreatment with capsaicin (50 mg/kg, s.c.), which selectively depletes transmitters in capsaicin-sensitive sensory nerves. 5. In summary, the results suggest that the delayed cardioprotection by heat stress involves the synthesis and release of CGRP and that the protection is mainly mediated by the alpha-CGRP isoform. 相似文献
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目的:研究硝酸甘油增强心停搏液的保护作用与促进降钙素基因相关肽释放的关系。方法:在StThomas Hospital心停搏液条件下,离体心脏低温缺血4h后再灌40min,记录心率、冠脉流量及心功能,并测定灌注液中降钙素基因相关肽(CGRP)的浓度及肌酸激酶(CK)的释放量。结果:硝酸甘油(0.1或1μmol/L)改善心功能,降低CK释放,同时促进CGRP的释放。CGRP(5或10nmol/L)也改善心功能及降低CK释放。预先用辣椒素耗竭感觉神经递质后。硝酸甘油的心肌保护和升高灌注液中CGRP浓度作用消失。选择性CGRP受体拮抗剂CGRP_(8-37)也能取消硝酸甘油的心肌保护作用。格列苯脲对硝酸甘油和CGRP的心保护作用均无影响。结论:硝酸甘油增强心停搏液的保护作用是通过内源性CGRP所介导,其保护作用与ATP敏感的钾通道无关。 相似文献
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Monophosphoryl lipid A-induced delayed preconditioning is mediated by calcitonin gene-related peptide 总被引:5,自引:0,他引:5
The delayed preconditioning of the heart by monophosphoryl lipid A is mediated by endogenous nitric oxide (NO), and the cardioprotection afforded by nitroglycerin is related to stimulation of calcitonin gene-related peptide (CGRP) release. The objective of this study was to explore whether improvement of preservation with cardioplegia by monophosphoryl lipid A is mediated by CGRP. In addition, we examined the effect of monophosphoryl lipid A on the tumor necrosis factor-alpha (TNF-alpha) content of myocardial tissues. The isolated rat heart was perfused in the Langendorff mode. Heart rate, coronary flow, left-ventricular pressure, and its first derivatives (+/-dp/dt(max)) were recorded, and plasma levels of NO and CGRP, the release of creatine kinase in coronary effluent and the content of TNF-alpha in myocardial tissues were measured. Hypothermic ischemia for 4 h caused a decline in cardiac function, and an increase in the release of creatine kinase and in the content of TNF-alpha. Pretreatment with monophosphoryl lipid A (500 microg/kg, i.p.) for 24 h improved the recovery of cardiac function and reduced the release of creatine kinase concomitantly with a decrease in the content of cardiac TNF-alpha. Monophosphoryl lipid A markedly increased plasma concentrations of CGRP and NO. After pretreatment with L-nitroarginine methyl ester (L-NAME), the cardioprotection and the increased release of NO and CGRP induced by monophosphoryl lipid A were abolished. Capsaicin also abolished the cardioprotection and the increased release of CGRP induced by monophosphoryl lipid A, but did not affect the content of NO. The results suggest that monophosphoryl lipid A-induced preconditioning enhances preservation with cardioplegia and that the protective effects of monophosphoryl lipid A are related to stimulation of CGRP release. 相似文献
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Lu R Hu CP Peng J Deng HW Li YJ 《Clinical and experimental pharmacology & physiology》2001,28(5-6):392-396
1. It has been suggested that calcitonin gene-related peptide (CGRP) is involved in the protection provided by ischaemic preconditioning in rat hearts and that ischaemic preconditioning is absent in diabetic rat hearts. 2. In the present study, we tested the relationship between sensory nerve function and ischaemic preconditioning in diabetic rats. 3. In 4- and 8-week diabetic rats and age-matched non- diabetic controls, 30 min global ischaemia and 40 min reperfusion caused a significant decrease in cardiac function and a marked increase in creatine kinase (CK) release. Ischaemic preconditioning, by three cycles of 5 min ischaemia and 5 min reperfusion, improved the recovery of cardiac function and decreased CK release during reperfusion in 4-week diabetic rat hearts. However, the cardioprotection afforded by ischaemic preconditioning was lost in 8-week diabetic rat hearts. Pretreatment with CGRP for 5 min also significantly improved the recovery of cardiac function and decreased CK release in rats subjected to 4 or 8 weeks of diabetes. 4. The content of CGRP in the coronary effluent during ischaemic preconditioning was significantly increased in 4-week diabetic rat hearts (P < 0.05). However, only a slight increase in the release of CGRP was shown in 8-week diabetic rat hearts (P > 0.05). 5. In summary, the present results suggest that the protection afforded by ischaemic preconditioning is attenuated in diabetic rats and that the change may be related to the reduction in CGRP release in diabetic rat hearts. 相似文献
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Early and delayed cardioprotection by heat stress is mediated by calcitonin gene-related peptide 总被引:14,自引:0,他引:14
Qiu-Jing Song Y.-J. Li Han-Wu Deng 《Naunyn-Schmiedeberg's archives of pharmacology》1999,359(6):477-483
Brief ischaemia or heat stress protects the myocardium against ischaemia-reperfusion injury. Heat stimulus evokes release
of sensory nerve transmitters, including calcitonin gene-related peptide (CGRP). Since CGRP has been shown to play an important
role in the mediation of ischaemic preconditioning, the present study examined whether early or delayed preconditioning induced
by retrograde hyperthermic perfusion in vitro or by whole-body hyperthemia in vivo also involves endogenous CGRP. Isolated
rat hearts were perfused in the Langendorff mode and subjected to 30 min global ischaemia and 30 min reperfusion. Heart rate,
coronary flow, left ventricular pressure and its first derivatives (±dp/dt) were recorded and the CGRP-like immunoreactivity (CGRP-LI) content and the release of creatine kinase (CK) during reperfusion
were measured. Retrograde hyperthermic perfusion (42 °C) for 5 min improved the recovery of cardiac function, decreased the
release of CK and elevated the content of CGRP-LI in the coronary effluent. CGRP8–37 (10–7 mol/l), a selective CGRP receptor antagonist, abolished the cardioprotection by heat stress. Pretreatment with capsaicin (50
mg/kg s.c.), which specifically depletes sensory nerve transmitter content, abolished both the cardioprotection and the increased
release of CGRP-LI. Whole-body hyperthermia (42 °C for 15 min) caused an increase in the plasma concentration of CGRP-LI.
Early or delayed protection was shown in the hearts obtained from the animals subjected to whole-body hyperthermia 10 min
or 48 h before the experiments. The early or delayed protection by heat stress was also abolished by pretreatment with capsaicin.
The present study suggests that, in the rat, the early and delayed cardioprotection induced by heat stress involves endogenous
CGRP.
Received: 31 December 1998 / Accepted: 6 April 1999 相似文献